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At least 811 records · Page 45
All sourcesNASA NTRSDOE OSTI
NASA NTRS2009

Circuits Enhance Scientific Instruments and Safety Devices

Since its founding in 1958, NASA has pioneered the use of different frequencies on the electromagnetic spectrum - including X-ray, microwave, and infrared wavelengths - to gather information about distant celestial bodies. During the 1962 Mariner 2 mission, NASA used microwave radiometers that operated in the range of 15-23 gigahertz (GHz) to assess the surface temperature of Venus and to determine the percentage of water vapor in its atmosphere. Today, there is another area on the spectrum proving uniquely useful to scientists: the terahertz (THz) range, spanning from about 100 GHz-10,000 GHz. (1 THz equals approximately 1,000 GHz.) Terahertz frequencies span the lesser-known gap on the electromagnetic spectrum between microwave radiation and infrared (and visible) light, falling within the spectral range where most simple molecules resonate. This molecular resonance makes terahertz particularly useful for chemical spectroscopy and the remote sensing of specific molecules. In the 1990s, NASA began using frequencies above 300 GHz (more than an order of magnitude higher than the instrumentation on Mariner 2) to perform spectral analysis of molecular clouds and planetary atmospheres. Instruments using these higher frequencies have included the Microwave Limb Sounder (MLS) on the Upper Atmosphere Research Satellite (UARS), deployed from 1991-2001, and the Microwave Instrument for the Rosetta Orbiter (MIRO), launched in 2004. With UARS-MLS, NASA used advanced terahertz receivers to measure the emission signatures from atmospheric molecules, providing researchers with valuable data about the changes in the Earth s protective ozone layer. MIRO, set to rendezvous with the comet 67P Churyumov-Gerasimenko in 2014, will use terahertz instrumentation to analyze the comet s dust and gases. Although NASA has been a driving force behind the development of terahertz technology, scientific equipment for terahertz research - including transmitters, receivers, and basic test and measurement equipment - is not widely available, making scientific experiments in this range between traditional electronics and quantum photonics more costly and greatly limiting commercial development in the field. Given NASA s interest in studying distant bodies in space as well as in improving life on Earth, the Agency has collaborated with private industry to develop terahertz technologies.

Source record↗
NASA NTRS2006

Ion-Exclusion Chromatography for Analyzing Organics in Water

A liquid-chromatography technique has been developed for use in the quantitative analysis of urea (and of other nonvolatile organic compounds typically found with urea) dissolved in water. The technique involves the use of a column that contains an ion-exclusion resin; heretofore, this column has been sold for use in analyzing monosaccharides and food softeners, but not for analyzing water supplies. The prior technique commonly used to analyze water for urea content has been one of high-performance liquid chromatography (HPLC), with reliance on hydrophobic interactions between analytes in a water sample and long-chain alkyl groups bonded to an HPLC column. The prior technique has proven inadequate because of a strong tendency toward co-elution of urea with other compounds. Co-elution often causes the urea and other compounds to be crowded into a narrow region of the chromatogram (see left part of figure), thereby giving rise to low chromatographic resolution and misidentification of compounds. It is possible to quantitate urea or another analyte via ultraviolet- and visible-light absorbance measurements, but in order to perform such measurements, it is necessary to dilute the sample, causing a significant loss of sensitivity. The ion-exclusion resin used in the improved technique is sulfonated polystyrene in the calcium form. Whereas the alkyl-chain column used in the prior technique separates compounds on the basis of polarity only, the ion-exclusion-resin column used in the improved technique separates compounds on the basis of both molecular size and electric charge. As a result, the degree of separation is increased: instead of being crowded together into a single chromatographic peak only about 1 to 2 minutes wide as in the prior technique, the chromatographic peaks of different compounds are now separated from each other and spread out over a range about 33 minutes wide (see right part of figure), and the urea peak can readily be distinguished from the other peaks. Although the analysis takes more time in the improved technique, this disadvantage is offset by two important advantages: Sensitivity is increased. The minimum concentration of urea that can be measured is reduced (to between 1/5 and 1/3 of that of the prior technique) because it is not necessary to dilute the sample. The separation of peaks facilitates the identification and quantitation of the various compounds. The resolution of the compounds other than urea makes it possible to identify those compounds by use of mass spectrometry.

Sauer, Richard↗
NASA NTRS2006

Using Quasiparticle Poisoning To Detect Photons

According to a proposal, a phenomenon associated with excitation of quasiparticles in certain superconducting quantum devices would be exploited as a means of detecting photons with exquisite sensitivity. The phenomenon could also be exploited to perform medium-resolution spectroscopy. The proposal was inspired by the observation that Coulomb blockade devices upon which some quantum logic gates are based are extremely sensitive to quasiparticles excited above the superconducting gaps in their leads. The presence of quasiparticles in the leads can be easily detected via the charge states. If quasiparticles could be generated in the leads by absorption of photons, then the devices could be used as very sensitive detectors of electromagnetic radiation over the spectral range from x-rays to submillimeter waves. The devices in question are single-Cooper-pair boxes (SCBs), which are mesoscopic superconducting devices developed for quantum computing. An SCB consists of a small superconducting island connected to a reservoir via a small tunnel junction and connected to a voltage source through a gate capacitor. An SCB is an artificial two-level quantum system, the Hamiltonian of which can be controlled by the gate voltage. One measures the expected value of the charge of the eigenvectors of this quantum system by use of a radio-frequency single-electron transistor. A plot of this expected value of charge as a function of gate voltage resembles a staircase that, in the ideal case, consists of steps of height 2 e (where e is the charge of one electron). Experiments have shown that depending on the parameters of the device, quasiparticles in the form of "broken" Cooper pairs present in the reservoir can tunnel to the island, giving rise to steps of 1 e. This effect is sometimes called "poisoning." Simulations have shown that an extremely small average number of quasiparticles can generate a 1-e periodic signal. In a device according to the proposal, this poisoning would be turned to advantage. Depending on the wavelength, an antenna or other component would be used to couple radiation into the reservoir, wherein the absorption of photons would break Cooper pairs, thereby creating quasiparticles that, in turn, would tunnel to the island, creating a 1-e signal. On the basis of conservative estimates of device parameters derived from experimental data and computational simulations that fit the data, it has been estimated that the noise equivalent power of a device according to the proposal could be as low as 6 10(exp -22) W/Hz(exp 1/2). It has also been estimated that the spectroscopic resolution (photon energy divided by increment of photon energy) of such a device in visible light would exceed 100.

Echternach, Pierre↗
NASA NTRS2010

Miniaturized Environmental Scanning Electron Microscope for In Situ Planetary Studies

The exploration of remote planetary surfaces calls for the advancement of low power, highly-miniaturized instrumentation. Instruments of this nature that are capable of multiple types of analyses will prove to be particularly useful as we prepare for human return to the moon, and as we continue to explore increasingly remote locations in our Solar System. To this end, our group has been developing a miniaturized Environmental-Scanning Electron Microscope (mESEM) capable of remote investigations of mineralogical samples through in-situ topographical and chemical analysis on a fine scale. The functioning of an SEM is well known: an electron beam is focused to nanometer-scale onto a given sample where resulting emissions such as backscattered and secondary electrons, X-rays, and visible light are registered. Raster scanning the primary electron beam across the sample then gives a fine-scale image of the surface topography (texture), crystalline structure and orientation, with accompanying elemental composition. The flexibility in the types of measurements the mESEM is capable of, makes it ideally suited for a variety of applications. The mESEM is appropriate for use on multiple planetary surfaces, and for a variety of mission goals (from science to non-destructive analysis to ISRU). We will identify potential applications and range of potential uses related to planetary exploration. Over the past few of years we have initiated fabrication and testing of a proof-of-concept assembly, consisting of a cold-field-emission electron gun and custom high-voltage power supply, electrostatic electron-beam focusing column, and scanning-imaging electronics plus backscatter detector. Current project status will be discussed. This effort is funded through the NASA Research Opportunities in Space and Earth Sciences - Planetary Instrument Definition and Development Program.

Gaskin, Jessica↗
NASA NTRS2010

Hybrid AlGaN-SiC Avalanche Photodiode for Deep-UV Photon Detection

The proposed device is capable of counting ultraviolet (UV) photons, is compatible for inclusion into space instruments, and has applications as deep- UV detectors for calibration systems, curing systems, and crack detection. The device is based on a Separate Absorption and Charge Multiplication (SACM) structure. It is based on aluminum gallium nitride (AlGaN) absorber on a silicon carbide APD (avalanche photodiode). The AlGaN layer absorbs incident UV photons and injects photogenerated carriers into an underlying SiC APD that is operated in Geiger mode and provides current multiplication via avalanche breakdown. The solid-state detector is capable of sensing 100-to-365-nanometer wavelength radiation at a flux level as low as 6 photons/pixel/s. Advantages include, visible-light blindness, operation in harsh environments (e.g., high temperatures), deep-UV detection response, high gain, and Geiger mode operation at low voltage. Furthermore, the device can also be designed in array formats, e.g., linear arrays or 2D arrays (micropixels inside a superpixel).

Aslam, Shahid↗
NASA NTRS2010

Process to Selectively Distinguish Viable from Non-Viable Bacterial Cells

The combination of ethidium monoazide (EMA) and post-fragmentation, randomly primed DNA amplification technologies will enhance the analytical capability to discern viable from non-viable bacterial cells in spacecraft-related samples. Intercalating agents have been widely used since the inception of molecular biology to stain and visualize nucleic acids. Only recently, intercalating agents such as EMA have been exploited to selectively distinguish viable from dead bacterial cells. Intercalating dyes can only penetrate the membranes of dead cells. Once through the membrane and actually inside the cell, they intercalate DNA and, upon photolysis with visible light, produce stable DNA monoadducts. Once the DNA is crosslinked, it becomes insoluble and unable to be fragmented for post-fragmentation, randomly primed DNA library formation. Viable organisms DNA remains unaffected by the intercalating agents, allowing for amplification via post-fragmentation, randomly primed technologies. This results in the ability to carry out downstream nucleic acid-based analyses on viable microbes to the exclusion of all non-viable cells.

LaDuc, Myron T.↗
NASA NTRS2010

Views from EPOXI. Colors in Our Solar System as an Analog for Extrasolar Planets

With extrasolar planet detection becoming more common place, the frontiers of extrasolar planet science have moved beyond detection to the observations required to determine planetary properties. Once the existing observational challenges have been overcome, the first visible-light studies of extrasolar Earth-sized planets will likely employ filter photometry or low-resolution. spectroscopy to observe disk-integrated radiation from the unresolved planet. While spectroscopy of these targets is highly desirable, and provides the most robust form of characterization. S/N considerations presently limit spectroscopic measurements of extrasolar worlds. Broadband filter photometry will thus serve as a first line of characterization. In this paper we use Extrasolar Observation and Characterization (EPOCh) filter photometry of the Earth. Moon and Mars model spectra. and previous photometric and spectroscopic observations of a range the solar system planets. Titan, and Moon to explore the limitations of using color as a baseline for understanding extrasolar planets

Crow, Carolyn A.↗
NASA NTRS2010

Achieving Global Ocean Color Climate Data Records

Ocean color, or the spectral distribution of visible light upwelling from beneath the ocean surface, carries information on the composition and concentration of biological constituents within the water column. The CZCS mission in 1978 demonstrated that quantitative ocean color measurements could be. made from spaceborne sensors, given sufficient corrections for atmospheric effects and a rigorous calibration and validation program. The launch of SeaWiFS in 1997 represents the beginning of NASA's ongoing efforts to develop a continuous ocean color data record with sufficient coverage and fidelity for global change research. Achievements in establishing and maintaining the consistency of the time-series through multiple missions and varying instrument designs will be highlighted in this talk, including measurements from NASA'S MODIS instruments currently flying on the Terra and Aqua platforms, as well as the MERIS sensor flown by ESA and the OCM-2 sensor recently launched by ISRO.

Franz, Bryan↗
NASA NTRS2006

Infrared Instrument for Detecting Hydrogen Fires

The figure shows an instrument incorporating an infrared camera for detecting small hydrogen fires. The instrument has been developed as an improved replacement for prior infrared and ultraviolet instruments used to detect hydrogen fires. The need for this or any such instrument arises because hydrogen fires (e.g., those associated with leaks from tanks, valves, and ducts) pose a great danger, yet they emit so little visible light that they are mostly undetectable by the unaided human eye. The main performance advantage offered by the present instrument over prior hydrogen-fire-detecting instruments lies in its greater ability to avoid false alarms by discriminating against reflected infrared light, including that originating in (1) the Sun, (2) welding torches, and (3) deliberately ignited hydrogen flames (e.g., ullage-burn-off flames) that are nearby but outside the field of view intended to be monitored by the instrument. Like prior such instruments, this instrument is based mostly on the principle of detecting infrared emission above a threshold level. However, in addition, this instrument utilizes information on the spatial distribution of infrared light from a source that it detects. Because the combination of spatial and threshold information about a flame tends to constitute a unique signature that differs from that of reflected infrared light originating in a source not in the field of view, the incidence of false alarms is reduced substantially below that of related prior threshold- based instruments.

Youngquist, Robert↗
NASA NTRS2010

Visible Nulling Coronagraphy Testbed Development for Exoplanet Detection

Three of the recently completed NASA Astrophysics Strategic Mission Concept (ASMC) studies addressed the feasibility of using a Visible Nulling Coronagraph (VNC) as the prime instrument for exoplanet science. The VNC approach is one of the few approaches that works with filled, segmented and sparse or diluted aperture telescope systems and thus spans the space of potential ASMC exoplanet missions. NASA/Goddard Space Flight Center (GSFC) has a well-established effort to develop VNC technologies and has developed an incremental sequence of VNC testbeds to advance the this approach and the technologies associated with it. Herein we report on the continued development of the vacuum Visible Nulling Coronagraph testbed (VNT). The VNT is an ultra-stable vibration isolated testbed that operates under high bandwidth closed-loop control within a vacuum chamber. It will be used to achieve an incremental sequence of three visible light nulling milestones of sequentially higher contrasts of 10(exp 8) , 10(exp 9) and 10(exp 10) at an inner working angle of 2*lambda/D and ultimately culminate in spectrally broadband (>20%) high contrast imaging. Each of the milestones, one per year, is traceable to one or more of the ASMC studies. The VNT uses a modified Mach-Zehnder nulling interferometer, modified with a modified "W" configuration to accommodate a hex-packed MEMS based deformable mirror, a coherent fiber bundle and achromatic phase shifters. Discussed will be the optical configuration laboratory results, critical technologies and the null sensing and control approach.

Lyon, Richard G.↗
NASA NTRS2011

Views from EPOXI: Colors in Our Solar System as an Analog for Extrasolar Planets

The first visible-light studies of Earth-sized extrasolar planets will employ photometry or low-resolution spectroscopy. This work uses EPOCh medium-hand filter photometry between 150 and 950 nm obtained with the Deep Impact (DI) High Resolution Instrument (HRI) of Earth, the Moon, and Mars in addition to previous full-disk observations of the other six solar system planets and Titan to analyze the limitations of using photometric colors to characterize extrasolar planets. We determined that the HRI 350, 550, and 850 nm filters are optimal for distinguishing Earth from the other planets and separating planets to first order based on their atmospheric and surface properties. Detailed conclusions that can be drawn about exoplanet atmospheres simply from a color-color plot are limited due to potentially competing physical processes in the atmosphere. The presence of a Rayleigh scattering atmosphere can be detected by an increase in the 350-550 nm brightness ratio, but the absence of Rayleigh scattering cannot be confirmed due to the existence of atmospheric and surface absorbing species in the UV. Methane and ammonia are the only species responsible for strong absorption in the 850 nm filter in our solar system. The combination of physical processes present on extrasolar planets may differ from those we see locally. Nevertheless, a generation of telescopes capable of collecting such photometric observations can serve a critical role in first-order characterization and constraining the population of Earth-like extrasolar planets.

Crow, Carolyn A.↗
NASA NTRS2004

Aligning Arrays of Lenses and Single-Mode Optical Fibers

A procedure now under development is intended to enable the precise alignment of sheet arrays of microscopic lenses with the end faces of a coherent bundle of as many as 1,000 single-mode optical fibers packed closely in a regular array (see Figure 1). In the original application that prompted this development, the precise assembly of lenses and optical fibers serves as a single-mode spatial filter for a visible-light nulling interferometer. The precision of alignment must be sufficient to limit any remaining wavefront error to a root-mean-square value of less than 1/10 of a wavelength of light. This wavefront-error limit translates to requirements to (1) ensure uniformity of both the lens and fiber arrays, (2) ensure that the lateral distance from the central axis of each lens and the corresponding optical fiber is no more than a fraction of a micron, (3) angularly align the lens-sheet planes and the fiber-bundle end faces to within a few arc seconds, and (4) axially align the lenses and the fiber-bundle end faces to within tens of microns of the focal distance. Figure 2 depicts the apparatus used in the alignment procedure. The beam of light from a Zygo (or equivalent) interferometer is first compressed by a ratio of 20:1 so that upon its return to the interferometer, the beam will be magnified enough to enable measurement of wavefront quality. The apparatus includes relay lenses that enable imaging of the arrays of microscopic lenses in a charge-coupled-device (CCD) camera that is part of the interferometer. One of the arrays of microscopic lenses is mounted on a 6-axis stage, in proximity to the front face of the bundle of optical fibers. The bundle is mounted on a separate stage. A mirror is attached to the back face of the bundle of optical fibers for retroreflection of light. When a microscopic lens and a fiber are aligned with each other, the affected portion of the light is reflected back by the mirror, recollimated by the microscopic lens, transmitted through the relay lenses and the beam compressor/expander, then split so that half goes to a detector and half to the interferometer. The output of the detector is used as a feedback control signal for the six-axis stage to effect alignment.

Liu, Duncan↗
NASA NTRS2011

Vacuum Nuller Testbed (VNT) Performance, Characterization and Null Control: Progress Report

Herein we report on the development. sensing and control and our first results with the Vacuum Nuller Testbed to realize a Visible Nulling Coronagraph (VNC) for exoplanet coronagraphy. The VNC is one of the few approaches that works with filled. segmented and sparse or diluted-aperture telescope systems. It thus spans a range of potential future NASA telescopes and could be Hown as a separate instrument on such a future mission. NASA/Goddard Space Flight Center (GSFC) has a well-established effort to develop VNC technologies. and has developed an incremental sequence of VNC testbeds to advance this approach and the enabling technologies associated with it. We discuss the continued development of the vacuum Visible Nulling Coronagraph testbed (VNT). Tbe VNT is an ultra-stable vibration isolated testbed that operates under closed-loop control within a vacuum chamber. It will be used to achieve an incremental sequence of three visible-light nulling milestones with sequentially higher contrasts of 10(sup 8), 10(sup 9) and ideally 10(sup 10) at an inner working angle of 2*lambda/D. The VNT is based on a modified Mach-Zehnder nulling interferometer, with a "W" configuration to accommodate a hex-packed MEMS based deformable mirror, a coherent fiber bundle and achromatic phase shifters. We discuss the initial laboratory results, the optical configuration, critical technologies and the null sensing and control approach.

Lyon, Richard G.↗
NASA NTRS2011

Development of the SOFIA Image Processing Tool

The Stratospheric Observatory for Infrared Astronomy (SOFIA) is a Boeing 747SP carrying a 2.5 meter infrared telescope capable of operating between at altitudes of between twelve and fourteen kilometers, which is above more than 99 percent of the water vapor in the atmosphere. The ability to make observations above most water vapor coupled with the ability to make observations from anywhere, anytime, make SOFIA one of the world s premiere infrared observatories. SOFIA uses three visible light CCD imagers to assist in pointing the telescope. The data from these imagers is stored in archive files as is housekeeping data, which contains information such as boresight and area of interest locations. A tool that could both extract and process data from the archive files was developed.

Adams, Alexander N.↗
NASA NTRS2012

Receivers Gather Data for Climate, Weather Prediction

Signals from global positioning system (GPS) satellites are now being used for more than just location and navigation information. By looking at the radio waves from GPS satellites, a technology developed at NASA s Jet Propulsion Laboratory (JPL) not only precisely calculates its position, but can also use a technique known as radio occultation to help scientists study the Earth s atmosphere and gravity field to improve weather forecasts, monitor climate change, and enhance space weather research. The University Corporation for Atmospheric Research (UCAR), a nonprofit group of universities in Boulder, Colorado, compares radio occultation to the appearance of a pencil when viewed though a glass of water. The water molecules change the path of visible light waves so that the pencil appears bent, just like molecules in the air bend GPS radio signals as they pass through (or are occulted by) the atmosphere. Through measurements of the amount of bending in the signals, scientists can construct detailed images of the ionosphere (the energetic upper part of the atmosphere) and also gather information about atmospheric density, pressure, temperature, and moisture. Once collected, this data can be input into weather forecasting and climate models for weather prediction and climate studies. Traditionally, such information is obtained through the use of weather balloons. In 1998, JPL started developing a new class of GPS space science receivers, called Black Jack, that could take precise measurements of how GPS signals are distorted or delayed along their way to the receiver. By 2006, the first demonstration of a GPS radio occultation constellation was launched through a collaboration among Taiwan s National Science Council and National Space Organization, the U.S. National Science Foundation, NASA, the National Oceanic and Atmospheric Administration (NOAA), and other Federal entities. Called the Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC), JPL was responsible for designing COSMIC s primary instrument - based on its revolutionary Black Jack receiver.

Source record↗
NASA NTRS2011

A Variational Method to Retrieve the Extinction Profile in Liquid Clouds Using Multiple Field-of-View Lidar

While liquid clouds playa very important role in the global radiation budget, it's been very difficult to remotely determine their internal cloud structure. Ordinary lidar instruments (similar to radars but using visible light pulses) receive strong signals from such clouds, but the information is limited to a thin layer near the cloud boundary. Multiple field-of-view (FOV) lidars offer some new hope as they are able to isolate photons that were scattered many times by cloud droplets and penetrated deep into a cloud before returning to the instrument. Their data contains new information on cloud structure, although the lack of fast simulation methods made it challenging to interpret the observations. This paper describes a fast new technique that can simulate multiple-FOV lidar signals and can even estimate the way the signals would change in response to changes in cloud properties-an ability that allows quick refinements in our initial guesses of cloud structure. Results for a hypothetical airborne three-FOV lidar suggest that this approach can help determine cloud structure for a deeper layer in clouds, and can reliably determine the optical thickness of even fairly thick liquid clouds. The algorithm is also applied to stratocumulus observations by the 8-FOV airborne "THOR" lidar. These tests demonstrate that the new method can determine the depth to which a lidar provides useful information on vertical cloud structure. This work opens the way to exploit data from spaceborne lidar and radar more rigorously than has been possible up to now.

Pounder, Nicola L.↗
NASA NTRS2012

Exploring the Birth and Evolution of the Universe: How Detectors Have Revolutionized Space Astronomy

The past century has seen tremendous advances in the capability of instruments used for astronomical imaging and spectroscopy. Capabilities of instruments have expanded in many dimensions; the scale of telescopes has grown tremendously, the wavelengths used for astronomy have grown from visible light to the full electromagnetic spectrum, extending from gamma rays to low frequency radio waves. Additional advances have been enabled by the availability of space facilities, which eliminate the effects of the earths atmosphere and magnetosphere, and allow cooling of instruments to avoid instrumental thermal radiation. Even with all these advances, the increase in capability of detection systems has produced truly revolutionary improvements in capability. Today, I will describe the advances in astronomical detection from the photographic plates of the early 20th century to the giant high efficiency focal planes being developed for modern space and ground based astronomical instrument. I will review the demanding performance requirements set by space astronomy, and show how the detector community has risen to the challenge in producing high performance detectors for the Hubble Space Telescope, the Spitzer Space Telescope, and the James Webb Space Telescope, now under development.

Moseley, Samuel H.↗
NASA NTRS2012

Molecular Technique to Reduce PCR Bias for Deeper Understanding of Microbial Diversity

Current planetary protection policies require that spacecraft targeted to sensitive solar system bodies be assembled and readied for launch in controlled cleanroom environments. A better understanding of the distribution and frequency at which high-risk contaminant microbes are encountered on spacecraft surfaces would significantly aid in assessing the threat of forward contamination. However, despite a growing understanding of the diverse microbial populations present in cleanrooms, less abundant microbial populations are probably not adequately taken into account due to technological limitations. This novel approach encompasses a wide spectrum of microbial species and will represent the true picture of spacecraft cleanroom-associated microbial diversity. All of the current microbial diversity assessment techniques are based on an initial PCR amplification step. However, a number of factors are known to bias PCR amplification and jeopardize the true representation of bacterial diversity. PCR amplification of a minor template appears to be suppressed by the amplification of a more abundant template. It is widely acknowledged among environmental molecular microbiologists that genetic biosignatures identified from an environment only represent the most dominant populations. The technological bottleneck overlooks the presence of the less abundant minority population and may underestimate their role in the ecosystem maintenance. DNA intercalating agents such as propidium monoazide (PMA) covalently bind with DNA molecules upon photolysis using visible light, and make it unavailable for DNA polymerase enzyme during polymerase chain reaction (PCR). Environmental DNA samples will be treated with suboptimum PMA concentration, enough to intercalate with 90 99% of the total DNA. The probability of PMA binding with DNA from abundant bacterial species will be much higher than binding with DNA from less abundant species. This will increase the relative DNA concentration of previously "shadowed" less abundant species available for PCR amplification. These PCR products obtained with and without PMA treatment will then be subjected to downstream diversity analyses such as sequencing and DNA microarray. It is expected that PMA-coupled PCR will amplify the "minority population" and help in understanding microbial diversity spectrum of an environmental sample at a much deeper level. This new protocol aims to overcome the major potential biases faced when analyzing microbial 16S rRNA gene diversity. This study will lead to a technological advancement and a commercial product that will aid microbial ecologists in understanding microbial diversity from various environmental niches. Implementation of this technique may lead to discoveries of novel microbes and their functions in sustenance of the ecosystem.

Vaishampayan, Parag A.↗