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At least 811 records · Page 45
All sourcesNASA NTRSDOE OSTI
NASA NTRS2023

Structural Chokepoints Determine the Resilience of Agri-Food Supply Chains in the United States

The agricultural and food systems of the United States are critical for ensuring the stability of both domestic and global food systems. Thus, it is essential to understand the structural resilience of the country’s agri-food supply chains to a suite of threats. Here we employ complex network statistics to identify the spatially resolved structural chokepoints in the agri-food supply chains of the United States. We identify seven chokepoints at county scale: Riverside CA, San Bernardino CA, Los Angeles CA, Shelby TN, Maricopa AZ, San Diego CA and Cook IL; as well as seven chokepoints at freight analysis framework scale: Los Angeles–Long Beach CA, Chicago– Naperville IL, New York–New Jersey NJ, New York–New Jersey NY, Remainder of Texas, Remainder of Pennsylvania, and San Jose–San Francisco–Oakland CA. These structural chokepoints are generally consistent through time (2007, 2012, 2017), particularly for processed food commodities. This study improves our understanding of agri-food supply-chain security and may aid policies aimed at enhancing its resilience.

Agriculture↗
DOE OSTI2012

Neutrons measure phase behavior in pores at Angstrom size

Researchers have measured the phase behavior of green house gases in pores at the Angstrom-level, using small angle neutron scattering (SANS) at the Oak Ridge National Laboratory's High Flux Isotope Reactor. Yuri Melnichenko, an instrument scientist on the General Purpose Small Angle Neutron Scattering (GP SANS) Diffractometer at ORNL's High Flux Isotope Reactor, his postdoctoral associate Lilin He and collaborators Nidia Gallego and Cristian Contescu from the Material Sciences Division (ORNL) were engaged in the work. They were studying nanoporous carbons to assess their attractiveness as storage media for hydrogen, with a view to potential use for on-board hydrogen storage for transportation applications. Nanoporous carbons can also serve as electrode material for supercapacitors and batteries. The researchers successfully determined that the most efficiently condensing pore size in a carbon nanoporous material for hydrogen storage is less than one nanometer. In a paper recently published by the Journal of the American Chemical Society, the collaborators used small angle neutron scattering to study how hydrogen condenses in small pores at ambient temperature. They discovered that the surface-molecule interactions create internal pressures in pores that may exceed the external gas pressure by a factor of up to 50. 'This is an exciting result,' Melnichenko said, 'as you achieve extreme densification in pores 'for free', i.e. without spending any energy. These results can be used to guide the development of new carbon adsorbents tailored to maximize hydrogen storage capacities.' Another important factor that defines the adsorption capacity of sub-nanometer pores is their shape. In order to get accurate structural information and maximize sorption capacity, it is important that pores are small and of approximately uniform size. In collaboration with Drexel University's Yury Gogotsi who supplied the samples, Melnichenko and his collaborators used the GP SANS instrument to study how the size and shape of pores in sub-nanometer porous carbons varies, depending on the manufacturing conditions. While small angle X-ray scattering (SAXS) can do the job too, Melnichenko says, the SANS method broke new ground in analyzing the shape and behavior of pores at subnanometer size, when subjected to varying synthesis temperature. 'We found that these very small pores are in fact spherical, and that when we change the synthesis conditions, they become elongated, even 'slit-like', and all of this on a subnanometer scale,' Melnichenko said.

08 HYDROGEN↗
DOE OSTI2016

BENCHMARK ACCELERATED AGING OF HARVESTED HYPALON/EPR AND CSPE/XLPE POWER AND I&C CABLE IN NUCLEAR POWER PLANTS

As part of the Light Water Reactor and Sustainability (LWRS) program in the U.S. Department of Energy (DOE) Office of Nuclear Energy, material aging and degradation research is currently geared to support the long-term operation of existing nuclear power plants (NPPs) as they move beyond their initial 40 year licenses. The goal of this research is to provide information so that NPPs can develop aging management programs (AMPs) to address replacement and monitoring needs as they look to operate for 20 years, and in some cases 40 years, beyond their initial operating lifetimes. For cable insulation and jacket materials that support instrument, control, and safety systems, accelerated aging data are needed to determine priorities in cable aging management programs. Before accelerated thermal and radiation aging of harvested, representative cable insulation and jacket materials, the benchmark performance of a new test capability at Oak Ridge National Laboratory (ORNL) was evaluated for temperatures between 70 and 135 C, dose rates between 100 and 500 Gy/h, and accumulated doses up to 20 kGy, Samples that were characterized and are representative of current materials in use were harvested from the Callaway NPP near Fulton, Missouri, and the San Onofre NPP north of San Diego, California. From the Callaway NPP, a multiconductor control rod cable manufactured by Boston Insulated Wire (BIW), with a Hypalon/ chorolosulfonated polyethylene (CSPE) jacket and ethylene-propylene rubber (EPR) insulation, was harvested from the auxiliary space during a planned outage in 2013. This cable was placed into service when the plant was started in 1984. From the San Onofre NPP, a Rockbestos Firewall III (FRIII) cable with a Hypalon/ CSPE jacket with cross-linked polyethylene (XLPE) insulation was harvested from an on-site, climate-controlled storage area. This conductor, which was never placed into service, was procured around 2007 in anticipation of future operation that did not occur. Benchmark aging for both jacket and insulation material was carried out in air at a temperature of 125 C or in uniform 140 Gy/h gamma irradiation over a period of 60 days. Their mechanical properties over the course of their exposures were compared with reference data from comparable cable jacket/insulation compositions and aging conditions. For both accelerated thermal and radiation aging, it was observed that the mechanical properties for the Callaway BIW control rod cable were consistent with those previously measured. However, for the San Onofre Rockbestos FRIII, there was an observable functional difference for accelerated thermal aging at 125 C. Details on possible sources for this difference and plans for resolving each source are given in this paper.

Duckworth, Robert C↗
DOE OSTI2019

Stability of a Uranium-Contaminated Plume in a Karst Aquifer in Northwest New Mexico - 19574

The US Department of Energy (DOE) Office of Legacy Management monitors groundwater contamination resulting from ore processing operations conducted between 1953 and 1982 at a former uranium mill site on property that is now part of the Bluewater, New Mexico, Disposal Site. The site is located in the Grants Mineral Belt in northwest New Mexico at the base of the Zuni Mountains. It is estimated that prior to mill tailings encapsulation in 1995, approximately 21.6 billion liters (5.7 billion gallons) of tailings fluid seeped from the main tailings impoundment into the underlying aquifers. Today, there is remnant groundwater contamination from the mill in two aquifers: the San Andres-Glorieta (SAG) bedrock aquifer and the Ancestral Rio San Jose alluvial aquifer. Groundwater contamination in the SAG aquifer is of particular concern, as the aquifer is the primary water source in the region for municipal, agricultural, and industrial use. Municipal wells for the region are located as close as 6.4 kilometers (4 miles) southeast of the Main Tailings Disposal Cell. The SAG uranium plume extends east for more than 4.8 kilometers (3 miles) beyond the site's eastern boundary, along the direction of groundwater flow. The plume intersects the Homestake Mining Company site, another former uranium mill site undergoing active remediation. The SAG aquifer contains two formations, the Lower Permian San Andres Limestone and the Glorieta Sandstone. Most of the groundwater moving through the SAG aquifer is in ancient karst features consisting of open conduits where the limestone has dissolved, resulting in flow through solution cavities, solution channels, cavernous zones, and enlarged fracture openings. These features make the San Andres Limestone extremely permeable and capable of rapidly transporting groundwater. In addition, the karst characteristics make the aquifer difficult to evaluate and the uranium plume difficult to characterize. Numerous faults in the area, including two directly under the Main Tailings Disposal Cell, further complicate characterization by impacting groundwater flow both onsite and in the vicinity. A DOE study of the SAG aquifer uranium plume at the Bluewater site, conducted in 2014, indicated that the plume had moved offsite soon after the start of milling and effectively stabilized in its present location prior to the 1980's. An updated study completed in 2018 confirmed previous information regarding general plume stability and provided insight regarding potential localized trends east and south of the Main Tailings Disposal Cell. One such trend may be decreasing uranium concentrations east of the Main Tailings Disposal Cell, potentially attributable to regional groundwater elevation decreases. Both the 2013 and 2017 plumes suggest that the dissolved uranium contamination is derived from the leaching of a secondary mineralized zone derived from seeped tailings fluids in the geologic formations and fault zones below the Main Tailings Disposal Cell at the Bluewater site. In developing the 2013 and 2017 SAG aquifer uranium plume depictions, DOE had to address challenges associated with data quality and comparability. Analytical results used to determine the plume extent were compiled from multiple sources, including the state of New Mexico, Homestake Mining Company, and DOE. Each source used different well identifiers and coordinate systems, and well information between sources was inconsistent. Also, the integrity of some wells was questionable and necessitated review; casing integrity was found to be compromised in multiple wells. Given these uncertainties, some of these wells were excluded from the characterization. Another source of uncertainty is the paucity of wells, particularly monitoring wells, near or immediately downgradient of the leading edge of the plume. Therefore, the exact extent of the SAG aquifer uranium plume is not known at this time. There is, however, no evidence or indication of milling-related contamination in municipal wells. Furthermore, due to plume stability and direction of groundwater flow, the municipal wells are not expected to be impacted. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2023

Skyrmion‐Excited Spin‐Wave Fractal Networks

Magnetic skyrmions exhibit unique, technologically relevant pseudo-particle behaviors which arise from their topological protection, including well-defined, 3D dynamic modes that occur at microwave frequencies. During dynamic excitation, spin waves are ejected into the interstitial regions between skyrmions, creating the magnetic equivalent of a turbulent sea. However, since the spin waves in these systems have a well-defined length scale, and the skyrmions are on an ordered lattice, ordered structures from spin-wave interference can precipitate from the chaos. This work uses small-angle neutron scattering (SANS) to capture the dynamics in hybrid skyrmions and investigate the spin-wave structure. Performing simultaneous ferromagnetic resonance and SANS, the diffraction pattern shows a large increase in low-angle scattering intensity, which is present only in the resonance condition. This scattering pattern is best fit using a mass fractal model, which suggests the spin waves form a long-range fractal network. The fractal structure is constructed of fundamental units with a size that encodes the spin-wave emissions and are constrained by the skyrmion lattice. Furthermore, these results offer critical insights into the nanoscale dynamics of skyrmions, identify a new dynamic spin-wave fractal structure, and demonstrate SANS as a unique tool to probe high-speed dynamics.

36 MATERIALS SCIENCE↗
DOE OSTI2017

New Polarized Small Angle Neutron Scattering capability at the High Flux Isotope Reactor

Polarized Small Angle Neutron Scattering (SANS) is a powerful tool for studying mesoscale magnetic structures. In a recent development at the Oak Ridge National Laboratory (ORNL), a polarized neutron beam has been implemented for the CG-2 (GP-SANS) beamline at the High Flux Isotope Reactor (HFIR). Three major components were developed to provide the polarized neutron capability: a transmission supermirror neutron polarizer, an adiabatic fast passage neutron spin flipper and a polarized 3 He filter as neutron spin analyzer. Finally, the new polarized beam SANS capability was used to investigate several magnetic samples having mesoscale structures.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2013

A portable hydro-thermo-mechanical loading cell for in-situ small angle neutron scattering studies of proton exchange membranes

A portable hydro-thermo-mechanical loading cell has been designed to enable in-situ small angle neutron scattering (SANS) studies of proton exchange membranes (PEM) under immersed tensile loadings at different temperatures. The cell consists of three main parts as follows: a letter-paper-size motor-driven mechanical load frame, a SANS friendly reservoir that provides stable immersed and thermal sample conditions, and a data acquisition & control system. The ex-situ tensile tests of Nafion 212 membranes demonstrated a satisfactory thermo-mechanical testing performance of the cell for either dry or immersed conditions at elevated temperatures. The in-situ SANS tensile measurements on the Nafion 212 membranes immersed in D2O at 70oC proved the feasibility and capability of the cell for small angle scattering study on deformation behaviors of PEM and other polymer materials under hydro-thermo-mechanical loading.

hydro-thermo-mechanical.↗
DOE OSTI2021

Time-of-flight modulated intensity small-angle neutron scattering measurement of the self-diffusion constant of water

We report that the modulated intensity by zero effort small-angle neutron scattering (MI-SANS) technique is used to measure scattering with a high energy resolution on samples normally ill-suited for neutron resonance spin echo. The self-diffusion constant of water is measured over a q–t range of 0.01–0.2 Å -1 and 70–500 ps. In addition to demonstrating the methodology of using time-of-flight MI-SANS instruments to observe diffusion in liquids, the results support previous measurements on water performed with different methods. This polarized neutron technique simultaneously measures the intermediate scattering function for a wide range of time and length scales. Two radio frequency flippers were used in a spin-echo setup with a 100 kHz frequency difference in order to create a high-resolution time measurement. The results are compared with self-diffusion measurements made by other techniques and the general applicability of MI-SANS at a pulsed source is assessed.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2024

A science-driven approach to optimize the design for a biological small-angle neutron scattering instrument

Biological small-angle neutron scattering (SANS) instruments facilitate critical analysis of the structure and dynamics of complex biological systems. However, with the growth of experimental demands and the advances in optical systems design, a new neutron optical concept is necessary to overcome the limitations of current instruments. This work presents an approach to include experimental objectives ( i.e. the science to be supported by a specific neutron scattering instrument) in the optimization of the neutron optical concept. The approach for a proposed SANS instrument at the Second Target Station of the Spallation Neutron Source at Oak Ridge National Laboratory, USA, is presented here. Further, the instrument is simulated with the McStas software package. The optimization process is driven by an evolutionary algorithm using McStas output data, which are processed to calculate an objective function designed to quantify the expected performance of the simulated neutron optical configuration for the intended purpose. Each McStas simulation covers the complete instrument, from source to detector, including realistic sample scattering functions. This approach effectively navigates a high-dimensional parameter space that is otherwise intractable; it allows the design of next-generation SANS instruments to address specific scientific cases and has the potential to increase instrument performance compared with traditional design approaches.

47 OTHER INSTRUMENTATION↗
DOE OSTI2022

The importance of phonon spectrum measurements for accurate prediction of thermal scattering laws [Slides]

Although approximate phonon spectra (e.g. Debye model) are sufficient to reproduce integral quantities (e.g. total INS cross section) in reasonable agreement with the measured data, more accurate phonon spectra are needed to achieve agreement with the differential data. SANS component is essential for reproducing the total scattering cross section; this is the case for nanodiamonds and other materials where SANS components are present (such as porous materials like nuclear graphite). A format proposal for SANS data in ENDF or GNDS is needed. Therefore, differential (e.g. phonon spectrum) and integral (e.g. total cross section) measurements are necessary in the process of creating an ENDF evaluation. We have successfully developed a method by which ENDF evaluations can be directly compared to the relevant experimentally measured quantities. INS experiments at SNS, ILL, ISIS, and, in the future, ESS, as well as total cross section measurements at the VESUVIO instrument at ISIS, are accessible through user programs.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
NASA NTRS2016

Retrieval of Daily Maps of PM2.5 Aerosol in the Problematic California Valleys: Bright, Speckled Reflectances, Thin AOT, but High Pollution

The San Joaquin Valley suffers from severe episodes of respirable aerosol (PM2.5) in wintertime.We provide maps of aerosol episodes using daily snapshots of PM2.5 and its changing features despite numerous difficulties inherent to sampling the region. Linear relationships relating aerosol optical thickness (AOT) to PM2.5 give an explained variance of approximately 3.The GEO-CAPE mission has as a goal the provision of relevant measures of respirable aerosol to the community,but has not formulated a science goal beyond the limited goal of retrieval of AOT, bringing the usefulness of GEO-CAPE into doubt.Our special focus was on the DISCOVER-AQ period, Jan-Feb 2013, which had many supporting measurements.Both high pollution and retrieval difficulties tend to occur in many Mediterranean agricultural regions like the San Joauin. One difficulty is the relatively bright surfaces with considerable exposed soil. NASAs MAIAC and MODIS Deep Blue retrieval techniques are shown to have considerable skill even at low aerosol optical thickness (AOT) values, as evaluated by concurrent AERONET sunphotometer measurements.More significantly, these AOT values can correspond to high daytime PM2.5 since aerosol mixed layer depth is thin and variable, 200m 600 m. The thin layers derive from typical subsidence of dry air between more stormy periods. This situation provides an advantage: water vapor column is also almost completely limited to a similar mixed layer depth, and can thus serve as a measure of aerosol dilution.Using the MAIAC Water Vapor Column:In order to make the maps below, we used the MAIAC data but subtracted partial water-vapor columns estimated from MERRA Reanalysis Data availabe from the GSFC GMAO using kriging. We did not use the mixed-layer estimates from MERRA, since such analyses were found problematic during our forecasting exercises for DISCOVER-AQ. Observations from the aircraft soundings suggested that this overlying moisture was mostly due to larger scale flows, not ML venting.However, the specific humidity at the surface and a nearly well-mixed ML was analyzed by kriging from the surface network (MesoWest, University of Utah). These were thought to be truer, uninfluenced by physical process modeling that combines with data observations. (TBD: How different are they?) Procedure: Subtract overlying partial water columns from MAIAC column water and divide this by a surface value of water vapor. (MAIAC column is expressed in cm of water, i.e., water vapor at surface conditions.This method appears to bring out useful details in the distribution of submicron particles in the very problematic Wintertime San Joaquin Valley, and allow analysis of pollution episodes throughout the valley, rather than long-term averages.

san joaquin valley↗
DOE OSTI2018

Sea Level Rise Impacts on Wastewater Treatment Systems Along the U.S. Coasts

As sea levels rise, coastal communities will experience more frequent and persistent nuisance flooding, and some low-lying areas may be permanently inundated. Critical components of lifeline infrastructure networks in these areas are also at risk of flooding, which could cause significant service disruptions that extend beyond the flooded zone. Thus, identifying critical infrastructure components that are exposed to sea level rise is an important first step in developing targeted investment in protective actions and enhancing the overall resilience of coastal communities. Wastewater treatment plants are typically located at low elevations near the coastline to minimize the cost of collecting consumed water and discharging treated effluent, which makes them particularly susceptible to coastal flooding. For this analysis, we used geographic information systems to assess the exposure of wastewater infrastructure to various sea level rise projections at the national level. We then estimated the number of people who would lose wastewater services, which could be more than five times as high as previous predictions of the number of people at risk of direct flooding due to sea level rise. We also performed a regional comparison of wastewater exposure to marine and groundwater flooding in the San Francisco Bay Area. Overall, this analysis highlights the widespread exposure of wastewater infrastructure in the United States and demonstrates that local disruptions to infrastructure networks may have far-ranging impacts on areas that do not experience direct flooding.Plain Language Summary Wastewater treatment plants are susceptible to flooding resulting from sea level rise. Previous estimates of wastewater exposure have only considered the impacts of marine flooding at the local or regional scale. In this analysis, we quantify the exposure to marine flooding across the coastal United States and then consider the relative impacts of marine and groundwater flooding at the regional scale in the San Francisco Bay Area. We also estimate the number of people who may lose access to wastewater services if no actions are taken to prevent flooding at wastewater treatment plants. We find that the number of people impacted by sea level rise due to loss of wastewater services could be five times as high as previous predictions of the number of people who experience direct flooding of their homes or property. We also find that groundwater flooding poses a significant threat to wastewater plants in the San Francisco Bay region.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Probing the interface structure of block copolymer compatibilizers in semicrystalline polymer blends

Abstract The use of block copolymers to compatibilize immiscible plastics is an important strategy for upcycling municipal plastic wastes. Multiblock copolymers (MBCPs) have been proven to be more effective compatibilizers than di‐ and tri‐block copolymers. Herein, we probe the interface structure of an effective multiblock copolymer compatibilizer and compare that with an ineffective triblock copolymer (TBCP). The interface activity of the compatibilizers is understood through a combination of small‐angle neutron and x‐ray scatterings (SANS and SAXS), by using deuterated homopolymer matrix and protonated compatibilizers. SANS analysis suggests that the MBCP forms a thicker interface layer (7–9 nm) than the TBCP (0–4 nm). In addition, SANS data seems to point to a stronger tendency for the MBCP to locate at the interface. Both factors contribute to its effectiveness at compatibilizing immiscible homopolymers.

36 MATERIALS SCIENCE↗
DOE OSTI2022

Determination of the Structures of Lignin Subunits and Nanoparticles in Solution by Small‐Angle Neutron Scattering: Towards Improving Lignin Valorization

Abstract Lignin nanoparticles (LNPs) are usually produced from lignin solution through supersaturation. The structure of the lignin in solution is still poorly understood due to structural variability of isolated lignins. Here, lignins were extracted from different plants to establish a general pattern of their structure in several lignin solvents. Lignin molecules (lignin subunits) and larger aggregates were observed in dimethyl sulfoxide (DMSO), ethylene glycol (EG) and 0.1 N NaOD solutions by small‐angle neutron scattering (SANS). It was proposed that the aggregates were composed of lignin subunits with a higher molecular weight and a higher ratio of the aliphatic to phenolic hydroxyl groups. The size, shape, and compactness are important factors that affect the uses of the LNPs, which were obtained from the SANS data for the first time. A discrepancy in the radius between SANS and DLS was discovered, pointing to a large hydration shell around the LNPs in aqueous solutions. The cytotoxicity of the corncob lignin, kraft lignin, and their LNPs were measured and compared.

Zhang, Xin↗
DOE OSTI2021

Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor‐III System‐Based Extraction

Abstract The membrane proteins (MP) α‐synuclein (ASYN) and bacteriorhodopsin (BR) were readily incorporated into bicontinuous microemulsions (BμEs) formed by two microemulsion systems: water/heptane/Aerosol‐OT (AOT)/CK‐2,13 and water/dodecane/sodium dodecyl sulfate (SDS)/1‐pentanol. (CK‐2,13 is an alkyl ethoxylate possessing two alkyl tail groups of carbon chain length 2 and 13 and an average degree of ethoxylation of 5.6.) MP were encapsulated in BμEs through preparation of Winsor‐III systems at optimal salinity, with the anionic surfactants AOT and SDS providing the driving force for extraction. Dissolution of ASYN in BμEs greatly increased the former's α‐helicity, similar to ASYN's behavior in the presence of biomembranes, while BμE‐ and vesicle‐encapsulated BR possessed similar secondary structure. Small‐angle neutron scattering (SANS) results clearly demonstrated the direct interaction of MP with the surfactants, resulting in a decrease of surface area per volume for surfactant monolayers due to decreased surfactant efficiency. The SANS signal for ASYN was isolated through the use of neutron contrast matching for the surfactants through partial deuteration of water and oil, one of the first reports of contrast matching for BμEs in the literature. The SANS results of the contrast‐matched sample reflected similar aggregation for ASYN in BμEs as was reported previously for vesicles and SDS solution. This study demonstrates the potential use of BμEs as MP host systems for conducting biochemical reactions such as the conversion of sunlight into adenosine triphosphate by BR and studying the fundamental behavior of MP, such as the role of ASYN dysfunction in Parkinson's disease, as well as for isolation and purification of MP via Winsor‐III‐based extraction.

Hayes, Douglas G.↗
DOE OSTI2020

Cell fusing agent virus (Flavivirus) infection in Aedes aegypti in Texas: seasonality, comparison by trap type, and individual viral loads

Abstract South Texas has experienced local transmission of Zika virus and of other mosquito-borne viruses such as chikungunya virus and dengue virus in the last decades. Using a mosquito surveillance program in the Lower Rio Grande Valley (LRGV) and San Antonio, TX, from 2016 to 2018, we detected the presence of an insect-specific virus, cell fusing agent virus (CFAV), in the Aedes aegypti mosquito population. We tested 6,326 females and 1,249 males from the LRGV and 659 females from San Antonio for CFAV by RT-PCR using specific primers. Infection rates varied from 0 to 261 per 1,000 mosquitoes in the LRGV and 115 to 208 per 1,000 in San Antonio depending on the month of collection. Infection rates per 1,000 individuals appeared higher in females collected from BG Sentinel 2 traps compared to Autocidal Gravid Ovitraps, but the ratio of the percentage of infected pools did not differ by trap type. The natural viral load in individual males ranged from 1.25 x 10 2 to 5.50 x 10 6 RNA copies and in unfed females from 5.42 x 10 3 to 8.70 x 10 6 RNA copies. Gravid females were found to harbor fewer viral particles than males and unfed females.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Neutron scattering in photosynthesis research: recent advances and perspectives for testing crop plants

The photosynthetic performance of crop plants under a variety of environmental factors and stress conditions, at the fundamental level, depends largely on the organization and structural flexibility of thylakoid membranes. These highly organized membranes accommodate virtually all protein complexes and additional compounds carrying out the light reactions of photosynthesis. Most regulatory mechanisms fine-tuning the photosynthetic functions affect the organization of thylakoid membranes at different levels of the structural complexity. In order to monitor these reorganizations, non-invasive techniques are of special value. On the mesoscopic scale, small-angle neutron scattering (SANS) has been shown to deliver statistically and spatially averaged information on the periodic organization of the thylakoid membranes in vivo and/or, in isolated thylakoids, under physiologically relevant conditions, without fixation or staining. More importantly, SANS investigations have revealed rapid reversible reorganizations on the timescale of several seconds and minutes. In this paper, we give a short introduction into the basics of SANS technique, advantages and limitations, and briefly overview recent advances and potential applications of this technique in the physiology and biotechnology of crop plants. We also discuss future perspectives of neutron crystallography and different neutron scattering techniques, which are anticipated to become more accessible and of more use in photosynthesis research at new facilities with higher fluxes and innovative instrumentation.

59 BASIC BIOLOGICAL SCIENCES↗