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At least 793 records · Page 44

Cultural systems for growing potatoes in space

Higher plants are being evaluated for life support to provide needed food, oxygen and water as well as removal of carbon dioxide from the atmosphere. The successful utilization of plants in space will require the development of not only highly productive growing systems but also highly efficient bioregenerative systems. It will be necessary to recycle all inedible plant parts and all human wastes so that the entire complement of elemental compounds can be reused. Potatoes have been proposed as one of the desirable crops because they are 1) extremely productive, yielding more than 100 metric tons per hectare from field plantings, 2) the edible tubers are high in digestible starch (70%) and protein (10%) on a dry weight basis, 3) up to 80% of the total plant production is in tubers and thus edible, 4) the plants are easily propagated either from tubers or from tissue culture plantlets, 5) the tubers can be utilized with a minimum of processing, and 6) potatoes can be prepared in a variety of different forms for the human diet (Tibbitts et al., 1982). However potatoes have a growth pattern that complicates the development of growing the plants in controlled systems. Tubers are borne on underground stems that are botanically termed 'rhizomes', but in common usage termed 'stolons'. The stolons must be maintained in a dark, moist area with sufficient provision for enlargement of tubers. Stems rapidly terminate in flowers forcing extensive branching and spreading of plants so that individual plants will cover 0.2 m2 or more area. Thus the growing system must be developed to provide an area that is darkened for tuber and root growth and of sufficient size for plant spread. A system developed for growing potatoes, or any plants, in space will have certain requirements that must be met to make them a useful part of a life support system. The system must 1) be constructed of materials, and involve media, that can be reused for many successive cycles of plant growth, 2) involve a minimum quantity of media, 3) contain media that is essentially inert and not oxidize or degrade with use, 4) utilize a recirculating nutrient solution to permit regulation of pH and nutrient concentrations, and 5) be capable of complete automation of all planting, maintenance and harvesting procedures.

NASA Discipline Number 61-10↗

Plant Growth Research for Food Production: Development and Testing of Expandable Tuber Growth Module

Controlled and reliable growth of a variety of vegetable crops is an important capability for manned deep space exploration systems for providing nutritional supplementation and psychological benefits to crew members. Because current systems have been limited to leafy vegetables that require minimal root space, a major goal for these systems is to increase their ability to grow new types of crops, including tuber plants and root vegetables that require a large root space. An expandable root zone module and housing was developed to integrate this capability into the Veggie growth system. The expandable module uses a waterproof, gas-permeable bag with a structure that allows for root space to increase vertically throughout the growth cycle to accommodate for expanding tuber growth, while minimizing the required media mass. Daikon radishes were chosen as an ideal tuber crop for their subterraneous tuber size and rapid growth cycle, and investigations were done to study expanding superabsorbent hydrogels as a potential growth media. These studies showed improved water retention, but restricted oxygen availability to roots with pure gel media. It was determined that these hydrogels could be integrated in lower proportions into standard soil to achieve media expansion and water retention desired. Using the constructed module prototype and ideal gel and soil media mixture, Daikon radishes were grown in the system to test the capability and success of the system through a full growth cycle.

space↗

A Lunar Ground Truth of Microbes that are Integral to Sustaining Biological Life Support Systems

A lunar outpost such as Gateway or a Mars transit vehicle will use a semi-closed to closed loop biological life support system (BLSS) adapted for microgravity and high levels of radiation. A moon habitat will likely exist under partial gravity conditions and sustained levels of high radiation as a semi-closed loop system able to get resources from Earth via Gateway. Additionally, the moon will act as a closed-loop testbed for Mars habitat operations and will employ various elements of in situ resource utilization (ISRU). Perhaps the longest running BLSS collective study to date is that of the European MELiSSA (Micro Ecological Life Support System Alternative) project, a circular life support system, established to gain knowledge on regenerative systems aimed at the highest degree of autonomy to produce food, water and oxygen from mission wastes. This setup has evaluated the use of discrete microbial compartments and a higher plant compartment to carry out the necessary life support functions to support crew. Such compartments utilize thermophilic anaerobes to break down human and inedible plant wastes, photoheterotrophic bacteria that can further metabolize volatile fatty acids, nitrifying bacteria that can convert ammonium to plant and microalgae available nitrates, and photoautotrophic bacteria and higher plants which will convert carbon dioxide to oxygen, purify water, and provide food for human consumption. This being the most advanced BLSS model example to date -aside from the Yuegong-1, Chinese Lunar Palace- with the most defined compartment composition, and the assumption that other BLSSs will build upon such models, it becomes relevant to study the effects of the lunar environment upon the microbes that are integral to a BLSS. In ramping up to advanced stage BLSS systems, relatively simple experiments can be conducted on Artemis missions using continuous culturing and sampling of the model microbes, subsequently subjected to DNA sequencing for mutational analysis monitoring and chemical analysis to assess the sustained ability to carry out their hallmark biochemical processes efficiently. A suite of microbes should be assessed, prioritizing those with the duel capacity to be utilized in an BLSS and the ability to biochemically facilitate ISRU goals, such as the ability to transform the biogeochemistry of Moon or Martian regolith into materials that can support crop growth or extract elements of industrial significance such as aluminum or iron.

Biological Life Support Systems↗

Design of Plant Gas Exchange Experiments in a Variable Pressure Growth Chamber

Sustainable human presence in extreme environments such as lunar and martian bases will require bioregenerative components to human life support systems where plants are used for generation of oxygen, food, and water. Reduced atmospheric pressures will be used to minimize mass and engineering requirements. Few studies have assessed the metabolic and developmental responses of plants to reduced pressure and varied oxygen atmospheres. The first tests of hypobaric pressures on plant gas exchange and biomass production at the Johnson Space Center will be initiated in January 1996 in the Variable Pressure Growth Chamber (VPGC), a large, closed plant growth chamber rated for 10.2 psi. Experiments were designed and protocols detailed for two complete growouts each of lettuce and wheat to generate a general database for human life support requirements and to answer questions about plant growth processes in reduced pressure and varied oxygen environments. The central objective of crop growth studies in the VPGC is to determine the influence of reduced pressure and reduced oxygen on the rates of photosynthesis, dark respiration, evapotranspiration and biomass production of lettuce and wheat. Due to the constraint of one experimental unit, internal controls, called pressure transients, will be used to evaluate rates of CO2 uptake, O2 evolution, and H2O generation. Pressure transients will give interpretive power to the results of repeated growouts at both reduced and ambient pressures. Other experiments involve the generation of response functions to partial pressures of O2 and CO2 and to light intensity. Protocol for determining and calculating rates of gas exchange have been detailed. In order to build these databases and implement the necessary treatment combinations in short time periods, specific requirements for gas injections and removals have been defined. A set of system capability checks will include determination of leakage rates conducted prior to the actual crop growouts. Schedules of experimental events for lettuce and wheat are outlined and include replications in time of diurnal routines, pressure transients, variable pO2, pO2/pCO2 ratio, and light intensity responses.

Corey, Kenneth A.↗

Omics-Driven Biotechnology for Industrial Applications

Biomanufacturing is a key component of biotechnology that uses biological systems to produce bioproducts of commercial relevance, which are of great interest to the energy, material, pharmaceutical, food, and agriculture industries. Biotechnology-based approaches, such as synthetic biology and metabolic engineering are heavily reliant on “omics” driven systems biology to characterize and understand metabolic networks. Knowledge gained from systems biology experiments aid the development of synthetic biology tools and the advancement of metabolic engineering studies toward establishing robust industrial biomanufacturing platforms. In this review, we discuss recent advances in “omics” technologies, compare the pros and cons of the different “omics” technologies, and discuss the necessary requirements for carrying out multi-omics experiments. We highlight the influence of “omics” technologies on the production of biofuels and bioproducts by metabolic engineering. Finally, we discuss the application of “omics” technologies to agricultural and food biotechnology, and review the impact of “omics” on current COVID-19 research.

59 BASIC BIOLOGICAL SCIENCES↗

Development of Laboratory Testbeds Simulating Real-World Greenhouse Gas Emissions [Slides]

In this presentation, I discussed the development of laboratory test beds that simulate field greenhouse gas emissions. Field experiments are used to test new landscape management practices that could reduce greenhouse gas emissions. However, field experiments are very expensive and time consuming. Laboratory experiments are generally more cost efficient and timesaving. There are methods that can be used to make these laboratory tests more closely replicate natural systems for the improvement of agricultural lands used in biofuel and food production. The goal of this project is to develop a laboratory testbed that will mimic field greenhouse gas emissions. To achieve this goal, we used soil cores taken from agricultural and marginal lands, then attached fiberglass wicks to improve the drainage system in the core to simulate a more natural hydraulic system. The combination of using native soils and fiberglass wicks should demonstrate similar greenhouse gas emissions between the lab and the field. Our preliminary results suggest that the carbon dioxide and nitrous oxide flux from the agricultural land site were comparable to the greenhouse soil cores equipped with the drainage wicks. The data suggests that laboratory testbeds can be used to mimic field greenhouse gas emissions if parameters, such as soil water content are in a set range.

54 ENVIRONMENTAL SCIENCES↗

Pressure Control System Design for a Closed Crop Growth Chamber

The Controlled Ecological Life Support System (CELSS) is an area of active research at NASA. CELSS is a plant-based bioregenerative life support system for long term manned space flights where resupply is costly or impractical. The plants in a CELSS will function to convert the carbon dioxide (exhaled by the crew) into oxygen, purify non-potable water into potable quality water, and provide food for the crew. Prior to implementing a CELSS life support system, one must have knowledge on growing plants in a closed chamber under low gravity. This information will come from research to be conducted on the CELSS Test Facility that will operate on the Space Station Freedom. Currently a ground-based CELSS Test Facility is being built at NASA Ames Research Center. It is called the EDU (Engineering Development Unit). This system will allow researchers to identify issues that may cause difficulties in the development of the CELSS Test Facility and aid in the development of new needed technologies. The EDU consists of a 1 m2 crop growth chamber that is surrounded by a containment enclosure. The containment enclosure isolates the system so there is very little mass and thermal exchange with the ambient. The leakage rate is on the order of 1 % of the enclosure's volume per day (with 0.2S psi pressure difference). The thermal leakage is less than 0.5% of the electrical power supplied to the system per degree Celsius difference from the surrounding. The pressure in the containment enclosure is regulated at 62.5 Pa below the ambient by an active controller. The goal is to maintain this set point for a variety of conditions, such as a range of operating temperatures, heat load variations that occur when the lights are turned on and off, and fluctuations in ambient pressure. In addition certain transition tracking performance is required. This paper illustrates the application of some advanced systems control methods to the task of synthesizing the EDU's pressure control system.

Tsai, K.↗

Crew Exploration Vehicle Potable Water System Verification Description

A stored water system on the crew exploration vehicle (CEV) will supply the crew with potable water for: drinking and food rehydration, hygiene, medical needs, sublimation, and various contingency situations. The current baseline biocide for the stored water system is ionic silver, similar in composition to the biocide used to maintain the quality of the water, transferred from the orbiter to the International Space Station, stored in contingency water containers. In the CEV water system, a depletion of the ionic silver biocide is expected due to ionic silver-plating onto the surfaces of materials within the CEV water system, thus negating its effectiveness as a biocide. Because this may be the first time NASA is considering a stored water system for long-term missions that do not maintain a residual biocide, a team of experts in materials compatibility, biofilms and point-of-use filters, surface treatment and coatings, and biocides has been created to pinpoint concerns and perform the testing that will help alleviate concerns related to the CEV water system.

Tuan, George↗

Improved Thermal-Insulation Systems for Low Temperatures

Improved thermal-insulation materials and structures and the techniques for manufacturing them are undergoing development for use in low-temperature applications. Examples of low-temperature equipment for which these thermal insulation systems could provide improved energy efficiency include storage tanks for cryogens, superconducting electric-power-transmission equipment, containers for transport of food and other perishable commodities, and cold boxes for low-temperature industrial processes. These systems could also be used to insulate piping used to transfer cryogens and other fluids, such as liquefied natural gas, refrigerants, chilled water, crude oil, or low-pressure steam. The present thermal-insulation systems are layer composites based partly on the older class of thermal-insulation systems denoted generally as multilayer insulation (MLI). A typical MLI structure includes an evacuated jacket, within which many layers of radiation shields are stacked or wrapped close together. Low-thermal-conductivity spacers are typically placed between the reflection layers to keep them from touching. MLI can work very well when a high vacuum level (less than 10(exp-4) torr) is maintained and utmost care is taken during installation, but its thermal performance deteriorates sharply as the pressure in the evacuated space rises into the soft vacuum range [pressures greater than 0.1 torr (greater than 13 Pa)]. In addition, the thermal performance of MLI is extremely sensitive to mechanical compression and edge effects and can easily decrease from one to two orders of magnitude from its ideal value even when the MLI is kept under high vacuum condition. The present thermal-insulation systems are designed to perform well under soft vacuum level, in particular the range of 1 to 10 torr. They are also designed with larger interlayer spacings to reduce vulnerability to compression (and consequent heat leak) caused by installation and use. The superiority of these systems is the synergistic effect of improvements in materials, design, and manufacture.

Fesmire, James E.↗

How Well do Earth System Models Capture Apparent Relationships Between Phytoplankton Biomass and Environmental Variables?

Abstract As phytoplankton form the base of the marine food web, understanding the controls on their abundance is fundamental to understanding marine ecology and its sensitivity to global climate change. While many Earth System Models (ESMs) predict phytoplankton biomass, it is unclear whether they properly capture the mechanistic relationships that control this quantity in the real ocean. We used Random Forest analysis to analyze the output of 13 ESMs as well as two observational data sets. The target variable was phytoplankton carbon and the predictors included environmental parameters known to influence phytoplankton, including nutrients, light, mixed layer depth, salinity, temperature, and upwelling. We examined the following: (a) What fractions of variability in ESMs and observations can be linked to the large‐scale environmental variables simulated by ESMs? (b) What are the dominant predictors and relationships affecting phytoplankton biomass? (c) How well do ESMs simulate phytoplankton carbon and do they simulate the relationships we see in observations? About 88%–96% of the variability in observational data sets and greater than 98% in the ESMs was accounted for by environmental variables known to influence phytoplankton biomass. The dominant predictors in the observational data sets were shortwave radiation and dissolved iron, with temperature and ammonium also relatively important. All the ESMs show that shortwave radiation is the most important variable and most of them predict the right sign of sensitivity to most variables. However, the models predict that biomass reaches maximum levels at unrealistically low levels of iron and unrealistically high levels of light.

Environmental Sciences & Ecology↗

Mass balances for a biological life support system simulation model

Design decisions to aid the development of future space based biological life support systems (BLSS) can be made with simulation models. The biochemistry stoichiometry was developed for: (1) protein, carbohydrate, fat, fiber, and lignin production in the edible and inedible parts of plants; (2) food consumption and production of organic solids in urine, feces, and wash water by the humans; and (3) operation of the waste processor. Flux values for all components are derived for a steady state system with wheat as the sole food source. The large scale dynamics of a materially closed (BLSS) computer model is described in a companion paper. An extension of this methodology can explore multifood systems and more complex biochemical dynamics while maintaining whole system closure as a focus.

Volk, Tyler↗

Systemic Risk From the Perspective of Climate, Environmental and Disaster Risk Science and Practice

Understanding and managing systemic risk is more important than ever due to our immense global connectivity (e.g., between sectors, such as food-health-water-energy, countries and continents, down to individuals). Despite the fact that the notion of systemic risk is several decades old, the term is used in diverse ways across different disciplines (e.g., financial systems, medicine, earth system sciences, disaster risk research and climate science). Triggered by the repercussions of the global financial crisis of the late 2000s, and more recently the COVID-19 pandemic, which are clear realization of systemic risk, the perception of systemic risk has often been focused on global and catastrophic or even existential risks. Systemic risk, however, can be seen as a feature of systems at all possible scales (e.g., global, national, regional, local) with system boundaries varying depending on the context. Addressing current societal challenges, such as climate change, in terms of systemic risk requires integrating different systems perspectives and fostering system thinking, while implementing key intergovernmental agendas, such as the Paris Agreement, the Sendai Framework for Disaster Risk Reduction and the Sustainable Development Goals. Based on insights gained and knowledge collected from an expert workshop, literature review and expert elicitation, we give an integrated perspective of climate, environmental and disaster risk science and practice on systemic risk as summarized in a Briefing Note to the International Science Council. We provide an overview of concepts of systemic risk that have evolved over time and identify commonalities across terminologies and perspectives associated with systemic risk used in different contexts. Key attributes of systemic risk are outlined without prescribing a single definition, and information and data requirements are discussed that are essential for a better and more actionable understanding of the systemic nature of risk. Finally, the opportunities to connect research and policy for addressing systemic risk are highlighted.

systemic risk↗

Logistics of defense: The contribution of endomembranes to plant innate immunity

Phytopathogens cause plant diseases that threaten food security. Unlike mammals, plants lack an adaptive immune system and rely on their innate immune system to recognize and respond to pathogens. Plant response to a pathogen attack requires precise coordination of intracellular traffic and signaling. Spatial and/or temporal defects in coordinating signals and cargo can lead to detrimental effects on cell development. The role of intracellular traffic comes into a critical focus when the cell sustains biotic stress. In this review, we discuss the current understanding of the post-immune activation logistics of plant defense. Specifically, we focus on packaging and shipping of defense-related cargo, rerouting of intracellular traffic, the players enabling defense-related traffic, and pathogen-mediated subversion of these pathways. We highlight the roles of the cytoskeleton, cytoskeleton–organelle bridging proteins, and secretory vesicles in maintaining pathways of exocytic defense, acting as sentinels during pathogen attack, and the necessary elements for building the cell wall as a barrier to pathogens. We also identify points of convergence between mammalian and plant trafficking pathways during defense and highlight plant unique responses to illustrate evolutionary adaptations that plants have undergone to resist biotic stress.

59 BASIC BIOLOGICAL SCIENCES↗

AmeriFlux US-UR9 Farson - UCRB

This is the AmeriFlux version of the carbon flux data for the site US-UR9 Farson - UCRB. Site Description - This site is located in Farson, Wyoming, within a flat agricultural area irrigated by a center pivot system. The region experiences a cold semi-arid climate, characterized by hot summers and cold winters. The field is primarily cultivated with alfalfa, which thrives under the irrigation provided by the pivot system.

Neale, Christopher [Daugherty Water for Food Insti↗

Binding Free Energy Analysis of Colicin D, E3 and E8 to Their Respective Cognate Immunity Proteins Using Computational Simulations

Colicins are antimicrobial proteins produced by bacteria for the purpose of destroying neighboring bacteria. Colicin activity is neutralized by a specific cognate immunity protein in order to protect the host. This study investigates the structural and binding mechanisms underlying the interaction of colicin-D, -E3 and -E8 to their respective immunity proteins (ImD, Im3 and Im8) using structure prediction, molecular dynamics (MD) simulations and MM-PBSA approach of free energy calculations. High-confidence colicin-immunity (Col-Im) complex structures predicted using AlphaFold2 were subjected to MD simulations of 150 ns with GROMACS and were analyzed for the binding free energy calculation using gmx_MMPBSA. Results showed that the complex of Col_E3-Im3 exhibited the most favorable binding free energy, driven by strong van der Waals and electrostatic interactions. Col_D-ImD and Col_E8-Im8 also showed the favorable binding. Electrostatics and hydrogen bonding emerged as a key factor driving binding and stability, while polar solvation acted as a destabilizing factor across all systems. These outcomes provide an understanding of the molecular mechanisms of Col-Im systems, with potential applications for developing natural antimicrobials for food safety.

Biochemistry & Molecular Biology↗

Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America

Some insects form symbioses in which actinomycetes provide defense against pathogens by making antimicrobials. The range of chemical strategies employed across these associations, and how these strategies relate to insect lifestyle, remains underexplored. We assessed subsocial passalid beetles of the species Odontotaenius disjunctus , and their frass (fecal material), which is an important food resource within their galleries, as a model insect/actinomycete system. Through chemical and phylogenetic analyses, we found that O. disjunctus frass collected across eastern North America harbored multiple lineages of Streptomyces and diverse antimicrobials. Metabolites detected in frass displayed synergistic and antagonistic inhibition of a fungal entomopathogen, Metarhizium anisopliae , and multiple streptomycete isolates inhibited this pathogen when co-cultivated directly in frass. These findings support a model in which the lifestyle of O. disjunctus accommodates multiple Streptomyces lineages in their frass, resulting in a rich repertoire of antimicrobials that likely insulates their galleries against pathogenic invasion.

59 BASIC BIOLOGICAL SCIENCES↗

Space vehicle with artificial gravity and earth-like environment

A space vehicle adapted to provide an artificial gravity and earthlike atmospheric environment for occupants is disclosed. The vehicle comprises a cylindrically shaped, hollow pressure-tight body, one end of which is tapered from the largest diameter of the body, the other end is flat and transparent to sunlight. The vehicle is provided with thrust means which rotates the body about its longitudinal axis, generating an artificial gravity effect upon the interior walls of the body due to centrifugal forces. The walls of the tapered end of the body are maintained at a temperature below the dew point of water vapor in the body and lower than the temperature near the transparent end of the body. The controlled environment and sunlight permits an earth like environment to be maintained wherein the CO2/O2 is balanced, and food for the travelers is supplied through a natural system of plant life grown on spacecraft walls where soil is located.

Gray, V. H.↗