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At least 811 records · Page 45

Mutagenesis in human cells with accelerated H and Fe ions

The overall goals of this research were to determine the risks of mutation induction and the spectra of mutations induced by energetic protons and iron ions at two loci in human lymphoid cells. During the three year grant period the research has focused in three major areas: (1) the acquisition of sufficient statistics for human TK6 cell mutation experiments using Fe ions (400 MeV/amu), Fe ions (600 MeV/amu) and protons (250 MeV/amu); (2) collection of thymidine kinase- deficient (tk) mutants or hypoxanthine phosphoribosyltransferase deficient (hprt) mutants induced by either Fe 400 MeV/amu, Fe 600 MeV/amu, or H 250 MeV/amu for subsequent molecular analysis; and (3) molecular characterization of mutants isolated after exposure to Fe ions (600 MeV/amu). As a result of the shutdown of the BEVALAC heavy ion accelerator in December 1992, efforts were rearranged somewhat in time to complete our dose-response studies and to complete mutant collections in particular for the Fe ion beams prior to the shutdown. These goals have been achieved. A major effort was placed on collection, re-screening, and archiving of 3 different series of mutants for the various particle beam exposures: tk-ng mutants, tk-sg mutants, and hprt-deficient mutants. Where possible, groups of mutants were isolated for several particle fluences. Comparative analysis of mutation spectra has occured with characterization of the mutation spectrum for hprt-deficient mutants obtained after exposure of TK6 cells to Fe ions (600 MeV/amu) and a series of spontaneous mutants.

Kronenberg, Amy↗

Grazing-Activated Production of Dimethyl Sulfide (DMS) by two clones of Emiliania huxleyi

Emiliania huxleyi clones CCMP 370 and CCMP 373 produced similar amounts of dimethylsulfoniopropionate (DMSP) during axenic exponential growth, averaging 109 mM internal DMSP. Both clones had detectable DMSP lyase activity, as measured by production of dimethyl sulfide (DMS) during in vitro assays of crude cell preparations, but activities and conditions differed considerably between clones. Clone 373 had high activity; clone 370 had low activity and required chloride. For both strains, enzyme activity per cell was constant during exponential growth, but little DMS was produced by healthy cells. Rather, DMS production was activated when cells were subjected to physical or chemical stresses that caused cell lysis. We propose that DMSP lyase and DMSP are segregated within these cells and re-action only under conditions that result in cell stress or damage. Such activation occurs during microzooplankton grazing. When these clones were grazed by the dinoflagellate Oxyrrhis marina, DMS was produced; ungrazed cells, as well as those exposed to grazer exudates and associated bacteria, generated no DMS. Grazing of clone 373 produced much more DMS than grazing of clone 370, consistent with their relative in vitro DMSP lyase activities. DMS was only generated when cells were actually being grazed, indicating that ingested cells were responsible for the DMS formation. We suggest that even low levels of grazing can greatly accelerate DMS production.

Wolfe, Gordon V.↗

The Influence of Passive Acceleration and Exercise+Acceleration on Work Capacity and Orthostasis

The losses of aerobic power and orthostatic tolerance are significant effects of manned C) spaceflight that can negatively impact crew health and safety. Daily acceleration and aerobic training may ameliorate these effects. To determine the influence of passive intermittent +Gz acceleration (PA) training and active acceleration + interval exercise (AE) training on work 0 0 capacity and the acute (1 min) response to 70 deg head-up tilt, 6 men (X-Bar SD: age, 33 +/- 6 y; height, 178.3 +/- 4.6 cm; mass, 86.3 +/- 6.6 kg) participated in two 3-wk training protocols. It was hypothesized that PA and AE training would improve orthostatic tolerance and that the addition of aerobic conditioning, would not alter this effect.

Simonson, S. R.↗

Validation of Finite Element Crash Test Dummy Models for Predicting Orion Crew Member Injuries During a Simulated Vehicle Landing

A series of crash tests were conducted with dummies during simulated Orion crew module landings at the Wright-Patterson Air Force Base. These tests consisted of several crew configurations with and without astronaut suits. Some test results were collected and are presented. In addition, finite element models of the tests were developed and are presented. The finite element models were validated using the experimental data, and the test responses were compared with the computed results. Occupant crash data, such as forces, moments, and accelerations, were collected from the simulations and compared with injury criteria to assess occupant survivability and injury. Some of the injury criteria published in the literature is summarized for completeness. These criteria were used to determine potential injury during crew impact events.

Tabiei, Al↗

Nanoscale Metal Oxide Semiconductors for Gas Sensing

A report describes the fabrication and testing of nanoscale metal oxide semiconductors (MOSs) for gas and chemical sensing. This document examines the relationship between processing approaches and resulting sensor behavior. This is a core question related to a range of applications of nanotechnology and a number of different synthesis methods are discussed: thermal evaporation- condensation (TEC), controlled oxidation, and electrospinning. Advantages and limitations of each technique are listed, providing a processing overview to developers of nanotechnology- based systems. The results of a significant amount of testing and comparison are also described. A comparison is made between SnO2, ZnO, and TiO2 single-crystal nanowires and SnO2 polycrystalline nanofibers for gas sensing. The TECsynthesized single-crystal nanowires offer uniform crystal surfaces, resistance to sintering, and their synthesis may be done apart from the substrate. The TECproduced nanowire response is very low, even at the operating temperature of 200 C. In contrast, the electrospun polycrystalline nanofiber response is high, suggesting that junction potentials are superior to a continuous surface depletion layer as a transduction mechanism for chemisorption. Using a catalyst deposited upon the surface in the form of nanoparticles yields dramatic gains in sensitivity for both nanostructured, one-dimensional forms. For the nanowire materials, the response magnitude and response rate uniformly increase with increasing operating temperature. Such changes are interpreted in terms of accelerated surface diffusional processes, yielding greater access to chemisorbed oxygen species and faster dissociative chemisorption, respectively. Regardless of operating temperature, sensitivity of the nanofibers is a factor of 10 to 100 greater than that of nanowires with the same catalyst for the same test condition. In summary, nanostructure appears critical to governing the reactivity, as measured by electrical resistance of these SnO2 nanomaterials towards reducing gases. With regard to the sensitivity of the different nascent nanostructures, the electrospun nanofibers appear preferable

Hunter, Gary W.↗

An Alternative for Emergency Preemption of Traffic Lights

An electronic communication-and-control system has been developed as a prototype of advanced means of automatically modifying the switching of traffic lights to give priority to emergency vehicles. This system could be used alternatively or in addition to other emergency traffic-light-preemption systems, including a variety of systems now in use as well as two proposed systems described in "Systems Would Preempt Traffic Lights for Emergency Vehicles" (NPO-30573), NASA Tech Briefs, Vol. 28, No. 10 (October 2004), page 36. Unlike those prior systems that depend on detection of sounds and/or lights emitted by emergency vehicles, this system is not subject to severe range limitations. This system can be retrofitted into any pre-existing traffic-light-control system, without need to modify that system other than to make a minimal number of wire connections between the two systems. This system comprises several subsystems, including a transponder and interface circuitry on each emergency vehicle, a monitoring and control unit at each intersection equipped with traffic lights, and a wide-area two-way radio communication network that connects the emergency vehicles and intersection units. Computers in the various intersections and vehicle units run special-purpose software that implements the traffic- light-preemption scheme. The operations of the intersection and vehicle units are synchronized by use of Global Positioning System (GPS) timing signals. The transponder in each vehicle estimates its own position and velocity by use of GPS signals, deductive ("dead") reckoning, data from the onboard diagnostic (OBD) computer of the vehicle, and/or triangulation of beacon signals. When the operator of an emergency vehicle turns on its flashing lights and sirens in response to a request for an emergency response, the transponder unit goes into action, reading the OBD data to determine speed and acceleration, and reading and gathering further navigational data as described above. The position, velocity, and acceleration data are combined with vehicle-identification data in a prescribed format, and the resulting set of data is transmitted to the intersections within communication range of the transponder.

Foster, Conrad↗

Global Climate Impacts of Greenland and Antarctic Meltwater: A Comparative Study

Both the Greenland and Antarctic ice sheets have been melting at an accelerating rate over recent decades. Meltwater from Greenland might be expected to initiate a climate response which is distinct, and perhaps different from, that associated with Antarctic meltwater. Which one might elicit a greater climate response, and what mechanisms are involved? To explore these questions, we apply “Climate Response Functions (CRFs)” to guide a series of meltwater perturbation experiments using a fully-coupled climate model. In both hemispheres, meltwater drives atmospheric cooling, sea-ice expansion, and strengthened Hadley and Ferrel cells. Greenland meltwater induces a slowdown of the Atlantic Meridional Overturning Circulation (AMOC) and a cooling of the subsurface ocean in the northern high-latitudes. Antarctic meltwater, instead, induces a slowdown of the Antarctic Bottom Water formation and a warming of the subsurface ocean around Antarctica. For melt-rates up to 2000 Gt yr −1 , the climate response is rather linear. However, as melt-rates increase to 5000 Gt yr −1 , the climate response becomes non-linear. Due to a collapsed AMOC, the climate response is super-linear at high Greenland melt-rates. Instead, the climate response is sub-linear at high Antarctic melt-rates, due to the halting of the northward expansion of Antarctic sea ice by warm surface waters. Finally, in the linear limit, we use CRFs and linear convolution theory to make projections of important climate parameters in response to meltwater scenarios, which suggest that Antarctic meltwater will become a major driver of climate change, dominating that of Greenland meltwater, as the current century proceeds.

Greenland ice sheet↗

A proposal to determine properties of the gravitropic response of plants in the absence of a complicating g-force (GTHRES)

Gravitropic responses of oat seedlings (Avena sativa L.) were measured on Earth and in microgravity (IML-1). The seedlings were grown at 1 g either on Earth or on 1 g centrifuges. They were challenged by centripetal accelerations for which the intensity and duration of the stimulations were varied. All stimulation intensities were in the hypogravity region from 0.1 to 1.0 g. All responses occurred either in Spacelab microgravity or during clinorotation on Earth. The experiments were carried out with the same apparatus in Spacelab and on Earth. The experiments addressed a series of scientific questions and useful data were obtained to provide answers to some but not all of those questions.

Brown, Allan H.↗

Experimental studies for determining human discomfort response to vertical sinusoidal vibration

A study was conducted to investigate several problems related to methodology and design of experiments to obtain human comfort response to vertical sinusoidal vibration. Specifically, the studies were directed to the determination of (1) the adequacy of frequency averaging of vibration data to obtain discomfort predictors, (2) the effect of practice on subject ratings, (3) the effect of the demographic factors of age, sex, and weight, and (4) the relative importance of seat and floor vibrations in the determination of measurement and criteria specification location. Results indicate that accurate prediction of discomfort requires knowledge of both the acceleration level and frequency content of the vibration stimuli. More importantly, the prediction of discomfort was shown to be equally good based upon either floor accelerations or seat accelerations. Furthermore, it was demonstrated that the discomfort levels in different seats resulting from similar vibratory imputs were equal. Therefore, it was recommended that criteria specifications and acceleration measurements be made at the floor location. The results also indicated that practice did not systematically influence discomfort responses nor did the demographic factors of age, weight, and sex contribute to the discomfort response variation.

Dempsey, T. K.↗

Accelerance Decoupling: An Approach for Removing the Influence of the Test Stand from the Integrated Modal Test

The main objective for launch vehicle (LV) modal testing is to quantify the LV’s modal properties in the free-free state (post pad separation). However, given the size of most LV systems, free-free testing is a challenge and often not feasible. With this, a test stand, typically the launch pad itself, is introduced as the means of support. This shifts the challenge to developing robust numerical methods for removing the influence of the launch pad from the integrated system modal test. The Space Launch System (SLS) is no exception where the mobile launcher (ML) is used to support the vehicle for the integrated modal test (IMT). For the IMT, it is well understood from pre-test analysis with finite element models (FEMs) of the SLS and SLS coupled to ML that the ML has a significant influence on the SLS modal properties especially in the lower frequency range where the primary SLS bending modes exist. An accelerance decoupling (AD) method has been formulated for the purpose of “subtracting out” the influence of the ML from the IMT results. With AD, the SLS decoupled frequency response functions (FRFs) are directly extracted from the IMT FRFs. The subject approach is aimed to utilize measured data only and achieve a robust FRF decoupling scheme. AD is derived from a widely used coupling technique called “Receptance Coupling” (RC). The AD core equation reverses the RC process and utilizes a pair of auxiliary equations that enable the core equation to be resolved based on measured data only. In AD, the decoupled component FRFs are extracted from the coupled system FRFs with a transformation to remove the contribution of the “subtractive component”. This paper addresses the AD’s operational flexibility to resolve SLS free-free modal properties from coupled system measured data but also the possibility to include data from FEM if there is enough confidence in the FEM or if it is asserted that the effect to the final outcome is reasonable.

Accelerance decoupling↗

Accelerance Decoupling: An Approach for Removing the Influence of the Test Stand from the Integrated Modal Test

The main objective for launch vehicle (LV) modal testing is to quantify the LV’s modal properties in the free-free state (post pad separation). However, given the size of most LV systems, free-free testing is a challenge and often not feasible. With this, a test stand, typically the launch pad itself, is introduced as the means of support. This shifts the challenge to developing robust numerical methods for removing the influence of the launch pad from the integrated system modal test. The Space Launch System (SLS) is no exception where the mobile launcher (ML) is used to support the vehicle for the integrated modal test (IMT). For the IMT, it is well understood from pre-test analysis with finite element models (FEMs) of the SLS and SLS coupled to ML that the ML has a significant influence on the SLS modal properties especially in the lower frequency range where the primary SLS bending modes exist. An accelerance decoupling (AD) method has been formulated for the purpose of “subtracting out” the influence of the ML from the IMT results. With AD, the SLS decoupled frequency response functions (FRFs) are directly extracted from the IMT FRFs. The subject approach is aimed to utilize measured data only and achieve a robust FRF decoupling scheme. AD is derived from a widely used coupling technique called “Receptance Coupling” (RC). The AD core equation reverses the RC process and utilizes a pair of auxiliary equations that enable the core equation to be resolved based on measured data only. In AD, the decoupled component FRFs are extracted from the coupled system FRFs with a transformation to remove the contribution of the “subtractive component”. This paper addresses the AD’s operational flexibility to resolve SLS free-free modal properties from coupled system measured data but also the possibility to include data from FEM if there is enough confidence in the FEM or if it is asserted that the effect to the final outcome is reasonable.

Accelerance decoupling↗

Harmonic response of cylindrical and toroidal shells to an internal acoustic field. I - Theory. II - Results

In the first part of the present work concerning the coupled elastic and acoustic response of a system of cylindrical and toroidal shells enclosing an acoustic medium, the theoretical model for the cylindrical and toroidal segments' elastodynamics incorporates an elastic simulation based on transfer matrices, while the acoustic simulation adapts Green's function and curved surface elements. The coupled response is determined by the equilibrium of the acoustic pressure and internal elastic reactions of the shell, and compatibility between acoustic and elastic accelerations at the shell-fluid interface. The second part of this work obtains and discusses numerical results based on the theory for the cases of shell configurations having a plane of symmetry, as well as asymmetric ones. The inadequacy of beam theory in modeling the response of short, thin shell configurations at frequencies above the fundamental elastic resonance is demonstrated.

El-Raheb, M.↗

Accelerance Decoupling: An Approach for Removing the Influence of the Test Stand from the Integrated Modal Test

The main objective for launch vehicle (LV) modal testing is to quantify the LV’s modal properties in the free-free state (post pad separation). However, given the size of most LV systems, free-free testing is a challenge and often not feasible. With this, a test stand, typically the launch pad itself, is introduced as the means of support. This shifts the challenge to developing robust numerical methods for removing the influence of the launch pad from the integrated system modal test. The Space Launch System (SLS) is no exception where the mobile launcher (ML) is used to support the vehicle for the integrated modal test (IMT). For the IMT, it is well understood from pre-test analysis with finite element models (FEMs) of the SLS and SLS coupled to ML that the ML has a significant influence on the SLS modal properties especially in the lower frequency range where the primary SLS bending modes exist. An accelerance decoupling (AD) method has been formulated for the purpose of “subtracting out” the influence of the ML from the IMT results. With AD, the SLS decoupled frequency response functions (FRFs) are directly extracted from the IMT FRFs. The subject approach is aimed to utilize measured data only and achieve a robust FRF decoupling scheme. AD is derived from a widely used coupling technique called “Receptance Coupling” (RC). The AD core equation reverses the RC process and utilizes a pair of auxiliary equations that enable the core equation to be resolved based on measured data only. In AD, the decoupled component FRFs are extracted from the coupled system FRFs with a transformation to remove the contribution of the “subtractive component”. This paper addresses the AD’s operational flexibility to resolve SLS free-free modal properties from coupled system measured data but also the possibility to include data from FEM if there is enough confidence in the FEM or if it is asserted that the effect to the final outcome is reasonable.

Accelerance decoupling↗

Comparative transduction mechanisms of hair cells in the bullfrog utriculus. 1: Responses to intracellular current

Hair cells in the bullfrog sacculus are specifically adapted to sense small-amplitude, high-frequency linear accelerations. These hair cells display many properties that are undesirable or inappropriate for hair cells that must provide static gravity sensitivity. This study resulted in part due to an interest in seeing how the transduction mechanisms of hair cells in a gravity-sensing otolith endorgan would differ from those in the bullfrog sacculus. The bullfrog utriculus is an appropriate model for these studies, because its structure is representative of higher vertebrates in general and its function as a sensor of static gravity and dynamic linear acceleration is well known. Hair cells in the bullfrog utriculus, classifiable as Type 2 by cell body and synapse morphology, differ markedly in hair bundle morphology from those in the bullfrog sacculus. Moreover, the hair bundle morphologies of utricular hair cells, unlike those in the sacculus, differ in different membrane regions.

Baird, Richard A.↗

An evaluation of higher-order model methods for calculating transient structural response

A higher-order modal method proposed by Leung for transient structural analysis entitled the force-derivative method is evaluated. This method repeatedly integrates by parts with respect to time the convolution-integral form of the structural response to produce successively better approximations to the contribution of the higher modes which are neglected in the modal summation. Comparisons are made of the force-derivative, the mode-displacement, and the mode-acceleration methods for several numerical example problems for various times, levels of damping, and forcing functions. The example problems include a tip-loaded cantilevered beam and a simply-supported multispan beam. The force-derivative method is shown to converge to an accurate solution in fewer modes than either the mode-displacement or the mode-acceleration methods. In addition, for problems in which there are a large number of closely-spaced frequencies whose mode shapes have a negligible contribution to the response, the force-derivative method is very effective in representing the effect of the important, but otherwise neglected, higher modes.

Camarda, Charles J.↗

An evaluation of higher-order modal methods for calculating transient structural response

The present study evaluates a higher-order modal method proposed by Leung for transient structural analysis entitled the force-derivative method. This method repeatedly integrates by parts with respect to time the convolution-integral form of the structural response to produce successively better approximations to the contribution of the higher modes which are neglected in the modal summation. Comparisons are made of the force-derivative, the mode-displacement, and the mode-acceleration methods for several numerical example problems for various times, levels of damping, and forcing functions. The example problems include a tip-loaded cantilevered beam and a simply-supported multispan beam. The force-derivative method is shown to converge to an accurate solution in fewer modes than either the mode-displacement or the mode-acceleration methods. In addition, for problems in which there are a large number of closely-spaced frequencies whose mode shapes have a negligible contribution to the response, the force derivative method is very effective in representing the effect of the important, but otherwise neglected, higher modes.

Camarda, Charles J.↗

Synthetic Absorption Lines for a Clumpy Medium: A Spectral Signature for Cloud Acceleration in AGN?

There is increasing evidence that the highly ionized multiphase components of AGN disc winds may be due to thermal instability. The ions responsible for forming the observed X-ray absorption lines may only exist in relatively cool clumps that can be identified with the so-called warm absorbers. Here we calculate synthetic absorption lines for such warm absorbers from first principles by combining 2D hydrodynamic solutions of a two-phase medium with a dense grid of photoionization models to determine the detailed ionization structure of the gas. Our calculations reveal that cloud disruption, which leads to a highly complicated velocity field (i.e. a clumpy flow), will only mildly affect line shapes and strengths when the warm gas becomes highly mixed but not depleted. Prior to complete disruption, clouds that are optically thin to the driving UV resonance lines will cause absorption at an increasingly blueshifted line-of-sight velocity as they are accelerated. This behavior will imprint an identifiable signature on the line profile if warm absorbers are enshrouded in an even broader absorption line produced by a high column of intercloud gas. Interestingly, we show that it is possible to develop a spectral diagnostic for cloud acceleration by differencing the absorption components of a doublet line, a result that can be qualitatively understood using a simple partial covering model. Our calculations also permit us to comment on the spectral differences between cloud disruption and ionization changes driven by flux variability. Notably, cloud disruption offers another possibility for explaining absorption line variability.

line: formation – galaxies: nuclei – quasars: ↗

Remotely Sensed Northern Vegetation Response to Changing Climate: Growing Season and Productivity Perspective

Vegetation growing season and maximum photosynthetic state determine spatiotemporal variability of seasonal total gross primary productivity of vegetation. Recent warming induced impacts accelerate shifts on growing season and physiological status over Northern vegetated land. Thus, understanding and quantifying these changes are very important. Here, we first investigate how vegetation growing season and maximum photosynthesis state are evolved and how such components contribute on inter-annual variation of seasonal total gross primary productivity. Furthermore, seasonally different response of northern vegetation to changing temperature and water availability is also investigated. We utilized both long-term remotely sensed data to extract larger scale growing season metrics (growing season start, end and duration) and productivity (i.e., growing season summed vegetation index, GSSVI) for answering these questions. We find that regionally diverged growing season shift and maximum photosynthetic state contribute differently characterized productivity inter-annual variability and trend. Also seasonally different response of vegetation gives different view of spatially varying interaction between vegetation and climate. These results highlight spatially and temporally varying vegetation dynamics and are reflective of biome-specific responses of northern vegetation to changing climate.

Climate↗