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At least 811 records · Page 45

Protein crystal growth in microgravity

The crystals of most proteins or other biological macromolecules are poorly ordered and diffract to lower resolutions than those observed for most crystals of simple organic and inorganic compounds. Crystallization in the microgravity environment of space may improve crystal quality by eliminating convection effects near growing crystal surfaces. A series of 11 different protein crystal growth experiments was performed on U.S. Space Shuttle flight STS-26 in September 1988. The microgravity-grown crystals of gamma-interferon D1, porcine elastase, and isocitrate lyase are larger, display more uniform morphologies, and yield diffraction data to significantly higher resolutions than the best crystals of these proteins grown on earth.

Delucas, Lawrence J.↗

Fluid mechanics of directional solidification at reduced gravity

The primary objective of the proposed research is to provide additional groundbased support for the flight experiment 'Casting and Solidification Technology' (CAST). This experiment is to be performed in the International Microgravity Laboratory-1 (IML-1) scheduled to be flown on a space shuttle mission scheduled for 1992. In particular, we will provide data on the convective motion and freckle formation during directional solidification of NH4Cl from its aqueous solution at simulated parameter ranges equivalent to reducing the gravity from the sea-level value down to 0.1 g or lower. The secondary objectives of the proposed research are to examine the stability phenomena associated with the onset of freckles and the mechanisms for their subsequent growth and decline (to eventual demise of some) by state-of-the-art imaging techniques and to formulate mathematical models for the prediction of the observed phenomena.

Chen, C. F.↗

Space Congress, 27th, Cocoa Beach, FL, Apr. 24-27, 1990, Proceedings

The present symposium on aeronautics and space encompasses DOD research and development, science payloads, small microgravity carriers, the Space Station, technology payloads and robotics, commercial initiatives, STS derivatives, space exploration, and DOD space operations. Specific issues addressed include the use of AI to meet space requirements, the Astronauts Laboratory Smart Structures/Skins Program, the Advanced Liquid Feed Experiment, an overview of the Spacelab program, the Autonomous Microgravity Industrial Carrier Initiative, and the Space Station requirements and transportation options for a lunar outpost. Also addressed are a sensor-data display for telerobotic systems, the Pegasus and Taurus launch vehicles, evolutionary transportation concepts, the upgrade of the Space Shuttle avionics, space education, orbiting security sentinels, and technologies for improving launch-vehicle responsiveness.

Source record↗

Scaling analysis of gas-liquid two-phase flow pattern in microgravity

A scaling analysis of gas-liquid two-phase flow pattern in microgravity, based on the dominant physical mechanism, was carried out with the goal of predicting the gas-liquid two-phase flow regime in a pipe under conditions of microgravity. The results demonstrated the effect of inlet geometry on the flow regime transition. A comparison of the predictions with existing experimental data showed good agreement.

Lee, Jinho↗

Medical and surgical applications of space biosensor technology

Researchers in space life sciences are rapidly approaching a technology impasse. Many of the critical questions on the impact of spaceflight on living systems simply cannot be answered with the limited available technologies. Research subjects, particularly small animal models like the rat, must be allowed to function relatively untended and unrestrained for long periods to fully reflect the impact of microgravity and spaceflight on their behavior and physiology. These requirements preclude the use of present hard-wired instrumentation techniques and limited data acquisition systems. Implantable sensors and miniaturized biotelemetry are the only means of capturing the fundamental and critical data. This same biosensor and biotelemetry technology has direct application to Earth-based medicine and surgery. Continuous, on-line data acquisition and improved measurement capabilities combined with the ease and flexibility offered by automated, wireless, and portable instruments and data systems, should provide a boon to the health care industry. Playing a key role in this technology revolution is the Sensors 2000! (S2K!) Program at NASA Ames Research Center. S2K!, in collaboration with space life sciences researchers and managers, provides an integrated capability for sensor technology development and applications, including advanced biosensor technology development, spaceflight hardware development, and technology transfer and commercialization. S2K! is presently collaborating on several spaceflight projects with dual-use medical applications. One prime example is a collaboration with the Fetal Treatment Center (FTC) at the University of California at San Francisco. The goal is to develop and apply implantable chemical sensor and biotelemetry technology to continuously monitor fetal patients during extra-uterine surgery, replacement into the womb, through birth and beyond. Once validated for ground use, the method will be transitioned to spaceflight applications to remotely monitor key biochemical parameters in flight animals. Successful application of NASA implantable biosensor and biotelemetry technologies should accelerate the advancement of this and other modern medical procedures while furthering the exploration of life in space.

NASA Discipline General Space Life Sciences↗

Mechanics of Granular Materials (MGM) Microgravity Experiment

The second series of MGM experiment was conducted during the STS-89 mission in January 1998. The experiment was previously flow on Atlantis's STS-79 mission in September 1996. Six displacement-controlled, drained triaxial compression experiments were performed at very low effective confining stresses. The confining stresses were in the ranges 0.05, 0.52 and 1.30 kPa. Three experiments were subjected to monotonic loading and unloading cycles while the other three experiments were subjected to cyclic loading. The results show very high peak strength friction angles in the range of 47.6 to 70.0 degrees, which are mainly due to overconsolidation and grain interlocking effects. It was observed that the residual strength levels in the monotonic loading experiments were in the same range as that observed at higher confining stress levels. The dilatancy angles were unusually high in the range of 30 to 31 degrees. All specimens display substantial initial stiffnesses and elastic moduli during unloading and reloading events, which are nearly an order of magnitude higher than conventional theories predict. A periodic instability phenomenon which appears to result from buckling of multiple internal arches and columnar systems, augmented by stick-slips was observed in the experiments. Computed Tomography (CT) measurements revealed valuable data about the internal fabric and the specimens deformation patterns. Uniform diffuse bifurcation with multiple radial shear bands was observed in the specimens tested in a microgravity environment. In the axial direction, two major conical surfaces were developed. Spatial nonsymmetrical deformations were observed in specimens tested in terrestrial laboratory.

Alshibli, Khalid A.↗

Microgravity nucleation and particle coagulation experiments support

A hollow sphere model is developed to predict the range of supersaturation ratio values for refractory metal vapors in a proposed experimental nucleation apparatus. Since the experiments are to be carried out in a microgravity environment, the model neglects the effects of convection and assumes that the only transfer of vapors through an inert gas atmosphere is by conduction and molecular diffusion. A consistent set of physical properties data is assembled for the various candidate metals and inert ambient gases expected to be used in the nucleation experiments. Transient partial pressure profiles are computed for the diffusing refractory species for two possible temperature distributions. The supersaturation ratio values from both candidate temperature profiles are compared with previously obtained experimetnal data on a silver-hydrogen system. The model is used to simulate the diffusion of magnesium vapor through argon and other inert gas atmospheres over ranges of initial and boundary conditions. These results identify different combinations of design and operating parameters which are liekly to produce supersaturation ratio values high enough to induce homogeneous nucleation in the apparatus being designed for the microgravity nucleation experiments.

Lilleleht, L. U.↗

NASA Lewis' Telescience Support Center Supports Orbiting Microgravity Experiments

The Telescience Support Center (TSC) at the NASA Lewis Research Center was developed to enable Lewis-based science teams and principal investigators to monitor and control experimental and operational payloads onboard the International Space Station. The TSC is a remote operations hub that can interface with other remote facilities, such as universities and industrial laboratories. As a pathfinder for International Space Station telescience operations, the TSC has incrementally developed an operational capability by supporting space shuttle missions. The TSC has evolved into an environment where experimenters and scientists can control and monitor the health and status of their experiments in near real time. Remote operations (or telescience) allow local scientists and their experiment teams to minimize their travel and maintain a local complement of expertise for hardware and software troubleshooting and data analysis. The TSC was designed, developed, and is operated by Lewis' Engineering and Technical Services Directorate and its support contractors, Analex Corporation and White's Information System, Inc. It is managed by Lewis' Microgravity Science Division. The TSC provides operational support in conjunction with the NASA Marshall Space Flight Center and NASA Johnson Space Center. It enables its customers to command, receive, and view telemetry; monitor the science video from their on-orbit experiments; and communicate over mission-support voice loops. Data can be received and routed to experimenter-supplied ground support equipment and/or to the TSC data system for display. Video teleconferencing capability and other video sources, such as NASA TV, are also available. The TSC has a full complement of standard services to aid experimenters in telemetry operations.

Hawersaat, Bob W.↗

Studies of Two-Phase Gas-Liquid Flow in Microgravity

Two-phase gas-liquid flows are expected to occur in many future space operations. Due to a lack of buoyancy in the microgravity environment, two-phase flows are known to behave differently than those in earth gravity. Despite these concerns, little research has been conducted on microgravity two-phase flow and the current understanding is poor. This dissertation describes an experimental and modeling study of the characteristics of two-phase flows in microgravity. An experiment was operated onboard NASA aircraft capable of producing short periods of microgravity. In addition to high speed photographs of the flows, electronic measurements of void fraction, liquid film thickness, bubble and wave velocity, pressure drop and wall shear stress were made for a wide range of liquid and gas flow rates. The effects of liquid viscosity, surface tension and tube diameter on the behavior of these flows were also assessed. From the data collected, maps showing the occurrence of various flow patterns as a function of gas and liquid flow rates were constructed. Earth gravity two-phase flow models were compared to the results of the microgravity experiments and in some cases modified. Models were developed to predict the transitions on the flow pattern maps. Three flow patterns, bubble, slug and annular flow, were observed in microgravity. These patterns were found to occur in distinct regions of the gas-liquid flow rate parameter space. The effect of liquid viscosity, surface tension and tube diameter on the location of the boundaries of these regions was small. Void fraction and Weber number transition criteria both produced reasonable transition models. Void fraction and bubble velocity for bubble and slug flows were found to be well described by the Drift-Flux model used to describe such flows in earth gravity. Pressure drop modeling by the homogeneous flow model was inconclusive for bubble and slug flows. Annular flows were found to be complex systems of ring-like waves and a substrate film. Pressure drop was best fitted with the Lockhart- Martinelli model. Force balances suggest that droplet entrainment may be a large component of the total pressure drop.

Bousman, William Scott↗

The Effects of Gravity on Wrinkled Laminar Flames

The effects of gravity are significant to the dynamics of idealized unconfined open premixed flames. Moderate to low turbulence Reynolds number flames, i.e., wrinkled laminar flames, of various unconfined geometries have been used extensively for investigating fundamental processes of turbulent flame propagation and to validate theoretical models. Without the wall constraints, the flames are free to expand and interact with surrounding ambient air. The flow field in which the flame exists is determined by a coupling of burner geometry, flame orientation and the gravity field. These complex interactions raise serious questions regarding the validity of comparing the experimental data of open flames with current theoretical and numerical models that do not include the effects of gravity nor effects of the larger aerodynamic flowfield. Therefore, studies of wrinkled laminar flame in microgravity are needed for a better understanding of the role of gravity on flame characteristics such as the orientation, mean aerodynamics stretch, flame wrinkle size and burning rate. Our approach to characterize and quantify turbulent flame structures under microgravity is to exploit qualitative and quantitative flow visualization techniques coupled with video recording and computer controlled image analysis technologies. The experiments will be carried out in the 2.2 second drop tower at the NASA Lewis Research Center. The longest time scales of typical wrinkled laminar flames in the geometries considered here are in the order of 10 msec. Hence, the duration of the drop is sufficient to obtain the amount of statistical data necessary for characterize turbulent flame structures.

Kostiuk, Larry W.↗

KSC to EDEN ISS: Outredgeous Lettuce Growth on the Passive Porous Tube Nutrient Delivery System (PPTNDS)

EDEN ISS is a controlled environment greenhouse established near the Neumayer Station III in Antarcticawith the mission to enable fresh crop production for the crew who are working in this remote location. EDEN provides a space to conduct plant biology and related research in a unique and isolated controlled environment (CE), while providing a platform to test developing methods relevant to long duration manned space missions. In 2021, Kennedy Space Center (KSC) scientist Jess Bunchek fulfilled a one-year mission at EDEN. During that time a variety of fresh crops were grown to record horticultural and microbiological data as well as provide the crew with fresh food to eat. One method for crop growth included an experimental irrigation system called the Passive Porous Tube Nutrient Delivery System (PPTNDS) which was developed at KSC as a microgravity-capable plant irrigation system designed to support Space Plant Biology investigations and/or crop growth during space missions. The PPTNDS was employed to grow Outredgeous Lettuce (OL) during two growouts at EDEN, (harvesting at Day After Planting (DAP) 61 and 63) and two growouts at KSC where plant growth and micro sample data were captured. This paper presents the data collected from the PPTNDS and compares it to growth on a Nutrient Film Technique (NFT) hydroponic system operated in the same environment. The result shows a system that grows OL at a reduced rate compared to the NFT system but uses less water and crew interaction to operate.

Jacob Torres↗

A Review and Comparison of Mouse and Rat Responses to Micro Gravity, Hyper Gravity and Simulated Models of Partial Gravity; Species Differences, Gaps in the Available Data, and Consideration of the Advantages and Caveats of Each Model for Spaceflight

Laboratory strains of mice and rat are widely used to study mammalian responses to stimulus, and both have been studied under a variety of gravity conditions, including space flight. We compared results obtained from exposure to spaceflight and microgravity, hyper gravity via centrifugation, earth gravity, and models of simulated partial gravity (hind-limb unloading and partial weight bearing treatments). We examined the reported changes in survival, body mass, circadian rhythm (body temperature and activity levels), behavior, bone, muscle, immune, cardio-vasculature, vestibular, reproduction and neonate survival, microbiome, and the visual system. Not all categories have published data for both species, some have limited data, and there are variations in experiment design that allow for only relative comparisons to be considered. The data reveal species differences in both the level of gravity required to obtain a response, degree of response, and in temporal expression of responses. Examination of the data across the gravity levels allows consideration of the hypothesis that gravitational responses follow a continuum, and organ specific differences are noted. In summary, we present advantages and caveats of each model system as pertains to gravitational biology research and identify gaps in our knowledge of how these mammals respond to gravity.

mouse rat gravity↗

Parabolic Flight Investigation for Advanced Exercise Concept Hardware Hybrid Ultimate Lifting Kit (HULK)

Long-duration space flight poses many hazards to the health of the crew. Among those hazards is the physiological deconditioning of the musculoskeletal and cardiovascular systems due to prolonged exposure to microgravity. To combat this erosion of physical condition space flight may take on the crew, the Human Research Program (HRP) is charged with developing Advanced Exercise Concepts to maintain astronaut health and fitness during long-term missions, while keeping device mass, power, and volume to a minimum. The goal of this effort is to preserve the physical capability of the crew to perform mission critical tasks in transit and during planetary surface operations. The HULK is a pneumatic-based exercise system, which provides both resistive and aerobic modes to protect against human deconditioning in microgravity. Its design targeted the International Space Station (ISS) Advanced Resistive Exercise Device (ARED) high level performance characteristics and provides up to 600 foot pounds resitive loading with the capability to allow for eccentric to concentric (E:C) ratios of higher than 1:1 through a DC motor assist component. The device's rowing mode allows for high cadence aerobic activity. The HULK parabolic flight campaign, conducted through the NASA Flight Opportunities Program at Ellington Field, resulted in the creation of device specific data sets including low fidelity motion capture, accelerometry and both inline and ground reaction forces. These data provide a critical link in understanding how to vibration isolate the device in both ISS and space transit applications. Secondarily, the study of human exercise and associated body kinematics in microgravity allows for more complete understanding of human to machine interface designs to allow for maximum functionality of the device in microgravity.

countermeasures↗

A Study of Nucleate Boiling with Forced Convection in Microgravity

The ultimate objective of basic studies of flow boiling in microgravity is to improve the understanding of the processes involved, as manifested by the ability to predict its behavior. This is not yet the case for boiling heat transfer even in earth gravity, despite the considerable research activity over the past 30 years. The elements that constitute the nucleate boiling process - nucleation, growth, motion, and collapse of the vapor bubbles (if the bulk liquid is subcooled) - are common to both pool and flow boiling. It is well known that the imposition of bulk liquid motion affects the vapor bubble behavior relative to pool boiling, but does not appear to significantly influence the heat transfer. Indeed, it has been recommended in the past that empirical correlations or experimental data of pool boiling be used for design purposes with forced convection nucleate boiling. It is anticipated that such will most certainly not be possible for boiling in microgravity, based on observations made with pool boiling in microgravity. In earth gravity buoyancy will act to remove the vapor bubbles from the vicinity of the heater surface regardless of how much the imposed bulk velocity is reduced, depending, of course, on the geometry of the system. Vapor bubbles have been observed to dramatically increase in size in pool boiling in microgravity, and the heat flux at which dryout took place was reduced considerably below what is generally termed the critical heat flux (CHF) in earth gravity, depending on the bulk liquid subcooling. However, at heat flux levels below dryout, the nucleate pool boiling process was enhanced considerably over that in earth gravity, in spite of the large vapor bubbles formed in microgravity and perhaps as a consequence. These large vapor bubbles tended to remain in the vicinity of the heater surface, and the enhanced heat transfer appeared to be associated with the presence of what variously has been referred to as a liquid microlayer between the bubble and the heater surface. The enhancement of the boiling process with low velocities in earth gravity for those orientations producing the formation of a liquid macrolayer described above, accompanied by "sliding" vapor bubbles, has been demonstrated. The enhancement was presented as a function of orientation, subcooling, and heated length, while a criterion for the heat transfer for mixed natural/forced convection nucleate boiling was given previously. A major unknown in the prediction and application of flow boiling heat transfer in microgravity is the upper limit of the heat flux for the onset of dryout (or critical heat flux - CHF), for given conditions of fluid-heater surfaces, including geometry, system pressure and bulk liquid subcooling. It is clearly understood that the behavior in microgravity will be no different than on earth with sufficiently high flow velocities, and would require no space experimentation. However, the boundary at which this takes place is still an unknown. Previous results of CHF measurements were presented for low velocity flow boiling at various orientations in earth gravity as a function of flow velocity and bulk liquid subcooling, along with preliminary measurements of bubble residence times on a flat heater surface. This showed promise as a parameter to be used in modeling the CHF, both in earth gravity and in microgravity. The objective of the work here is to draw attention to and show results of current modeling efforts for the CHF, with low velocities in earth gravity at different orientations and subcoolings. Many geometrical possibilities for a heater surface exist in flowing boiling, with boiling on the inner and outer surfaces of tubes perhaps being the most common. If the vapor bubble residence time on and departure size from the heater surface bear a relationship to the CHF, as results to be given indicate, it is important that visualization of and access to vapor bubble growth be conveniently available for research purposes. In addition, it is desirable to reduce the number of variables as much as possible in a fundamental study. These considerations dictated the use of a flat heater surface, which is rectangular in shape, 1.91 cm by 3.81 cm (0.75 x 1.5 inches), consisting either of a 400 Angstrom thick semi-transparent gold film sputtered on a quartz substrate which serves simultaneously as a heater and a resistance thermometer, or a copper substrate of the same size. The heater substrate is a disc which can be rotated so that the heated length in the flow direction can be changed from 1.91 to 3.81 cm (0.75 to 1.5 inches). The fluid is R-113, and the velocities can be varied between 0.5 cm/s and 60 cm/s. For a sufficiently low velocity the CHF can be modeled reasonably well at various orientations by the correlation for pool boiling corrected for the influence of bulk liquid subcooling, multiplied by the square root of q, the angle relative to horizontal. This arises from equating buoyancy and drag forces in the inverted positions where the vapor bubbles are held against the heater surface as they slide. A distortion of the measurements relative to pool boiling occurs as the flow velocity increases. In modeling this effect at different levels of subcooling it appeared appropriate to estimate the volumetric rate of vapor generation, using measurements of bubble frequency (or residence time), void fraction and average bubble boundary layer thickness. These were determined with the use of a platinum hot wire probe 0.025 mm in diameter by 1.3 mm long, applying a constant current to distinguish between contact with liquid or vapor. Two-dimensional spatial variations are obtained with a special mechanism to resolve displacements in increments of 0.025 mm. From such measurements it was determined that the fraction of the surface heat transfer resulting in evaporation varies inversely with the subcooling correction factor for the CHF. The measured inverse bubble residence time is normalized relative to that predicted for an infinite horizontal flat plate at the CHF, and is correlated well with the CHF normalized relative to that for pool boiling, for various orientation angles and subcooling levels. This correspondence is then combined with a normalizing factor for the energy flux leaving the heater surface at the CHF and the computed bubble radius at departure, determined from the balance between the outward velocity of the interface due to evaporation and the buoyance induced velocity of the center of mass of the bubble. The product of the CHF and the corresponding residence time was determined to be a constant for all orientations at a given bulk flow velocity and liquid subcooling, and must be determined empirically for each velocity and subcooling at present. It then becomes possible to predict the CHF for the different orientations, velocities, and subcoolings. These are compared with normalized measurements of the CHF for velocities ranging from 4 cm/s to 55 cm/s, subcoolings from 2.8 to 22.2 K, over orientations angles of 360 degrees.

Merte, Herman, Jr.↗

Cross Kingdom Analysis of Data Within the GeneLab Repository Identifies a Potential Conserved Response of Life to the Stress Associated with Spaceflight

It is important to determine the health risks and potential survival for astronauts associated with long-term space missions. This entails not only understanding the impact the space environment will have on humans, but also how it will affect other organisms needed for humans to survive in space such as plants. In addition, it has been reported in the literature that hundreds of genes seem to be conserved and/or transferred between different organisms from bacteria, archaea, fungi, microorganisms, and plants to animals. Since space travel involves humans in a closed environment over a long period of time, we hypothesize that potential conserved biological factors will occur between the different organisms in that environment possibly due to transfer of genes. Determining the conserved factors that are commonly being regulated in space can shed insight into possible universal master regulators and also determine the symbiotic relationship between the organisms in space. Utilizing NASA's GeneLab Data Repository (a rapidly expanding, curated clustering of spaceflight-related ‘omics-level datasets for all organisms), we were able to uncover a novel pathway and factors that were commonly shared between humans, mice, plants, C. Elegans, and drosophilas. Through ChIP-Seq enrichment analysis techniques utilizing various GeneLab datasets from each species that were flown in space, we found the following factors to be conserved across all species: oxidative stress, DNA damage (through GABPA/NRFs and NFY), SIX5, GTF2B and glutamine synthetase. Such commonalities would likely reflect the effects of factors such as microgravity and the increased radiation exposure inherent in spaceflight on basic physical processes shared by all biological systems at the cellular level. Differences between organismal responses revealed by GeneLab's data should also help understand the unique reactions to life in space that arise from the very different lifestyles of microbes, animals and plants.

Barker, Richard↗

Constitutive and Stability Behavior of Granular Materials Under Very Low Effective Stresses

The study of the behavior of granular materials such as sand is of great importance to the understanding of the response of foundations resting on surface and near-surface soil deposits. A clear understanding of the behavior of such materials can also provide insights into other related issues where low effective stresses are encountered such as liquefaction. The main sources of the constitutive and stability properties of cohesionless granular materials is interparticle friction, which in turn under low confinement stress levels is strongly affected by gravitational body forces under terrestrial (1 gravity) conditions. Under moderate-to-high stress levels, the influence of gravity on the behavior of experiments may not be pronounced and therefore the test results in a terrestrial environment may be acceptable for engineering purposes. However, the conduct of experiments on granular materials under very low stress levels and under quasi-static conditions can only be performed in a microgravity environment. A series of displacement-controlled cyclic triaxial compression experiments were performed in a SPACEHAB module on the Space Shuttle during the STS-79 mission to Mir in September, 1996, and the STS-89 mission in January, 1998. The experiments were conducted on six right cylindrical specimens 75 mm in diameter and 150 mm long at effective confining pressures of 0.05, 0.52 and 1.30 kPa. The results show very high peak strength friction angles in the range of 47.6 to 70.0 degrees, which are mainly due to overconsolidation and grain interlocking effects. It was observed that the residual strength levels were in the same range as that observed at higher confining stress levels. The dilatancy angles were unusually high in the range of 30 to 31 degrees. All specimens display substantial initial stiffnesses and elastic moduli during unloading and reloading events, which are nearly an order of magnitude higher than conventional theories predict. A periodic instability phenomenon which appears to result from buckling of multiple internal arches and columnar systems, augmented by stick-slips was observed in the experiments. Computed Tomography (CT) measurements revealed valuable data about the internal fabric and the specimens deformation patterns. Uniform diffuse bifurcation with multiple radial shear bands was observed in the specimens tested in a microgravity environment. In the axial direction, two major conical surfaces were developed. Spatial nonsymmetrical deformations were observed in specimens tested in terrestrial laboratory.

Alshibli, Khalid A.↗

Bioavailability and Pharmacodynamics of Promethazine in Human Subjects

Space Motion Sickness (SMS) is often treated in space with promethazine (PMZ). Anecdotal reports indicate that the common side effects of drowsiness and decrements in cognitive performance that are associated with PMZ administration (50 mg IM on the ground, are absent or less pronounced in space suggesting I that-the bioavailability and/or pharmacodynamic behavior of PMZ may be altered during space flight. There are limited flight opportunities available for clinical research in space, the NRA-99, therefore, solicits research required to improve, or answer specific questions about in-flight diagnosis, therapy, and post-flight rehabilitation. We propose here, to establish a noninvasive method for pharmacodynamic and therapeutic assessment of PMZ. The specific objectives of the proposed research are to, 1. Establish a saliva to plasma ratio of PMZ after administration, 2. Estimate the relative bioavailability of the three flight-specific dosage forms of PMZ, and 3. Establish the dose-response relationship of PMZ. We will estimate the bioavailability of intramuscular injection (IM), oral tablets and rectal suppositories in normal subjects during ambulatory and antiorthostatic; bed rest (ABR) conditions using novel stable isotope techniques. Drowsiness, cognitive performance and salivary flow rate will be measured as a function of circulating drug concentrations after administration of three IM doses of PMZ. We will compare and contrast the bioavailability of PMZ during normal and ABR conditions to examine whether or not ABR can simulate changes in drug, absorption and availability similar to those anticipated in a microgravity environment. Results of this study will validate methods for an approved study with this medication awaiting a flight opportunity for manifestation. These data will also provide the much needed information on the dynamics and therapeutic index. of this medication and their implications on crew fatigue and performance in space. Key words: Promethazine, stable isotopes, bioavailability, pharmacodynamics, cognitive performance, antiorthostatic bed rest.

Putcha, Lakshmi↗

Biological Dosimetry in Astronauts

Biodosimetry data provides a direct measurement of space radiation damage, which takes into account individual radiosensitivity in the presence of confounding factors such as microgravity and other stress conditions. In contrast to physical measurements, which are external to body and require multiple devices to detect all radiation types all of which have poor sensitivity to neutrons, biodosimetry is internal and includes the effects of shielding provided by the body itself plus chromosome damage shows excellent sensitivity to protons, heavy ions, and neutrons. Moreover, chromosome damage maybe reflective of cancer risk and biodosimetry values can therefore be used to validate and develop risk assessment models that can be used to characterize excess health risk incurred by crewmembers. Cytogenetic biodosimetry methods have been used extensively for assessing terrestrial radiation exposures, and remain the most sensitive in vivo indicator of dose available to date. The main cellular radiation target is the DNA, and radiation-induced damage in the DNA molecule can be visualized as aberrations in the chromosomes (breaks in the chromosomes or exchanges of DNA material between different chromosomes). Normal chromosomes contain a single condensed and constricted area called a centromere that helps the chromosome number to remain stable when a cell divides.

George, Kerry↗