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At least 775 records · Page 43

193 nm Ultraviolet Photodissociation for the Characterization of Singly Charged Proteoforms Generated by MALDI

MALDI imaging allows for the near-cellular profiling of proteoforms directly from microbial, plant, and mammalian samples. Despite detecting hundreds of proteoforms, identification of unknowns with only intact mass information remains a distinct challenge, even with high mass resolving power and mass accuracy. To this end, many supplementary methods have been used to create experimental databases for accurate mass matching, including bulk or spatially resolved bottom-up and/or top-down proteomics. Herein, we describe the application of 193 nm ultraviolet photodissociation (UVPD) for fragmentation of quadrupole isolated singly charged ubiquitin (m/z 8565) by MALDI-UVPD on a UHMR HF Orbitrap. This platform permitted the high-resolution accurate mass measurement of not just terminal fragments but also large internal fragments. Finally, the outlined workflow demonstrates the feasibility of top-down analyses of isolated MALDI protein ions and the potential toward more comprehensive characterization of proteoforms in MALDI imaging applications.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

VLBI2010 Imaging and Structure Corrections

Simulations show that the next generation VLBI system is generally well suited for imaging extragalactic radio sources. In addition to revealing the morphology of the sources, simulated VLBI2010 images may also be used to generate structure correction maps, which characterize the impact of source structure on the VLBI measurements. By comparing structure corrections for a set of simulated images based on Monte-Carlo generated visibilities with theoretical structure corrections derived from the model, we assess the accuracy of VLBI2010 structure corrections. In the most favorable case (32-station network, source at declination +40 deg.), statistics demonstrate that the structure corrections obtained from simulated images are statistically close to the theoretical model ones, which seems very promising for the VLBI2010 system.

Collioud, Arnaud↗

Chimera Grid Tools

Chimera Grid Tools (CGT) is a software package for performing computational fluid dynamics (CFD) analysis utilizing the Chimera-overset-grid method. For modeling flows with viscosity about geometrically complex bodies in relative motion, the Chimera-overset-grid method is among the most computationally cost-effective methods for obtaining accurate aerodynamic results. CGT contains a large collection of tools for generating overset grids, preparing inputs for computer programs that solve equations of flow on the grids, and post-processing of flow-solution data. The tools in CGT include grid editing tools, surface-grid-generation tools, volume-grid-generation tools, utility scripts, configuration scripts, and tools for post-processing (including generation of animated images of flows and calculating forces and moments exerted on affected bodies). One of the tools, denoted OVERGRID, is a graphical user interface (GUI) that serves to visualize the grids and flow solutions and provides central access to many other tools. The GUI facilitates the generation of grids for a new flow-field configuration. Scripts that follow the grid generation process can then be constructed to mostly automate grid generation for similar configurations. CGT is designed for use in conjunction with a computer-aided-design program that provides the geometry description of the bodies, and a flow-solver program.

Chan, William M.↗

Highly versatile computer-controlled television detector system

A description is presented of a television detector system which has been designed to accommodate a wide range of applications. It is currently being developed for use in X-ray diffraction, X-ray astrophysics, and electron microscopy, but it is also well suited for astronomical observations. The image can be integrated in a large, high-speed memory system, in the memory of a computer system, or the target of the TV tube or CCD array. The detector system consists of a continuously scanned, intensified SIT vidicon with scan and processing electronics which generate a digital image that is integrated in the detector memory. Attention is given to details regarding the camera system, scan control and image processing electronics, the memory system, and aspects of detector performance.

Kalata, K.↗

Improvement of CAT scanned images

Digital enhancement procedure improves definition of images. Tomogram is generated from large number of X-ray beams. Beams are collimated and small in diameter. Scanning device passes beams sequentially through human subject at many different angles. Battery of transducers opposite subject senses attenuated signals. Signals are transmitted to computer where they are used in construction of image on transverse plane through body.

Roberts, E., Jr.↗

Simulating Visible/Infrared Imager Radiometer Suite Normalized Difference Vegetation Index Data Using Hyperion and MODIS

The success of MODIS (the Moderate Resolution Imaging Spectrometer) in creating unprecedented, timely, high-quality data for vegetation and other studies has created great anticipation for data from VIIRS (the Visible/Infrared Imager Radiometer Suite). VIIRS will be carried onboard the joint NASA/Department of Defense/National Oceanic and Atmospheric Administration NPP (NPOESS (National Polar-orbiting Operational Environmental Satellite System) Preparatory Project). Because the VIIRS instruments will have lower spatial resolution than the current MODIS instruments 400 m versus 250 m at nadir for the channels used to generate Normalized Difference Vegetation Index data, scientists need the answer to this question: how will the change in resolution affect vegetation studies? By using simulated VIIRS measurements, this question may be answered before the VIIRS instruments are deployed in space. Using simulated VIIRS products, the U.S. Department of Agriculture and other operational agencies can then modify their decision support systems appropriately in preparation for receipt of actual VIIRS data. VIIRS simulations and validations will be based on the ART (Application Research Toolbox), an integrated set of algorithms and models developed in MATLAB(Registerd TradeMark) that enables users to perform a suite of simulations and statistical trade studies on remote sensing systems. Specifically, the ART provides the capability to generate simulated multispectral image products, at various scales, from high spatial hyperspectral and/or multispectral image products. The ART uses acquired ( real ) or synthetic datasets, along with sensor specifications, to create simulated datasets. For existing multispectral sensor systems, the simulated data products are used for comparison, verification, and validation of the simulated system s actual products. VIIRS simulations will be performed using Hyperion and MODIS datasets. The hyperspectral and hyperspatial properties of Hyperion data will be used to produce simulated MODIS and VIIRS products. Hyperion-derived MODIS data will be compared with near-coincident MODIS collects to validate both spectral and spatial synthesis, which will ascertain the accuracy of converting from MODIS to VIIRS. MODIS-derived VIIRS data is needed for global coverage and for the generation of time series for regional and global investigations. These types of simulations will have errors associated with aliasing for some scene types. This study will help quantify these errors and will identify cases where high-quality, MODIS-derived VIIRS data will be available.

Ross, Kenton W.↗

Structural Anomalies Detected in Ceramic Matrix Composites Using Combined Nondestructive Evaluation and Finite Element Analysis (NDE and FEA)

Most reverse engineering approaches involve imaging or digitizing an object and then creating a computerized reconstruction that can be integrated, in three dimensions, into a particular design environment. The rapid prototyping technique builds high-quality physical prototypes directly from computer-aided design files. This fundamental technique for interpreting and interacting with large data sets is being used here via Velocity2 (an integrated image-processing software, ref. 1) using computed tomography (CT) data to produce a prototype three-dimensional test specimen model for analyses. A study at the NASA Glenn Research Center proposes to use these capabilities to conduct a combined nondestructive evaluation (NDE) and finite element analysis (FEA) to screen pretest and posttest structural anomalies in structural components. A tensile specimen made of silicon nitrite (Si3N4) ceramic matrix composite was considered to evaluate structural durability and deformity. Ceramic matrix composites are being sought as candidate materials to replace nickel-base superalloys for turbine engine applications. They have the unique characteristics of being able to withstand higher operating temperatures and harsh combustion environments. In addition, their low densities relative to metals help reduce component mass (ref. 2). Detailed three-dimensional volume rendering of the tensile test specimen was successfully carried out with Velocity2 (ref. 1) using two-dimensional images that were generated via computed tomography. Subsequent, three-dimensional finite element analyses were performed, and the results obtained were compared with those predicted by NDE-based calculations and experimental tests. It was shown that Velocity2 software can be used to render a three-dimensional object from a series of CT scan images with a minimum level of complexity. The analytical results (ref. 3) show that the high-stress regions correlated well with the damage sites identified by the CT scans and the experimental data. Furthermore, modeling of the voids collected via NDE offered an analytical advantage that resulted in more accurate assessments of the material s structural strength. The top figure shows a CT scan image of the specimen test section illustrating various hidden structural entities in the material and an optical image of the test specimen considered in this study. The bottom figure represents the stress response predicted from the finite element analyses (ref .3 ) for a selected CT slice where it clearly illustrates the correspondence of the high stress risers due to voids in the material with those predicted by the NDE. This study is continuing, and efforts are concentrated on improving the modeling capabilities to imitate the structural anomalies as detected.

Abdul-Aziz, Ali↗

Dispersed Fringe Sensing Analysis - DFSA

Dispersed Fringe Sensing (DFS) is a technique for measuring and phasing segmented telescope mirrors using a dispersed broadband light image. DFS is capable of breaking the monochromatic light ambiguity, measuring absolute piston errors between segments of large segmented primary mirrors to tens of nanometers accuracy over a range of 100 micrometers or more. The DFSA software tool analyzes DFS images to extract DFS encoded segment piston errors, which can be used to measure piston distances between primary mirror segments of ground and space telescopes. This information is necessary to control mirror segments to establish a smooth, continuous primary figure needed to achieve high optical quality. The DFSA tool is versatile, allowing precise piston measurements from a variety of different optical configurations. DFSA technology may be used for measuring wavefront pistons from sub-apertures defined by adjacent segments (such as Keck Telescope), or from separated sub-apertures used for testing large optical systems (such as sub-aperture wavefront testing for large primary mirrors using auto-collimating flats). An experimental demonstration of the coarse-phasing technology with verification of DFSA was performed at the Keck Telescope. DFSA includes image processing, wavelength and source spectral calibration, fringe extraction line determination, dispersed fringe analysis, and wavefront piston sign determination. The code is robust against internal optical system aberrations and against spectral variations of the source. In addition to the DFSA tool, the software package contains a simple but sophisticated MATLAB model to generate dispersed fringe images of optical system configurations in order to quickly estimate the coarse phasing performance given the optical and operational design requirements. Combining MATLAB (a high-level language and interactive environment developed by MathWorks), MACOS (JPL s software package for Modeling and Analysis for Controlled Optical Systems), and DFSA provides a unique optical development, modeling and analysis package to study current and future approaches to coarse phasing controlled segmented optical systems.

Sigrist, Norbert↗

Concept Lens: Visual Comparison and Evaluation of Generative Model Manipulations

Generative models are becoming a transformative technology for the creation and editing of images. However, it remains challenging to harness these models for precise image manipulation. These challenges often manifest as inconsistency in the editing process, where both the type and amount of semantic change, depend on the image being manipulated. Moreover, there exist many methods for computing image manipulations, whose development is hindered by the matter of inconsistency. This paper aims to address these challenges by improving how we evaluate, compare, and explore the space of manipulations offered by a generative model. We present Concept Lens, a visual interface that is designed to aid users in understanding semantic concepts carried in image manipulations, and how these manipulations vary over generated images. Given the large space of possible images produced by a generative model, Concept Lens is designed to support the exploration of both generated images, and their manipulations, at multiple levels of detail. To this end, the layout of Concept Lens is informed by two hierarchies: a hierarchical organization of (1) original images, grouped by their similarities, and (2) image manipulations, where manipulations that induce similar changes are grouped together. This layout allows one to discover the types of images that consistently respond to a group of manipulations, and vice versa, manipulations that consistently respond to a group of codes. We show the benefits of this design across multiple use cases, specifically, studying the quality of manipulations for a single method, and offering a means of comparing different methods.

clustering↗

Wavefront distortion correction in scanning tunneling microscope image

Here, we report an algorithm to identify and correct distorted wavefronts in atomic resolution scanning tunneling microscope images. This algorithm can be used to correct nonlinear in-plane distortions without prior knowledge of the physical scanning parameters, the characteristics of the piezoelectric actuator, or individual atom positions. The 2D image is first defined as a sum of sinusoidal plane waves, where a nonlinear distortion renders a curve for an otherwise ideal linear wavefront. Using the Fourier transforms of local areas of the image, the algorithm generates a wavefront vector field. The identified wavefronts are subsequently linearized for each plane wave without changing lattice orders, giving rise to distortion corrections. Our algorithm is complementary to conventional post-processing algorithms that require prior detection of real space features, which can also be used to correct nonlinear distortions in 2D images acquired by other microscopy techniques.

47 OTHER INSTRUMENTATION↗

Hyperspectral acquisition with ScanImage at the single pixel level: application to time domain coherent Raman imaging

We present a comprehensive strategy and its practical implementation using the commercial ScanImage software platform to perform hyperspectral point scanning microscopy when a fast time-dependent signal varies at each pixel level. In the proposed acquisition scheme, the scan along the X-axis is slowed down while the data acquisition is maintained at a high pace to enable the rapid acquisition of the time-dependent signal at each pixel level. The ScanImage generated raw 2D images have a very asymmetric aspect ratio between X and Y, the X axis encoding both for space and time acquisition. The results are X-axis macro-pixel where the associated time-dependent signal is sampled to provide hyperspectral information. We exemplified the proposed hyperspectral scheme in the context of time-domain coherent Raman imaging, where a pump pulse impulsively excites molecular vibrations that are subsequently probed by a time-delayed probe pulse. In this case, the time-dependent signal is a fast acousto-optics delay line that can scan a delay of 4.5ps in 25 μ s at each pixel level. With this acquisition scheme, we demonstrate ultra-fast hyperspectral vibrational imaging in the low frequency range [10 cm −1 , 150 cm −1 ] over a 500 μm field of view (64 x 64 pixels) in 130ms (∼ 7.5 frames/s). The proposed acquisition scheme can be readily extended to other applications requiring the acquisition of a fast-evolving signal at each pixel level.

Metais, Samuel↗

Landsat image registration - A study of system parameters

Some applications of Landsat data, particularily agricultural and forestry applications, require the ability to geometrically superimpose or register data acquired at different times and possibly by different satellites. An experimental investigation relating to a registration processor used by the Johnson Space Center for this purpose is the subject of this paper. Correlation of small subareas of images is at the heart of this registration processor and the manner in which various system parameters affect the correlation process is the prime area of investigation. Parameters investigated include preprocessing methods, methods for detecting sucessful correlations, fitting a surface to the correlation patch, fraction of pixels designated as edge pixels in edge detection adn local versus global generation of edge images. A suboptimum search procedure is used to find a good parameter set for this registration processor.

Wacker, A. G.↗

Specifications of EBR-II Neutron Radiography Method Description and Digitization Approach

All neutron radiography (NRAD) images of fuel pins in Argonne’s collection were originally generated using the NRAD imaging facility established in the Hot Fuel Examination Facility (HFEF) at Idaho National Laboratory (INL). The NRAD reactor facility was built in 1977 and has been operating since. The reactor is a TRIGA-type reactor operating at a power level of 250 kWth to provide a neutron source for radiography imaging. The reactor is equipped with two beam tubes (i.e., east beam tube and north beam tube) to guide the neutron beams to two radiography stations. The east radiography station is directly under the HFEF main cell and is dedicated for specimens already in the HFEF hot cell. The north radiography station is outside of the main HFEF hot cell and allows NRAD imaging of non-irradiated items. The NRAD images of EBR-II irradiated metallic fuel pins were taken in the east radiography station. Thermal neutrons have the capability to transmit through most materials and are ideal for NRAD imaging. However, because of their high thermal neutron absorption cross-section, fissile materials (e.g., highly-enriched nuclear fuels) may not be as transmissible to thermal neutrons. This is also the case for oversize specimens with extraneous thickness. Epithermal neutron imaging is therefore used as a complement to thermal neutron NRAD imaging. At HFEF’s NRAD facility, both thermal and epithermal neutrons can be used for NRAD imaging. Irradiated nuclear fuels emit high levels of γ radiation that can easily darken X-ray films, so direct exposure NRAD cannot be used to image them. Instead, an indirect NRAD imaging method was developed at HFEF’s NRAD facility. In this method, foils made of materials that can be activated by neutrons (i.e., with large neutron absorption cross section) are used to collect transmitted neutron signals. Then the activated foils are then placed against X-ray films and enclosed in a vacuum cassette so that the γ decay from the activated foils can produce images on the X-ray films. Then, general X-ray film processing procedures are used to digitize and store the images. By using different foil materials, different energy neutrons can be used for NRAD imaging. At the HFEF NRAD station, two types of films are commonly used: dysprosium (Dy) foils with thickness of 130 microns are used to capture thermal neutron signal, while indium (In) foils with thickness of 130 microns are used to capture epithermal neutron signal. A cadmium or gadolinium foil is put before the indium foil to work as a thermal neutron filter. The thermal and epithermal NRAD images can be taken simultaneously by using a Dy/Cd/In sandwiched foil combination. The typical NRAD exposure time is approximately 20 minutes. Then the exposed foils are transferred to film vacuum cassettes. The vacuum ensures that there is no gap between the foil and the film. The foil-to-film exposure time is at least three half-lives of the corresponding radioisotopes, which are 3 hours for In and 7.5 hours for Dy, respectively. Exposed films are processed using an automatic film processor to produce completed NRAD images.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Interactive Image-Analysis Program

BLOBTOOL computer program used to prepare image for analysis and generates various quantitative measurements of objects or structures present in images. Includes basic input/output capabilities, option to display built-in color tables, and access to on-line help. Written in C Language.

Leonard, Desiree M.↗

Techniques and Tools for Estimating Ionospheric Effects in Interferometric and Polarimetric SAR Data

The InSAR Scientific Computing Environment (ISCE) is a flexible, extensible software tool designed for the end-to-end processing and analysis of synthetic aperture radar data. ISCE inherits the core of the ROI_PAC interferometric tool, but contains improvements at all levels of the radar processing chain, including a modular and extensible architecture, new focusing approach, better geocoding of the data, handling of multi-polarization data, radiometric calibration, and estimation and correction of ionospheric effects. In this paper we describe the characteristics of ISCE with emphasis on the ionospheric modules. To detect ionospheric anomalies, ISCE implements the Faraday rotation method using quadpolarimetric images, and the split-spectrum technique using interferometric single-, dual- and quad-polarimetric images. The ability to generate co-registered time series of quad-polarimetric images makes ISCE also an ideal tool to be used for polarimetric-interferometric radar applications.

Ionosphere↗

The generation and use of numerical shape models for irregular Solar System objects

We describe a procedure that allows the efficient generation of numerical shape models for irregular Solar System objects, where a numerical model is simply a table of evenly spaced body-centered latitudes and longitudes and their associated radii. This modeling technique uses a combination of data from limbs, terminators, and control points, and produces shape models that have some important advantages over analytical shape models. Accurate numerical shape models make it feasible to study irregular objects with a wide range of standard scientific analysis techniques. These applications include the determination of moments of inertia and surface gravity, the mapping of surface locations and structural orientations, photometric measurement and analysis, the reprojection and mosaicking of digital images, and the generation of albedo maps. The capabilities of our modeling procedure are illustrated through the development of an accurate numerical shape model for Phobos and the production of a global, high-resolution, high-pass-filtered digital image mosaic of this Martian moon. Other irregular objects that have been modeled, or are being modeled, include the asteroid Gaspra and the satellites Deimos, Amalthea, Epimetheus, Janus, Hyperion, and Proteus.

Simonelli, Damon P.↗