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Calcium and signal transduction in plants

Environmental and hormonal signals control diverse physiological processes in plants. The mechanisms by which plant cells perceive and transduce these signals are poorly understood. Understanding biochemical and molecular events involved in signal transduction pathways has become one of the most active areas of plant research. Research during the last 15 years has established that Ca2+ acts as a messenger in transducing external signals. The evidence in support of Ca2+ as a messenger is unequivocal and fulfills all the requirements of a messenger. The role of Ca2+ becomes even more important because it is the only messenger known so far in plants. Since our last review on the Ca2+ messenger system in 1987, there has been tremendous progress in elucidating various aspects of Ca(2+) -signaling pathways in plants. These include demonstration of signal-induced changes in cytosolic Ca2+, calmodulin and calmodulin-like proteins, identification of different Ca2+ channels, characterization of Ca(2+) -dependent protein kinases (CDPKs) both at the biochemical and molecular levels, evidence for the presence of calmodulin-dependent protein kinases, and increased evidence in support of the role of inositol phospholipids in the Ca(2+) -signaling system. Despite the progress in Ca2+ research in plants, it is still in its infancy and much more needs to be done to understand the precise mechanisms by which Ca2+ regulates a wide variety of physiological processes. The purpose of this review is to summarize some of these recent developments in Ca2+ research as it relates to signal transduction in plants.

NASA Program Space Biology↗

The Nature of the Flaring EUVE Companion to HD 43162

The purpose of our program was to observe and characterize the companion to HD 43162, EUVE J0614-2354, which (serendipitously) experienced an enormous flare event during our EUVE observation of HD 43162, one of the nearby solar analogs that we observed during our survey of this population. Our observation was carried out and the data have been received and reduced. We are able to identify EUVE J0614-2354 in both the X-ray (EPIC MOS + PN) and the UV (OM) data, which provides a sub-arcsecond position for this source. Our findings are consistent with the analysis of Christian et al. (2003a,b), who identify EUVE J0614-2354 with a coronally-active M-dwarf star at distance d = 15 plus or minus 5pc. The X-ray spectrum from the EPIC data are also consistent with this identification.

Kulkarni, Shrinivas R.↗

Identifying Indicators of Harmful Algal Blooms in Coastal Atacama Using Satellite Image Processing Techniques to Improve Industry and Authority Response

Over the past decade, Chile has experienced a significant rise in both the frequency and intensity of coastal Harmful Algal Blooms (HABs). These bloom events are a growing concern for the Atacama Region especially, with potential impacts on human health, aquaculture, and the environment. HABs are caused by an excess proliferation of microalgae, with certain algae species commonly found in Chilean HABs capable of producing toxins which can poison fish, toxify shellfish, and cause illness or death when ingested by humans. Collaborating with the Ministry of Health of Chile, Centro de Información de Recursos Naturales, the University of Atacama, and the Embassy of Chile’s Agricultural Office, this study identifies potential indicators of HABs in the Coastal Atacama Region using NASA Earth observations. Satellite imagery from the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument onboard NASA’s Aqua satellite was used for the identification of chlorophyll-a which provides direct estimates of the algae pigment’s concentration in water bodies. In addition, the spatiotemporal patterns of several other parameters were examined to identify correlations with occurrences of HABs, including sea surface temperature and normalized fluorescence line height using Aqua MODIS, Normalized Difference Turbidity Index (NDTI) using Landsat 8, and ocean wind speed measurements from the NOAA Joint Polar Satellite System. Analysis of these patterns revealed hotspots of high chlorophyll-a concentrations from the years 2014 to 2022, the most major of which occurring in the coastal zones of Chañaral, Bahía Inglesa, and Huasco. Also, through the use of a similarity search conducted, the seasonal variation of these indicators was mapped to highlight areas with great likelihood of experiencing HABs in the future. The results can guide future efforts for in-situ water sampling, toxin analysis and assessment, and HAB monitoring and prevention.

Cody O'Ferrall↗

Deep Learning Based Superconducting Radio-Frequency Cavity Fault Classification at Jefferson Laboratory

This work investigates the efficacy of deep learning (DL) for classifying C100 superconducting radio-frequency (SRF) cavity faults in the Continuous Electron Beam Accelerator Facility (CEBAF) at Jefferson Lab. CEBAF is a large, high-power continuous wave recirculating linac that utilizes 418 SRF cavities to accelerate electrons up to 12 GeV. Recent upgrades to CEBAF include installation of 11 new cryomodules (88 cavities) equipped with a low-level RF system that records RF time-series data from each cavity at the onset of an RF failure. Typically, subject matter experts (SME) analyze this data to determine the fault type and identify the cavity of origin. This information is subsequently utilized to identify failure trends and to implement corrective measures on the offending cavity. Manual inspection of large-scale, time-series data, generated by frequent system failures is tedious and time consuming, and thereby motivates the use of machine learning (ML) to automate the task. This study extends work on a previously developed system based on traditional ML methods (Tennant and Carpenter and Powers and Shabalina Solopova and Vidyaratne and Iftekharuddin, Phys. Rev. Accel. Beams, 2020, 23, 114601), and investigates the effectiveness of deep learning approaches. The transition to a DL model is driven by the goal of developing a system with sufficiently fast inference that it could be used to predict a fault event and take actionable information before the onset (on the order of a few hundred milliseconds). Because features are learned, rather than explicitly computed, DL offers a potential advantage over traditional ML. Specifically, two seminal DL architecture types are explored: deep recurrent neural networks (RNN) and deep convolutional neural networks (CNN). We provide a detailed analysis on the performance of individual models using an RF waveform dataset built from past operational runs of CEBAF. In particular, the performance of RNN models incorporating long short-term memory (LSTM) are analyzed along with the CNN performance. Furthermore, comparing these DL models with a state-of-the-art fault ML model shows that DL architectures obtain similar performance for cavity identification, do not perform quite as well for fault classification, but provide an advantage in inference speed.

97 MATHEMATICS AND COMPUTING↗

Preliminary input to the space shuttle reaction control subsystem failure detection and identification software requirements (uncontrolled)

The current baseline method and software implementation of the space shuttle reaction control subsystem failure detection and identification (RCS FDI) system is presented. This algorithm is recommended for conclusion in the redundancy management (RM) module of the space shuttle guidance, navigation, and control system. Supporting software is presented, and recommended for inclusion in the system management (SM) and display and control (D&C) systems. RCS FDI uses data from sensors in the jets, in the manifold isolation valves, and in the RCS fuel and oxidizer storage tanks. A list of jet failures and fuel imbalance warnings is generated for use by the jet selection algorithm of the on-orbit and entry flight control systems, and to inform the crew and ground controllers of RCS failure status. Manifold isolation valve close commands are generated in the event of failed on or leaking jets to prevent loss of large quantities of RCS fuel.

Bergmann, E.↗

An Analysis of the Origin and Propagation of the Multiple Coronal Mass Ejections of 2010 August 1

On 2010 August 1, the northern solar hemisphere underwent significant activity that involved a complex set of active regions near central meridian with, nearby, two large prominences and other more distant active regions. This activity culminated in the eruption of four major coronal mass ejections (CMEs), effects of which were detected at Earth and other solar system bodies. Recognizing the unprecedented wealth of data from the wide range of spacecraft that were available-providing the potential for us to explore methods for CME identification and tracking, and to assess issues regarding onset and planetary impact-we present a comprehensive analysis of this sequence of CMEs.We show that, for three of the four major CMEs, onset is associated with prominence eruption, while the remaining CME appears to be closely associated with a flare. Using instrumentation on board the Solar Terrestrial Relations Observatory spacecraft, three of the CMEs could be tracked out to elongations beyond 50◦; their directions and speeds have been determined by various methods, not least to assess their potential for Earth impact. The analysis techniques that can be applied to the other CME, the first to erupt, are more limited since that CME was obscured by the subsequent, much faster event before it had propagated far from the Sun; we discuss the speculation that these two CMEs interact. The consistency of the results, derived from the wide variety of methods applied to such an extraordinarily complete data set, has allowed us to converge on robust interpretations of the CME onsets and their arrivals at 1 AU.

Harrison, R. A.↗

Non-Nuclear Test Facility Development to Support Verification and Validation Needs

This study focuses on developing a non-nuclear test facility to help meet the verification and validation (V&V) requirements of a water-cooled small modular reactor (SMR) system. The scaling analysis necessary for reactor system experimentation and assessment model V&V considers various reactor system accident scenarios such as main steam line break (MSLB), steam generator tube rupture (SGTR), and containment condensation and heat transfer. The goal is to identify key physics based phenomena of interest (POI), specific figures of merit (FOMs), and the state of knowledge (SOK) by employing phenomena identification and ranking table (PIRT) studies. Important physics phenomena such as heat transfer, fluid dynamics, structural integrity, and material selection must be addressed when scaling a thermal-hydraulics system. Alongside these considerations, modifications to the facility may be needed to align the scaled system with the available footprint (height and space) of the facility. These modifications may include infrastructure upgrades, changes to the piping layout, implementation of safety measures, and installation of new instrumentation and control systems. The test facility aims to provide a scaled-down version of prototypic facilities for comprehensive V&V activities by overcoming these challenges and making the necessary modifications. This approach minimizes scaling distortion, simulates prototypic plant conditions, and accurately models accident event progressions. Thus, the facility will be capable of adequately modeling a scaled SMR system to support the design, development and licensing needs.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Localized keyhole pore prediction during laser powder bed fusion via multimodal process monitoring and X-ray radiography

Systematic fault detection and control during laser powder bed fusion (L-PBF) has been a long-standing objective for system manufacturers and researchers in the additive manufacturing (AM) industry. This manuscript investigates a data fusion approach for detection of keyhole porosity formation during laser irradiation of Ti-6Al-4V substrates by concurrent recording of thermally induced optical emission measured using both off-axis and coaxial photodiode sensors, and acoustic emission. Subsurface defect formation was monitored via high-speed synchrotron X-ray imaging at 20,000 frames per second, enabling temporal registration of keyhole pore formation events to the monitoring signals at a resolution of 50 µs. We developed data fusion machine learning (ML) models for localized prediction of keyhole pore formation at various time scales ranging from 0.5 ms to 2 ms. The signal segments were featurized using two independent approaches: (1) power spectral density (PSD) and (2) highly comparative time series analysis (HCTSA) framework. The extracted features from different sensor modalities were fused together to construct a multimodal feature space and sequential feature selection was used to determine the most informative features for training the ML models. The predictive performance was evaluated for three classifying algorithms: Support Vector Machine (SVM), K-Nearest Neighbor (KNN), and Gaussian Naive Bayes (GNB). As a result, pore formation events were predicted with up to 0.95 F1-score, 1.0 recall and 0.94 accuracy. The most heavily weighted features indicate that model performance is chiefly governed by the acoustic monitoring signal, with a secondary contribution from the optical emission sensors.

36 MATERIALS SCIENCE↗

Analyzing acoustic emission data to identify cracking modes in cement paste using an artificial neural network

This research is focused on the identification of cracking mechanisms for cement paste using acoustic emission data, recorded from compression and notched four-point bending tests. A procedure is developed for analyzing the data by employing an agglomerative hierarchical clustering method, an artificial neural network, and a ray-tracing source location algorithm. An agglomerative hierarchical clustering method is utilized to cluster the AE data from a compression test using frequency-dependent features. A neural network is trained using the compression test data and applied to the AE data emitted during the four-point bending test. The clustered data from the four-point bending test is localized using a ray-tracing algorithm. Based on the occurrence and locations of the clustered events and signal feature analyses, potential cracking mechanisms are identified and assigned.

36 MATERIALS SCIENCE↗

Observation of a threshold cusp at the Λ η threshold in the pK - mass spectrum with Λ$^{+}_{c}$ → pK - π + decays

We observe a narrow peaking structure in the pK - invariant-mass spectrum near the Λη threshold. The peak is clearly seen in 1.5 million events of Λ$^{+}_{c}$ → pK - π + decay using the 980 fb -1 data sample collected by the Belle detector at the KEKB asymmetric-energy e + e - collider. We try two approaches to explain this structure: as a new resonance and as a cusp at the Λη threshold. The best fit is obtained with a coherent sum of a Flatte function and a constant background amplitude with the reduced X 2 of 257/243 (p = 0.25), while the fits to Breit-Wigner functions are unfavored by more than 7σ. The best fit explains the structure as a cusp at the Λη threshold, and the obtained parameters are consistent with the known properties of Λ(1670). The observation gives the first identification of a threshold cusp in hadrons from the spectrum shape.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

AVIRIS performance during the 1987 flight season: An AVIRIS project assessment and summary of the NASA-sponsored performance evaluation

Results are presented of the assessment of AVIRIS performance during the 1987 flight season by the AVIRIS project and the earth scientists who were chartered by NASA to conduct an independent data quality and sensor performance evaluation. The AVIRIS evaluation program began in late June 1987 with the sensor meeting most of its design requirements except for signal-to-noise ratio in the fourth spectrometer, which was about half of the required level. Several events related to parts failures and design flaws further reduced sensor performance over the flight season. Substantial agreement was found between the assessments by the project and the independent investigators of the effects of these various factors. A summary of the engineering work that is being done to raise AVIRIS performance to its required level is given. In spite of degrading data quality over the flight season, several exciting scientific results were obtained from the data. These include the mapping of the spatial variation of atmospheric precipitable water, detection of environmentally-induced shifts in the spectral red edge of stressed vegetation, detection of spectral features related to pigment, leaf water and ligno-cellulose absorptions in plants, and the identification of many diagnostic mineral absorption features in a variety of geological settings.

Vane, Gregg↗

Achieving Maximum Integration Utilizing Requirements Flow Down

A robust and experienced systems engineering team is essential for a successful program. It is often a challenge to build a core systems engineering team early enough in a program to maximize integration and assure a common path for all supporting teams in a project. Ares I was no exception. During the planning of IVGVT, the team had many challenges including lack of: early identification of stakeholders, team training in NASA s system engineering practices, solid requirements flow down and a top down documentation strategy. The IVGVT team started test planning early in the program before the systems engineering framework had been matured due to an aggressive schedule. Therefore the IVGVT team increased their involvement in the Constellation systems engineering effort. Program level requirements were established that flowed down to IVGVT aligning all stakeholders to a common set of goals. The IVGVT team utilized the APPEL REQ Development Management course providing the team a NASA focused model to follow. The IVGVT team engaged directly with the model verification and validation process to assure that a solid set of requirements drove the need for the test event. The IVGVT team looked at the initial planning state, analyzed the current state and then produced recommendations for the ideal future state of a wide range of systems engineering functions and processes. Based on this analysis, the IVGVT team was able to produce a set of lessons learned and to provide suggestions for future programs or tests to use in their initial planning phase.

Archiable, Wes↗

Detecting Risk and Anomalies in Airplane Dynamics Through Entropic Analysis of Time Series Data

Despite recent efforts to move away from traditional threshold exceedance detection methods for aircraft state monitoring, modern aircraft still rely on safety thresholds to communicate to pilots the identification of an anomaly in the aircraft when a threshold is surpassed. Current anomaly detection methods mainly depend on uninterpretable machine learning models to learn complex patterns and relationships contained in the time series data of aircraft. Although these methods are capable of identifying known anomalies, their deficiency in interpretability presents a challenge when translating them to different aircraft. To overcome this deficiency, entropic analysis of aircraft dynamics seeks to characterize the complexity, or lack thereof, of the aircraft dynamics prior to the development of a risk scenario. This complexity characterization provides a more straightforward summary of state changes in the dynamics of flight variables. To build a foundation for entropic analysis, we analyzed the complexity of unstable approaches, an anomalous event present in many of today’s aviation accidents. The analysis revealed a statistically significant difference in the complexity distribution of flight variables under a stable approach versus an unstable approach. These differences in complexity were especially notable minutes before an approach was identified as unstable. Moreover, the multiscale entropic analysis revealed the presence of signal complexity at multiple time scales across multiple time windows before landing. By capturing state changes and corrections in the aircraft dynamics using entropy, advanced, yet still interpretable, sensor systems based on entropic frameworks from this study can be constructed in the future using classical machine learning approaches.

Risk detection↗

Qualitative Future Safety Risk Identification an Update

The purpose of this report is to document the results of a high-level qualitative study that was conducted to identify future aviation safety risks and to assess the potential impacts to the National Airspace System (NAS) of NASA Aviation Safety research on these risks. Multiple external sources (for example, the National Transportation Safety Board, the Flight Safety Foundation, the National Research Council, and the Joint Planning and Development Office) were used to develop a compilation of future safety issues risks, also referred to as future tall poles. The primary criterion used to identify the most critical future safety risk issues was that the issue must be cited in several of these sources as a safety area of concern. The tall poles in future safety risk, in no particular order of importance, are as follows: Runway Safety, Loss of Control In Flight, Icing Ice Detection, Loss of Separation, Near Midair Collision Human Fatigue, Increasing Complexity and Reliance on Automation, Vulnerability Discovery, Data Sharing and Dissemination, and Enhanced Survivability in the Event of an Accident.

future aviation safety risk↗

Time-dependent experimental identification of inter-ELM microtearing modes in the tokamak edge on DIII-D

In a series of discharges on the DIII-D tokamak, fast vertical plasma jogs are used to induce current perturbations in the steep gradient region of the H-mode edge. These current perturbations directly influence the edge q profile, decoupling the resonant location and instability drive of pedestal-localized microtearing modes (MTMs.) By exploiting this effect, we develop and apply a new experimental technique to track the dynamical frequency evolution of MTMs in the pedestal region, providing a compelling validation of the MTM model. The frequency of potential MTMs is calculated as the Doppler-shifted electron diamagnetic frequency at rational q=m/n surfaces, showing remarkable agreement with chirped frequency behavior of n=3, 4 and 5 modes detected with fast magnetics. Data is collected throughout multiple ELM cycles in order to build robust statistics describing the time-dependent frequency evolution of MTMs, which can be explained by examining the recovery of pedestal gradients after an ELM event. MTMs have a dominant transport contribution in the electron thermal channel, so the presented results indicate that reduced models of pedestal transport must be electromagnetic in nature and constructed with accurate calculations of MTM stability; inclusion of this physics is essential for accurate predictions of the electron temperature pedestal profile. Supporting measurements of mode saturation, propagation direction and transport fingerprints are made to support the dynamic frequency determination, unambiguously and experimentally identifying MTMs in the pedestal region of DIII-D.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Elucidating the differences in oxidation of high-performance α- and β- diisobutylene biofuels via Synchrotron photoionization mass spectrometry

Abstract Biofuels are a promising ecologically viable and renewable alternative to petroleum fuels, with the potential to reduce net greenhouse gas emissions. However, biomass sourced fuels are often produced as blends of hydrocarbons and their oxygenates. Such blending complicates the implementation of these fuels in combustion applications. Variations in a biofuel’s composition will dictate combustion properties such as auto ignition temperature, reaction delay time, and reaction pathways. A handful of novel drop-in replacement biofuels for conventional transportation fuels have recently been down selected from a list of over 10,000 potential candidates as part of the U.S. Department of Energy’s (DOE) Co-Optimization of Fuels and Engines (Co-Optima) initiative. Diisobutylene (DIB) is one such high-performing hydrocarbon which can readily be produced from the dehydration and dimerization of isobutanol, produced from the fermentation of biomass-derived sugars. The two most common isomers realized, from this process, are 2,4,4-trimethyl-1-pentene (α-DIB) and 2,4,4-trimethyl-2-pentene (β-DIB). Due to a difference in olefinic bond location, the α- and β- isomer exhibit dramatically different ignition temperatures at constant pressure and equivalence ratio. This may be attributed to different fragmentation pathways enabled by allylic versus vinylic carbons. For optimal implementation of these biofuel candidates, explicit identification of the intermediates formed during the combustion of each of the isomers is needed. To investigate the combustion pathways of these molecules, tunable vacuum ultraviolet (VUV) light (in the range 8.1–11.0 eV) available at the Lawrence Berkeley National Laboratory’s Advanced Light Source (ALS) has been used in conjunction with a jet stirred reactor (JSR) and time-of-flight mass spectrometry to probe intermediates formed. Relative intensity curves for intermediate mass fragments produced during this process were obtained. Several important unique intermediates were identified at the lowest observable combustion temperature with static pressure of 93,325 Pa and for 1.5 s residence time. As this relatively short residence time is just after ignition, this study is targeted at the fuels’ ignition events. Ignition characteristics for both isomers were found to be strongly dependent on the kinetics of C 4 and C 7 fragment production and decomposition, with the tert-butyl radical as a key intermediate species. However, the ignition of α-DIB exhibited larger concentrations of C 4 compounds over C 7 , while the reverse was true for β-DIB. These identified species will allow for enhanced engineering modeling of fuel blending and engine design.

09 BIOMASS FUELS↗

Synoptic/planetary-scale interactions and blocking over the North Atlantic Ocean

Work was completed on the height tendency diagnoses of two extratropical cyclones that occurred upstream from the blocking event studied previously. One developed explosively over water 60 to 36 hours before the block first appeared, while the second developed explosively over the southeastern United States during the time of block formation. In both cases, both vorticity and temperature advection were consistently important forcing mechanisms. This is in contrast to the block itself, in which vorticity advection was easily the dominant forcing mechanism. Latent heat release was also significant, accounting for about 50 percent of the total height falls in the cyclone below 850 mb. Estimates of latent heat release were greatly enhanced by coupling parameterized estimates with values derived from GOES IR data using an algorithm developed by Marshall's F. R. Robertson. Among the difficulties encountered in this work was the identification of an appropriate lower boundary condition for the solution of the height tendency equation. The zero value currently used tends to yield underestimates of the lower troposphere height tendencies. To address this problem a new diagnostic technique was developed in cooperation with Dr. Peter Zwack of the University of Quebec at Montreal. Based on an equation Dr. Zwack had previously developed (the Zwack-Okossi development equation), researchers now have a relationship that is completely consistent with the height tendency equation and provides estimates of lower boundary geostrophic vorticity or height tendencies. Finally, comparison of the SAT (satellite data) and NOSAT (no satellite data) analyses is progressing well. The present focus is on both the new diagnostic technique and the SAT/NOSAT comparisons. The former is being tested on the southeastern United States cyclone case previously mentioned and compared with the height tendency diagnoses already completed. The latter are being examined for the blocking case described in the publications cited in this summary. In addition to obtaining statistics that will allow general comparison of the two analyses, it will be possible to determine whether conclusions about the dynamics of the block development are influenced by the analysis set used.

Smith, Phillip J.↗

Solar Flare Catalog for SPICE Instrument on the Solar Orbiter

Studying the solar corona, the outermost layer of solar atmosphere, is a pivotal part of understanding the dynamic relations between solar activity and the solar wind, which can disrupt the near-Earth environment. Solar flares emit electromagnetic radiation in the solar corona, capable of releasing large amounts of energy in a matter of minutes. Flares can also be associated with Coronal Mass Ejections (CMEs) and affect Earth’s ionosphere. One instrument that can be used to study flares is the Spectral Imaging of the Coronal Environment (SPICE) instrumentaboard the Solar Orbiter (SolO). SPICE is a high-resolution extreme ultraviolet stigmatic slit spectrometer that covers emission lines formed from the solar chromosphere to corona. Since SPICE is a stigmatic slit spectrometer, the instrument can only take in data from a small spatial area on the Sun at a time. Due to the fast and unpredictable nature of flare events, it can be difficult to determine if and when SPICE has observed a flare. For this reason, we have created a catalog of flares observed by SPICE. This catalog of observational data was assembledby cross referencing data between different solar missions, including data from SolO’s E xtreme Ultraviolet Imager (EUI) and Spectrometer Telescope for Imaging X-rays (STIX), Solar Dynamics Observatory’s Atmospheric Imaging Assembly (SDO/AIA) instrument, and the Geostationary Operational Environmental Satellite (GOES-R). Supplemental analysis of the SPICE solar flare data includes Gaussian line fitting for flares of particular interest. The catalog can be utilized to locate and study coronal loop structures and flare ribbons. This SPICE solar flare catalog and additional supplemental analysis allows for the ease of identification of useful SPICE spectral data and multi-instrument analysis in order to study solar flare activity. It will be open for use by the Solar Orbiter and broader Heliophysics communities.

Anneliese L. Schmidt↗