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Gravity-Off-loading System for Large-Displacement Ground Testing of Spacecraft Mechanisms

Gravity-off-loading of deployable spacecraft mechanisms during ground testing is a long-standing problem. Deployable structures which are usually too weak to support their own weight under gravity require a means of gravity-off-loading as they unfurl. Conventional solutions to this problem have been helium-filled balloons or mechanical pulley/counterweight systems. These approaches, however, suffer from the deleterious effects of added inertia or friction forces. The changing form factor of the deployable structure itself and the need to track the trajectory of the center of gravity also pose a challenge to these conventional technologies. This paper presents a novel testing apparatus for high-fidelity zero-gravity simulation for special application to deployable space structures such as solar arrays, magnetometer booms, and robotic arms in class 100,000 clean room environments

Han, Olyvia↗

Z-Pinch Fusion Propulsion

Fusion-based nuclear propulsion has the potential to enable fast interplanetary transportation. Shorter trips are better for humans in the harmful radiation environment of deep space. Nuclear propulsion and power plants can enable high Ispand payload mass fractions because they require less fuel mass. Fusion energy research has characterized the Z-Pinch dense plasma focus method. (1) Lightning is form of pinched plasma electrical discharge phenomena. (2) Wire array Z-Pinch experiments are commonly studied and nuclear power plant configurations have been proposed. (3) Used in the field of Nuclear Weapons Effects (NWE) testing in the defense industry, nuclear weapon x-rays are simulated through Z-Pinch phenomena.

Miernik, Janie↗

Surface Systems and Interface Standardization

A key contribution to surface systems sustainability on Lunar and planetary surfaces is commonality between hardware and software interfaces. Generic interfaces for data, power, and fluids will reduce risk, promote interoperability, and define standard interfaces across surface exploration projects and programs. Standardized interfaces would be advantageous for improving efficiency and reducing overall complexity, which are critical considerations for future space exploration. Furthermore, it will provide cost reductions to NASA’s Artemis program over its life cycle (in Operations & Maintenance (O&M) and Logistics). Unique proprietary interfaces if considered or allowed would not only increase complexity but also add cost to the programs. Of course, understanding what will work and not work effectively in these unique environments such as the lunar surface is important. These unique environments require much needed intelligent design, prototyping, comprehensive testing, and field experience, utilizing consensus on common interface solutions.

Lunar↗

Differential Gene Expression in A Cross-Feeding Two-Species Model Microbial Community Under Simulated Microgravity and Deep-Space Radiation

A long-term goal of space biology is to understand interspecies microbial interactions in space. Presently, little is known about the combined effect of microgravity and ionizing radiation on bacterial community response when species are interdependent through exchange of metabolites in fluid medium (cross-feeding). Microgravity is expected to slow interspecies mass transfer and growth in cross-feeding communities in the low-shear, diffusion-limited environment, while ionizing radiation may influence stress response to direct (DNA damage) and indirect damage (ROS). Using a well-understood, two-species (Escherichia coli and Salmonella enterica) microbial community engineered to be a model for studying cross-feeding, we simulated galactic cosmic rays (GCRsim) and microgravity to test the hypothesis: exposure to ionizing radiation causes cell damage or stress, altering transcriptomic community responses in metabolically interdependent cells, which is exacerbated by microgravity. We expect to see differential gene expression between cross-feeding and non-cross-feeding communities. We measured GCRsim effects on growth and gene expression in well-mixed versus simulated-microgravity conditions and in cross-feeding and non-cross-feeding medium. Microbial cultures were inoculated into liquid medium in rotating wall vessels (RWV) with different rotation rates: 5 RPM (simulated microgravity) and 50 RPM (well-mixed). The E. coli-S. enterica consortium, under simulated microgravity, were exposed to 500 mGy of Simplified 5-ion Galactic Cosmic Ray Simulation for 2 hours at Brookhaven National Lab. We harvested samples 40 minutes after irradiation for extraction and sequencing (NASA GeneLab). Here we present the differential gene expression analysis results, which reveal altered transcriptomic community responses, even where growth rate differences are not observed. Gene expression of these actively metabolizing microbial communities in GCRsim may illuminate molecular mechanisms of microbial interactions in space. Understanding how microbial community gene expression, metabolism, and other cellular processes are influenced by spaceflight stressors can inform the use of microbes in human life support for low Earth orbit missions and beyond.

microgravity↗

State-of-the-Art: Small Spacecraft Technology

When the first edition of NASA’s Small Spacecraft Technology State-of-the-art report was published in 2013, 247 CubeSats and 105 other non-CubeSat small spacecraft under 50 kilograms (kg) had been launched worldwide, representing less than 2% of launched mass into orbit over multiple years. In 2013 alone, around 60% of the total spacecraft launched had a mass under 600 kg, and of those under 600 kg, 83% were under 200 kg and 37% were nanosatellites (1). Of the total 1,849 spacecraft launched in 2021, 94% were small spacecraft with an overall mass under 600 kg, and of those under 600 kg, 40% were under 200 kg, and 11% were nanosatellites (1). Since 2013, the fight heritage for small spacecraft has increased by over 30% and has become the primary source to space access for commercial, government, private, and academic institutions. The total number of spacecraft launched in the past 10 years is 5,681 and 45% of those had a mass. As with all previous editions of this report, the 2022 edition captures and distills a wealth of new information available on small spacecraft systems from NASA and other publicly available sources. This report is limited to publicly available information and cannot reflect major advances in development that are not publicly disclosed. We encourage any opportunity to publish mission outcomes and technology development milestones (e.g., via conference papers, press releases, company website) so they can be reflected in this report. Overall, this report is a survey of small spacecraft technologies sourced from open literature; it does not endeavor to be an original source, and only considers literature in the public domain to identify and classify devices. Commonly used sources for data include manufacturer datasheets, press releases, conference papers, journal papers, public filings with government agencies, news articles, presentations, the compendium of databases accessed via NASA’s Small Spacecraft Systems Virtual Institute (S3VI) Information Search, and engagement with companies. Data not appropriate for public dissemination, such as proprietary, export controlled, or otherwise restricted data, are not considered. As a result, this report includes many dedicated hours of desk research performed by subject matter experts reviewing resources noted above. Content in this 2022 edition is based on data available by October 2022. This report should not be considered as a comprehensive overview of all the technologies but a great reference for the current state-of-the-art SmallSat technologies. The organizational approach for each chapter is relatively consistent with previous editions and includes an introduction of the technology, current development status of the technology’s procurable systems, and summary tables of technologies surveyed. The content in each chapter is uniquely organized to present a mini-stand-alone report on spacecraft subsystems. As in previous years, chapters include information from previous editions but are updated with new and maturating technologies and reference missions. Tables in each section provide a convenient summary of the technologies discussed, with explanations and references in the body text. The authors have attempted to isolate trends in the small spacecraft industry to point out which technologies have been adopted after successful demonstration missions. Lastly, the authors tried to use the terms “SmallSat,” “microsatellite,” “nanosatellite,” and “CubeSat” in a consistent manner, even as these terms are often used interchangeably in the space industry. Every subsystem chapter contains updated information to reflect the growth in the small spacecraft market. Significant changes are included in several chapters. The “Complete Spacecraft Platforms” chapter now includes information on the two main market options, hosted payload services and dedicated buses. The “Power” chapter provides information on the development of solid-state batteries with significantly higher energy than the current state-of-theart lithium-ion batteries. A large effort was made to update the “Communications” chapter to appropriately capture the recent technology maturation of optical communications for SmallSats. The “Ground Data Systems and Mission Operations” chapter was updated to reflect the recent establishment of the Near Space Network and influx of SmallSat Optical Ground Stations. The “Guidance, Navigation and Control” chapter was updated to include Lidar sensor technology. The “Deorbit Systems” chapter includes a discussion of recently proposed changes by the Federal Communications Commission (FCC) to limit a spacecraft’s lifetime to no longer than 5 years after end-of-mission. The “Identification and Tracking” Chapter includes updated information on the progress of SmallSat tracking. Finally, this report now encompasses technology funded by NASA’s Small Spacecraft Technology (SST) program’s SmallSat Technology Partnerships (STP) initiative which is described further in this Introduction. The reader can find the included SST technology in the “On the Horizon” section of the “Thermal Systems”, “Communications”, and “Guidance, Navigation, and Control” chapters. A central element of this report is to list state-of-the-art technologies by NASA standard Technology Readiness Level (TRL) as defined by the 2020 NASA Engineering Handbook, found in NASA NPR 7123.1C NASA Systems Engineering Processes and Requirements. The authors have endeavored to independently verify the TRL value of each technology by reviewing and citing published test results or publicly available data to the best of their ability. Where test results and data disagree with vendors’ own advertised TRL, the authors have attempted to engage the vendors to discuss the discrepancy. Readers are strongly encouraged to follow the references cited in the literature describing the full performance range and capabilities of each technology. Readers of this report should reach out to individual companies to further clarify information. It is important to note that this report takes a broad system-level view. To attain a high TRL, the subsystem must be in a flight-ready configuration with all supporting infrastructure—such as mounting points, power conversion, and control algorithms—in an integrated unit. An accurate TRL assessment requires a high degree of technical knowledge on a subject device, and an in-depth understanding of the mission (including interfaces and environment) on which the device was flown. There is variability in TRL values depending on design factors for a specific technology. For example, differences in TRL assessment based on the operating environment may result from the thermal environment, mechanical loads, mission duration, or radiation exposure. If a technology has flown on a mission without success, or without providing valid confirmation to the operator, such claimed “flight heritage” was discounted. The authors believe TRLs are most accurately determined when assessed within the context of a program’s unique requirements. While the overall capability of small spacecraft has matured since the 2021 edition of this report, technologies are still being developed to make deep space SmallSat missions more routine and more cost effective. Future editions of this report may include content dedicated to the rapidly growing fields of assembly, integration, and testing services, and mission modeling and simulation–all of which are now extensively represented at small spacecraft conferences. Many of these subsystems and services are still in their infancy, but as they evolve and reliable conventions and standards emerge, the next iteration of this report may also evolve to include additional chapters.

Bruce Yost↗

A study of hydrogen environment effects on microstructure property behavior of NASA-23 alloy and related alloy systems

This work is part of the overall advanced main combustion chamber (AMCC) casting characterization program of the Materials and Processes Laboratory of the Marshall Space Flight Center. The influence of hydrogen on the tensile properties and ductility behavior of NASA-23 alloy were analyzed. NASA-23 and other referenced alloys in cast and hipped conditions were solution treated and aged under selected conditions and characterized using optical metallography, scanning electron microscopy, and electron microprobe analysis techniques. The yield strength of NASA-23 is not affected much by hydrogen under tensile tests carried at 5000 psig conditions; however, the ultimate strength and ductility properties are degraded. This implies that the physical mechanisms operating would be related to the plastic deformation process. The fracture surfaces characteristics of NASA-23 specimens tensile tested in hydrogen, helium, and air were also analyzed. These revealed surface cracks around specimen periphery with the fracture surface showing a combination of intergranular and transgranular modes of fracture. It is seen that the specimens charged in hydrogen seem to favor a more brittle fracture mode in comparison to air and helium charged specimens. The AMCC casting characterization program is to be analyzed for their hydrogen behavior. As a result of this program, the basic microstructural factors and fracture characteristics in some cases were analyzed.

Diwan, Ravinder M.↗

Results from testing and analysis of solar cells flown on LDEF

A brief discussion of the solar cell experiments flown on the Long Duration Exposure Facility (LDEF) is provided. The information presented is a collation of results published by the various experimenters. This process of collation and documentation is an ongoing Systems Special Investigation Group (SIG) effort. There are four LEO environments, operating individually and/or synergistically, that cause performance loss in solar cells: meteoroid and space debris, atomic oxygen, ultraviolet radiation, and charged particle radiation. In addition, the effects of contamination caused by outgassing of materials used on the specific spacecraft play a role in decreasing the light being transmitted through the coverglass and adhesive to the solar cell. From the results presented on the solar cells aboard LDEF, the most extensive degradation of the solar cells came from impacts and the resulting cratering. The extent of the damage to the solar cells was largely dependent upon the size and energy of the meteoroids or space debris. The other cause of degradation was reduced light reaching the solar cell. This was caused by contamination, UV degradation of coverglass adhesive, and/or atomic oxygen/UV degradation of antireflection coatings.

Dursch, Harry↗

Protoflight photovoltaic power module system-level tests in the space power facility

Work Package Four, which includes the NASA-Lewis and Rocketdyne, has selected an approach for the Space Station Freedom Photovoltaic (PV) Power Module flight certification that combines system level qualification and acceptance testing in the thermal vacuum environment: The protoflight vehicle approach. This approach maximizes ground test verification to assure system level performance and to minimize risk of on-orbit failures. The preliminary plans for system level thermal vacuum environmental testing of the protoflight PV Power Module in the NASA-Lewis Space Power Facility (SPF), are addressed. Details of the facility modifications to refurbish SPF, after 13 years of downtime, are briefly discussed. The results of an evaluation of the effectiveness of system level environmental testing in screening out incipient part and workmanship defects and unique failure modes are discussed. Preliminary test objectives, test hardware configurations, test support equipment, and operations are presented.

Rivera, Juan C.↗

Protoflight photovoltaic power module system-level tests in the Space Power Facility

Work Package Four, which includes the NASA-Lewis and Rocketdyne, has selected an approach for the Space Station Freedom Photovoltaic (PV) Power Module flight certification that combines system level qualification and acceptance testing in the thermal vacuum environment: the 'protoflight' vehicle approach. This approach maximizes ground test verification to assure system level performance and to minimize risk of on-orbit failures. The preliminary plans for system level thermal vacuum environmental testing of the protoflight PV Power Module in the NASA-Lewis Space Power Facility (SPF) are addressed. Details of the facility modifications to refurbish SPF, after 13 years of downtime, are briefly discussed. The results of an evaluation of the effectiveness of system level environmental testing in screening out incipient part and workmanship defects and unique failure modes are discussed. Preliminary test objectives, test hardware configurations, test support equipment, and operations, are presented.

Rivera, Juan C.↗

Determination of the mass properties of a manipulator

Space applications for telerobotics requires a manipulator that is robust to the large payload variations that occur in a zero-g environment. One approach to provide the required performance for this large payload variation is to provide inertial decoupling to the controller. The equations to generate the required joint torques can be obtained in several manners but the independent parameters required to perform the calculations are difficult to obtain. This presentation consists of an overview of the inertial decoupling control system and discusses how to reduce the inertial parameter set to a minimum and obtain the required parameter set to a minimum and obtain the required parameter vector using sensed joint positions and torques. In addition, as telerobotic technology becomes more prevalent in the space environments the ability to emulate the effect of zero-g in a one-g environment is critical in learning how to perform tasks in space. To emulate the zero-g environment it is necessary to remove the effect of gravity on the manipulator by determining the feedforward joint torques. A subset of the algorithm developed to obtain the decoupling parameters is used to obtain the gravity offload parameters. These parameters are used to eliminate the effect of gravity. The algorithm is validated on Martin Marietta/NASA Langley's, 7 Degrees-Of-Freedom (DOF) Flight Telerobotic Servicer Hydraulic Manipulator Test Bed. A video portraying actual results of the algorithm is provided.

Goldenberg, Stewart↗

The Use of Environmental Test Facilities for Purposes Beyond Their Original Design

Increasing demands from space flight project offices are requiring environmental testing facilities to become more versatile with increased capabilities. At the same time, maintaining a cost-effective approach to test operations has driven efforts to use these facilities for purposes beyond their original design. This paper presents an overview of the Jet Propulsion Laboratory's efforts to provide JPL's space flight projects with test facilities to meet unique test requirements and to serve the needs of selected outside customers. The large number of recent Mars Missions, including the Mars Pathfinder project, have required testing of components and systems in a Martian surface environment in facilities originally designed for deep space testing. The unique problems associated with performing these tests are discussed, along with practical solutions. Other unique test requirements are discussed including the use of space simulation chambers for testing high altitude balloon gondolas and the use of vacuum chambers for system level test firing of an ion propulsion engine.

Fisher, Terry C.↗

Effect of Pressure on Piloted Ignition Delay of PMMA

In order to reduce the risk of decompression sickness associated with spacewalks, NASA is considering designing the next generation of exploration vehicles and habitats with a different cabin environment than used previously. The proposed environment uses a total cabin pressure of 52.7 to 58.6 kPa with an oxygen concentration of 30 to 34% by volume and was chosen with material flammability in mind. Because materials may burn differently under these conditions and there is little information on how this new environment affects the flammability of the materials onboard, it is important to conduct material flammability experiments at the intended exploration atmosphere. One method to evaluate material flammability is by its ease of ignition. To this end, piloted ignition delay tests were conducted in the Forced Ignition and Spread Test (FIST) apparatus subject to this new environment. In these tests, polymethylmethacylate (PMMA) was exposed to a range of oxidizer flow velocities and externally applied heat fluxes. The ultimate goal is to determine the individual effect of pressure and the combined effect of pressure and oxygen concentration on the ignition delay. Tests were conducted for a baseline case of normal pressure and oxygen concentration, low pressure (58.6 kPa) with normal oxygen (21%). Future work will focus on low pressure with 32% oxygen concentration (space exploration atmosphere - SEA) conditions. It was found that reducing the pressure while keeping the oxygen concentration at 21% reduced the ignition time by 17% on average. It was also noted that the critical heat flux for ignition decreases in low-pressure conditions. Because tests conducted in standard atmospheric conditions will underpredict the flammability of materials intended for use on spacecraft, fire safety onboard at exploration atmospheres may be compromised.

McAllister, Sara↗

Experimental Heat Transfer Supporting Simulated Water Well Performance on Mars

Favorable indications of massive quantities of water on Mars have initiated studies of potential changes to human Mars missions. Using a technique known as a Rodriguez Well to melt the ice, store the resulting water in a subsurface ice cavity until needed, and then pump water to the surface for use is one potential means to effect these changes. A computer simulation of the Rodriguez Well in a terrestrial environment is one of the engineering tools being used to characterize the performance of this type of well on Mars. An experiment at the NASA Johnson Space Center is gathering data for convective heat transfer and evaporation rates at Mars surface conditions so that this computer simulation can be properly modified to predict performance on Mars. While quantitative results await processing, tests have indicated that a pool of water can be maintained at 1°C to 2° C while at Mars surface temperatures and pressures.

Hoffman, Stephen J.↗

Mars Water Well Performance: Experimental Heat Transfer Results Supporting Simulations

Favorable indications of massive quantities of water on Mars have initiated studies of potential changes to human Mars missions. Using a technique known as a Rodriguez Well to melt the ice, store the resulting water in a subsurface ice cavity until needed, and then pump water to the surface for use is one potential means to effect these changes. A computer simulation of the Rodriguez Well in a terrestrial environment is one of the engineering tools being used to characterize the performance of this type of well on Mars. An experiment at the NASA Johnson Space Center is gathering data for convective heat transfer and evaporation rates at Mars surface conditions so that this computer simulation can be properly modified to predict performance on Mars. While quantitative results await processing, tests have indicated that a pool of water can be maintained at 1°C to 2° C while at Mars surface temperatures and pressures.

Mars↗

Cardiovascular examinations and observations of deconditioning during the Space Shuttle orbital flight test program

During the first four flights of the Space Shuttle, cardiovascular data were obtained on each crewmember as part of the operational medicine requirements for crew health and safety. From monitoring blood pressure and electrocardiographic data, it was possible to estimate the degree of deconditioning imposed by exposure to the microgravity environment. For this purpose, a quantitative cardiovascular index of deconditioning (CID) was derived to aid the clinician in his assessment. Isotonic saline was then investigated as a countermeasure against orthostatic intolerance and found to be effective in partially reversing the hemodynamic consequences. It was observed that the space flight environment of reentry might potentially be arrhythmogenic in at least one individual.

Bungo, M. W.↗

Aerothermal Effects of Cavities and Protuberances for High-Speed Sample Return Capsules

Extraterrestrial sample return is a growing component of solar system exploration. Currently, four missions, Stardust, 1 Muses-C, 2 Genesis, and Mars Sample Return, are under development that employ sample return as a prime component of the mission architecture. Respectively, these missions will return samples from the tail of a comet, an asteroid, the solar wind, and, Mars. An important component of these missions and the focus of this paper is the design of the sample return capsule (SRC). The purpose of the SRC is to safely return to Earth any gathered samples for terrestrial analysis. The two major design constraints for any SRC are as follows: 1) it must be able to survive a high-speed Earth entry (11 km/s to as a high as 15 km/s), 2) the mass of the SRC must be as small as possible. Because the SRC mass is carried from Earth to the sample sight and back, the SRC mass is a strong driver in the mission mass budget. Further, for the Mars Sample Return Capsule, planetary protection is another constraint. For this capsule, the probability of planetary contamination at Earth due to an SRC failure at entry must be minimal. For an SRC, a possible failure mechanism is severe local heating as a result of cavities and or protuberances in the SRC forebody heatshield. For example, the Apollo Command Module had a number of cavities and protuberances as part of the baseline designs Wind-tunnel tests of models containing small cavities and protuberances showed severe local heating augmentations in the vicinity of these surface discontinuities.4-5 As another example, the Genesis SRC forebody heat-shield contains penetrations (cavities) to mount the vehicle to the carrier bus. It is expected that these penetrations will also experience a severe local heating environment. A concern is that the large thermal gradients may produce sufficient thermal stress to cause local mechanical failure of the heatshield. Penetrations to the forebody heat-shield can also result from damage at vehicle integration, during launch, or during transportation of the sample return capsule from earth to the sample site and back. For example, the Starting SRC was damaged near the shoulder during the heatshield integration process producing a local surface discontinuity. Also, the Starting SRC traverses through the tail of a comet and is in space for 7 years. Thus, damage to the heatshield as a result of micrometeroid impact is a concern. Finally, it is difficult to characterize the effects of these potential heatshield singularities with ground-test facilities. Either detailed simulation or a dedicated flight test is required.

Olynick, David↗

Power Hibernation for Low-Cost Solar Powered Lunar Missions

Because the surface of the Moon drops to cryogenic temperatures, no solar-powered lunar spacecraft have reliably operated beyond a single lunar day. Passive thermal control cannot keep a spacecraft sufficiently warm for the 354-hour lunar night, and active thermal control requires a dramatic increase in battery mass at the expense of payload mass. Extreme conditions seen on the lunar surface suggest a radioisotope solution is ideal, but mass, cost, and schedule are inconsistent with low-cost frequent flight intent of the commercial lunar payload services (CLPS) program. To solve the issue of lunar night survivability without radioisotope sources of power and heat, a lunar power hibernation approach is being developed at the Glenn Research Center, which exploits the ability of common 18650 Lithium-ion cells to passively survive cryogenic freeze-thaw cycles and recover without apparent performance degradation. A key aspect of this hibernation approach is the use of cryogenically operable electronics that safely manage the restoration of the battery thermal environment at lunar dawn. A spacecraft utilizing this strategy will operate into the lunar night on batteries until the state of charge or spacecraft temperature reaches a predetermined threshold. At this point, systems are shut down and the battery is isolated from the main bus to prevent charge or discharge during the freezing and thawing transitions. The system remains passive until lunar dawn, where temperatures can reach as low as 50 K. All electronics must be tolerant to these conditions. When the solar arrays are finally illuminated at lunar dawn, the main bus power electronics will initiate a “cold start” and begin regulating array power. The main bus electronics must be designed to operate at cryogenic temperatures. Array power is used to warm the battery and passive electronics back to operational temperatures. Once batteries are returned to normal temperatures, diagnostics and precharging is performed, as needed, and the battery is reconnected. The overall spacecraft system reboots and returns to nominal operations until lunar night returns. To assure that we can develop batteries suited for many hibernation freeze/thaw cycles, STMD Space Technology Research Grant Program (STRG) has selected two principal investigators that will thoroughly characterize of the Li-ion cell through the freeze-thaw process, investigate degradation mechanisms, and identify potential diagnostic techniques. STMD STRG is also funding an investigation of Gallium-Nitride semiconductors for cryogenic power applications. This work includes physics-informed modeling that considers cryogenic conductivity, carrier mobility, and quantum effects that govern semiconductor performance at cryogenic temperatures. These models can enable engineers to develop accurate cryogenic simulation models that assist in the design of power controls stable over the entire lunar surface temperature range. Meanwhile, Glenn is performing cryogenic testing of batteries and electronics, establishing design guidelines for power applications in extreme cold lunar environment, and potentially developing a hibernation technology demonstrator. The hibernation approach will enable low-cost lunar robotic missions to extend their operating lifetime to many months while minimizing development costs and impact on payload capacity. The need for cryogenically operable electronics is restricted to only main bus power and battery controls, as the majority of systems simply need to passively tolerate cryogenic temperatures. This allows developers to continue to exploit the cost savings of legacy and COTS hardware with minimum modification. For these reasons, lunar power hibernation is a viable near-term solution for lunar night survivability for solar powered commercial landers.

Space power↗

The Effects of CDKN1a/p21 on Oxidative Stress and Mitochondrial Function During Long Duration Spaceflight

Spaceflight factors, such as microgravity and space radiation, are known to put astronauts at risk for negative effects to their tissue regeneration and degenerative conditions. On our previous spaceflight research, we found that long duration spaceflight produced increased oxidative stress and activation of the inflammatory response as a major contributor to physiological changes, including alterations to the cardiovascular and musculoskeletal systems, metabolism, and the liver. Proliferation, apoptosis, and ion-channel remodeling of vascular smooth muscle cells, as well as endothelial inflammation, and nitric oxide synthase-nitric oxide system regulation have been shown by spaceflight and simulated spaceflight projects. CDKN1ap21 is a regulatory gene for tissue regeneration and also plays an important role in upregulating oxidative stress caused by radiation. In further research, we have also determined CDKN1ap21 to be overexpressed during spaceflight. When knocked out in mice, however, it protects against unloading-induced changes to the morphology and physiology of carotid arteries. In this proposal, we seek to test a potential dietary countermeasure to down-regulate CDKN1ap21 in the mice and protect against oxidative stress in the rodents. Specifically, we will expose both Wildtype and CDKN1ap21-null mice to hydrogen peroxide to induce oxidative stress and observe the molecular mechanisms in which a dietary supplement protects vascular smooth muscle cells against the consequences of the apoptotic environment within Wildtype mice versus the, hypothetically, sound physiology of CDKN1ap21-null mice. The successful completion of this experiment will conclusively determine accuracy of a dietary supplement as a CDKN1ap21 suppressor. This will provide a new perspective on the problem of increased oxidative stress developing into cardiovascular disease as a result of long-term space flight. This research will address the Human Research Plan Roadmaps Risk of Cardiac Rhythm Problems including CV-8, the Risk of Cardiovascular Disease and Other Degenerative Tissue Effects from Radiation Exposure including Degen-6 and Degen-7, and the Risk of Adverse Health Event from Radiation Exposure including IM-8. Additionally, this research will address Space Biology Research Plans questions SBP CMB-1, SBP CMB-3, and SB AN-2.

Oxidative Stress↗