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At least 793 records · Page 44

Functional and Evolutionary Integration of a Fungal Gene With a Bacterial Operon

Abstract Siderophores are crucial for iron-scavenging in microorganisms. While many yeasts can uptake siderophores produced by other organisms, they are typically unable to synthesize siderophores themselves. In contrast, Wickerhamiella/Starmerella (W/S) clade yeasts gained the capacity to make the siderophore enterobactin following the remarkable horizontal acquisition of a bacterial operon enabling enterobactin synthesis. Yet, how these yeasts absorb the iron bound by enterobactin remains unresolved. Here, we demonstrate that Enb1 is the key enterobactin importer in the W/S-clade species Starmerella bombicola. Through phylogenomic analyses, we show that ENB1 is present in all W/S clade yeast species that retained the enterobactin biosynthetic genes. Conversely, it is absent in species that lost the ent genes, except for Starmerella stellata, making this species the only cheater in the W/S clade that can utilize enterobactin without producing it. Through phylogenetic analyses, we infer that ENB1 is a fungal gene that likely existed in the W/S clade prior to the acquisition of the ent genes and subsequently experienced multiple gene losses and duplications. Through phylogenetic topology tests, we show that ENB1 likely underwent horizontal gene transfer from an ancient W/S clade yeast to the order Saccharomycetales, which includes the model yeast Saccharomyces cerevisiae, followed by extensive secondary losses. Taken together, these results suggest that the fungal ENB1 and bacterial ent genes were cooperatively integrated into a functional unit within the W/S clade that enabled adaptation to iron-limited environments. This integrated fungal-bacterial circuit and its dynamic evolution determine the extant distribution of yeast enterobactin producers and cheaters.

59 BASIC BIOLOGICAL SCIENCES↗

Experimental data and theoretical analysis of an operating 100 kW wind turbine

Part of the cooperative effort between NASA and ERDA has been the design and the erection of an experimental wind turbine by the NASA-Lewis Research Center. This 100 kW turbine, designated the Mod-O, is located at the NASA Plum Brook site near Sandusky, Ohio. Experimental test data have been correlated with analyses of turbine loads and complete system behavior of the 100 kW Mod-O wind turbine generator over a broad range of steady state conditions, as well as during transient conditions. The deficit in the ambient wind field due to the upwind tower turbine support structure was found to be very significant in exciting higher harmonic loads associated with the flapping response of the blade in bending.

Linscott, B. S.↗

X-traktor: A Rookie Robot, Simple, Yet Complex, Impeccably Designed, a Very Innovative Multidisciplinary Engineering Masterpiece

FIRST is the acronym of For Inspiration and Recognition of Science and Technology. FIRST is a 501.C.3 non-profit organization whose mission is to generate an interest in science and engineering among today's young adults and youth. This mission is accomplished through a robot competition held annually in the spring of each year. NASAs Marshall Space Flight Center, Education Programs Department, awarded a grant to Lee High School, the sole engineering magnet school in Huntsville, Alabama. MSFC awarded the grant in hopes of fulfilling its goal of giving back invaluable resources to its community and engineers, as well as educating tomorrow's work force in the high-tech area of science and technology. Marshall engineers, Lee High School students and teachers, and a host of other volunteers and parents officially initiated this robot design process and competitive strategic game plan. The FIRST Robotics Competition is a national engineering contest, which immerses high school students in the exciting world of science and engineering. Teaming with engineers from government agencies, businesses, and universities enables the students to learn about the engineering profession. The students and engineers have six weeks to work together to brainstorm, design, procure, construct, and test their robot. The team then competes in a spirited, 'no-holds barred' tournament, complete with referees, other FIRST-designed robots, cheerleaders, and time clocks. The partnerships developed between schools, government agencies, businesses, and universities provide an exchange of resources and talent that build cooperation and expose students to new and rewarding career options. The result is a fun, exciting, and stimulating environment in which all participants discover the important connections between classroom experiences and real-world applications. This paper will highlight the story, engineering development, and evolutionary design of Xtraktor, the rookie robot, a manufacturing marvel and engineering achievement.

Henderson, A. J., Jr.↗

Plant Research

The Land's agricultural research team is testing new ways to sustain life in space as a research participant with Kennedy Space Center's Controlled Ecological Life Support System (CELSS). The Land, sponsored by Kraft General Foods, is an entertainment, research, and education facility at EPCOT Center, part of Walt Disney World. The cooperative effort is simultaneously a research and development program, a technology demonstration that provides the public to see high technology at work and an area of potential spinoff: the CELSS work may generate Earth use technology beneficial to the hydroponic (soilless growing) vegetable production industries of the world.

Source record↗

Next Generation Active Buffet Suppression System

Buffeting is an aeroelastic phenomenon that is common to high performance aircraft, especially those with twin vertical tails like the F/A-18, at high angles of attack. These loads result in significant random stresses, which may cause fatigue damage leading to restricted capabilities and availability of the aircraft. This paper describes an international collaborative research activity among Australia, Canada and the United States involving the use of active structural control to alleviate the damaging structural response to these loads. The research program is being co-ordinated by the Air Force Research Laboratory (AFRL) and is being conducted under the auspices of The Technical Cooperative Program (TTCP). This truly unique collaborative program has been developed to enable each participating country to contribute resources toward a program that coalesces a broad range of technical knowledge and expertise into a single investigation. This collaborative program is directed toward a full-scale test of an F/A-18 empennage, which is an extension of an earlier initial test. The current program aims at applying advanced directional piezoactuators, the aircraft rudder, switch mode amplifiers and advanced control strategies on a full-scale structure to demonstrate the enhanced performance and capability of the advanced active BLA control system in preparation for a flight test demonstration.

Galea, Stephen C.↗

Human Factors Evaluations of Two-Dimensional Spacecraft Conceptual Layouts

Much of the human factors work done in support of the NASA Constellation lunar program has been with low fidelity mockups. These volumetric replicas of the future lunar spacecraft allow researchers to insert test subjects from the engineering and astronaut population and evaluate the vehicle design as the test subjects perform simulations of various operational tasks. However, lunar outpost designs must be evaluated without the use of mockups, creating a need for evaluation tools that can be performed on two-dimension conceptual spacecraft layouts, such as floor plans. A tool based on the Cooper- Harper scale was developed and applied to one lunar scenario, enabling engineers to select between two competing floor plan layouts. Keywords: Constellation, human factors, tools, processes, habitat, outpost, Net Habitable Volume, Cooper-Harper.

Kennedy, Kriss J.↗

Insect-Inspired Flight Control for Unmanned Aerial Vehicles

Flight-control and navigation systems inspired by the structure and function of the visual system and brain of insects have been proposed for a class of developmental miniature robotic aircraft called "biomorphic flyers" described earlier in "Development of Biomorphic Flyers" (NPO-30554), NASA Tech Briefs, Vol. 28, No. 11 (November 2004), page 54. These form a subset of biomorphic explorers, which, as reported in several articles in past issues of NASA Tech Briefs ["Biomorphic Explorers" (NPO-20142), Vol. 22, No. 9 (September 1998), page 71; "Bio-Inspired Engineering of Exploration Systems" (NPO-21142), Vol. 27, No. 5 (May 2003), page 54; and "Cooperative Lander-Surface/Aerial Microflyer Missions for Mars Exploration" (NPO-30286), Vol. 28, No. 5 (May 2004), page 36], are proposed small robots, equipped with microsensors and communication systems, that would incorporate crucial functions of mobility, adaptability, and even cooperative behavior. These functions are inherent to biological organisms but are challenging frontiers for technical systems. Biomorphic flyers could be used on Earth or remote planets to explore otherwise difficult or impossible to reach sites. An example of an exploratory task of search/surveillance functions currently being tested is to obtain high-resolution aerial imagery, using a variety of miniaturized electronic cameras. The control functions to be implemented by the systems in development include holding altitude, avoiding hazards, following terrain, navigation by reference to recognizable terrain features, stabilization of flight, and smooth landing. Flying insects perform these and other functions remarkably well, even though insect brains contains fewer than 10(exp -4) as many neurons as does the human brain. Although most insects have immobile, fixed-focus eyes and lack stereoscopy (and hence cannot perceive depth directly), they utilize a number of ingenious strategies for perceiving, and navigating in, three dimensions. Despite their lack of stereoscopy, insects infer distances to potential obstacles and other objects from image motion cues that result from their own motions in the environment. The concept of motion of texture in images as a source of motion cues is denoted generally as the concept of optic or optical flow. Computationally, a strategy based on optical flow is simpler than is stereoscopy for avoiding hazards and following terrain. Hence, this strategy offers the potential to design vision-based control computing subsystems that would be more compact, would weigh less, and would demand less power than would subsystems of equivalent capability based on a conventional stereoscopic approach.

Thakoor, Sarita↗

Findings on Subtask 3.1 - Bakken Rich Gas Enhanced Oil Recovery Project

Total in-place oil for the Bakken petroleum system (BPS) (which includes the Bakken and Three Forks Formations) has been estimated to be 600 billion barrels (bbl). However, BPS wells have decline rates as high as 85% over the first 3 years of their lives, and primary recovery factors typically range from 3% to 10% of original oil in place. Given the low initial recovery rates, even small incremental productivity improvements could dramatically increase technically recoverable oil in the BPS. One potential solution is enhanced oil recovery (EOR) using gas injection, such as carbon dioxide (CO2) or hydrocarbon (HC) gases. While commonly used in conventional reservoirs, CO2 EOR in unconventional tight oil reservoirs has been limited to pilot tests. EOR using rich gas (mixture of methane, ethane, and propane) has also been employed in numerous pilots in several unconventional plays and has recently been successfully applied in the Eagle Ford play. If successful, large-scale gas-based EOR in the BPS could dramatically increase oil productivity and recovery factors and extend the life of the play for decades. While CO2 may be a technically suitable working fluid for EOR in the BPS, supplies are limited and costs for using CO2 in EOR pilots are prohibitively high. Meanwhile, produced gas flaring has presented challenges for BPS operators in North Dakota. Analysis conducted by the North Dakota Pipeline Authority indicates that the current gas-gathering infrastructure in North Dakota is insufficient to accommodate all of the associated gas that is produced from the BPS. The geographically isolated location of North Dakota relative to large natural gas markets, combined with suppressed natural gas prices, has made it economically challenging for industry to invest capital in expanding gas-gathering infrastructure in the state. These circumstances led to a research program conducted by the Energy & Environmental Research Center (EERC) in partnership with Liberty Resources Management Company LLC (LR) to examine the potential to use rich gas injection for EOR and mitigate flaring. A rich gas EOR pilot test was designed and executed by LR at its Stomping Horse development area in Williams County, North Dakota. From July 2018 through May 2019, a total of 160 million standard cubic feet (MMscf) of rich produced gas was injected into the BPS using five different wells in a sequential injection strategy. LR’s Leon–Gohrick drill spacing unit (DSU) was used as the test site. Regulatory oversight was provided by the North Dakota Industrial Commission (NDIC). Technical support was provided by the EERC through a series of laboratory, modeling, and field-based activities, and additional post-pilot research activities incorporated learnings from the test, developed new laboratory data, improved fracture modeling methods, and developed machine learning and big data analytics. The results from the Stomping Horse rich gas EOR pilot activities indicate that developing an effective, economical EOR approach for the BPS will require more field tests. Another key lesson learned from the Stomping Horse tests is that detailed pre- and posttest data on reservoir conditions and fluids production are essential. Robust reservoir characterization provides information that is crucial to creating realistic geomodels and conducting valid dynamic simulations of potential EOR scenarios. A detailed understanding of the completions and production history of offset wells is also necessary for valid test result interpretations. This knowledge is essential to designing the operational parameters of injectivity tests and interpreting the results. A conformance control strategy is also essential to success. Laboratory-based examinations of rich gas interactions with reservoir fluids and rocks were conducted, with an emphasis on determining the ability to mobilize oil in the tight reservoir rocks and shales of the BPS. Injection fluid composition was shown to have a positive impact on reducing reservoir oil minimum miscibility pressure (MMP), reducing interfacial tension (IFT), and altering wettability. IFT and contact angle measurements demonstrated that wettability can be altered in the presence of rich gas, suggesting the potential to improve oil recovery. Iterative modeling of surface infrastructure and reservoir performance using data generated by the various project activities was conducted. A geologic model of the Stomping Horse area was built; history-matched oil, gas, and water production was used in simulations of various EOR scenarios. Early programmatic modeling results were used to support LR’s design and operation of the EOR pilot and to provide insight regarding optimization of future commercial-scale BPS EOR design and operations. Post-pilot modeling focused on alternative methods of understanding complex fracture networks and accelerating simulation time. These led to improved simulation run times and provide excellent history-matching results. Several of these iterative models were used as the bases for developing algorithms into machine learning and big data analytics. History matching in reservoir simulation is time-consuming and computer processing-intensive. Machine learning algorithms were created, and an automated history-matching tool was developed. A large set of synthetic reservoir simulations were created to generate well responses (oil, gas, and water production, well bottomhole pressure [BHP], and tracer or propane breakthrough) for a set of EOR operating parameters that included offset well status (open or closed), injectate (rich gas or propane), injection rate, and injection well BHP. A user interface was developed to provide real-time visualization. Machine learning-based models were developed to provide rapid forecasting of well performance given a set of user-defined EOR operating parameters. These predictive models allow the user to modify the offset well status, injection rate, and injection well BHP and rapidly forecast future production performance. The combination of real-time visualization tools with real-time forecasting tools provides a framework for real-time control—operational changes that the EOR site operator can enact (e.g., changing gas injection rates) to affect the observed performance and potentially improve the EOR outcome. There is great reason to be optimistic about the future of EOR in the Bakken. The results of the laboratory studies suggest significant potential for high rates of oil mobilization using produced field gas injection under the right conditions. The results of the lab studies, combined with rigorous statistical analysis of well production data and associated modeling efforts, confirm the notion that fluid mobility within the reservoir is controlled by fractures. As more knowledge is gained about the nature and distribution of fracture networks in the Bakken, the industry will be in a better position to predict and, ultimately, influence fluid mobility. New field tests are necessary to develop a more complete understanding of those conditions. Thoughtful and creatively engineered field tests within a well-characterized geologic setting will yield the fundamental knowledge needed to take Bakken oil production to the next level. This subtask was cofunded through the EERC–U.S. Department of Energy Joint Program on Research and Development for Fossil Energy-Related Resources Cooperative Agreement No. DE-FE0024233. Nonfederal funding was provided by the North Dakota Industrial Commission’s Oil and Gas Research Program and Computer Modelling Group.

04 OIL SHALES AND TAR SANDS↗

Cooperative GN&C development in a rapid prototyping environment

The Navigation, Control and Aeronautics Division (NCAD) at NASA-JSC is exploring ways of producing Guidance, Navigation and Control (GN&C) flight software faster, better, and cheaper. To achieve these goals NCAD established two hardware/software facilities that take an avionics design project from initial inception through high fidelity real-time hardware-in-the-loop testing. Commercially available software products are used to develop the GN&C algorithms in block diagram form and then automatically generate source code from these diagrams. A high fidelity real-time hardware-in-the-loop laboratory provides users with the capability to analyze mass memory usage within the targeted flight computer, verify hardware interfaces, conduct system level verification, performance, acceptance testing, as well as mission verification using reconfigurable and mission unique data. To evaluate these concepts and tools, NCAD embarked on a project to build a real-time 6 DOF simulation of the Soyuz Assured Crew Return Vehicle flight software. To date, a productivity increase of 185 percent has been seen over traditional NASA methods for developing flight software.

Bordano, Aldo↗

Impact of Lateral Alignment on the Energy Savings of a Truck Platoon: Preprint

A truck platooning system was tested using two heavy-duty tractor-trailer trucks on a closed test track to investigate the sensitivity of intentional lateral offsets over a range of intervehicle spacings. The fuel consumption for both trucks in the platoon was measured using the SAE J1321 gravimetric procedure while travelling at 65 mph and loaded to a gross weight of 65,000 lb. In addition, the SAE J1939 instantaneous fuel rate was calibrated against the gravimetric measurements and used as proxy for additional analyses. The testing campaign demonstrated the effects of intervehicle gaps, following-vehicle longitudinal control, and manual lateral control. The new results are compared to previous truck-platooning studies to reinforce the value of the new information and demonstrate similarity to past trends. Fuel savings for the following vehicle was observed to exceed 10% at closer following distances. The results showed that energy savings generally increased in a non-linear fashion as the gap was reduced. The impact of different following-truck lateral offsets had a measurable impact, with up to 4% reduction in total fuel-savings (relative to an isolated vehicle condition) observed for offsets up to 1.3 m. The fuel-consumption savings on the straight segments of the track exceeded those on the curved segments by upwards of 6% and highlight some potential differences expected between close-track testing and on-highway use.

adaptive cruise control↗

Buster-Jangle

Buster-Jangle changed the working relationship between the AEC and the DoD. Beginning with Trinity and continuing through Greenhouse, the military was the logistical hub of nuclear testing, supplying men and material to carry out the myriad of mostly non-technical tasks. With the inclusion of the two Jangle tests and the Desert Rock exercises, that relationship became more symbiotic driven by the growing requirements for tactical weapons and their possible battlefield use. At Buster-Jangle, as well, the first use of a jet bomber was evidence that the military technology was evolving, requiring closer cooperation between the two agencies to match weapons and delivery systems.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Study of the structure of turbulent shear flows at supersonic speeds and high Reynolds number

A major effort to improve the accuracies of turbulence measurement techniques is described including the development and testing of constant temperature hot-wire anemometers which automatically compensate for frequency responses. Calibration and data acquisition techniques for normal and inclined wires operated in the constant temperature mode, flow geometries, and physical models to explain the observed behavior of flows are discussed, as well as cooperation with computational groups in the calculation of compression corner flows.

Smits, A. J.↗

The Space Homestead and Creation of Real Estate and Industry Beyond Earth

During the 1970's large habitats were proposed by G. K. O'Neill and studied by NASA that could house 10,000 to 4 million people in Earth/Moon space. These peoples would be employed in building space solar satellites and more habitats for new settlers. Such a program, the NASA studies concluded, could reach financial break even in 17 to 30 years of peak Apollo level expenditures. During the STAIF 2007 conference the first author presented a proposal to begin human settlement not by building city size structures but with a minimum technology habitat that could provide subsistence for a human family (10 people) and be capable of producing new habitats with extraterrestrial materials and energy. Such a habitat would be the equivalent of a space homestead. Later these habitats could cooperate to form towns and cities in a free ad hoe manner similar to the development of the American west. In addition the approach could provide a quicker return on investment and lower start up costs, and would be of a scale that could be developed and tested within the planned transportation and lunar base architecture of the Exploration Vision. This paper examines the population growth kinetics of humans in space, and the development of space solar power industry for the space homestead in comparison to larger habitat designs considered in the 1970's.

Curreri, Peter A.↗

Time-Varying Output Delay Compensation-A Model-Free Approach and its Application on Cooperative On-Ramp Merging

This paper presents a model-free approach to compensate for time-varying output delay in networked control systems. The proposed architecture combines a model-free observer and the Smith predictor. The model-free observer estimates the current state while handling modeling errors and uncertainties of the system. The Smith predictor moves the effect of time delay outside the control closed-loop using the estimated delayed output and the actual output of the plant. The proposed method is applied to a cooperative on-ramp merging problem. First, an ultra-local model predictive control is implemented to provide a computationally efficient online speed planner agnostic to the vehicle dynamics. After that, a model-free observer is designed to estimate the current state. Finally, the proposed architecture is tested against a time-varying output delay with an upper bound of 200 milliseconds. The results demonstrate the effectiveness of the proposed method with improved tracking of intervehicle distance.

Waleed khan, Muhammad [The University of Texas at ↗

Collection and analysis of remotely sensed data from the Rhode River Estuary Watershed

NASA chose the watershed of Rhode River, a small sub-estuary of the Bay, as a representative test area for intensive studies of remote sensing, the results of which could be extrapolated to other estuarine watersheds around the Bay. A broad program of ecological research was already underway within the watershed, conducted by the Smithsonian Institution's Chesapeake Bay Center for Environmental Studies (CBCES) and cooperating universities. This research program offered a unique opportunity to explore potential applications for remote sensing techniques. This led to a joint NASA-CBCES project with two basic objectives: to evaluate remote sensing data for the interpretation of ecological parameters, and to provide essential data for ongoing research at the CBCES. A third objective, dependent upon realization of the first two, was to extrapolate photointerpretive expertise gained at the Rhode River watershed to other portions of the Chesapeake Bay.

Jenkins, D. W.↗

Flight test evaluation of a video tracker for enhanced offshore airborne radar approach capability

As a part of NASA's Rotorcraft All-Weather Operations Research Program, advanced airborne radar approach (ARA) concepts are being investigated. Since data from previous NASA/FAA flight tests showed significant ARA limitations, a research program was initiated at NASA Ames Research Center to determine the benefit that could be derived by automating certain radar functions and superimposing course display data on the radar display. To evaluate these concepts, a newly developed video tracking system which interfaces with weather radar was acquired. After the pilot designates a destination target, the system tracks the target video as it moves on the radar indicator. Using a small, efficient microprocessor, the autotracker presents valuable approach data on the radar screen and automatically adjusts the radar gain and tilt. Results of a limited flight test evaluation of the autotracker show that the course display concept, combined with automated gain and tilt functions, is effective for improving ARA's and reducing radar operator workload.

Clary, G. R.↗

Pressure and heat transfer investigation of a modified NASP baseline configuration at M = 6

A cooperative NASA Langley-Boeing investigation was conducted in the Langley eight-Foot High Temperature Tunnel to obtain hypersonic pressure and heat transfer data. In this investigation a large scale (1/20), modified version of the National Aero-Space Plane configuration known as the 'Government Baseline' was tested at a nominal Mach number of 6; at two Reynolds numbers (0.6 and 1.6 million per foot); and at angles of attack from about 0 to 15 deg. There were several purposes for the investigation: to provide a windward and leeward pressure and heat transfer data base for a realistic configuration for verification of computational methods, to provide these data for a large-scale model, and to provide these data for true temperature conditions because of concern about data from low temperature tunnels.

Reubush, David E.↗