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At least 829 records · Page 46

A Genome-Scale Metabolic Model of Anabaena 33047 to Guide Genetic Modifications to Overproduce Nylon Monomers

Nitrogen fixing-cyanobacteria can significantly improve the economic feasibility of cyanobacterial production processes by eliminating the requirement for reduced nitrogen. Anabaena sp. ATCC 33047 is a marine, heterocyst forming, nitrogen fixing cyanobacteria with a very short doubling time of 3.8 h. We developed a comprehensive genome-scale metabolic (GSM) model, iAnC892, for this organism using annotations and content obtained from multiple databases. iAnC892 describes both the vegetative and heterocyst cell types found in the filaments of Anabaena sp. ATCC 33047. iAnC892 includes 953 unique reactions and accounts for the annotation of 892 genes. Comparison of iAnC892 reaction content with the GSM of Anabaena sp. PCC 7120 revealed that there are 109 reactions including uptake hydrogenase, pyruvate decarboxylase, and pyruvate-formate lyase unique to iAnC892. iAnC892 enabled the analysis of energy production pathways in the heterocyst by allowing the cell specific deactivation of light dependent electron transport chain and glucose-6-phosphate metabolizing pathways. The analysis revealed the importance of light dependent electron transport in generating ATP and NADPH at the required ratio for optimal N2 fixation. When used alongside the strain design algorithm, OptForce, iAnC892 recapitulated several of the experimentally successful genetic intervention strategies that over produced valerolactam and caprolactam precursors.

59 BASIC BIOLOGICAL SCIENCES↗

Processes controlling helium bubble dynamics at varying temperatures in simulated radioactive materials

Alpha-radiation damage is known to degrade materials exposed to harsh radiation environments via swelling and embrittlement by nano-scale helium bubbles. How these bubbles grow under varying temperature is essential to understanding and predicting material failure and optimization. Using in situ X-ray scattering, we probed ≈1012 bubbles before, during and after annealing at different temperatures in He-implanted aluminum to reveal a critical growth activation temperature that depends strongly on the helium implantation conditions. An analysis of the data aided by our molecular dynamics simulations identifies multiple temperature-dependent bubble growth mechanisms above and below the critical growth activation temperature. Moreover, the irreversible bubble growth that occurs after annealing for ≈4 h was evaluated using dislocation dynamics and found to negligibly affect the yield strength but may affect subsequent bubble growth at temperature. These in situ results provide valuable insight into bubble growth never observed before and reveal, for the first time, bubble shrinkage after cooling.

36 MATERIALS SCIENCE↗

Rapid Process to Generate Beam Envelopes for Optical System Analysis

The task of evaluating obstructions in the optical throughput of an optical system requires the use of two disciplines, and hence, two models: optical models for the details of optical propagation, and mechanical models for determining the actual structure that exists in the optical system. Previous analysis methods for creating beam envelopes (or cones of light) for use in this obstruction analysis were found to be cumbersome to calculate and take significant time and resources to complete. A new process was developed that takes less time to complete beam envelope analysis, is more accurate and less dependent upon manual node tracking to create the beam envelopes, and eases the burden on the mechanical CAD (computer-aided design) designers to form the beam solids. This algorithm allows rapid generation of beam envelopes for optical system obstruction analysis. Ray trace information is taken from optical design software and used to generate CAD objects that represent the boundary of the beam envelopes for detailed analysis in mechanical CAD software. Matlab is used to call ray trace data from the optical model for all fields and entrance pupil points of interest. These are chosen to be the edge of each space, so that these rays produce the bounding volume for the beam. The x and y global coordinate data is collected on the surface planes of interest, typically an image of the field and entrance pupil internal of the optical system. This x and y coordinate data is then evaluated using a convex hull algorithm, which removes any internal points, which are unnecessary to produce the bounding volume of interest. At this point, tolerances can be applied to expand the size of either the field or aperture, depending on the allocations. Once this minimum set of coordinates on the pupil and field is obtained, a new set of rays is generated between the field plane and aperture plane (or vice-versa). These rays are then evaluated at planes between the aperture and field, at a desired number of steps perceived necessary to build up the bounding volume or cone shape. At each plane, the ray coordinates are again evaluated using the convex hull algorithm to reduce the data to a minimal set. When all of the coordinates of interest are obtained for every plane of the propagation, the data is formatted into an xyz file suitable for FRED optical analysis software to import and create a STEP file of the data. This results in a spiral-like structure that is easily imported by mechanical CAD users who can then use an automated algorithm to wrap a skin around it and create a solid that represents the beam.

Howard, Joseph↗

Delving into the depths of NGC 3783 with XRISM II. Cross-calibration of X-ray instruments used in the large, multi-mission observational campaign

Context. Accurate X-ray spectroscopic measurements are fundamental for deriving basic physical parameters of the most abundant baryon components in the Universe. The plethora of X-ray observatories currently operational enables a panchromatic view of the high-energy emission of celestial sources. However, uncertainties in the energy-dependent calibration of the instrument transfer functions (e.g. the effective area, energy redistribution, or gain) can limit - and historically, did limit - the accuracy of X-ray spectroscopic measurements. Aims. We revised the status of the cross-calibration among the scientific payload on board four operation missions: Chandra, NuSTAR, XMM-Newton, and the recently launched XRISM. XRISM carries the micro-calorimeter Resolve, which yields the best energy resolution at energies ≥2 keV. For this purpose, we used the data from a 10-day-long observational campaign targeting the nearby active galactic nucleus NGC 3783, carried out in July 2024. Methods. We present a novel model-independent method for assessing the cross-calibration status that is based on a multi-node spline of the spectra with the highest-resolving power (XRISM/Resolve in our campaign). We also estimated the impact of the intrinsic variability of NGC 3783 on the cross-calibration status due to the different time coverages of participating observatories and performed an empirical reassessment of the Resolve throughput at low energies. Results. Based on this analysis, we derived a set of energy-dependent correction factors of the observed responses, enabling a statistically robust analysis of the whole spectral dataset. They will be employed in subsequent papers describing the astrophysical results of the campaign. Aims. We revised the status of the cross-calibration among the scientific payload on board four operation missions: Chandra, NuSTAR, XMM-Newton, and the recently launched XRISM. XRISM carries the micro-calorimeter Resolve, which yields the best energy resolution at energies ≥2 keV. For this purpose, we used the data from a 10-day-long observational campaign targeting the nearby active galactic nucleus NGC 3783, carried out in July 2024. Methods. We present a novel model-independent method for assessing the cross-calibration status that is based on a multi-node spline of the spectra with the highest-resolving power (XRISM/Resolve in our campaign). We also estimated the impact of the intrinsic variability of NGC 3783 on the cross-calibration status due to the different time coverages of participating observatories and performed an empirical reassessment of the Resolve throughput at low energies. Results. Based on this analysis, we derived a set of energy-dependent correction factors of the observed responses, enabling a statistically robust analysis of the whole spectral dataset. They will be employed in subsequent papers describing the astrophysical results of the campaign.

Active Galactic Nuclei, individual: NGC 3783↗

Wind stress over the Arabian Sea from ship reports and Seasat scatterometer data

Seasat scatterometer data over the Arabian Sea are used to build wind-stress fields during July and August 1978. They are first compared with 3-day wind analyses from ship data along the Somali coast. Seasat scatterometer specifications of 2-m/s and 20-deg accuracy are fulfilled in almost all cases. The exceptions are for winds stronger than 14 m/s, which are underestimated by the scatterometer by 15 percent. Wind stress is derived from these wind data using a bulk formula with a drag coefficient depending on the wind intensity. A successive-correction objective analysis is used to build the wind-stress field over the Arabian Sea with 2 x 2-deg and 6-day resolution. The final wind-stress fields are not significantly dependent on the objective analysis because of the dense coverage of the scatterometer. The combination of scatterometer and coastal ship data gives the best coverage to resolve monsoon wind structures even close to the coast. The final wind stress fields show wind features consistent with other monthly mean wind stress field. However, a high variability is observed on the 6-day time scale.

Perigaud, C.↗

Refraction of Sound Emitted Near Solid Boundaries from a Sheared Jet

A mathematical model is developed to describe the sound emitted from an arbitrary point within a turbulent flow near solid boundaries. A unidirectional, transversely sheared mean flow is assumed, and the cross-section of the cold jet is of arbitrary shape. The analysis begins with Lilley's formulation of aerodynamic noise and, depending upon the specific model of turbulence used, leads via Fourier analysis to an expression for the spectral density of the intensity of the far-field sound emitted from a unit volume of turbulence. The expressions require solution of a reduced Green's function of Lilley's equation as well as certain moving axis velocity correlations of the turbulence. Integration over the entire flow field is required in order to predict the sound emitted by the complete flow. Calculations are presented for sound emitted from a plugflow jet exiting a semi-infinite flat duct. Polar plots of the far-field directivity show the dependence upon frequency and source position within the duct. Certain model problems are suggested to investigate the effect of duct termination, duct geometry, and mean flow shear upon the far-field sound.

Dill, Loren H.↗

Application of machine learning approaches in the analysis of mass absorption cross-section of black carbon aerosols: Aerosol composition dependencies and sensitivity analyses

Physics-based models typically require an in-depth understanding of a phenomenon and assumptions of the underlying process(es), which are often hard to obtain in practice, whereas data-driven machine learning models learn the structure and patterns in the training data without any prior theoretical assumptions and then use inference to develop useful predictions. A novel machine learning-based algorithm has been previously developed for the prediction of black carbon mass absorption cross-section (MAC BC ) and applied to a variety of different atmospheric environments. In contrast to light scattering theories which require assumptions about the underlying physics, this algorithm uses time-series data of aerosol properties to estimate the temporally-varying MAC BC at 870 nm. Here, we analyze our algorithm and discuss the influence of aerosol optical properties (such as Ångström exponents and single scattering albedo) and chemical composition on the model outputs and the associated accuracy. Additionally, we conduct sensitivity analyses on our models to understand how the predictions change in response to different sets of input variables. Our support vector machine (SVM) for regression model is the least sensitive to variations in the input variables, although all models tend to exhibit a degradation to their accuracy when scattering Ångström exponents are less than one.

54 ENVIRONMENTAL SCIENCES↗

Using Digital Radiography To Image Liquid Nitrogen in Voids

Digital radiography by use of (1) a field-portable x-ray tube that emits low-energy x rays and (2) an electronic imaging x-ray detector has been found to be an effective technique for detecting liquid nitrogen inside voids in thermal-insulation panels. The technique was conceived as a means of investigating cryopumping (including cryoingestion) as a potential cause of loss of thermal insulation foam from space-shuttle external fuel tanks. The technique could just as well be used to investigate cryopumping and cryoingestion in other settings. In images formed by use of low-energy x-rays, one can clearly distinguish between voids filled with liquid nitrogen and those filled with gaseous nitrogen or other gases. Conventional film radiography is of some value, but yields only non-real-time still images that do not show time dependences of levels of liquids in voids. In contrast, the present digital radiographic technique yields a succession of images in real time at a rate of about 10 frames per second. The digitized images can be saved for subsequent analysis to extract data on time dependencies of levels of liquids and, hence, of flow paths and rates of filling and draining. The succession of images also amounts to a real-time motion picture that can be used as a guide to adjustment of test conditions.

Cox, Dwight↗

Optimized Profile Retrievals of Aerosol Microphysical Properties from Simulated Spaceborne Multiwavelength Lidar

This work is an expanded study of one previously published onretrievals of aerosol microphysical properties from space-borne multiwavelengthlidarmeasurements. The earlier studiesand this one weredone in the framework of the NASA Aerosol-Clouds-Ecosystems (now the Aerosol Clouds Convection and Precipitation) NASA mission. The focus here is on the capabilities of a simulated spacebornemultiwavelengthlidar system for retrieving aerosol complex refractive index (m = mr+ imi) and spectral single scattering albedo (SSA(λ)), although other bulk parameters such as effective (reff) radius and particle volume (V) and surface (S) concentrations are also studied. The novelty presented here is the use of recently published, case dependent optimized-constraints on the microphysical retrievals using three backscattering coefficients (β) at 355, 532 and 1064 nm and two extinction coefficients (α) at 355 and 532 nm, typically known as the stand-alone 3β+2α lidar inversion. Case-dependent optimized-constraints (CDOC) limit the ranges of refractive index, both real (mr) and imaginary (mi) parts, and of radii that are permitted in the retrievals. Such constraints are selected directly from the 3β+2α41measurements through an analysis of the relationship between spectral dependence of aerosol extinction-to-backscatter ratios (LR) and the Ångström exponent of extinction. The analyses presented here for different sets ofsize distributions and refractive indices reveal that the direct determination of CDOCareonly feasible for cases where the uncertaintiesin the input optical data areless than 15 %.Forthe same simulated spacebornesystem and yield than in Whiteman et al., (2018), we demonstrated that the use of CDOC as essential for the retrievals of refractive index and also largely improved retrieval of bulk parameters. A discussion of the global representativeness of CDOC is presented using simulated lidar data from a 24-hour satellite track using GEOS model output to initialize the lidar simulator.We found that CDOCare representative of many aerosol mixtures in spite of some outliers (e.g. highly hydrated particles) associatedwith the assumptions of bimodal size distributions and of the same refractive index for fine and coarse modes. Moreover, sensitivity tests performed using synthetic data reveal that retrievals of imaginary refractive index (mi) and SSA are extremely sensitive to β(355).

NASA Aerosol-Clouds-Ecosystems↗

Gallium oxide (Ga 2 O 3 ) energy dependent scintillation response to fast neutrons and flash gamma-rays

Gallium oxide is a newly emerged ultrawide bandgap (4.9 eV) semiconductor that is suitable as a combined electronics and radiation detection platform. We have experimentally demonstrated fast neutron and gamma-ray scintillation from Czochralski-grown β-Ga 2 O 3 in a recent series (October 2023) of experiments at the unmoderated pulsed neutron spallation source located at the Los Alamos Neutron Science Center. Using the neutron time-of-flight (TOF) technique and a fast-gated intensified CCD camera, we observed energy-dependent neutron scintillation for neutron energies ranging from 1 to 400 MeV, including the 14.1 MeV neutron energy relevant to D–T fusion. Neutron flux is quantified and calibrated by cascading the scintillator after the fission chamber, enabling a detailed analysis of temporal and energy-dependent characteristics of the scintillation events. Further, a pronounced scintillation signal from the spallation gamma flash with a temporal full width of half maximum of ~4 ns is indicative of the material’s rapid response. Neutron energy dependent scintillation is observed using the TOF method at a 22.6-m distance from the neutron source. These results highlight the possibility of developing a Ga 2 O 3 based fusion neutron diagnostic platform integrated with both scintillation and electronics functions on the integrated chip scale.

47 OTHER INSTRUMENTATION↗

A meta-evaluation of the quality of reporting and execution in ecological meta-analyses

Quantitatively summarizing results from a collection of primary studies with meta-analysis can help answer ecological questions and identify knowledge gaps. The accuracy of the answers depends on the quality of the meta-analysis. We reviewed the literature assessing the quality of ecological meta-analyses to evaluate current practices and highlight areas that need improvement. From each of the 18 review papers that evaluated the quality of meta-analyses, we calculated the percentage of meta-analyses that met criteria related to specific steps taken in the meta-analysis process (i.e., execution) and the clarity with which those steps were articulated (i.e., reporting). We also re-evaluated all the meta-analyses available from Pappalardo et al. to extract new information on ten additional criteria and to assess how the meta-analyses recognized and addressed non-independence. In general, we observed better performance for criteria related to reporting than for criteria related to execution; however, there was a wide variation among criteria and meta-analyses. Meta-analyses had low compliance with regard to correcting for phylogenetic non-independence, exploring temporal trends in effect sizes, and conducting a multifactorial analysis of moderators (i.e., explanatory variables). In addition, although most meta-analyses included multiple effect sizes per study, only 66% acknowledged some type of non-independence. The types of non-independence reported were most often related to the design of the original experiment (e.g., the use of a shared control) than to other sources (e.g., phylogeny). We suggest that providing specific training and encouraging authors to follow the PRISMA EcoEvo checklist recently developed by O’Dea et al. can improve the quality of ecological meta-analyses.

59 BASIC BIOLOGICAL SCIENCES↗

Secondary Ion Mass Spectroscopy

Secondary Ion mass Spectroscopy (SIMS), as the name suggests, involves characterizing metallic and other materials trough the spectroscopic analysis of secondary ions emanating from the surface of the material to be characterized by the impact of the high energy primary ions. The primary ion source including the choice of its gun, voltage and current can be selected and used depending on the purpose of the analysis. In most instruments more than one primary ion gun is lined up to the sample stage and can be activated with selected accelerating parameters (voltage and beam intensity). The impingement of primary ions on to the sample surface generates positive, negative or neutral ions, electrons, atoms and atomic clusters. Majority of these sample fragments being neutral, could not be utilized as such fragments cannot be manipulated through the use of electromagnetic or electrostatic lenses. Secondary ions that are positively or negatively charged possess large variation in velocity, charge, and mass. These ionic fragments eventually travel through a system of several lenses in very high vacuum to reach detector/counter. Relative amounts of alloying elements or impurities in an alloy can be calculated from the counts of related ions accumulated in the detector/counter.

Panda, Binayak↗

Analytical simulation of weld effects in creep range

The inelastic analysis procedure used to investigate the effect of welding on the creep rupture strength of a typical Liquid Metal Fast Breeder Reactor (LMFBR) nozzle is discussed. The current study is part of an overall experimental and analytical investigation to verify the inelastic analysis procedure now being used to design LMFBR structural components operating at elevated temperatures. Two important weld effects included in the numerical analysis are: (1) the residual stress introduced in the fabrication process; and (2) the time-independent and the time-dependent material property variations. Finite element inelastic analysis was performed on a CRAY-1S computer using the ABAQUS program with the constitutive equations developed for the design of LMFBR structural components. The predicted peak weld residual stresses relax by as much as 40% during elevated temperature operation, and their effect on creep-rupture cracking of the nozzle is considered of secondary importance.

Dhalla, A. K.↗

Radar Analysis and Visualization Environment (RAVEN): Software for polarimetric radar analysis

Imaging radar data provides information about the geometric and dielectric properties of the Earth's surface. The Jet Propulsion Laboratory (JPL) polarimetric Airborne Synthetic Aperture Radar (AIRSAR) is currently obtaining imaging radar data for use in geologic, vegetation, snow and ice, and ocean studies. In the near future, the Shuttle Imaging Radar C (SIR-C/X-SAR) and the Earth Observing System Synthetic Aperture Radar (EOS SAR) will also collect polarimetric radar data. A need exists for a user-friendly, interactive software package for analysis of these polarimetric radar data sets. Previous software packages, such as JPL's Multiview, while providing some analysis capabilities for these data, did not allow interactive viewing and were tied to specific image display hardware with operating system dependencies. A prototype software system, the 'Radar Analysis and Visualization Environment' (RAVEN) under development at the Center for the Study of Earth from Space (CSES) at the University of Colorado, is designed to deal with data from the JPL AIRSAR instrument and other proposed polarimetric radar instruments. RAVEN is being developed using the Interactive Data Language (IDL). It takes advantage of high speed disk access and fast processors running under the UNIX operating system in an X-windows environment to allow for rapid, interactive visualization of AIRSAR data in both image and graphical ways. It provides a user-friendly interface through the use of menus, sliders, buttons, and display windows.

Kierein-Young, K. S.↗

Application of Quasi-Static Modal Analysis to an Orion Multi-Purpose Crew Vehicle Test

Bolted structural joints often exhibit load-dependent stiffness and energy dissipation that leads to nonlinear, amplitude dependent frequency and damping in the structure. As an alternative to direct integration of the nonlinear equations of motion, quasi-static modal analysis (QSMA) determines the dependence of frequency and damping on response amplitude using loading behavior from nonlinear static analyses. QSMA has previously been demonstrated to substantially reduce computational cost and maintain accuracy relative to full nonlinear dynamic simulation. This work explores the applicability of QSMA to a complex, large-scale aerospace structure. QSMA is employed to analyze a nonlinear model of test hardware developed to support the Orion Multi-Purpose Crew Vehicle program, which exhibited nonlinear behavior during dynamic testing at flight-like load levels. In addition to the extraction of amplitude-dependent frequency and damping curves, a Bouc-Wen hysteresis model was used in conjunction with the quasi-static results to develop nonlinear, uncoupled, time-domain modal equations of motion for the structure. Excellent agreement was observed between the reduced and full-order nonlinear models, encouraging future employment of QSMA to support accurate and efficient model reduction of structures with bolted joint nonlinearities.

QSMA↗

Computational Analysis for Roughness-Based Transition Control

Suitably placed discrete roughness elements are known to delay or hasten the onset of transition, depending on requirements. In this paper, 2D eigenvalue analysis is used to study the effects of surface roughness in the context of transition delay over subsonic and supersonic swept wing configurations, as well as boundarylayer tripping on the forebody of a hypersonic air breathing vehicle.

Li, Fei↗

Methodology for object-oriented real-time systems analysis and design: Software engineering

Successful application of software engineering methodologies requires an integrated analysis and design life-cycle in which the various phases flow smoothly 'seamlessly' from analysis through design to implementation. Furthermore, different analysis methodologies often lead to different structuring of the system so that the transition from analysis to design may be awkward depending on the design methodology to be used. This is especially important when object-oriented programming is to be used for implementation when the original specification and perhaps high-level design is non-object oriented. Two approaches to real-time systems analysis which can lead to an object-oriented design are contrasted: (1) modeling the system using structured analysis with real-time extensions which emphasizes data and control flows followed by the abstraction of objects where the operations or methods of the objects correspond to processes in the data flow diagrams and then design in terms of these objects; and (2) modeling the system from the beginning as a set of naturally occurring concurrent entities (objects) each having its own time-behavior defined by a set of states and state-transition rules and seamlessly transforming the analysis models into high-level design models. A new concept of a 'real-time systems-analysis object' is introduced and becomes the basic building block of a series of seamlessly-connected models which progress from the object-oriented real-time systems analysis and design system analysis logical models through the physical architectural models and the high-level design stages. The methodology is appropriate to the overall specification including hardware and software modules. In software modules, the systems analysis objects are transformed into software objects.

Schoeffler, James D.↗

Materials analysis by ultrasonics: Metals, ceramics, composites

Research results in analytical ultrasonics for characterizing structural materials from metals and ceramics to composites are presented. General topics covered by the conference included: status and advances in analytical ultrasonics for characterizing material microstructures and mechanical properties; status and prospects for ultrasonic measurements of microdamage, degradation, and underlying morphological factors; status and problems in precision measurements of frequency-dependent velocity and attenuation for materials analysis; procedures and requirements for automated, digital signal acquisition, processing, analysis, and interpretation; incentives for analytical ultrasonics in materials research and materials processing, testing, and inspection; and examples of progress in ultrasonics for interrelating microstructure, mechanical properties, and dynamic response.

Vary, Alex↗