Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “CELL DIVISION”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 811 records · Page 45
All sourcesNASA NTRSDOE OSTI
DOE OSTI2016

Warthog: Progress on Coupling BISON and PROTEUS

The Nuclear Energy Advanced Modeling and Simulation (NEAMS) program from the Office of Nuclear Energy at the Department of Energy (DOE) provides a robust toolkit for modeling and simulation of current and future advanced nuclear reactor designs. This toolkit provides these technologies organized across product lines, with two divisions targeted at fuels and end-to-end reactor modeling, and a third for integration, coupling, and high-level workflow management. The Fuels Product Line (FPL) and the Reactor Product Line (RPL) provide advanced computational technologies that serve each respective field effectively. There is currently a lack of integration between the product lines, impeding future improvements of simulation solution fidelity. In order to mix and match tools across the product lines, a new application called Warthog was produced. Warthog is built on the Multi-physics Object-Oriented Simulation Environment (MOOSE) framework developed at Idaho National Laboratory (INL). This report details the continuing efforts to provide the Integration Product Line (IPL) with interoperability using the Warthog code. Currently, this application strives to couple the BISON fuel performance application from the FPL using the PROTEUS Core Neutronics application from the RPL. Warthog leverages as much prior work from the NEAMS program as possible, enabling interoperability between the independently developed MOOSE and SHARP frameworks, and the libMesh and MOAB mesh data formats. Previous work performed on Warthog allowed it to couple a pin cell between the two codes. However, as the temperature changed due to the BISON calculation, the cross sections were not recalculated, leading to errors as the temperature got further away from the initial conditions. XSProc from the SCALE code suite was used to calculate the cross sections as needed. The remainder of this report discusses the changes to Warthog to allow for the implementation of XSProc as an external code. It also discusses the changes made to Warthog to allow it to fit more cleanly into the MultiApp syntax of the MOOSE framework. The capabilities, design, and limitations of Warthog will be described, in addition to some of the test cases that were used to demonstrate the code. Future plans for Warthog will be discussed, including continuation of the modifications to the input and coupling to other SHARP codes such as Nek5000.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗
DOE OSTI2026

Supervisory Control and Data Acquisition for Electrochemical Separation Experimentation

The Python-based program is a laboratory automation tool designed to control and monitor electrochemical systems. The tool was developed for capacitive deionization (CDI) experiments, but it can be used for any system that requires controlled voltage or current segments and multi-parameter monitoring. The program integrates hardware components to run user-defined experimental parameters, providing operational control of a programmable power supply, peristaltic pump, and data acquisition devices. Currently, the program is structured with a workflow that includes an initialization (or pre-run) phase, a main loop, and a post-experiment stabilization (or post-run) phase. The initialization phase prepares and stabilizes the cell, ensuring that the electrodes and solution reach a baseline state before the experiment begins. The main loop consists of multiple voltage segments that repeat, controlling the experiment while recording key parameters such as time, voltage, current, pH, and conductivity. Finally, the post-experiment stabilization phase allows the system to stabilize after the experiment, returning the cell and solution to equilibrium conditions before ending the sequence. The program is designed with four variations, each tailored to different experimental needs. All variations include both the initialization and post-experiment stabilization stages, which run for a set amount of time, voltage, current, and flow rate before and after the main experiment block. The main loop runs for a set number of cycles, as defined by the user input, and each cycle is composed of 2 or 4 segments. The 4 program variations are described as follows: Program 1: The main program includes 2 segments. Each segment is defined to have a set duration, flow rate, voltage, and current. This program measures conductivity, flow rate, voltage, and current. Program 2: The main program expands Program 1 to include 4 segments. Each segment has a specified duration, flow rate, voltage, and current. Like Program 1, it measures conductivity, flow rate, voltage, and current. Program 3: The main program consists of 2 segments, each defined by time, flow rate, voltage, and current. In addition to conductivity, flow rate, voltage, and current, Program 3 collects pH and temperature data through a 4-channel data acquisition device. Program 4: This program independently controls two channels of a multi-channel power supply simultaneously. While conductivity can only be measured for one cell at a time, the dual-channel control makes it possible to operate two cells simultaneously under different voltage/current conditions. The main program includes 2 segments.For each program, all measurements are automatically logged and integrated into a single Excel output file. Data are displayed in numerical format and plotted, both in real time, to track system performance. A key feature of the program is its ability to synchronize all outputs so that every measurement shares a single timestamp, ensuring accurate alignment of voltage, current, pH, conductivity, and pH data.By combining hardware control, real-time monitoring, and unified data collection, this program significantly reduces manual workload and minimizes errors, making it a reliable platform for researchers, engineers, and laboratory technicians conducting CDI experiments, among other electrochemical tests.

Valentino, Lauren [Argonne National Laboratory (AN↗
DOE OSTI2026

Human Coronavirus 229E Infection Alters Histone Proteoforms

Viruses rely on host machinery to replicate, and growing evidence demonstrates that they utilize host epigenetic regulation, including histone modification, to modulate host gene expression for their benefit. Herein, we employed top-down proteomics to quantify histone proteoforms in a model human lung cell line following human coronavirus 229E (HCoV-229E) infection and compared them to mock-infected controls. A total of 572 proteoforms from mock-infected and HCoV-229E infected human lung fibroblast (MRC5) cells (N = 5 per condition) were identified; this included 461 histone proteoforms that were assigned to H2A, H2B, H3, or H4. 200 histone proteoforms were quantifiable, and differential abundance analysis revealed several statistically significant changes in both reversible post-translational modifications (e.g. phosphorylation, acetylation) and the truncation states of core histones. Notably, we found decreased abundance of C-terminally truncated histone H2A and N-terminally truncated histone H3 in HCoV-229E-infected samples. These findings underscore the power of top-down proteomics to resolve unique truncation states of proteoforms and support the hypothesis that viruses alter histone length (removing regulatory sites) to influence host gene expression.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2026

All-organic self-separating three-dimensionally nanoarchitected electrochemical energy storage devices

This work realizes a three-dimensionally (3D) nanoarchitected, all organic, "self-separating" lithium-ion electrochemical energy storage (EES) device that is cycled as a solid-state full cell. The device is enabled by a monolithic carbon anode with a co-continuous pore network, derived from the structure direction of resols by an ultra-large molar mass block copolymer (BCP), poly(styrene-block-2-dimethylaminoethyl methacrylate) (SA). Electropolymerization of a single-phase conductive and redox-active material, poly((2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methyl 9,10-dioxo-9,10-dihydroanthracene-2-carboxylate) (PAQEDOT), into the pore space provides the cathode of the cell. The device is electronically contacted to the relevant electrode network enabled by the co-continuous nature of each electrode. Electrochemical processing via cycling against external lithium in an electrolyte generates a solid electrolyte interphase (SEI) as a separator and lithiates the cell electrodes, after which the EES device is cycled in the solid state. While the full cell does not demonstrate high cyclability, the best full cell demonstrates a discharge capacity of 267 milliamp hours per gram (mAh/g). This work marks, to the best of knowledge of the authors, the first example of an all-organic materials derived 3D nanoarchitected EES device, as well as the first design of "self-separating" cell fabrication. Furthermore, generalization of the design to another co-continuous carbon form factor is demonstrated.

FOS: Physical sciences↗
NASA NTRS2003

NASA's Bioreactor: Growing Cells in a Simulated Microgravity Environment

National Science Education Standards (NSES), Science for All Americans, the Secretary's Commission on Achieving Necessary Skills (SCANS) as well as the National Aeronautics and Space Administration (NASA) are all making an effort to promote scientific literacy in America. Unfortunately, major evaluation programs such as the National Assessment of Educational Progress (NAEP) and the Third International Mathematics and Science Study (TIMSS) have provided information that suggested our students are not able to compete with peers from comparable countries. Although results indicated that American students are recalling memorized, factual knowledge well enough, the real problem is the ability to apply what they know. Concerned with these reports, the National Science Teacher's Association (NSTA) has developed a mission to support innovation and high quality in science teaching and learning for every student. NSTA recommends less emphasis on factual knowledge (memorization) and information and more understanding of the concepts. Science process skills are considered imperative to prepare America's students for the 21st century. The National Aeronautics and Space Administration (NASA) supports this mission and adds that NASA strives to help prepare and encourage the next generation of researchers and explorers. One method that NASA supports educators and its mission is to publish educational briefs. NASA describes a brief as a publication that ranges from one-to-thirty pages. The focus is on mission discoveries and results. The brief provides curriculum to educators that supports their objectives and NASA's interest. Educational Briefs are specific to the grade level and course so that educators may have choices that fit their methods and students level. Sometimes, the brief includes lessons and activities teachers may use. For example, NASA's Microgravity Division has designed a student bioreactor. Consequently, an Educational Brief is being written that focuses on how to build a student bioreactor and experiments that can be conducted in it. These experiments mimic the experiments done by NASA and other researchers in the real world of work.

Richardson, Denise↗
DOE OSTI2018

The Michigan New Technology Training and Research (MNTR) Translational Program in Nuclear Medicine (Final Report)

The Michigan New Technology Training and Research (MNTR) Translational Program in Nuclear Medicine and Molecular Imaging This project established the Michigan New Technology Training and Research (MNTR) translational program, focused on the acceleration of new clinical molecular imaging methods. Molecular imaging approaches to imaging are a very flexible and powerful development, with many recent examples advancing the diagnosis and guiding the management of human disease. These methods depend on the design, synthesis and testing of new radiotracers (molecules designed to target specific cells or tissues in the body), labeled with short-lived radioactive atoms (positron emitters). The new radiotracers are imaged in the human body using positron emission tomography (PET) imaging. The University of Michigan PET Center has considerable experience in the discovery, development, refinement and initial human applications of new PET radiotracers. An emerging impediment to efficient availability of new PET radiotracers, however, involves the regulatory environment at multiple levels. Many potentially successful ideas are delayed or even prevented from entering human use by these barriers. In the current grant, we provided hands-on experience for the next generation of young scientists working in molecular imaging so that they are equipped to effectively move new ideas and radiotracers from the research laboratory to the nuclear medicine clinic (bench-to-bedside). For new radiotracer development, oversight by the Food and Drug Administration (FDA), the Nuclear Regulatory Commission (NRC), and local institutional committees and reviews are required. These aspects of translation from laboratory to clinic are often omitted from the formal training of new scientists, but were provided to three trainees via direct mentorship in the MNTR program. The MNTR training program built on the translational successes of the University of Michigan Division of Nuclear Medicine and capitalized on our strengths (facilities, faculty, a wide-ranging selection of projects, and the experience and commitment to bring new radiotracers into human studies) to systematically train three fellows in radiochemistry and nuclear medicine. Integrated training in development, validation and clinical translation of new PET radiotracers occurred through practical involvement in multiple research projects, each at a different stage along the pathway from bench-to-bedside. Each trainee obtained experience of the entire process over about 2 years. Projects involved development of new radiotracers for neurodegenerative disorders (e.g. Alzheimer disease), movement disorders (e.g. Parkinson disease, progressive supranuclear palsy) and neurodevelopmental syndromes (e.g. Tourette syndrome) as well as new radiochemistry methods for synthesizing radiotracers. Each project was pursued in a close collaboration between the fellow trainee and both the clinical and basic science faculty at the University of Michigan PET Center, and provided an unprecedented opportunity for the highest-level training in academic radiochemistry and nuclear medicine research. The project has resulted in 17 peer-reviewed publications to date, and at least 4 more are in preparation. Additionally, 1 book chapter and 26 conference presentations have resulted from this grant. Upon completion of the MNTR Program, the trainees have emerged as translational scientists wielding the necessary skill-set (practical research experience, multidisciplinary training, regulatory know-how) to embark on successful careers in translational molecular imaging, bringing continued advances in the field to bear on important human disorders. As a metric of the Program’s success, one fellow has already secured employment in the field, while the other two are finishing writing papers; they wish to also stay in the radiochemistry and nuclear medicine community, and are currently applying for jobs.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗
NASA NTRS2015

Resource Prospector (RP) - Early Prototyping and Development

The Resource Prospector (RP) is an In-Situ Resource Utilization (ISRU) technology demonstration mission under study by the NASA Human Exploration and Operations Mission Directorate's (HEOMD) Advanced Exploration Systems (AES) Division. The mission, currently planned to launch in 2020, will demonstrate extraction of oxygen from lunar regolith to validate ISRU capability. The mission will address key Strategic Knowledge Gaps (SKGs) for robotic and human exploration to the Moon, Near Earth Asteroids (NEAs), and ultimately Mars, as well as meet the strategic goals of the Global Exploration Roadmap (GER), offered by the International Space Exploration Coordination Group (ISECG). In this roadmap, the use of local resources is specifically addressed relating to human exploration. RP will provide knowledge to inform the selection of future mission destinations, support the development of exploration systems, and reduce the risk associated with human exploration. Expanding human presence beyond low-Earth orbit to asteroids and Mars will require the maximum possible use of local materials, so-called in-situ resources. The moon presents a unique destination to conduct robotic investigations that advance ISRU capabilities, as well as providing significant exploration and science value. Lunar regolith contains useful resources such as oxygen, water, silicon, and light metals, like aluminum and titanium. Oxygen can be separated from the regolith for life support (breathable air), or used to create rocket propellant (oxidizer). Regolith can be used to protect against radiation exposure, be processed into solar cells, or used to manufacture construction materials such as bricks and glass. RP will characterize the constituents and distribution of water and other volatiles at the poles of the Moon, enabling innovative uses of local resources, in addition to validating ISRU capabilities. This capability, as well as a deeper understanding of regolith, will be valuable in the exploration of near-Earth asteroids (NEAs) and Mars. In order to reduce risk and explore system designs, the RP project is attempting two-fold approaches to development as it looks towards flight. We continue to explore flight planning, requirements, and interfaces definition by using Engineering Test Units (ETUs), looking towards lunar deployment, while also using fiscal year 2015 to develop, build and test an earth-terrestrial prototype rover and payload system. This terrestrial prototype, called "RP15", is built to both inform the system design, and to be a partnership advocacy tool for this unique mission. RP15 must be affordable within the resource and time constraints of fiscal year 2015, while working to the following Needs, Goals, and Objectives provided by HEOMD/AES: 1. Demonstrate rover mobility in a 1g environment 2. The Surface Segment (prototype rover + payload system) shall represent the flight system concept with as much fidelity as affordable (limited by cost and schedule) - Surface Segment shall be the approximate size/dimension/footprint -Surface Segment shall package all the expected devices (instruments, systems, etc.), even if some facets are mocked-up due to time/cost constraints -Overall Surface Segment fidelity negotiable to make achievable 3. Priority should be given to illustrating mission functionality over support functionality, which exists solely to support mission functionality This paper will provide an overview of RP project developments, including the design and build, capturing the development and initial integrated testing of RP15 in relevant environments.

Lunar↗
DOE OSTI2012

Synthesis, Characterization, and Reactivity of Fe Complexes Containing Cyclic Diazadiphosphine Ligands: The Role of the Pendant Base in Heterolytic Cleavage of H 2

The iron complexes CpFe(PPh2NBn2)Cl (1-Cl), CpFe(PPh2NPh2)Cl (2-Cl), CpFe(PPh2C5)Cl (3-Cl) (where PPh2NBn2 is 1,5-dibenzyl-1,5-diaza-3,7-diphenyl-3,7-diphosphacyclooctane, PPh2NPh2 is 1,3,5,7-tetraphenyl-1,5-diaza-3,7-diphosphacyclooctane, and PPh2C5 is 1,4-diphenyl-1,4-diphosphacycloheptane) have been synthesized and characterized by NMR spectroscopy, electrochemical studies, and X-ray diffraction studies. These chloride derivatives are readily converted to the corresponding hydride complexes CpFe(PPh2NBn2)H (1-H), CpFe(PPh2NPh2)H (2-H), CpFe(PPh2C5)H (3-H)] and H2 complexes [CpFe(PPh2NBn2)(H2)]BArF4, [1-H2]BArF4, (where BArF4 is B[(3,5-(CF3)2C6H3)4]-), [CpFe(PPh2NPh2)(H2)]BArF4, [2-H2]BArF4, and [CpFe(PPh2C5)(H2)]BArF4, [3-H2]BArF4 as well as [CpFe(PPh2NBn2)(CO)]BArF4, [1-CO]BArF4. Structural studies are reported for [1-H2]BArF4, 1-H, 2-H, and [1-CO]BArF4. The conformations adopted by the chelate rings of the PPh2NBn2 ligand in the different complexes are determined by attractive or repulsive interactions between the sixth ligand of these pseudo-octahedral complexes and the pendant N atom of the ring adjacent to the sixth ligand. An example of an attractive interaction is the observation that the distance between the N atom of the pendant amine and the C atom of the coordinated CO ligand for [1-CO]BArF4 is 2.848 Å, considerably shorter than the sum of the van der Waals radii of N and C atoms. Experimental and theoretical studies of H/D exchange by the complexes [1-H2]+, [2-H2]+, and [3-H2]+ indicate that the relatively rapid exchange observed for [1-H2]+ and [2-H2]+ compared to [3-H2]+ is consistent with intramolecular heterolytic cleavage of H2 mediated by the pendant amine. These mononuclear FeII dihydrogen complexes containing pendant amines in the ligands mimic crucial features of the distal Fe site of the active site of the [FeFe] hydrogenase required for H-H bond formation and cleavage. We thank the U.S. Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences, Biosciences and Geosciences, for support of this research. S.C. (DFT computations) and M. J. O. (NMR experiments) were supported by the Center for Molecular Electrocatalysis, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under FWP 56073. Pacific Northwest National Laboratory is operated by Battelle for the U.S. Department of Energy.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Four-Dimensional Quasielastic Neutron Scattering (4D-QENS) Analysis of Correlated Ionic Conduction in Sr⁢Cl2

Methods of elucidating the mechanisms of fast-ion conduction in solid-state materials are pivotal for advancements in energy technologies such as batteries, fuel cells, sensors, and supercapacitors. In this study, we examine the ionic conduction pathways in single crystal Sr⁢Cl2, which is a fast-ion conductor above 900 K, using four-dimensional quasielastic neutron scattering (4D-QENS). We explore both coherent and incoherent neutron scattering at temperatures above the transition temperature into the superionic phase to explore the correlated motion of hopping anions. Refinements of the incoherent QENS yield residence times and jump probabilities between lattice sites in good agreement with previous studies, confirming that ionic hopping along nearest-neighbor directions is the most probable conduction pathway. However, the coherent QENS reveals evidence of de Gennes narrowing, indicating the importance of ionic correlations in the conduction mechanism. This highlights the need for improvements both in the theory of ionic transport in fluorite compounds and the modeling of coherent 4D-QENS in single crystals.

Coles, Jared [Materials Science Division, Argonne ↗
DOE OSTI2013

Development of a Low Cost 10kW Tubular SOFC Power System

The DOE program funded from 2003 through early 2013 has brought the Acumentrics SOFC program from an early stage R&D program to an entry level commercial product offering. The development work started as one of the main core teams under the DOE Solid State Energy Conversion Alliance (SECA) program administered by the National Energy Technology Laboratory (NETL) of the DOE. During the first phase of the program, lasting approximately 3-4 years, a 5kW machine was designed, manufactured and tested against the specification developed by NETL. This unit was also shipped to NETL for independent verification testing which validated all of the results achieved while in the laboratory at Acumentrics. The Acumentrics unit passed all criteria established from operational stability, efficiency, and cost projections. Passing of the SECA Phase I test allowed the program to move into Phase II of the program. During this phase, the overall objective was to further refine the unit meeting a higher level of performance stability as well as further cost reductions. During the first year of this new phase, the NETL SECA program was refocused towards larger size units and operation on coal gasification due to the severe rise in natural gas prices and refocus on the US supply of indigenous coal. At this point, the program was shifted to the U.S. DOE’s Energy Efficiency and Renewable Energy (EERE) division located in Golden, Colorado. With this shift, the focus remained on smaller power units operational on gaseous fuels for a variety of applications including micro combined heat and power (mCHP). To achieve this goal, further enhancements in power, life expectancy and reductions in cost were necessary. The past 5 years have achieved these goals with machines that can now achieve over 40% electrical efficiency and field units that have now operated for close to a year and a half with minimal maintenance. The following report details not only the first phase while under the SECA program and the key achievements but also the results while under EERE’s leadership and the transition to an early commercial product offering.

10 SYNTHETIC FUELS↗
DOE OSTI2012

Active Hydrogenation Catalyst with a Structured, Peptide-Based Outer-Coordination Sphere

The synthesis, catalytic activity, and structural features of a rhodium-based hydrogenation catalyst containing a phosphine ligand coupled to a 14-residue peptide are reported. Both CD and NMR spectroscopy show that the peptide adopts a helical structure in 1:1:1 TFE/MeCN/H2O that is maintained when the peptide is attached to the ligand and when the ligand is attached to the metal complex. The metal complex hydrogenates aqueous solutions of 3-butenol to 1-butanol at 360 ± 50 turnovers/Rh/h at 294 K. This peptide- based catalyst represents a starting point for developing and characterizing a peptide-based outer-coordination sphere that can be used to introduce enzyme-like features into molecular catalysts. This work was funded by the US DOE Basic Energy Sciences, Chemical Sciences, Geoscience and Biosciences Division (AJ, JCL and WJS), the Office of Science Early Career Research Program through the Office of Basic Energy Sciences (GWB, MLR and WJS). Part of the research was conducted at the W.R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by U.S. Department of Energy’s Office of Biolog-ical and Environmental Research (BER) program located at Pacific Northwest National Laboratory (PNNL). PNNL is operated by Battelle for the U.S. Department of Energy.

amino acid catalysts↗
NASA NTRS1999

Characterization of Infrared Diode Laser Beams and Atmospheric CO Imaging Instrument

During June-August 1997 Dr. Jonathan Miles participated in the ASEE-sponsored summer faculty research program at NASA Langley Research Center (LaRC). The Aerospace Electronic Systems Division (AESD), Sensor Systems Branch (SSB), at NASA LARC had proposed a new mission, GEOstationary TROpospheric Pollution SATellite (GEO TROPSAT), to address critical science questions of tropospheric chemistry. The troposphere is a complex system, comprising "point" and distributed sources of natural and anthropogenic origin; complicated transport processes, both lateral and vertical; and photochemistry driven by UV flux, temperature, atmospheric composition, and other variables. GEO TROPSAT would be implemented about a geostationary Earth orbital (GEO) position at the equator between 600 and 80" West longitude to observe the Americas and large portions of the oceans of either coast. This mission would advance our knowledge of the atmosphere by capturing the wide temporal and spatial variability of tropospheric phenomena which is undetectable from low Earth orbit. A pre-prototype imaging carbon monoxide (CO) imaging system operating within a narrow waveband about 4.7 [Lm was built, demonstrated, and evaluated. This system applies the gas-filter correlation radiometry (GFCR) technique and produces digitized images comprising 4096 pixels, each representing a single CO mixing ratio measurement inferred from radiometric data. Associated tasks accomplished included specification for the next-generation prototype system to operate in the 2.3-@tm waveband; characterization of a 64x64, InSb focal-plane-array (FPA) imager; design, fabrication, and assembly of a filter wheel; and software development. Laboratory evaluation of this system involved imaging of a test cell placed in the path of radiant flux emanating from a blackbody source used to simulate the radiant energy reflected by Earth in real application. The cell was evacuated for system balancing and then charged with measured quantities of CO to provide a means for system characterization. Two related research efforts were undertaken during the term of the study reported here - continued development of an atmospheric CO imaging instrument and characterization of diode-laser beams. Both efforts were successful and are described within the body of this report. A second objective was to provide a means for undergraduate ISAT majors to become involved with the research described, to be afforded the opportunity to learn the technologies associated with the work performed. Two ISAT students gained a comprehensive understanding and interest in missions supported by NASA through direct involvement in this project. Many more students were exposed to these technologies through demonstrations, laboratory tours, and explanations provided in lectures.

Miles, Jonathan J.↗
DOE OSTI2014

Daymet: Daily Surface Weather Data on a 1-km Grid for North America, Version 2.

More information: http://daymet.ornl.gov Presenter: Ranjeet Devarakonda Environmental Sciences Division Oak Ridge National Laboratory (ORNL) Daymet: Daily Surface Weather Data and Climatological Summaries provides gridded estimates of daily weather parameters for North America, including daily continuous surfaces of minimum and maximum temperature, precipitation occurrence and amount, humidity, shortwave radiation, snow water equivalent, and day length. The current data product (Version 2) covers the period January 1, 1980 to December 31, 2013 [1]. The prior product (Version 1) only covered from 1980-2008. Data are available on a daily time step at a 1-km x 1-km spatial resolution in Lambert Conformal Conic projection with a spatial extent that covers the conterminous United States, Mexico, and Southern Canada as meteorological station density allows. Daymet data can be downloaded from 1) the ORNL Distributed Active Archive Center (DAAC) search and order tools (http://daac.ornl.gov/cgi-bin/cart/add2cart.pl?add=1219) or directly from the DAAC FTP site (http://daac.ornl.gov/cgi-bin/dsviewer.pl?ds_id=1219) and 2) the Single Pixel Tool [2] and THREDDS (Thematic Real-time Environmental Data Services) Data Server [3]. The Single Pixel Data Extraction Tool allows users to enter a single geographic point by latitude and longitude in decimal degrees. A routine is executed that translates the (lon, lat) coordinates into projected Daymet (x,y) coordinates. These coordinates are used to access the Daymet database of daily-interpolated surface weather variables. Daily data from the nearest 1 km x 1 km Daymet grid cell are extracted from the database and formatted as a table with one column for each Daymet variable and one row for each day. All daily data for selected years are returned as a single (long) table, formatted for display in the browser window. At the top of this table is a link to the same data in a simple comma-separated text format, suitable for import into a spreadsheet or other data analysis software. The Single Pixel Data Extraction Tool also provides the option to download multiple coordinates programmatically. A multiple extractor script is freely available to download at http://daymet.ornl.gov/files/daymet.zip. The ORNL DAAC s THREDDS data server (TDS) provides customized visualization and access to Daymet time series of North American mosaics. Users can subset and download Daymet data via a variety of community standards, including OPeNDAP, NetCDF Subset service, and Open Geospatial Consortium (OGC) Web Map/Coverage Service. The ORNL DAAC TDS also exposes Daymet metadata through its ncISO service to facilitate harvesting Daymet metadata records into 3rd party catalogs. References: [1] Thornton, P.E., M.M. Thornton, B.W. Mayer, N. Wilhelmi, Y. Wei, R. Devarakonda, and R.B. Cook. 2014. Daymet: Daily Surface Weather Data on a 1-km Grid for North America, Version 2. Data set. Available on-line [http://daac.ornl.gov] from Oak Ridge National Laboratory Distributed Active Archive Center, Oak Ridge, Tennessee, USA. [2] Devarakonda R., et al. 2012. Daymet: Single Pixel Data Extraction Tool. Available on-line [http://daymet.ornl.go/singlepixel.html]. [3] Wei Y., et al. 2014. Daymet: Thematic Real-time Environmental Data Services. Available on-line [http://daymet.ornl.gov/thredds_tiles.html].

Thornton, Peter E↗
NASA NTRS1992

Selected OAST/OSSA space experiment activities in support of Space Station Freedom

The Space Experiments Division at NASA Lewis Research Center is developing technology and science space experiments for the Office of Aeronautics and Space Technology (OAST) and the Office of Space Sciences and Applications (OSSA). Selected precursor experiments and technology development activities supporting the Space Station Freedom (SSF) are presented. The Tank Pressure Control Experiment (TPCE) is an OAST-funded cryogenic fluid dynamics experiment, the objective of which is to determine the effectiveness of jet mixing as a means of equilibrating fluid temperatures and controlling tank pressures, thereby permitting the design of lighter cryogenic tanks. The information from experiments such as this will be utilized in the design and operation of on board cryogenic storage for programs such as SSF. The Thermal Energy Storage Flight Project (TES) is an OAST-funded thermal management experiment involving phase change materials for thermal energy storage. The objective of this project is to develop and fly in-space experiments to characterize void shape and location in phase change materials used in a thermal energy storage configuration representative of an advanced solar dynamic system design. The information from experiments such as this will be utilized in the design of future solar dynamic power systems. The Solar Array Module Plasma Interaction Experiment (SAMPIE) is an OAST-funded experiment to determine the environmental effects of the low earth orbit (LEO) space plasma environment on state-of-the-art solar cell modules biased to high potentials relative to the plasma. Future spacecraft designs and structures will push the operating limits of solar cell arrays and other high voltage systems. SAMPIE will provide key information necessary for optimum module design and construction. The Vibration Isolation Technology (VIT) Advanced Technology Development effort is funded by OSSA to provide technology necessary to maintain a stable microgravity environment for sensitive payloads on board spacecraft. The proof of concept will be demonstrated by laboratory tests and in low-gravity aircraft flights. VIT is expected to be utilized by many SSF microgravity science payloads. The Space Acceleration Measurement System (SAMS) is an OSSA-funded instrument to measure the microgravity acceleration environment for OSSA payloads on the shuttle and SSF.

Delombard, Richard↗
NASA NTRS2017

Novel Approach to Quantification of Telomere Length with Direct Nanopore Sequencing and PCR Amplification

The ends of human chromosomes contain telomeres, or tandem arrays of repeating DNA sequences capped by multiple associated proteins that protect chromosomal ends from degradation. Telomeres function to preserve genomic stability by preventing natural chromosomal ends from being recognized as broken DNA double-strand breaks and triggering inappropriate DNA damage responses. Mounting evidence shows telomere length is an inherited trait that decreases with cellular division and normal aging. In addition, telomere length also appears to be influenced by other factors such as cellular oxidative stress, radiation and mechanical unloading of tissues as in microgravity. To measure these potential effects of the space environment on telomere lengths and cellular aging and regenerative potential we developed a novel telomere measurement approach based on nanopore sequencing of PCR amplified bar-coded chromosome termini. Specifically, telomeres can be directly enriched using barcode sequences ligated to the end of a free end- repaired telomere using the WetLab-2 facility SmartCycler on ISS. Prior to the ligation and amplification protocol a proteinase K digestion of capping proteins followed by a single 95-degree C heat denaturation of the protease is included. After digestion and bar-code ligation, PCR amplification will initiate with the ligated barcoded sequence, suppressing amplification of intra-genomic fragments and resulting in long read barcoded telomere amplicons including the nanopore motor protein sequences. Purified PCR amplicons are then used for nanopore sequencing library generation by simple addition of motor proteins and sequencing library is loaded into the MinION nanopore DNA-sequencer. Amplicon sequence reads from the nanopore device can be base-called quickly on ISS due to barcoding ligation and subsequent PCR amplification enhancing the telomere sequence resolution. If successfully implemented on ISS this technique will provide a novel means of measuring regenerative ability of somatic stem cells in astronauts, and of determining whether spaceflight in microgravity alters their telomere lengths and causes premature cellular aging.

Ma, Kristin R.↗