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At least 829 records · Page 46

First direct comparison of high and low ionization line kinematics in active galactic nuclei

We present first results of a comparison of emission line shift properties for the high (HILs) and low (LILs) ionization lines in 43 low-reshift quasars. We identify a core sample of C IV lambda 1549 and hydrogen beta profiles with a wide distribution of red- and blueshifts (less than or equal to +/- 1000 km/sec). We also identify two tails in this distribution: one with large hydrogen beta redshifts (greater than or equal to 2000 km/sec) and another with large C IV blueshifts (greater than or equal to 1500 km/sec). The tails are mutually exclusive. All objects with extreme hydrogen beta redshift are radio loud, and all objects with extreme C IV blueshift are radio quiet. The core samples of smaller shifts can be most simply divided into: (1) hydrogen beta - a redshifted radio-loud population (related to the tail) and a radio-quiet population with mean shift near zero, and (2) C IV - a blueshifted radio-quiet population (related to the tail) and a radio-loud population with mean shift near zero. The results suggest fundamentally different kinematics for the HILs and LILs. They also suggest very different kinematics for radio-loud and radio-quiet active galactic nuclei. They also favor a predominance of radial motion in a large fraction of the sample.

Sulentic, J. W.↗

The occultation of SAO 78505 by Jupiter

On 13 December 1989, Jupiter occulted a star with visual magnitude 8.7 and an estimated K magnitude of 7. We observed the event from Kitt Peak, Arizona, using a 64 x 64 infrared camera at a wavelength of 2.16 microns. The resulting data on refractive defocusing of the stellar signal give information on the temperature of the jovian atmosphere at pressures approximately 2 to 10 microbar, at near-equatorial latitudes. These are the first new stellar-occulation data for the high jovian atmosphere since the widely observed occulation of beta Sco A and C in 1971. Because of improvements in instrumental capability, our data are comparable to the best beta Sco A data though the star is six magnitudes fainter. We derive a mean atmospheric temperature of 176 +/- 12 K on a level surface corresponding to an equatorial radius of 71,880 km and a pressure of 1.8 microbar at a jovi- centric latitude of 8 deg. This result complements the beta Sco results by providing improved precision at low jovicentric latitudes where the fainter star beta Sco C was used in 1971.

Hubbard, W. B.↗

Functional relationships among monitoring performance: Subjective report of thought process and compromising states of awareness

A biocybernetic system for use in adaptive automation was evaluated using EEG indices based on the beta, alpha, and theta bandwidths. Subjects performed a compensatory tracking task while their EEG was recorded and one of three engagement indices was derived: beta/(alpha + theta), beta/alpha, or 1/alpha. The task was switched between manual and automatic modes as a function of the subjects' level of engagement and whether they were under a positive or negative feedback condition. It was hypothesized that negative feedback would produce more switches between manual and automatic modes, and that the beta/(alpha + theta) index would produce the strongest effect. The results confirmed these hypotheses. There were no systematic changes in these effects over three 16-minute trials. Tracking performance was found to be better under negative feedback. An analysis of the different EEG bands under positive and negative feedback in manual and automatic modes found more beta power in the positive feedback/manual condition and less in the positive feedback/automatic condition. The opposite effect was observed for alpha and theta power. The implications of biocybernetic systems for adaptive automation are discussed.

Freeman, Frederick↗

Coronal magnetic field diagnostics via the Hanle effect of Lyman series lines

The magnetic field plays a major role in the physics of the solar corona. However, there are no direct measurements of this physical parameter. We describe a method that can provide the most direct determination of the vector magnetic field in the extended corona (i.e., at heliocentric heights between 1.2 R(solar radius) and 2.0 R(solar radius)). The method is based on polarimetric observations of UV lines of the Lyman series, that is, Lyman alpha (Ly-alpha), lambda 1216 A, Lyman beta (Ly-beta), lambda 1025 A, and Lyman gamma (Ly-gamma), lambda 972 A. These lines have a collisional and a resonantly scattered component. Linear polarization is induced in the resonant component by the anisotropy in the chromospheric radiation field that illuminates the corona. Magnetic fields can be suitably determined through the effects that they induce on this resonance polarization (Hanle effect). The Hanle effect of the Ly-alpha is sensitive to field strengths in the 10 - 100 gauss range. The resonance polarization of Ly-beta and Ly-gamma is sensitive, through the Hanle effect, to fields with strengths between 3 - 30 gauss, and 0.3 - 6 gauss, respectively. We describe a new method for separating the resonant from the collisional component of the Ly-beta and Ly-gamma; the method is based on the approximation, valid within 10%, that the collisional component of the Ly-alpha is negligible, in typical coronal conditions. From the intensity and the polarization of the resonant components of these Lyman lines, the strength and direction of coronal fields can be determined. We model the sensitivity of Hanle-effect diagnostics for different coronal structures (e.g., coronal holes and loops).

Fineschi, S.↗

Transition of slow shocks to fast shocks

As a CME pushes its way through preceding slower solar wind, large disturbances in the interaction region may evolve to form transient MHD shocks. The shocks can be slow shocks in the coronal space but they appear as fast shocks near 1 AU. We use a polytropic MHD model to study the transition of slow shocks to fast shocks in an interaction region sandwiched between a faster solar wind and a slower solar wind near the equatorial plane. The polytropic index controls the radial increase of Beta outside the interaction region. The transition process is attributed chiefly to the increase of Beta and theta to a lesser degree to the radial increase of the shock angle 0. Under the initial condition of small Beta and theta near 0.1 AU, the interaction region evolves to form a pair of slow shocks inside 0.15 AU. As the interaction region convects outward. the increases of B and theta cause a transition of the shock system between 0.15 and 0.3 AU from a slow shock pair to a double shock pair consisting of both slow and fast shocks. As the system moves outward, and Beta continue to increase, the fast shock grows stronger, and the slow shock becomes weaker. Eventually. the slow shocks fade away, and the shock system finally evolves to a pair of fast shocks. Parametric studies of the transition process are carried out for shocks formed over a wide range of disturbances.

Whang, Y. C.↗

Validation of Spectral Analysis as a Noninvasive Tool to Assess Autonomic Regulation of Cardiovascular Function

A major focus of our program has been to develop a sensitive noninvasive procedure to quantify early weightlessness-induced changes in cardiovascular function or potential dysfunction. Forty studies of healthy young volunteers (10 men and 10 women, each studied twice) were conducted to determine changes in the sympatho-vagal balance of autonomic control of cardiovascular regulation during graded headward and footward blood volume shifts. Changes in sympatho-vagal balance were classified by changes in the mean levels and spectral content of cardiovascular variables and verified by changes in circulating levels of catecholamines and pancreatic polypeptide. Possible shifts in intra/extravascular fluid were assessed from changes in hematocrit and plasma mass density while changes in the stimulus to regulate plasma volume were determined from Plasma Renin Activity (PRA). Autonomic blockade was used to unmask the relative contribution of sympathetic and parasympathetic efferent influences in response to 10 min each of 0, 20 and 40 mmHg Lower Body Negative Pressure (LBNP) and 15 and 30 mmHg Positive Pressure (LBPP). The combination of muscarinic blockade with graded LBNP and LBPP was used to evoke graded increases and decreases in sympathetic activity without parasympathetic contributions. The combination of beta blockade with graded LBNP and LBPP was used to produce graded increases and decreases in parasympathetic activity without beta sympathetic contributions. Finally, a combination of both beta and muscarinic blockades with LBNP and LBPP was used to determine the contribution from other, primarily alpha adrenergic, sources. Mean values, spectral analyses and time frequency analysis of R-R interval (HR), Arterial Pressure (AP), peripheral blood flow (RF), Stroke Volume (SV) and peripheral resistance (TPR) were performed for all phases of the study. Skin blood Flow (SF) was also measured in other studies and similarly analyzed. Spectra were examined for changes in three frequency regions (low 0.006 - 0.005 Hz (LF), mid 0.05 - 0.15 Hz (W), and high 0.15 - 0.45 Hz (EF)). The primary objective of the study was to indicate which changes in the mean values and/or spectra of cardiovascular variables consistently correlated with changes in sympatho-vagal balance in response to headward and footward fluid shifts. A secondaey objective was to quantify the vascular and extravascular fluid shifts evoked by LBNP and LBPP. The principal hypothesis being tested was that headward fluid shifts would evoke an increase in parasympathetic activity and footward fluid shifts would evoke an increase in sympathetic activity both of which would be detected by spectral analysis and verified by circulating hormones. Hematocrit (HCT), plasma mass density and plasma renin activity increased with muscarinic blockade and with LBNP, a response indicative of a plasma shift to extravascular spaces. Beta blockade alone or after muscarinic blockade had no effect on HCT or plasma mass density. With respect to intravascular fluid volume distribution, LBNP and LBPP produced sufficient upper body vascular fluid shifts to evoke appropriate autonomic regulatory responses.

Knapp, Charles F.↗

Submillimeter Imaging of Dust Around Main Sequence Stars

This grant was to image circumstellar dust disks surrounding main-sequence stars. The delivery of the SCUBA detector we had planned to use for this work was delayed repeatedly, leading us to undertake a majority of the observations with the UKT14 submillimeter detector at the JCMT (James Clerk Maxwell Telescope) and optical imagers and a coronagraph at the University of Hawaii 2.2-m telescope. Major findings under this grant include: (1) We discovered 5 asymmetries in the beta Pictoris regenerated dust disk. The discovery of these asymmetries was a surprise, since smearing due to Keplerian shear should eliminate most such features on timescales of a few thousand years. One exception is the "wing tilt" asymmetry, which we interpret as due to the scattering phase function of dust disk particles. From the wing tilt and a model of the phase function, we find a disk plane inclination to the line of sight of < 5 degrees. Other asymmetries (e.g. the butterfly asymmetry) suggest a disk that has been recently disturbed. We searched for possible nearby perturbers but found no clear candidates. Low mass stars (M dwarfs) and brown dwarfs would have fallen beneath the sensitivity threshhold of our survey, however. (2) We calculated a set of disk models to assess the detectability of dust disks around stars as a function of (a) distance, (b) disk, inclination (c) dust optical depth/mass, and (d) imaging resolution. These models guided our observational strategy on Mauna Kea. (3) We performed a coronagraphic survey of approx. 100 main-sequence stars in search of additional examples of circumstellar disks. The best new candidate disk, around the 5 M(sun) star BD+31deg.643, is distinguished by its large extent (few x 10( exp 3) AU). This disk, if real, cannot be rotationally supported. We suggest that the dust particles are ejected from a smaller, unseen disk (Kuiper Belt?) by strong radiation pressure forces due to the high luminosity central star. (4) SCUBA images of circumstellar dust disks were obtained at 850 gm in 1997/8. These images show extended, asymmetric emission, but have a signal-to-noise ratio too low to permit disk mapping to large projected distances. Our images of beta Pic, in particular, are in agreement with those obtained by Holland et al., and appear to confirm the blob-structure reported first by these authors. We have not yet been able to confirm that the structure is intrinsic to the disk, since beta Pic is at -50 degree declination, and suitable observing opportunities from northern latitudes are comparatively rare (even at the +20 degree latitude of JCMT). It is possible, for instance, that the main 850 micro-m blob is merely a galaxy or other high-z source projected onto the beta Pic mid-plane.

Jewitt, David↗

Gravity and Skeletal Growth

Two simultaneous experiments were performed using 5-week-old male Sprague Dawley rats; in one study, the rats were flown in low earth orbit; in the other study, the hindlimbs of the growing rats were elevated to prevent weight bearing. Following 9 d of unloading, weight bearing was restored for 4, 28, and 76 hrs. Afterwards, additional hindlimb unloading experiments were performed to evaluate the skeletal response to 0, 2, 4, 6, 8, 10, 12, 16, and 24 hrs of restored weight bearing following 7 d of unloading. Cancellous and cortical bone histomorphometry were evaluated in the left tibia at the proximal metaphysis and in the left femur at mid-diaphysis, respectively. Steady-state mRNA levels for bone matrix proteins and skeletal signaling peptides were determined in total cellular RNA extracted from trabeculae from the right proximal tibiametaphysis and periosteum from the right femur. Spaceflight and hindlimb unloading each resulted in cancellous osteopenia, as well as a tendency towards decreased periosteal bone formation. Both models for skeletal unloading resulted in site specific reductions in mRNA levels for transforming growth factor-beta (sub 1) (TGF-beta) osteocalcin (OC), and prepro-alpha (I) subunit of type 1 collagen (collagen) and little or no changes in mRNA levels for glyceraldehyde-3-phosphate dehydrogenase (GAP) and insulin-like growth factor I (IGF-I). Restoration of normal weight bearing resulted in transient increases in mRNA levels for the bone matrix proteins and TGF-beta in the proximal metaphysis and periosteum and no changes in either GAP or IGF-I mRNA levels. The timecourse for the response differed between the two skeletal compartments; the tibial metaphysis responded much more quickly to reloading. These results suggest that the skeletal adaptation to acute physiological changes in mechanical usage are mediated, in part, by changes in mRNA levels for bone matrix proteins and TGF-beta.

Morey-Holton, Emily↗

Non-Destructive Evaluation Method and Apparatus for Measuring Acoustic Material Nonlinearity

An acoustic non-linearity parameter (beta) measurement method and system for Non-Destructive Evaluation (NDE) of materials and structural members obviates the need for electronic calibration of the measuring equipment. Unlike known substitutional measuring techniques requiring elaborate calibration procedures, the electrical outputs of the capacitive detector of a sample with known beta and the test sample of unknown beta are compared to determine the unknown beta. In order to provide the necessary stability of the present-inventive reference-based approach, the bandpass filters of the measurement system are maintained in a temperature-controlled environment, and the line voltage supplied to said amplifiers is well-regulated.

William T Yost↗

Pitched and Yawed Circular Jets in Cross-Flow

Results from an experimental investigation of flow field generated by pitched and yawed jets discharging from a flat plate into a cross-flow are presented. The circular jet was pitched at alpha = 20 degrees and 45 degrees and yawed between beta = 0 degrees and 90 degrees in increments of 15 degrees. The measurements were performed with two X-wires providing all three components of velocity and turbulence intensity. These data were obtained at downstream locations of x = 3, 5, 10 and 20, where the distance x normalized by the jet diameter, is measured from the center of the orifice. Data for all configurations were acquired at a momentum-flux ratio J = 8. Additionally, for selected angles and locations, surveys were conducted for J = 1.5, 4, and 20. As expected, the jet penetration is found to be higher at larger alpha. With increasing beta the jet spreads more. The rate of reduction of peak streamwise vorticity, with the downstream distance is significantly lessened at higher alpha but is found to be practically independent of alpha. Thus, at the farthest measurement station x = 20, omega(sub xmax) is about five times larger for beta = 0 degrees compared to the levels at beta = 0 degrees. Streamwise velocity within the jet-vortex structure is found to depend on the parameter J. At J = 1.5 and 4, 'wake-like' velocity profiles are observed. In comparison, a 'jet-like' overshoot is present at higher J.

Milanovic, Ivana M.↗

Assessing Impacts of Global Warming on Tropical Cyclone Tracks

A new approach is proposed to assess the possible impacts of the global climate change on tropical cyclone (TC) tracks in the western North Pacific (WNP) basin. The idea is based on the premise that the future change of TC track characteristics is primarily determined by changes in large-scale environmental steering flows. It is demonstrated that the main characteristics of the current climatology of TC tracks can be derived from the climatological mean velocity field of TC motion by using a trajectory model. The climatological mean velocity of TC motion, which is composed of the large-scale steering and beta drift, is determined on each grid of the basin. The mean beta drift is estimated from the best track data, and the mean large-scale steering flow is computed from the NCEP/NCAR reanalysis for the current climate state. The derived mean beta drift agrees well with the results of previous observational and numerical studies in terms of its direction and magnitude. The outputs of experiments A2 and B2 of the Geophysical Fluid Dynamics Laboratory (GFDL) R30 climate model suggest that the subtropical high will be persistently weak over the western part of the WNP or shift eastward during July-September in response to the future climate change. By assuming that the mean beta drift in the future climate state is unchanged, the change in the general circulation by 2059 will decrease the TC activities in the WNP, but favor a northward shift of typical TC tracks. As a result, the storm activities in the South China Sea will decrease by about 12%, while the Japan region will experience an increase of TCs by 12-15%. During the period of 2000-2029, the tropical storms that affect the China region will increase by 5-6%, but return to the current level during 2030-2059. It is also suggested that, during the period of 2030-2059 tropical storms will more frequently affect Japan and the middle latitude region of China given that the formation locations remain the same as in the current climate state.

Wu, Li-Guang↗

Kinetics of Organic Transformations Under Mild Aqueous Conditions: Implications for the Origin of Life and Its Metabolism

The rates of thermal transformation of organic molecules containing carbon, hydrogen, and oxygen were systematically examined in order to identify the kinetic constraints that governed origin-of-life organic chemistry under mild aqueous conditions. Arrhenius plots of the kinetic data were used to estimate the reaction half-life at 50 C, and to reveal the effect of functional groups on reactivity. This survey showed that hydrocarbons and organic substances containing a single oxygenated group were kinetically the most stable (i. e. acetate decarboxylation half-life was l0(exp 18) years at 50 C); whereas, organic substances containing two oxygenated groups in which one group was a beta-positioned carbonyl group were the most reactive (i. e. acetoacetate decarboxylation half-life was l0(exp-2) years at 50 C). Of all functional groups the beta-positioned carbonyl group (aldehyde or ketone) was the strongest activating group, giving rates of reaction that were up to 10(exp 24)-times faster than rates of similar molecules lacking the beta-carbonyl group. From this knowledge of organic reactivity and the inherent constraints of autocatalytic processes, we concluded that an origins-of-life process based on autocatalytic transformation of C,H,O-substrates was constrained to using the most reactive organic molecules that contain alpha- or beta-carbonyl groups, since small autocatalytic domains of plausible catalytic power that used less reactive substrates could not carry out chemical transformations fast enough to prevent catastrophic efflux (escape) of reaction intermediates. Knowledge of the kinetics of organic transformations is useful, not only in constraining the chemistry of the earliest autocatalytic process related to the origin of life, but also in establishing the relative reactivity of organic molecules on the early Earth and other planets that may or may not be related to the origin of life.

Weber, Arthur L.↗

Steps Toward Determination of the Size and Structure of the Broad-Line Region in Active Galactic Nuclei XVI: A 13 Year Study of Spectral Variability in NGC 5548

We present the final installment of an intensive 13 year study of variations of the optical continuum and broad H beta emission line in the Seyfert 1 galaxy NGC 5548. The database consists of 1530 optical continuum measurements and 1248 H beta measurements. The H beta variations follow the continuum variations closely, with a typical time delay of about 20 days. However, a year-by-year analysis shows that the magnitude of emission-line time delay is correlated with the mean continuum flux. We argue that the data are consistent with the simple model prediction between the size of the broad-line region and the ionizing luminosity, r is proportional to L(sup 1/2)(sub ion). Moreover, the apparently linear nature of the correlation between the H beta response time and the nonstellar optical continuum F(sub opt) arises as a consequence of the changing shape of the continuum as it varies, specifically F(sub opt) is proportional to F(sup 0.56)(sub UV).

Peterson, B. M.↗

Modulation of lens cell adhesion molecules by particle beams

Cell adhesion molecules (CAMs) are proteins which anchor cells to each other and to the extracellular matrix (ECM), but whose functions also include signal transduction, differentiation, and apoptosis. We are testing a hypothesis that particle radiations modulate CAM expression and this contributes to radiation-induced lens opacification. We observed dose-dependent changes in the expression of beta 1-integrin and ICAM-1 in exponentially-growing and confluent cells of a differentiating human lens epithelial cell model after exposure to particle beams. Human lens epithelial (HLE) cells, less than 10 passages after their initial culture from fetal tissue, were grown on bovine corneal endothelial cell-derived ECM in medium containing 15% fetal bovine serum and supplemented with 5 ng/ml basic fibroblast growth factor (FGF-2). Multiple cell populations at three different stages of differentiation were prepared for experiment: cells in exponential growth, and cells at 5 and 10 days post-confluence. The differentiation status of cells was characterized morphologically by digital image analysis, and biochemically by Western blotting using lens epithelial and fiber cell-specific markers. Cultures were irradiated with single doses (4, 8 or 12 Gy) of 55 MeV protons and, along with unirradiated control samples, were fixed using -20 degrees C methanol at 6 hours after exposure. Replicate experiments and similar experiments with helium ions are in progress. The intracellular localization of beta 1-integrin and ICAM-1 was detected by immunofluorescence using monoclonal antibodies specific for each CAM. Cells known to express each CAM were also processed as positive controls. Both exponentially-growing and confluent, differentiating cells demonstrated a dramatic proton-dose-dependent modulation (upregulation for exponential cells, downregulation for confluent cells) and a change in the intracellular distribution of the beta 1-integrin, compared to unirradiated controls. In contrast, there was a dose-dependent increase in ICAM-1 immunofluorescence in confluent, but not exponentially-growing cells. These results suggest that proton irradiation downregulates beta 1-integrin and upregulates ICAM-1, potentially contributing to cell death or to aberrant differentiation via modulation of anchorage and/or signal transduction functions. Quantification of the expression levels of the CAMs by Western analysis is in progress.

NASA Discipline Radiation Health↗

Antipeptide antibodies that can distinguish specific subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.)

The amino acid sequences of the beta and gamma subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.) root nodules are very similar. However, there are small regions within the sequences that are significantly different between the two polypeptides. The sequences between amino acids 2 and 9 and between 264 and 274 are examples. Three peptides (gamma 2-9, gamma 264-274, and beta 264-274) corresponding to these sequences were synthesized. Antibodies against these peptides were raised in rabbits and purified with corresponding peptide-Sepharose affinity chromatography. Western blot analysis of polyacrylamide gel electrophoresis of bean nodule proteins demonstrated that the anti-beta 264-274 antibodies reacted specifically with the beta polypeptide and the anti-gamma 264-274 and anti-gamma 2-9 antibodies reacted specifically with the gamma polypeptide of the native and denatured glutamine synthetase. These results showed the feasibility of using synthetic peptides in developing antibodies that are capable of distinguishing proteins with similar primary structures.

NASA Program NSCORT↗

Temperature modulates the cell wall mechanical properties of rice coleoptiles by altering the molecular mass of hemicellulosic polysaccharides

The present study was conducted to investigate the mechanism inducing the difference in the cell wall extensibility of rice (Oryza sativa L. cv. Koshihikari) coleoptiles grown under various temperature (10-50 degrees C) conditions. The growth rate and the cell wall extensibility of rice coleoptiles exhibited the maximum value at 30-40 degrees C, and became smaller as the growth temperature rose or dropped from this temperature range. The amounts of cell wall polysaccharides per unit length of coleoptile increased in coleoptiles grown at 40 degrees C, but not at other temperature conditions. On the other hand, the molecular size of hemicellulosic polysaccharides was small at temperatures where the cell wall extensibility was high (30-40 degrees C). The autolytic activities of cell walls obtained from coleoptiles grown at 30 and 40 degrees C were substantially higher than those grown at 10, 20 and 50 degrees C. Furthermore, the activities of (1-->3),(1-->4)-beta-glucanases extracted from coleoptile cell walls showed a similar tendency. When oat (1-->3),(1-->4)-beta-glucans with high molecular mass were incubated with the cell wall enzyme preparations from coleoptiles grown at various temperature conditions, the extensive molecular mass downshifts were brought about only by the cell wall enzymes obtained from coleoptiles grown at 30-40 degrees C. There were close correlations between the cell wall extensibility and the molecular mass of hemicellulosic polysaccharides or the activity of beta -glucanases. These results suggest that the environmental temperature regulates the cell wall extensibility of rice coleoptiles by modifying mainly the molecular mass of hemicellulosic polysaccharides. Modulation of the activity of beta-glucanases under various temperature conditions may be involved in the alteration of the molecular size of hemicellulosic polysaccharides.

Cellulose/chemistry/metabolism↗

Direct binding of F actin to the cytoplasmic domain of the alpha 2 integrin chain in vitro

The transmembrane integrins have been shown to interact with the cytoskeleton via noncovalent binding between cytoplasmic domains (CDs) of integrin beta chains and various actin binding proteins within the focal adhesion complex. Direct or indirect integrin alpha chain CD binding to the actin cytoskeleton has not been reported. We show here that actin, as an abundant constituent of focal adhesion complex proteins isolated from fibroblasts, binds strongly and specifically to alpha 2 CD, but not to alpha 1 CD peptide. Similar specific binding to alpha 2 CD peptide was seen for highly purified F actin, free of putative actin-binding proteins. The bound complex of actin and peptide was visualized directly by coprecipitation, and actin binding was abrogated by removal of a five amino acid sequence from the alpha 2 CD peptide. Our findings may explain the earlier observation that, while integrins alpha 2 beta 1 and alpha 1 beta 1 both bind to collagen, only alpha 2 beta 1 can mediate contraction of extracellular collagen matrices.

Non-NASA Center↗

The glycan keratan sulfate in inner ear crystals

The otoconial matrix (OM) of chicks (Gallus domesticus) inner ear was analyzed. Histochemically the OM was reacted with phosphotungstic acid (PTA) and immunohistochemically with the monoclonal antibody antikeratan sulfate (antiKS). The OM was digested with the enzyme endo-beta-galactosidase (E beta Galase) or separated by 1D and 2D gel electrophoresis. PTA which reacts with glycoproteins precipitated the OM, suggesting that the OM contains glycoproteins. A central core in each crystal had no PTA staining, suggesting that the core lacked glycoproteins. Anti KS antibody stained the OM with increased density in older embryos as determined by color thresholding. E beta Galase, which cleaves the lactosamine repeating units in KS, decreased the immunostain by 30% in the OM and by 20% in the cartilage. The OM from the utricle, saccule and macula lagena contained similar molecular weight bands. Five dense bands in the OM were less dense in tissue and blood controls, suggesting that such bands are enriched in the OM. Isoelectric focusing of the OM showed a negatively charged high molecular weight smear not present in blood and faint in tissue controls. The high affinity of the OM for the cationic PTA stain, the strong immunohistochemical reaction of the OM with anti KS antibody and high molecular weight negative smear in 2D gels taken together suggest that: a) the OM contains large amounts of glycoproteins and glycans, one of which is keratan sulfate, because its immuno stain with antiKS antibody was decreased by the enzyme E beta Galase, b) the utricle, saccule and macula lagena may have similar composition, and c) the concentration of KS may increase gradually until complete mineralization of the OM is reached.

Non-NASA Center↗