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Evolutionary flexibility and rigidity in the bacterial methylerythritol phosphate (MEP) pathway

Terpenoids are a diverse class of compounds with wide-ranging uses including as industrial solvents, pharmaceuticals, and fragrances. Efforts to produce terpenoids sustainably by engineering microbes for fermentation are ongoing, but industrial production still largely relies on nonrenewable sources. The methylerythritol phosphate (MEP) pathway generates terpenoid precursor molecules and includes the enzyme Dxs and two iron–sulfur cluster enzymes: IspG and IspH. IspG and IspH are rate limiting-enzymes of the MEP pathway but are challenging for metabolic engineering because they require iron–sulfur cluster biogenesis and an ongoing supply of reducing equivalents to function. Therefore, identifying novel alternatives to IspG and IspH has been an on-going effort to aid in metabolic engineering of terpenoid biosynthesis. We report here an analysis of the evolutionary diversity of terpenoid biosynthesis strategies as a resource for exploration of alternative terpenoid biosynthesis pathways. Using comparative genomics, we surveyed a database of 4,400 diverse bacterial species and found that some may have evolved alternatives to the first enzyme in the pathway, Dxs making it evolutionarily flexible. In contrast, we found that IspG and IspH are evolutionarily rigid because we could not identify any species that appear to have enzymatic routes that circumvent these enzymes. The ever-growing repository of sequenced bacterial genomes has great potential to provide metabolic engineers with alternative metabolic pathway solutions. With the current state of knowledge, we found that enzymes IspG and IspH are evolutionarily indispensable which informs both metabolic engineering efforts and our understanding of the evolution of terpenoid biosynthesis pathways.

59 BASIC BIOLOGICAL SCIENCES↗

X-Plane Knowledge Capture Workshop Summary Report

The history of experimental aircraft research and development at the National Aeronautics and Space Administration (NASA) provides a large body of work for current and future team members to learn from and apply. NASA values the knowledge it gains from all the research it performs and desires to have that knowledge grow with each new project, incrementally adding to the collective knowledge base. To support this knowledge growth and sharing, in May 2019, NASA convened over 60 engineers; researchers; and other personnel (internal and external to NASA) for the X-plane1 Knowledge Capture Workshop to discuss their over 50-years’ worth of experiences in developing, operating, and managing experimental aircraft. Over the course of a two-day workshop, the group not only focused, primarily, on current X-plane development but also discussed a wide range of topics about experimental aircraft and flight research. In addition to the discussions, senior NASA aeronautics leaders shared their personal lessons learned with the workshop audience. Broadly, these discussions centered around the topics of organizational and cultural practices, the project lifecycle, flight, and risk and safety. Many of the observations offered by participants are applicable to other NASA team-based projects, demonstrating the value in sharing lessons learned and best practices across projects regardless of their research focus. To share the most valuable lessons learned as broadly as possible, a summary of these discussions has been compiled into the following report. These discussions are mostly included in their entirety, with some light editing for clarity and privacy. NASA is sharing this knowledge internally and making it available to the public in hopes that others can learn from the experimental aircraft challenges and successes at NASA. Note: this document represents the perspectives, experiences, and opinions of others but does not necessarily represent the official view of NASA.

Steven R Hirshorn↗

Evolutionary Flexibility and Rigidity in the Bacterial Methylerythritol Phosphate (Mep) Pathway

Terpenoids are a diverse class of compounds with wide-ranging uses including as industrial solvents, pharmaceuticals, and fragrances. Efforts to produce terpenoids sustainably by engineering microbes for fermentation are ongoing, but industrial production still largely relies on nonrenewable sources. The methylerythritol phosphate (MEP) pathway generates terpenoid precursor molecules and includes the enzyme Dxs and two iron–sulfur cluster enzymes: IspG and IspH. IspG and IspH are rate limiting-enzymes of the MEP pathway but are challenging for metabolic engineering because they require iron–sulfur cluster biogenesis and an ongoing supply of reducing equivalents to function. Therefore, identifying novel alternatives to IspG and IspH has been an on-going effort to aid in metabolic engineering of terpenoid biosynthesis. We report here an analysis of the evolutionary diversity of terpenoid biosynthesis strategies as a resource for exploration of alternative terpenoid biosynthesis pathways. Using comparative genomics, we surveyed a database of 4,400 diverse bacterial species and found that some may have evolved alternatives to the first enzyme in the pathway, Dxs making it evolutionarily flexible. In contrast, we found that IspG and IspH are evolutionarily rigid because we could not identify any species that appear to have enzymatic routes that circumvent these enzymes. The ever-growing repository of sequenced bacterial genomes has great potential to provide metabolic engineers with alternative metabolic pathway solutions. With the current state of knowledge, we found that enzymes IspG and IspH are evolutionarily indispensable which informs both metabolic engineering efforts and our understanding of the evolution of terpenoid biosynthesis pathways.

Bailey Marshall↗

Establishment of a National Wind Energy Center at University of Houston

The DOE-supported project objectives are to: establish a national wind energy center (NWEC) at University of Houston and conduct research to address critical science and engineering issues for the development of future large MW-scale wind energy production systems, especially offshore wind turbines. The goals of the project are to: (1) establish a sound scientific/technical knowledge base of solutions to critical science and engineering issues for developing future MW-scale large wind energy production systems, (2) develop a state-of-the-art wind rotor blade research facility at the University of Houston, and (3) through multi-disciplinary research, introducing technology innovations on advanced wind-turbine materials, processing/manufacturing technology, design and simulation, testing and reliability assessment methods related to future wind turbine systems for cost-effective production of offshore wind energy. To achieve the goals of the project, the following technical tasks were planned and executed during the period from April 15, 2010 to October 31, 2014 at the University of Houston: (1) Basic research on large offshore wind turbine systems (2) Applied research on innovative wind turbine rotors for large offshore wind energy systems (3) Integration of offshore wind-turbine design, advanced materials and manufacturing technologies (4) Integrity and reliability of large offshore wind turbine blades and scaled model testing (5) Education and training of graduate and undergraduate students and post- doctoral researchers (6) Development of a national offshore wind turbine blade research facility The research program addresses both basic science and engineering of current and future large wind turbine systems, especially offshore wind turbines, for MW-scale power generation. The results of the research advance current understanding of many important scientific issues and provide technical information for solving future large wind turbines with advanced design, composite materials, integrated manufacturing, and structural reliability and integrity. The educational program have trained many graduate and undergraduate students and post-doctoral level researchers to learn critical science and engineering of wind energy production systems through graduate-level courses and research, and participating in various projects in center’s large multi-disciplinary research. These students and researchers are now employed by the wind industry, national labs and universities to support the US and international wind energy industry. The national offshore wind turbine blade research facility developed in the project has been used to support the technical and training tasks planned in the program to accomplish their goals, and it is a national asset which is available for used by domestic and international researchers in the wind energy arena.

17 WIND ENERGY↗

RT-Syn: A real-time software system generator

This paper presents research into providing highly reusable and maintainable components by using automatic software synthesis techniques. This proposal uses domain knowledge combined with automatic software synthesis techniques to engineer large-scale mission-critical real-time software. The hypothesis centers on a software synthesis architecture that specifically incorporates application-specific (in this case real-time) knowledge. This architecture synthesizes complex system software to meet a behavioral specification and external interaction design constraints. Some examples of these external constraints are communication protocols, precisions, timing, and space limitations. The incorporation of application-specific knowledge facilitates the generation of mathematical software metrics which are used to narrow the design space, thereby making software synthesis tractable. Success has the potential to dramatically reduce mission-critical system life-cycle costs not only by reducing development time, but more importantly facilitating maintenance, modifications, and extensions of complex mission-critical software systems, which are currently dominating life cycle costs.

Setliff, Dorothy E.↗

Using Knowledge Analytics to Search and Characterize Mass Properties Aerospace Data

There is growing capability in the field of “Big Data” and “Data Analytics” which Mass Properties Engineers might like to take advantage of. This paper utilizes an implementation of the IBM Knowledge Analytics and Watson search capabilities to explore a corpus of material developed primarily with the interests of Mass Properties Engineers and vehicle concept developers at its forefront. The full collection of SAWE (Society of Allied Weight Engineers, Inc.) Technical Papers from 1939 through 2015 is a major portion of the knowledge content. Additional aerospace vehicle design information includes metadata from AIAA (American Institute for Aeronautics and Astronautics), and INCOSE (International Council on Systems Engineering) as well as author-provided personal search material. This data is processed with respect to certain expected content, data taxonomies and key words to become the core data in NASA Langley Research Center’s “Vehicle Analysis Analytics”, IBM Watson Content. Processed data becomes the corpus of information which is interrogated to provide examples of finding data for mass regression analysis, technology impacts on MPE (Mass Properties Engineering), mass properties control, standards, and other aspects of interest.

Cerro, Jeffrey A.↗

Knowledge, People, and Risk

NASA's mandate is to take risks to got into space while applying its best knowledge. NASA's knowledge is the result of scientific insights from research, engineering wisdom from experience, project management skills, safety and team consciousness and institutional support and collaboration. This presentation highlights NASA's organizational knowledge, communication and growth efforts.

Rogers, Edward W.↗

A Systems Architecting Methodology Using Bloom’s Taxonomy to Promote Creative Engineering Synthesis

Architecting complex systems requires high-level cognitive processing and extensive knowledge of the system elements, functions, relationships, and constraints. This paper describes a systems architecting methodology implemented through cognitive psychological creative processes using Bloom’s taxonomy as a framework to generate the expert knowledge required to effectively and systematically synthesize new systems. Systems architecting activities were carried out to identify, develop, and capture factual and conceptual knowledge relevant to the system subject matter and functional elements, as well as to facilitate active processing of the knowledge through remembering, understanding, applying, analyzing, evaluating, and creating. Dual channel and limited capacity principles of learning were incorporated into the format of the systems engineering tools developed to assist with information processing and retention. Meta-cognitive strategies associated with memory and creative idea generation were implemented into the methodology to effectively and efficiently develop system alternatives using the full collection of knowledge. Synthesis of an orbiting sample capture and orientation system architecture to enable spacecraft-based on orbit capture of a Mars sample container for a potential Mars Sample Return campaign was used as a case study.

Adajian, Rama↗

Post University On-the-Job Training for Engineers

Our national need for qualified scientists and engineers is greater now than at any other time in our history. Fortunately, we can point with pride to this need as a measure of the impact of science and technology on our way of life. In effect, we have made such rapid strides In advancing established sciences and in opening new technological fields that we have proved the value of the scientist and engineer to society, and, as a-result, have created an expanding demand for their services which we must now attempt to satisfy. This demand we face is also due to the changing skills and high degree of specialization required to perform in these new technological fields. The colleges and universities are doing their part to provide current graduates with a modern technical foundation, but we cannot afford to ignore the thousands of experienced engineers and scientists already employed by private industry and government. As employers, we have an obligation to these men and women to see that they are provided with an understanding of the latest advances that modern technology has to offer; that we develop them in particular specialty areas characteristic of a given field of work; and, equally important, that we assist them in the transition from one field to another as the technological emphasis shifts. Practically all technological industries have experienced and continue to experience rapid changes in their activities. The aerospace business, in particular, has been characterized by extremely rapid, in fact revolutionary, changes during the relatively short period of its existence0 At the National Aeronautics and Space Administration, successor to the National Advisory Committee for Aeronautics, for example, we have encountered the fun impact of a changing science and technology. Indeed, as a research organization, we have undoubtedly contributed, in some measure, to this change. Within the NASAs Lewis Research Center, we have approximately 800 research scientists and engineers who have matured professionally in an environment which is essentially one of continuous learning - an experience which comes close to being a form of post graduate training in itself. This environment, in addition to providing continuous evolutionary changes, has also provided two major revolutions which have made this development picture more complex. We will describe these environmental changes which have occurred at the Lewis Research Center and discuss the various techniques and programs we have employed to provide for the professional development of our staff. The Lewis Research Center has had an Interesting and exciting l8-year history of aerospace propulsion research and development. It began during the early years of World War II as an expansion of the Power Plant Division of the NCA Langley Center with the mission of conducting research required for the development of improved reciprocating engines and to study the associated problems of subsonic propulsion aerodynamics, It was only a few years later, however, that turbojet and ramjet propulsion and supersonic flight research became our main concern. This transition to jet type engines and higher speeds was our first major technological change. The aerodynamics of propellers became the aerodynamics of high speed turbine and compressor blades; the fuel ignition and carbon deposition problems were transferred from a cyclical or Intermittent high compression combustion chamber to a continuous combustion zone within a thin-walled metal shell; aerodynamics problems were thrust into the supersonic range; and high temperature materials began to play an increasingly critical role. Although this transition still required the same basic knowledge and principles as before, the new engine types did involve a different emphasis and variety of consideration not generally familiar to our scientists and engineers.

Manganiello, Eugene J.↗

Immersive Digital Twin Laboratory for Engineering Education (CRADA Final Report)

This project aimed to create an immersive digital twin laboratory that incorporates advanced tracking and visualization capabilities. In collaboration with Fort Lewis College, NREL designed a state-of-the-art physical visualization laboratory, developed a software platform to enable interaction with tracked physical objects in the laboratory, and provided proof-of-concept curricula that included manipulating these tracked objects. The project was initiated to address the growing need for innovative educational tools in engineering education. As renewable energy systems, particularly solar installations, become more complex, there is a pressing need to bridge the gap between theoretical knowledge and practical application. Traditional methods of teaching solar engineering concepts often fall short of providing students with a comprehensive, hands-on understanding. This immersive digital twin laboratory was conceived to fill that gap by creating a safe, non-energized setting where students can interact with augmented solar installation objects, gaining valuable insights into system performance, design, and maintenance. The project utilized extended reality (XR) technologies, including head-mounted displays (HMDs) and a whole-room optical motion tracking system, to connect physical objects with their digital twins in real time. The laboratory was equipped with MagicLeap 2 HMDs, supported by a Vicon Vero 2.2 Optical Tracking System, which provided precise 6-degrees-of-freedom (6-DOF) tracking. We developed a software platform to manage the interaction between the tracked physical objects and their virtual counterparts, enabling real-time data synchronization, object recognition, and virtual overlays. We designed the system to be flexible and extendable, allowing for future integration of additional objects and curriculum. This research advances the field of engineering education by demonstrating the potential of immersive digital twin environments. The laboratory provides a dynamic learning space where students can experiment, collaborate, and learn without the risks associated with live experimentation. The ability to simulate and manipulate solar installation objects under various conditions has broad implications for workforce development, particularly in renewable energy. The project also highlights the economic feasibility of using XR technologies in educational settings, offering a cost-effective solution for institutions looking to enhance their curriculum. By fostering a deeper understanding of solar energy systems, this work contributes to the broader goal of supporting the global energy transition and preparing the next generation of engineers and technicians.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Development and Implementation of a Small Satellite Systems Engineering Webinar Series: A Collaboration Between United Nations Office for Outer Space Affairs and National Aeronautics and Space Administration

The United Nations Office for Outer Space Affairs (UNOOSA) in collaboration with the National Aeronautics and Space Administration (NASA) established a webinar series on NASA systems engineering standards and practices for the purpose of sharing knowledge in this area through UNOOSA’s “Access to Space for All” which provides free and accessible educational content. Through NASA’s Small Spacecraft Systems Virtual Institute (S3VI), the four-part series was designed to cover basic systems engineering and project management skills that are fundamental for planning, developing, and implementing an experiment or a space project, and that serve as critical knowledge for those engaged in space activities, whether as a designer, builder, or manager of space infrastructure and services. This first series of webinars was convened over the course of November 2023 through February 2024. The webinar series was segmented into four one and a half hour webinars which covered topics related to the Fundamentals of Systems Engineering, Requirements and Systems Engineering, System Assembly, Integration and Test, and Spacecraft Handling, as well as an Introduction to the Small Spacecraft Systems Virtual Institute (S3VI). The agendas for the first three webinars focused on addressing a number of questions relevant to a particular topic. Examples of these questions include: What is systems engineering and what does a systems engineer do? Why does NASA use systems engineering? How do you define requirements for a small spacecraft mission? Why are requirements important to space missions? How are trade studies used to determine parts selection and why are they important? The final webinar of the first series provided interactive discussions and demonstrations of the S3VI tools; shared information on additional webinar opportunities offered by the S3VI; and reviewed databases curated by the S3VI that include the Small Satellite Reliability Initiative (SSRI) Knowledge Base Tool, Mission Design Tools, Small Spacecraft Information Search, and the State-of-the-Art (SoA) Small Spacecraft Report. Future webinar series will be conducted with topics are to be determined. The presenter will discuss details related to the first webinar series and future plans for additional series.

systems engineering↗

Significantly enhanced near-field coupling via tip engineering

The ability to significantly enhance near-field coupling between light and matter at the nanoscale is crucial for advancing the fields of nanophotonics and nanopolariotonics. However, conventional probes face challenges in achieving optimal light–matter interaction. In this study, we propose a novel, to the best of our knowledge, simulation-based strategy that leverages tip engineering to dramatically amplify the scattering field through tailored double-layer geometries. By employing a core-shell structure with a thin shell layer optimized for specific dielectric permittivity and effective polarizability, we demonstrate a near-field enhancement of up to 10 times compared to conventional probes. Our findings highlight exciting new possibilities for optimizing near-field interactions through probe designs with customized resonances, paving the way for substantially improved nano-optical sensing, imaging, and detection.

Shiravi, H.↗

A Comprehensive Analysis of Energy and Daylighting Impact of Window Shading Systems and Control Strategies on Commercial Buildings in the United States

Commercial buildings consume approximately 1.9 EJ of energy in the United States, 50% of which is for heating, cooling, and lighting applications. It is estimated that windows contribute up to 34% of the energy used for heating and cooling. However, window retrofits are not often undertaken to increase energy efficiency because of the high cost and disruptive nature of window installation. Highly efficient window technologies would also need shading devices for glare prevention and visual comfort. An automated window shading system with an appropriate control strategy is a technology that can reduce energy demand, maintain occupant comfort, and enhance the aesthetics and privacy of the built environment. However, the benefits of the automated shades currently used by the shading industry are not well studied. The topic merits an analysis that will help building owners, designers and engineers, and utilities make informed decisions using knowledge of the impact of this technology on energy consumption, peak demand, daylighting, and occupant comfort. This study uses integrated daylight and whole-building energy simulation to evaluate the performance of various control strategies that the shading industry uses in commercial office buildings. The analysis was performed for three different vintages of medium office buildings at six different locations in United States. The results obtained show the control strategies enabled cooling energy savings of up to 40% using exterior shading, and lighting energy savings of up to 25%. The control strategies described can help building engineers and researchers explore different control methods used to control shading in actual buildings but rarely discussed in the literature. This information will give researchers the opportunity to investigate potential improvements in current technologies and their performance.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Environmental Engineering for the 21st Century: Addressing Grand Challenges

Environmental engineers support the well-being of people and the planet in areas where the two intersect. Over the decades the field has improved countless lives through innovative systems for delivering water, treating waste, and preventing and remediating pollution in air, water, and soil. These achievements are a testament to the multidisciplinary, pragmatic, systems-oriented approach that characterizes environmental engineering. The future holds daunting challenges for human society and our environment. Populations are expanding, demand for resources is increasing, the climate is changing, and humanity’s impacts on the planet continue to mount. Will we be able to achieve a better quality of life for our growing population without compromising the ability of future generations to achieve the same? This study, authored by 18 of the nation’s leading environmental engineers, scientists, and policy experts under the auspices of the National Academies of Sciences, Engineering, and Medicine, outlines the crucial role for environmental engineers in this period of dramatic growth and change. The report identifies five pressing challenges of the 21st century that environmental engineers are uniquely poised to help advance: 1: Sustainably supply food, water, and energy, 2: Curb climate change and adapt to its impacts, 3: Design a future without pollution and waste, 4: Create efficient, healthy, resilient cities, and 5: Foster informed decisions and actions. The report’s vision is ambitious. The challenges ahead are substantial. Yet every day, environmental engineers are making progress, both by applying existing knowledge and skills and by advancing research and innovation to generate new insights and achievements. By refocusing and redoubling its efforts to advance practical, impactful solutions for humanity’s multifaceted, vexing problems, the field of environmental engineering can build on its past successes—and chart new territory—in the decades ahead.

42 ENGINEERING↗

Advanced Stirling Radioisotope Generator Engineering Unit 2 (ASRG EU2) Final Assembly

NASA Glenn Research Center (GRC) has recently completed the assembly of a unique Stirling generator test article for laboratory experimentation. Under the Advanced Stirling Radioisotope Generator (ASRG) flight development contract, NASA GRC initiated a task to design and fabricate a flight-like generator for in-house testing. This test article was given the name ASRG Engineering Unit 2 (EU2) as it was effectively the second engineering unit to be built within the ASRG project. The intent of the test article was to duplicate Lockheed Martin's qualification unit ASRG design as much as possible to enable system-level tests not previously possible at GRC. After the cancellation of the ASRG flight development project, the decision was made to continue the EU2 build, and make use of a portion of the hardware from the flight development project. GRC and Lockheed Martin engineers collaborated to develop assembly procedures, leveraging the valuable knowledge gathered by Lockheed Martin during the ASRG development contract. The ASRG EU2 was then assembled per these procedures at GRC with Lockheed Martin engineers on site. The assembly was completed in August 2014. This paper details the components that were used for the assembly, and the assembly process itself.

Stirling Cycle↗

The Use of Underground Research Laboratories to Support Repository Development Programs. A Roadmap for the Underground Research Facilities Network.

Under the auspices of the International Atomic Energy Agency (IAEA), nationally developed underground research laboratories (URLs) and associated research institutions are being offered for use by other nations. These facilities form an Underground Research Facilities (URF) Network for training in and demonstration of waste disposal technologies and the sharing of knowledge and experience related to geologic repository development, research, and engineering. In order to achieve its objectives, the URF Network regularly sponsors workshops and training events related to the knowledge base that is transferable between existing URL programs and to nations with an interest in developing a new URL. This report describes the role of URLs in the context of a general timeline for repository development. This description includes identification of key phases and activities that contribute to repository development as a repository program evolves from an early research and development phase to later phases such as construction, operations, and closure. This information is cast in the form of a matrix with the entries in this matrix forming the basis of the URF Network roadmap that will be used to identify and plan future workshops and training events.

MacKinnon, Robert J.↗

Use of generic and site-specific underground research laboratories to support siting, design, and safety assessment - 15417

Under the auspices of the International Atomic Energy Agency (IAEA), nationally developed underground research laboratories (URLs) and associated research institutions are being offered for use by other nations. These facilities form an Underground Research Facilities (URF) Network for training in and demonstration of waste disposal technologies and the sharing of knowledge and experience related to geologic repository development, research, and engineering. In order to achieve its objectives, the URF Network regularly sponsors workshops and training events related to the knowledge base that is transferable between existing URL programs and to nations with an interest in developing a new URL. Toward that end, a three-day workshop was held in Albuquerque, New Mexico in October 2014 to identify and compare the understanding of URF Network members on the place and role of generic and site-specific URLs in the overall, iterative geological disposal development process, including repository siting, design, and post-closure safety assessment. Attention was also given to the need for URLs in salt host rock, since there is currently no such facility in operation in the world. This paper describes some of the outcomes of the workshop in the context of the general timeline for repository development and the evolving role of research, development, and demonstration (RD and D) activities in URLs. Of particular interest is the relationship between technology maturity, as measured by technology readiness level (TRL), and the stages or milestones in the repository development timeline. The long history of RD and D in worldwide URLs (> 40 years) can be argued to be the primary reason that nuclear waste disposal systems are currently at a high level of maturity. One of the key recommendations from the workshop was that an international, easily accessible knowledge base should soon be established to avoid the loss of important experiences and information obtained from existing and closed URLs. It was also recognized that a new generic URL in salt host rock would provide a number of benefits, but one should not be initiated until a national program specifically decides to site its repository in salt. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

An Optimal Orthogonal Decomposition Method for Kalman Filter-Based Turbofan Engine Thrust Estimation

A new linear point design technique is presented for the determination of tuning parameters that enable the optimal estimation of unmeasured engine outputs such as thrust. The engine s performance is affected by its level of degradation, generally described in terms of unmeasurable health parameters related to each major engine component. Accurate thrust reconstruction depends upon knowledge of these health parameters, but there are usually too few sensors to be able to estimate their values. In this new technique, a set of tuning parameters is determined which accounts for degradation by representing the overall effect of the larger set of health parameters as closely as possible in a least squares sense. The technique takes advantage of the properties of the singular value decomposition of a matrix to generate a tuning parameter vector of low enough dimension that it can be estimated by a Kalman filter. A concise design procedure to generate a tuning vector that specifically takes into account the variables of interest is presented. An example demonstrates the tuning parameters ability to facilitate matching of both measured and unmeasured engine outputs, as well as state variables. Additional properties of the formulation are shown to lend themselves well to diagnostics.

Litt, Jonathan S.↗