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All sourcesNASA NTRSDOE OSTI
NASA NTRS2016

Icebreaker-3 Drill Integration and Testing at Two Mars-Analog Sites

A decade of evolutionary development of integrated automated drilling and sample handling at analog sites and in test chambers has made it possible to go 1 meter through hard rocks and ice layers on Mars. The latest Icebreaker-3 drill has been field tested in 2014 at the Haughton Crater Marsanalog site in the Arctic and in 2015 with a Mars lander mockup in Rio Tinto, Spain, (with sample transfer arm and with a prototype life-detection instrument). Tests in Rio Tinto in 2015 successfully demonstrated that the drill sample (cuttings) was handed-off from the drill to the sample transfer arm and thence to the on-deck instrument inlet where it was taken in and analyzed ("dirt-to-data").

astrobiology↗
DOE OSTI2024

Summertime Continental Shallow Cumulus Cloud Detection Using GOES‐16 Satellite and Ground‐Based Ceilometer at North Alabama

Abstract Accurate simulations of boundary layer cloud processes remain challenging in Earth system modeling. Observations are essential to evaluate and improve models of such processes. This study introduces a comprehensive validation framework for a satellite‐based detection algorithm of continental shallow cumulus (ShCu) clouds during the daytime, which was initially developed using ground‐based observations of stereo cameras at the Department of Energy Atmospheric Radiation Measurement (ARM) Southern Great Plains site (J. Tian et al., 2021, https://doi.org/10.3390/rs13122309 , 2022, https://doi.org/10.1029/2021gl097070 ). To validate this algorithm, the framework employs ground‐based ceilometer measurements from North Alabama (NA) where ShCu populations are prevalent. This study first generates clear‐sky surface reflectance maps at NA and identifies ShCu pixels with a detection threshold using Geostationary Operational Environmental Satellite (GOES) reflectance data. The obtained cloud fractions (CFs) are then compared against CFs from a ground‐based ceilometer, considering factors such as observed area differences, satellite parallax issue, and systematic biases. We found that with a detection threshold (∆R) of 0.05, the ShCu detection algorithm is effective for NA, enabling the reproduction of hourly ShCu CFs using GOES. Our framework is straightforward and easily repeatable to evaluate the effectiveness of a ∆R threshold for detecting ShCu clouds in various geographic regions where ceilometers are deployed. This satellite detection of ShCu provides a crucial regional context for ground‐based measurements, facilitating the tracking of convection initiation and its coupling with land surface conditions. Integrating localized ground‐based and regional satellite data will enhance our ability to conduct thorough studies of cloud morphology and land‐atmosphere interactions in North Alabama.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Seismoacoustic Signatures Observed During a Long-Term Deployment of Infrasound Sensors at the Nevada National Security Site

Earthquakes have repeatedly been shown to produce inaudible acoustic signals (<20 Hz), otherwise known as infrasound. These signals can propagate hundreds to thousands of kilometers and still be detected by ground-based infrasound arrays depending on the source strength, distance between source and receiver, and atmospheric conditions. Another type of signal arrival at infrasound arrays is the seismic induced motion of the sensor itself, or ground-motion-induced sensor noise. Measured acoustic and seismic waves produced by earthquakes can provide insight into properties of the earthquake such as magnitude, depth, and focal mechanism, as well as information about the local lithology and atmospheric conditions. Large earthquakes that produce strong acoustic signals detected at distances greater than 100 km are the most commonly studied; however, more recent studies have found that smaller magnitude earthquakes ($M$ w <2.0) can be detected at short ranges. In that vein, this study will investigate the ability for a long-term deployment of infrasound sensors (deployed as part of the Source Physics Experiments [SPE] from 2014 to 2020) to detect both seismic and infrasonic signals from earthquakes at local ranges (<50 km). Methods used include a combination of spectral analysis and automated array processing, supported by U.S. Geological Survey earthquake bulletins. Further, this investigation revealed no clear acoustic detections for short range earthquakes. However, secondary infrasound from an $M$ w 7.1 earthquake over 200 km away was detected. Important insights were also made regarding the performance of the SPE networks including detections of other acoustic sources such as bolides and rocket launches. Finally, evaluation of the infrasound arrays is performed to provide insight into optimal deployments for targeting earthquake infrasound.

58 GEOSCIENCES↗
DOE OSTI2025

Barge Site - Avian Radar System / Derived Data

This is a combined data set of 67,410 bird/bat tracks from an avian radar system deployed on a research barge (MERLIN True3D, DeTect, Panama City, Florida, USA) and concurrent wind measurements from two scanning lidars (WindCube v2.1, Vaisala, Vantaa, Finland, and Halo XR+, Halo Photonics, Lannion, France). The research barge (16.5 m x 61 m) was deployed as part of the Wind Forecast Improvement Project (WFIP-3) off the northeast coast of the United States south of Massachusetts (40.9 deg N, 70.79 deg W). This data set comprises 5 weeks of data between August 27th 2024 and September 27th 2024. Radar data were provided by DeTect and Lidar data were accessed through the Wind Data Hub (wfip3/barg.WINDPROF.z01.a0) The data have been filtered and sorted into two size groups ("big" and "small") based on a clustering approach. See Snortland, A., Clerc, J., Hein, C., & Cotter, E. (2025). Wind as Driver of Bird and Bat Abundance, Flight Direction, Altitude, and Speed on the North Atlantic Shelf. arXiv preprint arXiv:2511.14983 for complete details. Data are provided in 2 files: "Birds" and "Birds_hourly" Birds: This file contains information about each of the 67,410 flying animal tracks detected by the radar during the data collection period, including parameters measured by the radar and wind information interpolated from the lidar wind measurements. We note that the raw radar dataset contained 301,618 tracks; tracks in this processed dataset were filtered based on the requirements described in Snortland et al. (2025). Birds_hourly: This file contains timeseries of the number of tracks detected per hour over the course of the data collection period, including wind conditions and sun position for each hour. These data were used for generalized additive modeling in Snortland et al. (2025).

17 WIND ENERGY↗
DOE OSTI2026

High Contrast Pattern Projection To Enable Background Oriented Schlieren Based Air Leak Detection Through Any Interior Or Exterior Building Surface

Air leakage in buildings wastes an estimated 4 quads of energy per year in the United States. Finding and sealing leakage sites is critical in existing buildings. Previous work has shown that background oriented Schlieren (BOS) imaging can be used to visualize air leakage but requires the leak to exit through a high contrast surface like a brick wall. To remedy this, different techniques of projecting various high contrast patterns on low contrast building surfaces like interior gypsum walls or vinyl siding were investigated. For each technique, the background quality was measured and compared to an ideal printed random dot background. The background quality metrics were correlated with the measured visualization metrics to understand which metrics are most important for maximizing air leak visualization performance. In this work, leakage visualization performance is presented for these various projected backgrounds with an aim to expand the building surfaces suitable for the BOS leak detector.

Jatana, Gurneesh [ORNL] (ORCID:0000000288903225)↗
NASA NTRS2006

An Overview of Moessbauer Mineralogy at Gusev Crater, Mars

The Mars Exploration Rover (MER) Spirit landed on the plains of Gusev Crater on 4 January 2004 [1]. The scientific objective of the Moessbauer (MB) spectrometer on Spirit is to provide quantitative information about the distribution of Fe among its oxidation and coordination states, identification of Fe-bearing phases, and relative distribution of Fe among those phases. The speciation and distribution of Fe in Martian rock and soil constrains the primary rock types, redox conditions under which primary minerals crystallized, the extent of alteration and weathering, the type of alteration and weathering products, and the processes and environmental conditions for alteration and weathering.We discuss the Fe-bearing phases detected by Spirit s MB instrument during its first 540 sols of exploration [2,3]. Spirit roved eastward across the plains from its landing site to the Columbia Hills during the first approx.150 sols. Rocks are unweathered to weakly weathered olivine basalt, with olivine, pyroxene (Ol > Px), magnetite (Mt), and minor hematite (Hm) and nanophase ferric oxide (npOx) as their primary Fe-bearing minerals. Soils are generally similar basaltic materials, except that the proportion of npOx is much higher (up to approx.40%). NpOx is an oct-Fe3+ alteration product whose concentration is highest in fine-grained soils and lowest in rock interiors exposed by grinding with the Rock Abrasion Tool (RAT). Spirit explored the lower slopes of the Columbia Hills (West Spur) during sols approx.150-320. West Spur rocks are highly altered, even for interior surfaces exposed by grinding (Fe3+/FeT approx.0.56-0.84). High concentrations of npOx, Hm, and Mt are present. One rock (Clovis) contains significant quantities of goethite (alpha-FeOOH; approx.40% of total Fe). The detection of goethite is very significant because it is a mineralogical marker for aqueous alteration.

Klingelhoefer, G.↗
NASA NTRSDate not supplied

Common Scientific and Technological Interests Between Astrobiology and Space Biology

The disciplines of astrobiology (AB) and space biology (SB) clearly have common interests, however they have not been pursued jointly. SB and AB are inextricably linked, both intellectually and technologically. They can now be effectively linked operationally. Cross-cutting joint collaborations will enhance innovation and increase cost effectiveness. Session topics include joint science questions, technologies, instrumentation, and missions. Examples include life detection, overlapping planetary protection concerns, biofilms, radiation, hyper- and hypogravity, applications of artificial intelligence and machine learning, interoperable databases, facilities (i.e., spacecraft, lunar surface efforts, simulation chambers, analog sites, etc.), training opportunities, and other topics relevant to AB and SB joint ventures. We welcome contributions on this very broad topical area to facilitate cross-fertilization of these disciplines that are of great importance to NASA.

astrobiology↗
DOE OSTI2025

CROCUS Forward Scatter Disdrometer Data at Argonne National Laboratory Prairie Site

The Vaisala FD70 is a multi-parameter present weather and visibility sensor designed to measure precipitation type, intensity, and visibility with high accuracy in diverse environmental conditions. It uses a combination of forward-scatter measurement and optical disdrometer technologies to detect drop size, fall speeds, and optical properties, enabling the classification of various precipitation types such as rain, snow, sleet, and freezing rain along is visibility estimates. The FD70 provides quantitative estimates of liquid-equivalent precipitation rate and meteorological optical range (MOR), supporting applications in meteorological research, aviation, and road weather monitoring. These measurements are collected at the Argonne Testbed for Multiscale Observational Science (ATMOS), a 20 acre prairie site at Argonne National Lab, located in Lemont, IL. Data is available in netcdf format. Each file contains one second interval data, for approximately 24 hrs each day. File naming convention includes the project (CROCUS), location (ATMOS), instrument name, data level (raw, a1), and date (year, month, day).

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS2022

Phase II and III Solid Waste Management Unit Assessment and Confirmatory Sampling Report for Per- and Polyfluorinated Alkyl Substances, Potential Release Location 237, Kennedy Space Center, Florida

Per- and polyfluoroalkyl substances (PFAS) Phase II and III Solid Waste Management Unit (SWMU) Assessment (SA) and Confirmatory Sampling (CS) were conducted at the John F. Kennedy Space Center (KSC), Potential Release Location (PRL) 237, located in Merritt Island, Brevard County, Florida for the National Aeronautics and Space Administration (NASA) under Contract 80KSC019D0010/80KSC019F0289. A Phase I SA and CS Report was previously submitted that identified 33 locations of concern (LOCs) at KSC associated with the storage, use, or release of materials containing PFAS. The objectives of the Phase II and III SA and CS were to: - Complete site and source characterization of identified LOCs; - Identify areas of potential concern (AOPCs) where past or present activities may have resulted in a release of PFAS to the environment; - Qualitatively characterize migration potential of released PFAS to environmental media (primarily soil, groundwater, surface water, and sediment); and - Identify potential receptors for exposure to PFAS-impacted environmental media. Surface water samples were collected from LOCs/AOPCs and locations on the KSC periphery during PFAS CS Phases I, II and III. Including duplicate samples, 143 environmental samples were analyzed for 14 PFAS compounds and 104 environmental samples were analyzed for 18 PFAS compounds. Ten of the analytes on the 14 compound list were detected at least once in surface water at KSC, with a wide range in magnitude of detections. The State of Florida has surface water screening levels (SWSLs) for perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) in surface water, both for human and ecological receptors. The human health screening levels are lower than the ecological ones and are used in this report. PFOA was not detected in surface water at concentrations exceeding the SWSL at any LOC or Area of Potential Concern (AOPC). PFOS was detected in surface water at concentrations exceeding the SWSL at 28 LOCs/AOPCs, plus 42 locations on the KSC periphery. The data suggest a wide distribution of PFAS, particularly PFOS, in surface water but the sources of surface water contamination are not yet adequately understood. Sediment samples were collected during Phases II and III but not Phase I. Including duplicate samples, 104 environmental samples were analyzed for 14 PFAS compounds and 10 environmental samples were analyzed for 18 PFAS compounds. Twelve compounds were detected in sediment at KSC, with a wide range in magnitude of detections. There are currently no screening levels or cleanup target levels (CTLs) for sediment. The need to delineate and further characterize PFAS in sediment will be evaluated for those sites that move forward into Site Assessment. Groundwater samples were collected via DPT from LOCs/AOPCs and locations on the KSC periphery during PFAS CS Phases I, II and III. Including duplicate samples, 812 environmental DPT groundwater samples were analyzed for 14 PFAS compounds and 247 environmental samples were analyzed for 18 PFAS compounds. Twelve of the 14 compounds were detected in groundwater at KSC, with a wide range in magnitude of detections. The State of Florida has provisional groundwater cleanup target levels (GCTLs) for PFOA, PFOS and the additive concentration of PFOA+PFOS. PFOA was detected in groundwater at concentrations exceeding its provisional GCTL at 16 LOCs/AOPCs, with no exceedances on the KSC periphery. PFOS was detected in groundwater at concentrations exceeding its provisional GCTL at 30 LOCs/AOPCs, plus two locations on the KSC periphery. PFOA+PFOS was detected in groundwater at concentrations exceeding the provisional GCTL at 31 LOCs/AOPCs, plus two locations on the KSC periphery. Soil samples were collected during Phases II and III but not Phase I. Including duplicate samples, 292 environmental samples were analyzed for 14 PFAS compounds and 80 environmental samples were analyzed for 18 PFAS compounds. Fourteen compounds were detected in soil at KSC, with a wide range in magnitude of detections. The State of Florida has provisional soil cleanup target levels (SCTLs) for PFOA and PFOS. There were no PFOA results above its provisional SCTL. There was one LOC with a result above the provisional SCTL for residential settings. Recommendations are presented for each LOC and AOPC.

PFAS↗
DOE OSTI2020

Detection of very-high-energy gamma-ray transients with monitoring facilities

ABSTRACT The observation of very-high-energy γ-rays (VHE γ-rays, $E \gt 100\,$ GeV) has ledto the identification of extremely energetic processes and particle-acceleration sites both within our Galaxy and beyond. It is expected that VHE facilities, such as the Cherenkov Telescope Array, will explore these sources with an unprecedented level of detail. However, the transient and unpredictable nature of many important processes means that their observation requires the development of proper monitoring strategies. In this study, we estimate the properties of VHE transients that can be effectively detected by monitoring facilities. We use data collected by the Fermi-LAT instrument during its monitoring campaign to select events that are probably associated with VHE emission. We use this sample to estimate the frequency, the luminosity and the time-scales of various transients, focusing on blazar flares and gamma-ray bursts. We discuss how the balance between the field of view, sensitivity and duty cycle of an observatory affects the likelihood of detecting transients that occur at the inferred rates, and we conclude by describing the contribution that current and near-future monitoring facilities can make to the identification and study of VHE transient emission.

La Mura, G.↗
DOE OSTI2025

Chelation ion chromatography as an automated, and cost-effective analytical technique for REE determination: method development and applications

Rare earth elements (REEs), as critical minerals, have important uses in modern energy and technologies, yet are vulnerable to potential supply chain disruptions. To establish domestic REE supply chain, efficient REE detection methods for resource characterization and mineral processing will be needed to accelerate innovations for domestic REE recovery. This study developed a rapid, novel, and cost-effective for REE detection method using ion chromatography (IC) for aqueous samples. Various REE-targeted eluent gradients and post-column agent compositions were tested on the chelation ion chromatography (CIC) with UV-vis detector for optimal separation and quantification of REEs within approximately 20 min. The single-channel pump to deliver the post-column solution to UV-vis detector was replaced with a 4-channel gradient pump, to increase operation and maintenance efficiencies. After method optimization, resulting calibration curves for more than ten REEs achieved high coefficients of determination (R2>0.999) and low relatively standard deviations (below 3.24%), demonstrating sub-ppm level detection limits (0.0897 to 0.1149 mg/L). The reliability of the CIC method was validated through comparison with inductively coupled plasma mass spectrometry (ICP-MS), showing strong agreement in REE recovery from certified standards. The impact of metal ions and salts on REE recovery using CIC was also systematically investigated. CIC consistently exhibited reliable performance in the presence of salt solutions such as NaCl and Na₂SO₄ (up to 10,000 mg/L). Our study also found the presence of high concentrations of Al ions (at 10,000 mg/L) significantly influenced REE determination, and elevated concentrations of Ca ions affected the recovery of specific REEs, including La, Ce, and Pr. The CIC method was further tested on REE-containing eluents from solvent extraction tests out of fly ash leachates. REE detection from these real processing fluids were reported to achieve 90% to 100% recovery rate from our IC method, compared to ICP-MS results. This study underscores the potential of CIC as a reliable and efficient alternative for REE determination in complex matrices. It also highlights the importance of minimizing select interfering metal ions in solutions to ensure accurate results. The REE CIC method presents a promising, low-maintenance, salt-tolerant, and cost-effective alternative to traditional analytical methods for REE analysis.

detection of rare earth elements (REE)↗
DOE OSTI2023

Combining field and remote sensing data to estimate forest canopy damage and recovery following tropical cyclones across tropical regions

Abstract As tropical forests cycle the most water and carbon, it is crucial to understand the short- and long-term effects of intensifying cyclones on these ecosystems. Soil nutrient status has been shown to moderate forest cyclone responses using field litterfall measurements, but litterfall is one of the multiple cyclone impact metrtics, which may or may not be correlated with one another or with site nutrients. We used remotely sensed vegetation indices to quantify immediate damage and two-year recovery for 42 cases across nine tropical forests in Hawaii, Puerto Rico, Mexico, Australia, and Taiwan affected by 12 cyclones between 2004 and 2017. We tested whether changes in leaf area index (LAI) and enhanced vegetation index (EVI) correlated with changes in litterfall observations and how changes varied with total soil phosphorus (P) concentrations across regions. We compared cyclone-induced changes and recovery of LAI and EVI to litterfall observations compiled in a pantropical meta-analysis. We found large variation in changes in LAI and EVI across forests, with the greatest reductions in LAI (−77%) and EVI (−77%) in Mexico (Jalisco) and Puerto Rico, respectively. LAI ( r = −0.52) and EVI ( r = −0.60) changes correlated with those in litterfall across cases. Post-cyclone data showed recovery of LAI by four months, EVI by two months, and litterfall by ten months. We detected larger changes in LAI and EVI in forests with higher soil P, but these relationships were not significant when accounting for cyclone and site as random effects. Principal component analyses indicated a regional clustering of cases related to their contrasting cyclone regimes, with the frequency and intensity of cyclone events negatively correlated. Overall, remote sensing observations complement but do not substitute for ground observations that reveal cyclone damage and post-cyclone recovery in tropical forests, and soil phosphorus moderates some but not all metrics of stability in response to cyclones.

Bloom, Dellena E. (ORCID:0000000205981747)↗
NASA NTRS2024

Launch Complex 39A, SWMU 008 2023 Long-Term Monitoring Report Kennedy Space Center, Florida

The 2023 Long-Term Monitoring (LTM) Report (LTMR) presents the annual and semi-annual groundwater monitoring results for Launch Complex 39A (LC39A), Solid Waste Management Unit (SWMU) 008, at Kennedy Space Center (KSC), Florida. The reporting period covered under this LTMR is from January to December 2023, and represents the first year of site-wide monitoring following Air Sparge (AS) system shutdown at the end of 2022. AS system operation at LC39A began operation in 2015. The AS system consisted of a total of 173 AS wells ranging in depth from 11 to 37 feet below land surface. The overall remedial objective for the AS Interim Measure was to actively decrease concentrations of COCs to less than their respective Florida Department of Environmental Protection Natural Attenuation Default Concentrations, so that LC39A can transition into an LTM program. This objective was achieved in 2022, with 2023 marking Year 1 of the LTM program to monitor the residual groundwater plume. VOC results indicate that COC concentrations have continued to decrease or stabilize, with no rebound observed since AS system shutdown. Vinyl chloride was the only COC detected above its GCTL, with the highest concentration detected at 8.0 μg/L. PFAS results detected 15 different PFAS compounds (out of 32 analyzed) between the six monitoring wells sampled. Two PFAS compounds, perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), currently have FDEP Provisional GCTLs of 70 nanograms per liter (ng/L). PFOS was detected in one of the six samples above the FDEP Provisional GCTL at a concentration of 200 ng/L. PFOA was not detected above the FDEP Provisional GCTL in any of the six samples. PFOS and PFOA also currently have United States Environmental Protection Agency (USEPA) Maximum Contaminant Levels (MCLs) of 4 ng/L. PFOS was detected in all six samples above the USEPA MCL. PFOA was detected in three of the six samples above the USEPA MCL. Three other PFAS compounds, perfluorohexanesulfonic acid (PFHxS), perfluoro-n-nonanoic acid (PFNA), and hexafluoropropylene oxide dimer acid (GenX), currently have USEPA MCLs of 10 ng/L. PFHxS, PFNA, and GenX were not detected at concentrations greater than their respective USEPA MCLs in any of the six wells. Additional PFAS sampling will occur as part of a PFAS Site Assessment to be conducted in the future. Based on Year 1 LC39A LTM results, recommendations for 2024 are to continue with the second year of LTM in 2024 with the same monitoring well network as Year 1 (18 wells). The three wells that were on a semi-annual schedule will transition to an annual schedule to align with the remaining wells. The next annual event is scheduled for May 2024. The three wells that were found to be inadvertently destroyed will be properly abandoned and a replacement well in the vicinity of former monitoring well 21ST-MW0030I will be installed. This new well will be added to the LTM sampling plan upon installation.

Deborah M. Wilson↗
DOE OSTI2024

Hydrogen–Deuterium Exchange Mass Spectrometry Identifies Local and Long-Distance Interactions within the Multicomponent Radical SAM Enzyme, PqqE

Interactions among proteins and peptides are essential for many biological activities including the tailoring of peptide substrates to produce natural products. The first step in the production of the bacterial redox cofactor pyrroloquinoline quinone (PQQ) from its peptide precursor is catalyzed by a radical SAM (rSAM) enzyme, PqqE. We describe the use of hydrogen–deuterium exchange mass spectrometry (HDX-MS) to characterize the structure and conformational dynamics in the protein–protein and protein–peptide complexes necessary for PqqE function. HDX-MS-identified hotspots can be discerned in binary and ternary complex structures composed of the peptide PqqA, the peptide-binding chaperone PqqD, and PqqE. Structural conclusions are supported by size-exclusion chromatography coupled to small-angle X-ray scattering (SEC-SAXS). HDX-MS further identifies reciprocal changes upon the binding of substrate peptide and S-adenosylmethionine (SAM) to the PqqE/PqqD complex: long-range conformational alterations have been detected upon the formation of a quaternary complex composed of PqqA/PqqD/PqqE and SAM, spanning nearly 40 Å, from the PqqA binding site in PqqD to the PqqE active site Fe 4 S 4 . Interactions among the various regions are concluded to arise from both direct contact and distal communication. The described experimental approach can be readily applied to the investigation of protein conformational communication among a large family of peptide-modifying rSAM enzymes.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2024

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

To address the growing threat of global antimicrobial resistance, a one-health approach is needed to understand the complex socioecological cycling of antibiotic resistance genes. In this study, a metagenomics approach using DNA shotgun sequencing, metagenome assembly, and antibiotic resistance gene (ARG) annotation was used to examine seasonal patterns in the abundance of mobile colistin resistance (mcr) gene variants in bioaerosols and liquid sludge in three wastewater treatment plants (WWTPs). ARGs represented 0.2–0.8 and 0.1–0.2% of the bioaerosol and liquid sludge metagenomes, respectively, while mcr genes represented 0–0.3 and 0–0.5% of the identified ARGs in bioaerosol and liquid sludge metagenomes. Seven of the ten known mcr variants were detected in wastewater bioaerosol and liquid samples, with mcr-5 and mcr-8 being the most prevalent across all seasons and sites. Furthermore, additional functional and taxonomic annotation of mcr-containing metagenomic contigs showed that mcr genes were often located on contigs with other co-occurring ARGs and mobile genetic elements and may be harbored by opportunistic human pathogens and other bacterial taxa not previously associated with mcr genes. Atmospheric dispersion modeling showed that mcr-containing bioaerosols can be transported kilometers away from the WWTPs, resulting in the possible dissemination of these ARGs into surrounding environments and communities.

Antimicrobial agents↗
DOE OSTI2021

Climatology of Melting Layer Heights Estimated From Cloud Radar Observations at Various Locations

A melting layer (ML) detection algorithm for cloud radar with polarimetric capability was developed and applied to the cloud radar data collected from five different sites around the world for several years. The retrieved melting layer top height (MLH) showed a very good correspondence with the ECMWF Reanalysis 5 zero degree level data for all five sites. The ML characteristics were distinctively different for different sites, revealing climatological characteristics of ML forming clouds in different regions. Generally, ML tended to occur more frequently in summer than in winter except for a maritime site, where low stratiform clouds formed frequently in summer, the top of which might be lower than the freezing level. In contrast, at two Arctic sites, ML occurred almost exclusively in summer because it was too cold to have an ML in the other seasons. The MLH also varied significantly from site to site but generally was higher during warmer seasons. Based on MLH, two new indices, bulk temperature lapse rate (BLR) and relative depth (RD) of liquid cloud below MLH (RD) were developed, which were useful to explain the environmental characteristics of the five sites. BLR generally increased with the surface temperature at all sites except at the marine site that showed an opposite trend, where a unique synoptic pattern in winter generated high BLR in this cold season. Finally, these findings confirm that studies on thermodynamic structures using cloud radars can be broadened, taking advantage of BLR and RD information, as these indices can represent environmental thermodynamic characteristics of the clouds that have ML.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Tuning of charge density wave transitions in LaAu x Sb 2 by pressure and Au stoichiometry

Two charge density wave transition can be detected in La Au Sb 2 at ~ 110 and ~ 90 K by careful electrical transport measurements. Whereas control of the Au site occupancy in La Au x Sb 2 (for 0.9 ≲ x ≲ 1.0 ) can suppress each of these transitions by ~ 80 K , the application of hydrostatic pressure can completely suppress the lower transition by ~ 7.5 kbar and the upper transition by ~ 17 kbar . Here, clear anomalies in the resistance as well as the magnetoresistance are observed to coincide with the pressures at which the charge density wave transitions are driven to zero.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗