Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “target tracking”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 775 records · Page 43
All sourcesNASA NTRSDOE OSTI
NASA NTRS2009

Integration of Airborne Aerosol Prediction Systems and Vegetation Phenology to Track Pollen for Asthma Alerts in Public Health Decision Support Systems

The residual signal indicates that the pollen event may influence the seasonal signal to an extent that would allow detection, given accurate QA filtering and BRDF corrections. MODIS daily reflectances increased during the pollen season. The DREAM model (PREAM) was successfully modified for use with pollen and may provide 24-36 hour running pollen forecasts. Publicly available pollen forecasts are linked to general weather patterns and roughly-known species phenologies. These are too coarse for timely health interventions. PREAM addresses this key data gap so that targeting intervention measures can be determined temporally and geospatially. The New Mexico Department of Health (NMDOH) as part of its Environmental Public Health Tracking Network (EPHTN) would use PREAM a tool for alerting the public in advance of pollen bursts to intervene and reduce the health impact on asthma populations at risk.

Luvall, Jeffrey C.↗
DOE OSTI2024

Priming antibody responses to the fusion peptide in rhesus macaques

Immunodominance of antibodies targeting non-neutralizing epitopes and the high level of somatic hypermutation within germinal centers (GCs) required for most HIV broadly neutralizing antibodies (bnAbs) are major impediments to the development of an effective HIV vaccine. Rational protein vaccine design and non-conventional immunization strategies are potential avenues to overcome these hurdles. Here, we report using implantable osmotic pumps to continuously deliver a series of epitope-targeted immunogens to rhesus macaques over the course of six months to prime and elicit antibody responses against the conserved fusion peptide (FP). GC responses and antibody specificities were tracked longitudinally using lymph node fine-needle aspirates and electron microscopy polyclonal epitope mapping (EMPEM), respectively, to show antibody responses to the FP/N611 glycan hole region were primed, although exhibited limited neutralization breadth. Application of cryoEMPEM delineated key residues for on-target and off-target responses that can drive the next round of structure-based vaccine design.

60 APPLIED LIFE SCIENCES↗
NASA NTRS2021

Polarimetric calibration of the Multi-Angle Imager for Aerosols (MAIA)

NASA’s Multi-Angle Imager for Aerosols (MAIA) mission, under development at the Jet Propulsion Laboratory, is designed to study the adverse health effects of different types of particulate air pollution. Planned for launch in 2023 for a 3-year mission, the MAIA satellite instrument will focus on a selected set of metropolitan target areas, where air quality monitors and health data are available. Aerosol concentration and speciation are inferred from multi-angle measurements of backscattered sunlight in 14 spectral bands from 350-2200 nm, with bands near 442, 645 and 1040 nm measuring the degree (DoLP) and angle of linear polarization (AoLP) in addition to radiance. The pushbroom camera has a ~240-km cross-track field of view with a nadir resolution of ~200 m, and is mounted onto a biaxial gimbal to provide along-track view angles within ±60°, to extend the field of regard to ±48°, and to view the instrument’s onboard calibrator (OBC) and dark target. The OBC consists of a sunlit transmissive diffuser, followed by 12 polarizers at different orientations. MAIA’s polarimetry is implemented using miniature wire grid polarizers on the focal plane array, and dual photoelastic modulators (PEMs) and achromatic quarter-wave plates to rapidly rotate the polarization. The resulting ~26-Hz intensity modulation encodes the linearly polarized and total radiance in each pixel, leaving the DoLP and AoLP insensitive to gain calibration. We report on the polarimetric calibration of the MAIA camera using a vacuum-compatible polarization state generator, consisting of a 1600W Xenon lamp, 12-inch integrating sphere, and rotating high-extinction polarizer. Mueller-matrix-based calibration coefficients for each detector pixel are derived from measurements at multiple polarizer angles, and are used to correct the measurements for instrumental polarization aberrations. Prior to flight, the calibrated MAIA camera is panned across the OBC to characterize its output, using uniform illumination with an irradiance similar to the Sun.

Werne, Thomas↗
NASA NTRS1972

A Monte Carlo error analysis program for near-Mars, finite-burn, orbital transfer maneuvers

A computer program was developed which performs an error analysis of a minimum-fuel, finite-thrust, transfer maneuver between two Keplerian orbits in the vicinity of Mars. The method of analysis is the Monte Carlo approach where each off-nominal initial orbit is targeted to the desired final orbit. The errors in the initial orbit are described by two covariance matrices of state deviations and tracking errors. The function of the program is to relate these errors to the resulting errors in the final orbit. The equations of motion for the transfer trajectory are those of a spacecraft maneuvering with constant thrust and mass-flow rate in the neighborhood of a single body. The thrust vector is allowed to rotate in a plane with a constant pitch rate. The transfer trajectory is characterized by six control parameters and the final orbit is defined, or partially defined, by the desired target parameters. The program is applicable to the deboost maneuver (hyperbola to ellipse), orbital trim maneuver (ellipse to ellipse), fly-by maneuver (hyperbola to hyperbola), escape maneuvers (ellipse to hyperbola), and deorbit maneuver.

Green, R. N.↗
NASA NTRS2004

Passive IFF: Autonomous Nonintrusive Rapid Identification of Friendly Assets

A proposed optoelectronic instrument would identify targets rapidly, without need to radiate an interrogating signal, apply identifying marks to the targets, or equip the targets with transponders. The instrument was conceived as an identification, friend or foe (IFF) system in a battlefield setting, where it would be part of a targeting system for weapons, by providing rapid identification for aimed weapons to help in deciding whether and when to trigger them. The instrument could also be adapted to law-enforcement and industrial applications in which it is necessary to rapidly identify objects in view. The instrument would comprise mainly an optical correlator and a neural processor (see figure). The inherent parallel-processing speed and capability of the optical correlator would be exploited to obtain rapid identification of a set of probable targets within a scene of interest and to define regions within the scene for the neural processor to analyze. The neural processor would then concentrate on each region selected by the optical correlator in an effort to identify the target. Depending on whether or not a target was recognized by comparison of its image data with data in an internal database on which the neural processor was trained, the processor would generate an identifying signal (typically, friend or foe ). The time taken for this identification process would be less than the time needed by a human or robotic gunner to acquire a view of, and aim at, a target. An optical correlator that has been under development for several years and that has been demonstrated to be capable of tracking a cruise missile might be considered a prototype of the optical correlator in the proposed IFF instrument. This optical correlator features a 512-by-512-pixel input image frame and operates at an input frame rate of 60 Hz. It includes a spatial light modulator (SLM) for video-to-optical image conversion, a pair of precise lenses to effect Fourier transforms, a filter SLM for digital-to-optical correlation-filter data conversion, and a charge-coupled device (CCD) for detection of correlation peaks. In operation, the input scene grabbed by a video sensor is streamed into the input SLM. Precomputed correlation-filter data files representative of known targets are then downloaded and sequenced into the filter SLM at a rate of 1,000 Hz. When there occurs a match between the input target data and one of the known-target data files, the CCD detects a correlation peak at the location of the target. Distortion- invariant correlation filters from a bank of such filters are then sequenced through the optical correlator for each input frame. The net result is the rapid preliminary recognition of one or a few targets.

Moynihan, Philip↗
DOE OSTI2020

In situ monitoring of the stopped muon flux at Mu2e

The specific object of Mu2e search is the neutrino-less coherent $\mu^-\rightarrow e^-$ conversion in the field of an aluminum nucleus and the signal is a monoenergetic electron of energy $ \approx 104.97$ MeV. This process is forbidden in the Standard Model but allowed in many of its extensions: with minimal changes to include neutrino masses and oscillation, the Branching Ratio of this process, or the similarly interesting $\mu\rightarrow e \gamma$ decay, is expected to be of the order of $\mathcal{O}(10^{-54})$. Values like these are below any currently achievable experimental sensitivity, and the observation of a signal would be unambiguous evidence of New Physics. The upper limit on the muon conversion was set by SINDRUM II at $7\times10^{-13}$ (90\% C.L.) and the goal of the Mu2e collaboration is an improvement of 4 order of magnitudes.\\ The Mu2e experiment can be conceptually divided into three stages: interaction of the primary proton pulse with the tungsten target a nd production of $\pi$ and $\mu$; collection and transport of the produced particles down to the aluminum stopping target; interaction with the stopping target and measurement of the output particles. The measurement of the stopped muon flux is of cardinal importance for the normalization of the Mu2e results. The baseline design includes two detectors developed to measure this flux, counting $\gamma$ emitted by the muonic atoms: HPGe and LaBr$_3$(Ce). The studies performed by the Mu2e Collaboration show that these two systems can be reliably used to determine the overall normalization.\\ The number of stopped muons is proportional to the number of protons on target, which itself depends on the extraction system for the proton beam. The Mu2e beam delivery system will use a resonant extraction to create the proton pulses and this method is characterized by intensity fluctuations on the time scale of milliseconds. These fluctuations, aside from the proportional fluctuation of t he number of muons, have a non-trivial impact on the overall! performance of the apparatus. For example, higher intensity proton bunches translate into higher veto rates by the Cosmic Rays Veto system, which is triggered by secondary particles generated by the proton interaction. Another example is the effect of the instantaneous luminosity on the reconstruction efficiency: higher intensity translates into higher detector occupancy, which in turn reduces the reconstruction efficiency.\\ The two cited detectors encounter limitations when trying to monitor the flux at the millisecond timescales: HPGe is by construction a slow detector while the rate of emission of the $\gamma (1809\textrm{ keV})$ the LaBr$_3$(Ce) will measure is too low. As of today, no system allows to monitor these fluctuations and the goal of this Thesis is to try filling this gap. The method we developed to monitor the fluctuations of the stopped muon flux, and by extension, the fluctuation in the proton pulses intensity is based on counting the number of muons capt ured in the stopping target by counting the number of protons produced in the process of the nuclear muon capture. This is one of the possible processes a stopped muon can undergo and it can lead to the ejection of charged particles.\\ The reconstruction algorithms were developed by the Mu2e collaboration with the explicit (and sometimes implicit) aim to reconstruct tracks of electrons in an energy range of a conversion electron. The protons we are interested in behave quite differently from these electrons and the cardinal task of this study has been to tailor the reconstruction routines to these particles. This has been done by analyzing simulated events consisting of a single proton to optimize the procedure and understand the features of these tracks. A major difference with respect to the electrons is the non-relativistic nature of $100\div300$ MeV$/c$ protons: accounting for the low velocity is both a challenge and a unique signature of the tracks.\\ The use of a pulse d beam and the need to allow produced $\pi$ to decay and $\m! u$ to int! eract in the stopping target forces the definition of a Mu2e \textit{event}: an event is comprised by everything which happens in a $1.7\ \mu$s window between the incidence two consecutive proton pulses. This means that the reconstruction will be performed in an environment crowded by many different particles. The next step of this study has been to estimate the performance of the method in a fully-fledged Mu2e simulated event and to study what kind of selections would improve it.\\ Our results are satisfactory since the number of reconstructed protons is significant (few per event), and show that a monitor on the timescale of milliseconds is indeed possible.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2024

Full Simulation of CMS for Run-3 and Phase-2

In this contribution we report the status of the CMS Geant4 simulation and the prospects for Run-3 and Phase-2. Firstly, we report about our experience during the start of Run-3 with Geant4 10.7.2, the common software package DD4hep for geometry description, and VecGeom runtime geometry library. In addition, FTFP_BERT_EMM Physics List and CMS configuration for tracking in magnetic field have been utilized. For the first time, for the Grid mass production of Monte-Carlo, this combination of components is used. Further simulation improvements are under development targeting Run-3 such as the switch to the new Geant4 11.1 in production, that provides several features important for the optimization of simulation, for example the new transportation process with built-in multiple scattering, neutron general process, custom tracking manager, G4HepEm sub-library, and others. We will present evaluation of various options, validation results, and the final choice of simulation configuration for 2023 production and beyond. The performance of the CMS full simulation for Run-2 and Run-3 will also be discussed. CMS development plan for the Phase-2 Geant4 based simulation is very ambitious, and it includes a new geometry description, physics, and simulation configurations. The progress on new detector descriptions and full simulation will be presented as well as the R&D in progress to reduce compute capacity needs.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
NASA NTRS2009

Navigating the Return Trip from the Moon Using Earth-Based Ground Tracking and GPS

NASA s Constellation Program is planning a human return to the Moon late in the next decade. From a navigation perspective, one of the most critical phases of a lunar mission is the series of burns performed to leave lunar orbit, insert onto a trans-Earth trajectory, and target a precise re-entry corridor in the Earth s atmosphere. A study was conducted to examine sensitivity of the navigation performance during this phase of the mission to the type and availability of tracking data from Earth-based ground stations, and the sensitivity to key error sources. This study also investigated whether GPS measurements could be used to augment Earth-based tracking data, and how far from the Earth GPS measurements would be useful. The ability to track and utilize weak GPS signals transmitted across the limb of the Earth is highly dependent on the configuration and sensitivity of the GPS receiver being used. For this study three GPS configurations were considered: a "standard" GPS receiver with zero dB antenna gain, a "weak signal" GPS receiver with zero dB antenna gain, and a "weak signal" GPS receiver with an Earth-pointing direction antenna (providing 10 dB additional gain). The analysis indicates that with proper selection and configuration of the GPS receiver on the Orion spacecraft, GPS can potentially improve navigation performance during the critical final phases of flight prior to Earth atmospheric entry interface, and may reduce reliance on two-way range tracking from Earth-based ground stations.

Berry, Kevin↗
DOE OSTI2026

Filling the gap: hunting for vector bosons at the MUonE experiment with displaced decay signature

The upcoming MUonE experiment aims to precisely measure the running of the fine structure constant via elastic muon-electron scattering, to shed light on the current tension in the muon’s anomalous magnetic moment. In addition to its primary function as a precision experiment, MUonE also offers a unique testing ground to probe long-lived vector bosons. Such vector bosons can be produced via μe → μeV or μN → μNV scattering and decay into an electron/positron pair a few centimeters away from the interaction point. With its high-resolution tracking system and unique geometric design, MUonE is well-suited to reconstruct displaced vertices close to the target, allowing it to probe parameter space previously unattainable at colliders and longer-baseline beam dump experiments. We present a comprehensive study of the discovery potential of BSM vector boson mediators at the MUonE experiment. We show that MUonE can fill the long-standing gap in the parameter space of vector boson mediators with masses up to around 100 MeV.

Models for Dark Matter↗
DOE OSTI2025

Absolute Hugoniot measurements in low-density plastic foams on the NIKE shock compression platform

The propagation of Mbar-range shock waves in low-density foam materials is of interest to target design in all approaches to inertial confinement fusion, high energy density physics, and laboratory astrophysics. We report absolute Hugoniot measurements for CH 1.72 N 0.086 O 0.37 plastic foams with 73 and 94 mg/cm 3 densities in the 32–107 km/s shock velocity range. The experiments were performed on the shock compression platform developed on the NIKE KrF laser facility at the U.S. Naval Research Laboratory. NIKE's 4 or 8-ns long flat-top laser pulses drive steady shock waves into foam targets at the ablative pressures of 1–7 Mbar. The propagation of the ablation and shock fronts is tracked continuously in time using streaked side-on monochromatic x-ray imaging radiography. The straight x−t trajectories of the shock and ablation fronts in the recorded streak images confirmed their steadiness. The SESAME tabulated equation of state predictions generally agrees with our Hugoniot data within a 95% confidence band. The experimental uncertainty of the evaluated shock density compression ratios remains large, indicating the need for more experiments and improved theoretical understanding of the strong shock propagation mechanisms in dry foams.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2025

Mapping the cosmic gamma-ray horizon: the 1CGH catalogue of Fermi -LAT detections above 10 GeV

ABSTRACT We present the First Cosmic Gamma-ray Horizon (1CGH) catalogue, featuring $\gamma$-ray detections above 10 GeV based on 16 yr of observations with the Fermi Large Area Telescope (Fermi-LAT) satellite. After carefully selecting a sample of blazars and blazar candidates from catalogues in the literature, we performed a binned likelihood analysis and identified 2791 $\gamma$-ray emitters above 10 GeV, at >3$\sigma$ level, including 62 that are new $\gamma$-ray detections. For each source, we estimated the mean energy of the highest energy bin and analysed them in the context of the cosmic gamma-ray horizon. By adopting a reference model for the extragalactic background light (EBL), we identified a subsample of 525 sources where moderate to severe $\gamma$-ray absorption could be detected across the redshift range of 0–3. This work provides the most up-to-date compilation of detections above 10 GeV, along with their redshift information. We condense extensive results from the literature, including reports on observational campaigns dedicated to blazars and $\gamma$-ray sources, thereby delivering an unprecedented review of the redshift information for sources detected above 10 GeV. Additionally, we highlight key 1CGH sources where redshift information remains incomplete, offering guidance for future optical observation campaigns. The 1CGH catalogue aims to track the most significant sources for studying the $\gamma$-ray transparency of the Universe. Furthermore, it provides a targeted subsample where the EBL optical depth, $\tau _{(E,z)}$, can be robustly measured using Fermi-LAT data.

Arsioli, Bruno (ORCID:0000000181666602)↗
DOE OSTI2022

Superconvergence of Online Optimization for Model Predictive Control

We develop a one-Newton-step-per-horizon, online, lag-L, model predictive control (MPC) algorithm for solving discrete-time, equality-constrained, nonlinear dynamic programs. Based on recent sensitivity analysis results for the target problems class, we prove that the approach exhibits a behavior that we call superconvergence; that is, the tracking error with respect to the full horizon solution is not only stable for successive horizon shifts, but also decreases with increasing shift order to a minimum value that decays exponentially in the length of the receding horizon. The key analytical step is the decomposition of the one-step error recursion of our algorithm into algorithmic error and perturbation error. We show that the perturbation error decays exponentially with the lag between two consecutive receding horizons, while the algorithmic error, determined by Newton’s method, achieves quadratic convergence instead. Overall this approach induces our local exponential convergence result in terms of the receding horizon length for suitable values of L. In conclusion, numerical experiments validate our theoretical findings.

97 MATHEMATICS AND COMPUTING↗
DOE OSTI2023

A Planetary Defense System For Responsive Space Missions

As King et al succinctly write, “Collision between the Earth and small objects in the solar system are known to occur regularly, and can result in extinction-level events at their most energetic or more commonly widespread loss of life or economic destruction even for relatively low-energy impacts.” Currently, the JPL Sentry System and the NASA NEO Surveyor mission track, identify, and monitor potential asteroid threats. The JPL Sentry System4 is an automated collision monitoring system which scans the current asteroid catalog for potential impact trajectories with Earth. A 2017 study led by P. Tricarico titled “The near-Earth asteroid population from two decades of observations” analyzes the combined observations of nine leading asteroid surveys. The NASA NEO Surveyor mission seeks to identify 90% of near-Earth objects (NEO) with diameters greater than 140 meters (460 feet) within 10 years. The NEO Surveyor combines data from two infrared imaging detectors to measure NEO size, composition, shape, rotational state, and orbits. Recently, the NASA Double Asteroid Redirection Test (DART) mission successfully altered the orbit of asteroid moon Dimorphos in 2022. This demonstrated that orbit alteration is possible. Thus, collisions between the Earth and asteroids are both likely and demonstrate potential for great harm. There is ongoing work to identify, catalog, and track potential threats. However, when a threat is identified there is not yet a system in place to target the threatening asteroid and defend the planet from potentially existential threats. Building on the DART Mission, we explore a planetary defense system to mitigate asteroid threats.

42 ENGINEERING↗
NASA NTRS1989

Bandwidth-limited robust nonlinear sliding control of pointing and tracking maneuvers

It is shown how multiaxial spacecraft tracking and pointing maneuvers with known control bandwidth and given tracking error bounds can be implemented by variable-structure control in the presence of uncertain vehicle and target dynamics. It is shown how to select a nonlinear sliding surface relating attitude and rate variables, as well as a Lyapunov function in the surface variables that absorbs multiplicative model undertainties, thereby simplifying the computation of control corrections. It is then shown how a boundary layer envelope can be designed, within which the components of the surface error dynamics can be modeled as the outputs of designer-selected decoupled low-pass filters. Closed-loop stability conditions accounting for the coupling between the attitude error dynamics and the surface error dynamics are then obtained.

Dwyer, Thomas A. W., III↗
NASA NTRS2008

Stardust Entry Reconstruction

An overview of the reconstruction analyses performed for the Stardust capsule entry is described. The results indicate that the actual entry was very close to the pre-entry predictions. The capsule landed 8.1 km north-northwest of the desired target at Utah Test and Training Range. Analyses of infrared video footage and radar range data (obtained from tracking stations) during the descent show that drogue parachute deployment was 4.8 s later than the pre-entry prediction, while main parachute deployment was 19.3 s earlier than the pre-set timer indicating that main deployment was actually triggered by the backup baroswitch. Reconstruction of a best estimated trajectory revealed that the aerodynamic drag experienced by the capsule during hypersonic flight was within 1% of pre-entry predications. Observations of the heatshield support the pre-entry estimates of small hypersonic angles of attack, since there was very little, if any, charring of the shoulder region or the aftbody. Through this investigation, an overall assertion can be made that all the data gathered from the Stardust capsule entry were consistent with flight performance close to nominal pre-entry predictions. Consequently, the design principles and methodologies utilized for the flight dynamics, aerodynamics, and aerothermodynamics analyses have been corroborated.

Desai, Prasun N.↗
NASA NTRS2020

Pterodactyl: Non-Propulsive Control System Designs for Future Planetary Missions

Advances in deployable entry vehicle (DEV) technology, entry guidance, woven thermal protection systems, and affordable launch services make it possible to conceive of entry vehicles that optimize maneuverability, usable payload mass and volume, and operational costs. NASA's Space Technology Mission Directorate is currently funding the authors on a project, Pterodactyl, that is using on-the-fly trajectory design and integrated software and hardware development to investigate non-propulsive entry control systems for precision targeting of mechanical DEVs. The authors recently reported developments of these control systems for an asymmetric DEV to track bank commands for a lunar return entry. For this presentation, the authors will highlight key findings from their studies and propose rapid investigations of applications to future Mars missions such as sample return and asset delivery. Pterodactyl entry vehicle designs are suited to handle sensitive payloads and poised to achieve greater payload mass and volume compared to heritage entry vehicles given a particular launch vehicle. Furthermore, these designs could be adapted to launch on less costly launch vehicles as secondary payloads and could enable missions with high-frequency deployment requirements.

Alunni, Antonella↗
NASA NTRS2020

Efficient Starshade Retargeting Architecture Using Chemical Propulsion

NASA is studying a possible starshade flying in formation with the Nancy Grace Roman Space Telescope (Roman). The starshade would perform weeks-long translational retarget- ing maneuvers between target stars. A retargeting architecture is introduced that is based on chemical propulsion and does not require ground tracking or interactions with the telescope during the retargeting cruise. Feasibility is demonstrated through a verified covariance analysis of the starshade-telescope relative position over several weeks using realistic sensor and actuator assumptions. Performance is sufficient for Roman to reacquire the starshade after retargeting and the architecture is shown to also be applicable to other mission concepts such as the Habitable Exoplanet Observatory (HabEx).

Lisman, P. Douglas↗
DOE OSTI2024

Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus‐mediated sgRNA delivery

SUMMARY The requirement of in vitro tissue culture for the delivery of gene editing reagents limits the application of gene editing to commercially relevant varieties of many crop species. To overcome this bottleneck, plant RNA viruses have been deployed as versatile tools for in planta delivery of recombinant RNA. Viral delivery of single‐guide RNAs (sgRNAs) to transgenic plants that stably express CRISPR‐associated (Cas) endonuclease has been successfully used for targeted mutagenesis in several dicotyledonous and few monocotyledonous plants. Progress with this approach in monocotyledonous plants is limited so far by the availability of effective viral vectors. We engineered a set of foxtail mosaic virus (FoMV) and barley stripe mosaic virus (BSMV) vectors to deliver the fluorescent protein AmCyan to track viral infection and movement in Sorghum bicolor . We further used these viruses to deliver and express sgRNAs to Cas9 and Green Fluorescent Protein (GFP) expressing transgenic sorghum lines, targeting Phytoene desaturase ( PDS ), Magnesium‐chelatase subunit I ( MgCh ), 4‐hydroxy‐3‐methylbut‐2‐enyl diphosphate reductase , orthologs of maize Lemon white1 ( Lw1 ) or GFP . The recombinant BSMV did neither infect sorghum nor deliver or express AmCyan and sgRNAs. In contrast, the recombinant FoMV systemically spread throughout sorghum plants and induced somatic mutations with frequencies reaching up to 60%. This mutagenesis led to visible phenotypic changes, demonstrating the potential of FoMV for in planta gene editing and functional genomics studies in sorghum.

54 ENVIRONMENTAL SCIENCES↗