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At least 811 records · Page 45

A computer aided thermodynamic approach for predicting the formation of Z-DNA in naturally occurring sequences

The ease with which a particular DNA segment adopts the left-handed Z-conformation depends largely on the sequence and on the degree of negative supercoiling to which it is subjected. We describe a computer program (Z-hunt) that is designed to search long sequences of naturally occurring DNA and retrieve those nucleotide combinations of up to 24 bp in length which show a strong propensity for Z-DNA formation. Incorporated into Z-hunt is a statistical mechanical model based on empirically determined energetic parameters for the B to Z transition accumulated to date. The Z-forming potential of a sequence is assessed by ranking its behavior as a function of negative superhelicity relative to the behavior of similar sized randomly generated nucleotide sequences assembled from over 80,000 combinations. The program makes it possible to compare directly the Z-forming potential of sequences with different base compositions and different sequence lengths. Using Z-hunt, we have analyzed the DNA sequences of the bacteriophage phi X174, plasmid pBR322, the animal virus SV40 and the replicative form of the eukaryotic adenovirus-2. The results are compared with those previously obtained by others from experiments designed to locate Z-DNA forming regions in these sequences using probes which show specificity for the left-handed DNA conformation.

Non-NASA Center↗

Effects of Gravity on ZBLAN Glass Crystallization

The effects of gravity on the crystallization of ZrF(4)-BaF(2)-LaF(3)-AIF(3)-NaF glasses have been studied using the NASA KC-135 and a sounding rocket. Fibers and cylinders of ZBLAN glass were heated to the crystallization temperature in unit and reduced gravity. When processed in unit gravity the glass crystallized, but when processed in reduced gravity, crystallization was suppressed. A possible explanation involving shear thinning is presented to explain these results.

Gravitation↗

Flow field topology of transient mixing driven by buoyancy

Transient mixing driven by buoyancy occurs through the birth of a symmetric Rayleigh-Taylor morphology (RTM) structure for large length scales. Beyond its critical bifurcation the RTM structure exhibits self-similarity and occurs on smaller and smaller length scales. The dynamics of the RTM structure, its nonlinear growth and internal collision, show that its genesis occurs from an explosive bifurcation which leads to the overlap of resonance regions in phase space. This event shows the coexistence of regular and chaotic regions in phase space which is corroborated with the existence of horseshoe maps. A measure of local chaos given by the topological entropy indicates that as the system evolves there is growth of uncertainty. Breakdown of the dissipative RTM structure occurs during the transition from explosive to catastrophic bifurcation; this event gives rise to annihilation of the separatrices which drives overlap of resonance regions. The global bifurcation of explosive and catastrophic events in phase space for the large length scale of the RTM structure serves as a template for which mixing occurs on smaller and smaller length scales. Copyright 2004 American Institute of Physics.

Nonlinear Dynamics↗

Analysis of ETMS Data Quality for Traffic Flow Management Decisions

The data needed for air traffic flow management decision support tools is provided by the Enhanced Traffic Management System (ETMS). This includes both the tools that are in current use and the ones being developed for future deployment. Since the quality of decision support provided by all these tools will be influenced by the quality of the input ETMS data, an assessment of ETMS data quality is needed. Motivated by this desire, ETMS data quality is examined in this paper in terms of the unavailability of flight plans, deviation from the filed flight plans, departure delays, altitude errors and track data drops. Although many of these data quality issues are not new, little is known about their extent. A goal of this paper is to document the magnitude of data quality issues supported by numerical analysis of ETMS data. Guided by this goal, ETMS data for a 24-hour period were processed to determine the number of aircraft with missing flight plan messages at any given instant of time. Results are presented for aircraft above 18,000 feet altitude and also at all altitudes. Since deviation from filed flight plan is also a major cause of trajectory-modeling errors, statistics of deviations are presented. Errors in proposed departure times and ETMS-generated vertical profiles are also shown. A method for conditioning the vertical profiles for improving demand prediction accuracy is described. Graphs of actual sector counts obtained using these vertical profiles are compared with those obtained using the Host data for sectors in the Fort Worth Center to demonstrate the benefit of preprocessing. Finally, results are presented to quantify the extent of data drops. A method for propagating track positions during ETMS data drops is also described.

Chatterji, Gano B.↗

High-resolution spectroscopy of H2SO4, HDSO4, and D2SO4 vapor in the region 1200-10,000 cm(-1)

The high-resolution (0.05 cm(-1)) spectra of gas-phase H2SO4, HDSO4, and D2SO4 were measured over the frequency region 1200-10,000 cm(-1) using Fourier-transform infrared spectroscopy. The increased resolution of this work compared with previous studies has lead to an improved vibrational analysis of H2SO4. This study has answered unresolved questions about combination bands and overlapping features from previous gas-phase spectroscopic studies of H2SO4 and marks the first experimental measurement of the nu8 and nu15 torsional vibrations in this molecule. This work leads to a brief discussion on vibrational mode mixing in sulfuric acid.

Deuterium/chemistry↗

The Strings of Eta Carina: The HST/STIS Spectra and [Ca II]

Long linear, filamentary ejecta, are found to move at very high velocity external to the Homunculus, the circumstellar hourglass-shaped ejecta surrounding Eta Carinae. The origin of the strings is a puzzle. As an example, the Weigelt Blobs have N at 10X solar and C, O at 0.01X solar abundance, along with He/H significantly enhanced. This abundance pattern is evidence for extreme CNO-processing. Similarly, the Strontium Filament has Ti/Ni at 100X solar, presumably due to the lack of oxygen to form Ti-oxide precipitates onto dust grains. We have obtained 2-D spectra with the HST/STIS of the Strontium Filament and a portion of a string. These deep spectral exposures, at moderate dispersion, span much of the near red spectral region from 5000 to 9000A. We have identified twelve emission lines in these spectra with proper velocities and spatial structure of this string and obtained line ratios for [Ca II] (7293/7325A) and [Fe Ill (7157/8619A) which are useful for determining physical conditions in this nebulosity. In an attempt to use the [Ca II] ratio to determine the physical parameters, and ultimately the abundances in the strings, we have constructed a statistical equilibrium model for Ca II , including radiative and collisional rates. These results incorporate our newly calculated atomic data for levels n = 3,4,5 and 6 configurations of Ca II. The aim is to compute the [Ca II] line ratios and use them as a diagnostic of the physical parameters. Using the [Fe II] ratio we find that for Te=10,000 K, the electron density is Ne approx.10(exp 6)/cu cm. We plan to use the [Ca II] ratio to confirm this result. Then, we will extend the use of this multilevel model Ca II atom to study the physical conditions of the Strontium filament where eight lines of Ca II, both allowed and forbidden, had been identified. With the physical conditions determined, we will be able to derive reliable estimates for the gas phase abundances in the strings.

Melendez, M. B.↗

A Framework for Performing Multiscale Stochastic Progressive Failure Analysis of Composite Structures

A framework is presented that enables coupled multiscale analysis of composite structures. The recently developed, free, Finite Element Analysis - Micromechanics Analysis Code (FEAMAC) software couples the Micromechanics Analysis Code with Generalized Method of Cells (MAC/GMC) with ABAQUS to perform micromechanics based FEA such that the nonlinear composite material response at each integration point is modeled at each increment by MAC/GMC. As a result, the stochastic nature of fiber breakage in composites can be simulated through incorporation of an appropriate damage and failure model that operates within MAC/GMC on the level of the fiber. Results are presented for the progressive failure analysis of a titanium matrix composite tensile specimen that illustrate the power and utility of the framework and address the techniques needed to model the statistical nature of the problem properly. In particular, it is shown that incorporating fiber strength randomness on multiple scales improves the quality of the simulation by enabling failure at locations other than those associated with structural level stress risers.

Bednarcyk, Brett A.↗

A Framework for Performing Multiscale Stochastic Progressive Failure Analysis of Composite Structures

A framework is presented that enables coupled multiscale analysis of composite structures. The recently developed, free, Finite Element Analysis-Micromechanics Analysis Code (FEAMAC) software couples the Micromechanics Analysis Code with Generalized Method of Cells (MAC/GMC) with ABAQUS to perform micromechanics based FEA such that the nonlinear composite material response at each integration point is modeled at each increment by MAC/GMC. As a result, the stochastic nature of fiber breakage in composites can be simulated through incorporation of an appropriate damage and failure model that operates within MAC/GMC on the level of the fiber. Results are presented for the progressive failure analysis of a titanium matrix composite tensile specimen that illustrate the power and utility of the framework and address the techniques needed to model the statistical nature of the problem properly. In particular, it is shown that incorporating fiber strength randomness on multiple scales improves the quality of the simulation by enabling failure at locations other than those associated with structural level stress risers.

Bednarcyk, Brett A.↗

Prebreathe Protocol for Extravehicular Activity Technical Consultation Report

In the performance of EVA by that National Aeronautics and Space Administration (NASA) astronauts, there exists a risk of DCS as the suit pressure is reduced to 4.3 pounds per square inch, absolute (psia) from the International Space Station (ISS) pressure of 14.7 psia. Several DCS-preventive procedures have been developed and implemented. Each of these procedures involve the use of oxygen (O2) prebreathe to effectively washout tissue nitrogen (N2).The management of the ISS Programs convened an expert independent peer review Team to conduct a review of the Decompression Sickness (DCS) risks associated with the Extra Vehicular Activity (EVA) Campout Prebreathe (PB) protocol for its consideration for use on future missions. The major findings and recommendations of the expert panel are: There is no direct experimental data to confirm the potential DCS risks of the Campout PB protocol. However, based on model data, statistical probability, physiology, and information derived from similar PB protocols, there is no compelling evidence to suggest that the Campout PB protocol is less safe than the other NASA approved PB protocols.

Ross, Jerry↗

The Effect of Aerosols and Clouds on the Retrieval of Infrared Sea Surface Temperatures

Comparisons are performed between spatially averaged sea surface temperatures (ASST2) as derived from the second Along-Track Scanning Radiometer (ATSR-2) on board the second European Remote Sensing Satellite (ERS-2) and the NOAA-NASA Advanced Very High Resolution Radiometer (AVHRR) Oceans Pathfinder dataset (MPFSST). Difference maps, MPFSST 2 ASST2, along with the application of a simple statistical regression model to aerosol and cloud data from the Total Ozone Mapping Spectrometer ( TOMS), are used to examine the impact of possible aerosol and cloud contamination. Differences varied regionally, but the largest biases were seen off western Africa. Nighttime and daytime differences off western Africa were reduced from -0.5degrees to -0.2degreesC and from -0.1degrees to 0degreesC, respectively. Significant cloud flagging, based on the model, occurred in the Indian Ocean, the equatorial Pacific, and in the vicinity of the Gulf Stream. Comparisons of the MPFSST and the ASST2 with in situ data from the 2002 version of the World Oceanic Database (WOD02) off western Africa show larger mean differences for the MPFSST. The smallest mean differences occurred for nighttime ASST2 - WOD02 with a value of 0.0degrees +/- 0.4degreesC.

clouds↗

Synthesis and Assimilation Systems - Essential Adjuncts to the Global Ocean Observing System

Ocean assimilation systems synthesize diverse in situ and satellite data streams into four-dimensional state estimates by combining the various observations with the model. Assimilation is particularly important for the ocean where subsurface observations, even today, are sparse and intermittent compared with the scales needed to represent ocean variability and where satellites only sense the surface. Developments in assimilation and in the observing system have advanced our understanding and prediction of ocean variations at mesoscale and climate scales. Use of these systems for assessing the observing system helps identify the strengths of each observation type. Results indicate that the ocean remains under-sampled and that further improvements in the observing system are needed. Prospects for future advances lie in improved models and better estimates of error statistics for both models and observations. Future developments will be increasingly towards consistent analyses across components of the Earth system. However, even today ocean synthesis and assimilation systems are providing products that are useful for many applications and should be considered an integral part of the global ocean observing and information system.

Rienecker, Michele M.↗

Prediction of Muscle Performance During Dynamic Repetitive Exercise

A method for predicting human muscle performance was developed. Eight test subjects performed a repetitive dynamic exercise to failure using a Lordex spinal machine. Electromyography (EMG) data was collected from the erector spinae. Evaluation of the EMG data using a 5th order Autoregressive (AR) model and statistical regression analysis revealed that an AR parameter, the mean average magnitude of AR poles, can predict performance to failure as early as the second repetition of the exercise. Potential applications to the space program include evaluating on-orbit countermeasure effectiveness, maximizing post-flight recovery, and future real-time monitoring capability during Extravehicular Activity.

Byerly, D. L.↗

Prediction of Seasonal Climate-induced Variations in Global Food Production

Consumers, including the poor in many countries, are increasingly dependent on food imports and are therefore exposed to variations in yields, production, and export prices in the major food-producing regions of the world. National governments and commercial entities are paying increased attention to the cropping forecasts of major food-exporting countries as well as to their own domestic food production. Given the increased volatility of food markets and the rising incidence of climatic extremes affecting food production, food price spikes may increase in prevalence in future years. Here we present a global assessment of the reliability of crop failure hindcasts for major crops at two lead times derived by linking ensemble seasonal climatic forecasts with statistical crop models. We assessed the reliability of hindcasts (i.e., retrospective forecasts for the past) of crop yield loss relative to the previous year for two lead times. Pre-season yield predictions employ climatic forecasts and have lead times of approximately 3 to 5 months for providing information regarding variations in yields for the coming cropping season. Within-season yield predictions use climatic forecasts with lead times of 1 to 3 months. Pre-season predictions can be of value to national governments and commercial concerns, complemented by subsequent updates from within-season predictions. The latter incorporate information on the most recent climatic data for the upcoming period of reproductive growth. In addition to such predictions, hindcasts using observations from satellites were performed to demonstrate the upper limit of the reliability of crop forecasting.

variations↗

Improved Broadband Liner Optimization Applied to the Advanced Noise Control Fan

The broadband component of fan noise has grown in relevance with the utilization of increased bypass ratio and advanced fan designs. Thus, while the attenuation of fan tones remains paramount, the ability to simultaneously reduce broadband fan noise levels has become more desirable. This paper describes improvements to a previously established broadband acoustic liner optimization process using the Advanced Noise Control Fan rig as a demonstrator. Specifically, in-duct attenuation predictions with a statistical source model are used to obtain optimum impedance spectra over the conditions of interest. The predicted optimum impedance information is then used with acoustic liner modeling tools to design liners aimed at producing impedance spectra that most closely match the predicted optimum values. Design selection is based on an acceptance criterion that provides the ability to apply increased weighting to specific frequencies and/or operating conditions. Constant-depth, double-degree of freedom and variable-depth, multi-degree of freedom designs are carried through design, fabrication, and testing to validate the efficacy of the design process. Results illustrate the value of the design process in concurrently evaluating the relative costs/benefits of these liner designs. This study also provides an application for demonstrating the integrated use of duct acoustic propagation/radiation and liner modeling tools in the design and evaluation of novel broadband liner concepts for complex engine configurations.

Nark, Douglas M.↗

Method for Real-Time Model Based Structural Anomaly Detection

A system and methods for real-time model based vehicle structural anomaly detection are disclosed. A real-time measurement corresponding to a location on a vehicle structure during an operation of the vehicle is received, and the real-time measurement is compared to expected operation data for the location to provide a modeling error signal. A statistical significance of the modeling error signal to provide an error significance is calculated, and a persistence of the error significance is determined. A structural anomaly is indicated, if the persistence exceeds a persistence threshold value.

Smith, Timothy A.↗

Soil Dust Aerosols and Wind as Predictors of Seasonal Meningitis Incidence in Niger

Background: Epidemics of meningococcal meningitis are concentrated in sub-Saharan Africa during the dry season, a period when the region is affected by the Harmattan, a dry and dusty northeasterly trade wind blowing from the Sahara into the Gulf of Guinea.Objectives: We examined the potential of climate-based statistical forecasting models to predict seasonal incidence of meningitis in Niger at both the national and district levels.Data and methods: We used time series of meningitis incidence from 1986 through 2006 for 38 districts in Niger. We tested models based on data that would be readily available in an operational framework, such as climate and dust, population, and the incidence of early cases before the onset of the meningitis season in January-May. Incidence was used as a proxy for immunological state.

Soil dust↗

Meningitis and Climate: From Science to Practice

Meningococcal meningitis is a climate sensitive infectious disease. The regional extent of the Meningitis Belt in Africa, where the majority of epidemics occur, was originally defined by Lapeysonnie in the 1960s. A combination of climatic and environmental conditions and biological and social factors have been associated to the spatial and temporal patterns of epidemics observed since the disease first emerged in West Africa over a century ago. However, there is still a lack of knowledge and data that would allow disentangling the relative effects of the diverse risk factors upon epidemics. The Meningitis Environmental Risk Information Technologies Initiative (MERIT), a collaborative research-to-practice consortium, seeks to inform national and regional prevention and control strategies across the African Meningitis Belt through the provision of new data and tools that better determine risk factors. In particular MERIT seeks to consolidate a body of knowledge that provides evidence of the contribution of climatic and environmental factors to seasonal and year-to-year variations in meningococcal meningitis incidence at both district and national scales. Here we review recent research and practice seeking to provide useful information for the epidemic response strategy of National Ministries of Health in the Meningitis Belt of Africa. In particular the research and derived tools described in this paper have focused at "getting science into policy and practice" by engaging with practitioner communities under the umbrella of MERIT to ensure the relevance of their work to operational decision-making. We limit our focus to that of reactive vaccination for meningococcal meningitis. Important but external to our discussion is the development and implementation of the new conjugate vaccine, which specifically targets meningococcus A

Mineral dust↗