Results for “sequencing test” — Page 44

NASA NTRS2010

Comparison of Airborne and Ground-Based Function Allocation Concepts for NextGen Using Human-In-The-Loop Simulations

This paper presents an air/ground functional allocation experiment conducted by the National Aeronautics and Space Administration (NASA) using two human-in-the-Loop simulations to compare airborne and ground-based approaches to NextGen separation assurance. The approaches under investigation are two trajectory-based four-dimensional (4D) concepts; one referred to as "airborne trajectory management with self-separation" (airborne) the other as "ground-based automated separation assurance" (ground-based). In coordinated simulations at NASA's Ames and Langley Research Centers, the primary operational participants -controllers for the ground-based concept and pilots for the airborne concept - manage the same traffic scenario using the two different 4D concepts. The common scenarios are anchored in traffic problems that require a significant increase in airspace capacity - on average, double, and in some local areas, close to 250% over current day levels - in order to enable aircraft to safely and efficiently traverse the test airspace. The simulations vary common independent variables such as traffic density, sequencing and scheduling constraints, and timing of trajectory change events. A set of common metrics is collected to enable a direct comparison of relevant results. The simulations will be conducted in spring 2010. If accepted, this paper will be the first publication of the experimental approach and early results. An initial comparison of safety and efficiency as well as operator acceptability under the two concepts is expected.

Wing, David J.↗

NASA NTRS2019

NEXT Ion Propulsion System Risk Mitigation Tests in Support of the Double Asteroid Redirection Test Mission

Risk mitigation tests have been conducted by the NASA Glenn Research Center and The Aerospace Corporation in support of the DART Mission. The tests focused on NEXT performance characterizations intended to ensure its operations and characteristics are compatible with the DART mission operations, and to assist in the definition of the propulsion system. Tests were performed at the Aerospace Corporation and they involved: flow sensitivity-analyses, steady-state performance characterizations, and measurements of thruster erosion. The tests also involved defining, demonstrating, verifying, and evaluating the start-up sequences and a beam current regulation algorithm consistent with DART mission requirements. It was found that NEXT thruster operations are compatible with the proposed relaxation of flow control ranges for ignition and for steady-state operation.

Thomas, Robert E.↗

NASA NTRS2019

NEXT Ion Propulsion System Risk Mitigation Tests in Support of the Double Asteroid Redirection Test Mission

Risk mitigation tests have been conducted by the NASA Glenn Research Center and The Aerospace Corporation in support of the DART Mission. The tests focused on NEXT performance characterizations intended to ensure its operations and characteristics are compatible with the DART mission operations, and to assist in the definition of the propulsion system. Tests were performed at the Aerospace Corporation and they involved: flow sensitivity-analyses, steady-state performance characterizations, and measurements of thruster erosion. The tests also involved defining, demonstrating, verifying, and evaluating the start-up sequences and a beam current regulation algorithm consistent with DART mission requirements. It was found that NEXT thruster operations are compatible with the proposed relaxation of flow control ranges for ignition and for steady-state operation.

Thomas, Robert E.↗

NASA NTRS2019

NEXT Ion Propulsion System Risk Mitigation Tests in Support of the Double Asteroid Redirection Test Mission

Risk mitigation tests have been conducted by the NASA Glenn Research Center and The Aerospace Corporation in support of the DART Mission. The tests focused on NEXT performance characterizations intended to ensure its operations and characteristics are compatible with the DART mission operations, and to assist in the definition of the propulsion system. Tests were performed at the Aerospace Corporation and they involved: flow sensitivity-analyses, steady-state performance characterizations, and measurements of thruster erosion. The tests also involved defining, demonstrating, verifying, and evaluating the start-up sequences and a beam current regulation algorithm consistent with DART mission requirements. It was found that NEXT thruster operations are compatible with the proposed relaxation of flow control ranges for ignition and for steady-state operation.

Thomas, Robert E.↗

NASA NTRS2009

Roadmaps and Strategies for Crop Research for Bioregenerative Life Support Systems A Compilation of Findings from NASA’s Advanced Life Support Meetings

Extensive testing with plants for bioregenerative life support systems has been conducted by NASA for nearly 40 years, both through university grants and work at NASA field centers. During this time, numerous meetings were held to develop strategies and roadmaps for implementing bioregenerative life support systems. Findings from several of these meetings are summarized in this report. In nearly all cases, the recommendations included an active ground-based research program and integration tests with other life support subsystems, followed by eventual spaceflight testing. The most recent roadmap of NASA’s Exploration Life Support Crop Element suggests a sequence of first developing a modular vegetable production unit (VPU) to provide perishable foods to supplement the crew’s diet. A similar system could then be used on the lunar surface, with additional modules added as the lunar outpost expands. Eventually, these modules could be transferred to a dedicated pressurized logistics module (PLM) for crop production. Experience from the lunar tests could then be used to conduct a similar buildup for Mars missions, where in situ food production and bioregeneration would be important for achieving outpost autonomy. To date, most trade studies of bioregenerative life support systems indicate that reducing the costs associated with crop lighting systems are key, and use of light-emitting diodes (LEDs) or direct solar lighting offers promising options. Likewise, sustaining high yields and high harvest index (% edible biomass) will be important for minimizing costs, and this can be achieved by optimizing growth environments and through selecting, breeding, and genetically engineering crops suitable for space environments. Collectively, these strategies and roadmaps suggest that a combined approach of fundamental research, hardware (plant chamber) development, and operational testing is required to achieve reliable bioregenerative life support systems.

Bioregenerative↗

NASA NTRSDate not supplied

Design for Reliability (DfR) in Space Life Support

The engineering process of Design for Reliability (DfR) is well established in the automotive and aerospace industries. DfR should be useful in the future development of space life support systems. DfR is a sequence of tasks that develop system requirements and plan reliability analysis and testing. First and fundamentally, the reliability requirement is defined. Next the system reliability model is developed, often using a reliability block diagram. The overall system reliability requirement is allocated to the subsystems and an estimate of the attainable reliability is made. This expected reliability can be improved by simplifying the design by removing components or by replacing less reliable components. Improving reliability can require difficult compromises, such as reducing performance requirements, increasing budget, or extending testing. The actual system reliability can be determined only by testing, which should continue long enough to provide the required confidence in the measured value. New systems often have unexpected design errors that cause failures in early testing. The usual reliability improvement process of testing, finding the failure modes, and redesigning to remove them reduces the failure rate and is referred to as “reliability growth.” After redesign has been completed, the system should be further tested to determine the actual achieved reliability more accurately. If the final system failure rate is too high, redundant systems can be used to improve overall operational reliability. Adding redundancy simply to increase the one- or two-fault tolerance metric may sometimes reduce reliability. Reliability can be improved in three ways: redesigning the system to include more reliable subsystems and components, reliability growth testing and failure mode removal, and by using parallel redundant systems. DfR should combine these approaches to achieve the required reliability while managing performance, cost, and schedule.

Reliability↗

NASA NTRSDate not supplied

Design for Reliability (DfR) in Space Life Support

The engineering process of Design for Reliability (DfR) is well established in the automotive and aerospace industries. DfR should be useful in the future development of space life support systems. DfR is a sequence of tasks that develop system requirements and plan reliability analysis and testing. First and fundamentally, the reliability requirement is defined. Next the system reliability model is developed, often using a reliability block diagram. The overall system reliability requirement is allocated to the subsystems and an estimate of the attainable reliability is made. This expected reliability can be improved by simplifying the design by removing components or by replacing less reliable components. Improving reliability can require difficult compromises, such as reducing performance requirements, increasing budget, or extending testing. The actual system reliability can be determined only by testing, which should continue long enough to provide the required confidence in the measured value. New systems often have unexpected design errors that cause failures in early testing. The usual reliability improvement process of testing, finding the failure modes, and redesigning to remove them reduces the failure rate and is referred to as “reliability growth.” After redesign has been completed, the system should be further tested to determine the actual achieved reliability more accurately. If the final system failure rate is too high, redundant systems can be used to improve overall operational reliability. Adding redundancy simply to increase the one- or two-fault tolerance metric may sometimes reduce reliability. Reliability can be improved in three ways: redesigning the system to include more reliable subsystems and components, reliability growth testing and failure mode removal, and by using parallel redundant systems. DfR should combine these approaches to achieve the required reliability while managing performance, cost, and schedule.

Reliability↗

NASA NTRS1983

Load sequence effects on graphite/epoxy /+, -35/2s laminates

Load sequence effects on composite material fatigue behavior are due to the difference in residual strength when fatigue occurs, or the 'boundary effect', and the 'material money effect' due to previous loads. The present model, which allows the isolation of the effects of memory from those of boundary, has been investigated through a test program for the case of /+, -35/2s graphite/epoxy laminates under high-low and low-high load sequences which generated statistical residual strength data. As predicted by the model, the memory effect is present and a good correlation is obtained between test results and the theoretical predictions for both median residual strength and the expected percentage of fatigue failures under dual loading. It is noted, however, that the statistical dispersion of the residual strength test results is much smaller than that of the static ultimate strength.

Yang, J. N.↗

DOE OSTI2023

Insights from a workplace SARS-CoV-2 specimen collection program, with genomes placed into global sequence phylogeny

In 2020, the Department of Energy established the National Virtual Biotechnology Laboratory (NVBL) to address key challenges associated with COVID-19. As part of that effort, Pacific Northwest National Laboratory (PNNL) established a capability to collect and analyze specimens from employees who self-reported symptoms consistent with the disease. During the spring and fall of 2021, 688 specimens were screened for SARS-CoV-2, with 64 (9.3%) testing positive using reverse-transcriptase quantitative PCR (RT-qPCR). Of these, 36 samples were released for research. All 36 positive samples released for research were sequenced and genotyped. Here, the relationship between patient age and viral load as measured by Ct values was measured and determined to be only weakly significant. Consensus sequences for each sample were placed into a global phylogeny and transmission dynamics were investigated, revealing that the closest relative for many samples was from outside of Washington state, indicating mixing of viral pools within geographic regions.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

Whole genome resequencing reveals genomic regions associated with thermal adaptation in redband trout

Abstract Adaptation to local environments involves evolution of ecologically important traits and underlying physiological processes. Here, we used low coverage whole‐genome resequencing (lcWGR) on individuals to identify genome regions involved in thermal adaptation in wild redband trout Oncorhynchus mykiss gairdneri , a subspecies of rainbow trout that inhabits ecosystems ranging from cold montane forests to high elevation deserts. This study includes allele frequency‐based analyses for selective sweeps among populations, followed by multiple association tests for specific sets of phenotypes measured under thermal stress (acute and chronic survival/mortality; high or low cardiac performance groups). Depending on the groups in each set of analyses, sequencing reads covered 43%–75% of the genome at ≥15× and each analysis included millions of SNPs across the genome. In tests for selective sweeps among populations, a total of six chromosomal regions were significant. The further association tests for specific phenotypes revealed that the region on chromosome 4 was consistently the most significant and contains the cerk gene (ceramide kinase). This study provides insight into a potential genetic mechanism of local thermal adaptation and suggests c erk may be an important candidate gene. However, further validation of this cerk gene is necessary to determine if the association with cardiac performance results in a functional role to influence thermal performance when exposed to high water temperatures and hypoxic conditions.

Chen, Zhongqi↗

NASA NTRS2012

Testing of the NASA Hypersonics Project Combined Cycle Engine Large Scale Inlet Mode Transition Experiment (CCE LlMX)

Status on an effort to develop Turbine Based Combined Cycle (TBCC) propulsion is described. This propulsion technology can enable reliable and reusable space launch systems. TBCC propulsion offers improved performance and safety over rocket propulsion. The potential to realize aircraft-like operations and reduced maintenance are additional benefits. Among most the critical TBCC enabling technologies are: 1) mode transition from turbine to scramjet propulsion, 2) high Mach turbine engines and 3) TBCC integration. To address these TBCC challenges, the effort is centered on a propulsion mode transition experiment and includes analytical research. The test program, the Combined-Cycle Engine Large Scale Inlet Mode Transition Experiment (CCE LIMX), was conceived to integrate TBCC propulsion with proposed hypersonic vehicles. The goals address: (1) dual inlet operability and performance, (2) mode-transition sequences enabling a switch between turbine and scramjet flow paths, and (3) turbine engine transients during transition. Four test phases are planned from which a database can be used to both validate design and analysis codes and characterize operability and integration issues for TBCC propulsion. In this paper we discuss the research objectives, features of the CCE hardware and test plans, and status of the parametric inlet characterization testing which began in 2011. This effort is sponsored by the NASA Fundamental Aeronautics Hypersonics project

Saunders, J. D.↗

NASA NTRSDate not supplied

Validation of Image-Based Neural Network Controllersthrough Adaptive Stress Testing

Neural networks have become state-of-the-art for computer vision problems because of their ability to efficiently model complex functions from large amounts of data. While neural networks can be shown to perform well empirically fora variety of tasks, their performance is difficult to guarantee.Neural network verification tools have been developed that can certify robustness with respect to a given input image; however,for neural network systems used in closed-loop controllers,robustness with respect to individual images does not address multi-step properties of the neural network controller and itsenvironment. Furthermore, neural network systems interacting in the physical world and using natural images are operating in a black-box environment, making formal verification in-tractable. This work combines the adaptive stress testing (AST)framework with neural network verification tools to search for the most likely sequence of image disturbances that cause the neural network controlled system to reach a failure. Anautonomous aircraft taxi application is presented, and results show that the AST method finds failures with more likely image disturbances than baseline methods. Further analysis of AST results revealed an explainable cause of the failure, giving insight into the problematic scenarios that should be addressed.

Adaptive Stress Testing, Marabou, Deep Neural Netw↗

NASA NTRS1985

The influence of lay-up and thickness on composite impact damage and compression strength

The effects of composite stacking sequence, thickness, and percentage of zero-degree plies on the size, shape, and distribution of delamination through the laminate thickness and on residual compression strength following impact were studied. Graphite/epoxy laminates were impacted with an 0.5 inch diameter aluminum sphere at a specific low or high velocity. Impact damage was measured nondestructively by ultrasonic C-scans and X-radiography and destructively by the deply technique, and compression strength tests were performed. It was found that differences in compression failure strain due to stacking sequence were small, while laminates with very low percentages of zero-degree plies had similar failure loads but higher failure strains than laminates with higher percentages of zero-degree plies. Failure strain did not correlate with planar impact damage area, and delaminations in impact regions were associated with matrix cracking.

Guynn, E. G.↗

DOE OSTI2023

Impact of sanitizer application on Salmonella mitigation and microbiome shift on diced tomato during washing and storage

Here, this study examined the shift in Salmonella and indigenous microbiota on diced tomato after washing with three different sanitizers and during post-wash cold storage. Roma tomatoes were inoculated with a Salmonella cocktail (initial level ~ 5.6 CFU g –1 ), diced and washed along with uninoculated diced tomato in simulated flume wash water with sanitizers, including 10 mg L –1 free chlorine (FC10), 90 mg L –1 peracetic acid (PA90), PA90 in combination with a proprietary acidified surfactant blend (PS90), and unsanitized control (CK). Salmonella, total mesophilic aerobic bacteria (MAB), and yeast and mold (YM) populations on both inoculated and uninoculated samples were measured before and after washing, and during storage at 4 °C. 16S rRNA amplicon sequencing was also performed to determine the shift in bacterial microbiome. Washing with all tested sanitizers, especially PS90 (> 4 log Salmonella reduction), reduced Salmonella and MAB populations on inoculated diced tomatoes. Additionally, application of sanitizers mitigated Salmonella cross-contamination onto uninoculated samples. PS90 treatment inhibited the proliferation of most dominant bacteria on diced tomatoes during storage, including Erwiniaceae, Curtobacterium, Pantoea, Erwinia and Enterobacterales, which may benefit product quality and safety.

16S rRNA amplicon sequencing↗

NASA NTRS1998

A novel calmodulin-regulated Ca2+-ATPase (ACA2) from Arabidopsis with an N-terminal autoinhibitory domain

To study transporters involved in regulating intracellular Ca2+, we isolated a full-length cDNA encoding a Ca2+-ATPase from a model plant, Arabidopsis, and named it ACA2 (Arabidopsis Ca2+-ATPase, isoform 2). ACA2p is most similar to a "plasma membrane-type" Ca2+-ATPase, but is smaller (110 kDa), contains a unique N-terminal domain, and is missing a long C-terminal calmodulin-binding regulatory domain. In addition, ACA2p is localized to an endomembrane system and not the plasma membrane, as shown by aqueous-two phase fractionation of microsomal membranes. ACA2p was expressed in yeast as both a full-length protein (ACA2-1p) and an N-terminal truncation mutant (ACA2-2p; Delta residues 2-80). Only the truncation mutant restored the growth on Ca2+-depleted medium of a yeast mutant defective in both endogenous Ca2+ pumps, PMR1 and PMC1. Although basal Ca2+-ATPase activity of the full-length protein was low, it was stimulated 5-fold by calmodulin (50% activation around 30 nM). In contrast, the truncated pump was fully active and insensitive to calmodulin. A calmodulin-binding sequence was identified within the first 36 residues of the N-terminal domain, as shown by calmodulin gel overlays on fusion proteins. Thus, ACA2 encodes a novel calmodulin-regulated Ca2+-ATPase distinguished by a unique N-terminal regulatory domain and a non-plasma membrane localization.

Non-NASA Center↗

DOE OSTI2025

Sequential Stress Identifies Processing Defects in Bifacial Photovoltaic Modules That Limit Durability

Here, we use sequential stress to investigate hurdles to bifacial photovoltaic (PV) module durability from lamination defects. We test mini-modules with glass/glass (G/G) and glass/transparent-backsheet (G/TB) constructions using either ethylene vinyl acetate or polyolefin elastomer (POE) based encapsulants under a modified IEC 63209-2 sequential stress. This sequence includes multiple iterations of damp heat (DH200), full spectrum light exposure (A3), thermal cycling (TC50), and humidity/freeze (HF10). We compare indoor stress with outdoor exposure. Results show similar relative trends in degradation after a year outdoors compared to our first stress cycle. Subsequent stress cycles impart more severe damage than outdoor exposure for the short outdoor duration used here. Edge-pinch lamination defects in G/G mini-modules limit durability causing delamination and cell cracks. Conversely, we observe greater degradation in G/TB mini-modules compared to G/G in the later stages of the stress sequence when the backsheets are directly exposed to UV-containing light. Our results highlight: 1) the utility of sequential stress testing to uncover degradation modes in bifacial PV, 2) implications of using mini-modules for testing PV quality, and 3) the importance of lamination defects that must be avoided to ensure durability as the industry adopts G/G or G/TB packaging.

14 SOLAR ENERGY↗

NASA NTRS1983

Fracture behavior of hybrid composite laminates

The tensile fracture behavior of 15 center-notched hybrid laminates was studied. Three basic laminate groups were tested: (1) a baseline group with graphite/epoxy plies, (2) a group with the same stacking sequence but where the zero-deg plies were one or two plies of S-glass or Kevlar, and (3) a group with graphite plies but where the zero-deg plies were sandwiched between layers of perforated Mylar. Specimens were loaded linearly with time; load, far field strain, and crack opening displacement (COD) were monitored. The loading was stopped periodically and the notched region was radiographed to reveal the extent and type of damage (failure progression). Results of the tests showed that the hybrid laminates had higher fracture toughnesses than comparable all-graphite laminates. The higher fracture toughness was due primarily to the larger damage region at the ends of the slit; delamination and splitting lowered the stress concentration in the primary load-carrying plies. A linear elastic fracture analysis, which ignored delamination and splitting, underestimated the fracture toughness. For almost all of the laminates, the tests showed that the fracture toughness increased with crack length. The size of the damage region at the ends of the slit and COD measurements also increased with crack length.

Kennedy, J. M.↗

DOE OSTI2025

Design of Experiments for Dynamic Test Runs in Solvent-Based CO 2 Capture Pilot Plants

Test runs in the pilot plants consume significant resources, and therefore, the learning from test runs should be maximized. Test runs conducted in the pilot plants are often steady state. It takes several hours for reaching steady-state in the pilot plants, and thus, the duration of the test runs needs to be long even for collecting few steady-state data points. On the other hand, a large number of measurements can be collected through dynamic test runs in a short span of time. This paper presents a systematic design of dynamic experiments (DoDEs) for identifiability of model parameters, which is achieved by persistently exciting the inputs signals. A pseudorandom binary sequence (PRBS) is designed as the input signal for DoDE due to its efficiency in obtaining sufficient spectral content. However, due to the long sequence size of the PRBS signal, a Schroeder-phase input signal, which is a multisine signal, is also designed. Tests for both types of signals are run in the Pilot Solvent Test Unit (PSTU) at the National Carbon Capture Center in Wilsonville, Alabama. The transient data are used to solve dynamic data reconciliation and parameter estimation problem. The estimated parameters are found to be not only superior to those estimated from using data collected from hundreds of steady-state test runs in a nonreactive (air–water) system, but the parameters could be estimated by using the dynamic data collected for about 24 h from the pilot plant for the MEA-H 2 O–CO 2 system.

CO2 capture↗