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At least 775 records · Page 43

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DOE OSTI2020

Glycosidic linkage, N -acetyl side-chain, and other structural properties of methyl 2-acetamido-2-deoxy-β- D -glucopyranosyl-(1→4)-β- D -mannopyranoside monohydrate and related compounds

The crystal structure of methyl 2-acetamido-2-deoxy-β-D-glycopyranosyl-(1→4)-β-D-mannopyranoside monohydrate, C 15 H 27 NO 11 ·H 2 O, was determined and its structural properties compared to those in a set of mono- and disaccharides bearing N -acetyl side-chains in βGlcNAc aldohexopyranosyl rings. Valence bond angles and torsion angles in these side chains are relatively uniform, but C—N (amide) and C—O (carbonyl) bond lengths depend on the state of hydrogen bonding to the carbonyl O atom and N—H hydrogen. Relative to N -acetyl side chains devoid of hydrogen bonding, those in which the carbonyl O atom serves as a hydrogen-bond acceptor display elongated C—O and shortened C—N bonds. This behavior is reproduced by density functional theory (DFT) calculations, indicating that the relative contributions of amide resonance forms to experimental C—N and C—O bond lengths depend on the solvation state, leading to expectations that activation barriers to amide cis – trans isomerization will depend on the polarity of the environment. DFT calculations also revealed useful predictive information on the dependencies of inter-residue hydrogen bonding and some bond angles in or proximal to β-(1→4) O -glycosidic linkages on linkage torsion angles φ and ψ. Hypersurfaces correlating φ and ψ with the linkage C—O—C bond angle and total energy are sufficiently similar to render the former a proxy of the latter.

Chemistry↗

DOE OSTI2022

Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass

Abstract Background The metabolic engineering of high-biomass crops for lipid production in their vegetative biomass has recently been proposed as a strategy to elevate energy density and lipid yields for biodiesel production. Energycane and sugarcane are highly polyploid, interspecific hybrids between Saccharum officinarum and Saccharum spontaneum that differ in the amount of ancestral contribution to their genomes. This results in greater biomass yield and persistence in energycane, which makes it the preferred target crop for biofuel production. Results Here, we report on the hyperaccumulation of triacylglycerol (TAG) in energycane following the overexpression of the lipogenic factors Diacylglycerol acyltransferase 1-2 ( DGAT 1-2) and Oleosin 1 ( OLE 1) in combination with RNAi suppression of SUGAR-DEPENDENT 1 ( SDP 1) and Trigalactosyl diacylglycerol 1 ( TGD 1). TAG accumulated up to 1.52% of leaf dry weight (DW,) a rate that was 30-fold that of non-modified energycane, in addition to almost doubling the total fatty acid content in leaves to 4.42% of its DW. Pearson’s correlation analysis showed that the accumulation of TAG had the highest correlation with the expression level of ZmDGAT 1-2, followed by the level of RNAi suppression for SDP 1. Conclusions This is the first report on the metabolic engineering of energycane and demonstrates that this resilient, high-biomass crop is an excellent target for the further optimization of the production of lipids from vegetative tissues.

1-2↗

NASA NTRS1969

Self-consistent many-electron theory of electron work functions and surface potential characteristics for selected metals

Electron work functions, surface potentials, and electron number density distributions and electric fields in the surface region of 26 metals were calculated from first principles within the free electron model. Calculation proceeded from an expression of the total energy as a functional of the electron number density, including exchange and correlation energies, as well as a first inhomogeneity term. The self-consistent solution was obtained via a variational procedure. Surface barriers were due principally to many-body effects; dipole barriers were small only for some alkali metals, becoming quite large for the transition metals. Surface energies were inadequately described by this model, which neglects atomistic effects. Reasonable results were obtained for electron work functions and surface potential characteristics, maximum electron densities varying by a factor of over 60.

Smith, J. R.↗

NASA NTRS1973

Observations of disk-shaped bodies in free flight at terminal velocity

Ten disk-shaped models of a proposed nuclear heat source module were released from an aircraft and observed by radar. The initial launch attitude, spin rate, and mass of the models were varied. Significant differences were observed in the mode of flight and terminal velocity among models of different mass and launch attitudes. The data were analyzed to yield lift and drag coefficients as a function of Reynolds number. The total sea-level velocity of the models was found to be well correlated as a function of mass per unit frontal area. The demonstrated terminal velocity of the modular disk heat source, about 27 m/sec for this specific design, is only 33% of that of existing heat source designs.

Vorreiter, J. W.↗

NASA NTRS1974

Formulation of a distortion index based on peak compressor pressure ratios

In order to effectively use a compressor face total-pressure distortion index as a measure of inlet-engine compatibility, a correlation of distortion amplitude with stall margin must be developed with minimal scatter. A recent analysis of data recorded in extensive distortion screen tests with the J85-GE-13 turbojet engine has resulted in a correlation based on compressor discharge pressure ratioed to the minimum pressure at the compressor face. Simply by determining compressor stall lines with a single hub radial distortion pattern, a single tip radial pattern, and with undistorted inflow, the overall compressor pressure ratio at stall for even the most complex distortion pattern was found to be predictable.

Calogeras, J. E.↗

NASA NTRS1974

A comparison of interstellar Na I, Ca II, and K I absorption

The strongest and generally most useful optical interstellar absorption lines include the D lines of Na I, the H and K lines of Ca II, and the resonance doublet at 7600 A and 7664 A of K I. The interferometric data for these strongest atomic lines are examined in a comprehensive manner. The numerical results pertaining to the total column densities are collected in a table, which lists 82 stars. The correlations among some of the quantities listed in the table are displayed in a number of graphs.

Hobbs, L. M.↗

NASA NTRS1974

Formulation of a distortion index based on peak compressor pressure ratios

Calogeras, J. E.↗

NASA NTRS1975

Hot wire anemometry in transonic flow

The use of hot-wire anemometry for obtaining fluctuating data in transonic flows has been evaluated. From hot-wire heat loss correlations based on previous transonic data, the sensitivity coefficients for velocity, density, and total temperature fluctuations have been calculated for a wide range of test conditions and sensor parameters. For sensor Reynolds numbers greater than 20 and high sensor overheat ratios, the velocity sensitivity remains independent of Mach number and equal to the density sensitivity. These conclusions were verified by comparisons of predicted sensitivities with those from recent direct calibrations in transonic flows. Based on these results, techniques are presented to obtain meaningful measurements of fluctuating velocity, density, and Reynolds shear stress using hot-wire and hot-film anemometers. Examples of these measurements are presented for two transonic boundary layers.

Horstman, C. C.↗

NASA NTRS1975

An investigation of the waters in the lower Chesapeake Bay area

Since October of 1972 the Langley Research Center and the Old Dominion University have been engaged in a cooperative effort to investigate the waters of the lower Chesapeake Bay area. During the first year an intensive effort was made to collect water data at the time of Landsat-1 overpasses. Bands 5 and 6 of the multispectral scanner (MSS) were shown to be useful for monitoring total particles, although a daily calibration was required. Band 5 had a high correlation with sediment and under some conditions an internal correction for atmospheric interference was possible. The relation of sediment to particles was established by using the MSS radiance values, since the two parameters were not monitored at the same stations. A contouring program was developed to display the sediment variation in the lower Bay at the time of the Jan. 26, 1973, overpass. Attenuation coefficients monitored continuously by ship along three baselines were cross-correlated with radiance values on three days. Evidence for tidal effects was obtained by showing that correlations improved when ship data collected during the nearest 'tidal time' instead of physical time were used

Bowker, D. E.↗

NASA NTRS1977

Hot-wire anemometry in transonic flow

The use of hot-wire anemometry for obtaining fluctuating data in transonic flows has been evaluated. From hot-wire heat loss correlations based on previous transonic data, the sensitivity coefficients for velocity, density, and total temperature fluctuations have been calculated for a wide range of test conditions and sensor parameters. For sensor Reynolds number greater than 20 and high sensor overheat ratios, the velocity sensitivity remains independent of Mach number and equal to the density sensitivity. These conditions were verified by comparisons of predicted sensitivities with those from recent direct calibrations in transonic flows. Based on these results, techniques are presented to obtain meaningful measurements of fluctuating velocity, density, and Reynolds shear stress using hot-wire and hot-film anemometers. Example of these measurements are presented for two transonic boundary layers.

Horstman, C. C.↗

NASA NTRS1977

Observations of neutral composition and related ionospheric variations during a magnetic storm in February 1974

The neutral-atmosphere composition experiment on Atmosphere Explorer C measured N2, O, Ar, and He densities during a magnetic storm at altitudes down to about 160 km. At latitudes above 45 deg N, N2 and Ar densities generally increase during the storm, while He and O densities decrease. Below 45 deg N all densities tend to increase during the storm. The density increases at perigee indicate that density- or temperature-profile changes are taking place below 160 km. The return to prestorm conditions is very slow, demonstrating the integrating effect of the atmospheric response. A recent theoretical model incorporating thermospheric circulation and diffusion effects reproduces the longitudinally averaged data, including latitude trends and the asymmetry about the storm maximum. Comparison with the mass-spectrometer and incoherent-scatter empirical model shows qualitative agreement with latitude trends but not with storm asymmetry, while the earlier J71 model based on total mass density is not in agreement with observed latitudinal trends. A close correlation is found between in situ O/N2 measurements and in situ and ground-based ionosonde measurements of electron density.

Hedin, A. E.↗

NASA NTRS1980

The terminal Eocene event - Formation of a ring system around the earth

It is suggested that the formation of a ring system about the earth by particles and debris related to the North American strewn tektite field is responsible for the terminal Eocene event of 34 million years ago, in which severe climatic changes accompanied by widespread biological extinctions occurred. Botanical data is cited which implies a 20-C decrease in winter temperature with no change in summer temperature, and evidence of the correlation of the North American tektite fall, which is estimated to have a total mass of 10 to the 9th to 10 to the 10th tons, with the disappearance of five of the most abundant species of radiolaria is presented. The possible connection between the tektites and climatic change is argued to result from the screening of sunlight by an equatorial ring of trapped particles of extraterrestrial origin in geocentric orbit which would cut off sunlight only in the winter months. Such a ring, located at a distance of between 1.5 and 2.5 earth radii (the Roche limit) is estimated to have a lifetime of a few million years.

Okeefe, J. A.↗

NASA NTRS1983

Cloud observations with Nimbus-7 satellite data

A Nimbus-7 Cloud Data Processing Team was established in 1982 in order to implement cloud-related studies for as long as the spacecraft's Temperature Humidity IR radiometer and Total Ozone Mapping Spectrometer continue to operate. It will soon be possible to correlate the Nimbus-7 cloud cover information with International Satellite Cloud Climatology results. The production of validated Nimbus-7 cloud products was scheduled to begin in November, 1983; each year of Nimbus-7 cloud data should take about four months to produce.

Stowe, L. L.↗

NASA NTRS1988

Satellite remote sensing and ozonesonde observation of ozone vertical profile and severe storm development

Two year ozonesonde data, January 1981 to December 1982, observed at four Canadian stations, and two-and-a-half year backscattered ultraviolet experiment data on the Nimbus-4 satellite, April 1970 to August 1972, observed over five U.S. stations, were used to study the relationship between the total ozone, vertical distribution of the ozone mixing ratio, height of half the total ozone, and the variation of local tropopause height. In view of the correlation between the variation of the tropopause height and the possible development of severe storms, a better understanding of the effect of the vertical distribution of the local ozone profile on the variation of the tropopause height can give considerable insight into the development of severe storms.

Hung, R. J.↗

NASA NTRS1990

Evidence against a simple two-component model for the far-infrared emission from galaxies

Two of the first Infrared Astronomy Satellite (IRAS) results were that galaxies have a wide range of values for the ratio of 60 micron to 100 micron flux density (0.2 less than or equal to S sub 60/S sub 100 less than or equal to 1.0) and that this ratio is correlated with L sub fir, L sub b, L sub fir being the total far-infrared luminosity and L sub b being the luminosity at visible wavelengths (de Jong et al. 1984; Soifer et al. 1984). From these results arose the following simple model for the far-infrared emission from galaxies (de Jong et al. 1984), which has remained the standard model ever since. In this model, the far-infrared emission comes from two dust components: warm dust (T approx. equals 50 K) intermingled with, and heated by, young massive OB stars in molecular clouds and HII regions, and colder dust (T approx. equals 20 K) associated with the diffuse atomic hydrogen in the interstellar medium and heated by the general interstellar radiation field. As the number of young stars in a galaxy increases, S sub 60/S sub 100 increases, because there is a greater proportion of warm dust, and so does L sub fir/L sub b, because most of the radiation from the young stars is absorbed by the dust, leading to a swifter increase in far-infrared emission than in visible light. Although this model explains the basic IRAS results, it is inelegant. It uses two free parameters to fit two data (the 60 and 100 micron flux densities)-and there are now several observations that contradict it. Despite these major problems with the two-component model, it is not clear what should be put in its place. When considering possible models for the far-infrared emission from galaxies, the observational evidence for our own galaxy must be considered. Researchers suspect that the study by Boulanger and Perault (1988) of the far-infrared properties of the local interstellar medium may be particularly relevant. They showed that molecular clouds are leaky - that most of the light from OB stars in molecular clouds does not heat the dust in the clouds, but instead leaks out. The consequence of this is that that while most of the far-infrared emission from the solar neighborhood is from dust associated with diffuse HI, this dust is mostly heated by young stars.

Eales, Stephen A.↗

NASA NTRS1956

Effect of an Adjustable Supersonic Inlet on the Performance up to Mach Number 2.0 of a J34 Turbojet Engine

A J34 turbojet engine was investigated at free-stream Mach numbers of 0.12 and 1.6 to 2.0 to determine the effect of supersonic inlet operation on engine performance. With the excepetion of ideal jet thrust, the use of generalized engine parameters correlated the engine data satisfactorily when the exit nozzle was choked. Large total-pressure distortations did not affect compressor efficiency. During inlet pulsing, the engine operated with compressor-inlet total-pressure variations as large as 18 percent of the local average and at frequnecies of 17 plus or minus 2 cycles per second. Total-pressure amplitudes propagated through the engine and caused ideal-gross-thrust changes up to 12 percent.

Beke, Andrew↗

NASA NTRS2001

A Diagnostic Analysis of the Kennedy Space Center LDAR Network 2. Cross-Sensor Studies

Range dependencies in total (intracloud and cloud to ground) lightning observed by the Kennedy Space Center Lightning Detection and Ranging (LDAR) network are established through cross comparison with other lightning sensors. Using total lightning observed from space by the Lightning Imaging Sensor (LIS), MAR flash detection efficiency is shown to remain above 90% out to 90-100 km range, and to be below 25% at 200 km range. MAR VHF source location error distributions are also determined as a function of range and are found to be asymmetric with standard deviation increasing roughly as r 2 . Range normalization schemes for total VHF source density are tested and shown to yield significant improvements in correlation with National Lightning Detection Network (NLDN) ground flash density at hourly, daily, monthly, and climatological timescales (up to 50% over uncorrected source densities using an exponential-in-range correction factor with 40-50 km e-folding scale).

Boccippio, D. J.↗

NASA NTRS2003

Proton Nonionizing Energy Loss (NIEL) for Device Applications

Nonionizing energy loss (NIEL) is a quantity that describes the rate of energy loss due to atomic displacements as a particle traverses a material. The product of the NIEL and the particle fluence (time integrated flux) gives the displacement damage energy deposition per unit mass of material. NIEL plays the same role to the displacement damage energy deposition as the stopping power to the total ionizing dose (TID). The concept of NIEL has been very useful for correlating particle induced displacement damage effects in semiconductor and optical devices. Many studies have successfully demonstrated that the degradation of semiconductor devices or optical sensors in a radiation field can be linearly correlated to the displacement damage energy, and subsequently to the NIEL deposited in the semiconductor devices or optical sensors. In addition, the NIEL concept was also useful in the study of both Si and GaAs solar cells and of high temperature superconductors, and at predicting the survivability of detectors used at the LHC at CERN. On the other hand, there are some instances where discrepancies are observed in the application of NIEL, most notably in GaAs semiconductor devices. However, NIEL is still a valuable tool, and can be used to scale damages produced by different particles and in different environments, even though this is not understood at the microscopic level.

Jun, Insoo↗