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At least 775 records · Page 43

An ML-based terrestrial data fusion and augmentation framework to enable advanced understanding of the terrestrial carbon and water interactions

Soil moisture is essential to the terrestrial carbon and water cycles and land–atmosphere interactions. There are various types of soil moisture data, and each type has the distinct spatiotemporal strengths and limitations, depending on the diverse applications and retrieval methodologies of different data types (Li et al., in review; The PNNL-82151 FY23 Report). However, the limitations of different soil moisture data in terms of accuracy and spatiotemporal coverage hinder our ability to further understand the soil moisture dynamics across scales. To have a gap free soil moisture data product with a fine spatiotemporal coverage and vertical profiles, we train extreme gradient boosting (XGBoost) models by using (1) in-situ soil moisture measurements from the International Soil Moisture Network (ISMN), (2) soil moisture from the ECMWF reanalysis (ERA) at the 9 km and sub-daily spatiotemporal resolution, (3) the Daymet meteorological fields, and (4) data products that characterize surface conditions, including soil texture, organic content, topography, vegetation type, and rooting depth. We use the trained XGBoost models that have consistent performance across seven soil layers, i.e., 0–5 cm, 5–10 cm, 10–20 cm, 20–40 cm, 40–60 cm, 60–100 cm, and 100–200 cm, and the gridded model predictors to generate a soil moisture data at the 1 km and daily spatiotemporal resolution for the Continental United States (CONUS) from 2001–2020. This dataset can be broadly used for Earth system model benchmark, monitoring extreme weathers, making informed decisions regarding agriculture, water resource management, climate change mitigation, and ecosystem preservation.

58 GEOSCIENCES↗

Changes of Soil Bacterial Diversity as a Consequence of Agricultural Land Use in a Semi-Arid Ecosystem

Natural scrublands in semi-arid deserts are increasingly being converted into fields. This results in losses of characteristic flora and fauna, and may also affect microbial diversity. In the present study, the long-term effect (50 years) of such a transition on soil bacterial communities was explored at two sites typical of semi-arid deserts. Comparisons were made between soil samples from alfalfa fields and the adjacent scrublands by two complementary methods based on 16S rRNA gene fragments amplified from total community DNA. Denaturing gradient gel electrophoresis (DGGE) analyses revealed significant effects of the transition on community composition of Bacteria, Actinobacteria, Alpha- and Betaproteobacteria at both sites. PhyloChip hybridization analysis uncovered that the transition negatively affected taxa such as Acidobacteria, Chloroflexi, Acidimicrobiales, Rubrobacterales, Deltaproteobacteria and Clostridia, while Alpha-, Beta- and Gammaproteobacteria, Bacteroidetes and Actinobacteria increased in abundance. Redundancy analysis suggested that the community composition of phyla responding to agricultural use (except for Spirochaetes) correlated with soil parameters that were significantly different between the agricultural and scrubland soil. The arable soils were lower in organic matter and phosphate concentration, and higher in salinity. The variation in the bacterial community composition was higher in soils from scrubland than from agriculture, as revealed by DGGE and PhyloChip analyses, suggesting reduced beta diversity due to agricultural practices. The long-term use for agriculture resulted in profound changes in the bacterial community and physicochemical characteristics of former scrublands, which may irreversibly affect the natural soil ecosystem.

54 ENVIRONMENTAL SCIENCES↗

Calibrated Hydrothermal Parameters, Barrow, Alaska, 2013

A model-observation-experiment process (ModEx) is used to generate three 1D models of characteristic micro-topographical land-formations, which are capable of simulating present active layer thickness (ALT) from current climate conditions. Each column was used in a coupled calibration to identify moss, peat and mineral soil hydrothermal properties to be used in up-scaled simulations. Observational soil temperature data from a tundra site located near Barrow, AK (Area C) is used to calibrate thermal properties of moss, peat, and sandy loam soil to be used in the multiphysics Advanced Terrestrial Simulator (ATS) models. Simulation results are a list of calibrated hydrothermal parameters for moss, peat, and mineral soil hydrothermal parameters drawn from literature and field observations at the NGEE-Arctic site. Find more details in the associated publication by Atchley, et.al, 2015.There is one data file as both a .xlsx and .csv. The datafile was transformed into a csv for interoperability, however some information might have been lost in the transformation.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Inter-Kingdom Viral Interactions

Please cite as : Josué A. Rodríguez-Ramos, Amy E. Zimmerman, Ruonan Wu, Sheryl Bell, Trinidad Alfaro, Kirsten Hofmockel, William C. Nelson. 2025. Inter-Kingdom Viral Interactions. [Data Set] PNNL DataHub. This data is published under a CC0 license. The authors encourage data reuse and request attribution by referencing the above citations for the data package and associated manuscript. Deciphering viral ecology in soils is challenging due to their high physiochemical and community complexity. To enhance detection of sub-communities of DNA and RNA viruses, we applied fractionation approaches to soils collected across a moisture gradient from a grassland field experiment. Analyses included metagenomics and metatranscriptomics of size-fractionated extracellular viruses (i.e., DNA and RNA viromes), metagenomics of bacteria/archaea- or eukaryote-enriched samples, and whole soil metatranscriptomes with rRNA-depletion or polyadenylation enrichment. While RNA virome and whole soil RNA methods captured similar viral diversity, RNA viromes identified longer, higher-quality genomes. Further, we showed that significantly more DNA viruses were active in higher moisture than lower moisture samples, whereas responses by overall diversity vary by genome type (DNA versus RNA genomes). Finally, we demonstrate the power of fractionation approaches for identifying distinct viral communities that infect unique hosts, which has significant implications for ecological investigations, particularly related to interkingdom interactions.

59 BASIC BIOLOGICAL SCIENCES↗

Integrated Landscape Management to Reduce Biomass Feedstock Access Costs

Using geospatial, machine learning, and optimization techniques, a field scoring and design framework known as the bioenergy Landscape Environmental Assessment and Design System (bioLEADS) was developed and used to show through modelling that it is possible to reduce biomass feedstock access costs, improve field revenue, and shift intensive row crop production away from subfield areas susceptible to erosion and low in soil organic carbon. The purpose of bioLEADS is to automate agricultural field selection and subfield allocation to perennial energy crop production while incorporating reduced biomass feedstock access costs to support attaining the U.S. Department of Energy (DOE) Bioenergy Technologies Office (BETO) cost targets.

09 BIOMASS FUELS↗

A Model for Backscattering from Quasi Periodic Corn Canopies at L-Band

In this study, a model for backscattering at L-band from a corn canopy is proposed. The canopy consists of a quasi-periodic distribution of stalks and a random distribution of leaves. The Distorted Born Approximation (DBA) is employed to calculate the single scattered return from the corn field. The new feature of the method is that the coherence of the stalks in the row direction is incorporated in the model in a systematic fashion. Since the wavelength is on the order of the distance between corn stalks in a row, grating lobe behavior is observed at certain azimuth angles of incidence. The results are compared with experimental values measured in Huntsville, Alabama in 1998. The mean field and the effective dielectric constant of the canopy are obtained by using the Foldy approximation. The stalks are placed in the effective medium in a two dimensional lattice to simulate the row structure of a corn field. In order to mimic a real corn field, a quasi-periodic stalk distribution is assumed where the stalks are given small random perturbations about their lattice locations. Corn leaves are also embedded in the effective medium and the backscattered field from the stalks and the leaves is computed. The backscattering coefficient is calculated and averaged over successive stalk position perturbations. It is assumed that soil erosion has smoothed the soil sufficiently so that it can be assumed flat. Corn field backscatter data was collected from cornfields during the Huntsville 98 experimental campaign held at Alabama A&M University Research Station, Huntsville, Alabama in 1998 using the NASA/GW truck mounted radar. Extensive ground truth data was collected. This included soil moisture measurements and corn plant architectural data to be used in the model. In particular, the distances between the stalks in a single row have been measured. The L-band radar backscatter data was collected for both H and V polarizations and for look angles of 15o and 45o over a two week period under varying soil moisture conditions. These measured backscattering values will be compared with the model backscattering values and a discussion of the results will be presented.

Lang, R.↗

Regulator Interface Strategies Implemented at the Y-12 National Security Complex Old Salvage Yard Soils Remediation Project, Oak Ridge, TN - 12162

The Oak Ridge Y-12 National Security Complex housed an area known as the Old Salvage Yard (OSY) that was approximately 7 acres. The OSY was used as an area for the accumulation, processing and storage of scrap metal and equipment from Y-12 operations extending from 1968 until 2009. Areas in the northern sections of OSY also have been used for the storage of used oils containing solvents and the accumulation and recycling or de-heading and crushing of 55-gal metal drums. Scrap metal operations historically involved the accumulation, sorting, storage, public sale or disposal of scrap metal and equipment. Non-containerised storage of scrap metal was routine until 1995 when scrap metal received at OSY was placed in B-24 and B-25 boxes. Under the American Recovery and Reinvestment Act (ARRA), approximately 26,759 cubic meters of scrap metal and debris were removed and disposed at both on and off-site disposal facilities including the on-site, Oak Ridge Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) landfill in 2010 and 2011. This removal action was performed in accordance with a CERCLA Record of Decision (ROD) and a close working relationship with both the U.S. Environmental Protection Agency (EPA) Region IV and Tennessee Department of Environment and Conservation (TDEC). Due to efficiencies and the excellent cooperative relationship forged with EPA Region IV and TDEC for Y-12 ARRA Cleanup Projects, a surplus of funding was available for additional remediation work that was completed in fiscal year (FY) 2011. The underlying OSY soils were targeted for characterization and potential remediation. To expedite these important activities, the U.S. Department of Energy Oak Ridge Environmental Management partnered with the regulators during detailed planning sessions through a variety of means to quickly and efficiently characterize and pinpoint areas requiring remediation according to previous ROD commitments. Data Quality Objectives (DQOs), data-sharing, real-time characterization reporting, surface and groundwater modeling and other interface planning activities were utilized to help facilitate and complete characterization and remediation activities. As a result of these strategies, the surgical extraction of one contiguous area of soil approximately 354 cubic meters is planned for FY12. The strategies discussed resulted in a major reduction of footprint remediation (i.e., 2.8% of the original estimate) which was originally estimated at over 26,759 cubic meters. The original estimate was developed using historical data collected at various times over the period of 20 years. By leveraging a hybrid sampling approach that involved both statistically-based and biased sampling locations, the area of contamination was significantly reduced resulting in both a compliant remedial design that is cost effective while mitigating a principle threat sources to surface and groundwater at the Y-12 plant. One remedial action boundary of 354 cubic meters was verified in the northern section of the Western OSY area known as the old drum de-header station for VOCs. The original estimate for disposal was in excess of 26,759 cubic meters. This area is scheduled for waste characterization and profile development in the first half of fiscal year 2012. The anticipated disposal facility is an on-site Oak Ridge CERCLA disposal landfill known as the Environmental Management Waste Management Facility (EMWMF). By utilizing the careful strategic planning, field-based screening and close cooperation of regulatory stakeholders as detailed in this paper, the total area of soil requiring remedial action within the Y-12 OSY footprint was 354 cubic meters or 2.8% of the original planned estimate. A potential waste reduction of 97.2% was realized over the original planned estimate for OSY Soils. Significant cost savings were achieved by - Minimizing the footprint of the remedial action; - Confirmatory analysis of soils instead of use of historical sampling results for waste profile development; - Targeting traditional laboratory analysis for soils that failed field screen protocols for radionuclides, heavy metals and VOCs; - Providing accurate and precise definition of RABs; and - Reduction of target contaminants for focused laboratory analysis instead of broad-range contaminants. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

The response of soil carbon storage and microbially mediated carbon turnover to simulated climatic disturbance in a northern peatland forest. Revisiting the concept of soil organic matter recalcitrance

The goal of this project was to investigate changes in the structure of dissolved and solid phase organic matter, the production of CO 2 and CH 4 , and the composition of decomposer microbial communities in response to the climatic forcing of environmental processes that determine the balance between carbon gas production versus storage and sequestration in peatlands. Cutting-edge analytical chemistry and next generation sequencing of microbial genes were been applied to habitats at the Marcell Experimental Forest (MEF), where the US DOE’s Oak Ridge National Laboratory and the USDA Forest Service are constructing a large-scale ecosystem study entitled, “Spruce and Peatland Responses Under Climatic and Environmental Change”(SPRUCE). Our study represented a comprehensive characterization of the sources, transformation, and decomposition of organic matter in the S1 bog at MEF. Multiple lines of evidence point to distinct, vertical zones of organic matter transformation: 1) the acrotelm consisting of living mosses, root material, and newly formed litter (0-30 cm), 2) the mesotelm, a mid-depth transition zone (30-75 cm) characterized by labile organic C compounds and intense decomposition, and 3) the underlying catotelm (below 75cm) characterized by refractory organic compounds as well as relatively low decomposition rates. These zones are in part defined by physical changes in hydraulic conductivity and water table depth. O-alkyl-C, which represents the carbohydrate fraction in the peat, was shown to be an excellent proxy for soil decomposition rates. The carbon cycle in deep peat was shown to be fueled by modern carbon sources further indicating that hydrology and surface vegetation play a role in belowground carbon cycling. We provide the first metagenomic study of an ombrotrophic peat bog, with novel insights into microbial specialization and functions in this unique terrestrial ecosystem. Vertical structuring of microbial communities closely paralleled the chemical evolution of peat, with large shifts in microbial populations occurring in the biogeochemical hotspot, the mesotelm, where the highest rates of decomposition were detected. Stable isotope geochemistry and potential rates of methane production paralleled vertical changes in methanogen community composition to indicate a predominance of acetoclastic methanogenesis mediated by the Methanosarcinales in the mesotelm, while hydrogen-utilizing methanogens dominated in the deeper catotelm. Evidence pointed to the availability of phosphorus as well as nitrogen limiting the microbially-mediated turnover of organic carbon at MEF. Prior to initiation of the experimental treatments, our study provided key baseline data for the SPRUCE site on the vertical stratification of peat decomposition, key enzymatic pathways, and microbial taxa containing these pathways. The sensitivity of soil carbon turnover to climate change is strongly linked to recalcitrant carbon stocks and the temperature sensitivity of decomposition is thought to increase with increasing molecular complexity of carbon substrates. This project delivered results on how climate change perturbations impact the microbially-mediated turnover of recalcitrant organic matter in peatland forest soils, both under controlled conditions in the laboratory and at the ecosystem-scale in the field. This project revisited the concept of “recalcitrance” in the regulation of soil carbon turnover using a combination of natural abundance radiocarbon and optical spectroscopic measurements on bulk DOM, and high resolution molecular characterization of DOM. The project elucidated how organic matter reactivity and decomposition will respond to climate change in a both a qualitative (organic matter lability) and quantitiative (increased rates) manner. An Aromaticity Index was developed to represent a more direct and accurate parameter for modeling of DOM reactivity in peatlands. The abundance and community composition of soil microorganisms that mediate C cycling were interrogated with depth in the peat, with season, and in manipulated climate enclosures at unprecedented resolution. Therefore this project delivered strategic new insights on the functioning of peatland ecosystems that collectively store approximately one-third of the world's soil carbon. Furthermore, results from the detailed characterization of DOM lability and microbial community structure/ function will be employed to further develop biogeochemical models to include microbial respiration pathways as well as to track carbon flow with a term that incorporates relative reactivity based on aromaticity index. As it stands now, detailed soil organic matter structure and microbial parameters are not included in Earth system models.

54 ENVIRONMENTAL SCIENCES↗

Polarimetric Scanning Radiometer C and X Band Microwave Observations During SMEX03

Soil Moisture Experiments 2003 (SMEX03) was the second in a series of field campaigns using the NOAA Polarimetric Scanning Radiometer (PSR/CX) designed to validate brightness temperature data and soil moisture retrieval algorithms for the Advanced during SMEX03 were: calibration and validation of AMSR-E brightness temperature observations over different climate/vegetation regions of the US. (Alabama, Georgia, Oklahoma), identification of possible sources of Radio Frequency Interference (RFI), comparison of X-band observations from TRMM Microwave Imager (TMI), AMSR-E and PSR/CX, and exploring the potential of soil moisture retrieval algorithms using C and X band imagery in diverse landscapes. In the current investigation, more than one hundred flightlines of PSR/CX data were extensively processed to produce gridded brightness temperature products for the four study regions. Variations associated with soil moisture were not as large as hoped for due to the lack of significant rainfall in Oklahoma. Observations obtained over Alabama include a wide range of soil moisture and vegetation conditions for C and X band frequencies. These results clearly showed a lack of sensitivity to rainfall/soil moisture under forest canopy cover. Quantitative comparisons made between the PSR/CX, AMSR-E for validated that both the PSR/CX and AMSR-E data were well calibrated. X band comparisons of the PSR/CX high resolution and AMSR-E and TMI low-resolution data indicated a linear scaling for the range of conditions studied in SMEX03. These results will form the basis for further soil moisture investigations.

Jackson, Thomas J.↗

Shallow Soil Polychlorinated Biphenyl Site Assessment Report

This report presents a summary of the shallow soil polychlorinated biphenyl (PCB) site assessment activities that occurred from December 2020 through July 2022 at General Services Administration Reclamation Yard, Solid Waste Management Unit (SWMU) 010, located at the John F. Kennedy Space Center (KSC), Florida. The site is monitored under KSC’s Resource Conservation and Recovery Act Corrective Action Program, which also meets the requirements of Chapter 62-780, Florida Administrative Code. For the purposes of this report, three separate shallow soil plumes, known as the Northeast Shallow Soil PCB Plume, Southeast Shallow Soil PCB Plume, and West Shallow Soil PCB Plume, were identified for this site. The activities presented in this report include seven field events conducted between December 2020 and July 2022, which include shallow soil sample collection in the Northeast, Southeast, and West Shallow Soil PCB Plumes. AECOM Technical Services, Inc., personnel collected 330 soil samples from 118 boring locations. The samples were collected at 0.5-foot depth intervals to various depths based on analytical results and depth to groundwater. The soil samples were submitted to a fixed-based laboratory for analysis by United States Environmental Protection Agency Method 8082A for total PCBs. Several soil samples had PCB concentrations above the State of Florida Direct Exposure Residential and Industrial Soil Cleanup Target Levels (SCTLs), and two soil samples had PCB concentrations above the State of Florida Leachability for Groundwater. No soil borings were located beneath impervious surfaces, such as concrete or asphalt. The current Land Use Control Implementation Plan (LUCIP) covers PCBs in soils above the residential SCTL, which includes the paved areas.

PCBs↗

Shallow Soil Polychlorinated Biphenyl Site Assessment Report: General Services Administration Reclamation Yard Solid Waste Management Unit 010

This report presents a summary of the shallow soil polychlorinated biphenyl (PCB) site assessment activities that occurred from December 2020 through July 2022 at General Services Administration Reclamation Yard, Solid Waste Management Unit (SWMU) 010, located at the John F. Kennedy Space Center (KSC), Florida. The site is monitored under KSC’s Resource Conservation and Recovery Act Corrective Action Program, which also meets the requirements of Chapter 62-780, Florida Administrative Code. For the purposes of this report, three separate shallow soil plumes, known as the Northeast Shallow Soil PCB Plume, Southeast Shallow Soil PCB Plume, and West Shallow Soil PCB Plume, were identified for this site. The activities presented in this report include seven field events conducted between December 2020 and July 2022, which include shallow soil sample collection in the Northeast, Southeast, and West Shallow Soil PCB Plumes. AECOM Technical Services, Inc., personnel collected 330 soil samples from 118 boring locations. The samples were collected at 0.5-foot depth intervals to various depths based on analytical results and depth to groundwater. The soil samples were submitted to a fixed-based laboratory for analysis by United States Environmental Protection Agency Method 8082A for total PCBs. Several soil samples had PCB concentrations above the State of Florida Direct Exposure Residential and Industrial Soil Cleanup Target Levels (SCTLs), and two soil samples had PCB concentrations above the State of Florida Leachability for Groundwater. No soil borings were located beneath impervious surfaces, such as concrete or asphalt. The current Land Use Control Implementation Plan (LUCIP) covers PCBs in soils above the residential SCTL, which includes the paved areas.

soil assessment↗

Temporal Stability of Surface Roughness Effects on Radar Based Soil Moisture Retrieval During the Corn Growth Cycle

A representative soil surface roughness parameterization needed for the retrieval of soil moisture from active microwave satellite observation is difficult to obtain through either in-situ measurements or remote sensing-based inversion techniques. Typically, for the retrieval of soil moisture, temporal variations in surface roughness are assumed to be negligible. Although previous investigations have suggested that this assumption might be reasonable for natural vegetation covers (Moran et al. 2002, Thoma et al. 2006), insitu measurements over plowed agricultural fields (Callens et al. 2006) have shown that the soil surface roughness can change considerably over time. This paper reports on the temporal stability of surface roughness effects on radar observations and soil moisture retrieved from these radar observations collected once a week during a corn growth cycle (May 10th - October 2002). The data set employed was collected during the Optimizing Production Inputs for Economic and Environmental Enhancement (OPE3) field campaign covering this 2002 corn growth cycle and consists of dual-polarized (HH and VV) L-band (1.6 GHz) acquired at view angles of 15, 35, and 55 degrees. Cross-polarized L baud radar data were also collected as part of this experiment, but are not used in the analysis reported on here. After accounting for vegetation effects on radar observations, time-invariant optimum roughness parameters were determined using the Integral Equation Method (IEM) and radar observations acquired over bare soil and cropped conditions (the complete radar data set includes entire corn growth cycle). The optimum roughness parameters, soil moisture retrieval uncertainty, temporal distribution of retrieval errors and its relationship with the weather conditions (e.g. rainfall and wind speed) have been analyzed. It is shown that over the corn growth cycle, temporal roughness variations due to weathering by rain are responsible for almost 50% of soil moisture retrieval uncertainty depending on the sensing configuration. The effects of surface roughness variations are found to be smallest for observations acquired at a view angle of 55 degrees and HH polarization. A possible explanation for this result is that at 55 degrees and HH polarization the effect of vertical surface height changes on the observed radar response are limited because the microwaves travel parallel to the incident plane and as a result will not interact directly with vertically oriented soil structures.

Joseph, A.T.↗

A tale of two extremes: Temperature sensitivity of carbon loss from cool and hot soils

Soils represent the largest terrestrial carbon (C) pool, and the flux of carbon dioxide (CO 2 ) from soils to the atmosphere is ~ 6-10 times more than anthropogenic emissions. Understanding responses of soil CO 2 emissions to warming is crucial for evaluating feedback to ongoing environmental changes. The relationship between microbial respiration and temperature is typically modeled using a Q 10 function. Generally, observations of the apparent Q 10 of soil respiration are higher for cold vs. warm ecosystems, reflecting expected biophysical controls of Arrhenius kinetics. However, results from two field warming experiments in the tropics contradict this expectation, both observing extraordinarily high soil respiration responses to in situ warming. Our overall objective for the proposed work is to reduce uncertainty in temperature sensitivity of soil C loss by systematically synthesizing underlying mechanisms related to soil C turnover and stabilization. We are evaluating the temperature sensitivity of soil respiration in ecosystems across temperature extremes (e.g., arctic/boreal and tropical systems) by integrating data collected from field warming experiments with machine learning and biogeochemical models.

54 ENVIRONMENTAL SCIENCES↗

A summary of microwave remote sensing investigations planned for BOREAS

The Boreal Ecosystem - Atmosphere Study (BOREAS) is a multidisciplinary field and remote sensing study that will be implemented jointly by the United States and Canada. The goal of BOREAS is to obtain an improved understanding of the interactions between the boreal forest biome and the atmosphere in order to clarify their roles in global change. Specific objectives are to improve the understanding of the processes that govern the exchanges of water, energy, heat, carbon, and trace gases between boreal ecosystems and the atmosphere, and to develop and validate remote sensing algorithms for transferring the understanding of these processes from local to regional scales. Two principal field sites, both within Canada, were selected. The northern site is located near Thompson, Manitoba, and the southern site encompasses Prince Albert National Park in Saskatchewan. The growing season in the northern site tends to be limited by growing-degree days while the southern site is limited by soil moisture and fire frequency. Most of the field work will occur at these two sites during 1993 and 1994 as part of six field campaigns. The first of these campaigns is scheduled for August 1993 and will involve instrument installation and an operational shakedown. Three large scale Intensive Field Campaigns (IFC's) are scheduled for 1994, along with two smaller scale Focused Field Campaigns (FFC's). The first 1994 campaign will be an FFC designed to capture the biome under completely frozen conditions during the winter. The second FFC and the first IFC are scheduled to capture the spring thaw period. Another IFC will take place in the summer during a period of maximum water stress. Finally, the third FFC will be scheduled to capture the collapse into senescence during the fall.

Mcdonald, Kyle C.↗

Precipitation and nitrogen application stimulate soil nitrous oxide emission

Precipitation and nitrogen (N) fertilization are the two most important drivers for soil nitrous oxide (N 2 O) emission. However, the effects of changes in N fertilization and precipitation patterns (i.e., precipitation intensity and frequency) on N 2 O emissions in agricultural fields are still unclear. In this study, we simulated soil N 2 O emission under different precipitation patterns (6 precipitation intensities, and 12 precipitation frequencies by either merging or splitting precipitation events) and N fertilization rates (low, typical, and high N fertilization) in a cornfield using the DeNitrification-DeComposition model. The model was parameterized and validated using meteorological data and N experimental measurements in Nashville, Tennessee, USA. Results showed that soil water filled pore space (WFPS) and simulated soil N 2 O emission increased as precipitation intensity increased. Less frequent but high intensity precipitation treatments reduced the soil WFPS by 25.2% and stimulated soil N 2 O emission by 45.3%, while more frequent but low intensity precipitation treatments increased soil WFPS by 9.0% and reduced soil N 2 O emission by 23.9%. Compared to typical N fertilization, the sensitivity of soil N 2 O emission to precipitation was higher under high N than low N fertilization treatments, and the response ratios were 50.0% and 40.1%, respectively. There was significant interactive effect of precipitation intensity and N fertilization on soil N 2 O emission. Furthermore, these findings improved our understanding of precipitation and N impacts on soil N 2 O emissions and provided useful knowledge for irrigation and N fertilizer management in agriculture to mitigate greenhouse gas emissions.

54 ENVIRONMENTAL SCIENCES↗

Calibration procedures for measurement of reflectance factor in remote sensing field research

A means by which measurements of the optical properties of crops and soils can be knowledgeably compared from site to site and instrument to instrument is presented in detail. The definition of bidirectional reflectance factor is reviewed and discussed. Procedures for field implementation are illustrated and discussed. Spectral and goniometric properties of laboratory and field reference surfaces are given. It is concluded that intelligent use of the bidirectional reflectance factor technique is an accurate and practical means to obtain the spectral, optical properties of crops and soils needed for advancements in agricultural remote sensing.

Robinson, B. F.↗

Coupled Long-Term Experiment and Model Investigation of the Differential Response of Plants and Soil Microbes in a Changing Permafrost Tundra Ecosystem

The major research goal of this project was to understand and quantify the fate of carbon stored in permafrost ecosystems using a combination of field and laboratory experiments to measure and model isotope ratios and carbon fluxes in a tundra ecosystem exposed to experimental warming. Field measurements centered on a two-factor experimental warming to increase air and soil temperatures alone, and in combination, at a tundra field site in Alaska. A second manipulation of water table was embedded within the warming treatment such that both major environmental factors (temperature, moisture) controlling ecosystem carbon dynamics were experimentally altered. The experiment was interfaced with modeling activities using both data assimilation and forward modeling approaches. Models were used to make forecasts of ecosystem carbon dynamics beyond the time frame and environmental space of the experiment itself. This project was a follow-on to previous work that made use of the same field manipulation; here we continued the experimental manipulation in order to test new hypotheses regarding long-term effects of warming and permafrost thaw and subsequent changes in moisture availability on ecosystem carbon dynamics. These results were linked with ongoing synthesis and model intercomparison activities through the Permafrost Carbon Network, which led to additional synthesis publications as a result of collaboration and data sharing from this project. As key outcomes, this project has produced new papers published in the peer-reviewed literature, archived datasets used for model-data intercomparisons, trained graduate students and postdoctoral researchers, and has raised awareness about the vulnerability of permafrost carbon to a wider audience.

54 ENVIRONMENTAL SCIENCES↗

Stronger warming effects on microbial abundances in colder regions

Soil microbes play critical roles in regulating terrestrial carbon (C) cycle and its feedback to climate change. However, it is still unclear how the soil microbial community and abundance respond to future climate change scenarios. In this meta-analysis, we synthesized the responses of microbial community and abundance to experimental warming from 64 published field studies. Our results showed that warming significantly increased soil microbial abundance by 7.6% on average. When grouped by vegetation or soil types, tundras and histosols had the strongest microbial responses to warming with increased microbial, fungal, and bacterial abundances by 15.0%, 9.5% and 37.0% in tundra, and 16.5%, 13.2% and 13.3% in histosols, respectively. We found significant negative relationships of the response ratios of microbial, fungal and bacterial abundances with the mean annual temperature, indicating that warming had stronger effects in colder than warmer regions. Moreover, the response ratios of microbial abundance to warming were positively correlated with those of soil respiration. Our results therefore indicate that the large quantities of C stored in colder regions are likely to be more vulnerable to climate warming than the soil C stored in other warmer regions.

54 ENVIRONMENTAL SCIENCES↗