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SeaWiFS Technical Report Series: SeaWiFS Calibration and Validation Quality Control Procedures - Volume 38

This document provides five brief reports that address several quality control procedures under the auspices of the Calibration and Validation Element (CVE) within the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) Project. Chapter 1 describes analyses of the 32 sensor engineering telemetry streams. Anomalies in any of the values may impact sensor performance in direct or indirect ways. The analyses are primarily examinations of parameter time series combined with statistical methods such as auto- and cross-correlation functions. Chapter 2 describes how the various onboard (solar and lunar) and vicarious (in situ) calibration data will be analyzed to quantify sensor degradation, if present. The analyses also include methods for detecting the influence of charged particles on sensor performance such as might be expected in the South Atlantic Anomaly (SAA). Chapter 3 discusses the quality control of the ancillary environmental data that are routinely received from other agencies or projects which are used in the atmospheric correction algorithm (total ozone, surface wind velocity, and surface pressure; surface relative humidity is also obtained, but is not used in the initial operational algorithm). Chapter 4 explains the procedures for screening level-, level-2, and level-3 products. These quality control operations incorporate both automated and interactive procedures which check for file format errors (all levels), navigation offsets (level-1), mask and flag performance (level-2), and product anomalies (all levels). Finally, Chapter 5 discusses the match-up data set development for comparing SeaWiFS level-2 derived products with in situ observations, as well as the subsequent outlier analyses that will be used for evaluating error sources.

Hooker, Stanford B.↗

Assessing the Completeness of Occupational Exposure Data in the Lifetime Surveillance of Astronaut Health

INTRODUCTION: Longitudinal analysis on how spaceflight affects human health requires significant amounts of data. Missing data, especially if missing in a non-random fashion, could be a significant challenge to the success and validity of ongoing occupational surveillance and research. Astronaut occupational health data have been collected since 1959 in various formats and as part of several flight programs. As a result of changing methodologies over this span, epidemiologists in the NASA Lifetime Surveillance of Astronaut Health (LSAH) project regularly compile data sets with important exposure or outcome data missing. METHODS: NASA medical records of astronauts participating in voluntary annual LSAH examinations were reviewed and compiled to develop Individual Exposure Profiles (IEP) for each astronaut. These data were supplemented by an interview. If the interview yielded medically relevant information absent from the medical record, that information was considered an update. The IEPs were analyzed to identify trends regarding the characteristics of astronauts who provided updates and what kinds of information were consistently being updated. RESULTS: To date, 190 astronauts have participated in the IEP project. Medical information was updated for 119 individuals during these interviews. The astronauts' likelihood of updating their record upon interview was not significantly related to their spaceflight experience, era of active spaceflight, or duration of longest spaceflight. The most commonly updated categories of medical information were issues encountered during spaceflights, including CO2 symptoms, vision changes, back pain, headaches, and space motion sickness. DISCUSSION: The most commonly updated categories correspond to areas where LSAH has ongoing analysis efforts and therefore do not appear to have been reported at random. This presentation will address identification of missing astronaut health data and trends, forward work identified by the IEP project and how this information may be used for future LSAH data gap analyses.

Sieker, Jeremy↗

Integrated Display System for Low Visibility Landing and Surface Operations

This report summarizes the software products and system architectures developed by Lockheed Martin in support of the Low Visibility Landing and Surface Operations (LVLASO) program at NASA Langley Research Center. It presents an overview of the technical aspects, capabilities, and system integration issues associated with an integrated display system (IDS) that collects, processes and presents information to an aircraft flight crew during all phases of landing, roll-out, turn-off, inbound taxi, outbound taxi and takeoff. Communications hardware, drivers, and software provide continuous real-time data at varying rates and from many different sources to the display programs for presentation on a head-down display (HDD) and/or a head-up display (HUD). An electronic moving map of the airport surface is implemented on the HDD which includes the taxi route assigned by air traffic control, a text messaging system, and surface traffic and runway status information. Typical HUD symbology for navigation and control of the aircraft is augmented to provide aircraft deceleration guidance after touchdown to a pilot selected exit and taxi guidance along the route assigned by ATC. HUD displays include scene-linked symbolic runways, runway exits and taxiways that are conformal with the actual locations on the airport surface. Display formats, system architectures, and the various IDS programs are discussed.

Beskenis, Sharon Otero↗

Angle-of-Attack Sweep with Mesh Adaptation for High-Lift Configurations

This paper reports an angle-of-attack (alpha) sweep study with mesh adaptation for the high-lift configuration - JAXA Standard Model (JSM) from the 3rd AIAA High-Lift Prediction Workshop. The method of angle-of-attack (alpha) continuation, where the flow-field at a certain angle of attack is initialized from the solution obtained at the previous angle of attack, is applied to adapted unstructured meshes. Since the meshes are continuously adapted, flow-field initialization on a particular mesh for a certain angle is done through linear interpolation of the solution from the previous mesh. Interpolated initial conditions usually prevent massively separated solutions that may otherwise occur with impulsive initial conditions. Alpha continuations are done in both forward (increasing alpha) and reverse (decreasing alpha) directions, resulting in the formation of hysteresis loops. Skin frictions are compared with the wind-tunnel oil flow images and pressure coefficients are compared with the wind-tunnel values, at selected angles of attack. Final paper will include results also for the high-lift version of the Common Research Model (CRM-HL) from the 4th AIAA High-Lift Prediction Workshop. A thorough comparison of the results will also be made with the available wind-tunnel data.

Mesh Adaptation↗

Description, Usage, and Validation of the MVL-15 Modified Vortex Lattice Analysis Capability

MVL-15 is the most recent version of the Modified Vortex-Lattice (MVL) code developed within the Aerodynamics Systems Analysis Branch (ASAB) at NASA LaRC. The term "modified" refers to the primary modification of the core vortex-lattice methodology: inclusion of viscous aerodynamics tables that are linked to the linear solution via iterative processes. The inclusion of the viscous aerodynamics inherently converts the MVL-15 from a purely analytic linearized method to a semi-empirical blend which retains the rapid execution speed of the linearized method while empirically characterizing the section aerodynamics at all spanwise lattice points. The modification provides a means to assess non-linear effects on lift that occur at angles of attack near stall, and provides a means to determine the drag associated with the application of design strategies for lift augmentation such as the use of flaps or blowing. The MVL-15 code is applicable to the analyses of aircraft aerodynamics during cruise, but it is most advantageously applied to the analysis of aircraft operating in various high-lift configurations. The MVL methodology has been previously conceived and implemented; the initial concept version was delivered to the ASAB in 2001 (van Dam, C.), subsequently revised (Gelhausen, P. and Ozoroski, T. 2002 / AVID Inc., Gelhausen, P., and Roberts, M. 2004), and then overhauled (Ozoroski, T., Hahn, A. 2008). The latest version, MVL-15 has been refined to provide analysis transparency and enhanced to meet the analysis requirements of the Environmentally Responsible Aviation (ERA) Project. Each revision has been implemented with reasonable success. Separate applications of the methodology are in use, including a similar in-house capability, developed by Olson, E. that is tailored for structural and acoustics analyses. A central premise of the methodology is that viscous aerodynamic data can be associated with analytic inviscid aerodynamic results at each spanwise wing section, thereby providing a pathway to map viscous data to the inviscid results. However, a number of factors can sidetrack the analysis consistency during various stages of this process. For example, it should be expected that the final airplane lift curve and drag polar results depend strongly on the geometry and aerodynamics of the airfoil section; however, flap deflections and flap chord extensions change the local reference geometry of the input airfoil, the airplane wing, the tabulated non-dimensional viscous aerodynamics, and the spanwise links between the linear and the viscous aerodynamics. These changes also affect the bound circulation and therefore, calculation and integration of the induced angle of attack and induced drag. MVL-15 is configured to ensure these types of challenges are properly addressed. This report is a comprehensive manual describing the theory, use, and validation of the MVL-15 analysis tool. Section 3 summarizes theoretical, procedural, and characteristic features of MVL-15, and includes a list of the files required to setup, execute, and summarize an analysis. Section 4, Section 5, Section 6, and Section 7 combine to comprise the User's Guide portions of this report. The MVL-15 input and output files are described in Section 4 and Section 5, respectively; the descriptions are supplemented with example files and information about the file formats, parameter definitions, and typical parameter values. Section 6 describes the Wing Geometry Setup Utility and the 2d-Variants Utility files that simplify and assist setting up a consistent set of MVL-15 geometry and aerodynamics input parameters and input files. Section 7 describes the use of the 3d-Results Presentation Utility file that can be used to automatically create summary tables and charts from the MVL-15 output files. Section 8 documents the Validation Results of an extensive and varied validation test matrix, including results of an airplane analysis representative of the ERA Program. A start-to-finish example of the airplane analysis procedure is described in Section 7.

Ozoroski, Thomas A.↗

SeaWiFS technical report series. Volume 21: The heritage of SeaWiFS. A retrospective on the CZCS NIMBUS Experiment Team (NET) program

The Sea-viewing Wide Field-of-view Sensor (SeaWiFS) mission is based on the scientific heritage of the Coastal Zone Color Scanner (CZCS), a proof-of-concept instrument carried on the National Aeronautics and Space Administration (NASA) NIMBUS-7 environmental satellite for the purpose of measuring upwelling radiance from the ocean surface. The CZCS mission provided the first observations of ocean color from space, and over the mission lifetime of 1978-1986, allowed oceanographers an initial opportunity to observe the variable patterns of global biological productivity. One of the key elements of the CZCS mission was the formation of the CZCS NIMBUS Experiment Team (NET), a group of optical physicists and biological oceanographers. The CZCS NET was designated to validate the accuracy of the CZCS radiometric measurements and to connect the instrument's measurements to standard measures of oceanic biological productivity and optical seawater clarity. In the period following the cessation of CZCS observations, some of the insight and experience gained by the CZCS NET activity has dissipated as several proposed follow-on sensors failed to achieve active status. The Sea WiFS mission will be the first dedicated orbital successor to CZCS it in turn precedes observations by the Moderate Resolution Imaging Spectroradiometer (MODIS) of the Earth Observing System (EOS). Since the CZCS NET experience is an important model for Sea WiFS and MODIS surface truth efforts, this document is intended to provide a comprehensive review of the validation of oceanographic data for the first orbital ocean color sensor mission. This document also summarizes the history of the CZCS NET activities. The references listed in the Bibliography are a listing of published scientific research which relied upon the CZCS BET algorithms, or research which was conducted on the basis of CZCS mission elements.

Acker, James G.↗

Research Supporting Satellite Communications Technology

This report describes the second year of research effort under the grant Research Supporting Satellite Communications Technology. The research program consists of two major projects: Fault Tolerant Link Establishment and the design of an Auto-Configurable Receiver. The Fault Tolerant Link Establishment protocol is being developed to assist the designers of satellite clusters to manage the inter-satellite communications. During this second year, the basic protocol design was validated with an extensive testing program. After this testing was completed, a channel error model was added to the protocol to permit the effects of channel errors to be measured. This error generation was used to test the effects of channel errors on Heartbeat and Token message passing. The C-language source code for the protocol modules was delivered to Goddard Space Flight Center for integration with the GSFC testbed. The need for a receiver autoconfiguration capability arises when a satellite-to-ground transmission is interrupted due to an unexpected event, the satellite transponder may reset to an unknown state and begin transmitting in a new mode. During Year 2, we completed testing of these algorithms when noise-induced bit errors were introduced. We also developed and tested an algorithm for estimating the data rate, assuming an NRZ-formatted signal corrupted with additive white Gaussian noise, and we took initial steps in integrating both algorithms into the SDR test bed at GSFC.

Horan Stephen↗

Smectite Formation in the Presence of Sulfuric Acid: Implications for Acidic Smectite Formation on Early Mars

The excess of orbital detection of smectite deposits compared to carbonate deposits on the martian surface presents an enigma because smectite and carbonate formations are both favored alteration products of basalt under neutral to alkaline conditions. We propose that Mars experienced acidic events caused by sulfuric acid (H2SO4) that permitted phyllosilicate, but inhibited carbonate, formation. To experimentally verify this hypothesis, we report the first synthesis of smectite from Mars-analogue glass-rich basalt simulant (66 wt% glass, 32 wt% olivine, 2 wt% chromite) in the presence of H2SO4 under hydrothermal conditions (approximately 200 degC). Smectites were analyzed by X-ray diffraction, Mossbauer spectroscopy, visible and near-infrared reflectance spectroscopy and electron microprobe to characterize mineralogy and chemical composition. Solution chemistry was determined by Inductively Coupled Plasma Mass Spectrometry. Basalt simulant suspensions in 11-42 mM H2SO4 were acidic with pH less than or equal to 2 at the beginning of incubation and varied from acidic (pH 1.8) to mildly alkaline (pH 8.4) at the end of incubation. Alteration of glass phase during reaction of the basalt simulant with H2SO4 led to formation of the dioctahedral smectite at final pH approximately 3 and trioctahedral smectite saponite at final pH approximately 4 and higher. Anhydrite and hematite formed in the final pH range from 1.8 to 8.4 while natroalunite was detected at pH 1.8. Hematite was precipitated as a result of oxidative dissolution of olivine present in Adirondack basalt simulant. Formation of secondary phases, including smectite, resulted in release of variable amounts of Si, Mg, Na and Ca while solubilization of Al and Fe was low. Comparison of mineralogical and solution chemistry data indicated that the type of smectite (i.e., dioctahedral vs trioctahedral) was likely controlled by Mg leaching from altering basalt and substantial Mg loss created favorable conditions for formation of dioctahedral smectite. We present a model for global-scale smectite formation on Mars via acid-sulfate conditions created by the volcanic outgassing of SO2 in the Noachian and early Hesperian.

Smectite↗

Link Between Arctic Tropospheric BrO Explosion Observed from Space and Sea-Salt Aerosols from Blowing Snow Investigated Using Ozone Monitoring Instrument BrO Data and GEOS-5 Data Assimilation System

Bromine radicals (Br + BrO) are important atmospheric species owing to their ability to catalytically destroy ozone as well as their potential impacts on the oxidative pathways of many trace gases, including dimethylsulfide and mercury. Using space-based observations of BrO, recent studies have reported rapid enhancements of tropospheric BrO over large areas (so called "BrO explosions") connected to near-surface ozone depletion occurring in polar spring. However, the source(s) of reactive bromine and mechanism(s) that initiate these BrO explosions are uncertain. In this study, we investigate the relationships between Arctic BrO explosions and two of the proposed sources of reactive bromine: sea-salt aerosol (SSA) generated from blowing snow and first-year (seasonal) sea ice. We use tropospheric column BrO derived from the Ozone Monitoring Instrument (OMI) in conjunction with the Goddard Earth Observing System Version 5 (GEOS-5) data assimilation system provided by National Aeronautics and Space Administration Global Modeling and Assimilation Office. Case studies demonstrate a strong association between the temporal and spatial extent of OMI-observed BrO explosions and the GEOS-5 simulated blowing snow-generated SSA during Arctic spring. Furthermore, the frequency of BrO explosion events observed over the 11-year record of OMI exhibits significant correlation with a time series of the simulated SSA emission flux in the Arctic and little to no correlation with a time series of satellite-based first-year sea ice area. Therefore, we conclude that SSA generated by blowing snow is an important factor in the formation of the BrO explosion observed from space during Arctic spring.

blowing snow↗

The Isothermal Dendritic Growth Experiment (IDGE): USMP-4 One-Year-Report

Dendrites describe the tree-like crystal morphology commonly assumed in many material systems--particularly in metals and alloys that freeze from supercooled or supersaturated melts. There remains a high level of engineering interest in dendritic solidification because of the role of dendrites in the determination of cast alloy microstructures. Microstructure plays a key role in determining the physical properties of cast or welded products. In addition, dendritic solidification provides an example of non-equilibrium physics and is one of the simplest non-trivial examples of dynamic pattern formation, where an amorphous melt, under simple starting conditions, evolves into a complex ramified microstructure. Although it is well-known that dendritic growth is controlled by the transport of latent heat from the moving solid-melt interface as the dendrite advances into a supercooled melt, an accurate, and predictive model has not been developed. Current theories consider: 1) the transfer of heat or solute from the solid-liquid interface into the melt, and 2) the interfacial crystal growth and growth selection physics for the interface. However, the effects of gravity-induced convection on the transfer of heat from the interface prevent either element from being adequately tested solely under terrestrial conditions. The Isothermal Dendritic Growth Experiment (IDGE) constituted a series of three NASA-supported microgravity experiments, all of which flew aboard the space shuttle, Columbia. This experimental space flight series was designed and operated to grow and record dendrite solidification in the absence of gravity-induced convective heat transfer, and thereby produce a wealth of benchmark-quality data for testing solidification scaling laws. The data collection from the on-orbit phase of the IDGE flight series is now complete. We are currently completing analyses and moving towards final data archiving.

Glicksman, M. E.↗

New Detections of Radio Minihalos in Cool Cores of Galaxy Clusters

Cool cores of some galaxy clusters exhibit faint radio minihalos. Their origin is unclear, and their study has been limited by their small number. We undertook a systematic search for minihalos in a large sample of X-ray luminous clusters with high-quality radio data. In this article, we report four new minihalos (A 478, ZwCl 3146,RXJ 1532.9+3021, and A 2204) and five candidates found in the reanalyzed archival Very Large Array observations.The radio luminosities of our minihalos and candidates are in the range of 102325 W Hz1 at 1.4 GHz, which is consistent with these types of radio sources. Their sizes (40160 kpc in radius) are somewhat smaller than those of previously known minihalos. We combine our new detections with previously known minihalos, obtaining a total sample of 21 objects, and briefly compare the cluster radio properties to the average X-ray temperature and the total masses estimated from Planck.We find that nearly all clusters hosting minihalos are hot and massive. Beyond that, there is no clear correlation between the minihalo radio power and cluster temperature or mass (in contrast with the giant radio halos found in cluster mergers, whose radio luminosity correlates with the cluster mass). Chandra X-ray images indicate gas sloshing in the cool cores of most of our clusters, with minihalos contained within the sloshing regions in many of them. This supports the hypothesis that radio-emitting electrons are reaccelerated by sloshing. Advection of relativistic electrons by the sloshing gas may also play a role in the formation of the less extended minihalos.

X-ray Polarization↗

MSL SAM-Like Evolved Gas Analyses of Si-rich Amorphous Materials

Chemical and mineralogical analyses of several samples from Murray Formation mudstones and Stimson Formation sandstones by the Mars Science Laboratory (MSL) revealed the presence of Si-rich amorphous or poorly ordered materials. It is possible to identify the presence of high-SiO2 vs. lower SiO2 amorphous materials (e.g., basaltic glasses), based on the position of the resulting wide diffraction features in XRD patterns from the Chemistry and Mineralogy (CheMin) instrument, but it is not possible to distinguish between several candidate high-SiO2 amorphous materials such as opal-A or rhyolitic glass. In the Buckskin (BS) sample from the upper Murray Formation, and the Big Sky (BY) and Greenhorn (GH) samples from the Stimson Formation, analyses by the Sample Analysis at Mars (SAM) instrument showed very broad H2O evolutions during sample heating at temperatures >450-500degC which had not been observed from previous samples. BS also had a significant broad evolution <450-500degC. We have undertaken a laboratory study targeted at understanding if the data from SAM can be used to place constraints on the nature of the amorphous phases. SAM-like evolved gas analyses have been performed on several opal and rhyolitic glass samples. Opal-A samples exhibited wide <500degC H2O evolutions, with lesser H2O evolved above ~500degC. H2O evolution traces from rhyolitic glasses varied, having either two broad H2O peaks, <300degC and >500degC, or a broad peak centered around 400degC. For samples that produced two evolutions, the lower temperature peak is more intense than the higher temperature peak, a trend also exhibited by opal-A. This trend is consistent with data from BS, but does not seem consistent with data from BY and GH which evolved most of their H2O >500degC. It may be that dehydration of opal-A and/or rhyolitic glass can result in some preferential loss of lower temperature H2O, to produce traces that more closely resemble BY and GH. This is currently under investigation and results will be reported.

McAdam, Amy↗

Review of the Natural History of Renal Stones in U.S. Astronauts

BACKGROUND: The formation of renal stones in astronauts may pose a considerable threat to crew health and to the completion of mission objectives. Although a history of prior renal stones is an exclusion criterion for initial selection, there have been reported cases of renal stones that have developed after selection. This led to the establishment, nine years ago, of a standardized protocol to screen for the development of renal stones pre-and post-spaceflight in all active astronauts. OVERVIEW: Literature regarding stone makeup, size, location, time to passage, and outcomes were reviewed, as well as incidence of renal stones in astronaut analog populations such as military pilots and the general population. The Lifetime Surveillance of Astronaut Health (LSAH) database was queried and as of 2015 there have been 22 crew members who have reported 36 renal stone events from a population of 357 astronauts, with an incidence of 0.32 events per year. This compares favorably to the incidence of 0.37 to 0.46events per year in commercial pilots. DISCUSSION: The physiologic changes associated with spaceflight have been thought to present an increased risk for the development of stones, including hypercalciuria, decreased urine pH, and reduced urine volume. NASA’s screening program, based on a rigorous ultrasound method, has now provided nine years of clinical data used to reduce the risk of flying an astronaut with a renal stone that could threaten health and mission objectives. This work and data from the ultrasound screening program was used to update the clinical practice guideline for renal stone surveillance and treatment, and other NASA documents and programs that track this risk.

C McNerlin↗

Endogenous Lunar Volatiles: Insights into the Abundances of Volatiles in the Moon from Lunar Apatite

At the time of publication of New Views of the Moon, it was thought that the Moon was bone dry with less than about 1 ppb H2O. However in 2007, initial reports at the 38th Lunar and Planetary Science Conference speculated that H-species were present in both apatites and pyroclastic volcanic lunar glasses. These early reports were later confirmed through peer-review, which motivated many subsequent studies on magmatic volatiles in and on the Moon within the last decade. Some of these studies have cast into question the post-Apollo view of lunar formation, the distribution and sources of volatiles in the Earth-Moon system, and the thermal and magmatic evolution of the Moon. The mineral apatite has been one of the pillars of this new field of study, and it will be the primary focus of this abstract. Although apatite has been used both to understand the abundances of volatiles in lunar systems as well as the isotopic compositions of those volatiles, the focus here will be on the abundances of F, Cl, and H2O. This work demonstrates the utility of apatite in advancing our understanding of lunar volatiles, hence apatite should be among the topics covered in the endogenous lunar volatile chapter in NVM II. Truncated ternary plot of apatite X-site occupancy (mol%) from highlands apatite and mare basalt apatite plotted on the relative volatile abundance diagram from. The solid black lines delineate fields of relative abundances of F, Cl, and H2O (on a weight basis) in the melt from which the apatite crystallized. The diagram was constructed using available apatite/melt partitioning data for fluorine, chlorine, and hydroxyl.

McCubbin, Francis↗

IRAS 16293-2422: Evidence for Infall onto a Counter-Rotating Protostellar Accretion Disk

We report high spatial resolution VLA observations of the low-mass star-forming region IRAS 16293-2422 using four molecular probes: ethyl cyanide (CH3CH2CN)) methyl formate (CH3OCHO), formic acid (HCOOH), and the ground vibrational state of silicon monoxide (SiO). Ethyl cyanide emission has a spatial scale of approx. 20" and encompasses binary cores A and B as determined by continuum emission peaks. Surrounded by formic acid emission, methyl formate emission has a spatial scale of approx. 6" and is confined to core B. SiO emission shows two velocity components with spatial scales less than 2" that map approx. 2" northeast of the A and B symmetry axis. The redshifted SiO is approx. 2" northwest of blueshifted SiO along a position angle of approx. 135deg which is approximately parallel to the A and B symmetry axis. We interpret the spatial position offset in red and blueshifted SiO emission as due to rotation of a protostellar accretion disk and we derive approx. 1.4 Solar Mass, interior to the SiO emission. In the same vicinity, Mundy et al. (1986) also concluded rotation of a nearly edge-on disk from OVRO observations of much stronger and ubiquitous CO-13 emission but the direction of rotation is opposite to the SiO emission findings. Taken together, SiO and CO-13 data suggest evidence for a counter-rotating disk. Moreover, archival BIMA array CO-12C data show an inverse P Cygni profile with the strongest absorption in close proximity to the SiO emission, indicating unambiguous material infall toward the counter-rotating protostellar disk at a new source location within the IRAS 16293-2422 complex. The details of these observations and our interpretations are discussed.

Remijan, Anthony J.↗

DNA Damage Response to Low and High-LET in a Large Cohort of Mice and Humans and Latest Advancement in NASA Space Omics

This presentation will first focus on a thorough evaluation of the DNA damage response to both low and high-LET in a cohort of 76 mice primary skin fibroblast derived from 15 different strains or in human blood mononuclear cells derived from 550 healthy donors. In both the human and mice work, we have hypothesized that DNA repair capacity can be used as a marker to evaluate and differentiate individual radiation sensitivity. More specifically, this work is based on the concept that the combined time-dose dependence of radiation-induced foci (RIF) of p53-binding protein 1 (53BP1) following low-LET exposure contains sufficient information to infer sensitivity to any other LET. This work is one of the most extensive studies on the kinetics and possible genetic underpinnings of radiation-induced DNA damage and repair. Results on humans are still preliminary as we are still in the process of collecting and isolating primary blood mononuclear cells from 500 to 800 healthy subjects of European descent, 18-75 years of age, 50/50 male/female distribution. We have analyzed 53BP1+ RIF formation as well as oxidative stress and cell death in primary cells from 192 subjects in response to the same HZE particles as used in mice: 600 MeV/n Fe, 350 MeV/n Ar and 350 MeV/n Si, 1.1 and 3 particles/100m2, 4 and 24 hours after irradiation. The second part of the talk will focus on describing GeneLab: The NASA Systems Biology Platform for Space Omics Repository, Analysis and Visualization. NASA GeneLab is an open-access repository for omics datasets generated by biological experiments conducted in space or experiments relevant to spaceflight (e.g. simulated cosmic radiation, simulated microgravity, bed rest studies). Started as a repository designed to archive precious omics from space experiments, GeneLab has expanded its scope to maximize the intelligibility of the raw data (e.g. RNAseq, microarray, WGBS, metagenome), particularly for users with limited bioinformatics knowledge. As such GeneLab is now providing processed data derived from the raw data covering a large spectrum of omics (genome, epigenome, transcriptome, epitranscriptome, proteome, metabolome), to help users explore important questions: Which genes or proteins are expressed differently in space for various living organisms? What are the consequences arising from these changes? What specifics DNA mutations or epigenetic changes happen in space? What species or genetic features lead to better adaption to such a unique environment? In this presentation, we will report on the current and future objectives for GeneLab, and review recent published studies relating molecular changes observed in various animal models and tissue with microgravity, radiation, circadian rhythm, hydration and carbon dioxide conditions.

DNA repair kinetics↗

Geologic Mapping of Vesta

We report on a preliminary global geologic map of Vesta, based on data from the Dawn spacecraft's High- Altitude Mapping Orbit (HAMO) and informed by Low-Altitude Mapping Orbit (LAMO) data. This map is part of an iterative mapping effort; the geologic map has been refined with each improvement in resolution. Vesta has a heavily-cratered surface, with large craters evident in numerous locations. The south pole is dominated by an impact structure identified before Dawn's arrival. Two large impact structures have been resolved: the younger, larger Rheasilvia structure, and the older, more degraded Veneneia structure. The surface is also characterized by a system of deep, globe-girdling equatorial troughs and ridges, as well as an older system of troughs and ridges to the north. Troughs and ridges are also evident cutting across, and spiraling arcuately from, the Rheasilvia central mound. However, no volcanic features have been unequivocally identified. Vesta can be divided very broadly into three terrains: heavily-cratered terrain; ridge-and-trough terrain (equatorial and northern); and terrain associated with the Rheasilvia crater. Localized features include bright and dark material and ejecta (some defined specifically by color); lobate deposits; and mass-wasting materials. No obvious volcanic features are evident. Stratigraphy of Vesta's geologic units suggests a history in which formation of a primary crust was followed by the formation of impact craters, including Veneneia and the associated Saturnalia Fossae unit. Formation of Rheasilvia followed, along with associated structural deformation that shaped the Divalia Fossae ridge-and-trough unit at the equator. Subsequent impacts and mass wasting events subdued impact craters, rims and portions of ridge-and-trough sets, and formed slumps and landslides, especially within crater floors and along crater rims and scarps. Subsequent to the formation of Rheasilvia, discontinuous low-albedo deposits formed or were emplaced; these lie stratigraphically above the equatorial ridges that likely were formed by Rheasilvia. The last features to be formed were craters with bright rays and other surface mantling deposits. Executed progressively throughout data acquisition, the iterative mapping process provided the team with geologic proto-units in a timely manner. However, interpretation of the resulting map was hampered by the necessity to provide the team with a standard nomenclature and symbology early in the process. With regard to mapping and interpreting units, the mapping process was hindered by the lack of calibrated mineralogic information. Topography and shadow played an important role in discriminating features and terrains, especially in the early stages of data acquisition.

Vesta↗

Oxygen Isotopic Imaging of Refractory Inclusions from the Miller Range (MIL) 090019 CO3 Chondrite: A Perovskite Perspective

Calcium-Aluminum-rich Inclusions (CAIs) in primitive meteorites are the first solids to condense in the Solar System. The oxygen isotopic compositions recorded in various mineral components of CAIs provide clues about their origins and post-formation histories, recording processes such as condensation, melting, nebular alteration, and fluidrock reactions on the parent body. MIL 090019 is similar to some rare carbonaceous chondrites such as Acfer 094, DOM 08004/6 and ALH 77303 that contain high abundances of a variety of refractory inclusions. This provides an opportunity to study the oxygen isotopic record of different types of refractory inclusions within the same meteorite. We analyzed CAIs specifically targeting primary minerals that are direct nebular condensates, such as corundum and perovskite, with the goal of gaining insights into the O isotopic composition of the nebular gas(es) from which these CAIs condensed. As MIL 090019 is a classified as CO3.1, it shows some signs of thermal metamorphism, compared to the more primitive CO3 meteorites (e.g., DOM 08004/06). A second goal of this study is to search for evidence of nebular processes in phases such as perovskite and melilite that are susceptible to parent body alteration to varying degrees. We analyzed the oxygen isotopic compositions of various CAIs from the MIL 090019 CO3 carbonaceous chondrite by ion imaging using the NanoSIMS 50L (Nano Secondary Ion Mass Spectrometer) at JSC following methods described in. An advantage of ion imaging over traditional spot analyses is that it provides spatial context to the oxygen isotopic data. This work builds on previously reported oxygen isotopic composition of two other CAIs (CAI-44 and CAI-E2) from the same meteorite thin section.

Mane, P.↗