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At least 829 records · Page 46

Genetic separation of phototropism and blue light inhibition of stem elongation

Blue light-induced regulation of cell elongation is a component of the signal response pathway for both phototropic curvature and inhibition of stem elongation in higher plants. To determine if blue light regulates cell elongation in these responses through shared or discrete pathways, phototropism and hypocotyl elongation were investigated in several blue light response mutants in Arabidopsis thaliana. Specifically, the blu mutants that lack blue light-dependent inhibition of hypocotyl elongation were found to exhibit a normal phototropic response. In contrast, a phototropic null mutant (JK218) and a mutant that has a 20- to 30-fold shift in the fluence dependence for first positive phototropism (JK224) showed normal inhibition of hypocotyl elongation in blue light. F1 progeny of crosses between the blu mutants and JK218 showed normal phototropism and inhibition of hypocotyl elongation, and approximately 1 in 16 F2 progeny were double mutants lacking both responses. Thus, blue light-dependent inhibition of hypocotyl elongation and phototropism operate through at least some genetically distinct components.

Non-NASA Center↗

DVB-S2 Experiment over NASA's Space Network

The commercial DVB-S2 standard was successfully demonstrated over NASAs Space Network (SN) and the Tracking Data and Relay Satellite System (TDRSS) during testing conducted September 20-22nd, 2016. This test was a joint effort between NASA Glenn Research Center (GRC) and Goddard Space Flight Center (GSFC) to evaluate the performance of DVB-S2 as an alternative to traditional NASA SN waveforms. Two distinct sets of tests were conducted: one was sourced from the Space Communication and Navigation (SCaN) Testbed, an external payload on the International Space Station, and the other was sourced from GRCs S-band ground station to emulate a Space Network user through TDRSS. In both cases, a commercial off-the-shelf (COTS) receiver made by Newtec was used to receive the signal at White Sands Complex. Using SCaN Testbed, peak data rates of 5.7 Mbps were demonstrated. Peak data rates of 33 Mbps were demonstrated over the GRC S-band ground station through a 10MHz channel over TDRSS, using 32-amplitude phase shift keying (APSK) and a rate 89 low density parity check (LDPC) code. Advanced features of the DVB-S2 standard were evaluated, including variable and adaptive coding and modulation (VCMACM), as well as an adaptive digital pre-distortion (DPD) algorithm. These features provided additional data throughput and increased link performance reliability. This testing has shown that commercial standards are a viable, low-cost alternative for future Space Network users.

waveforms↗

Electromagnetic scattering from two identical pseudospheres

In an attempt to gain some insight into the problem of how close particles have to be on the average before phase effects become important, the cross section and phase matrices for two identical particles as a function of the distance between their centers for both broadside and end-on illumination have been calculated. The point dipole approximation is employed to build two pseudospherical particles each consisting of 32 dipoles with an effective size parameter of 0.9283 and refractive index of 1.54-0.0i. In regard to cross sections, it is found that in certain cases several percent deviation from the converged value, which is twice the single particle cross section, could be found up to distances of separation of 25 single particle diameters. The maxima and minima in the cross section curves can be explained in terms of simple interference effects. Also the element P11 of the phase matrix showed a great deal of structure, and the position of maxima and minima could be explained by phase shift analysis.

Kattawar, G. W.↗

Experimental Demonstration of Microwave Signal/Electric Thruster Plasma Interaction Effects

An experiment was designed and conducted in the Electric Propulsion Laboratory of NASA Lewis Research Center to assess the impact of ion thruster exhaust plasma plume on electromagnetic signal propagation. A microwave transmission experiment was set up inside the propulsion test bed using a pair of broadband horn antennas and a 30 cm 2.3 kW ion thruster. Frequency of signal propagation covered from 6.5 to 18 GHz range. The stainless steel test bed when enclosed can be depressurized to simulate a near vacuum environment. A pulsed CW system with gating hardware was utilized to eliminate multiple chamber reflections from the test signal. Microwave signal was transmitted and received between the two hours when the thruster was operating at a given power level in such a way that the signal propagation path crossed directly through the plume volume. Signal attenuation and phase shift due to the plume was measured for the entire frequency band. Results for this worst case configuration simulation indicate that the effects of the ion thruster plume on microwave signals is a negligible attenuation (within 0.15 dB) and a small phase shift (within 8 deg.). This paper describes the detailed experiment and presents some of the results.

Zaman, Afroz J.↗

Direct Detection Doppler Lidar for Spaceborne Wind Measurement

Aerosol and molecular based versions of the double-edge technique can be used for direct detection Doppler lidar spaceborne wind measurement. The edge technique utilizes the edge of a high spectral resolution filter for high accuracy wind measurement using direct detection lidar. The signal is split between an edge filter channel and a broadband energy monitor channel. The energy monitor channel is used for signal normalization. The edge measurement is made as a differential frequency measurement between the outgoing laser signal and the atmospheric backscattered return for each pulse. As a result the measurement is insensitive to laser and edge filter frequency jitter and drift at a level less than a few parts in 10(exp 10). We have developed double edge versions of the edge technique for aerosol and molecular-based lidar measurement of the wind. Aerosol-based wind measurements have been made at Goddard Space Flight Center and molecular-based wind measurements at the University of Geneva. We have demonstrated atmospheric measurements using these techniques for altitudes from 1 to more than 10 km. Measurement accuracies of better than 1.25 m/s have been obtained with integration times from 5 to 30 seconds. The measurements can be scaled to space and agree, within a factor of two, with satellite-based simulations of performance based on Poisson statistics. The theory of the double edge aerosol technique is described by a generalized formulation which substantially extends the capabilities of the edge technique. It uses two edges with opposite slopes located about the laser frequency at approximately the half-width of each edge filter. This doubles the signal change for a given Doppler shift and yields a factor of 1.6 improvement in the measurement accuracy compared to the single edge technique. The use of two high resolution edge filters substantially reduces the effects of Rayleigh scattering on the measurement, as much as order of magnitude, and allows the signal to noise ratio to be substantially improved in areas of low aerosol backscatter. We describe a method that allows the Rayleigh and aerosol components of the signal to be independently determined using the two edge channels and an energy monitor channel. The effects of Rayleigh scattering may then subtracted from the measurement and we show that the correction process does not significantly increase the measurement noise for Rayleigh to aerosol ratios up to 10. We show that for small Doppler shifts a measurement accuracy of 0.4 m/s can be obtained for 5000 detected photon, 1.2 m/s for 1000 detected photons, and 3.7 m/s for 50 detected photons for a Rayleigh to aerosol ratio of 5. Methods for increasing the dynamic range of the aerosol-based system to more than +/- 100 m/s are given.

Korb, C. Laurence↗

The VISAR Process

The Video Image Stabilization And Registration (VISAR) process is an award winning video image processing software developed at NASA's Marshall Space Flight Center. VISAR has a wide variety of application areas where the refinement of digital video is needed. It is used to correct jitter, rotation, and zoom effects by registering and processing on individual image captures that are a part of normal video capturing. Its most prominent uses were the 1996 Olympic Bombing case and in identifying Saddam Hussein during the Iraq war. Based on first-hand knowledge, this paper describes the VISAR process, which consists of several steps designed to refine digital video using VISAR software. The process determines the differences between two video images so that one, or both, of the images can be changed in ways that make them match as well as possible. Corrections include changes in position (horizontal and vertical image shifts), changes in orientation (image rotation), and changes in magnification (image zoom). While much of the VISAR process is automated, in its current embodiment it requires the user to initially identify the area of interest and to reset a threshold parameter if the default gives unacceptable results. The basic process that is used is an old tried and true method that determines how well the two images match. This process is called cross-correlation. It gives a single number, the correlation coefficient, that is equal to 1.0 if the images are perfectly matched, is equal to 0.0 if the images have nothing in common, and is equal to -1.0 if one image is the negative of the other. This basic process is used by many image stabilization methods. With VISAR we use it in a manner that provides statistical information needed to best determine orientation and magnification.

Hathaway, David↗

The evolution of Crew Resource Management training in commercial aviation

In this study, we describe changes in the nature of Crew Resource Management (CRM) training in commercial aviation, including its shift from cockpit to crew resource management. Validation of the impact of CRM is discussed. Limitations of CRM, including lack of cross-cultural generality are considered. An overarching framework that stresses error management to increase acceptance of CRM concepts is presented. The error management approach defines behavioral strategies taught in CRM as error countermeasures that are employed to avoid error, to trap errors committed, and to mitigate the consequences of error.

Non-NASA Center↗

Early wrong-way response occurs in orthogravitropism of maize roots treated with lithium

Application of lithium ions to tips of roots of Zea mays L. cv. Silver Queen shifts the direction of initial orthogravitropic curvature from downward to upward. The production of this putatively incidental perturbation of orthogravitropic bending kinetics by a pharmacological agent might provide insight into both ortho- and plagiogravitropism. Additionally, the protocol of the experiments bears on recent claims that mucilage external to the root cap plays an essential role in gravitropism. External mucilage was removed before roots were stimulated, yet they reached about 50 degrees gravitropic curvature in an hour.

Non-NASA Center↗

Rotating shift work, sleep, and accidents related to sleepiness in hospital nurses

A hospital-based survey on shift work, sleep, and accidents was carried out among 635 Massachusetts nurses. In comparison to nurses who worked only day/evening shifts, rotators had more sleep/wake cycle disruption and nodded off more at work. Rotators had twice the odds of nodding off while driving to or from work and twice the odds of a reported accident or error related to sleepiness. Application of circadian principles to the design of hospital work schedules may result in improved health and safety for nurses and patients.

Non-NASA Center↗

BOREAS RSS-17 1994 ERS-1 Level-3 Freeze/Thaw Backscatter Change Images

The Boreal Ecosystem-Atmosphere Study (BOREAS) Remote Sensing Science (RSS)-17 team acquired and analyzed imaging radar data from the European Space Agency's (ESA's) European Remote Sensing Satellite (ERS)-1 over a complete annual cycle at the BOREAS sites in Canada in 1994 to detect shifts in radar backscatter related to varying environmental conditions. Two independent transitions corresponding to soil thaw and possible canopy thaw were revealed by the data. The results demonstrated that radar provides an ability to observe thaw transitions at the beginning of the growing season, which in turn helps constrain the length of the growing season. The data set presented here includes change maps derived from radar backscatter images that were mosaicked together to cover the southern BOREAS sites. The image values used for calculating the changes are given relative to the reference mosaic image. The data are stored in binary image format files. The imaging radar data are available from the Earth Observing System Data and Information System (EOSDIS) Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC). The data files are available on a CD-ROM (see document number 20010000884).

Rignot, Eric↗

Statistical Process Control and Capability Study on the Water Content Measurements in NASA Glenn's Icing Research Tunnel

The Icing Research Tunnel (IRT) at NASA Glenn Research Center follows the recommended practice for icing tunnel calibration outlined in SAE's ARP5905 document. The calibration team has followed the schedule of a full calibration every five years with a check calibration done every six months following. The liquid water content of the IRT has maintained stability within in the specifications presented to customers that the variation is within +/- 10% of the calibrated, target measurement. With recent measurements and instrumentation errors, a more thorough assessment of error source was desired. By constructing statistical process control charts, the ability to determine how the instrument varies in the short term, mid term, and long term was gained. The control charts offer a view of instrument error, facility error, or installation changes. It was discovered that there was a shift from target to mean baseline thus leading to the study of the overall capability indices of the liquid water content measuring instrument to perform within specifications defined in the IRT. This presentation describes data processing procedures for the Multi-Element Sensor in the IRT, including collision efficiency corrections, canonical correlation analysis, Chauvenet's Criterion for rejection of data, distribution check of data, and mean, median and mode for construction of control charts. Further data is presented to describe the repeatability of the IRT with the Multi-Element Sensor and the ability to maintain a stable process for the defined calibration schedule.

Timko, Emily N.↗

The effects of hindlimb unweighting on the capacitance of rat small mesenteric veins

Microgravity is associated with an impaired cardiac output response to orthostatic stress. Mesenteric veins are critical in modulating cardiac filling through venoconstriction. The purpose of this study was to determine the effects of simulated microgravity on the capacitance of rat mesenteric small veins. We constructed pressure-diameter relationships from vessels of 21-day hindlimb-unweighted (HLU) rats and control rats by changing the internal pressure and measuring the external diameter. Pressure-diameter relationships were obtained both before and after stimulation with norepinephrine (NE). The pressure-diameter curves of HLU vessels were shifted to larger diameters than control vessels. NE (10(-4) M) constricted veins from control animals such that the pressure-diameter relationship was significantly shifted downward (i.e., to smaller diameters at equal pressure). NE had no effect on vessels from HLU animals. These results indicate that, after HLU, unstressed vascular volume may be increased and can no longer decrease in response to sympathetic stimulation. This may partially underlie the mechanism leading to the exaggerated fall in cardiac output and stroke volume seen in astronauts during an orthostatic stress after exposure to microgravity.

Non-NASA Center↗

Improving Scientific Capabilities in Space in the 21st Century: The NASA New Millennium Program

NASA's New Millennium Program (NMP) has been chartered to identify and validate in space emerging, revolutionary technologies that will enable less costly, more capable future science missions. The program utilizes a unique blend of science guidance and industry partnering to ferret out technology solutions to enable science capabilities in space which are presently technically infeasible, or unaffordable. Those technologies which present an unacceptably high risk to future science missions (whether small PI-led or operational) are bundled into technology validation missions. These missions seek to validate the technologies in a manner consistent with their future uses, thus reducing the associated risk to the first user, and obtaining meaningful science data as well. The Space Readiness Coherent Lidar Experiment (SPARCLE) was approved as the second NMP Earth Observing mission (EO2) in October 1997, and assigned to Marshall Space Flight Center for implementation. Leading up to mission confirmation, NMP sponsored a community workshop in March 1996 to draft Level-1 requirements for a doppler wind lidar mission, as well as other space-based lidar missions (such as DIAL). Subsequently, a study group was formed and met twice to make recommendations on how to perform a comparison of coherent and direct detection wind lidars in space. These recommendations have guided the science validation plan for the SPARCLE mission, and will ensure that future users will be able to confidently assess the risk profile of future doppler wind missions utilizing EO2 technologies. The primary risks to be retired are: (1) Maintenance of optical alignments through launch and operations on orbit, and (2) Successful velocity estimation compensation for the Doppler shift due to the platform motion, and due to the earth's rotation. This includes the need to account for all sources of error associated with pointing control and knowledge. The validation objectives are: (1) Demonstrate measurement of tropospheric winds from space using a scanning coherent Doppler lidar technique that scales to meet future research (e.g. ESSP) and operational (e.g. NPOESS) mission requirements. Specifically, produce and validate LOS wind data with single shot accuracy of 1-2 m/s in regions of high signal-to-noise ratio (SNR), and low atmospheric wind turbulence and wind shear, (2) Collect the atmospheric and instrument performance data in various scanning modes necessary to validate and improve instrument performance models that will enable the definition of future missions with greater confidence. Such data include aerosol backscatter data over much of the globe, and high SNR data such as that from surface returns, and (3) Produce a set of raw instrument data with which advanced signal processing techniques can be developed. This objective will permit future missions to better understand how to extract wind information from low backscatter regions of the atmosphere.

Raymond, Carol A.↗

Hybridized Agile Software Development of Flight Control Team Tools for International Space Station's Payload Operations Integration Center

Ground systems operations at the National Aeronautics and Space Administration's (NASA) Payload Operations and Integration Function (POIF) is increasing, via the High Operations Tempo (HOT) initiative, its ability to support more science activities with a fourth crew member on the International Space Station (ISS). The Flight Control Team's need to support this increased crew presence mandated the need for a series of software tools to better handle the increasing pace of payload science operations. The overall need was clear from the outset, but the full scope and user experience for each tool, were not as well understood, so establishing a fixed set of initial requirements was not feasible. An Agile Software Development (ASD) paradigm takes advantage of uncertainty, and plans for it, so it was deemed the most appropriate approach to create room for exploring novel concepts, and to mount a rapid and flexible response to inevitiably changing requirements. It facilitated the need for unprecedented collaboration between the Product Team (i.e. users from the Flight Control Team) and the Development Team (i.e. POIC systems engineers, developers, testers). This is a process shift in the development, test, and release of software from one that is prescriptive to one that is adaptive, which is necessary for these tools to have longevity. The application of ASD to the product development lifecycle permitted the timely incorporation of customer feedback, and, allowed for continuous quality improvements. This resulted in a suite of tools that are efficient, user-friendly, and enable POIF ground systems to support the increasing pace of payload science operations. ASD is not as much a set of prescriptive processes as it is a shift in mindset; one that moves from planning against change, to planning for change, thereby iteratively growing software towards user-defined value.

Albers, Cerese M.↗

Effects of exercise pressor reflex activation on carotid baroreflex function during exercise in humans

1. This investigation was designed to determine the contribution of the exercise pressor reflex to the resetting of the carotid baroreflex during exercise. 2. Ten subjects performed 3.5 min of static one-legged exercise (20 % maximal voluntary contraction) and 7 min dynamic cycling (20 % maximal oxygen uptake) under two conditions: control (no intervention) and with the application of medical anti-shock (MAS) trousers inflated to 100 mmHg (to activate the exercise pressor reflex). Carotid baroreflex function was determined at rest and during exercise using a rapid neck pressure/neck suction technique. 3. During exercise, the application of MAS trousers (MAS condition) increased mean arterial pressure (MAP), plasma noradrenaline concentration (dynamic exercise only) and perceived exertion (dynamic exercise only) when compared to control (P < 0.05). No effect of the MAS condition was evident at rest. The MAS condition had no effect on heart rate (HR), plasma lactate and adrenaline concentrations or oxygen uptake at rest and during exercise. The carotid baroreflex stimulus-response curve was reset upward on the response arm and rightward to a higher operating pressure by control exercise without alterations in gain. Activation of the exercise pressor reflex by MAS trousers further reset carotid baroreflex control of MAP, as indicated by the upward and rightward relocation of the curve. However, carotid baroreflex control of HR was only shifted rightward to higher operating pressures by MAS trousers. The sensitivity of the carotid baroreflex was unaltered by exercise pressor reflex activation. 4. These findings suggest that during dynamic and static exercise the exercise pressor reflex is capable of actively resetting carotid baroreflex control of mean arterial pressure; however, it would appear only to modulate carotid baroreflex control of heart rate.

Non-NASA Center↗

Delayed translational silencing of ceruloplasmin transcript in gamma interferon-activated U937 monocytic cells: role of the 3' untranslated region

Ceruloplasmin (Cp) is an acute-phase protein with ferroxidase, amine oxidase, and pro- and antioxidant activities. The primary site of Cp synthesis in human adults is the liver, but it is also synthesized by cells of monocytic origin. We have shown that gamma interferon (IFN-gamma) induces the synthesis of Cp mRNA and protein in monocytic cells. We now report that the induced synthesis of Cp is terminated by a mechanism involving transcript-specific translational repression. Cp protein synthesis in U937 cells ceased after 16 h even in the presence of abundant Cp mRNA. RNA isolated from cells treated with IFN-gamma for 24 h exhibited a high in vitro translation rate, suggesting that the transcript was not defective. Ribosomal association of Cp mRNA was examined by sucrose centrifugation. When Cp synthesis was high, i.e., after 8 h of IFN-gamma treatment, Cp mRNA was primarily associated with polyribosomes. However, after 24 h, when Cp synthesis was low, Cp mRNA was primarily in the nonpolyribosomal fraction. Cytosolic extracts from cells treated with IFN-gamma for 24 h, but not for 8 h, contained a factor which blocked in vitro Cp translation. Inhibitor expression was cell type specific and present in extracts of human cells of myeloid origin, but not in several nonmyeloid cells. The inhibitory factor bound to the 3' untranslated region (3'-UTR) of Cp mRNA, as shown by restoration of in vitro translation by synthetic 3'-UTR added as a "decoy" and detection of a binding complex by RNA gel shift analysis. Deletion mapping of the Cp 3'-UTR indicated an internal 100-nucleotide region of the Cp 3'-UTR that was required for complex formation as well as for silencing of translation. Although transcript-specific translational control is common during development and differentiation and global translational control occurs during responses to cytokines and stress, to our knowledge, this is the first report of translational silencing of a specific transcript following cytokine activation.

NASA Discipline Regulatory Physiology↗

Circadian oscillations of cytosolic and chloroplastic free calcium in plants

Tobacco and Arabidopsis plants, expressing a transgene for the calcium-sensitive luminescent protein apoaequorin, revealed circadian oscillations in free cytosolic calcium that can be phase-shifted by light-dark signals. When apoaequorin was targeted to the chloroplast, circadian chloroplast calcium rhythms were likewise observed after transfer of the seedlings to constant darkness. Circadian oscillations in free calcium concentrations can be expected to control many calcium-dependent enzymes and processes accounting for circadian outputs. Regulation of calcium flux is therefore fundamental to the organization of circadian systems.

NASA Discipline Plant Biology↗

Investigation of plasma instabilities in the Polar cusp

During the last six months, our efforts concentrated on studying the excitation of electromagnetic waves in the whistler frequency range by an anisotropic electron beam. A paper entitled 'Electron Cyclotron Wave Generation by Relativistic Electrons' was submitted to Journal of Geophysical Research and was accepted for publication. This paper is in collaboration with Dr. M. L. Goldstein at Goddard Space Flight Center. It was shown that an anisotropic electron beam (or gyrating electron beam) is capable of generating electron cyclotron waves with frequency from above to below the electron plasma frequency in a low density plasma. This instability may account for the observed Z mode and the electromagnetic component of auroral hiss in the Earth's polar region. The abstract of this paper is enclosed. For a high density plasma in which the electron plasma frequency is considerably higher than the electron cyclotron frequency, a new left-hand electromagnetic wave at whistler frequencies, which is also driven unstable by an anisotropic electron beam, was found. The basic notion of this new instability is the significant change of the dispersion equation due to the contribution of the beam component, which can shift the usual right hand whistler waves into left hand waves.

Wong, H. K.↗