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At least 811 records · Page 45

Great Lakes Energy-Water Model

The nexus between thermoelectric power production and water use is not uniform across the U.S., but rather differs according to regional physiography, demography, power plant fleet composition, and the transmission network. That is, in some regions water demand for thermoelectric production is relatively small while in other regions it represents the dominate use. The later is the case for the Great Lakes region, which has important implications for the water resources and aquatic ecology of the Great Lakes watershed. This is today, but what about the future? Projected demographic trends, shifting lifestyles, and economic growth coupled with the threat of global climate change and mounting pressure for greater U.S. energy security could have profound effects on the region's energy future. Planning for such an uncertain future is further complicated by the fact that energy and environmental planning and regulatory decision-making is largely bifurcated in the region, with environmental and water resource concerns generally taken into account after new energy facilities and technologies have been proposed, or practices are already in place. Based on these confounding needs, the objective of this effort is to develop Great Lakes-specific methods and tools to integrate energy and water resource planning and thereby support the dual goals of smarter energy planning and development, and protection of Great Lakes water resources. Guiding policies for this planning are the Great Lakes and St. Lawrence River Basin Water Resources Compact and the Great Lakes Water Quality Agreement. The desired outcome of integrated energy-water-aquatic resource planning is a more sustainable regional energy mix for the Great Lakes basin ecosystem.

Tidwell, Vincent↗

Radiation measurements at ICOS ecosystem stations

Solar radiation is a prime driver of energy and carbon fluxes in natural ecosystems. Radiation measurements are essential for interpreting ecosystem scale greenhouse gases and energy fluxes as well as many other observations performed at ecosystem stations of the Integrated Carbon Observation System (ICOS). Here, we describe and explain the relevance of the radiation variables that are monitored continuously at ICOS ecosystem stations and define recommendations to perform these measurements with consistent and comparable accuracy. The measurement methodology and instruments are described including detailed technical specifications. Guidelines for instrumental set up as well as for operation, maintenance and data collection are defined considering both ICOS scientific objectives and practical operational constraints. For measurements of short-wave (solar) and long wave (infrared) radiation components, requirements for the ICOS network are based on available well-defined state-of-the art standards (World Meteorological Organization, International Organization for Standardization). For photosynthetically active radiation measurements, some basic instrumental requirements are based on the performance of commercially available sensors. Since site specific conditions and practical constraints at individual ICOS ecosystem stations may hamper the applicability of standard requirements, we recommend that ICOS develops mid-term coordinated actions to assess the effective level of uncertainties in radiation measurements at the network scale.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Microbial diversity drives carbon use efficiency in a model soil

Abstract Empirical evidence for the response of soil carbon cycling to the combined effects of warming, drought and diversity loss is scarce. Microbial carbon use efficiency (CUE) plays a central role in regulating the flow of carbon through soil, yet how biotic and abiotic factors interact to drive it remains unclear. Here, we combine distinct community inocula (a biotic factor) with different temperature and moisture conditions (abiotic factors) to manipulate microbial diversity and community structure within a model soil. While community composition and diversity are the strongest predictors of CUE, abiotic factors modulated the relationship between diversity and CUE, with CUE being positively correlated with bacterial diversity only under high moisture. Altogether these results indicate that the diversity × ecosystem-function relationship can be impaired under non-favorable conditions in soils, and that to understand changes in soil C cycling we need to account for the multiple facets of global changes.

54 ENVIRONMENTAL SCIENCES↗

Blockchain-Enabled Secure Device-to-Device Communication in Software-Defined Networking

The Internet of Things (IoT) continues to increase the demand for seamless communication among IoT devices. The rapid growth of IoT devices has led to an exponential increase in device-to-device (D2D) communication within the Software-Defined Networking (SDN), though it enables a flexible archi-tecture for managing network resources. However, traditional security models face challenges (e.g., Security, privacy, and trust) in addressing the dynamic and decentralized nature of these communications. Despite of these challenges, this paper proposes a novel approach that leverages blockchain technology to enhance the security, privacy, and trustworthiness of D2D communication within an SDN environment. The proposed approach integrates blockchain nodes in sDN components to establish a decentralized ledger for transparent and verifiable records. Smart contracts enforce authentication rules to ensure that only authenticated devices can access the network and engage in transactions securely. It also automates the security policies to ensure temper resistance execution using the cryptographic mechanism for data integrity and authentic communication. The Implementation of the proposed algorithms validates the resilience of the proposed approach against cyberattacks. Overall, the proposed approach enables efficient and secure D2D communication for resilient SDN infrastructure in IoT ecosystems.

Das, Debashis↗

Contextualizing Non-Powered Dam Site Selection for Archimedes Screw Turbines: A Methodology for Responsible Archimedes Screw Turbine Conversion at Existing Dams

Non-powered dams represent 97% of dams in the United States and their energy generation potential has not been fully realized. The use of an Archimedes screw turbine to generate power at non-powered dams offers a dual benefit; producing electricity, and acting as downstream fish passage, helping to reconnect previously separated ecosystems. In this study, we assess the technical, environmental, social, and economic feasibility of generating power at non-powered U.S. dam sites using Archimedes screw turbines by integrating mechanical constraints, social impact metrics, proximity to infrastructure, and environmental sensitivity data. Results account for future precipitation predictions and show, between 2024 and 2050, the number of sites where Archimedes screw turbines are viable decreases by one site, but overall generation capacity increases due to increased flow rates across persisting locations. Our analysis identified 82 non-powered dam sites with a mean generation capacity of 49 kW that meet the mechanical requirements for Archimedes screw turbine technology in 2024. Our analysis presents a framework for considering social, environmental, and economic impacts of specific turbine technologies to convert non-powered dams to generate power.

Archimedes screw turbine↗

2040 Vision Study: an Enlargement of Model Based Engineering

Over the last few decades, advances in high-performance computing, new materials characterization methods, and, more recently, an emphasis on integrated computational materials engineering (ICME) and additive manufacturing have been a catalyst for multiscale modeling and simulation-based design of materials and structures in the aerospace industry. As a result, NASA's Transformational Tools and Technology (TTT) Project sponsored a study (performed by a team led by Pratt & Whitney) to define the potential 25-year future state required for integrated multiscale modeling of materials and systems (e.g., load-bearing structures) to accelerate the pace and reduce the expense of innovation in future aerospace and aeronautical systems. This talk will briefly review the findings of this 2040 Vision study (e.g., the 2040 vision state; the required interdependent core technical work areas, Key Element (KE); associated critical gaps and actions to close those gaps; and major recommendations). The study, NASA CR 2018- 219771, envisions the development of a cyber-physical-social ecosystem comprised of experimentally verified and validated computational models, tools, and techniques, along with the associated digital tapestry, that marries two non-mutually exclusive paradigms _ "design of the materials" (material scientist viewpoint) and "design with the materials" (structural analyst viewpoint) _ into a concurrent transformational paradigm that impacts the entire supply chain to enable cost-effective, rapid, and revolutionary design of fit-for-purpose materials, components, and systems. Although the vision focused on aeronautics and space applications, it is believed that other engineering communities (e.g., automotive, biomedical, etc.) can benefit as well from the proposed framework with only minor modifications. Finally, it is TTT's hope and desire that this vision provides the strategic guidance to both public and private research and development decision makers to make the proposed 2040 vision state a reality and thereby provide a significant advancement in the United States global competitiveness.

Arnold, Steven M.↗

US Department of Energy, Office of Science High Performance Computing Facility Operational Assessment 2021: Oak Ridge Leadership Computing Facility

Oak Ridge National Laboratory’s (ORNL’s) Leadership Computing Facility (OLCF) continues to surpass its operational target goals of supporting users; delivering fast, reliable computational ecosystems; creating innovative solutions for high-performance computing (HPC) needs; contributing to the community to build the next generation HPC workforce, and managing risks, safety, and security associated with operating some of the most powerful computers in the world. The results can be seen in the cutting-edge science conducted by users and the praise from the research community. Calendar year (CY) 2021 saw continued excellence in research supported by the OLCF’s leadership-class computing resources, including Summit (the nation’s most powerful supercomputer), the global scratch file system Alpine, the Scalable Protected Infrastructure (SPI), the Exploratory Visualization Environment for Research in Science and Technology (EVEREST), and the archival mass-storage resource High-Performance Storage System (HPSS). While maintaining access and exceptional user support for Summit, the OLCF continued to make progress on the installation and deployment of Frontier, which will be the nation’s first exascale system when it comes online at the start of CY 2023. Users have already begun running and optimizing scientific codes on Crusher, the OLCF test and development system equipped with Frontier’s architecture. Throughout the year, the OLCF maintained a strong culture of operational excellence, including risk management, workplace safety, and cybersecurity. The OLCF’s rigorous risk management strategy anticipated and mitigated risks, and at this time there are no high-priority operational risks. Similarly, ORNL and the OLCF were committed to operating under the US Department of Energy’s (DOE’s) safety regulations that ensure a safe workplace. Technical staff tracked and monitored existing threats and vulnerabilities within the OLCF while continually developing tools and practices to enhance operations without increasing the facility’s risk. CY 2021 was filled with outstanding results and accomplishments, including a very high rating from users on overall satisfaction for the eighth consecutive year; a tremendous number of node hours delivered to 1,671 researchers on Summit; and the successful delivery of the allocation split of roughly 60%, 20%, and 20% of core-hours offered for the Innovative and Novel Computational Impact on Theory and Experiment (INCITE), Advanced Scientific Computing Research Leadership Computing Challenge (ALCC), and Director’s Discretionary (DD) programs, respectively (Section 2). COVID-19 research remained a focus in 2021, and the ALCC and DD programs allocated over 1 million Summit hours to the COVID-19 High Performance Computing Consortium. These accomplishments, coupled with the high utilization rates (i.e., overall and capability usage), represent the fulfillment of the promise of leadership class machines: efficient facilitation of leadership-class computational applications.

97 MATHEMATICS AND COMPUTING↗

Large-Scale CO2 Storage and Reservoir Management Strategies: Case Studies

This poster describes large-scale CO2 storage and reservoir management case studies for presentation at the AGU2024 conference. The analysis in this study provides a wealth of details on the designs and strategies that can lead to an optimal solution for pressure and CO2 plume management under critical constraints. These findings not only promise a reduction in the CO2 storage footprint but also hold the potential to create a more environmentally friendly ecosystem. Furthermore, this research offers valuable insights into the decision-making process when considering multi-project deployment in a shared basin for large-scale and gigatons of CO2 storage, paving the way for a more sustainable future. Poster presented (virtual) at the 2024 AGU Conference, December 9-13, 2024, Washington, D.C.

Liu, Guoxiang [NETL]↗

LANDSCAPE MANAGEMENT FOR SUSTAINABLE SUPPLIES OF BIOENERGY FEEDSTOCK AND ENHANCED SOIL QUALITY

Agriculture can simultaneously address global food, feed, fiber, and energy challenges provided our soil, water, and air resources are not compromised in doing so. As we embark on the 19th Triennial Conference of the International Soil and Tillage Research Organization (ISTRO), I am pleased to proclaim that our members are well poised to lead these endeavors because of our comprehensive understanding of soil, water, agricultural and bio-systems engineering processes. The concept of landscape management, as an approach for integrating multiple bioenergy feedstock sources, including biomass residuals, into current crop production systems, is used as the focal point to show how these ever-increasing global challenges can be met in a sustainable manner. Starting with the 2005 Billion Ton Study (BTS) goals, research and technology transfer activities leading to the 2011 U.S. Department of Energy (DOE) Revised Billion Ton Study (BT2) and development of a residue management tool to guide sustainable crop residue harvest will be reviewed. Multi-location USDA-Agricultural Research Service (ARS) Renewable Energy Assessment Project (REAP) team research and on-going partnerships between public and private sector groups will be shared to show the development of landscape management strategies that can simultaneously address the multiple factors that must be balanced to meet the global challenges. Effective landscape management strategies recognize the importance of nature’s diversity and strive to emulate those conditions to sustain multiple critical ecosystem services. To illustrate those services, the soil quality impact of harvesting crop residues are presented to show how careful, comprehensive monitoring of soil, water and air resources must be an integral part of sustainable bioenergy feedstock production systems. Preliminary analyses suggest that to sustain soil resources within the U.S. Corn Belt, corn (Zea mays L.) stover should not be harvested if average grain yields are less than 11 Mg ha-1 (175 bu ac-1) unless more intensive landscape management practices are implemented. Furthermore, although non-irrigated corn grain yields east and west of the primary Corn Belt may not consistently achieve the 11 Mg ha-1 yield levels, corn can still be part of an overall landscape approach for sustainable feedstock production. Another option for producers with consistently high yields (> 12.6 Mg ha-1 or 200 bu ac-1) that may enable them to sustainably harvest even more stover is to decrease their tillage intensity which will reduce fuel use, preserve rhizosphere carbon, and/or help maintain soil structure and soil quality benefits often attributed to no-till production systems. In conclusion, I challenge all ISTRO scientists to critically ask if your research is contributing to improved soil and crop management strategies that effectively address the complexity associated with sustainable food, feed, fiber and fuel production throughout the world.

09 BIOMASS FUELS↗

Understanding and regulation of microbial lignolysis for renewable platform chemicals

Lignin is often overlooked in the valorization of lignocellulosic biomass, but lignin-based materials and chemicals represent potential value-added products for biorefineries that could significantly improve the economics of a biorefinery. Fluctuating crude oil prices and changing fuel specifications are some of the driving factors to develop new technologies that could be used to convert polymeric lignin into low molecular weight lignin and or monomeric aromatic feedstocks to assist in the displacement of the current products associated with the conversion of a whole barrel of oil. Our project of understanding microbial lignolysis for renewable platform chemicals aimed to understand microbial and enzymatic lignolysis processes to break down lignin for conversion into commercially viable drop-in fuels. We developed novel lignin analytics to interrogate enzymatic and microbial lignolysis of native polymeric lignin and established a detailed understanding of lignolysis as a function of fungal enzyme, microbes and endophytes. Bioinformatics pipeline was developed for metatranscryptomic analysis of aridland ecosystem for investigating the potential discovery of new lignolysis gene and gene products.

09 BIOMASS FUELS↗

Institute for Sustained Performance, Energy, and Resilience (SuPER)

The University of Tennessee (UTK) and University of Texas at El Paso (UTEP) partnership supported the three main thrusts of the SUPER project---performance, energy, and resilience. The UTK-UTEP effort thus helped advance the main goal of SUPER, which was to ensure that DOE's computational scientists can successfully exploit the emerging generation of high performance computing (HPC) systems. This goal is being met by providing application scientists with strategies and tools to productively maximize performance, conserve energy, and attain resilience. The primary vehicle through which UTK provided performance measurement support to SUPER and the larger HPC community is the Performance Application Programming Interface (PAPI). PAPI is an ongoing project that provides a consistent interface and methodology for collecting hardware performance information from various hardware and software components, including most major CPUs, GPUs and accelerators, interconnects, I/O systems, and power interfaces, as well as virtual cloud environments. The PAPI software is widely used for performance modeling of scientific and engineering applications---for example, the HOMME (High Order Methods Modeling Environment) climate code, and the GAMESS and NWChem computational chemistry codes---on DOE supercomputers. PAPI is widely deployed as middleware for use by higher-level profiling, tracing, and sampling tools (e.g., CrayPat, HPCToolkit, Scalasca, Score-P, TAU, Vampir, PerfExpert), making it the de facto standard for hardware counter analysis. PAPI has established itself as fundamental software infrastructure in every application domain (spanning academia, government, and industry), where improving performance can be mission critical. Ultimately, as more application scientists migrate their applications to HPC platforms, they will benefit from the extended capabilities this grant brought to PAPI to analyze and optimize performance in these environments, whether they use PAPI directly, or via third-party performance tools. Capabilities added to PAPI through this grant include support for new architectures such as the lastest GPU and Xeon Phi accelerators, and advanced power measurement and management features. Another important topic for the UTK team was providing support for a rich ecosystem of different fault management strategies in the context of parallel computing. Our long term efforts have been oriented toward proposing flexible strategies and providing building boxes that application developers can use to build the most efficient fault management technique for their application. These efforts span across the entire software spectrum, from theoretical models of existing strategies to easily assess their performance, to algorithmic modifications to take advantage of specific mathematical properties for data redundancy and to extensions to widely used programming paradigms to empower the application developers to deal with all types of faults. We have also continued our tight collaborations with users to help them adopt these technologies to ensure their application always deliver meaningful scientific data. Large supercomputer systems are becoming more and more power and energy constrained, and future systems and applications running on them will need to be optimized to run under power caps and/or minimize energy consumption. The UTEP team contributed to the SUPER energy thrust by developing power modeling methodologies and investigating power management strategies. Scalability modeling results showed that some applications can scale better with respect to an increasing power budget than with respect to only the number of processors. Power management, in particular shifting power to processors on the critical path of an application execution, can reduce perturbation due to system noise and other sources of runtime variability, which are growing problems on large-scale power-constrained computer systems.

42 ENGINEERING↗

Habitat Inspection Scanner, Bio-Structure Scanner, and In Situ Sub-Surface Composition Sensor

The extension of dielectric and inductive spectroscopy into in situ observations represents a significant exploration-enabling tool. This technology can be widely applied from microscopic to macroscopic. Dielectrometry and inductometry can measure sub-surface composition and its distribution. The primary environment that we cannot easily explored is the sub-surface of solid bodies. Weather as part of our equipment that we bring with us, or the locations we are exploring. These fundamental questions lie at the core of the exploration Initiative. To answer them we must use a whole host of complimentary tools including those that allow us to practically examine the sub-surface environment. A nondestructive approach offers significant advantages for both the initial identification of likely samples but also the monitoring of ecosystems and crew health. These include materials characterization, nondestructive inspection, and process quality control, damage monitoring, and hidden object detection and identification. The identification of natural resources such as water on the Moon or Mars is of great importance to the utilization of local resource in the support of human exploration crews. On the macroscopic scale, the understanding of what resources are available and how they are distributed is of primary importance to their productive utilization. Even if initial explorations do not require the use of local resources to succeed, eventual settlement and commercial development will. The routine examination of the structural integrity (micro cracks, leaks) of hi.inafi habitats in harsh envkmments ww!d also be enabled.

VanSteenberg, Michael↗

Sim2Ls: FAIR simulation workflows and data

Just like the scientific data they generate, simulation workflows for research should be findable, accessible, interoperable, and reusable (FAIR). However, while significant progress has been made towards FAIR data, the majority of science and engineering workflows used in research remain poorly documented and often unavailable, involving ad hoc scripts and manual steps, hindering reproducibility and stifling progress. We introduce Sim2Ls (pronounced simtools) and the Sim2L Python library that allow developers to create and share end-to-end computational workflows with well-defined and verified inputs and outputs. The Sim2L library makes Sim2Ls , their requirements, and their services discoverable, verifies inputs and outputs, and automatically stores results in a globally-accessible simulation cache and results database. This simulation ecosystem is available in nanoHUB, an open platform that also provides publication services for Sim2Ls , a computational environment for developers and users, and the hardware to execute runs and store results at no cost. We exemplify the use of Sim2Ls using two applications and discuss best practices towards FAIR simulation workflows and associated data.

59 BASIC BIOLOGICAL SCIENCES↗

Developing closed life support systems for large space habitats

In anticipation of possible large-scale, long-duration space missions which may be conducted in the future, NASA has begun to investigate the research and technology development requirements to create life support systems for large space habitats. An analysis suggests the feasibility of a regeneration of food in missions which exceed four years duration. Regeneration of food in space may be justified for missions of shorter duration when large crews must be supported at remote sites such as lunar bases and space manufacturing facilities. It is thought that biological components consisting principally of traditional crop and livestock species will prove to be the most acceptable means of closing the food cycle. A description is presented of the preliminary results of a study of potential biological components for large space habitats. Attention is given to controlled ecosystems, Russian life support system research, controlled-environment agriculture, and the social aspects of the life-support system.

Phillips, J. M.↗

Trade-offs among restored ecosystem functions are context-dependent in Mediterranean-type regions

Global biodiversity hotspots, including Mediterranean-type ecosystems worldwide, are highly threatened by global change that alters biodiversity, ecosystem functions, and services. Some restoration activities enhance ecosystem functions by reintroducing plant species based on known relationships between plant traits and ecosystem processes. Achieving multiple functions across different site conditions, however, requires understanding how abiotic factors like climate and soil, along with plant assemblages, influence ecosystem functions, including their trade-offs and synergies. We used the ModEST ecosystem simulation model, which integrates carbon, water, and nutrient processes with plant traits, to assess the relationships between restored plant assemblages and ecosystem functions in Mediterranean-type climates and soils. We investigated whether maximised carbon increment, water use efficiency, and nitrogen use efficiency, along with their trade-offs and synergies, varied across different abiotic contexts. Further, we asked whether assemblages that maximised functions varied across environments and among these functions. We found that maximised ecosystem carbon increment and nitrogen use efficiency occurred under moist, warm conditions, while water use efficiency peaked under drier conditions. Generally, the assemblage that maximised one function differed from those for other maximised functions. Synergies were rare, except between water and nitrogen use efficiencies in loam soils across most climates. Trade-offs among maximised functions were common, varying in strength with abiotic context and plant assemblages, and were more pronounced in sandy loam soils compared to clay-rich soils. Our findings suggest that due to variation in abiotic conditions within and across Mediterranean-type regions at the global scale, site-specific plant assemblages are required to maximise ecosystem functions. Thus, lessons from a single site cannot be transferred to another site, even where the same plant functional types are available for restoration. Our simulation results offer valuable insights into potential ecosystem performance under specific abiotic conditions following restoration with particular plant functional types, thereby informing local restoration efforts.

54 ENVIRONMENTAL SCIENCES↗

Priming Effects in Boreal Black Spruce Forest Soils: Quantitative Evaluation and Sensitivity Analysis

Laboratory studies show that introduction of fresh and easily decomposable organic carbon (OC) into soil-water systems can stimulate the decomposition of soil OC (SOC) via priming effects in temperate forests, shrublands, grasslands, and agroecosystems. However, priming effects are still not well understood in the field setting for temperate ecosystems and virtually nothing is known about priming effects (e.g., existence, frequency, and magnitude) in boreal ecosystems. In this study, a coupled dissolved OC (DOC) transport and microbial biomass dynamics model was developed to simultaneously simulate co-occurring hydrological, physical, and biological processes and their interactions in soil pore-water systems. The developed model was then used to examine the importance of priming effects in two black spruce forest soils, with and without underlying permafrost. Our simulations showed that priming effects were strongly controlled by the frequency and intensity of DOC input, with greater priming effects associated with greater DOC inputs. Sensitivity analyses indicated that priming effects were most sensitive to variations in the quality of SOC, followed by variations in microbial biomass dynamics (i.e., microbial death and maintenance respiration), highlighting the urgent need to better discern these key parameters in future experiments and to consider these dynamics in existing ecosystem models. Water movement carries DOC to deep soil layers that have high SOC stocks in boreal soils. Thus, greater priming effects were predicted for the site with favorable water movement than for the site with limited water flow, suggesting that priming effects might be accelerated for sites where permafrost degradation leads to the formation of dry thermokarst.

54 ENVIRONMENTAL SCIENCES↗

Portable nitrous oxide sensor for understanding agricultural and soil emissions

Nitrous oxide (N2O) is the third most important greenhouse gas (GHG,) with an atmospheric lifetime of ~114 years and a global warming impact ~300 times greater than that of carbon dioxide. The main cause of nitrous oxide’s atmospheric increase is anthropogenic emissions, and over 80% of the current global anthropogenic flux is related to agriculture, including associated land-use change. An accurate assessment of N2O emissions from agriculture is vital not only for understanding the global N2O balance and its impact on climate but also for designing crop systems with lower GHG emissions. Such assessments are currently hampered by the lack of instrumentation and methodologies to measure ecosystem-level fluxes at appropriate spatial and temporal scales. Southwest Sciences and Princeton University are developing and testing new open-path eddy covariance instrumentation for continuous and fast (10 Hz) measurement of nitrous oxide emissions. An important advance, now being implemented, is the use of new mid-infrared laser sources that enable the development of exceptionally low power (<10 W) compact instrumentation that can be used even in remote sites lacking in power. The instrumentation will transform the ability to measure and understand ecosystem-level nitrous oxide fluxes. The Phase II results included successful extended field testing of prototype flux instruments, based on quantum cascade lasers, in collaboration with Michigan State University. Results of these tests demonstrated a flux detection limit of 5 µg m-2 s-1 and showed excellent agreement and correlation with measurements using chamber techniques. Initial tests of an instrument using an interband cascade laser (ICL) were performed, verifying that an order of magnitude reduction in instrument power requirements can be realized. These results point toward future improvements and testing leading to introduction of a commercial open path instrument for N2O flux measurements that is truly portable and cost-effective. The technology developed on this project is especially groundbreaking as it could be widely applied across FLUXNET and AmeriFlux sites (>1200 worldwide) for direct measurements of N2O exchange. The technology can be more broadly applied to gas monitoring requirements in industry, environmental monitoring, health and safety, etc.

47 OTHER INSTRUMENTATION↗

Plant Single-Cell Solutions for Energy and the Environment (Second Workshop Report)

Plants are important sources of energy and materials, and they collectively represent a critical component of Earth’s ecosystem. With increasing environmental stresses due to climate change and intensive agricultural practices, the need for resilient plants is greater than ever before. To secure plant resources for bioenergy, biomaterials, food, and ecosystem adaptation, a deeper understanding of the fundamental biology of plants at a cellular level is urgently needed. Plants contain a multitude of specialized cell types that compose tissues and organs. Pathogens often target specific cell types within plants, and the response of one cell to a particular stimulus is likely to be distinct from its neighbor because of underlying molecular and contextual differences. Understanding how these responses are distributed among cells, the main goal of single-cell approaches, will substantially enhance our ability to use targeted engineering for improving plant productivity and resilience. Furthermore, single-cell approaches are necessary to understand the interactions between plants and other ecosystem members such as fungi, bacteria, and archaea. Unlocking these gene-response mechanisms at a cellular level can improve our ability to adapt plants to environmental stresses, increasing their utility as feedstocks for biomaterials and bioenergy. Recent advances in high-throughput sequencing, mass spectrometry, microfluidics and miniaturization, artificial intelligence and machine learning, and bioinformatics have greatly improved our ability to detect and understand processes at a cellular level. In mammalian systems, single-cell transcriptomics has already led to many advances, such as newly identified cell types and cell-targeted treatment of diseases, and mass spectrometry-based single-cell proteomics has recently been demonstrated as a promising emerging technology. However, plant single-cell omics has lagged behind mammalian approaches due to the high cost of the technologies relative to available resources and to the innate biological features of plants, including the complexity of the cell wall and polyploidy. To better understand how single-cell methods could enable plant science, Lawrence Berkeley National Laboratory (Berkeley Lab) hosted a workshop on April 29, 2021, that brought together a diverse group of leaders in plant and/or single-cell biology. Attendees represented federal research programs and domestic and international academic institutions. During the workshop, three presenters described the current state of research in both experimental and computational approaches. While the focus of the workshop was on factors preventing plant biology researchers from fully adopting single-cell methodologies, workshop participants agreed that most barriers could be overcome with focused, strategic investment and coordinated efforts among institutions leading to significant scientific discoveries that would be difficult to obtain using more conventional technologies.

59 BASIC BIOLOGICAL SCIENCES↗