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At least 757 records · Page 42

A Genomic Catalog of Stress Response Genes in Anaerobic Fungi for Applications in Bioproduction

Anaerobic fungi are a potential biotechnology platform to produce biomass-degrading enzymes. Unlike model fungi such as yeasts, stress responses that are relevant during bioprocessing have not yet been established for anaerobic fungi. In this work, we characterize both the heat shock and unfolded protein responses of four strains of anaerobic fungi ( Anaeromyces robustus, Caecomyces churrovis, Neocallimastix californiae , and Piromyces finnis ). The inositol-requiring 1 (Ire1) stress sensor, which typically initiates the fungal UPR, was conserved in all four genomes. However, these genomes also encode putative transmembrane kinases with catalytic domains that are similar to the metazoan stress-sensing enzyme PKR-like endoplasmic reticulum kinase (PERK), although whether they function in the UPR of anaerobic fungi remains unclear. Furthermore, we characterized the global transcriptional responses of Anaeromyces robustus and Neocallimastix californiae to a transient heat shock. Both fungi exhibited the hallmarks of ER stress, including upregulation of genes with functions in protein folding, ER-associated degradation, and intracellular protein trafficking. Relative to other fungi, the genomes of Neocallimastigomycetes contained the greatest gene percentage of HSP20 and HSP70 chaperones, which may serve to stabilize their asparagine-rich genomes. Taken together, these results delineate the unique stress response of anaerobic fungi, which is an important step toward their development as a biotechnology platform to produce enzymes and valuable biomolecules.

Swift, Candice L.↗

Winds in late-type stars - Mechanisms of mass outflow

The four basic mechanisms that have been proposed for explaining the acceleration of winds in late-type stars are thermal pressure gradients, radiation pressure on circumstellar dust grains, momentum addition by Alfven waves, and momentum addition by periodic shock waves. Recent work in applying these mechanisms to stars is reviewed, with consideration given to whether these mechanisms can work, even in principle, and whether they are consistent with recent ultraviolet and X-ray data from the IUE and Einstein spacecraft. It is noted that thermally driven winds are likely important for late-type dwarfs, where the mass loss rates are small, and perhaps also in G giants and supergiants, but they cannot operate alone in the K and M giants and supergiants. It is thought that radiatively driven winds are probably unimportant for all cool stars, even the M supergiants with dusty circumstellar envelopes. In principle, Alfven waves can accelerate winds to high speeds so long as the field lines are initially open or forced open by some mechanism, but detailed calculations are needed. It is noted that, for the Miras and semiregular variable supergiants, periodic shock waves provide a simple way of producing rapid mass loss.

Linsky, J. L.↗

Formation of a spherical plasma liner for plasma-jet-driven magneto-inertial fusion

Plasma-jet-driven magneto-inertial fusion is an alternative approach to controlled nuclear fusion, which aims to utilize a line-replaceable dense plasma liner as a repetitive spherical compression driver. In this experiment, first measurements of the formation of a spherical argon plasma liner formed from 36 discrete pulsed plasma jets are obtained on the Plasma Liner Experiment. Properties including liner uniformity and morphology, plasma density, temperature, and ram pressure are assessed as a function of time throughout the implosion process and indicate an apparent transition from initial kinetic inter-jet interpenetration to collisional regime near stagnation times, in accordance with theoretical expectation. A lack of primary shock structures between adjacent jets during flight implies that arbitrarily smooth liners may be formed by way of corresponding improvements in jet parameters and control. The measurements facilitate the benchmarking of computational models and understanding the scaling of plasma liners toward fusion-relevant energy density.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Stellar Mass Black Hole Formation and Multimessenger Signals from Three-dimensional Rotating Core-collapse Supernova Simulations

We present self-consistent 3D core-collapse supernova simulations of a 40 ${M}_{\odot }$ progenitor model using the isotropic diffusion source approximation for neutrino transport and an effective general relativistic potential up to `0.9 s postbounce. We consider three different rotational speeds with initial angular velocities of ${{\rm{\Omega }}}_{0}=0$, 0.5, and 1 rad s -1 and investigate the impact of rotation on shock dynamics, black hole (BH) formation, and gravitational wave (GW) signals. The rapidly rotating model undergoes an early explosion at ~250 ms postbounce and shows signs of the low $T/| W| $ instability. We do not find BH formation in this model within ~460 ms postbounce. In contrast, we find BH formation at 776 ms postbounce and 936 ms postbounce for the nonrotating and slowly rotating models, respectively. The slowly rotating model explodes at ~650 ms postbounce, and the subsequent fallback accretion onto the proto–neutron star (PNS) results in BH formation. In addition, the standing accretion shock instability induces rotation of the PNS in the model that started with a nonrotating progenitor. Assuming conservation of specific angular momentum during BH formation, this corresponds to a BH spin parameter of a = J/M = 0.046. However, if no explosion sets in, all the angular momentum will eventually be accreted by the BH, resulting in a nonspinning BH. The successful explosion of the slowly rotating model drastically slows down the accretion onto the PNS, allowing continued cooling and contraction that results in an extremely high GW frequency (f ~ 3000 Hz) at BH formation, while the nonrotating model generates GW signals similar to our corresponding 2D simulations.

79 ASTRONOMY AND ASTROPHYSICS↗

Detection of X-ray emission from the remnant of the supernova 1006 A.D.

Results are reported for OSO-7 observations which positively identify the supernova remnant SN 1006 as a weak source of X-ray emission. Acceptable fits to the spectrum are obtained for both a power law with an energy index of about -2.3 and for thermal bremsstrahlung at a temperature corresponding to about 4 keV. The X-ray intensity over the range from 1 to 10 keV is found to be about 9 by 10 to the -11th power erg/sq cm per sec, and it is assumed that the X-rays originate in a plasma shell 4.4 pc in radius and heated by an expanding blast wave. The distance of SN 1006 is estimated as 1.2 kpc, its X-ray luminosity (1-10 keV) as about 1.6 by 10 to the 34th power erg/sec, and its initial kinetic energy as of the order of 10 to the 50th power ergs. It is noted that the reverse-shock model of X-ray production is also consistent with the data and that SN 1006 is the only supernova remnant for which both X-ray and radio emission have been detected, but no optical filaments have been observed.

Winkler, P. F., Jr.↗

Computation of supersonic turbulent flows over an inclined ogive-cylinder-flare

A supersonic turbulent flow over an ogive-cylinder-flare has been solved numerically. Initially, the parabolized Navier-Stokes equations are solved for the ogive cylinder back to a location upstream of the shock-wave and boundary-layer interaction. Then, the time-dependent Navier-Stokes equations with a thin-layer approximation are solved for the remaining cylinder-flare portion. Results for a Mach number of 2.9 and a unit Reynolds number of 11.42 x 10 to the 6th/m are obtained for angles of attack alpha = 0, 4, and 8 deg. Good agreement has been found between computed and experimental results of the surface pressure on the ogive-cylinder portion, and for the interaction region at alpha = 0 and 4 deg. The role of circumferential communication in a three-dimensional shock-wave and boundary-layer interaction flow field is discussed.

Hung, C. M.↗

Application of a full potential method for predicting supersonic flow fields and aerodynamic characteristics

A nonlinear aerodynamic prediction technique which solves the conservative full potential equation has been applied to the analysis of three waverider configurations. This technique was selected based on its capability to analyze the off-design characteristics of the waveriders. Very good correlations were achieved with surface pressure data for both the Mach 4 elliptic cone waverider and the Mach 6 caret-wing derivative. Off-design Mach number and angle-of-attack pressure correlations were very good for the elliptic cone waverider. The range of correlation with data exceeded that expected based on the theory limitations. A surface pressure integration routine was demonstrated and agreement between predicted aerodynamic forces and experimental force data for the Mach 4 waverider was excellent. Analysis of a nonconical waverider configuration was initiated where a discrete input option is used to achieve the computational gridding. Preliminary analysis of this configuration indicates the correct shock location will be predicted.

Jones, K. M.↗

A convergent series expansion for hyperbolic systems of conservation laws

The discontinuities piecewise analytic initial value problem for a wide class of conservation laws is considered which includes the full three-dimensional Euler equations. The initial interaction at an arbitrary curved surface is resolved in time by a convergent series. Among other features the solution exhibits shock, contact, and expansion waves as well as sound waves propagating on characteristic surfaces. The expansion waves correspond to he one-dimensional rarefactions but have a more complicated structure. The sound waves are generated in place of zero strength shocks, and they are caused by mismatches in derivatives.

Harabetian, E.↗

Acoustic waves in the solar atmosphere. VII - Non-grey, non-LTE H(-) models

The propagation and shock formation of radiatively damped acoustic waves in the solar chromosphere are studied under the assumption that H(-) is the only absorber; the opacity is non-grey. Deviations from local thermodynamic equilibrium (LTE) are permitted. The results of numerical simulations show the depth dependence of the heating by the acoustic waves to be insensitive to the mean state of the atmosphere. After the waves have developed into shocks, their energy flux decays exponentially with a constant damping length of about 1.4 times the pressure scale height, independent of initial flux and wave period. Departures from LTE have a strong influence on the mean temperature structure in dynamical chromosphere models; this is even more pronounced in models with reduced particle density - simulating conditions in magnetic flux tubes - which show significantly increased temperatures in response to mechanical heating. When the energy dissipation of the waves is sufficiently large to dissociate most of the H(-) ions, a strong temperature rise is found that is reminiscent of the temperature structure in the transition zone between chromosphere and corona; the energy flux remaining in the waves then drives mass motions.

Schmitz, F.↗

Conditions for the formation of massive stars

Upper limits on the masses of stars that can form are reexamined and models for the inflow of matter through cocoons around stars of 60, 100, and 200 solar masses are calculated. Consideration is given to the general conditions that must hold at the inner and outer boundaries of a protostellar cocoon; limits on the dust-to-gas ratios and mass inflow rates that will permit inflow onto very massive stars are determined. It is found that inflow can occur if intermediate-sized grains (0.05-0.25 micron) are missing from the initial gas/dust mixture. The existence of massive stars in certain locations in galaxies indicates that preconditioning of the interstellar medium by shocks or turbulence is necessary for massive star formation.

Wolfire, Mark G.↗

IUE investigations of SN 1987A

IUE observations of the SN 1987A began shortly after the discovery and have been frequent through 1988 and 1989, using the fine error sensor for photometry, low dispersion spectra for the supernova spectrum, and high dispersion observations for the interstellar medium when the supernova was bright and for circumstellar gas surrounding the supernova as the initial event faded. The UV data were very useful in determining which star exploded, assessing the ionizing pulse produced as the shock hit the surface of the star, and in constraining the stellar evolution that preceded the explosion through observations of a circumstellar shell.

Kirshner, Robert P.↗

Observing SN 1987A with the International Ultraviolet Explorer

The International Ultraviolet Explorer (IUE) satellite played a leading role in elucidating the nature of SN 1987A, providing a unique ultraviolet perspective on the brightest supernova since 1604. IUE observations of SN 1987A began promptly after discovery and were frequent through 1988 and 1989, using the FES (Fine Error Sensor) for photometry, low dispersion spectra for the supernova spectrum, high dispersion observations for the interstellar medium when the supernova was bright, and for circumstellar gas surrounding the supernova as the initial event faded. The UV data were especially useful in determining which star exploded, assessing the ionizing pulse produced as the shock hit the surface of the star, and in constraining the stellar evolution that preceded the explosion through observation of a circumstellar shell.

Kirshner, Robert P.↗

Two-stream modeling of plasmaspheric refilling

Plasmaspheric refilling on an L = 4 flux tube was studied by using a time-dependent, hydrodynamic plasmaspheric flow model in which the ion streams from the two hemispheres are treated as distinct fluids. In the model the continuity, momentum, and energy equations of a two-ion (O(+) and H(+)), quasi-neutral, currentless plasma are solved along a closed geomagnetic field line; diffusive equilibrium is not assumed. collisions between all stream pairs and with neutral species are included. The model includes a corotating, tilted dipole magnetic field and neutral winds. Ionospheric sources and sinks are accounted for in a self-consistent manner. Electrons are assumed to be heated by photoelectrons. The model flux tube extends from a 200-km altitude in one hemisphere to a 200-km altitude in the other hemisphere. Initially, the upwelling streams pass through each other practically unimpeded. When the streams approach the boundary in the conjugate ionosphere, a shock develops there, which moves upward and dissipates slowly; at about the same time a reverse shock develops in the hemisphere of origin, which moves upward. After about 1 hour, large shocks develop in each stream near the equator; these shocks move toward the equator and downward after crossing the equator. However, these shocks are probably artificial, because counterstreaming flows occur in each H(+) fluid, which the model can only handle by creating shocks.

Guiter, S. M.↗

Computation of Supersonic Turbulent Flows over an Inclined Ogive-Cylinder-Flare

A supersonic turbulent flow over an ogive-cylinder-flare has been solved numerically. The calculations proceed in two parts. Initially, the parabolized Navier-Stokes equations are solved for the ogive cylinder back to a location upstream of the shock-wave and boundary-layer interaction. Then, the time-dependent Navier-Stokes equations with a thin-layer approximation are solved for the remaining cylinder-flare portion. Results for a Mach number of 2.0 and a unit Reynolds number of 11.42 x 10(exp 6)/m are obtained for angles of attack alpha = 0, 4, and 8 deg. Good agreement has been found between computed and experimental results of the surface pressure on the ogive-cylinder portion and for the interaction region at alpha = 0 and 4 deg. The role of circumferential communication in a three-dimensional shock-wave and boundary-layer interaction flowfield is discussed.

Hung, C. M.↗

Recent Enhancements to USM3D Unstructured Flow Solver for Unsteady Flows

The NASA USM3D unstructured flow solver is undergoing extensions to address dynamic flow problems in support of NASA and NAVAIR efforts to study the applicability of Computational Fluid Dynamics tools for the prediction of aircraft stability and control characteristics. The initial extensions reported herein include two second-order time stepping schemes, Detached-Eddy Simulation, and grid motion. This paper reports the initial code verification and validation assessment of the dynamic flow capabilities of USM3D. The cases considered are the classic inviscid shock-tube problem, low Reynolds number wake shedding from a NACA 0012 airfoil, high Reynolds number DES-based wake shedding from a 4-to-1 length-to-diameter cylinder, and forced pitch oscillation of a NACA 0012 airfoil with inviscid and turbulent flow.

Pandya, Mohagna J.↗

Simulations and models for the Richtmyer–Meshkov instability with broadband perturbations

The Richtmyer–Meshkov instability (RMI) is shock driven and affects many phenomena from inertial fusion to supernova explosions. The behavior of single-modes in the RMI has been studied extensively but less is known with the broadband perturbations that occur in applications. Here, we describe extensive numerical simulations and modeling of the RMI with broadband perturbations with an initial power spectrum of the form P ∝ km, where k is the wavenumber. The hydrodynamic condition is the same as in the θ-Group Collaboration [Thornber et al., “Late-time growth rate, mixing, and anisotropy in the multimode narrowband Richtmyer–Meshkov instability: The θ-group collaboration,” Phys. Fluids 29, 105107 (2017)] with a Mach ∼1.86 shock and gamma-law = 5/3 fluids with Atwood number A = −0.5. The bubble amplitude hB is found to grow in two stages. Initially, hB undergoes a phase-inversion and grows linearly in time (t) at a rate consistent with a linear theory. Asymptotically, hB grows as a power law ∼tθ when k|hB| > O(1), where k is the average wavenumber for the initial spectrum. The RMI behavior in simulations and modeling agree over a wide range of exponent m, spectral width, initial amplitude, and time. The agreement is quantified objectively using statistical analysis.

79 ASTRONOMY AND ASTROPHYSICS↗

Simulations and models for the Richtmyer– Meshkov instability with broadband perturbations

The Richtmyer–Meshkov instability (RMI) is shock driven and affects many phenomena from inertial fusion to supernova explosions. The behavior of single-modes in the RMI has been studied extensively but less is known with the broadband perturbations that occur in applications. Here, we describe extensive numerical simulations and modeling of the RMI with broadband perturbations with an initial power spectrum of the form P∝ k m , where k is the wavenumber. The hydrodynamic condition is the same as in the $\theta$-Group Collaboration [Thornber et al., “Late-time growth rate, mixing, and anisotropy in the multimode narrowband Richtmyer–Meshkov instability: The $\theta$-group collaboration,” Phys. Fluids 29, 105107 (2017)] with a Mach ~1.86 shock and gamma-law = 5/3 fluids with Atwood number A = 0.5. The bubble amplitude h B is found to grow in two stages. Initially, h B undergoes a phase-inversion and grows linearly in time (t) at a rate consistent with a linear theory. Asymptotically, h B grows as a power law $~t^{\theta}$ when $\langle{k}\rangle{|h_B|}$ > O(1), where $\langle{k}\rangle$ is the average wavenumber for the initial spectrum. The RMI behavior in simulations and modeling agree over a wide range of exponent m, spectral width, initial amplitude, and time. The agreement is quantified objectively using statistical analysis.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗