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At least 829 records · Page 46
All sourcesNASA NTRSDOE OSTI
NASA NTRS2014

Effects of Radiation and Dietary Iron on Expression of Genes and Proteins Involved in Drug Metabolism

Liver function, especially the rate of metabolic enzyme activities, determines the concentration of circulating drugs and the duration of their efficacy. Most pharmaceuticals are metabolized by the liver, and clinically-used medication doses are given with normal liver function in mind. A drug overdose can result in the case of a liver that is damaged and removing pharmaceuticals from the circulation at a rate slower than normal. Alternatively, if liver function is elevated and removing drugs from the system more quickly than usual, it would be as if too little drug had been given for effective treatment. Because of the importance of the liver in drug metabolism, we want to understand any effects of spaceflight on the enzymes of the liver. Dietary factors and exposure to radiation are aspects of spaceflight that are potential oxidative stressors and both can be modeled in ground experiments. In this experiment, we examined the effects of high dietary iron and low dose gamma radiation (individually and combined) on the gene expression of enzymes involved in drug metabolism, redox homeostasis, and DNA repair. METHODS All procedures were approved by the JSC Animal Care and Use Committee. Male Sprague-Dawley rats were divided into 4 groups (n=8); control, high Fe diet (650 mg iron/kg), radiation (fractionated 3 Gy exposure from a Cs- 137 source) and combined high Fe diet + radiation exposure. Animals were euthanized 24h after the last treatment of radiation; livers were removed immediately and flash -frozen in liquid nitrogen. Expression of genes thought to be involved in redox homeostasis, drug metabolism and DNA damage repair was measured by RT-qPCR. Where possible, protein expression of the same genes was measured by western blotting. All data are expressed as % change in expression normalized to reference gene expression; comparisons were then made of each treatment group to the sham exposed/ normal diet control group. Data was considered significant at p< 0.5. RESULTS Among the redox homeostasis genes examined, metallothionein showed a significant down regulation in the radiation treated group (-3.85 fold) and a trend toward down regulation in the high Fe + rad group. Metallothionein is involved in the regulation of physiological metals and also has antioxidant activities. Among the drug metabolism genes examined, ATP binding cassette subfamily B (Abcb1b) gene expression increased more than 10-fold in both groups that received radiation treatments. This increased expression was also seen at the protein level. This ABC transporter carries many different compounds across cell membranes, including administered medications. The cytochrome P450 2E1 enzyme, a mixed-function oxidase that deactivates some medications and activates others, showed about a 2-fold increase in gene expression in both radiation-treated groups, with a trend toward increased expression at the protein level. Expression of epoxide hydrolase, which detoxifies polycyclic aromatic hydrocarbons, showed similar 2-fold increases. Among the DNA repair genes examined, expression of RAD51 was significantly down regulated (1.5 fold) in the radiation treated group. RAD51 is involved in repair of double-stranded DNA breaks. CONCLUSION This experiment used 2 different sources of physiological oxidative stress, administered separately and together, and examined their impacts on liver gene and protein expression. It is clear that significant changes occurred in expression of several genes and proteins in the radiation-treated animals. If the results from this ground analog of portions of the spaceflight environment hold true for the spaceflight environment itself, the physiological roles of the affected enzymes (drug transport and metabolism, redox homeostasis) could mean consequences in redox homeostasis or the pharmacokinetics of administered medications

Faust, K. M.↗
NASA NTRS1981

LANDSAT applications by the Adirondack Park Agency for land cover analyses and forest cover change

The New York State Adirondack Park Agency is using LANDSAT imagery to provide current, consistent parkwide data on forest cover, forest change and other land cover characteristics for the Adirondack Park, an area of 9,375 sq. miles (24,280 sq km). Boundaries of the study area were digitized and the data were enhanced and geographically rectified. A classification scheme was devised which emphasized the basic land cover types of the Park: hardwoods, spruce-fir, pine, wet conifer, brushland, grassland, agricultural areas, exposed earth, urban areas, and water bodies. Cover type classifications for disturbed forest land were also chosen: cut hardwoods, regenerating hardwoods, and cut spruce fir. Field verification of 1978 classification revealed an accurate differentiation of forest types within types and between nonforested/forested areas. The classification accurately detects forest land disturbances; however, it is not always descriptive of the level of disturbance.

Banta, J. S.↗
NASA NTRS1986

Coupled stratospheric ozone and temperature responses to short-term changes in solar ultraviolet flux - An analysis of Nimbus 7 SBUV and SAMS data

Earlier studies of solar-induced variations in stratospheric parameters have been mainly concerned with observed ozone responses. In the present investigation, attention is given to temperature responses as well as ozone responses at low latitudes, taking into account 22 months of Nimbus 7 solar backscattered ultraviolet (SBUV) ozone and stratospheric and mesospheric sounder temperature data. A data description is provided, and cross-correlation and regression analyses are conducted. An extension is considered of an analytic model, which was derived by Frederick (1981) for the coupled behavior of ozone and temperature perturbations in the upper stratosphere and lower mesosphere. The extended model is applied to the results of the analyses. The obtained data provide statistical evidence for ozone and temperature responses to changes in solar ultraviolet flux on the time scale of the solar rotation period.

Hood, L. L.↗
NASA NTRS2002

Geostatistical Methods For Determination of Roughness, Topography, And Changes of Antarctic Ice Streams From SAR And Radar Altimeter Data

The central objective of this project has been the development of geostatistical methods fro mapping elevation and ice surface characteristics from satellite radar altimeter (RA) and Syntheitc Aperture Radar (SAR) data. The main results are an Atlas of elevation maps of Antarctica, from GEOSAT RA data and an Atlas from ERS-1 RA data, including a total of about 200 maps with 3 km grid resolution. Maps and digital terrain models are applied to monitor and study changes in Antarctic ice streams and glaciers, including Lambert Glacier/Amery Ice Shelf, Mertz and Ninnis Glaciers, Jutulstraumen Glacier, Fimbul Ice Shelf, Slessor Glacier, Williamson Glacier and others.

Herzfeld, Ute C.↗
NASA NTRS2012

Landsat Data Continuity Mission (LDCM) Ascent and Operational Orbit Design

For the past 40-years, Landsat Satellites have collected Earth's continental data and enabled scientists to assess change in the Earth's landscape. The Landsat Data Continuity Mission (LDCM) is the next generation satellite supporting the Landsat science program. LDCM will fly a 16-day ground repeat cycle, Sun-synchronous, frozen orbit with a mean local time of the descending node ranging between 10:10 am and 10:15 am. This paper presents the preliminary ascent trajectory design from the injection orbit to its final operational orbit. The initial four burn ascent design is shown to satisfy all the LDCM mission goals and requirement and to allow for adequate flexibility in re-planning the ascent.

Mann, Laurie M.↗
NASA NTRS1973

Simulator performance validation and improvement through recorded data.

During the development of two-segment approach procedures instrumentation systems were installed on a 727 flight simulator to monitor a variety of flight parameters. Parameters monitored and data format were designed to be the same as those to be used in later flight tests with line aircraft. Good correlation existed between simulator and aircraft data in many areas. Previously identified areas of poor simulation were checked with quantitative data. The objective data obtained could be used for simulation improvements in areas previously changed primarily by using subjective data obtained from pilots. Areas where similar techniques could improve simulation were identified.

Seay, D. L.↗
NASA NTRS1990

The EOS data and information system

The Earth Observing System (EOS) is proposed as a 1991 new initiative by NASA as part of the Mission to Planet Earth. One of the key components of the EOS program is the EOS Data and Information System (EOSDIS). Two independent Phase B studies of EOSDIS were conducted from January 1989 through April 1990. Some of the key challenges faced by EOSDIS are: satisfying the data and information needs of a diverse multidisciplinary scientific community integrating product generation algorithms for over two dozen instruments, keeping up with an orbital average data rate of over 50 Mb/sec and assuring prompt generation of standard products, reprocessing data as product generation algorithms change, and storing, and managing information about tens of Petabytes of data over the 15-year life of the mission.

Ramapriyan, H. K.↗
NASA NTRSDate not supplied

TPSAS-NF1676L-35172-DND

The wide field of view instrument on Earth Radiation Budget Experiment (ERBE) Earth Radiation Budget Satellite (ERBS) took measurements from November 1984 through September 1999. It provides top-of-atmosphere (TOA) reflected shortwave and emitted longwave irradiance at a 10 degree by 10 degree spatial resolution. We have recently re-processed ERBS wide field of view data with algorithms similar to those used in the CERES process (Shrestha et al. 2019). We also calibrated ERBS shortwave and longwave irradiance with CERES-derived irradiances. Combining ERBS and CERES data, we analyze the time series of global mean net TOA irradiance from 1985. The change of TOA net radiation over 30 years derived from ERBS and CERES data is larger than the change derived by Allan et al. (2014). The difference in the net irradiance change from two data sets is, however, within the uncertainty of ocean heating rates derived from ocean temperature measurements in 80?s. We also investigate the spatial distribution of changes in reflected shortwave irradiances derived from ERBS and CERES periods. The spatial distribution of reflected shortwave irradiance changes over the tropics and sub-tropical regions derived from our data set is qualitatively consistent with the result of Norris et al. (2016).

Antonio Viudez-Mora↗
NASA NTRS1984

Tortuosity of lightning return stroke channels

Data obtained from photographs of lightning are presented on the tortuosity of return stroke channels. The data were obtained by making piecewise linear fits to the channels, and recording the cartesian coordinates of the ends of each linear segment. The mean change between ends of the segments was nearly zero in the horizontal direction and was about eight meters in the vertical direction. Histograms of these changes are presented. These data were used to create model lightning channels and to predict the electric fields radiated during return strokes. This was done using a computer generated random walk in which linear segments were placed end-to-end to form a piecewise linear representation of the channel. The computer selected random numbers for the ends of the segments assuming a normal distribution with the measured statistics. Once the channels were simulated, the electric fields radiated during a return stroke were predicted using a transmission line model on each segment. It was found that realistic channels are obtained with this procedure, but only if the model includes two scales of tortuosity: fine scale irregularities corresponding to the local channel tortuosity which are superimposed on large scale horizontal drifts. The two scales of tortuosity are also necessary to obtain agreement between the electric fields computed mathematically from the simulated channels and the electric fields radiated from real return strokes. Without large scale drifts, the computed electric fields do not have the undulations characteristics of the data.

Levine, D. M.↗
NASA NTRS1988

A regional perspective on the palynofloral response to K-T boundary event(s) with emphasis on variations imposed by the effects of sedimentary facies and latitude

Palynological studies deal with fossil reproductive bodies that were produced by fully functioning plants, whereas most faunal studies are based on death assemblages. Therefore, changes in pollen and spore assemblages cannot be used directly as evidence of catastrophic mass killings but only to indicate changes in ecological conditions. The impact of the Cretaceous-Tertiary boundary event on terrestrial plant communities is illustrated by the degree, rate and selectivity of change. As in most classical palynological studies, the degree of change is expressed in terms of relative abundance and changes in species diversity. It is recognized that sampling interval and continuity of the rock record within individual sections can affect the percieved rate of change. Even taking these factors into account, a gradual change in relative abundance and multiple levels of apparent extinctions, associated with the interval bounding the K-T boundary, can be demonstrated. Climatic change, which locally exceeds the tolerance of individual species, and the possible loss of a group of pollinating agents are examined as possible explanations for the selectivity of apparent extinctions and/or locally truncated occurrences. The aspects of change are demonstrated with data from four different K-T boundary localities in Western Canada between paleolatitudes 60 and 75 deg north. Together, the four localities discussed allow changes imposed by latitude and differences in the depositional environment be isolated from the boundary event itself which is reflected by the truncated ranges of several species throughout the region of study. What must be recognized is that variations in the response of vegetation to the K-T boundary event(s) occurred throughout the Western Interior basin.

Sweet, A. R.↗
NASA NTRS1973

Land use classification and change analysis using ERTS-1 imagery in CARETS

Land use detail in the CARETS area obtainable from ERTS exceeds the expectations of the Interagency Steering Committee and the USGS proposed standardized classification, which presents Level 1 categories for ERTS and Level 2 for high altitude aircraft data. Some Levels 2 and 3, in addition to Level 1, categories were identified on ERTS data. Significant land use changes totaling 39.2 sq km in the Norfolk-Portsmouth SMSA were identified and mapped at Level 2 detail using a combination of procedures employing ERTS and high altitude aircraft data.

Alexander, R. H.↗
NASA NTRS2000

BOREAS TGB-12 Rn-222 Activity Data over the NSA

The BOREAS TGB-12 team made measurements of soil carbon inventories, carbon concentration in soil gases, and rates of soil respiration at several sites to estimate the rates of carbon accumulation and turnover in each of the major vegetation types. Sampling strategies for soils were designed to take advantage of local fire chronosequences, so that the accumulation of carbon in regrowing mosses could be determined. All the data are used to: (1) calculate the inventory of carbon and nitrogen in moss and mineral soil layers at NSA sites, (2) determine the rates of input and turnover (using both accumulation since the last stand-killing fire and radiocarbon data), and (3) link changes in soil respiration rate to shifts in the C-14 content of soil CO2 to determine the average "age" respired CO2 . These Rn-222 activity data were collected from 15-Nov-1993 to 16-Aug-1994 over the NSA sites. They are useful for determining the rate of gas exchange between soil and the overlying atmosphere. The data in this data set are stored in tabular ASCII files.

Trumbore, Susan↗
NASA NTRS2000

BOREAS TGB-12 Rn-222 Flux Data over the NSA

The BOREAS TGB-12 team made measurements of soil carbon inventories, carbon concentration in soil gases, and rates of soil respiration at several sites to estimate the rates of carbon accumulation and turnover in each of the major vegetation types. Sampling strategies for soils were designed to take advantage of local fire chronosequences, so that the accumulation of carbon in new moss growth could be determined. All the data are used to 1) calculate the inventory of carbon and nitrogen in moss and mineral soil layers at NSA sites, 2) determine the rates of input and turnover (using both accumulation since the last stand-killing fire and radiocarbon data), and 3) link changes in soil respiration rate to shifts in the C-14 content of soil CO2 to determine the average "age" respired CO2. These Rn-222 flux data were collected from 15-Nov-1993 to 16-Aug-1994 over the NSA sites. The data in this data set are stored in tabular ASCII files.

Hall, Forrest G.↗
NASA NTRS2004

An Experimental Study Synthetic Jets from Rectangular Orifices

Results of an experimental investigation on isolated synthetic jets in crossflow from rectangular orifices of different aspect ratio and orientation are presented. Three aspect ratios, AR = 4, 8, and 16, with pitch a = 90deg., were investigated, Additionally, the AR = 8 case was pitched at 20 deg.. The yaw angle, , was varied through 0 deg., 10 deg., 45 deg. and 90 deg.. All orifices had same exit area and the data were compared with synthetic as well as steady jet from a circular orifice of same area. Hotwire measurements were performed to obtain all three components of mean velocity and turbulent stresses. Data were acquired for momentum-flux ratio up to J = 50. Distributions of time- and phase-averaged data were obtained on the cross sectional plane at x/D = 0.5, 5 and 10, as well as on the axial plane of the symmetry. Qualitative flowfield similarity between synthetic and steady jets is observed. However, high-momentum 'cap' above the low-momentum 'dome', characteristic of steady jets, does not necessarily appear in the synthetic jet. The position and shape of the high-momentum region depend on the distance from the orifice, pitch, yaw as well as momentum-flux ratio. Consequently, the location of the minimum velocity in the 'dome' measured at the plane of symmetry, y(submin), is adopted as a reference for penetration estimate and trajectory comparison. For AR = 16, the dome is the largest in area with maximum velocity deficit. However, the penetration is somewhat higher for AR = 4. Increase in yaw reduces the spatial extent of the dome and the penetration height but augments the velocity deficit. At low J the dome is connected to the boundary layer and traces of the cap of high momentum fluid are visible above it. Increase in J lifts the dome and reorganizes the high-momentum fluid around its perimeter, eventually bringing it underneath. Phase-averaged data document dynamic topological changes within the cycle. Phase-averaged streamwise velocity contours on the cross-sectional plane exhibit behavior commensurate with that seen in time-averaged data at various J.

Milanovic, Ivana M.↗
NASA NTRS2019

Deterministic Polishing of Replicating Grazing-Incidence Mandrels

In an effort to manufacture high-angular-resolution, grazing-incidence, x-ray optics, Marshall Space Flight Center (MSFC) is taking measures to improve its electroformed replicated optics. A key development is the use of computer-numerical control (CNC) polishing to deterministically improve the surface of electroless nickel mandrels used to replicate grazing- incidence optics. Metrology, control software and polishing parameters must function together seamlessly to reach the specifications required to replicate sub-arcsecond optics. Each change in polishing parameters effects the wear pattern of the polishing head. Using Richardson-Lucy deconvolution, the controller software fits the wear pattern to metrology data to calculate the changing feedrates across the mandrel. Here we present an overview of our process, and early results showing the effectiveness of deterministic polishing for replicated optics.

Davis, Jacqueline↗
NASA NTRS2020

The NASA Micro Pulse Lidar Network (MPLNET): Introduction of the New Version 3 Release

The NASA Micro-Pulse Lidar Network (MPLNET) is a global federated network of polarized Micro-Pulse Lidar (MPL) systems running continuously. MPLNET began in 2000, and there have been over 70 sites deployed worldwide, with 24 sites currently active and a few more planned over the next year. Seven of the long-term sites have 10+ years of data, and many more have 5+ years. Most sites are co-located with AERONET providing joint data on column and vertically resolved aerosol and cloud information. This presentation will introduce our new Version 3 MPLNET data. All sites in the network now feature eye-safe polarized backscatter MPL instruments, providing information on attenuated backscatter and particle shape. In addition to change with our signal data, we have an enhanced cloud product suite, a new PBL height product, and inclusion of the new AERONET lunar aerosol optical depth into MPLNET aerosol retrievals. A new quality flag process will be used to better describe all data products. Finally, a new data portal will provide near-real-time (NRT) access to all data products, including new quality assured NRT L1.5 products. Custom products developed for model specific applications will also be provided.

Welton, Ellsworth J.↗
NASA NTRS2015

Decision Making and Communications Process Assessment of NASA Using Three Change Requests from the Space Launch System Program

This thesis investigated the communication and decision making process as part of the Systems Engineering practices at the NASA/Marshall Center to determine its level of effectiveness. Data was collected across three change requests to assess how decisions were made, how the decisions were communicated, and whether a process mattered in the formulation and dissemination of those decisions. Data results revealed the comprehensive decision making process for the technical change requests to be effective. Evidence revealed that the process was sufficiently tailored to accommodate the need of each individual technical change which promoted effective communication amongst the stakeholders in the formulation of the strategic decision recommendations elevated to upper management. However, data results also revealed the dissemination of the final decision and approval of the change requests from the higher organizational level down to all stakeholders was less effective. An establishment of a culmination meeting at the end of the change request decision process in which to close the communication loop with all entities would be beneficial.

Hicks, Karen Campbell↗
NASA NTRS1990

Discrepancies in the earth-atmosphere angular momentum budget

Modern geodetic and atmospheric data sets have demonstrated that changes in the solid earth's rotation rate are closely coupled to changes in the atmosphere's angular momentum over a range of subseasonal through interannual time scales. Nevertheless, limits to the correspondence between changes in the momenta of the two bodies exist at both ends of the spectrum resolvable by the data. It is shown that, at high frequencies, changes in the length of day as short as a fortnight can now be attributed almost entirely to atmospheric forcing; more rapid fluctuations in the length of day, however, cannot be shown to coincide with atmospheric behavior, a result blamed mostly on errors remaining in both the geodetic and atmospheric series rather than on the neglect of some other component of the momentum budget. On the decadal time scale, nonatmospheric processes, believed primarily to involve core-mantle coupling, dominate the global momentum budget.

Rosen, Richard D.↗