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NASA NTRS1994

Vertical Axis Rotational Motion Cues in Hovering Flight Simulation

A previous study that examined how yaw motion affected a pilot's ability to perform realistic hovering flight tasks indicated that any amount of pure yaw motion had little-to-no effect on pilot performance or opinion. In that experiment, pilots were located at the vehicle's center of rotation; thus lateral or longitudinal accelerations were absent. The purpose of the new study described here was to investigate further these unanticipated results for additional flight tasks, but with the introduction of linear accelerations associated with yaw rotations when the pilot is not at the center of rotation. The question of whether a yaw motion degree-of-freedom is necessary or not is important to government regulators who specify what simulator motions are necessary according to prescribed levels of simulator sophistication. Currently, specifies two levels of motion sophistication for flight simulators: full 6-degree-of-freedom and 3-degree-of-freedom. For the less sophisticated simulator, the assumed three degrees of freedom are pitch, roll, and heave. If other degrees of freedom are selected, which are different f rom these three, they must be qualified on a case-by-case basis. Picking the assumed three axes is reasonable and based upon experience, but little empirical data are available to support the selection of critical axes. Thus, the research described here is aimed at answering this question. The yaw and lateral degrees of freedom were selected to be examined first, and maneuvers were defined to uncouple these motions from changes in the gravity vector with respect to the pilot. This approach simplifies the problem to be examined. For this experiment, the NASA Ames Vertical Motion Simulator was used in a comprehensive investigation. The math model was an AH-64 Apache in hover, which was identified from flight test data and had previously been validated by several AH-64 pilots. The pilot's head was located 4.5 ft in front of the vehicle center of gravity, which is representative of the AH-64 pilot location. Six test pilots flew three tasks that were specifically designed to represent a broad class of situations in which both lateral and yaw motion cues may be useful. For the first task, the pilot controlled only the yaw axis and was required to rapidly acquire a North heading from 15 deg yaw offsets to either the East or West. This task allowed for full, or 1:1, motion to be used in all axes (yaw, lateral, and longitudinal). The second task was a 10 sec., 180 deg. pedal turn over a runway, but with the pilot only controlling the yaw degree-of-freedom. The position of the vehicle's center-of-mass remained fixed. This maneuver was taken from a current U.S. Army rotary wing design standard5 and is representative of a maneuver performed for acceptance of military helicopters; however, it does not allow for full 1:1 motion, since the simulator cab cannot rotate 180 deg. The third task required the pilot to perform a rapid 9 ft climb at a constant heading. This task was challenging, because rapid collective lever movement in the unaugmented AH64 results in a substantial yawing moment (due to engine torque) that must be countered by the pilot. This task also had full motion in all axes, but, in this case, the pilot had two axes to control simultaneously, rather than one as in the previous tasks. Four motion configurations were examined for each task: full motion (except for the 180 deg turn, for which the motion system was configured to provide as much motion as possible), full linear with no yaw motion, full yaw with no linear motion, and no motion. Each configuration was flown four times in a randomized test matrix, and the pilots were not informed of the configuration given. Vehicle state data were recorded for objective performance comparisons, and pilots provided subjective comments and ratings. As part of the pilots' evaluation, they were asked to rate the compensation required, the overall fidelity of the motion as compared to real flight, and whether motion was detected or not in each of the six degrees of freedom. In addition, the pilots provided a numerical level-of confidence rating, between 1 and 7, corresponding to how sure they were whether or not motion was present in each degree-of-freedom. The latter rating allow classical signal detection analysis to be performed.

Schroeder, Jeffrey A.↗

NASA NTRS1994

Large Eddy Simulation of Homogeneous Rotating Turbulence

Study of turbulent flows in rotating reference frames has proven to be one of the more challenging areas of turbulence research. The large number of theoretical, experimental, and computational studies performed over the years have demonstrated that the effect of solid-body rotation on turbulent flows is subtle and remains exceedingly difficult to predict. Because of the complexities associated with non-homogeneous turbulence, it is worthwhile to examine the effect of steady system rotation on the evolution of an initially isotropic turbulent flow. The assumption of statistical homogeneity considerably simplifies analysis and computation; calculation of homogeneous turbulence is further motivated since it possesses the essential physics found in more complex rotating flows. The principal objectives of the present study have therefore been to increase our fundamental understanding of turbulent flows in rotating reference frames through an examination of the asymptotic state of homogeneous rotating turbulence; particularly as to the existence of an asymptotic state which is self similar. Knowledge of an asymptotic similarity state permits prediction of the ultimate statistical evolution of the flow without requiring detailed knowledge of the complex, and not well understood, non-linear transfer processes. Aside from examination of possible similarity states in rotating turbulence, of further interest in this study has been an examination of the degree to which solid-body rotation induces a two-dimensional state in an initially isotropic flow.

Squires, Kyle D.↗

NASA NTRS2001

Wind-Stress Simulations and Equatorial Dynamics in an AGCM: Basic Results from a 1979-1999 Forced SST Experiment - Part 1

This is the first of a two part study examining the connection of the equatorial momentum budget in an AGCM (Atmospheric General Circulation Model), with simulated equatorial surface wind stresses over the Pacific. The AGCM used in this study forms part of a newly developed coupled forecasting system used at NASA's Seasonal- to-Interannual Prediction Project. Here we describe the model and present results from a 20-year (1979-1999) AMIP-type experiment forced with observed SSTs (Sea Surface Temperatures). Model results are compared them with available observational data sets. The climatological pattern of extra-tropical planetary waves as well as their ENSO-related variability is found to agree quite well with re-analysis estimates. The model's surface wind stress is examined in detail, and reveals a reasonable overall simulation of seasonal interannual variability, as well as seasonal mean distributions. However, an excessive annual oscillation in wind stress over the equatorial central Pacific is found. We examine the model's divergent circulation over the tropical Pacific and compare it with estimates based on re-analysis data. These comparisons are generally good, but reveal excessive upper-level convergence in the central Pacific. In Part II of this study a direct examination of individual terms in the AGCM's momentum budget is presented. We relate the results of this analysis to the model's simulation of surface wind stress.

Bacmeister, Julio T.↗

NASA NTRS2001

Latent Heating Retrievals Using the TRMM Precipitation Radar: A Multi-Seasonal Study

Rainfall is a key link in the hydrologic cycle and is a primary heat source for the atmosphere. The vertical distribution of latent heat release, which is accompanied by rainfall, modulates the large-scale circulations of the tropics and in turn can impact midlatitude weather. This latent heat release is a consequence of phase changes between vapor, liquid, and solid water. Present largescale weather and climate models can simulate latent heat release only crudely, thus reducing their confidence in predictions on both global and regional scales. This paper represents the first attempt to use NASA Tropical Rainfall Measuring Mission (TRMM) rainfall information to estimate the four-dimensional structure of global monthly latent heating profiles over the global tropics from December 1997 to October 2000. The Goddard Convective-Stratiform. Heating (CSH) algorithm and TRMM precipitation radar data are used for this study. We will examine and compare the latent heating structures between 1997-1998 (winter) ENSO and 1998-2000 (non-ENSO). We will also examine over the tropics. The seasonal variation of heating over various geographic locations (i.e., oceanic vs continental; Indian oceans vs west Pacific; Africa vs S. America) will be also examined and compared. In addition, we will examine the relationship between latent heating (max heating level) and SST. The period of interest also coincides with several TRMM field campaigns that recently occurred over the South China Sea in 1998 (SCSMEX), Brazil in 1999 (TRMM-LBA), and in the central Pacific in 1999 (KWAJEX). Sounding diagnosed Q1 budgets from these experiments could provide a means of validating the retrieved profiles of latent heating from the CSH algorithm.

Tao, Wei-Kuo↗

NASA NTRS1996

Evaluating Fatigue in Operational Settings: The NASA Ames Fatigue Countermeasures Program

In response to a 1980 Congressional request, NASA Ames initiated a program to examine fatigue in flight operations. The Program objectives are to examine fatigue, sleep loss, and circadian disruption in flight operations, determine the effects of these factors on flight crew performance, and the development of fatigue countermeasures. The NASA Ames Fatigue Countermeasures Program conducts controlled laboratory experiments, full-mission flight simulations, and field studies. A range of subjective, behavioral, performance, physiological, and environmental measures are used depending on study objectives. The Program has developed substantial expertise in gathering data during actual flight operations and in other work settings. This has required the development of ambulatory and other measures that can be carried throughout the world and used at 41,000 feet in aircraft cockpits. The NASA Ames Fatigue Countermeasures Program has examined fatigue in shorthaul, longhaul, overnight cargo, and helicopter operations. A recent study of planned cockpit rest periods demonstrated the effectiveness of a brief inflight nap to improve pilot performance and alertness. This study involved inflight reaction time/vigilance performance testing and EEG/EOG measures of physiological alertness. The NASA Ames Fatigue Countermeasures Program has applied scientific findings to the development of education and training materials on fatigue countermeasures, input to federal regulatory activities on pilot flight, duty, and rest requirements, and support of National Transportation Safety Board accident investigations. Current activities are examining fatigue in nonaugmented longhaul flights, regional/commuter flight operations, corporate/business aviation, and psychophysiological variables related to performance.

Rosekind, Mark R.↗

NASA NTRS1997

Empirical and Analytic Studies Human/Automation Dynamics in Airspace Management for Free Flight

NASA and the FAA have initiated programs of research and development to provide flight crew, airline operations and air traffic managers with automation aids to increase capacity in en route and terminal area to support the goals of free flight for safe, flexible, predictable, and efficient operations. To support the development of those aiding systems human performance in automated aiding has been examined in empirical and computationally analytic studies. This paper presents a set of those studies in full mission simulation and the development of a predictive computational model of human performance. We have found that the combination of methodologies provide a powerful design-aiding process. We will describe three research programs in support of Free Flight Operations from the perspective of human performance requirements. We have examined procedures and communications in the use of voice and data-link operation at the transition between unconstrained (enroute) and constrained (terminal) airspace operations. We have examined the timing and form of Center TRACON Automation System (CTAS) advisories in descent. We have investigated the shape and dimensions of an 'alert zone' for air-based separation in unconstrained operations. Finally, we have examined the interaction of a ground-based conflict detection/resolution aiding system, as it interacts with a cockpit-based conflict alerting mechanism. Additional information is contained in the original extended abstract.

Corker, Kevin M.↗

NASA NTRS2003

Statistical Distribution of Inflation on Lava Flows: Analysis of Flow Surfaces on Earth and Mars

The surface morphology of a lava flow results from processes that take place during the emplacement of the flow. Certain types of features, such as tumuli, lava rises and lava rise pits, are indicators of flow inflation or endogenous growth of a lava flow. Tumuli in particular have been identified as possible indicators of tube location, indicating that their distribution on the surface of a lava flow is a junction of the internal pathways of lava present during flow emplacement. However, the distribution of tumuli on lava flows has not been examined in a statistically thorough manner. In order to more rigorously examine the distribution of tumuli on a lava flow, we examined a discrete flow lobe with numerous lava rises and tumuli on the 1969 - 1974 Mauna Ulu flow at Kilauea, Hawaii. The lobe is located in the distal portion of the flow below Holei Pali, which is characterized by hummocky pahoehoe flows emplaced from tubes. We chose this flow due to its discrete nature allowing complete mapping of surface morphologies, well-defined boundaries, well-constrained emplacement parameters, and known flow thicknesses. In addition, tube locations for this Mauna Ulu flow were mapped by Holcomb (1976) during flow emplacement. We also examine the distribution of tumuli on the distal portion of the hummocky Thrainsskjoldur flow field provided by Rossi and Gudmundsson (1996). Analysis of the Mauna Ulu and Thrainsskjoldur flow lobes and the availability of high-resolution MOC images motivated us to look for possible tumuli-dominated flow lobes on the surface of Mars. We identified a MOC image of a lava flow south of Elysium Mons with features morphologically similar to tumuli. The flow is characterized by raised elliptical to circular mounds, some with axial cracks, that are similar in size to the tumuli measured on Earth. One potential avenue of determining whether they are tumuli is to look at the spatial distribution to see if any patterns similar to those of tumuli-dominated terrestrial flows can be identified. Since tumuli form by the injection of lava beneath a crust, the distribution of tumuli on a flow should represent the distribution of thermally preferred pathways beneath the surface of the crust. That distribution of thermally preferred pathways may be a function of the evolution of a basaltic lava flow. As a longer-lived flow evolves, initially broad thermally preferred pathways would evolve to narrower, more well-defined tube-like pathways. The final flow morphology clearly preserves the growth of the flow over time, with inflation features indicating pathways that were not necessarily contemporaneously active. Here, we test using statistical analysis whether this final flow morphology produces distinct distributions that can be used to readily determine the distribution of thermally preferred pathways beneath the surface of the crust.

Glazel, L. S.↗

NASA NTRS2003

Overview of Atmospheric Ionizing Radiation (AIR)

The SuperSonic Transport (SST) development program within the US was based at the Langley Research Center as was the Apollo radiation testing facility (Space Radiation Effects Laboratory) with associated radiation research groups. It was natural for the issues of the SST to be first recognized by this unique combination of research programs. With a re-examination of the technologies for commercial supersonic flight and the possible development of a High Speed Civil Transport (HSCT), the remaining issues of the SST required resolution. It was the progress of SST radiation exposure research program founded by T. Foelsche at the Langley Research Center and the identified remaining issues after that project over twenty-five years ago which became the launch point of the current atmospheric ionizing radiation (AIR) research project. Added emphasis to the need for reassessment of atmospheric radiation resulted from the major lowering of the recommended occupational exposure limits, the inclusion of aircrew as radiation workers, and the recognition of civil aircrew as a major source of occupational exposures. Furthermore, the work of Ferenc Hajnal of the Environmental Measurements Laboratory brought greater focus to the uncertainties in the neutron flux at high altitudes. A re-examination of the issues involved was committed at the Langley Research Center and by the National Council on Radiation Protection (NCRP). As a result of the NCRP review, a new flight package was assembled and flown during solar minimum at which time the galactic cosmic radiation is at a maximum (June 1997). The present workshop is the initial analysis of the new data from that flight. The present paper is an overview of the status of knowledge of atmospheric ionizing radiations. We will re-examine the exposures of the world population and examine the context of aircrew exposures with implications for the results of the present research. A condensed version of this report was given at the 1998 Annual Meeting of the NCRP with proceedings published in the journal of Health Physics.

Wilson, J. W.↗

NASA NTRS2004

Preliminary Analysis of X-Band and Ka-Band Radar for Use in the Detection of Icing Conditions Aloft

NASA and the U.S. Army Cold Regions Research and Engineering Laboratory (CRREL) have an on-going activity to develop remote sensing technologies for the detection and measurement of icing conditions aloft. Radar has been identified as a strong tool for this work. However, since the remote detection of icing conditions with the intent to identify areas of icing hazard is a new and evolving capability, there are no set requirements for radar sensitivity. This work is an initial attempt to quantify, through analysis, the sensitivity requirements for an icing remote sensing radar. The primary radar of interest for cloud measurements is Ka-band, however, since NASA is currently using an X-band unit, this frequency is also examined. Several aspects of radar signal analysis were examined. Cloud reflectivity was calculated for several forms of cloud using two different techniques. The Air Force Geophysical Laboratory (AFGL) cloud models, with different drop spectra represented by a modified gamma distribution, were utilized to examine several categories of cloud formation. Also a fundamental methods approach was used to allow manipulation of the cloud droplet size spectra. And an analytical icing radar simulator was developed to examine the complete radar system response to a configurable multi-layer cloud environment. Also discussed is the NASA vertical pointing X-band radar. The radar and its data system are described, and several summer weather events are reviewed.

Reehorst, Andrew L.↗

NASA NTRS2003

FAST/Polar Conjunction Study of Field-Aligned Auroral Acceleration and Corresponding Magnetotail Drivers

The discrete aurora results when energized electrons bombard the Earth's atmosphere at high latitudes. This paper examines the physical processes that can cause field-aligned acceleration of plasma particles in the auroral region. A data and theoretical study has been carried out to examine the acceleration mechanisms that operate in the auroral zone and to identi@ the magnetospheric drivers of these acceleration mechanisms. The observations used in the study were collected by the Fast Auroral Snapshot (FAST) and Polar satellites when the two satellites were in approximate magnetic conjunction in the auroral region. During these events FAST was in the middle of the auroral zone and Polar was above the auroral zone in the near-Earth plasma sheet. Polar data were used to determine the conditions in the magnetotail at the time field-aligned acceleration was measured by FAST in the auroral zone. For each of the magnetotail drivers identified in the data study, the physics of field-aligned acceleration in the auroral region was examined using existing theoretical efforts and/or a long-system particle in cell simulation to model the magnetically connected region between the two satellites. Results from the study indicate that there are three main drivers of auroral acceleration: (1) field-aligned currents that lead to quasistatic parallel potential drops (parallel electric fields), (2) earthward flow of high-energy plasma beams from the magnetotail into the auroral zone that lead to quasistatic parallel potential drops, and (3) large-amplitude Alfven waves that propagate into the auroral region from the magnetotail. The events examined thus far confm the previously established invariant latitudinal dependence of the drivers and show a strong dependence on magnetic activity. Alfven waves tend to occur primarily at the poleward edge of the auroral region during more magnetically active times and are correlated with intense electron precipitation. At lower latitudes away from the poleward edge of the auroral zone is the primary field-aligned current region which results in the classical field- aligned acceleration associated with the auroral zone (electrons earthward and ion beams tailward). During times of high magnetic activity, high-energy ion beams originating from the magnetotail are observed within, and overlapping, the regions of primary and return field-aligned current. Along the field lines where the high-energy magnetotail ion beams are located, field-aligned acceleration can occur in the auroral zone leading to precipitating electrons and upwelling ionospheric ion beams. Field-aligned currents are present during both quiet and active times, while the Alfven waves and magnetotail ion beams were observed only during more magnetically active events.

Schriver, D.↗

NASA NTRS2003

Shearography for Non-Destructive Evaluation with Applications to BAT Mask Tile Adhesive Bonding and Specular Surface Honeycomb Panels

In this report we examine the applicability of shearography techniques for nondestructive inspection and evaluation in two unique application areas. In the first application, shearography is used to evaluate the quality of adhesive bonds holding lead tiles to the BAT gamma ray mask for the NASA Swift program. By exciting the mask with a vibration, the more poorly bonded tiles can be distinguished by their greater displacement response, which is readily identifiable in the shearography image. A quantitative analysis is presented that compares the shearography results with a destructive pull test measuring the force at bond failure. Generally speaking, the results show good agreement. Further investigation would be useful to optimize certain test parameters such as vibration frequency and amplitude. The second application is to evaluate the bonding between the skin and core of a honeycomb structure with a specular (mirror-like) surface. In standard shearography techniques, the object under test must have a diffuse surface to generate the speckle patterns in laser light, which are then sheared. A novel configuration using the specular surface as a mirror to image speckles from a diffuser is presented, opening up the use of shearography to a new class of objects that could not have been examined with the traditional approach. This new technique readily identifies large scale bond failures in the panel, demonstrating the validity of this approach. For the particular panel examined here, some scaling issues should be examined further to resolve the measurement scale down to the very small size of the core cells. In addition, further development should be undertaken to determine the general applicability of the new approach and to establish a firm quantitative foundation.

Lysak, Daniel B.↗

NASA NTRS2004

Offline GCSS Intercomparison of Cloud-Radiation Interaction and Surface Fluxes

Simulations of deep tropical clouds by both cloud-resolving models (CRMs) and single-column models (SCMs) in the GEWEX Cloud System Study (GCSS) Working Group 4 (WG4; Precipitating Convective Cloud Systems), Case 2 (19-27 December 1992, TOGA-COARE IFA) have produced large differences in the mean heating and moistening rates (-1 to -5 K and -2 to 2 grams per kilogram respectively). Since the large-scale advective temperature and moisture "forcing" are prescribed for this case, a closer examination of two of the remaining external types of "forcing", namely radiative heating and air/sea hear and moisture transfer, are warranted. This paper examines the current radiation and surface flux of parameterizations used in the cloud models participating in the GCSS WG4, be executing the models "offline" for one time step (12 s) for a prescribed atmospheric state, then examining the surface and radiation fluxes from each model. The dynamic, thermodynamic, and microphysical fluids are provided by the GCE-derived model output for Case 2 during a period of very active deep convection (westerly wind burst). The surface and radiation fluxes produced from the models are then divided into prescribed convective, stratiform, and clear regions in order to examine the role that clouds play in the flux parameterizations. The results suggest that the differences between the models are attributed more to the surface flux parameterizations than the radiation schemes.

Tao, W.-K.↗

NASA NTRS2003

The impact of the new biology on radiation risks in space

Radiation is considered to be one of three or four major hazards for personnel in space and has emerged as the most critical issue to be resolved for long-term missions, both orbital and interplanetary. Space habitats are stressful and dangerous environments. Health and medical consequences arising from microgravity, stress, and trauma include weakened immune systems, increased viral activity, and loss of bone mass. The greatest risks from radiation are generally assumed to be cancers and possibly damage to the central nervous system. Synergistic effects arising from the other environmental hazards along with abscopal and exogenic factors are likely. Space programs represent an exceptional opportunity for examining the biological consequences of low-dose exposures of humans to radiation at every level of progression. Although astronauts are a relatively small population, they are healthy, physically active volunteers who undergo extensive testing and medical examinations before, during, and after protracted exposures with periodic follow-up examinations. The radiation environments along with other hazards are likewise monitored and documented. Extensive international research programs are in progress. Seven years ago the U.S. National Aeronautics and Space Administration established the National Space Biomedical Research Institute through a cooperative agreement with a consortium of research and academic institutions in order to address radiation issues through a concerted, programmatic effort. Advanced technologies are rapidly being incorporated into these programs to determine the significance of new biological data and to evaluate the interplay among the different medical hazards. Programmatic in vivo and in vitro studies of the processes leading to carcinogenesis are in progress. Drugs and dietary supplements are being examined at the cellular and in vivo levels to assess their potential as dose-modifying agents. The infrastructure of this new approach, recent results, and research in progress are reviewed and discussed.

NASA Discipline Radiation Health↗

NASA NTRS2003

Focused Assessment with Sonography for Trauma in weightlessness: a feasibility study

BACKGROUND: The Focused Assessment with Sonography for Trauma (FAST) examines for fluid in gravitationally dependent regions. There is no prior experience with this technique in weightlessness, such as on the International Space Station, where sonography is currently the only diagnostic imaging tool. STUDY DESIGN: A ground-based (1 g) porcine model for sonography was developed. We examined both the feasibility and the comparative performance of the FAST examination in parabolic flight. Sonographic detection and fluid behavior were evaluated in four animals during alternating weightlessness (0 g) and hypergravity (1.8 g) periods. During flight, boluses of fluid were incrementally introduced into the peritoneal cavity. Standardized sonographic windows were recorded. Postflight, the video recordings were divided into 169 20-second segments for subsequent interpretation by 12 blinded ultrasonography experts. Reviewers first decided whether a video segment was of sufficient diagnostic quality to analyze (determinate). Determinate segments were then analyzed as containing or not containing fluid. A probit regression model compared the probability of a positive fluid diagnosis to actual fluid levels (0 to 500 mL) under both 0-g and 1.8-g conditions. RESULTS: The in-flight sonographers found real-time scanning and interpretation technically similar to that of terrestrial conditions, as long as restraint was maintained. On blinded review, 80% of the recorded ultrasound segments were considered determinate. The best sensitivity for diagnosis in 0 g was found to be from the subhepatic space, with probability of a positive fluid diagnosis ranging from 9% (no fluid) to 51% (500 mL fluid). CONCLUSIONS: The FAST examination is technically feasible in weightlessness, and merits operational consideration for clinical contingencies in space.

NASA Discipline Life Sciences Technologies↗

NASA NTRS2001

Molecular microbial diversity of a spacecraft assembly facility

In ongoing investigations to map and archive the microbial footprints in various components of the spacecraft and its accessories, we have examined the microbial populations of the Jet Propulsion Laboratory's Spacecraft Assembly Facility (JPL-SAF). Witness plates made up of spacecraft materials, some painted with spacecraft qualified paints, were exposed for approximately 7 to 9 months at JPL-SAF and examined the particulate materials collected for the incidence of total cultivable aerobic heterotrophs and heat-tolerant (80 degrees C for 15-min.) spore-formers. The results showed that the witness plates coated with spacecraft qualified paints attracted more dust particles than the non-coated stainless steel witness plates. Among the four paints tested, witness plates coated with NS43G accumulated the highest number of particles, and hence attracted more cultivable microbes. The conventional microbiological examination revealed that the JPL-SAF harbors mainly Gram-positive microbes and mostly spore-forming Bacillus species. Most of the isolated microbes were heat resistant to 80 degrees C and proliferate at 60 degrees C. The phylogenetic relationships among 23 cultivable heat-tolerant microbes were examined using a battery of morphological, physiological, molecular and chemotaxonomic characterizations. By 16S rDNA sequence analysis, the isolates fell into seven clades: Bacillus licheniformis, B. pumilus, B. cereus, B. circulans, Staphylococcus capitis, Planococcus sp. and Micrococcus lylae. In contrast to the cultivable approach, direct DNA isolation, cloning and 16S rDNA sequencing analysis revealed equal representation of both Gram-positive and Gram-negative microorganisms.

Industry↗

NASA NTRS1998

Phylogenetic mapping of bacterial morphology

The availability of a meaningful molecular phylogeny for bacteria provides a context for examining the historical significance of various developments in bacterial evolution. Herein, the classical morphological descriptions of selected members of the domain Bacteria are mapped upon the genealogical ancestry deduced from comparison of small-subunit rRNA sequences. For the species examined in this study, a distinct pattern emerges which indicates that the coccus shape has arisen and accumulated independently multiple times in separate lineages and typically survived as a persistent end-state morphology. At least two other morphologies persist but have evolved only once. This study demonstrates that although bacterial morphology is not useful in defining bacterial phylogeny, it is remarkably consistent with that phylogeny once it is known. An examination of the experimental evidence available for morphogenesis as well as microbial fossil evidence corroborates these findings. It is proposed that the accumulation of persistent morphologies is a result of the biophysical properties of peptidoglycan and their genetic control, and that an evolved body-plan strategy based on peptidoglycan may have been a fate-sealing step in the evolution of Bacteria. More generally, this study illustrates that significant evolutionary insights can be obtained by examining biological and biochemical data in the context of a reliable phylogenetic structure.

NASA Discipline Exobiology↗

NASA NTRS2004

Kinetic Roughening and Energetics of Tetragonal Lysozyme Crystal Growth: A Preliminary Atomic Force Microscopy Investigation

We examined particulars of crystal growth from measurements obtained at both microscopic and molecular levels. The crystal growth measurements performed at the microscopic level are well characterized by a model that balances the flux of macromolecules towards the crystal surface with the flux of the crystal surface. Numerical evaluation of model with measurements of crystal growth, in time, provided accurate estimates for the average growth velocities. Growth velocities thus obtained were also interpreted using well-established phenomenological theories. Moreover, we find that microscopic measurements of growth velocity measurements obtained as a function of temperature best characterizes changes in crystal growth modes, when present. We also examined the possibility of detecting a change in crystal growth modes at the molecular level using atomic force microscopy, AFM. From preliminary AFM measurements performed at various supersaturations, we find that magnitude of surface height fluctuations, h(x), increases with supersaturation. Further examination of surface height fluctuations using methods established for fluctuation spectroscopy also enabled the discovery of the existence of a characteristic length, c, which may possibly determine the mode of crystal growth. Although the results are preliminary, we establish the non- critical divergence of 5 and the root-mean-square (rms) magnitude of height-height fluctuations as the kinetic roughening transition temperatures are approached. Moreover, we also examine approximate models for interpreting the non-critical behavior of both 6 and rms magnitude of height-height fluctuations, as the solution supersaturation is increased towards the kinetic roughening supersaturation.

Gorti, Sridhar↗

NASA NTRS2004

Goal-directed ultrasound in the detection of long-bone fractures

BACKGROUND: New portable ultrasound (US) systems are capable of detecting fractures in the remote setting. However, the accuracy of ultrasound by physicians with minimal ultrasound training is unknown. METHODS: After one hour of standardized training, physicians with minimal US experience clinically evaluated patients presenting with pain and trauma to the upper arm or leg. The investigators then performed a long-bone US evaluation, recording their impression of fracture presence or absence. Results of the examination were compared with routine plain or computer aided radiography (CT). RESULTS: 58 patients were examined. The sensitivity and specificity of US were 92.9% and 83.3%, and of the physical examination were 78.6% and 90.0%, respectively. US provided improved sensitivity with less specificity compared with physical examination in the detection of fractures in long bones. CONCLUSION: Ultrasound scans by minimally trained clinicians may be used to rule out a long-bone fracture in patients with a medium to low probability of fracture.

Validation Studies↗