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At least 775 records · Page 43

Environmental microbiome engineering for the mitigation of climate change

Abstract Environmental microbiome engineering is emerging as a potential avenue for climate change mitigation. In this process, microbial inocula are introduced to natural microbial communities to tune activities that regulate the long‐term stabilization of carbon in ecosystems. In this review, we outline the process of environmental engineering and synthesize key considerations about ecosystem functions to target, means of sourcing microorganisms, strategies for designing microbial inocula, methods to deliver inocula, and the factors that enable inocula to establish within a resident community and modify an ecosystem function target. Recent work, enabled by high‐throughput technologies and modeling approaches, indicate that microbial inocula designed from the top‐down, particularly through directed evolution, may generally have a higher chance of establishing within existing microbial communities than other historical approaches to microbiome engineering. We address outstanding questions about the determinants of inocula establishment and provide suggestions for further research about the possibilities and challenges of environmental microbiome engineering as a tool to combat climate change.

59 BASIC BIOLOGICAL SCIENCES↗

Influence of Correlations on the Thermal Performance Modeling of Parabolic Trough Collectors

The influence of correlations on the thermal performance modeling of parabolic trough collectors was analyzed in this work. A versatile model for a parabolic trough collector was developed that allows one- and two-dimensional analysis and enables the use of correlations to calculate thermophysical properties and convection heat transfer coefficients. The model also allows the use of constant values for properties and/or coefficients obtained from the evaluation correlations at a specific temperature. The effect of each correlation was evaluated independently, and the results were compared with a reference case that considered a two-dimensional approach and used all the correlations. For the analyzed cases, the correlation for the absorber emittance has the strongest impact on the collector efficiency, leading to a lower error when used. Based on the results, a one-dimensional model approach considering a correlation for the absorber emittance leads to efficiency errors below 3% for collector lengths of up to 243.6 m. Compared with the reference case, a one-dimensional approach using all correlations for a collector with a length of 500 m, and operating with an inlet temperature of 773 K, can result in errors around 9%. However, using constant values for properties and heat transfer coefficients could lead to errors of up to 50%. Multiple thermal models for parabolic trough collectors proposed in the literature rely on a one-dimensional approach, estimated values for the heat transfer coefficients, and constant thermophysical properties. The errors associated with those approaches are analyzed and quantified in this work as a function of the collector length and operation temperature.

absorber emittance↗

INSERVICE INSPECTION OF EXTENDED DRY STORAGE OF SPENT NUCLEAR FUEL, PART II: NDE/SENSOR TECHNOLOGY DEVELOPMENT AND CODIFICATION

This paper describes development and demonstration of nondestructive examination (NDE) technologies to support periodic examinations of interim dry cask storage system (DCSS) canisters for spent nuclear fuel in the USA to verify continued safe operation and that the canister confinement is intact and performing its intended safety function. Specifically, this work relates to NDE technology development for “canister” based DCSS systems which form the majority population of DCSSs, in the USA, for interim storage of spent nuclear fuel. Consideration of potential degradation of the welded stainless-steel canister in these systems is required for continued usage in the period of extended operation (PEO) beyond the initial license or certified term. Physical access to the canister surface is constrained due to narrow annulus spaces between the canister and the overpack, tortuous entry pathways, and high temperatures and radiation doses that can be damaging to materials and electronics related to inspections. Several activities to demonstrate NDE technologies for the inspections of different DCSS systems are summarized.

nondestructive examination (NDE), Dry Cask Storage↗

Elucidating the Role of Hydroxide Electrolyte on Anion-Exchange-Membrane Water Electrolyzer Performance

Many solid-state devices, especially those requiring anion conduction, often add a supporting electrolyte to enable efficient operation. The prototypical case is that of anion-exchange-membrane water electrolyzers (AEMWEs), where addition of an alkali metal solution improves performance. However, the specific mechanism of this performance improvement is currently unknown. This work investigates the functionality of the alkali metal solution in AEMWEs using experiments and mathematical models. The results show that additional hydroxide plays a key role not only in ohmic resistance of the membrane and catalyst layer but also in the reaction kinetics. The modeling suggests that the added liquid electrolyte creates an additional electrochemical interface with the electrocatalyst that provides ion-transport pathways and distributes product gas bubbles; the total effective electrochemical active surface area in the cell with 1 M KOH is 5 times higher than that of the cell with DI water. In the cell with 1 M KOH, more than 80% of the reaction current is associate with the liquid electrolyte. These results indicate the importance of high pH of electrolyte and catalyst/electrolyte interface in AEMWEs. The understanding of the functionality of the alkali metal solution presented in this study should help guide the design and optimization of AEMWEs.

25 ENERGY STORAGE↗

Deuteron-induced reactions on natural Zr from threshold to 50 MeV: production of 86g Y

Two stacks of thin Zr foils were irradiated with 30 and 50 MeV deuterons, respectively, using the Lawrence Berkeley National Laboratory 88-Inch Cyclotron, and 19 excitation functions for nat Zr(d,x) reactions were measured over a beam energy range of 6.3–47.64 MeV, where the independent cross sections for nat Zr(d,x) 88 Nb and nat Zr(d,x) 86m,g Y were measured for the first time. The well-characterized nat Fe(d,x) 56 Co, nat Ni(d,x) 56 Co, nat Ni(d,x) 58 Co, nat Ni(d,x) 61 Cu, nat Ti(d,x) 46 Sc and nat Ti(d,x) 48 V monitor reactions were used to determine the deuteron beam current throughout the stacks. All cross sections were determined using High Purity Germanium (HPGe) detector γ-ray spectroscopy. A variance minimization technique was employed to simultaneously constrain the deuteron beam currents with multiple monitor reactions, thus reducing systematic uncertainties. An additional 16 channels are reported for reactions on the nickel, titanium, and iron monitor foils, leading to a total of 35 excitation functions, with seven reaction channels reported for the first time in this work. The measured excitation functions are compared to calculations provided by the reaction modeling codes TALYS – 2.0, ALICE – 2020, CoH – 3.5.3 and EMPIRE – 3.2.3, as well as the TENDL – 2023 data library. The degree of agreement between theory and experiments is discussed. The possible production of the important PET radionuclide 86g Y via the nat Zr(d,x) route was critically examined. The physical yields for nat Zr(d,x) 86 Y and other yttrium isotopes produced were calculated and compared to other production pathways. Due to high-level of associated radionuclide impurities, this route cannot deliver 86g Y suitable for medical applications.

86gY↗

East River Pore Water FTICR-MS Data associated with "Geomorphic and Hydrogeochemical Controls on Fine-Scale Hyporheic Microbiome Assembly and Function"

Pore water samples were collected from distributed locations around Meander A in East River (Crested Butte, CO, USA) during the summer of 2018. This dataset consists of the characterization of dissolved organic matter using 12 Tesla Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS; in .xml format) analyzed through the Environmental Molecular Sciences Laboratory (EMSL; https://www.pnnl.gov/environmental-molecular-sciences-laboratory).This study aimed to understand organic matter assemblages differ within a streambed with differing geomorphology and the interplay between streambed organic matter and microbiome function. Any published work that utilizes data presented in this dataset should cite the dataset using the DOI number. If using the dataset, please acknowledge the U.S. Department of Energy (DOE) Office of Science (BER) Subsurface Biogeochemical Research (SBR) program, the Environmental Molecular Sciences Laboratory (EMSL), and Worldwide Hydrobiogeochemical Observation Network for Dynamic River Systems (WHONDRS). The publication DOI will be added to this abstract when available.

54 ENVIRONMENTAL SCIENCES↗

Mechanistic Source Term Considerations for Advanced Non-LWRs

This report is a functional review of the radionuclide containment strategies of fluoride-salt-cooled high temperature reactor (FHR), molten salt reactor (IVISR) and high temperature gas reactor (HTGR) systems. This analysis serves as a starting point for further, more in-depth analyses geared towards identifying phenomenological gaps that still exist, preventing the creation of a mechanistic source term for these reactor types. As background information to this review, an overview of how a mechanistic source term is created and used for consequence assessment necessary for licensing is provided. How mechanistic source term is used within the LMP is also provided. Third, the characteristics of non-LWR mechanistic source terms are examined This report does not assess the viability of any software system for use with advanced reactor designs, but instead covers system function requirements. Future work within the Nuclear Energy Advanced Modeling and Simulations (NEAMS) program will address such gaps.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Mechanistic Source Term Considerations for Advanced Non-LWRs (Revision 1)

This report is a functional review of the radionuclide containment strategies of fluoride-salt-cooled high temperature reactor (FHR), molten salt reactor (MSR) and high temperature gas reactor (HTGR) systems. This analysis serves as a starting point for further, more in-depth analyses geared towards identifying phenomenological gaps that still exist, hindering the creation of a mechanistic source term for these reactor types. As background information to this review, an overview of how a mechanistic source term is created and used for consequence assessment necessary for licensing is provided. How a mechanistic source term is used within the Licensing Modernization Project (LMP) is also provided. Lastly, the characteristics of non-LWR mechanistic source terms are examined. This report does not assess the viability of any software system for use with advanced reactor designs, but instead covers system function requirements. Future work within the Nuclear Energy Advanced Modeling and Simulations (NEAMS) program will address such gaps. This document is an update of SAND 2020-6730. An additional chapter is included as well as edits to original content.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Real-time Measurements of Complex Transition Metal Oxide Nanostructure Growth (Year 3 - Final Technical Report-GaTech-MIT)

The project, a collaboration between the Ross Lab at MIT and the Filler Lab at Georgia Tech, aimed to combine in situ microscopy and spectroscopy measurements to answer fundamental questions about the physics and chemistry governing the bottom-up vapor-solid-liquid (VLS) growth of one-dimensional (1-D) functional oxides. This work supported one PhD student and 1 postdoc. One paper has been published and 5 others are in preparation. To date, this work has been presented at 5 conferences.

36 MATERIALS SCIENCE↗

Analysis of Conservation Voltage Reduction under Inverter-Based VAR-Support [Slides]

Conservation voltage reduction (CVR) is a common technique used by utilities to strategically reduce demand during peak periods. As penetration levels of distributed generation (DG) continue to rise and advanced inverter capabilities become more common, it is unclear how the effectiveness of CVR will be impacted and how CVR interacts with advanced inverter functions. In this work, we investigated the mutual impacts of CVR and DG from photovoltaic (PV) systems (with and without autonomous Volt-VAR enabled). The analysis was conducted on an actual utility dataset, including a feeder model, measurement data from smart meters and intelligent reclosers, and metadata for more than 30 CVR events triggered by the utility over the year. The installed capacity of the modeled PV systems represented 66% of peak load, but reached instantaneous penetrations reached up to 2.5x the load consumption over the year. While the objectives of CVR and autonomous Volt-VAR are opposed to one another, this study found that their interactions were mostly inconsequential since the CVR events occurred when total PV output was low.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Infrared Photon Pair-Production in Ligand-Sensitized Lanthanide Nanocrystals

This report details spectroscopic characterizations of rare-earth, core-shell nanoparticles decorated with the f -element chelator 3,4,3-LI(1,2-HOPO). Evidence of photon downconversion is corroborated through detailed power dependence measurements, which suggest two-photon decay paths are active in these materials, albeit only representing a minority contribution of the sum luminescence, with emission being dominated by normal, Stokes' shifted fluorescence. Specifically, ultraviolet ligand photosensitization of Nd 3+ ions in a NaGdF 4 host shell results in energy transfer to a Nd 3+ /Yb 3+ -doped NaGdF 4 nanoparticle core. The population and subsequent decay of core, Yb 3+ 2 F 5/2 states result in a spectral shift of 620 nm, manifested in a NIR emission displaying luminescence profiles diagnostic of Yb 3+ and Nd 3+ excited state decays. Emphasis is placed on the generality of this material architecture for realizing ligand-pumped, multi-photon downconversion, with the Nd 3+ /Yb 3+ system presented here functioning as a working prototype for a design principle that may be readily extended to other lanthanide pairs.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Training Restricted Boltzmann Machines With a D-Wave Quantum Annealer

Restricted Boltzmann Machine (RBM) is an energy-based, undirected graphical model. It is commonly used for unsupervised and supervised machine learning. Typically, RBM is trained using contrastive divergence (CD). However, training with CD is slow and does not estimate the exact gradient of the log-likelihood cost function. In this work, the model expectation of gradient learning for RBM has been calculated using a quantum annealer (D-Wave 2000Q), where obtaining samples is faster than Markov chain Monte Carlo (MCMC) used in CD. Training and classification results of RBM trained using quantum annealing are compared with the CD-based method. The performance of the two approaches is compared with respect to the classification accuracies, image reconstruction, and log-likelihood results. The classification accuracy results indicate comparable performances of the two methods. Image reconstruction and log-likelihood results show improved performance of the CD-based method. It is shown that the samples obtained from quantum annealer can be used to train an RBM on a 64-bit “bars and stripes” dataset with classification performance similar to an RBM trained with CD. Though training based on CD showed improved learning performance, training using a quantum annealer could be useful as it eliminates computationally expensive MCMC steps of CD.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Targeting a cell surface vitamin D receptor on tumor-associated macrophages in triple-negative breast cancer

Triple-negative breast cancer (TNBC) is an aggressive tumor with limited treatment options and poor prognosis. We applied the in vivo phage display technology to isolate peptides homing to the immunosuppressive cellular microenvironment of TNBC as a strategy for non-malignant target discovery. We identified a cyclic peptide (CSSTRESAC) that specifically binds to a vitamin D receptor, protein disulfide-isomerase A3 (PDIA3) expressed on the cell surface of tumor-associated macrophages (TAM), and targets breast cancer in syngeneic TNBC, non-TNBC xenograft, and transgenic mouse models. Systemic administration of CSSTRESAC to TNBC-bearing mice shifted the cytokine profile toward an antitumor immune response and delayed tumor growth. Moreover, CSSTRESAC enabled ligand-directed theranostic delivery to tumors and a mathematical model confirmed our experimental findings. Finally, in silico analysis showed PDIA3-expressing TAM in TNBC patients. This work uncovers a functional interplay between a cell surface vitamin D receptor in TAM and antitumor immune response that could be therapeutically exploited.

59 BASIC BIOLOGICAL SCIENCES↗

Use of a torsional pendulum as a high-pressure gage and determination of viscosity of helium gas at high pressures

Three torsional crystal parameters were examined for suitability in sensing pressure in gases up to 131 million newtons per square meter. The best parameters were found to be the change in crystal decrement at resonance and the change in crystal electrical resistance at resonance. The change in crystal resonant frequency did not appear to be a reliable pressure measuring parameter. Pure argon and pure helium gases were studied for use as working fluids. Helium functioned better over a wider pressure range. Calibration of the gage also provided a measure of the viscosity-density product of the gas as a function of pressure. These data, together with known extrapolated density data, permitted the determination of the viscosity of helium to 131 million N/square meter.

Maisel, J. E.↗

Stirling cycle engine and refrigeration systems

A Stirling cycle heat engine is disclosed in which displacer motion is controlled as a function of the working fluid pressure P sub 1 and a substantially constant pressure P sub 0. The heat engine includes an auxiliary chamber at the constant pressure P sub 0. An end surface of a displacer piston is disposed in the auxiliary chamber. During the compression portion of the engine cycle when P sub 1 rises above P sub 0 the displacer forces the working fluid to pass from the cold chamber to the hot chamber of the engine. During the expansion portion of the engine cycle the heated working fluid in the hot chamber does work by pushing down on the engine's drive piston. As the working fluid pressure P sub 1 drops below P sub 0 the displacer forces most of the working fluid in the hot chamber to pass through the regenerator to the cold chamber. The engine is easily combinable with a refrigeration section to provide a refrigeration system in which the engine's single drive piston serves both the engine and the refrigeration section.

Higa, W. H.↗

The LEMMA concept - A new manipulator

The LEMMA is a proposed conceptual design of a remote teleoperator manipulator arm with 6 degrees of freedom, exclusive of the end-effector. The bevel gear arrangement in 4 of the 6 degrees of freedom, together with the concentric-tubular arm configuration, requires a unique and novel drive mechanism. This differential drive assembly, housed in the shoulder, is a self-compensating device and simplifies the matrix solution for the resolved motion rate control system needed to guide the arm in its performances. The article will describe the need for the 6 degrees of freedom as established by the work envelope, the functioning of the differential shoulder drive, the arm and joint configuration, and the anticipated degree of accuracy.

Kersten, L.↗

A study of the diffuse galactic gamma radiation

The observed diffuse galactic gamma radiation is compared to that predicted from galactic cosmic ray interactions with galactic matter and photons, assuming that on a broad scale the galactic cosmic rays in the plane are correlated with matter density. Recent considerations of the galactic diffuse matter distribution, particularly the molecular hydrogen, the galactic photon density, and a revised cosmic ray galactic scale height, are included. The predictions are compared to the observational gamma ray longitude distributions, the latitude distribution, and energy spectrum, including the COS-B satellite results, and the COS-B background estimate. Considering the uncertainties, the agreement between the theoretical predictions and the gamma ray data seems generally reasonable, suggesting that the general concepts are likely to be correct. Both the results determined here alone and in conjunction with other work calculating source functions assuming only cosmic ray matter contributions indicate no necessity for a significant point source contribution to the diffuse gamma radiation in the energy range being considered (E(gamma)10 MeV).

Fichtel, C. E.↗