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At least 811 records · Page 45

Optimal reference genes for RNA tissue analysis in small animal models of hemorrhagic fever viruses

Abstract Reverse-transcription quantitative polymerase chain reaction assays are frequently used to evaluate gene expression in animal model studies. Data analyses depend on normalization using a suitable reference gene (RG) to minimize effects of variation due to sample collection, sample processing, or experimental set-up. Here, we investigated the suitability of nine potential RGs in laboratory animals commonly used to study viral hemorrhagic fever infection. Using tissues (liver, spleen, gonad [ovary or testis], kidney, heart, lung, eye, brain, and blood) collected from naïve animals and those infected with Crimean–Congo hemorrhagic fever (mice), Nipah (hamsters), or Lassa (guinea pigs) viruses, optimal species-specific RGs were identified based on five web-based algorithms to assess RG stability. Notably, the Ppia RG demonstrated stability across all rodent tissues tested. Optimal RG pairs that include Ppia were determined for each rodent species ( Ppia and Gusb for mice; Ppia and Hrpt for hamsters; and Ppia and Gapdh for guinea pigs). These RG pair assays were multiplexed with viral targets to improve assay turnaround time and economize sample usage. Finally, a pan-rodent Ppia assay capable of detecting Ppia across multiple rodent species was developed and successfully used in ecological investigations of field-caught rodents, further supporting its pan-species utility.

Davies, Katherine A.↗

Prototype-Wise Sensitivity Analysis of Urban Building Energy Simulation Surrogate Modeling Accuracy

Urban Building Energy Modeling (UBEM) is an important reference for urban energy-related policymaking. Because of the significant impact of urban microclimates on the energy simulation, UBEM requires simulations of many microclimate-prototype pairs. Surrogate modeling is commonly used to reduce the cost of simulation computations. In UBEM surrogate modeling, it is important to determine the percentage of microclimates related to a prototype used for generating surrogate model training data. This study analyzes the prototype-wise variations and sensitivities of surrogate model estimation accuracy to the microclimate sampling ratios. The results of the study can help determine the number of simulations used for generating surrogate modeling data, avoid redundant simulations, and reduce the computational cost for UBEM surrogate modeling and its time.

Pan, Xiyu↗

Spectral Effects in Albedo and Rearside Irradiance Measurement for Bifacial Performance Estimation

We investigate the impact of spectral dependence of ground surface reflectivity on albedo and rearside irradiance measurements necessary for bifacial photovoltaic (PV) module performance estimation and monitoring. Because PV modules are spectrally selective, albedo and irradiance measurements performed with common irradiance sensors may require spectral mismatch corrections when used for performance prediction. We investigate via simulation the differences in spectrally responsive albedo measured with thermopile pyranometers and crystalline silicon PV reference cells in comparison to a typical crystalline-silicon bifacial PV module. Simulations are performed for nine different representative ground surface materials using simulated solar spectra together with spectral reflectivity data distributed with the SMARTS simulation software. For the materials considered, the results show that albedo spectral mismatch relative to the bifacial module is distributed over a range of ±9.2% for thermopile pyranometers versus only ±3.7% for a typical PV reference cell. We consider the impact of this spectrally-responsive albedo mismatch on bifacial PV module rearside irradiance measurements. Using synthesized rearside spectral irradiance distributions, we find that for the nine different ground surface materials the predicted rearside irradiance measurement deviates from the effective irradiance observed by the PV module by on the order of 16.5 W/m 2 for the pyranometer and 3.6 W/m 2 for the PV reference cell. We discuss the implications for bifacial albedo and irradiance measurement.

41 EE - Solar Energy Technologies Office (EE-4S)↗

Industrial Electrification Assessment Framework

Electrification refers to the process of transitioning from fuel-powered systems to electric-powered systems, or electrotechnologies. This document first provides an overview of industrial electrification, including possible advantages and common applications. Next, it provides a comprehensive, step-by-step framework for assessing the potential for electrification at an individual industrial facility. The steps in this framework include inventorying current fuel-powered systems, evaluating a manufacturing facility’s electrification readiness, and identifying, evaluating, and prioritizing strategies and technologies. This document also contains information about project implementation, and the appendices provide tools and resources to support organizations pursuing electrification.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Crystal Growth Using MEPHISTO

The shuttle flight experiment "In Situ Monitoring of Crystal Growth Using MEPHISTO" was accomplished during STS-87 as part of the fourth flight of the United States Microgravity Payload (USMP-4), which was flown from November 19 to December 5, 1997. The data returned from that flight are just now beginning to yield quantitative results. This project is an international collaboration: the furnace system known as MEPHISTO was built in France by CNES (French National Space Agency) and CEA (French Atomic Energy Commission); the principal investigator, Prof. Reza Abbaschian, is from the University of Florida at Gainesville; and numerical and analytical modeling support includes collaborators from the University of New South Wales, Australia, the University of Wisconsin at Milwaukee, the National Institute of Standards and Technology, and the NASA Lewis Research Center. MEPHISTO is a French acronym that translates into English as Materials for the Study of Interesting Phenomena of Solidification on Earth and in Orbit. Since this was the fourth flight of the MEPHISTO furnace, the experiment is referred to as MEPHISTO-4. MEPHISTO-4 was a directional solidification experiment that studied the liquid-to-solid transformation of bismuth alloyed with tin. Directional solidification is a freezing technique common to the processing of the electronic materials used in integrated circuits and detectors, such as silicon and germanium. When liquids are frozen on Earth, they must be cooled. The cooling causes stirring because of density variations in the liquid. This stirring, known as natural convection, influences the quality of the resulting solid. During freezing, regions of high and low concentrations of tin are created. This introduces another important phenomenon: diffusion, or the movement by molecular action of matter from regions of high concentration to regions of lower concentration. In MEPHISTO-4, it is tin that diffuses from the high-concentration region in front of the solid-liquid interface to more distant low-concentration regions.

deGroh, Henry C., III↗

Design of the Core Stage Inter-Tank Umbilical {CSITU) Compliance Mechanism

Project Goals: a) Design the compliance mechanism for the CSITU system to a 30% level -3D models completed in Pro/Engineer -Relevant design analysis b) Must meet all system requirements and establish basis for proceeding with detailed design. Tasks to be completed: A design that meets requirements for the 30% design review, 01/16/2013. Umbilical arms provide commodities to the launch vehicle prior to T-0. Commodities can range anywhere from hydraulics, pneumatics, cryogenic, electrical, ECS, etc ... Umbilicals commonly employ truss structures to deliver commodities to vehicle. Common configurations include: -Tilt-up -Swing Arm -Hose Drape -Drop Arm Umbilical arms will be mounted to Mobile Launch Platform. SLS currently has 9 T-0 umbilical arms. The compliance refers to the ability of the umbilical to adjust to minor changes in vehicle location. The compliance mechanism refers to the mechanism on the ground support equipment {GSE) that compensates for these changes. For the CSITU, these minor changes, or vehicle excursions, can be up to +4 in. Excursions refer to movements of the vehicle caused by wind loads and thermal expansion. It is ideal to have significant vertical compliance so a passive secondary release mechanism may be implemented.

Smith, Kurt R.↗

Mars Sample Return and Flight Test of a Small Bimodal Nuclear Rocket and ISRU Plant

A combined Nuclear Thermal Rocket (NTR) flight test and Mars Sample Return mission (MSR) is explored as a means of "jump-starting" NTR development. Development of a small-scale engine with relevant fuel and performance could more affordably and quickly "pathfind" the way to larger scale engines. A flight test with subsequent inflight postirradiation evaluation may also be more affordable and expedient compared to ground testing and associated facilities and approvals. Mission trades and a reference scenario based upon a single expendable launch vehicle (ELV) are discussed. A novel "single stack" spacecraft/lander/ascent vehicle concept is described configured around a "top-mounted" downward firing NTR, reusable common tank, and "bottom-mount" bus, payload and landing gear. Requirements for a hypothetical NTR engine are described that would be capable of direct thermal propulsion with either hydrogen or methane propellant, and modest electrical power generation during cruise and Mars surface insitu resource utilization (ISRU) propellant production.

George, Jeffrey A.↗

It's All Relative: A Validation of Radiation Quality Comparison Metrics

Historically, the relative biological effectiveness (RBE) has been calculated to quantify the difference between heavy ion and gamma ray radiation. The RBE is then applied to gamma ray data to predict the effects of heavy ions in humans. The RBE is an iso-effect dose-to-dose ratio which, due to its counterintuitive nature, has been commonly miscalculated as an iso-dose effect-to-effect ratio. A paper recently published by Shuryak et al described this second measure intentionally for the first time in 2017, referring to it as the radiation effects ratio (RER). In this study, we utilized simulations to test the ability of both the RBE and the RER to predict known heavy ion effects. RBEs and RERs were calculated using mouse data from Chang et al, and the ability of the RBE and RER to predict the heavy ion data from which they were calculated was verified. Statistical transformations often utilized during data analysis were applied to the gamma and heavy ion data to determine whether RBE and RER are each uniquely defined measures. Scale changes are expected when translating effects from mice to humans and between human populations; gamma and heavy ion data were transformed to represent potential scale changes. The ability of the RBE and RER to predict the transformed heavy ion data from the transformed gamma data was then tested. The RBE but not the RER was uniquely defined after all statistical transformations. The RBE correctly predicted the scale-transformed heavy ion data, while the RER did not. This presentation describes potential implications for both metrics in light of these findings.

Chappell, L. J.↗

International Docking System Standard (IDSS) Interface Definition Document (IDD) Revision F

This IDSS IDD details the physical geometric mating interface and design loads requirements. The physical geometric interface requirements must be strictly followed to ensure physical spacecraft mating compatibility. This includes both defined components and areas that are void of components. The IDD also identifies common design parameters as identified in Section 3.0, e.g., docking initial conditions and vehicle mass properties. This information represents a recommended set of design values enveloping a broad set of design reference missions and conditions, which if accommodated in the docking system design, increases the probability of successful docking between different spacecraft.

Stanley R Donahoe↗

Utilization of Streamtubes to Analyze the Physical Interaction of a Dispersed Cloud with the CRM65 Hybrid Midspan Model

Recently there have been numerous efforts to identify the relevant icing physics related to the formation of complex three dimensional features, sometimes referred to as ’scallops’, on swept wings. However, much of the physics is still not well understood. This paper computationally investigates the interaction of the icing cloud with the 65 percent Common Research Model (CRM65). Both the interaction with an uniced model, and the interaction with a representative simulated three dimensional ice accretion are analyzed. Preliminary results suggest that the liquid water content increases near the aerodynamic body. For small droplets on the uniced geometry, the particle velocity vector becomes nearly parallel with the aerodynamic model resulting in a low value of collection efficiency. When a three dimensional feature with a length scale much smaller than the leading edge of the airfoil is introduced into the flow, the impingement of small particles can become significantly more perpendicular to the particle velocity vector in the region of these features. Since the liquid water content near the body has increased due to the interaction with the larger scale features of the aerodynamic model, i.e. the leading edge of the swept wing, extremely high collection efficiency is observed. These results suggest that three dimensional features are likely a significant physical driver that should be modeled in some capacity when simulating the impingement of a cloud on an aerodynamic model.

Icing↗

Utilization of Streamtubes to Analyze the Physical Interaction of a Dispersed Cloud with the CRM65 Hybrid Midspan Model

Recently there have been numerous efforts to identify the relevant icing physics related to the formation of complex three dimensional features, sometimes referred to as ’scallops’, on swept wings. However, much of the physics is still not well understood. This paper computationally investigates the interaction of the icing cloud with the 65 percent Common Research Model (CRM65). Both the interaction with an uniced model, and the interaction with a representative simulated three dimensional ice accretion are analyzed. Preliminary results suggest that the liquid water content increases near the aerodynamic body. For small droplets on the uniced geometry, the particle velocity vector becomes nearly parallel with the aerodynamic model resulting in a low value of collection efficiency. When a three dimensional feature with a length scale much smaller than the leading edge of the airfoil is introduced into the flow, the impingement of small particles can become significantly more perpendicular to the particle velocity vector in the region of these features. Since the liquid water content near the body has increased due to the interaction with the larger scale features of the aerodynamic model, i.e. the leading edge of the swept wing, extremely high collection efficiency is observed. These results suggest that three dimensional features are likely a significant physical driver that should be modeled in some capacity when simulating the impingement of a cloud on an aerodynamic model.

Icing↗

Bayesian reduced-order deep learning surrogate model for dynamic systems described by partial differential equations

We propose a reduced-order deep-learning surrogate model for dynamic systems described by time-dependent partial differential equations. This method employs space–time Karhunen–Loève expansions (KLEs) of the state variables and space-dependent KLEs of space-varying parameters to identify the reduced (latent) dimensions. Subsequently, a deep neural network (DNN) is used to map the parameter latent space to the state variable latent space. An approximate Bayesian method is developed for uncertainty quantification (UQ) in the proposed KL-DNN surrogate model. The KL-DNN method is tested for the linear advection–diffusion and nonlinear diffusion equations, and the Bayesian approach for UQ is compared with the deep ensembling (DE) approach, commonly used for quantifying uncertainty in DNN models. It was found that the approximate Bayesian method provides a more informative distribution of the PDE solutions in terms of the coverage of the reference PDE solutions (the percentage of nodes where the reference solution is within the confidence interval predicted by the UQ methods) and log predictive probability. The DE method is found to underestimate uncertainty and introduce bias. For the nonlinear diffusion equation, we compare the KL-DNN method with the Fourier Neural Operator (FNO) method and find that KL-DNN is 10% more accurate and needs less training time than the FNO method.

97 MATHEMATICS AND COMPUTING↗

Determining the tilt of the Raman laser beam using an optical method for atom gravimeters

The tilt of a Raman laser beam is a major systematic error in precision gravity measurement using atom interferometry. The conventional approach to evaluating this tilt error involves modulating the direction of the Raman laser beam and conducting time-consuming gravity measurements to identify the error minimum. In this work, we demonstrate a method to expediently determine the tilt of the Raman laser beam by transforming the tilt angle measurement into characterization of parallelism, which integrates the optical method of aligning the laser direction, commonly used in freely falling corner-cube gravimeters, into an atom gravimeter. A position-sensing detector (PSD) is utilized to quantitatively characterize the parallelism between the test beam and the reference beam, thus measuring the tilt precisely and rapidly. After carefully positioning the PSD and calibrating the relationship between the distance measured by the PSD and the tilt angle measured by the tiltmeter, we achieved a statistical uncertainty of less than 30 µrad in the tilt measurement. Furthermore, we compared the results obtained through this optical method with those from the conventional tilt modulation method for gravity measurement. The comparison validates that our optical method can achieve tilt determination with an accuracy level of better than 200 µrad, corresponding to a systematic error of 20 µGal in g measurement. This work has practical implications for real-world applications of atom gravimeters.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Core Physics Characteristics of Extended Enrichment and High Burnup Boiling Water Reactor Fuel

This paper presents the highlights of boiling water reactor (BWR) core physics studies performed at Oak Ridge National Laboratory as part of a series of studies conducted to compare low-enriched uranium (LEU) with LEU+ fuel. The studies analyzed isotopic fuel content, lattice parameters (Phase 1), and core physics (Phase 2) to identify challenges in operation, storage, and transportation for BWRs and pressurized water reactors (PWRs). Because of a lack of publicly available lattice and core designs for modern BWR fuel assemblies and reactor cores, several optimized lattice designs were generated, and different core loading strategies were investigated. Twelve optimized lattice designs with 235 U enrichments ranging from 1.6% to 9% and gadolinia loadings ranging from 3 to 8 wt% were used to model axial enrichment and geometry variations in fuel assemblies for core designs. Each core shares a common set of approximations in design and analysis to allow for consistent comparisons between LEU and LEU+ fuel. The objective is to highlight anticipated changes in core behavior with respect to the reference LEU core. The results of this study show that the differences in LEU and LEU+ core reactor physics characteristics are less significant than the differences in lattice physics characteristics reported in the Phase 1 studies.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Information services for comparative analysis of biorhythm research

References and full text documents are presented in support of continuing research and research planning for the NASA behavioral physiology program. Areas covered include: (1) desynchronosis and performance; (2) effects of alcohol, common colds, drugs, and toxic hazards on performance; (3) effects of stress on rhythm of plasma steroids; (4) data processing of biological rhythms; (5) pharmacology and biological rhythms; (6) mechanisms of biological rhythms; and (7) development of biological rhythms.

Source record↗

Use of human engineering standards in design

Results are presented for a research study intended to assess the impact of present human engineering standards on product design. The approach consisted of three basic steps: a comparison of two display panels to determine if, in fact, products designed to the same standards are truly standardized; a review of two existing standards to determine how well their information can be used to solve design problems; and a survey of human factors specialists to assess their opinions about standards. It is shown that standards have less than the desired influence on product design. This is evidenced by a lack of standardization between hardware designed under common standards, by deficiencies within the standards that detract from their usefulness and encourage users to ignore them, and by the respondents of the survey who consider standards less valuable than other reference sources for design implementation. Recommendations aimed at enhancing the use of standards are set forth.

Rogers, J. G.↗

Space station integrated propulsion and fluid systems study. Space station program fluid management systems databook

Commonality and integration of propulsion and fluid systems associated with the Space Station elements are being evaluated. The Space Station elements consist of the core station, which includes habitation and laboratory modules, nodes, airlocks, and trusswork; and associated vehicles, platforms, experiments, and payloads. The program is being performed as two discrete tasks. Task 1 investigated the components of the Space Station architecture to determine the feasibility and practicality of commonality and integration among the various propulsion elements. This task was completed. Task 2 is examining integration and commonality among fluid systems which were identified by the Phase B Space Station contractors as being part of the initial operating capability (IOC) and growth Space Station architectures. Requirements and descriptions for reference fluid systems were compiled from Space Station documentation and other sources. The fluid systems being examined are: an experiment gas supply system, an oxygen/hydrogen supply system, an integrated water system, the integrated nitrogen system, and the integrated waste fluids system. Definitions and descriptions of alternate systems were developed, along with analyses and discussions of their benefits and detriments. This databook includes fluid systems descriptions, requirements, schematic diagrams, component lists, and discussions of the fluid systems. In addition, cost comparison are used in some cases to determine the optimum system for a specific task.

Bicknell, B.↗

Compendium of Information for Interpreting the Microgravity Environment of the Orbiter Spacecraft

Science experiments are routinely conducted on the NASA shuttle orbiter vehicles. Primarily, these experiments are operated on such missions to take advantage of the microgravity (low-level acceleration) environment conditions during on-orbit operations. Supporting accelerometer instruments are operated with the experiments to measure the microgravity acceleration environment in which the science experiments were operated. Tne Principal Investigator Microgravity Services (PIMS) Project at NASA Lewis Research Center interprets these microgravity acceleration data and prepares mission summary reports to aid the principal investigators of the scientific experiments in understanding the microgravity environment. Much of the information about the orbiter vehicle and the microgravity environment remains the same for each mission. Rather than repeat that information in each mission summary report, reference information is presented in this report to assist users in understanding the microgravity-acceleration data. The characteristics of the microgravity acceleration environment are first presented. The methods of measurement and common instruments used on orbiter missions are described. The coordinate systems utilized in the orbiter and accelerometers are described. Some of the orbiter attitudes utilized in microgravity related missions are illustrated. Methods of data processing are described and illustrated. The interpretation of the microgravity acceleration data is included with an explanation of common disturbance sources. Instructions to access some of the acceleration data and a description of the orbiter thrusters are explained in the appendixes. A microgravity environment bibliography is also included.

DeLombard, Richard↗