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At least 793 records · Page 44
All sourcesNASA NTRSDOE OSTI
DOE OSTI2017

Flux-Enabled Exploration of the Role of Sip1 in Galactose Yeast Metabolism

13 C metabolic flux analysis ( 13 C MFA) is an important systems biology technique that has been used to investigate microbial metabolism for decades. The heterotrimer Snf1 kinase complex plays a key role in the preference Saccharomyces cerevisiae exhibits for glucose over galactose, a phenomenon known as glucose repression or carbon catabolite repression. The SIP1 gene, encoding a part of this complex, has received little attention, presumably, because its knockout lacks a growth phenotype. We present a fluxomic investigation of the relative effects of the presence of galactose in classically glucose-repressing media and/or knockout of SIP1 using a multi-scale variant of 13 C MFA known as 2-Scale 13 C metabolic flux analysis (2S- 13 C MFA). In this study, all strains have the galactose metabolism deactivated (gal1Δ background) so as to be able to separate the metabolic effects purely related to glucose repression from those arising from galactose metabolism. The resulting flux profiles reveal that the presence of galactose in classically glucose-repressing conditions, for a CEN.PK113-7D gal1Δ background, results in a substantial decrease in pentose phosphate pathway (PPP) flux and increased flow from cytosolic pyruvate and malate through the mitochondria toward cytosolic branched-chain amino acid biosynthesis. These fluxomic redistributions are accompanied by a higher maximum specific growth rate, both seemingly in violation of glucose repression. Deletion of SIP1 in the CEN.PK113-7D gal1Δ cells grown in mixed glucose/galactose medium results in a further increase. Knockout of this gene in cells grown in glucose-only medium results in no change in growth rate and a corresponding decrease in glucose and ethanol exchange fluxes and flux through pathways involved in aspartate/threonine biosynthesis. Glucose repression appears to be violated at a 1/10 ratio of galactose-to-glucose. Based on the scientific literature, we may have conducted our experiments near a critical sugar ratio that is known to allow galactose to enter the cell. Additionally, we report a number of fluxomic changes associated with these growth rate increases and unexpected flux profile redistributions resulting from deletion of SIP1 in glucose-only medium.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2016

THE HAWAII SCUBA-2 LENSING CLUSTER SURVEY: NUMBER COUNTS AND SUBMILLIMETER FLUX RATIOS

We present deep number counts at 450 and 850 μ m using the SCUBA-2 camera on the James Clerk Maxwell Telescope. We combine data for six lensing cluster fields and three blank fields to measure the counts over a wide flux range at each wavelength. Thanks to the lensing magnification, our measurements extend to fluxes fainter than 1 mJy and 0.2 mJy at 450 μ m and 850 μ m, respectively. Our combined data highly constrain the faint end of the number counts. Integrating our counts shows that the majority of the extragalactic background light (EBL) at each wavelength is contributed by faint sources with L {sub IR} < 10{sup 12} L {sub ⊙}, corresponding to luminous infrared galaxies (LIRGs) or normal galaxies. By comparing our result with the 500 μ m stacking of K -selected sources from the literature, we conclude that the K -selected LIRGs and normal galaxies still cannot fully account for the EBL that originates from sources with L {sub IR} < 10{sup 12} L {sub ⊙}. This suggests that many faint submillimeter galaxies may not be included in the UV star formation history. We also explore the submillimeter flux ratio between the two bands for our 450 μ m and 850 μ m selected sources. At 850 μ m, we find a clear relation between the flux ratio and the observed flux. This relation can be explained by a redshift evolution, where galaxies at higher redshifts have higher luminosities and star formation rates. In contrast, at 450 μ m, we do not see a clear relation between the flux ratio and the observed flux.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2016

SYSTEMATIC UNCERTAINTIES IN THE SPECTROSCOPIC MEASUREMENTS OF NEUTRON STAR MASSES AND RADII FROM THERMONUCLEAR X-RAY BURSTS. III. ABSOLUTE FLUX CALIBRATION

Many techniques for measuring neutron star radii rely on absolute flux measurements in the X-rays. As a result, one of the fundamental uncertainties in these spectroscopic measurements arises from the absolute flux calibrations of the detectors being used. Using the stable X-ray burster, GS 1826–238, and its simultaneous observations by Chandra HETG/ACIS-S and RXTE /PCA as well as by XMM-Newton EPIC-pn and RXTE /PCA, we quantify the degree of uncertainty in the flux calibration by assessing the differences between the measured fluxes during bursts. We find that the RXTE /PCA and the Chandra gratings measurements agree with each other within their formal uncertainties, increasing our confidence in these flux measurements. In contrast, XMM-Newton EPIC-pn measures 14.0 ± 0.3% less flux than the RXTE /PCA. This is consistent with the previously reported discrepancy with the flux measurements of EPIC-pn, compared with EPIC MOS1, MOS2, and ACIS-S detectors. We also show that any intrinsic time-dependent systematic uncertainty that may exist in the calibration of the satellites has already been implicity taken into account in the neutron star radius measurements.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2017

Sunward-propagating Solar Energetic Electrons inside Multiple Interplanetary Flux Ropes

On 2013 December 2 and 3, the SEPT and STE instruments on board STEREO-A observed two solar energetic electron events with unusual sunward-directed fluxes. Both events occurred during a time interval showing typical signatures of interplanetary coronal mass ejections (ICMEs). The electron timing and anisotropies, combined with extreme-ultraviolet solar imaging and radio wave spectral observations, are used to confirm the solar origin and the injection times of the energetic electrons. The solar source of the ICME is investigated using remote-sensing observations and a three-dimensional reconstruction technique. In situ plasma and magnetic field data combined with energetic electron observations and a flux-rope model are used to determine the ICME magnetic topology and the interplanetary electron propagation path from the Sun to 1 au. Two consecutive flux ropes crossed the STEREO-A location and each electron event occurred inside a different flux rope. In both cases, the electrons traveled from the solar source to 1 au along the longest legs of the flux ropes still connected to the Sun. During the December 2 event, energetic electrons propagated along the magnetic field, while during the December 3 event they were propagating against the field. As found by previous studies, the energetic electron propagation times are consistent with a low number of field line rotations N < 5 of the flux rope between the Sun and 1 au. The flux rope model used in this work suggests an even lower number of rotations.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2017

Dependence of the Peak Fluxes of Solar Energetic Particles on CME 3D Parameters from STEREO and SOHO

We investigate the relationships between the peak fluxes of 18 solar energetic particle (SEP) events and associated coronal mass ejection (CME) 3D parameters (speed, angular width, and separation angle) obtained from SOHO , and STEREO-A / B for the period from 2010 August to 2013 June. We apply the STEREO CME Analysis Tool (StereoCAT) to the SEP-associated CMEs to obtain 3D speeds and 3D angular widths. The separation angles are determined as the longitudinal angles between flaring regions and magnetic footpoints of the spacecraft, which are calculated by the assumption of a Parker spiral field. The main results are as follows. (1) We find that the dependence of the SEP peak fluxes on CME 3D speed from multiple spacecraft is similar to that on CME 2D speed. (2) There is a positive correlation between SEP peak flux and 3D angular width from multiple spacecraft, which is much more evident than the relationship between SEP peak flux and 2D angular width. (3) There is a noticeable anti-correlation ( r = −0.62) between SEP peak flux and separation angle. (4) The multiple-regression method between SEP peak fluxes and CME 3D parameters shows that the longitudinal separation angle is the most important parameter, and the CME 3D speed is secondary on SEP peak flux.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

Estimating scalar turbulent fluxes with slow-response sensors in the stable atmospheric boundary layer

Conventional and recently developed approaches for estimating turbulent scalar fluxes under stable atmospheric conditions are evaluated, with a focus on gases for which fast sensors are not readily available. First, the relaxed eddy accumulation (REA) classical approach and a recently proposed mixing length parameterization, labeled A22, are tested against eddy-covariance computations. Using high-frequency measurements collected from two contrasting sites (the frozen tundra near Utqiaġvik, Alaska, and a sparsely vegetated grassland in Wendell, Idaho, during winter), it is shown that the REA and A22 models outperform the conventional Monin–Obukhov similarity theory (MOST) utilized widely to infer fluxes from mean gradients. Second, scenarios where slow trace gas sensors are the only viable option in field measurements are investigated using digital filtering applied to fast-response sensors to simulate their slow-response counterparts. With a filtered scalar signal, the observed filtered eddy-covariance fluxes are referred to here as large-eddy-covariance (LEC) fluxes. A virtual eddy accumulation (VEA) approach, akin to the REA model but not requiring a mechanical apparatus to separate the gas flows, is also formulated and tested. A22 outperforms VEA and LEC in predicting the observed unfiltered (total) eddy-covariance (EC) fluxes; however, VEA can still capture the LEC fluxes well. This finding motivates the introduction of a sensor response time correction into the VEA formulation to offset the effect of sensor filtering on the underestimated net averaged fluxes. The only needed parameter for this correction is the mean velocity at the instrument height, a surrogate of the advective timescale. The VEA approach is very suitable and simple to use with gas sensors of intermediate speed (∼ 0.5 to 1 Hz) and with conventional open- or closed-path setups.

58 GEOSCIENCES↗
DOE OSTI2023

Harmonising the land-use flux estimates of global models and national inventories for 2000–2020

As the focus of climate policy shifts from pledges to implementation, there is a growing need to track progress on climate change mitigation at the country level, particularly for the land-use sector. Despite new tools and models providing unprecedented monitoring opportunities, striking differences remain in estimations of anthropogenic land-use CO 2 fluxes between, on the one hand, the national greenhouse gas inventories (NGHGIs) used to assess compliance with national climate targets under the Paris Agreement and, on the other hand, the Global Carbon Budget and Intergovernmental Panel on Climate Change (IPCC) assessment reports, both based on global bookkeeping models (BMs). Recent studies have shown that these differences are mainly due to inconsistent definitions of anthropogenic CO 2 fluxes in managed forests. Countries assume larger areas of forest to be managed than BMs do, due to a broader definition of managed land in NGHGIs. Additionally, the fraction of the land sink caused by indirect effects of human-induced environmental change (e.g. fertilisation effect on vegetation growth due to increased atmospheric CO 2 concentration) on managed lands is treated as non-anthropogenic by BMs but as anthropogenic in most NGHGIs. We implement an approach that adds the CO 2 sink caused by environmental change in countries' managed forests (estimated by 16 dynamic global vegetation models, DGVMs) to the land-use fluxes from three BMs. This sum is conceptually more comparable to NGHGIs and is thus expected to be quantitatively more similar. Our analysis uses updated and more comprehensive data from NGHGIs than previous studies and provides model results at a greater level of disaggregation in terms of regions, countries and land categories (i.e. forest land, deforestation, organic soils, other land uses). Our results confirm a large difference (6.7 GtCO 2 yr —1 ) in global land-use CO 2 fluxes between the ensemble mean of the BMs, which estimate a source of 4.8 GtCO 2 yr —1 for the period 2000–2020, and NGHGIs, which estimate a sink of —1.9 GtCO 2 yr —1 in the same period. Most of the gap is found on forest land (3.5 GtCO 2 yr —1 ), with differences also for deforestation (2.4 GtCO 2 yr —1 ), for fluxes from other land uses (1.0 GtCO 2 yr —1 ) and to a lesser extent for fluxes from organic soils (0.2 GtCO 2 yr —1 ). By adding the DGVM ensemble mean sink arising from environmental change in managed forests (—6.4 GtCO 2 yr —1 ) to BM estimates, the gap between BMs and NGHGIs becomes substantially smaller both globally (residual gap: 0.3 GtCO 2 yr —1 ) and in most regions and countries. However, some discrepancies remain and deserve further investigation. For example, the BMs generally provide higher emissions from deforestation than NGHGIs and, when adjusted with the sink in managed forests estimated by DGVMs, yield a sink that is often greater than NGHGIs. In summary, this study provides a blueprint for harmonising the estimations of anthropogenic land-use fluxes, allowing for detailed comparisons between global models and national inventories at global, regional and country levels. This is crucial to increase confidence in land-use emissions estimates, support investments in land-based mitigation strategies and assess the countries' collective progress under the Global Stocktake of the Paris Agreement.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2018

The dependence of divertor power sharing on magnetic flux balance in near double-null configurations on Alcator C-Mod

Management of power exhaust will be a crucial task for tokamak fusion reactors. Reactor concepts are often proposed with double-null divertors, i.e. having two magnetic separatrices in an up-down symmetric configuration. This arrangement is potentially advantageous since the majority of the tokamak exhaust power tends to flow to the outer pair of divertor legs at large major radius, where the geometry is favorable for spreading the heat over a large surface area and there is more room for advanced divertor configurations. Despite the importance, there have been relatively few studies of divertor power sharing in near double null configurations and no studies at the poloidal magnetic fields and scrape-off layer power widths anticipated for a reactor. Motivated by this need we have undertaken a systematic study on Alcator C-Mod, examining the effect of magnetic flux balance on the power sharing among the four divertor legs in near double-null plasmas. Ohmic L-modes at three values of plasma current and ICRF-heated enhanced D-alpha (EDA) H-modes and I-modes at a single value of plasma current are explored, producing poloidal magnetic fields of 0.42, 0.62 and 0.85 Tesla. For Ohmic L-modes and ICRF-heated EDA H-modes, we find that the point of equal power sharing between upper and lower divertors occurs remarkably close to a balanced double null. Power sharing amongst the outer (upper versus lower) and inner (upper versus lower) pairs of divertors can be described in terms of a logistic function of magnetic flux balance, consistent with heat flux mapping along magnetic field lines to the outer midplane. Power sharing between inner and outer legs is found to follow a Gaussian-like function of magnetic flux balance with non-zero power to the inner divertors at double null. The overall behavior of H-modes operated near double null and for I-modes operating to within one heat flux e-folding of double null are found similar to Ohmic L-modes, with a significant reduction of power on the inner divertor legs. The results are encapsulated in terms of empirically-informed analytic functions of magnetic flux balance. When combined with magnetic equilibrium control system specifications, these relationships can be used to specify the power flux handling requirements for each of the four divertor target plates.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
NASA NTRS1971

Hydrogen density and proton flux in the topside ionosphere over Arecibo, Puerto Rico, from incoherent scatter observations.

Incoherent scatter observations of the topside ionosphere over Arecibo, Puerto Rico, have been analyzed and interpreted to give values for the neutral hydrogen density and vertical proton flux throughout a 30 hr period on December 7 and 8, 1965. The neutral hydrogen density is of the order of 1,000,000 per cu cm at 520 km, agreeing well with other recent measurements. A diurnal variation of about 2-1 was found, which confirms recent theoretical predictions. The vertical proton flux attained a maximum value of about one billion per sq cm per sec, being upward in the daytime and downward at night. The daytime flux appears to be of comparable magnitude with the limiting flux permitted, but the shape of the ion density profile suggests that the flux was not actually a limiting flux. For the night in question, the downward proton flux appears to account for the maintenance of the F layer, perhaps with some additional contribution from neutral winds and/or electric fields.

Ho, M. C.↗
NASA NTRS1985

Heat Flux Sensor Calibrator

The heat flux to space shuttle main engine (SSME) turbopump turbine blades may be as high as 10 to the 7th power. The heat flux causes thermal transients that are of the order of 1 sec as temperature varies from perhaps 1500 K to 100 K. It is suspected that these transients cause durability problems in the turbine blades. To quantitatively evaluate the effect of these transients, heat flux sensors or gauges were developed to obtain data to verify analytical models. The objective is to design and fabricate a system for steady state and transient calibration and durability testing of heat flux sensors for use in SSME turbine blades. The calibrator consists of: (1) the arc lamp, (2) a high speed positioning table for placing standard and special heat flux sensors in the incident beam of radiant heat flux, (3) provision for cooling the blade and special sensors inserted in the blade, (4) a computer for controlling the positioning table, storing electrical output values from sensors, and calculating heat flux from these values, and (5) a pyrometer for measuring sensor surface temperatures.

Liebert, C. H.↗
NASA NTRS1985

Latitude variation of recurrent fluxes in the outer solar system

Recurrent low energy (greater than or = to 0.5 MeV) proton flux enhancements, reliable indicators of corotating plasma interaction regions, were observed on the Voyager 1 and 2 and Pioneer 11 spacecraft in the heliographic latitude range 2 deg S to 23 N and the heliocentric radial range 11 to 20 AU. After a period of rather high correlation between fluxes at different latitudes in early 1983, distinct differences developed in the fluxes during an overall flux decrease. The flux intensities returned to higher levels in early 1984 and differences in both the recurrence frequency and flux intensity persisted into 1985, as Voyager 1 traveled to 23 AU and 25 N latitude. Intercomparison of data from the three spacecraft indicates that the flux differences are most likely due to latitudinal rather than radial or temporal variations.

Christon, S. P.↗
NASA NTRS1984

Measuring planetary neutron albedo fluxes by remote gamma-ray sensing

In order to measure the planetary neutron albedo fluxes, a neutron-absorbing shield which emits gamma rays of characteristic energy and serves as a neutron detector, is added to a gamma-ray spectrometer (GRS). The gamma rays representing the neutron flux are observed against interference consisting of cosmic gamma rays, planetary continuum and line emission, and gamma rays arising from the interaction of cosmic rays with the GRS and the spacecraft. The uncertainty and minimum detection limits in neutron albedo fluxes are calculated for two missions, a lunar orbiter and a comet nucleus rendezvous. A GRS on a lunar orbiter at 100 km altitude detects a thermal neutron albedo flux as low as 0.002/sq cm/s and an expected flux of about 0.6/sq cm/s is measured with an uncertainty of 0.001/sq cm/s, for a 100 h observation period. For the comet nucleus, again in a 100 h observing period, a thermal neutron albedo flux is detected at a level of 0.006/sq cm/s and an expected flux of about 0.4/sq cm/s is measured with an uncertainty of 0.004/sq cm/s. The expanded geological capabilities made possible by this technique include improvements in H sensitivity, spatial resolution, and measurement depth; and an improved model of induced gamma-ray emission.

Haines, E. L.↗
NASA NTRS1985

Vector representation of trade cumulus thermodynamic fluxes

A tephigram of BOMEX averaged thermodynamic flux vectors, developed from two tradewind budgets, is presented. The parametric model, which is utilized to represent the fluxes in the tradewind layer of the product as a mass flux and a cloud-environment difference, is examined. A table of mean structure and fluxes of a dataset for the BOMEX ship array, and the Bowen ratio and convective mass flux for a nonmixing and a cloud-top mixing model is provided. The fluxes which are displayed in a simple vector format, using the saturation point notation of Betts (1982) reveal a coupling of fluxes and the relationship between Bowen ratio and height. It is concluded that with good tradewind budget data about the statistics of cloud mixing and radiative-convective coupling can be expressed.

Betts, A. K.↗
NASA NTRS1985

The cancellation of magnetic flux. II - In a decaying active region

H-alpha filtergrams and videomagnetograms are used to study an active region during its period of decay on August 3-8, 1984; the decay had been initiated by a fragmentation process in which very small knots of magnetic flux separated from larger concentration of flux. The disappearance of magnetic flux was always observed when the small fragments of flux encountered other small fragments or concentrations of flux of opposite polarity. Such 'cancellations' are shared by both polarities of magnetic field, and it is deduced that the disappearance of flux occurred either at or within 5 arcsec of the apparent dividing line between the opposite polarities. All of the 22 flares observed during the decay of this region were initiated around sites where magnetic flux was cancelling or was deduced to be cancelling during the flares. It is hypothesized that cancellation was one of the necessary conditions for flaring in this active region.

Martin, S. F.↗
NASA NTRS1986

A statistical study of plasma sheet dynamics using ISEE 1 and 2 energetic particle flux data

Plasma sheet dynamics during substorms are studied by analyzing 461 cases of transient dropout events of the 1.5 and 6-keV particle fluxes detected by ISEE 1 and 2 satellites. The instruments for detecting low- and high-energy particles are described. The spatial distribution of flux dropout events, and the events' relationship to magnetospheric activity level are examined. Substorm events without observed flux dropout events are investigated. The data reveal that the flux dropout distribution is isotropic, between 12-23 earth radii, and is present in the entire nightside plasma sheet; and the substorms without flux dropout are more frequent near earth and magnetospheric flanks. It is observed that tailward of 12 earth radii the flux dropout events and substorms without flux dropout are similar. The Chao et al. (1977) MHD rarefaction wave propagation model and the Hones (1973, 1980) near-tail, X-type magnetic neutral line formation model are discussed and compared to the experimental data. It is noted that neither model explains the plasma sheet dynamics observed.

Dandouras, J.↗
NASA NTRS1987

Magnetic flux tubes as sources of wave generation

The structure of solar, and very likely stellar, surface magnetic fields is highly inhomogeneous: at the photospheric level, the fields are locally strong, and show concentration into a flux tube structure. In this case, the wave energy generated in stellar convection zones may be largely carried away by flux tube waves, which can then become important sources for the heating of the outer atmospheric layers. Such flux tube wave generation may help to explain the UV and X-ray fluxes observed by the IUE and Einstein observatories. The generation of longitudinal tube waves in magnetic flux tubes embedded in an otherwise magnetic field-free, turbulent, and stratified medium was considered. It is shown that compressible tube waves are generated by dipole emission and that the generation efficiency is a strong function of the magnetic field strength. Energy flux calculations are presented for different magnetic flux tubes, and show how the results depend on the magnetic field strength and the characteristics of the convective turbulence.

Musielak, Z. E.↗
NASA NTRS1989

Automated Heat-Flux-Calibration Facility

Computer control speeds operation of equipment and processing of measurements. New heat-flux-calibration facility developed at Lewis Research Center. Used for fast-transient heat-transfer testing, durability testing, and calibration of heat-flux gauges. Calibrations performed at constant or transient heat fluxes ranging from 1 to 6 MW/m2 and at temperatures ranging from 80 K to melting temperatures of most materials. Facility developed because there is need to build and calibrate very-small heat-flux gauges for Space Shuttle main engine (SSME).Includes lamp head attached to side of service module, an argon-gas-recirculation module, reflector, heat exchanger, and high-speed positioning system. This type of automated heat-flux calibration facility installed in industrial plants for onsite calibration of heat-flux gauges measuring fluxes of heat in advanced gas-turbine and rocket engines.

Liebert, Curt H.↗
NASA NTRS1988

Rotational modulation of hydrogen Lyman alpha flux from 44ii Bootis

Observations with IUE that cover the entire 6.4 hr orbital cycle of the late-type contact binary 44i Bootis are presented. Intrinsic stellar hydrogen Lyman alpha emission flux was determined from low-resolution IUE spectra, compensating for geocoronal emission and for interstellar absorption. The variation of the stellar Lyman alpha emission flux correlates well with the variation of the C II and C IV emission fluxes, and shows orbital modulation in phase with the visual light curve. The ratio of Lyman alpha to CII flux (15 to 20) is similar to that observed in solar active regions. Hydrogen Lyman alpha emission is thus one of the most important cooling channels in the outer atmosphere of 44i Boo. A high-resolution spectrum of the Lyman alpha line was obtained between orbital phases 0.0 and 0.6. The integrated flux in the observed high-resolution Lyman alpha profile is consistent with the fluxes determined using low-resolution spectra, and the composite profile indicates that both components of this binary have equally active chromospheres and transition regions. The uncertainty in the interstellar hydrogen column density cannot mimic the observed variation in the integrated Lyman alpha flux, because the stellar line is very much broader than the interstellar absorption.

Vilhu, O.↗