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At least 811 records · Page 45

6 th International Conference on Plant Synthetic Biology, Bioengineering and Biotechnology (Workshop Report)

The objective of the project is to hold a Conference to bring together scientists and engineers from universities, industry and government working in all aspects of plant synthetic biology, plant bioengineering and plant biotechnology. To meet this objective, AIChE will host the 6th International Conference on Plant Synthetic Biology, Bioengineering and Biotechnology on December 9-11, 2022 in Fort Lauderdale, Florida. The conference will feature five technical sessions: (1) Genome and Epigenome Editing, (2) Specialized Metabolism, (3) Engineering Plant Resilience, (4) Engineering more efficient plants, and (5) New tools and Technologies. The conference will allow leading scientists and engineers from universities, industry and government to discuss new tools and technologies being developed as well as their applications, and gaps in the field. The conference will allow students to learn about these new technologies, engage in a discussion with professionals in their field of interest, and showcase their work. The scope, theme, and aims of the Conference on Plant Synthetic Biology, Bioengineering, and Biotechnology fall squarely within the mission of DOE which includes securing US leadership in energy technology and to maintaining a vibrant US effort in science and engineering as a cornerstone for our economic prosperity.

59 BASIC BIOLOGICAL SCIENCES↗

An open-access simulated earthquake ground-motion database for an M7 Hayward Fault earthquake in the San Francisco Bay Region

Comprehensive understanding of earthquake ground motions, particularly in the near-fault region of large-magnitude events, is limited by gaps in strong-motion data. This challenge is prominent in areas with high seismic hazard but infrequent large earthquakes where data is sparse and difficult to interpret. These data limitations lead to uncertainties in the development of site-specific ground motions, which are crucial for engineering risk assessments. To address these challenges, physics-based regional-scale ground-motion simulations have been developed. With the emergence of exaflop-scale computing ecosystems, it is now possible to simulate regional earthquake processes at unprecedented fidelity and generate the large number of fault rupture realizations necessary to characterize both intra- and inter-event ground-motion variability. This article introduces a new database of simulated earthquake ground motions, created for applications in earthquake engineering, earthquake planning, and emergency response. The inaugural version of the database features simulated ground motions for a magnitude 7 Hayward Fault earthquake in the San Francisco Bay Region (SFBR), using the EarthQuake SIMulation (EQSIM) simulation framework and the Graves–Pitarka kinematic rupture model. The aim is to provide high-fidelity, spatially dense, three-component motions generated on the Department of Energy’s (DOE) newest generation of graphics processing unit (GPU)-accelerated supercomputers. These motions are being made openly available to the engineering, scientific, and disaster planning communities. In addition, this work develops protocols for the efficient dissemination of these large data sets and emphasizes community engagement to build confidence in their application. This article discusses the methodology behind the data, underlying software verification and validation, scalable data management, and a user interface for data access. The goal is to facilitate widespread use and elicit expert feedback to maximize the utility and exploitation of simulated motions. While the initial focus is on the San Francisco Region, simulations for additional regions will be added as the DOE program progresses.

Simulated ground-motion database↗

ClusterCAD 2.0: an updated computational platform for chimeric type I polyketide synthase and nonribosomal peptide synthetase design

Abstract Megasynthase enzymes such as type I modular polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) play a central role in microbial chemical warfare because they can evolve rapidly by shuffling parts (catalytic domains) to produce novel chemicals. If we can understand the design rules to reshuffle these parts, PKSs and NRPSs will provide a systematic and modular way to synthesize millions of molecules including pharmaceuticals, biomaterials, and biofuels. However, PKS and NRPS engineering remains difficult due to a limited understanding of the determinants of PKS and NRPS fold and function. We developed ClusterCAD to streamline and simplify the process of designing and testing engineered PKS variants. Here, we present the highly improved ClusterCAD 2.0 release, available at https://clustercad.jbei.org. ClusterCAD 2.0 boasts support for PKS-NRPS hybrid and NRPS clusters in addition to PKS clusters; a vastly enlarged database of curated PKS, PKS-NRPS hybrid, and NRPS clusters; a diverse set of chemical ‘starters’ and loading modules; the new Domain Architecture Cluster Search Tool; and an offline Jupyter Notebook workspace, among other improvements. Together these features massively expand the chemical space that can be accessed by enzymes engineered with ClusterCAD.

59 BASIC BIOLOGICAL SCIENCES↗

Space Shuttle Main Engine: Advanced Health Monitoring System

The main gola of the Space Shuttle Main Engine (SSME) Advanced Health Management system is to improve flight safety. To this end the new SSME has robust new components to improve the operating margen and operability. The features of the current SSME health monitoring system, include automated checkouts, closed loop redundant control system, catastropic failure mitigation, fail operational/ fail-safe algorithms, and post flight data and inspection trend analysis. The features of the advanced health monitoring system include: a real time vibration monitor system, a linear engine model, and an optical plume anomaly detection system. Since vibration is a fundamental measure of SSME turbopump health, it stands to reason that monitoring the vibration, will give some idea of the health of the turbopumps. However, how is it possible to avoid shutdown, when it is not necessary. A sensor algorithm has been developed which has been exposed to over 400 test cases in order to evaluate the logic. The optical plume anomaly detection (OPAD) has been developed to be a sensitive monitor of engine wear, erosion, and breakage.

Singer, Chirs↗

Providing Long-term Solar and Meteorological Resource Information from NASA Research through the GIS-Enabled POWER Web Services Portal

Solar and other renewable energy systems are optimized using geophysical parameters describing information about the solar resource and meteorological environments and how those environments may be changing in time. The Prediction of Worldwide Renewable Energy Resource (POWER) team at the NASA LaRC Research Center deployed its first version of the revitalized POWER geophysical parameter website that employs Esri Geographic Information System (GIS) tools. This web application provides access to both time series and climatological data sets spanning from a few days behind real-time back to the early 1980’s with a spatial resolution of 0.5 x 0.5 degree, thus extending over 30 years. The GIS tools enable, generate and store climatological averages using spatial queries and calculations in a spatial database resulting in greater accessibility to government agencies, industry and individuals. There is an API that provides data through a URL coding and can also invoked inside user software packages. Output data formats now include ASCII, CSV, geoTiff, JSON and .netCDF. Since May 2018, over 10.4 TB of data have been delivered to fulfill over 110 million data requests from 240,000 unique user IPs (https://power.larc.nasa.gov). This presentation provides an overview of this project and the current version of the new POWER web capabilities through to the end usage. Surface solar parameters are adapted from both the NASA/GEWEX (Global and Energy Water Cycle Exchange) SRB (Surface Radiation Budget) project and the CERES (Clouds and Earth’s Radiant Energy System) data products. The meteorological parameters are adapted from NASA’s MERRA-2 (Modern Era Retrospective-analysis for Research and Applications). We review uncertainty of various basic parameters using surface measurements. After the introduction, we specifically discuss various clusters of parameters for equator pointing tilted surfaces that provide for the estimation of optimal tilt angle by month and year. The discussion will emphasize a few examples of how solar and building engineers are using the data products. We will then preview the capabilities of Version 2 featuring examples of hourly solar and meteorological data products, customized user reports and expanded web mapping services from CERES data products. The web services provide a unique, expandable resource for renewable energy systems engineers to design, evaluate and optimize to environments worldwide.

Solar Resource↗

Conventional engine technology. Volume 2: Status of diesel engine technology

The engines of diesel cars marketed in the United States were examined. Prominent design features, performance characteristics, fuel economy and emissions data were compared. Specific problems, in particular those of NO and smoke emissions, the effects of increasing dieselization on diesel fuel price and availability, current R&D work and advanced diesel concepts are discussed. Diesel cars currently have a fuel economy advantage over gasoline engine powered cars. Diesel drawbacks (noise and odor) were reduced to a less objectionable level. An equivalent gasoline engine driveability was obtained with turbocharging. Diesel manufacturers see a growth in the diesel market for the next ten years. Uncertainties regarding future emission regulation may inhibit future diesel production investments. With spark ignition engine technology advancing in the direction of high compression ratios, the fuel economy advantages of the diesel car is expected to diminish. To return its fuel economy lead, the diesel's potential for future improvement must be used.

Schneider, H. W.↗

Development of a Fabry-Perot interferometer for rocket engine plume monitoring

The development of a lightweight, compact, high-resolution Fabry-Perot interferometer (FPI) based spectrometer capable of detecting the spectral signatures of eroding engine components during test and/or flight operations is discussed. FPI based spectrometers will be designed to be smaller and lighter than grating or prism type devices and to provide greater wavelength resolving capability. The FPI system seeks to combine the features of high line resolution, active background discrimination, smart digital signal processing, and rocket engine flight capability. The design, fabrication, and test of a breadboard FPI spectrometer have been completed and the breadboard instrument has clearly demonstrated the viability of the approach. The breadboard instrument design and test results are also presented.

Bickford, R. L.↗

Macroscopic study of time unsteady noise of an aircraft engine during static tests

Static tests of aircraft engines can exhibit greater than 10 db random unsteadiness of tone noise levels because flow disturbances that prevail near test site facilities are ingested. Presumably such changes are related to installation and test site features. Some properties of unsteady noise observed during tests of a Lycoming YF-102 turbofan engine are presented. Time and spatial variations in tone noise obtained from closely spaced far field and inlet duct microphones are displayed. Long to extremely short intermittent tone bursts are observed. Unsteadiness of the tone, its harmonics, and the broadband noise show little similarity. In the far field, identity of tone bursts is retained over a directivity angle of less than 10 deg. In the inlet duct, tone bursts appear to propagate axially but exhibit little circumferential similarity. They show only slight relationship to tone bursts observed in the far field. The results imply an intermittent generation of random mixtures of propagating duct modes.

Clark, B. J.↗

Printability and Overall Cooling Performance of Additively Manufactured Holes With Inlet and Exit Rounding

Abstract To improve cooling effectiveness of gas turbine hardware, various film cooling hole shapes have previously been researched. Unique design modifications have recently been made possible through the design freedom allotted by additive manufacturing (AM). As one example, creating a rounded inlet for a film-cooling hole can mitigate separation at the inlet. This study explores various geometric features by exploiting the uses of additive manufacturing for shaped film cooling holes at engine scale. Both printability and cooling performance were evaluated. Resulting from this study, additively manufactured holes with hole inlet and exit rounding were printed with some variations from the design intent (DI). The largest deviations from the design intent occurred from dross roughness features located on the leeward side of the hole inlet. The measured overall effectiveness indicated that an as-built inlet fillet decreased in-hole convection as well as decreased jet mixing compared to the as-built sharp inlet. Including an exit fillet, which prevented an overbuilt diffuser exit, was also found to decrease jet mixing. A particular insight gained from this study is the importance of the convective cooling within the hole to the overall cooling performance. In-hole roughness, which is a result of additive manufacturing, increased convective cooling within the holes but also increased jet mixing as the coolant exited the hole. The increased jet mixing caused low overall effectiveness downstream of injection.

Engineering↗

Crashworthy airframe design concepts: Fabrication and testing

Crashworthy floor concepts applicable to general aviation aircraft metal airframe structures were investigated. Initially several energy absorbing lower fuselage structure concepts were evaluated. Full scale floor sections representative of a twin engine, general aviation airplane lower fuselage structure were designed and fabricated. The floors featured an upper high strength platform with an energy absorbing, crushable structure underneath. Eighteen floors were fabricated that incorporated five different crushable subfloor concepts. The floors were then evaluated through static and dynamic testing. Computer programs NASTRAN and KRASH were used for the static and dynamic analysis of the floor section designs. Two twin engine airplane fuselages were modified to incorporate the most promising crashworthy floor sections for test evaluation.

Cronkhite, J. D.↗

A Probabilistic System Analysis of Intelligent Propulsion System Technologies

NASA s Intelligent Propulsion System Technology (Propulsion 21) project focuses on developing adaptive technologies that will enable commercial gas turbine engines to produce fewer emissions and less noise while increasing reliability. It features adaptive technologies that have included active tip-clearance control for turbine and compressor, active combustion control, turbine aero-thermal and flow control, and enabling technologies such as sensors which are reliable at high operating temperatures and are minimally intrusive. A probabilistic system analysis is performed to evaluate the impact of these technologies on aircraft CO2 (directly proportional to fuel burn) and LTO (landing and takeoff) NO(x) reductions. A 300-passenger aircraft, with two 396-kN thrust (85,000-pound) engines is chosen for the study. The results show that NASA s Intelligent Propulsion System technologies have the potential to significantly reduce the CO2 and NO(x) emissions. The results are used to support informed decisionmaking on the development of the intelligent propulsion system technology portfolio for CO2 and NO(x) reductions.

Tong, Michael T.↗

The "Universal propeller": built by Paragon Engineers, Inc., Baltimore, Md

At the request of the N.A.C.A. the "Universal Propeller" was operated and explained by the inventor, Mr. Spencer Heath, for the purpose of demonstrating the following features of design: 1) Elimination of continuously running gears, collars or bearings in the pitch control mechanism; 2) The use of engine power in place of manual labor in changing blade angle; 3) The absence of any structural limitation to the range of blade angles available and the possibility of limiting the blade travel between any two predetermined extreme positions; 4) Continuous indication on the instrument board of the blade position; 5) Automatic throttling of the engine while the propeller is passing through the position of neutral pitch.

PROPELLERS, CONTROLLABLE - PITCH↗

Cylinder cooling in opposed-piston engines

A cylinder assembly with a cylinder liner and a sleeve is provided that includes features that reduce coolant flow stagnation. The sleeve encloses a center section of the cylinder liner to form cooling channels that removes excess heat from the combustion area of the cylinder. The cylinder liner includes features for cooling between bridges in the cylinder's exhaust port.

Sahasrabudhe, Abhishek↗

Development and Validation of Physics-Based Sub-Models of High-Pressure Supercritical Fuel Injection at Diesel Conditions Insert

The project sought to develop and validate accurate, physics-based, numerical submodels and implement them in computational fluid dynamics (CFD) software codes to enable accurate predictions of diesel sprays at pressures higher than the current systems. The combined experimental-computational effort has four main objectives: (1) acquire spatially and temporally resolved scalar measurements of fuel-oxidizer mixing in the near field of the fuel jet, (2) develop software modules to calculate thermo-physical properties of diesel surrogates and their mixtures with oxidizer of interest in real-gas operating regime, (3) integrate the real-fluid model into a commercial and an open-source CFD code to simulate fuel-air mixing in high-pressure diesel sprays, and (4) assess the robustness, accuracy, and uncertainty of the integrated CFD solvers in computing diesel sprays. Project accomplishments are consistent with the original goals of the project. Although we acquired spatially and temporally resolved scalar measurements, they were limited to refractive index difference and not to thermodynamic properties such as temperature and equivalence ratio. However, we successfully developed the theoretical background needed to acquire such measurements at diesel conditions and demonstrated the capability of the RSD for scalar measurements in a canonical turbulent jet flow replicating features of the diesel jet. Additional work will be needed to implement these procedures at high-pressure, high-temperature engine-like conditions of the CPFR. Mainly, we did not anticipate the need to find the liquid boundary and its statistical features or the state relationships between refractive index and thermodynamic properties, assuming the widely known Dale-Gladstone equation would suffice. Still, efforts devoted to these tasks were essential to fulfil the goals of the project and serves as the foundation on which many of the conclusions and key contributions of this project are based. Further assessment and application of the developed CFD codes is recommended to fully realize the benefits of this research.

33 ADVANCED PROPULSION SYSTEMS↗

Study of small turbofan engines applicable to single-engine light airplanes

The design, efficiency and cost factors are investigated for application of turbofan propulsion engines to single engine, general aviation light airplanes. A companion study of a hypothetical engine family of a thrust range suitable to such aircraft and having a high degree of commonality of design features and parts is presented. Future turbofan powered light airplanes can have a lower fuel consumption, lower weight, reduced airframe maintenance requirements and improved engine overhaul periods as compared to current piston engined powered airplanes. Achievement of compliance with noise and chemical emission regulations is expected without impairing performance, operating cost or safety.

Merrill, G. L.↗

Kokkos v.4.0

SAND2023-07883O Kokkos software implements C++ performance portability programming models, tools and math libraries, which enables science and engineering software developers to use single-source codes for a wide range of computer architectures. Kokkos also provides implementations of existing and proposed C++ standard features that support programming model and math libraries that are used for implementing performance-portable scientific and engineering applications. The Kokkos libraries provide algorithms, data structures, and tools to enable high-performance computing developers to write performance-portable code. Capabilities fall into three broad categories: Kokkos Core, Kokkos Kernels, and Kokkos Tools. Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc., for the U.S. Department of Energy’s National Nuclear Security Administration under contract DE-NA0003525.

SciDAC↗

Free-piston Stirling engine conceptual design and technologies for space power, phase 1

As part of the SP-100 program, a phase 1 effort to design a free-piston Stirling engine (FPSE) for a space dynamic power conversion system was completed. SP-100 is a combined DOD/DOE/NASA program to develop nuclear power for space. This work was completed in the initial phases of the SP-100 program prior to the power conversion concept selection for the Ground Engineering System (GES). Stirling engine technology development as a growth option for SP-100 is continuing after this phase 1 effort. Following a review of various engine concepts, a single-cylinder engine with a linear alternator was selected for the remainder of the study. The relationships of specific mass and efficiency versus temperature ratio were determined for a power output of 25 kWe. This parametric study was done for a temperature ratio range of 1.5 to 2.0 and for hot-end temperatures of 875 K and 1075 K. A conceptual design of a 1080 K FPSE with a linear alternator producing 25 kWe output was completed. This was a single-cylinder engine designed for a 62,000 hour life and a temperature ratio of 2.0. The heat transport systems were pumped liquid-metal loops on both the hot and cold ends. These specifications were selected to match the SP-100 power system designs that were being evaluated at that time. The hot end of the engine used both refractory and superalloy materials; the hot-end pressure vessel featured an insulated design that allowed use of the superalloy material. The design was supported by the hardware demonstration of two of the component concepts - the hydrodynamic gas bearing for the displacer and the dynamic balance system. The hydrodynamic gas bearing was demonstrated on a test rig. The dynamic balance system was tested on the 1 kW RE-1000 engine at NASA Lewis.

Penswick, L. Barry↗

Multiscale embedded printing of engineered human tissue and organ equivalents

Creating tissue and organ equivalents with intricate architectures and multiscale functional feature sizes is the first step toward the reconstruction of transplantable human tissues and organs. Existing embedded ink writing approaches are limited by achievable feature sizes ranging from hundreds of microns to tens of millimeters, which hinders their ability to accurately duplicate structures found in various human tissues and organs. In this study, a multiscale embedded printing (MSEP) strategy is developed, in which a stimuli-responsive yield-stress fluid is applied to facilitate the printing process. A dynamic layer height control method is developed to print the cornea with a smooth surface on the order of microns, which can effectively overcome the layered morphology in conventional extrusion-based three-dimensional bioprinting methods. Since the support bath is sensitive to temperature change, it can be easily removed after printing by tuning the ambient temperature, which facilitates the fabrication of human eyeballs with optic nerves and aortic heart valves with overhanging leaflets on the order of a few millimeters. The thermosensitivity of the support bath also enables the reconstruction of the full-scale human heart on the order of tens of centimeters by on-demand adding support bath materials during printing. Here, the proposed MSEP demonstrates broader printable functional feature sizes ranging from microns to centimeters, providing a viable and reliable technical solution for tissue and organ printing in the future.

3D bioprinting↗