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NASA NTRS2007

Hinode Observations of the Onset Stage of a Solar Filament Eruption

We use Hinode X-Ray Telescope (XRT) and Solar Optical Telescope (SOT) filtergraph (FG) Stokes-V magnetogram observations, to study the early onset of a solar eruption that includes an erupting filament that we observe in TRACE EUV images. The filament undergoes a slow rise for at least 20 min prior to its fast eruption and strong soft X-ray flaring; such slow rises have been previously reported, and the new Hinode data elucidate the physical processes occurring during this period. XRT images show that during the slow-rise phase, a soft X-ray (SXR) sigmoid forms from apparent reconnection low in the sheared core field traced by the filament, and there is a low-level intensity peak in both EUV and SXRs during the slow rise. MDI and SOT FG/V magnetograms show that the pre-eruption filament is along a neutral line between opposing-polarity enhanced network cells, and the SOT magnetograms show that these opposing fields are flowing together and canceling for at least six hours prior to eruption. From the MDI data we measure the canceling network fields to be approx. 40 G, and we estimate that approx. 10(exp 19) Mx of flux canceled during the five hours prior to eruption; this is only approx. 5% of the total flux spanned by the eruption and flare, but apparently its tether-cutting cancellation was enough to destabilize the sigmoid field holding the filament and resulted in that field's eruption.

Sterling, Alphonse C.↗

NASA NTRS2012

Improved Whole-Blood-Staining Device

Dramatic improvements have been made in NASA s Whole Blood Staining Device (WBSD) since it was last described in "Whole-Blood-Staining Device," NASA Tech Briefs, Vol. 23, No. 10 (October 1999), page 64. The new system has a longer shelf life, a simpler and more effective operational procedure, improved interface with instrumentation, and shorter processing time. More specifically, the improvements have targeted bag and locking clip materials, sampling ports, and air pocket prevention. The WBSD stains whole blood collected during spaceflight for subsequent flow cytometric analysis. In short, the main device stains white blood cells by use of monoclonal antibodies conjugated to various fluorochromes, followed by lysing and fixing of the cells by use of a commercial reagent that has been diluted according to NASA safety standards. This system is compact, robust, and does not require electric power, precise mixing, or precise incubation times. Figure 1 depicts the present improved version for staining applications, which is a poly(tetrafluoroethylene) bag with a Luer-lock port and plastic locking clips. An InterLink (or equivalent) intravenous- injection port screws into the Luer-lock port. The inflatable/collapsible nature of the bag facilitates loading and helps to minimize the amount of air trapped in the fully loaded bag. Some additional uses have been identified for the device beyond whole blood staining. The WBSD has been configured for functional assays that require culture of live cells by housing sterile culture media, mitogens, and fixatives prior to use [Figure 2(a)]. Simple injection of whole blood allows cell-stimulation culture to be performed in reduced gravity conditions, and product stabilization prior to storage, while protecting astronauts from liquid biohazardous materials. Also, the improved WBSD has reconstituted powdered injectable antibiotics by mixing them with diluent liquids [Figure 2(b)]. Although such mixing can readily be performed on Earth by shaking in glass vials, it cannot readily be performed this way in outer space without entraining air bubbles. The present device can be preloaded with the powder and diluent(s) in separate compartments. The powder and diluent( s) can be mixed, without introducing air bubbles, by removing the clip(s), then shaking. This use of the device could also be advantageous in terrestrial applications because it maintains the isolation of the constituents until the time of use.

Sams, Clarence F.↗

NASA NTRS2011

Merging for Particle-Mesh Complex Particle Kinetic Modeling of the Multiple Plasma Beams

We suggest a merging procedure for the Particle-Mesh Complex Particle Kinetic (PMCPK) method in case of inter-penetrating flow (multiple plasma beams). We examine the standard particle-in-cell (PIC) and the PMCPK methods in the case of particle acceleration by shock surfing for a wide range of the control numerical parameters. The plasma dynamics is described by a hybrid (particle-ion-fluid-electron) model. Note that one may need a mesh if modeling with the computation of an electromagnetic field. Our calculations use specified, time-independent electromagnetic fields for the shock, rather than self-consistently generated fields. While a particle-mesh method is a well-verified approach, the CPK method seems to be a good approach for multiscale modeling that includes multiple regions with various particle/fluid plasma behavior. However, the CPK method is still in need of a verification for studying the basic plasma phenomena: particle heating and acceleration by collisionless shocks, magnetic field reconnection, beam dynamics, etc.

Lipatov, Alexander S.↗

NASA NTRS2012

Reproducing the Photospheric Magnetic Field Evolution during the Rise of Cycle 24 with Flux Transport by Supergranules

We simulate the transport of magnetic flux in the Sun s photosphere by an evolving pattern of cellular horizontal flows (supergranules). Characteristics of the simulated flow pattern can match observed characteristics including the velocity power spectrum, cell lifetimes, and cell motions in longitude and latitude. Simulations using an average, and north-south symmetric, meridional motion of the cellular pattern produce polar magnetic fields that are too weak in the North and too strong in the South. Simulations using cellular patterns with meridional motions that evolve with the observed changes in strength and north-south asymmetry will be analyzed to see if they reproduce the polar field evolution observed during the rise of Cycle 24.

Hathaway, David↗

NASA NTRS2009

Inventions Utilizing Microfluidics and Colloidal Particles

Several related inventions pertain to families of devices that utilize microfluidics and/or colloidal particles to obtain useful physical effects. The families of devices can be summarized as follows: (1) Microfluidic pumps and/or valves wherein colloidal-size particles driven by electrical, magnetic, or optical fields serve as the principal moving parts that propel and/or direct the affected flows. (2) Devices that are similar to the aforementioned pumps and/or valves except that they are used to manipulate light instead of fluids. The colloidal particles in these devices are substantially constrained to move in a plane and are driven to spatially order them into arrays that function, variously, as waveguides, filters, or switches for optical signals. (3) Devices wherein the ultra-laminar nature of microfluidic flows is exploited to effect separation, sorting, or filtering of colloidal particles or biological cells in suspension. (4) Devices wherein a combination of confinement and applied electrical and/or optical fields forces the colloidal particles to become arranged into three-dimensional crystal lattices. Control of the colloidal crystalline structures could be exploited to control diffraction of light. (5) Microfluidic devices, incorporating fluid waveguides, wherein switching of flows among different paths would be accompanied by switching of optical signals.

Marr, David W.↗

NASA NTRS2005

Solid Oxide Fuel Cell/Gas Turbine Hybrid Cycle Technology for Auxiliary Aerospace Power

A notional 440 kW auxiliary power unit has been developed for 300 passenger commercial transport aircraft in 2015AD. A hybrid engine using solid-oxide fuel cell stacks and a gas turbine bottoming cycle has been considered. Steady-state performance analysis during cruise operation has been presented. Trades between performance efficiency and system mass were conducted with system specific energy as the discriminator. Fuel cell performance was examined with an area specific resistance. The ratio of fuel cell versus turbine power was explored through variable fuel utilization. Area specific resistance, fuel utilization, and mission length had interacting effects upon system specific energy. During cruise operation, the simple cycle fuel cell/gas turbine hybrid was not able to outperform current turbine-driven generators for system specific energy, despite a significant improvement in system efficiency. This was due in part to the increased mass of the hybrid engine, and the increased water flow required for on-board fuel reformation. Two planar, anode-supported cell design concepts were considered. Designs that seek to minimize the metallic interconnect layer mass were seen to have a large effect upon the system mass estimates.

Steffen, Christopher J., Jr.↗

NASA NTRS2012

Subsurface Supergranular Vertical Flows as Measured Using Large Distance Separations in Time-Distance Helioseismology

As large-distance rays (say, 10 - 24deg) approach the solar surface approximately vertically, travel times measured from surface pairs for these large separations are mostly sensitive to vertical flows, at least for shallow flows within a few Mm of the solar surface. All previous analyses of supergranulation have used smaller separations and have been hampered by the difficulty of separating the horizontal and vertical flow components. We find that the large-separation travel times associated with supergranulation cannot be studied using the standard phase-speed filters of time-distance helioseismology. These filters, whose use is based upon a refractive model of the perturbations, reduce the resultant travel time signal by at least an order of magnitude at some distances. More effective filters are derived. Modeling suggests that the center-annulus travel-time difference [outward-going time minus inward-going time] in the separation range delta= 10 - 24deg is insensitive to the horizontally diverging flow from the centers of the supergranules and should lead to a constant signal from the vertical flow. Our measurement of this quantity, 5.1+/-0.1 seconds, is constant over the distance range. This magnitude of the signal cannot be caused by the level of upflow at cell centers seen at the photosphere of 10 ms(exp−1) extended in depth. It requires the vertical flow to increase with depth. A simple Gaussian model of the increase with depth implies a peak upward flow of 240 ms(exp−1) at a depth of 2.3 Mm and a peak horizontal flow of 700 ms(exp−1) at a depth of 1.6 Mm.

convection↗

NASA NTRS1997

Improvement in Suppression of Pulsed Nd:YAG Laser Light With Iodine Absorption Cells for Filtered Rayleigh Scattering Measurements

Filtered Rayleigh scattering using iodine absorption cells is an effective technique for obtaining density, temperature, and velocity measurements in high speed confined flows. By tuning a single frequency laser to a strong iodine absorption line, stray scattered laser light can be greatly suppressed. For example, the minimum transmission predicted by an iodine absorption model calculation is less than 10(exp -5) at the 18788.44/cm line using a 200 mm absorption cell containing iodine vapor at 0.46 T. Measurements obtained by other researches using a CW Nd:YAG laser agree with the model calculations. However, measurements made by us and by others using Q-switched, injection-seeded, frequency doubled Nd:YAG lasers only show minimum transmission of about 3 x 10(exp -3). This greatly reduces the applicability of the filtered Rayleigh scattering technique using these lasers in experiments having large amounts of stray scattered laser light. The purposes of the present study are to characterize the spectrum of the excess light transmitted by the iodine cell and to make changes to the laser to reduce the transmitted laser light. Transmission data as a function of laser frequency for the iodine absorption line at 18788.44/cm are presented. A planar mirror Fabry-Perot interferometer was used to characterize the frequency spectrum of the light passed through the cell. Measurements taken with the laser tuned to the center of the iodine absorption line show the light transmitted through the iodine cell to have a component with a bandwidth of about 40 GHz. This is probably caused by other modes in the laser that exist in spite of the single frequency injection beam. A second broadband component was also observed, possibly caused by the laser flash lamps or by fluorescence. An intracavity etalon was installed in the laser oscillator cavity to suppress the 40 GHz component. Measurements taken with the etalon tuned to the injection frequency showed a reduction in the transmitted laser light. This improvement allows the iodine cell to block significantly more of the stray laser light in filtered Rayleigh scattering experiments. Examples are given of filtered Rayleigh scattering measurements showing the effect of the etalon on measurements taken in a Mach 3 flow in the NASA Lewis 4 inch by 10 inch supersonic wind tunnel.

Seasholtz, Richard G.↗

NASA NTRS2009

Integrated High Payoff Rocket Propulsion Technology (IHPRPT) SiC Recession Model

SiC stability and recession rates were modeled in hydrogen/oxygen combustion environments for the Integrated High Payoff Rocket Propulsion Technology (IHPRPT) program. The IHPRPT program is a government and industry program to improve U.S. rocket propulsion systems. Within this program SiC-based ceramic matrix composites are being considered for transpiration cooled injector faceplates or rocket engine thrust chamber liners. Material testing under conditions representative of these environments was conducted at the NASA Glenn Research Center, Cell 22. For the study described herein, SiC degradation was modeled under these Cell 22 test conditions for comparison to actual test results: molar mixture ratio, MR (O2:H2) = 6, material temperatures to 1700 C, combustion gas pressures between 0.34 and 2.10 atm, and gas velocities between 8,000 and 12,000 fps. Recession was calculated assuming rates were controlled by volatility of thermally grown silica limited by gas boundary layer transport. Assumptions for use of this model were explored, including the presence of silica on the SiC surface, laminar gas boundary layer limited volatility, and accuracy of thermochemical data for volatile Si-O-H species. Recession rates were calculated as a function of temperature. It was found that at 1700 C, the highest temperature considered, the calculated recession rates were negligible, about 200 m/h, relative to the expected lifetime of the material. Results compared favorably to testing observations. Other mechanisms contributing to SiC recession are briefly described including consumption of underlying carbon and pitting. A simple expression for liquid flow on the material surface was developed from a one-dimensional treatment of the Navier-Stokes Equation. This relationship is useful to determine under which conditions glassy coatings or thermally grown silica would flow on the material surface, removing protective layers by shear forces. The velocity of liquid flow was found to depend on the gas velocity, the viscosity of gas and liquid, as well as the thickness of the gas boundary layer and the liquid layer. Calculated flow rates of a borosilicate glass coating compared well to flow rates observed for this coating tested on a SiC panel in Cell 22.

Opila, E. J.↗

NASA NTRS1998

Protein Crystal Growth Under Forced Solution Flow: Experimental Setup and General Response of Lysozyme

We have experimentally studied the effects of solution flow on the growth kinetics of the protein lysozyme. To this end, we have expanded our interferometry setup by a novel crystallization cell and solution recirculation system. This combination permits monitoring of interface morphology and kinetics with a depth resolution of 200 A at bulk flow rates of up to 2000 micron/s. Particular attention was paid to the prevention of protein denaturation that is often associated with the pumping of protein solutions. We found that at bulk flow rates it less than 250 microns/s the average growth rate and step velocity, R(sub avg) and upsilon(sub avg) increase with increasing it. This can be quantitatively understood in terms of the enhanced, convective solute supply to the interface. With high-purity solutions, it u greater than 250 microns/s lead to growth deceleration, and, at low supersaturations sigma, to growth cessation. When solutions containing approx. 1% of other protein impurities were used, growth deceleration occurred at any u greater than 0 and cessation in the low sigma experiments was reached at about half the it causing cessation with pure solution. The flow-induced changes in R(sub avg) and upsilon(sub avg) including growth cessation, were reversible and reproducible, independent of the direction of the u-changes and solution purity. Hence, we attribute the deceleration to the convection-enhanced supply of impurities to the interface, which at higher flow rates overpowers the effects of enhanced interfacial solute concentration. Most importantly, we found that convective transport leads to a significant reduction in kinetics fluctuations, in agreement with our earlier expectations for the lysozyme system. This supports our hypothesis that these long-term fluctuations represent an intrinsic response feature of the coupled bulk transport-interfacial kinetics system in the mixed growth control regime.

Vekilov, P. G.↗

NASA NTRS1988

The endocrine system in space flight

A trial natriuretic factor (ANF), a hormone recently shown to regulate sodium and water excretion, has been measured in blood specimens obtained during flight. After 30 or 42 h of weightlessness, mean ANF was elevated. After 175 or 180 h, ANF has increased by 59 percent, and it changed little between that time and soon after landing. There is probably an increase in ANF early inflight associated with the fluid shift, followed by a compensatory decrease in blood volume. Increased renal blood flow may cause the later ANF decrease. Erythropoietin (Ep), a hormone involved in the control of red blood cell proudction, was measured in blood samples taken during the first Spacelab mission and was significantly decreased on the second day of flight, suggesting also an increase in renal blood flow. Spacelab-2 investigators report that the active vitamin D metabolite 1 alpha, 25-dihydroxyvitamin D-3 increased early in the flight, indicating that a stimulus for increased bone resorption occurs by 30 h after launch.

Leach, C. S.↗

NASA NTRS2002

Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina

In albino mammals, lack of pigment in the retinal pigment epithelium is associated with retinal defects, including poor visual acuity from a photoreceptor deficit in the central retina and poor depth perception from a decrease in ipsilaterally projecting retinal fibers. Possible contributors to these abnormalities are reported delays in neuronogenesis (Ilia and Jeffery, 1996) and retinal maturation (Webster and Rowe, 1991). To further determine possible perturbations in neuronogenesis and/or differentiation, we used cell-specific markers and refined birth dating methods to examine these events during retinal ganglion cell (RGC) genesis in albino and pigmented mice from embryonic day 11 (E11) to E18. Our data indicate that relative to pigmented mice, more ganglion cells are born in the early stages of neuronogenesis in the albino retina, although the initiation of RGC genesis in the albino is unchanged. The cellular organization of the albino retina is perturbed as early as E12. In addition, cell cycle kinetics and output along the nasotemporal axis differ in retinas of albino and pigmented mice, both absolutely, with the temporal aspect of the retina expanded in albino, and relative to the position of the optic nerve head. Finally, blocking melanin synthesis in pigmented eyecups in culture leads to an increase in RGC differentiation, consistent with a role for melanin formation in regulating RGC neuronogenesis. These results point to spatiotemporal defects in neuronal production in the albino retina, which could perturb expression of genes that specify cell fate, number, and/or projection phenotype.

NASA Discipline Developmental Biology↗

NASA NTRS2000

Decreased NK-Cell Cytotoxicity after Short Flights on the Space Shuttle

Cytotoxic activity of natural killer (NK) cells and cell surface marker expression of peripheral blood mononuclear cells (PBMCs) isolated from 11 U.S. astronauts on two different missions were determined before and after 9 or 10 days of spaceflight aboard the space shuttle. Blood samples were collected 10 and 3 days before launch, within 3 hours after landing, and 3 days after landing. All PBMC preparations were cryopreserved and analyzed simultaneously in a 4-hour cytotoxicity "Cr-release assay using NK-sensitive K-562 target cells. Compared to preflight values, NK-cell cytotoxicity (corrected for lymphopenia observed on landing day) was significantly decreased at landing (P < 0.0125). It then apparently began to recover and approached preflight values by 3 days after landing. Consistent with decreased NK-cell cytotoxicity, significant increases from preflight values were found in plasma adrenocorticotropic hormone at landing. Plasma and urinary cortisol levels did not change significantly from preflight values. Expression of major lymphocyte surface markers (CD3, CD4, CD8, CD14, CD16, CD56), determined by flow cytometric analysis, revealed no consistent phenotypic changes in relative percent of NK or other lymphoid cells after 10 days of spaceflight.

Mehta, Satish K.↗

NASA NTRS1992

Overview of the relevant CFD work at Thiokol Corporation

An in-house developed proprietary advanced computational fluid dynamics code called SHARP (Trademark) is a primary tool for many flow simulations and design analyses. The SHARP code is a time dependent, two dimensional (2-D) axisymmetric numerical solution technique for the compressible Navier-Stokes equations. The solution technique in SHARP uses a vectorizable implicit, second order accurate in time and space, finite volume scheme based on an upwind flux-difference splitting of a Roe-type approximated Riemann solver, Van Leer's flux vector splitting, and a fourth order artificial dissipation scheme with a preconditioning to accelerate the flow solution. Turbulence is simulated by an algebraic model, and ultimately the kappa-epsilon model. Some other capabilities of the code are 2-D two-phase Lagrangian particle tracking and cell blockages. Extensive development and testing has been conducted on the 3-D version of the code with flow, combustion, and turbulence interactions. The emphasis here is on the specific applications of SHARP in Solid Rocket Motor design. Information is given in viewgraph form.

Chwalowski, Pawel↗

NASA NTRS2023

T-Cell Shenanigans: The Impact of MHC Pathway & Lipid Metabolism Genes on T-Cell Differentiation in the Thymus

Astronaut health and proper immune function are key to the success and sustainability of long term missions in space. While previous studies have observed adaptive immune dysfunction such as diminished WBC counts and cytokine production during spaceflight, the underlying mechanisms behind why immune function worsens are poorly understood. Using transcriptomic data analyzed from mouse thymus tissues available from the GLDS-421 experiment on the Rodent Research-9 mission, we used the GeneLab standardized RNA-Seq pipeline and identified four genes that were largely dysregulated (p<0.05): lipid metabolic genes Hpgd and Pgr and cell cycle regulator genes Cenpe and Kif11. We analyzed how these genes regulate the MHC-TCR interaction, and used this understanding to propose a novel mechanism for alterations to T-cell differentiation. In our experimental methodology, we propose to use 36 transgenic CD4-CD8 mice and 12 wild-type mice from the Jackson Lab to create a microgravity-simulating hindlimb unloading model and test our hypothesis. By silencing the aforementioned genes using custom siRNA based primers, we will use flow cytometry and RNA-Seq to quantify Treg counts in comparison to naive CD4+ cells, Alamar Blue assay for observing the metabolic activity in T-cells, and Enzyme-Linked Immunosorbent Assay (ELISA) to observe the general immune response in terms of T-cell differentiation. We hope this methodology will improve our knowledge of MHC-TCR interaction, contribute to a better understanding of the mechanisms of autoimmune diseases such as lupus, and improve the success rate of newer cancer treatments such as CAR-T cell therapy.

GL4HS↗

NASA NTRSDate not supplied

Single-Cell Analysis of Yeast (Saccharomyces cerevisiae) Using Hydrogel Encapsulation

Space radiation poses a major health risk to astronauts. To fulfill NASA’s mission of exploration beyond Earth, the biological effects of Galactic Cosmic Radiation and gamma radiation must be investigated to elucidate cellular damage mechanisms and inform countermeasure protocols to safely bring humans beyond Earth’s magnetosphere. Budding yeast (Saccharomyces cerevisiae) are commonly used in experiments as a model organism for studying the effects of radiation on eukaryotes. Radiobiology of yeast at the single cell level is poorly understood, yet crucial for informing models to aid in the design and interpretation of experiments. We are using a novel method of microencapsulation in hydrogel particles (PicoShells) to enable analysis of the distribution of radiation-induced damage among yeast cells at the single-cell level, in high throughput. Here we describe the development of methods for culturing, visualization, and quantification of encapsulated yeast. The encapsulated yeast are cultured in Yeast extract-Peptone-Dextrose (YPD) medium, fixed in formaldehyde or ethanol, and stained with DAPI or propidium iodide, then visualized using microscopy or enumerated using flow cytometry, with the aim of developing a protocol to enumerate the distribution of viable cells in each PicoShell. This will allow us to quantify how different forms of radiation can generate different distributions of damage across a population of cells, ultimately providing insight into the biological effects of space-relevant ionizing radiation.

yeast↗

NASA NTRS1984

Experimental investigation of shock-cell noise reduction for dual-stream nozzles in simulated flight

Six scale-model nozzles were tested in an anechoic facility to evauate the effectiveness of convergent-divergent (C-D) terminations in reducing shock-cell noise of unsuppressed and mechanically suppressed coannular plug nozzles. One hundred fifty-three acoustic test points with inverted velocity profiles were conducted under static and simulated flight conditions. Diagnostic flow visualization with a shadowgraph and velocity measurements with a laser velocimeter were performed on selected plumes. Shock-cells were identified on the plug and downstream of the plug of the unsuppressed convergent coannular nozzle with truncated plug. Broadband peak frequencies predicted with the two shock-cell structures were correlated with the observed spectra using the measured shock-cell spacings. Relative to a convergent circular nozzle, the perceived noise level (PNL) data at an observer angle of 60 deg relative to inlet, indicated a reduction of (1) 6.5 dB and 9.2 dB with unsuppressed C-D coannular nozzle with truncated plug and (2) 7.7 dB and 8.3 dB with suppressed C-D coannular nozzle under static and simulated flight conditions, espectively. The unsuppressed C-D coannular nozzle with truncated plug, operating at the C-D design condition, had shock-cells downstream of the plug with no shock-cells on the plug. The downstream shock-cells were eliminated by replacing the truncated plug with a smooth extension to obtain an additional 2.4 dB and 3 dB front quadrant PNL reduction, under static and simulated flight conditions, respectively. Other results are discussed.

Janardan, B. A.↗

NASA NTRS1990

Three-dimensional compressible convection at low Prandtl numbers

Numerical simulations are used to study fully compressible thermal convection at large Rayleigh numbers. Results are presented from a sequence of three-dimensional simulations that reveal a transition from gradually-evolving laminar convection to nearly turbulent convection as the Prandtl number is reduced from a value of unity to one-tenth. The convective flows form irregular cellular patterns near the upper surface, possesing a network of fast downflow at cell peripheries and gentler upflow at cell centers. At greater depths the curving sheets of downflow collapse into plumes which may twist and possess substantial vertical vorticity. For the lowest Prandtl number, the convection near the bottom of the layer appears to be turbulent, yet the rapidly varying small-scale flow structure there is accompanied by more ordered sites of wavering upflow, with the latter able to penetrate all the way to the upper boundary. Thus a significant component of the flow is able to extend over multiple density scale heights, in contrast to what is argued in formulating mixing-length models for stellar convection. Results are also shown from two-dimensional simulations carried out with very high spatial resolution, which reveal that supersonic convection with fluttering shock systems can be realized.

Toomre, Juri↗