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At least 847 records · Page 47

Nonlinear Supersonic Full Potential Analysis

Supersonic Implicit Marching Program (SIMP) applies numerical method, based on conservative form of full potential equation, to problem of threedimensional supersonic flows with embedded subsonic regions. Conservative formulation of problem provides ability to capture shocks and to assess accurately impact of sweep, thickness, and lift for conditions where linear theory unsatisfactory. Technique uses characteristic signal-propagation theory to control density biasing for treatment of shocks (including embedded shocks) and mixed elliptic/hyperbolic crossflow. SIMP written in FORTRAN 77.

Shankar, V.↗

Integrated CFD modeling of gas turbine combustors

3D, curvilinear, multi-domain CFD analysis is becoming a valuable tool in gas turbine combustor design. Used as a supplement to experimental testing. CFD analysis can provide improved understanding of combustor aerodynamics and used to qualitatively assess new combustor designs. This paper discusses recent advancements in CFD combustor methodology, including the timely integration of the design (i.e. CAD) and analysis (i.e. CFD) processes. Allied Signal's F124 combustor was analyzed at maximum power conditions. The assumption of turbulence levels at the nozzle/swirler inlet was shown to be very important in the prediction of combustor exit temperatures. Predicted exit temperatures were compared to experimental rake data, and good overall agreement was seen. Exit radial temperature profiles were well predicted, while the predicted pattern factor was 25 percent higher than the harmonic-averaged experimental pattern factor.

Fuller, E. J.↗

Evolution of a localized Langmuir packet in the solar wind and on auroral field lines

Langmuir emissions in space are reported to be clumpy and intermittent. The high-frequency wave power appears concentrated in spatial packets, whether amidst the solar wind or on auroral field lines. Due to the plasma motion relative to the spacecraft, determining the source for the wave free energy in the three-dimensional electron distribution function has always been difficult, since the unstable features pass by the detector in presumably too short time to be measured. The range of unstable phase velocities and growth rates have generally been estimated rather than determined by unequivocal measurements. The analysis of wave-particle interactions in a space environment has taken recently a new turn with the development of wave correlators on board rockets and satellites. Such instruments seek to identify correlations between the phase of the wave-field and the fluxes of energetic particles. The data interpretation is complex, however, it must be backed by a detailed theoretical understanding of the wave-particle interaction, including the phase relation for inhomogeneous packets. To this end Langmuir packets interacting with fast electrons can be studied in the appropriate regime by means of particle-in-cell simulations, provided that one succeeds in reducing the level of the fluctuations, enhancing the signal-to-noise ratio, and incorporating the appropriate boundary conditions. The first results of such simulations are presented here as a test and expansion of previous analysis.

Roth, I.↗

Membrane-Based Functions in the Origin of Cellular Life

If peptides consist of nonpolar residues only, they become inserted into the nonpolar phase. As demonstrated by the example of the leucine undecamer, such peptides fold into an a-helix as they partition into the nonpolar medium. The folding proceeds through an intermediate, called the 310-helix, which remains in equilibrium with the a-helix. This process represents a simple, protobiologically relevant example of environmentally-mediated self-organization of biological molecules. Once in the nonpolar environment, the peptides can readily change their orientation with respect to the interface from parallel to perpendicular, especially in response to local electric fields. The ability of nonpolar peptides to modify both the structure and orientation with changing external conditions may have provided a simple mechanism of transmitting signals from the environment to the interior of a protocell.

Wilson, Michael↗

Calculations of multiple scattering-induced errors in the GLAS mission

The Geoscience Lidar Altimeter System (GLAS) is a laser altimeter designed to measure temporal changes in the topography and mass balance of the Earth's ice sheets. GLAS is scheduled to be launched as part of the Earth Observing System (EOS) in 2001. Due to the large pulse width of GLAS and the slope (and roughness) of the ice sheets, the mean elevation of the laser's surface spot will be estimated from the centroid of the return pulse. While the accuracy of individual altimetry measurements is expected to be 15 cm, a cross-over technique that averages the elevation differences at selected points on the ice sheets will allow GLAS to detect mean ice elevation changes as small as 2 cm per year. One factor that may degrade the proposed accuracy of the altimeter measurements is the effect of multiple scattering by thin clouds and aerosols over the ice sheets. In the return pulse, some photons from the lidar are slightly deflected by cloud particles but still return to the instrument detector. Since these photons travel a longer path than photons that pass directly to and from the surface, the mean travel time of the pulse is lengthened and the centroid of the return pulse is shifted toward a later time . This study intends to determine the importance of this source of altimetry error in the GLAS measurements. Two methods will be used to estimate path delays in the GLAS lidar for a range of cloud conditions. An analytic expression of a doubly scattered return signal (Eloranta, 1972) will be compared to Monte Carlo simulations that estimate path delays by cloud, aerosol and molecular scattering. The results from both methods will be discussed in relationship to current knowledge of Arctic cloud climatology, and the impact of multiple scattering on GLAS altimeter measurements will be evaluated.

Duda, David P.↗

Molecular Simulations in Astrobiology

One of the main goals of astrobiology is to understand the origin of cellular life. The most direct approach to this problem is to construct laboratory models of protocells. Such efforts, currently underway in the NASA Astrobiology Program, are accompanied by computational studies aimed at explaining self-organization of simple molecules into ordered structures that are capable of performing protocellular functions. Many of these functions, such as importing nutrients, capturing energy and responding to changes in the environment, are carried out by proteins bound to membranes. We use computer simulations to address the following questions about these proteins: (1) How do small proteins self-organize into ordered structures at water-membrane interfaces and insert into membranes? (2) How do peptides form membrane-spanning structures (e.g. channels)? (3) By what mechanisms do such structures perform their functions? The simulations are performed using the molecular dynamics method. In this method, Newton's equations of motion for each atom in the system are solved iteratively. At each time step, the forces exerted on each atom by the remaining atoms are evaluated by dividing them into two parts. Short-range forces are calculated in real space while long-range forces are evaluated in reciprocal space, using a particle-mesh algorithm which is of order O(NInN). With a time step of 2 femtoseconds, problems occurring on multi-nanosecond time scales (10(exp 6)-10(exp 8) time steps) are accessible. To address a broader range of problems, simulations need to be extended by three orders of magnitude, which requires algorithmic improvements and codes scalable to a large number of processors. Work in this direction is in progress. Two series of simulations are discussed. In one series, it is shown that nonpolar peptides, disordered in water, translocate to the nonpolar interior of the membrane and fold into helical structures (see Figure). Once in the membrane, the peptides exhibit orientational flexibility with changing conditions, which may have provided a mechanism of transmitting signals between the protocell and its environment. In another series of simulations, the mechanism by which a simple protein channel efficiently mediates proton transport across membranes was investigated. This process is a key step in cellular bioenergetics. In the channel under study, proton transport is gated by four histidines that occlude the channel pore. The simulations identify the mechanisms by which protons move through the gate.

Pohorille, Andrew↗

Peptide Folding and Translocation Across the Water-Membrane Interface

The ability of small peptides to organize at aqueous interfaces was examined by performing a series of large-scale, molecular dynamics computer simulations of several peptides composed of two amino acids, nonpolar leucine (L) and polar glutamine (Q). The peptides differed in size and sequence of the amino acids. Studies on dipeptides LL, LQ, QL and QQ were extended to two heptamers, LQQLLQL and LQLQLQL, designed to maximize interfacial stability of an alpha-helix and a beta-strand, respectively, by exposing polar side chains to water and nonpolar side chains to a nonpolar phase. Finally, a transition of an undecamer, composed entirely of leucine residues, from a disordered structure in water to an alpha-helix in a nonpolar phase representing the interior of the membrane was investigated. Complete folding of a peptide in solution was accomplished for the first time in computer simulations. The simulations revealed several basic principles governing the sequence-dependent organization of peptides at interfaces. Short peptides tend to accumulate at interfaces and acquire ordered structures, providing that they have a proper sequence of polar and nonpolar amino acids. The dominant factor determining the interfacial structure of peptides is the hydrophobic effect, which is manifested at aqueous interfaces as a tendency for polar and nonpolar groups of the solute to segregate into the aqueous and nonpolar phases, respectively. If peptides consist of nonpolar residue's only, they become inserted into the nonpolar phase. As demonstrated by the example of the leucine undecamer, such peptides fold into an alpha-helix as they partition into the nonpolar medium. The folding proceeds through an intermediate, called 3-10-helix, which remains in equilibrium with the alpha-helix. Once in the nonpolar environment, the peptides can readily change their orientation with respect to the interface from parallel to perpendicular, especially in response to local electric fields. The ability of nonpolar peptides to modify both the structure and orientation with respect to the interface from parallel to perpendicular, especially in response to local electric fields. The ability of nonpolar peptides to modify both the structure and orientation with changing external conditions may have provided a simple mechanism of transmitting signals from the environment to the interior of a cell.

Pohorille, Andrew↗

Plant responses to environmental stress: regulation and functions of the Arabidopsis TCH genes

Expression of the Arabidopsis TCH genes is markedly upregulated in response to a variety of environmental stimuli including the seemingly innocuous stimulus of touch. Understanding the mechanism(s) and factors that control TCH gene regulation will shed light on the signaling pathways that enable plants to respond to environmental conditions. The TCH proteins include calmodulin, calmodulin-related proteins and a xyloglucan endotransglycosylase. Expression analyses and localization of protein accumulation indicates that the potential sites of TCH protein function include expanding cells and tissues under mechanical strain. We hypothesize that at least a subset of the TCH proteins may collaborate in cell wall biogenesis.

Review, Tutorial↗

Validation of a Remote Sensing Based Index of Forest Disturbance Using Streamwater Nitrogen Data

Vegetation disturbances are known to alter the functioning of forested ecosystems by contributing to export ('leakage') of dissolved nitrogen (N), typically nitrate-N, from watersheds that can contribute to acidification of acid-sensitive streams, leaching of base cations, and eutrophication of downstream receiving waters. Yet, at a landscape scale, direct evaluation of how disturbance is linked to spatial variability in N leakage is complicated by the fact that disturbances operate at different spatial scales, over different timescales, and at different intensities. In this paper we explore whether data from synoptic streamwater surveys conducted in an Appalachian oak-dominated forested river basin in western MD (USA) can be used to test and validate a scalable, synthetic, and integrative forest disturbance index (FDI) derived from Landsat imagery. In particular, we found support for the hypothesis that the interannual variation in spring baseflow total dissolved nitrogen (TDN) and nitrate-N concentrations measured at 35 randomly selected stream stations varied as a linear function of the change in FDI computed for the corresponding set of subwatersheds. Our results demonstrate that the combined effects of forest disturbances can be detected using synoptic water quality data. It appears that careful timing of the synoptic baseflow sampling under comparable phenological and hydrometeorological conditions increased our ability to identify a forest disturbance signal.

Eshleman, Keith N.↗

Hybrid data-driven and model-informed online tool wear detection in milling machines

Precision machining tool wear is responsible for low product throughput and quality. Monitoring the tool wear online is vital to prevent degradation in machining quality. However, direct real-time tool wear measurement is not practical. This paper presents residual-based anomaly detection models, combining a hybrid model comprised of a physics-based model and a data-driven model (a decision tree or a neural network) to predict signals of interest (e.g., power or forces) under nominal conditions, followed by Page’s cumulative sum test for detecting tool wear on-line using the computer numerical control machine measurements. The most informative features are ranked using dynamic programming and its approximation variants from real-time measurements and machine settings, such as the width of cut, depth of cut, feed rate and spindle speed, that serve as inputs to the predictive models. The baseline nominal model is incrementally updated with experimental data via a gradient boosted adaptation model to generate the residuals that account for discrepancies between the actual machine data under normal conditions and the baseline nominal model predictions. The hybrid model is validated against 20 Mazak milling machine experimental tests and one Haas run-to-failure experiment. The proposed anomaly detector is applied to synthetic data from simulations of the physics-based model at different operating conditions, measurement noise levels, and tool wear levels, and the methods were able to achieve an overall 92% accuracy in data with 1% noise. The anomaly detection methods based on hybrid model reduced the false alarms of either the data-driven or physical-based models alone, and are found to be capable of good online detection of tool wear.

Online anomaly detection↗

Design and Characterization of the 162.5 MHz RF Component Layout for Fermilab's PIP-II Reference Line

The Proton Improvement Plan II (PIP-II) Reference Line at Fermi National Accelerator Laboratory distributes phase-stable radio-frequency (RF) signals throughout the accelerator. PIP-II requires stable timing and phase reference signals so its accelerating cavities transfer energy to the particle beam at the correct point in each RF cycle. The full system includes 162.5, 325, and 650 MHz sections corresponding to the frequency sections of the PIP-II Linac, with this project focusing on the 162.5 MHz section. The Reference Line must provide a phase stable source signal while responding to phase changes caused by environmental conditions or system drift. Its RF components will be mounted on aluminum heat plates inside a temperature controlled enclosure to further limit temperature-driven phase changes. To prepare the system for manufacture, the project reviewed component functions and dimensions, developed a computer-aided design (CAD) model, and arranged the hardware to support short cable paths, grounding, fastener access, and maintenance. Several full scale, three dimensional printed prototypes allowed the available components to be mounted and inspected. These fit checks revealed mechanical conflicts and guided revisions to component placement, countersink geometry, labeling, and plate thickness. Electrical characterization was also performed on selected RF hardware to compare its measured behavior with the performance metrics that were set for our design. Overall, the project produced a manufacturable 162.5 MHz layout, physical fit-check prototypes, and documented electrical measurements that support review before metal fabrication. The 325 and 650 MHz layouts remain future work because they require additional minor mechanical changes.

Subedi, Harsheet [Unlisted, US, CA] (ORCID:0009000↗

A multi-Physics Experiment for Low-Yield Nuclear Explosion Monitoring

A series of multi-physics experiments, referred to as Physics Experiment 1 (PE1) is underway at the United States’ Nevada National Security Site (NNSS). The PE1 series includes detonations of three underground chemical explosions in P-tunnel, with fully coupled (PE1 A), partially decoupled (PE1 D L ), and fully decoupled (PE1 B) emplacements. Canisters with gas tracers are imbedded in the explosives, and the tracers are released when the canister is destroyed by the detonation. A dedicated electromagnetic (EM) experiment (EMX) generates well-characterized EM signals at an underground location near the chemical explosive experiments. A series of atmospheric experiments (METEX, REACT, and METREX) release smoke and radioactive tracers around Aqueduct Mesa to test gas transport in complex topography. Each of the chemical explosive experiments includes a network of sensors to record seismic, acoustic, and electromagnetic waves, measurement of atmospheric conditions, and air sample collection for measurement of tracer concentration. EMX records EM signals underground and on the surface of Aqueduct Mesa. METEX, REACT, and METREX include measurement of atmospheric condition, as well as tracking smoke releases. REACT and METREX add low-level radioactive gas tracers to the atmospheric releases.

58 GEOSCIENCES↗

Design study of a feedback control system for the Multicyclic Flap System rotor (MFS)

The feasibility of automatically providing higher harmonic control to a deflectable control flap at the tip of a helicopter rotor blade through feedback of selected independent parameter was investigated. Control parameters were selected for input to the feedback system. A preliminary circuit was designed to condition the selected parameters, weigh limiting factors, and provide a proper output signal to the multi-cyclic control actuators. Results indicate that feedback control for the higher harmonic is feasible; however, design for a flight system requires an extension of the present analysis which was done for one flight condition - 120 kts, 11,500 lbs gross weight and level flight.

Weisbrich, R.↗

Automated System Checkout to Support Predictive Maintenance for the Reusable Launch Vehicle

The Propulsion Checkout and Control System (PCCS) is a predictive maintenance software system. The real-time checkout procedures and diagnostics are designed to detect components that need maintenance based on their condition, rather than using more conventional approaches such as scheduled or reliability centered maintenance. Predictive maintenance can reduce turn-around time and cost and increase safety as compared to conventional maintenance approaches. Real-time sensor validation, limit checking, statistical anomaly detection, and failure prediction based on simulation models are employed. Multi-signal models, useful for testability analysis during system design, are used during the operational phase to detect and isolate degraded or failed components. The TEAMS-RT real-time diagnostic engine was developed to utilize the multi-signal models by Qualtech Systems, Inc. Capability of predicting the maintenance condition was successfully demonstrated with a variety of data, from simulation to actual operation on the Integrated Propulsion Technology Demonstrator (IPTD) at Marshall Space Flight Center (MSFC). Playback of IPTD valve actuations for feature recognition updates identified an otherwise undetectable Main Propulsion System 12 inch prevalve degradation. The algorithms were loaded into the Propulsion Checkout and Control System for further development and are the first known application of predictive Integrated Vehicle Health Management to an operational cryogenic testbed. The software performed successfully in real-time, meeting the required performance goal of 1 second cycle time.

Patterson-Hine, Ann↗

Farm-level Interactions Study of a Novel Tri-port Soft-switching Medium-Voltage String Inverter (MVSI) based Large-scale PV-Plus-Storage Farms

A medium-voltage photovoltaic power conversion unit supported by battery based on a novel topology of soft switching solid state transformer is presented in this paper. It can reduce the Levelized Cost of Energy (LCOE) of PVS farm in an extended degree. However, its capability to provide grid-support services, such as frequency response, reactive power support, etc, have not been demonstrated. A series/parallel combination of these modules form a large 20 MVA solar farm. To validate them with reduced computation burden, a small-signal model of MVSI is firstly derived and validated in this paper. The model has been validated to match the full-switch model in both steady-state and dynamic conditions. Next, a farm-level simulation based on the proved small-signal model was implemented to demonstrate grid-support services. Real irradiation date from NREL is used to show the operational capabilities of the system. As a result, the overall farm is simulated based on MATLAB/Simulink domain to check the performance for various cases namely: power transfer from dc-ac side, reactive power support during grid voltage sag and utilization of battery during grid voltage disturbance or partial shading over PV panels.

14 SOLAR ENERGY↗

Development of a worldwide model for Flayer-produced scintillation

An empirical approach to modeling the electron-density irregularities in the F layer of the earth's ionosphere that are primarily responsible for scintillation of transatmospheric VHF-UHF signals has been devised and tested. The work was directed toward two major goals: first, development of a worldwide model for describing the rms fluctuation in signal strength to be expected on an arbitrary satellite-to-earth communication link under average ionospheric conditions; and, second, investigation of the feasibility of similar modeling for description of the complete first-order distribution of signal strength.

Fremouw, E. J.↗

Transcript profiling of plastid ferrochelatase two mutants reveals that chloroplast singlet oxygen signals lead to global changes in RNA profiles and are mediated by Plant U-Box 4

Abstract Background In response to environmental stresses, chloroplasts generate reactive oxygen species, including singlet oxygen ( 1 O 2 ), an excited state of oxygen that regulates chloroplast-to-nucleus (retrograde) signaling, chloroplast turnover, and programmed cell death (PCD). Yet, the central signaling mechanisms and downstream responses remain poorly understood. TheArabidopsis thaliana plastid ferrochelatase two(fc2) mutant conditionally accumulates 1 O 2 , and Plant U-Box 4 (PUB4), a cytoplasmic E3 ubiquitin ligase, is involved in propagating 1 O 2 signals for chloroplast turnover and cellular degradation. Thus, thefc2andfc2 pub4mutants are useful genetic tools to elucidate these signaling pathways. Previous studies have focused on the role of 1 O 2 in promoting cellular degradation infc2mutants, but its impact on retrograde signaling from mature chloroplasts (the major site of 1 O 2 production) is poorly understood. Results To gain mechanistic insights into 1 O 2 signaling pathways, we compared transcriptomes of adult wt,fc2, andfc2 pub4plants. The accumulation of 1 O 2 infc2plants broadly repressed genes involved in chloroplast function and photosynthesis, while inducing genes and transcription factors involved in abiotic and biotic stress, the biosynthesis of jasmonic acid (JA) and salicylic acid (SA), microautophagy, and senescence. Elevated JA and SA levels were observed in 1 O 2 -stressedfc2plants.pub4reversed most of this 1 O 2 -induced gene expression and reduced the JA content infc2plants. Thepub4mutation also blocked JA-induced senescence pathways in the dark. However, fc2 pub4 plantsmaintained constitutively elevated levels of SA even in the absence of bulk 1 O 2 accumulation. Conclusions Together, this work demonstrates that infc2plants, 1 O 2 leads to a robust retrograde signal that may protect cells by downregulating photosynthesis and ROS production while simultaneously mounting a stress response involving SA and JA. The induction of microautophagy and senescence pathways indicate that 1 O 2 -induced cellular degradation is a genetic response to this stress, and the bulk of this transcriptional response is modulated by the PUB4 protein. However, the effect ofpub4on hormone synthesis and signaling is complex and indicates that an intricate interplay of SA and JA are involved in promoting stress responses and programmed cell death during photo-oxidative damage.

Plant Sciences↗

A microcomputer based frequency-domain processor for laser Doppler anemometry

A prototype multi-channel laser Doppler anemometry (LDA) processor was assembled using a wideband transient recorder and a microcomputer with an array processor for fast Fourier transform (FFT) computations. The prototype instrument was used to acquire, process, and record signals from a three-component wind tunnel LDA system subject to various conditions of noise and flow turbulence. The recorded data was used to evaluate the effectiveness of burst acceptance criteria, processing algorithms, and selection of processing parameters such as record length. The recorded signals were also used to obtain comparative estimates of signal-to-noise ratio between time-domain and frequency-domain signal detection schemes. These comparisons show that the FFT processing scheme allows accurate processing of signals for which the signal-to-noise ratio is 10 to 15 dB less than is practical using counter processors.

Horne, W. Clifton↗