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At least 811 records · Page 45

Metadata Entry Optimization for NASA's Biological Institutional Scientific Collection (NBISC)

The NASA Biological Institutional Sample Collection (NBISC) at NASA’s Ames Research Center is a critical resource housing non-human samples collected from spaceflight missions and ground analog studies, primarily consisting of specimens from rats, mice, and select microbes. The primary objective of NBISC is to systematically receive, document, preserve, and facilitate access to these samples for the global scientific community. NBISC promotes international collaboration and maximizes the return on investment for precious tissues from spaceflight and analog experiments. Researchers can request physical samples through an online request form and subsequent written proposal review process. This study addresses two core research objectives: streamlining the NBISC sample lifecycle processes and strategizing for managing an influx of 50,000 tissue samples from a series of cosmic radiation analog experiments carried out at the NASA Space Radiation Laboratory (NSRL) by Drs. Eleanor Chang (Lawrence Berkeley Laboratory) and Polly Blakely (SRI). The Chang/Blakely studies investigated Harderian gland (HG) tumorigenesis in mice exposed to low dose and LET radiation comprising 8 different exposure protocols in over 4000 mice. NBISC sample metadata is stored in a Laboratory Information Management System (SLIMS). To streamline sample data entry, we customize python scripts using information extracted from the individual experimental protocols. The scripts automate entry into multiple SLIMS data fields including protocol name, unique sample barcode, tissue and sub-tissue information, freezer location, sample preservation method, etc. The semi-automated procedure significantly decreases the time spent on data entry by several orders of magnitude. Automation and data organization are essential, as they free up time for curation and promotion of the collection which, in turn, increase the accessibility of samples to the broader research community. NBISC benefits from streamlined data ingestion, and the methodologies developed here are applicable to other projects which use SLIMS including the NASA Biospecimen Sharing Program and GeneLab. As of Fall 2023, plans include transferring sample data from SLIMS to public facing repositories (OSDR and NLSP), expanding the reach of the Chang/Blakely sample collection. The Human Research Program Space Radiation Element plans to transfer non-human tissues from many more investigations to NBISC in the coming year.

Sample Repository↗

Metadata Entry Optimization For NASA's Biological Institutional Scientific Collection (NBISC)

The NASA Biological Institutional Sample Collection (NBISC) at NASA’s Ames Research Center is a critical resource housing non-human samples collected from spaceflight missions and ground analog studies, primarily consisting of specimens from rats, mice, and select microbes. The primary objective of NBISC is to systematically receive, document, preserve, and facilitate access to these samples for the global scientific community. NBISC promotes international collaboration and maximizes the return on investment for precious tissues from spaceflight and analog experiments. Researchers can request physical samples through an online request form and subsequent written proposal review process. This study addresses two core research objectives: streamlining the NBISC sample lifecycle processes and strategizing for managing an influx of 50,000 tissue samples from a series of cosmic radiation analog experiments carried out at the NASA Space Radiation Laboratory (NSRL) by Drs. Eleanor Chang (Lawrence Berkeley Laboratory) and Polly Blakely (SRI). The Chang/Blakely studies investigated Harderian gland (HG) tumorigenesis in mice exposed to low dose and LET radiation comprising 8 different exposure protocols in over 4000 mice. NBISC sample metadata is stored in a Laboratory Information Management System (SLIMS). To streamline sample data entry, we customize python scripts using information extracted from the individual experimental protocols. The scripts automate entry into multiple SLIMS data fields including protocol name, unique sample barcode, tissue and sub-tissue information, freezer location, sample preservation method, etc. The semi-automated procedure significantly decreases the time spent on data entry by several orders of magnitude. Automation and data organization are essential, as they free up time for curation and promotion of the collection which, in turn, increase the accessibility of samples to the broader research community. NBISC benefits from streamlined data ingestion, and the methodologies developed here are applicable to other projects which use SLIMS including the NASA Biospecimen Sharing Program and GeneLab. As of Fall 2023, plans include transferring sample data from SLIMS to public facing repositories (OSDR and NLSP), expanding the reach of the Chang/Blakely sample collection. The Human Research Program Space Radiation Element plans to transfer non-human tissues from many more investigations to NBISC in the coming year.

Biospecimen↗

Elevating the Quality of Space Omics Sequencing Data: Innovations and Methodologies from NASA GeneLab Sample Processing Laboratory

NASA’s GeneLab, part of the NASA Open Science Data Repository, is a space-related database that hosts a diverse range of transcriptomics, proteomics, epigenomics and genomics data. The NASA GeneLab Sample Processing Laboratory (SPL) generates omics data from biological experiments conducted aboard the International Space Station, Space Shuttle and space related ground experiments, this omics data then hosted on the GeneLab repository. Samples generated such experiments pose numerous technical challenges such as small experimental sample size, variance in dissection times, limited tissue preservation methods, prolonged storage time, and more. GeneLab SPL team had developed specialized expertise in nucleic acid extraction, library preparation and sequencing of such biological samples via extensive training and years of experience. In order to ensure data accuracy and consistency across experiments, SPL has developed standardized protocols for each species and tissue type. These protocols in conjunction with quality control metrics and data standards are crucial in generating of high-quality data. SPL protocols and standards have been developed in collaboration with the scientific community and had been made publicly available on the GeneLab portal, guaranteeing comparability of datasets across spaceflight experiments. To ensure reliability of data generation, SPL leverages cutting-edge innovations in laboratory automation for sample processing. By leveraging these state-of-the-art platforms, SPL achieves high levels of data reproducibility while significantly minimizing sources of bias and variability, especially across experiments with large numbers of samples. Over the past few years, the space biology investigator community has accessed SPL-generated data from the Open Science Data Repository for a myriad of data re-analysis and re-use studies. We observe a trend that in-house SPL-generated data consistently outperforms outsourced sequencing data in terms of technical standards, quality control metrics, timeliness of data delivery, and sequencing and reagent efficiency. Superior data generation has and will continue to enable discoveries in disease, diagnostic tools, and the biological effects of long duration spaceflight.

GeneLab↗

Extraterrestrial Molecular Indicators of Life Investigation (EMILI)

Future missions to Enceladus, Europa, Mars, and beyond may seek the molecular signs of extraterrestrial life through chemical analysis of acquired samples. Particularly on ocean worlds such as Enceladus and Europa, samples may contain trace ocean-borne molecular biosignatures of extant life that may or may not share similarities to those of terrestrial life. In situ analyses must be prepared to detect and characterize a wide range of possible molecular species, structures, and patterns, typically with exquisite sensitivity and within a complex, poorly-characterized planetary environment. The Extraterrestrial Molecular Indicators of Life Investigation (EMILI) is designed to meet or exceed the requirements of such missions for organic molecular analysis through a powerful combination of dual chemical separation and both optical and mass spectrometry detection techniques, realized in an integrated, compact instrument package fully compatible with anticipated flight resources and conditions. The full EMILI instrument combines two sample analysis subsystems to provide wide-ranging and complementary detection of organic compounds and inorganic salts. The Gas Analysis Processing System (GAPS) uses a chemical derivatization protocol with gas chromatography (GC) separation prior to detection in an ion trap mass spectrometer (ITMS) to enable full characterization of lower-polarity, volatile and semi-volatile molecules such as fatty acids and hydrocarbons. The Organic Capillary Electrophoresis ANalysis System (OCEANS) uses a liquid-based extraction protocol with CE separation to enable precise analysis of more water-soluble/polar compounds. OCEANS features a laser-induced fluorescence detection mode to perform ultra-sensitive quantitative analysis of chiral amino acids. In EMILI, OCEANS is additionally coupled to the same ITMS through a novel electrospray ionization interface. The common ITMS allows EMILI to identify and cross-correlate molecular species and patterns, detected through either or both protocols, of molecular weights to over 1000 u, potentially even revealing complex biosignatures such as alien oligopeptides and informational polymers.

Europa↗

Clock Synchronization Characterization of the Washington DC Metropolitan Area Quantum Network (DC-QNet)

Quantum networking protocols relying on interference and precise time-of-flight measurements require high-precision clock synchronization. This study describes the design, implementation, and characterization of two optical time transfer methods in a metropolitan-scale quantum networking research testbed. With active electronic stabilization, sub-picosecond time deviation (TDEV) was achieved at integration times between 1 s and 10 5 s over 53 km of deployed fiber. Over the same integration periods, 10-picosecond level TDEV was observed using the White Rabbit-Precision Time Protocol (WR-PTP) over 128 km. Measurement methods are described to understand the sources of environmental fluctuations on clock synchronization towards the development of in-situ compensation methods. Path delay gradients, chromatic dispersion, polarization drift, and optical power variations all contributed to clock synchronization errors. The results from this study will inform future work in the development of link noise management methods essential for enabling experimental research in developing practical quantum networking protocols.

PIKTime↗

Risk Characterization Research for Artemis II: Human Factors and Behavioral Performance

BACKGROUND Artemis II will be the first time NASA astronauts go beyond low-Earth orbit (LEO) since the Apollo era, and the first astronauts heading into space in the Orion vehicle. As such, it provides a critical opportunity to refine our understanding of the likelihood and consequences associated with the Behavioral Medicine (BMed), Team, Human System Integration Architecture (HSIA), and Sleep Risks, and prepare for future Moon and Mars missions. However, Artemis II research efforts are uniquely shaped by in-mission data collection constraints. There is currently no in-mission crew time available to complete measures. In-mission data will need to be collected unobtrusively from available data streams (e.g., audiovisual, existing records such as schedules, and actigraphy). Accordingly, the overarching goal of our research is to utilize Artemis II data to further define the likelihood and consequences of these risks, and to create an unobtrusive research infrastructure that can be expanded to include future Artemis missions. This goal spans four aims across three research phases: (1) identify and operationally define key performances metrics and constructs across the four aforementioned risks, (2) develop an unobtrusive methodology and coding scheme for in-mission data collection, (3) characterize performance decrements due to Bmed, Team, HSIA, and Sleep Risks, and (4) develop a data infrastructure for future Artemis missions. The following details results of Phase I efforts in which we address Aims 1 and 2 to develop an unobtrusive measurement plan and coding scheme to capture key constructs, contributing factors, and performance decrements across each risk area. METHOD As part of Phase I, we conducted an interdisciplinary literature review and consulted with SMEs to identify unobtrusive methodologies that leverage text, audio, and/or video data as well as conceptualize key performance metrics, contributing factors, and BMed, Team, HSIA, and Sleep risk constructs related to performance decrements. The Phase I effort resulted in a finalized pre- and post-mission protocol for Artemis II, along with a measurement and coding scheme for in-mission Artemis II data. Phase II will involve data collection from the upcoming Artemis II mission. Phase III will include data processing, coding, depiction, analysis, and report writing of the Artemis II data. RESULTS & DISCUSSION To date, we have completed Phase I efforts. Specifically, we identified BMed, Team, HSIA, and Sleep risk constructs related to performance metrics, summarized how these constructs can be measured using audiovisual data collected during the mission, and worked with NASA’s HFBP Element to finalize a data collection protocol that leverages audiovisual input from the Orion spacecraft system. Our protocol includes novel unobtrusive methodologies that adhere to in-mission data streams and subsequent constraints (e.g., limited storage space on GoPro cameras, ambient noise impeding audio files) to best capture in-mission phenomena across each risk area. We will present our results from Phase I efforts, namely best practices for unobtrusive measurement as identified through literature reviews and SME consultation as well as codebook excerpts for use in Artemis II. We will include a description of planned work as we prepare for Phase II and Phase III of this research plan and the Artemis II mission itself. SUMMARY We describe progress on our Human Factors and Behavioral Performance Research for Artemis II study.

behavioral health↗

Current Status of Martian Moons eXploration (MMX) Contamination Control and Curation Activity

Martian Moons eXploration (MMX) is a sample return mission from the Martian moon Phobos. The MMX spacecraft is scheduled to launch in 2026 and return to Earth in 2031. The main science goals of MMX are “to reveal the origin of the Martian moons and make progress in the understanding of planetary system formation and material transport in the solar system, and to observe processes that impact the circumplanetary and surface environments of Mars”. MMX has two sampling systems: coring (C)-sampler and pneumatic (P)-sampler and plans to bring back >10 g of Phobos sample. The retuned sample in the sample capsule will be transferred to the curation facility in ISAS/JAXA for sample curation and subsequent sample analysis. Contamination control of the sample return mission requires special care to prevent terrestrial contamination to the spacecraft, which would ruin the scientific value of the returned sample. Retaining the pristineness of the retuned sample is an important task of the MMX Curation and Sampler Science teams. The basis of the contamination control is (1) to minimize and understand the nature and amount of contaminants, (2) to perform contamination assessment and evaluate the effect of contaminants in the spacecraft on the retuned sample, (3) to employ a contamination knowledge (CK) material coupon in the spacecraft to identify the contaminants in the returned samples. In the MMX contamination control plan, the allowable contamination level for each contaminant is carefully defined. They are mostly set to be 1/1000 of the expected amount of each material in the returned sample and are divided into two main categories: organic and inorganic. The allowable atmospheric leakage rate to the sample container is also defined. The allowable contamination level of the organic materials is based on the composition of carbonaceous chondrites. The target contaminants are amino acids, aliphatic and aromatic hydrocarbons, carboxylic acids, etc. In case of the inorganic materials, the target contaminants are important elements to permit distinguishing the origin of the Martian moon by nucleosynthetic isotope anomalies (Cr, Ti, and Mo) and to reveal the evolution of the Martian moon by chronology (Hf, W, U, Pb, Rb, Sr, Sm, and Nd). The key instrument of contamination control in the sample return mission is the sampler system. The C-sampler has been developed by JAXA and the P-sampler was provided by Honeybee/NASA. In MMX, materials used in the two samplers (C- and P- sampler) were carefully selected to avoid potential contamination from the design stage of the system. The individual parts of the C-sampler FM (Flight Model) were thoroughly cleaned at the curation facility in ISAS/JAXA by the full-course cleaning procedure, which is an ultrasonic cleaning with organic solvents and ultrapure water in several steps. The equivalent level of cleaning was also carried out on the P-Samper FM as well by Honeybee Robotics in the USA. Now, MMX is in the critical phase for contamination control called ATLO: Assembly, Test, and Launch Operations. During the ATLO phase, sampler FM is constantly purged with nitrogen gas and maintained at positive pressure to prevent environmental contamination. The surrounding environments of the sampler FM are also simultaneously monitored using the CK Monitoring Coupon Set, which consists of several witness materials such as a glass petri dish, sapphire glass disk, and carbon adhesive tape (Figure 1). The detailed environmental assessment of each clean room used for the assembly and test of the sampler FM has also been conducted. This assessment includes microbial analysis, which was performed for OSIRIS-REx. Regarding the sample recovery and sample curation, we have started the designing of Sample Container Disassembling Instrument for the sample recovery from the sample container and the MMX curation chamber for sample curation. The curation protocol for the Phobos returned sample has also been discussed by the MMX Sample Analysis Working Team (SAWT). The MMX curation protocol consists of three phases: (1) quick analysis, (2) pre-basic characterization, and (3) basic characterization. (1) is extraction of the sample gas from the sample container and analysis by mass spectrometry, (2) is observation in bulk level, and (3) is observation in grain level and allocation of the sample aliquots. In parallel with the curation protocol, the returned sample undergoes preliminary examination for scientific investigations to achieve science goals. In addition, the CK witness plates made of sapphire glass are on board the sampler system. The CK witness plates will be recovered from the sampler system and analyzed by SAWT for the assessment of in-flight contamination.

Haruna Sugahara↗

Picosecond Clock Synchronization Across A 7-Node Metropolitan Scale Quantum Network

Quantum networking protocols relying on interference and precise time-of-flight measurements require high precision clock synchronization. This study describes the design, implementation, and characterization of two optical time transfer methods in a metropolitan-scale quantum networking research testbed. The first technique called active electronic stabilization (ELSTAB), was able to achieve sub-picosecond time deviation (TDEV) at integration times between 1 s and 105s over multiple different fiber lengths of both underground and aerial deployed fiber. The second technique, White Rabbit-Precision Time Protocol (WR-PTP), was able to achieve 10-picosecond TDEV’s over similar fiber lengths at comparable integration times. Environmental fluctuations affected the stability of clock synchronization over deployed fiber. These fluctuations resulted in path delay gradients, chromatic dispersion, polarization drift, and optical power variations. The results from this study will inform future work in the development of compensation methods essential for enabling experimental research in developing practical quantum networking protocols.

synchronization↗

Dry Saliva Development-Artemis

Prior to the deployment of prolonged deep space missions which may carry increased crew health risks, it is essential to determine the effect that missions beyond the Van Allen Belt will have on physiology. Historically the National Aeronautics and Space Administration (NASA), Human Research Program (HRP) has developed, conducted, and delivered research findings and countermeasures that will maintain the health and safety of crews aboard the International Space Station (ISS) in anticipation of future exploration class missions. To support these objectives, research operations on ISS typically include the collection and storage of human physiological samples and their return to Earth for analysis. Transition to Gateway and Artemis lunar exploration will limit the available up mass and biosample return capability resulting in new challenges to monitor crew health during the mission. The Artemis lunar missions provide a perfect opportunity to assess new technology that could support crew biosample return that is compatible with the severe operational constraints of Artemis mission design. Dried biosample chemistry analysis is a potential technology that can enable the collection of samples and tracking of crew health during these exploration class missions. The development and implementation of dried biosample chemistry technology for tracking immune health, viral reactivation and hormone fluctuations provide a simple alternative strategy for sample collection and sample return (light weight non-conditioned stowage option) for the continuation of human research during Artemis missions. Saliva is established as an informative biosample that has both its own unique available analytes as well as others that are also present in blood. Saliva is already routinely collected from ISS astronauts for the detection of stress hormones and latent virus DNA. The ‘Dry Saliva’ book sample collection/storage protocol, already successfully deployed to ISS, represents a perfect method for collecting biosamples from Gateway and Artemis astronauts because it requires a simple, non-invasive sample collection protocol, minimum volume, and uses non-conditioned storage. We are currently conducting an expanded stability study of the dry saliva analyte platform to include additional stress hormones, cytokines, antimicrobial proteins, and latent virus DNA as well as other markers of immunity and inflammation. Validation testing on parabolic flight will establish collection methods for saliva and blood to dry sampling substrates to finalize the collection protocols for flight. For the new expanded analyte stability study, a short-term stability study with time points out to 14 days has been completed with analysis underway to determine whether additional analytes could be recovered, as well as assessing the best storage conditions for the samples collected. In parallel, a long-term stability study is being conducted out to 1 year to improve the stability of some unstable analytes. To this end, the NASA Johnson Space Center’s Immunology/Virology lab is currently finalizing the saliva dry chemistry platform, including assay compatibility and stability studies. This technology is approved to be implemented, as part of the ‘Biomarkers’ study, during Artemis II through Artemis IV lunar missions.

Mayra Nelman-Gonzalez↗

Modeling the effects of exercise during 100% oxygen prebreathe on the risk of hypobaric decompression sickness

BACKGROUND: Several previous studies indicated that exercise during prebreathe with 100% O2 decreased the incidence of hypobaric decompression sickness (DCS). We report a meta-analysis of these investigations combined with a new study in our laboratory to develop a statistical model as a predictive tool for DCS. HYPOTHESIS: Exercise during prebreathe increases N2 elimination in a theoretical 360-min half-time compartment decreasing the incidence of DCS. METHODS: A dose-response probability tissue ratio (TR) model with 95% confidence limits was created for two groups, prebreathe with exercise (n = 113) and resting prebreathe (n = 113), using nonlinear regression analysis with maximum likelihood optimization. RESULTS: The model predicted that prebreathe exercise would reduce the residual N2 in a 360-min half-time compartment to a level analogous to that in a 180-min compartment. This finding supported the hypothesis. The incidence of DCS for the exercise prebreathe group was significantly decreased (Chi-Square = 17.1, p < 0.0001) from the resting prebreathe group. CONCLUSIONS: The results suggested that exercise during prebreathe increases tissue perfusion and N2 elimination approximately 2-fold and markedly lowers the risk of DCS. Based on the model, the prebreathe duration may be reduced from 240 min to a predicted 91 min for the protocol in our study, but this remains to be verified. The model provides a useful planning tool to develop and test appropriate prebreathe exercise protocols and to predict DCS risks for astronauts.

NASA Program Environmental Health↗

IPv6 and IPsec Tests of a Space-Based Asset, the Cisco Router in Low Earth Orbit (CLEO)

This report documents the design of network infrastructure to support testing and demonstrating network-centric operations and command and control of space-based assets, using IPv6 and IPsec. These tests were performed using the Cisco router in Low Earth Orbit (CLEO), an experimental payload onboard the United Kingdom – Disaster Monitoring Constellation (UKDMC) satellite built and operated by Surrey Satellite Technology Ltd (SSTL). On Thursday, 29 March 2007, NASA Glenn Research Center, Cisco Systems and SSTL performed the first configuration and demonstration of IPsec and IPv6 onboard a satellite in low Earth orbit. IPv6 is the next generation of the Internet Protocol (IP), designed to improve on the popular IPv4 that built the Internet, while IPsec is the protocol used to secure communication across IP networks. This demonstration was made possible in part by NASA’s Earth Science Technology Office (ESTO) and shows that new commercial technologies such as mobile networking, IPv6 and IPsec can be used for commercial, military and government space applications. This has direct application to NASA’s Vision for Space Exploration. The success of CLEO has paved the way for new space-based Internet technologies, such as the planned Internet Routing In Space (IRIS) payload at geostationary orbit, which will be a U.S. Department of Defense Joint Capability Technology Demonstration. This is a sanitized report for public distribution. All real addressing has been change to psueco addressing.

Communication↗

Technical Issues in Implementing DTN in a Flight Software Architecture

This slide presentation reviews some of the technical issues in implementing Delay Tolerant Networking (DTN) in a enviornments that lack continuous network connectivity, such as spacecraft in deepspace or submarines. In a DTN, asynchronous variable-length messages (called bundles) are routed in a store and forward manner between participating nodes over a heterogeneous network. The review examines the enabling technologies, the porting steps and issues, operational scenarios for DTN. There is a review of the Licklider Transmission Protocol (LTP) aka Long-haul Transmission Protocol. Also included is a brief review of the current uses of DTN.

Licklider Transmission Protocol (LTP)↗

Conclusion: Major Findings and Future Activities.

The Agricultural Model Intercomparison and Improvement Project (AgMIP) incorporates a number of major advances in the way that climate impacts, vulnerability, and adaptation are being simulated. At its core is a protocol approach that results in impact assessments being more scientifically credible and thus ultimately having greater value to the wide range of agricultural stakeholders. Moreover, the use of the protocol approach enables closer scrutiny and intercomparison of models and methods so that they can be improved over time. By creating a truly trans-disciplinary, systems-based approach, AgMIP impact assessments and evaluation of adaptations become useful to agricultural decision-makers at multiple scales. The chapters in this two-part set demonstrate the use of this approach and represent early steps towards the full realization of these new methods and their application.

damage assessment↗

Guide for Regional Integrated Assessments: Handbook of Methods and Procedures, Version 5.1

The purpose of this handbook is to describe recommended methods for a trans-disciplinary, systems-based approach for regional-scale (local to national scale) integrated assessment of agricultural systems under future climate, bio-physical and socio-economic conditions. An earlier version of this Handbook was developed and used by several AgMIP Regional Research Teams (RRTs) in Sub-Saharan Africa (SSA) and South Asia (SA)(AgMIP handbook version 4.2, www.agmip.org/regional-integrated-assessments-handbook/). In contrast to the earlier version, which was written specifically to guide a consistent set of integrated assessments across SSA and SA, this version is intended to be more generic such that the methods can be applied to any region globally. These assessments are the regional manifestation of research activities described by AgMIP in its online protocols document (available at www.agmip.org). AgMIP Protocols were created to guide climate, crop modeling, economics, and information technology components of its projects.

protocol (computers)↗

The Geostationary Operational Satellite R Series SpaceWire Based Data System

The Geostationary Operational Environmental Satellite R-Series Program (GOES-R, S, T, and U) mission is a joint program between National Oceanic & Atmospheric Administration (NOAA) and National Aeronautics & Space Administration (NASA) Goddard Space Flight Center (GSFC). SpaceWire was selected as the science data bus as well as command and telemetry for the GOES instruments. GOES-R, S, T, and U spacecraft have a mission data loss requirement for all data transfers between the instruments and spacecraft requiring error detection and correction at the packet level. The GOES-R Reliable Data Delivery Protocol (GRDDP) [1] was developed in house to provide a means of reliably delivering data among various on board sources and sinks. The GRDDP was presented to and accepted by the European Cooperation for Space Standardization (ECSS) and is part of the ECSS Protocol Identification Standard [2]. GOES-R development and integration is complete and the observatory is scheduled for launch November 2016. Now that instrument to spacecraft integration is complete, GOES-R Project reviewed lessons learned to determine how the GRDDP could be revised to improve the integration process. Based on knowledge gained during the instrument to spacecraft integration process the following is presented to help potential GRDDP users improve their system designs and implementation.

Networks↗

Technical Note: AQMEII4 Activity 1: Evaluation of Wet and Dry Deposition Schemes as an Integral Part of Regional-scale Air Quality Models

We present in this technical note the research protocol for phase 4 of the Air Quality Model Evaluation International Initiative (AQMEII4). This research initiative is divided into two activities, collectively having three goals: (i) to define the current state of the science with respect to representations of wet and especially dry deposition in regional models, (ii) to quantify the extent to which different dry deposition parameterizations influence retrospective air pollutant concentration and flux predictions, and (iii) to identify, through the use of a common set of detailed diagnostics, sensitivity simulations, model evaluation, and reduction of input uncertainty, the specific causes for the current range of these predictions. Activity 1 is dedicated to the diagnostic evaluation of wet and dry deposition processes in regional air quality models (described in this paper), and Activity 2 to the evaluation of dry deposition point models against ozone flux measurements at multiple towers with multiyear observations (to be described in future submissions as part of the special issue on AQMEII4). The scope of this paper is to present the scientific protocols for Activity 1, as well as to summarize the technical information associated with the different dry deposition approaches used by the participating research groups of AQMEII4. In addition to describing all common aspects and data used for this multi-model evaluation activity, most importantly, we present the strategy devised to allow a common process-level comparison of dry deposition obtained from models using sometimes very different dry deposition schemes. The strategy is based on adding detailed diagnostics to the algorithms used in the dry deposition modules of existing regional air quality models, in particular archiving diagnostics specific to land use–land cover (LULC) and creating standardized LULC categories to facilitate cross-comparison of LULC-specific dry deposition parameters and processes, as well as archiving effective conductance and effective flux as means for comparing the relative influence of different pathways towards the net or total dry deposition. This new approach, along with an analysis of precipitation and wet deposition fields, will provide an unprecedented process-oriented comparison of deposition in regional air quality models. Examples of how specific dry deposition schemes used in participating models have been reduced to the common set of comparable diagnostics defined for AQMEII4 are also presented.

Air Quality Model Evaluation International Initiat↗

Transforming any Facility for Meeting Strict Cleanliness Requirements

Over the life of the James Webb Space Telescope (JWST), Integration & Test (I&T) has taken place in areas that needed considerable work to make the facility itself and/or the protocols used while working in the rooms suitable to meet JWST percent area coverage (PAC) and molecular accumulation requirements. In addition to normal particulate matter, JWST had a uniquely significant challenge: fibers! Fibers not only cause much higher PAC levels, but they also risk damaging the angstrom sized Near Infrared Spectrometer (NIRSpec) microshutter array (MSA), which is critical to NIRSpec instrument performance. The primary emphasis of this paper is to address particulate and fiber contamination. The success of the JWST mission required effective cleanrooms, protocols, and mitigations in non-cleanroom areas that were pressed into service to house contamination-sensitive optics and scientific instruments. Some presented profound challenges. These included: NASA’s 60-year-old Johnson Space Center (JSC) Chamber A, which had never been used for anything contamination-sensitive, and the European tropical launch facilities, which were designed to meet International Standard Organization (ISO) Class 8 processing for communication satellites. The final challenge for JWST, as if to stare us in the face and say, “I dare you to try and make me clean enough,” was preparing the 4 areas in the Centre Spatial Guyanais (CSG) Final Assembly Building (BAF) located in French Guiana, a building in which one entire side opens for Ariane 5 rocket ingress and egress. This paper will describe our initial evaluation processes and the actual work undertaken to transform even the most challenging areas into first class cleanrooms that met JWST particulate and fiber requirements.

James Webb Space Telescope↗

Binary synchronous simulator

Flexible simulator for trouble-shooting data transmission system uses binary synchronous communications protocol to produce error-free transmission of data between two points. Protocol may be used to replace display generator or be directly fed to display generator.

Rogers, J. R., III↗