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At least 757 records · Page 42

The impact of long-range dispersal on gene surfing

Range expansions lead to distinctive patterns of genetic variation in populations, even in the absence of selection. These patterns and their genetic consequences have been well studied for populations advancing through successive short-ranged migration events. However, most populations harbor some degree of long-range dispersal, experiencing rare yet consequential migration events over arbitrarily long distances. Although dispersal is known to strongly affect spatial genetic structure during range expansions, the resulting patterns and their impact on neutral diversity remain poorly understood. Here, we systematically study the consequences of long-range dispersal on patterns of neutral variation during range expansion in a class of dispersal models which spans the extremes of local (effectively short-ranged) and global (effectively well-mixed) migration. We find that sufficiently long-ranged dispersal leaves behind a mosaic of monoallelic patches, whose number and size are highly sensitive to the distribution of dispersal distances. We develop a coarse-grained model which connects statistical features of these spatial patterns to the evolution of neutral diversity during the range expansion. We show that growth mechanisms that appear qualitatively similar can engender vastly different outcomes for diversity: Depending on the tail of the dispersal distance distribution, diversity can be either preserved (i.e., many variants survive) or lost (i.e., one variant dominates) at long times. Our results highlight the impact of spatial and migratory structure on genetic variation during processes as varied as range expansions, species invasions, epidemics, and the spread of beneficial mutations in established populations.

59 BASIC BIOLOGICAL SCIENCES↗

Structure and dynamics of SARS-CoV-2 proofreading exoribonuclease ExoN

High-fidelity replication of the large RNA genome of coronaviruses (CoVs) is mediated by a 3'-to-5' exoribonuclease (ExoN) in nonstructural protein 14 (nsp14), which excises nucleotides including antiviral drugs misincorporated by the low-fidelity viral RNA-dependent RNA polymerase (RdRp) and has also been implicated in viral RNA recombination and resistance to innate immunity. Here, we determined a 1.6-Å resolution crystal structure of severe acute respiratory syndrome CoV 2 (SARS-CoV-2) ExoN in complex with its essential cofactor, nsp10. The structure shows a highly basic and concave surface flanking the active site, comprising several Lys residues of nsp14 and the N-terminal amino group of nsp10. Modeling suggests that this basic patch binds to the template strand of double-stranded RNA substrates to position the 3' end of the nascent strand in the ExoN active site, which is corroborated by mutational and computational analyses. We also show that the ExoN activity can rescue a stalled RNA primer poisoned with sofosbuvir and allow RdRp to continue its extension in the presence of the chain-terminating drug, biochemically recapitulating proofreading in SARS-CoV-2 replication. Molecular dynamics simulations further show remarkable flexibility of multidomain nsp14 and suggest that nsp10 stabilizes ExoN for substrate RNA binding to support its exonuclease activity. Our high-resolution structure of the SARS-CoV-2 ExoN–nsp10 complex serves as a platform for future development of anticoronaviral drugs or strategies to attenuate the viral virulence.

60 APPLIED LIFE SCIENCES↗

Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex

Previously, we showed that the nuclear import receptor Importin-9 wraps around the H2A-H2B core to chaperone and transport it from the cytoplasm to the nucleus. However, unlike most nuclear import systems where RanGTP dissociates cargoes from their importins, RanGTP binds stably to the Importin-9•H2A-H2B complex, and formation of the ternary RanGTP•Importin-9•H2A-H2B complex facilitates H2A-H2B release to the assembling nucleosome. It was unclear how RanGTP and the cargo H2A-H2B can bind simultaneously to an importin, and how interactions of the three components position H2A-H2B for release. Here, we show cryo-EM structures of Importin-9•RanGTP and of its yeast homolog Kap114, including Kap114•RanGTP, Kap114•H2A-H2B, and RanGTP•Kap114•H2A-H2B, to explain how the conserved Kap114 binds H2A-H2B and RanGTP simultaneously and how the GTPase primes histone transfer to the nucleosome. In the ternary complex, RanGTP binds to the N-terminal repeats of Kap114 in the same manner as in the Kap114/Importin-9•RanGTP complex, and H2A-H2B binds via its acidic patch to the Kap114 C-terminal repeats much like in the Kap114/Importin-9•H2A-H2B complex. Ran binds to a different conformation of Kap114 in the ternary RanGTP•Kap114•H2A-H2B complex. Here, Kap114 no longer contacts the H2A-H2B surface proximal to the H2A docking domain that drives nucleosome assembly, positioning it for transfer to the assembling nucleosome or to dedicated H2A-H2B chaperones in the nucleus.

59 BASIC BIOLOGICAL SCIENCES↗

Landscape fragmentation overturns classical metapopulation thinking

Habitat loss and isolation caused by landscape fragmentation represent a growing threat to global biodiversity. Existing theory suggests that the process will lead to a decline in metapopulation viability. However, since most metapopulation models are restricted to simple networks of discrete habitat patches, the effects of real landscape fragmentation, particularly in stochastic environments, are not well understood. To close this major gap in ecological theory, we developed a spatially explicit, individual-based model applicable to realistic landscape structures, bridging metapopulation ecology and landscape ecology. This model reproduced classical metapopulation dynamics under conventional model assumptions, but on fragmented landscapes, it uncovered general dynamics that are in stark contradiction to the prevailing views in the ecological and conservation literature. Notably, fragmentation can give rise to a series of dualities: a) positive and negative responses to environmental noise, b) relative slowdown and acceleration in density decline, and c) synchronization and desynchronization of local population dynamics. Furthermore, counter to common intuition, species that interact locally (“residents”) were often more resilient to fragmentation than long-ranging “migrants.” This set of findings signals a need to fundamentally reconsider our approach to ecosystem management in a noisy and fragmented world.

54 ENVIRONMENTAL SCIENCES↗

Inhibitory Mechanism of an Allosteric Antibody Targeting the Glucagon Receptor

Elevated glucagon levels and increased hepatic glucagon receptor (GCGR) signaling contribute to hyperglycemia in type 2 diabetes. We have identified a monoclonal antibody that inhibits GCGR, a class B G-protein coupled receptor (GPCR), through a unique allosteric mechanism. Receptor inhibition is mediated by the binding of this antibody to two distinct sites that lie outside of the glucagon binding cleft. One site consists of a patch of residues that are surface-exposed on the face of the extracellular domain (ECD) opposite the ligand-binding cleft, whereas the second binding site consists of residues in the A helix of the ECD. A docking model suggests that the antibody does not occlude the ligand-binding cleft. We solved the crystal structure of GCGR ECD containing a naturally occurring G40S mutation and found a shift in the register of the A helix that prevents antibody binding. We also found that alterations in the A helix impact the normal function of GCGR. We present a model for the allosteric inhibition of GCGR by a monoclonal antibody that may form the basis for the development of allosteric modulators for the treatment of diabetes and other class B GPCR-related diseases.

59 BASIC BIOLOGICAL SCIENCES↗

The Physics Imposed on a Streaming Operator by Spherical Transport Problems

The streaming operator, which generates a displacement of a particle on a straight line at a constant speed in transport theory, is derived algebraically from a spherical coordinate formulation of Newton’s second law. This derivation leads to an operator that has more partial derivatives than a Cartesian coordinate formulation of the operator. The additional partial derivatives, which are with respect to the normalized velocity variables of a particle, take into account the intrinsic curvature of a ball. Moreover, these partial derivatives mitigate ray effects, which arise when a finite number of normalized velocities (also called directions or discrete ordinates) are used to simulate a continuous S 2 sphere of directions, by rotating the polar axis of the S 2 sphere into the radial direction of the coordinate system. As a consequence of this rotation, the number of actual discrete ordinates is greatly amplified to an enormous number of effective discrete ordinates by a multiplier that is equal to the number of patches that partitions a spherical surface. In addition to the derivation of the streaming operator, we provide in closed form a solution to the system of characteristic equations that is equivalent to the streaming operator. Furthermore, the solution to the system of characteristic equations enables the construction of an integral operator that is the inverse to the streaming operator. Examples in which ray effects are immensely mitigated by spherical coordinates are presented.

integral operator↗

Mapping Lake Sturgeon Spawning Habitat in the Upper Tennessee River using Side-Scan Sonar

Abstract The Lake Sturgeon Acipenser fulvescens is a fish species that was once dispersed widely throughout the Mississippi River drainage but was largely extirpated from the southern portions of its range by overfishing and habitat degradation. There is an ongoing restoration effort to reestablish the Lake Sturgeon to rivers of the southeastern United States. Reintroduced juvenile Lake Sturgeon now occupy several reservoirs separated from each other by hydroelectric dams along the upper Tennessee River. To complete their life history, Lake Sturgeon will migrate upriver from reservoir habitats to more lotic habitats and spawn over coarse rocky substrate, even in the tailwaters of impassable dams. Using low-cost, consumer-grade, side-scan sonar and a GIS, we mapped the substrate of four tailwaters that may be future spawning locations for Lake Sturgeon. We used video imagery collected from random locations within the mapped areas to validate our digitization of sonar imagery. We calculated the area of four substrate classes displayed in the maps to evaluate that aspect of the suitability of each of the tailwaters for Lake Sturgeon spawning. The revised maps showed that the best spawning substrate (unembedded, coarse, rocky substrate, 6–25 cm in diameter) comprised 17.0–30.5% of the total area mapped at each tailwater, while the least suitable substrate class (fine sediment, <0.2 cm in diameter) comprised 6.2–30.7% of the mapped areas. Our results suggest that any future spawning events by Lake Sturgeon below each of these dams are likely to encounter some suitable spawning substrate patches, while management opportunities exist to supplement tailwater areas with suitable spawning substrate. Received March 11, 2016; accepted May 30, 2016 Published online August 31, 2016

Walker, Daniel J.↗

Enhanced torsional actuation and stress coupling in Mn-modified 0.93(Na 0.5 Bi 0.5 TiO 3 )-0.07BaTiO 3 lead-free piezoceramic system

This paper is concerned with the development of a piezoelectric d 15 shear-induced torsion actuator made of a lead-free piezoceramic material exhibiting giant piezoelectric shear stress coefficient (e 15 ) and piezoelectric transverse shear actuation force comparable to that of leadbased shear-mode piezoceramics. The Mn-modified 0.93(Na 0.5 Bi 0.5 TiO 3 )-0.07BaTiO 3 (NBT-BTMn) composition exhibited excellent properties as a torsional transducer with piezoelectric shear stress coefficient on the order of 11.6 C m –2 . The torsional transducer, consisting of two oppositely polarized NBT-BT-Mn d 15 mode piezoceramic shear patches, provided a rate of twist of 0.08 mm m –1 V –1 under quasi-static 150 V drive. The high value of piezoelectric shear d 15 coefficient in NBT-BT-Mn sample further demonstrated its potential in practical applications. Lastly, these results confirm that the lead-free piezoceramics can be as effective as their lead-based counterparts.

36 MATERIALS SCIENCE↗

Permeant calcium ion feed-through regulation of single inositol 1,4,5-trisphosphate receptor channel gating

The ubiquitous inositol 1,4,5-trisphosphate (InsP 3 ) receptor (InsP 3 R) Ca 2+ release channel plays a central role in the generation and modulation of intracellular Ca 2+ signals, and is intricately regulated by multiple mechanisms including cytoplasmic ligand (InsP 3 , free Ca 2+ , free ATP 4- ) binding, posttranslational modifications, and interactions with cytoplasmic and endoplasmic reticulum (ER) luminal proteins. However, regulation of InsP 3 R channel activity by free Ca 2+ in the ER lumen ([Ca 2+ ] ER ) remains poorly understood because of limitations of Ca 2+ flux measurements and imaging techniques. Here, we used nuclear patch-clamp experiments in excised luminal-side-out configuration with perfusion solution exchange to study the effects of [Ca 2+ ] ER on homotetrameric rat type 3 InsP 3 R channel activity. In optimal [Ca 2+ ] i and subsaturating [InsP 3 ], jumps of [Ca 2+ ] ER from 70 nM to 300 µM reduced channel activity significantly. This inhibition was abrogated by saturating InsP 3 but restored when [Ca 2+ ] ER was raised to 1.1 mM. In suboptimal [Ca 2+ ] i , jumps of [Ca 2+ ] ER (70 nM to 300 µM) enhanced channel activity. Thus, [Ca 2+ ] ER effects on channel activity exhibited a biphasic dependence on [Ca 2+ ] i . In addition, the effect of high [Ca 2+ ] ER was attenuated when a voltage was applied to oppose Ca 2+ flux through the channel. These observations can be accounted for by Ca 2 + flux driven through the open InsP 3 R channel by [Ca 2+ ] ER , raising local [Ca 2+ ] i around the channel to regulate its activity through its cytoplasmic regulatory Ca 2+ -binding sites. Importantly, [Ca 2+ ] ER regulation of InsP 3 R channel activity depended on cytoplasmic Ca 2+ -buffering conditions: it was more pronounced when [Ca2+] i was weakly buffered but completely abolished in strong Ca 2+ -buffering conditions. With strong cytoplasmic buffering and Ca 2+ flux sufficiently reduced by applied voltage, both activation and inhibition of InsP 3 R channel gating by physiological levels of [Ca 2+ ] ER were completely abolished. Collectively, these results rule out Ca 2+ regulation of channel activity by direct binding to the luminal aspect of the channel.

59 BASIC BIOLOGICAL SCIENCES↗

Channel properties of the splicing isoforms of the olfactory calcium-activated chloride channel Anoctamin 2

Anoctamin (ANO)2 (or TMEM16B) forms a cell membrane Ca 2+ -activated Cl - channel that is present in cilia of olfactory receptor neurons, vomeronasal microvilli, and photoreceptor synaptic terminals. Alternative splicing of Ano2 transcripts generates multiple variants with the olfactory variants skipping exon 14 and having alternative splicing of exon 4. In the present study, 5' rapid amplification of cDNA ends analysis was conducted to characterize the 5'end of olfactory Ano2 transcripts, which showed that the most abundant Ano2 transcripts in the olfactory epithelium contain a novel starting exon that encodes a translation initiation site, whereas transcripts of the publically available sequence variant, which has an alternative and longer 5' end, were present in lower abundance. With two alternative starting exons and alternative splicing of exon 4, four olfactory ANO2 isoforms are thus possible. Patch-clamp experiments in transfected HEK293T cells expressing these isoforms showed that N-terminal sequences affect Ca 2+ sensitivity and that the exon 4–encoded sequence is required to form functional channels. Coexpression of the two predominant isoforms, one with and one without the exon 4 sequence, as well as coexpression of the two rarer isoforms showed alterations in channel properties, indicating that different isoforms interact with each other. Furthermore, channel properties observed from the coexpression of the predominant isoforms better recapitulated the native channel properties, suggesting that the native channel may be composed of two or more splicing isoforms acting as subunits that together shape the channel properties.

59 BASIC BIOLOGICAL SCIENCES↗

Regulation of NMDAR activation efficiency by environmental factors and subunit composition

NMDA receptors (NMDAR) convert the major excitatory neurotransmitter glutamate into a synaptic signal. A key question is how efficiently the ion channel opens in response to the rapid exposure to presynaptic glutamate release. Here, we applied glutamate to single channel outside-out patches and measured the successes of channel openings and the latency to first opening to assay the activation efficiency of NMDARs under different physiological conditions and with different human subunit compositions. For GluN1/GluN2A receptors, we find that various factors, including intracellular ATP and GTP, can enhance the efficiency of activation presumably via the intracellular C-terminal domain. Notably, an energy-based internal solution or increasing the time between applications to increase recovery time improved efficiency. However, even under these optimized conditions and with a 1-s glutamate application, there remained around 10–15% inefficiency. Channel activation became more inefficient with brief synaptic-like pulses of glutamate at 2 ms. Of the different NMDAR subunit compositions, GluN2B-containing NMDARs showed the lowest success rate and longest latency to first openings, highlighting that they display the most distinct activation mechanism. In contrast, putative triheteromeric GluN1/GluN2A/GluN2B receptors showed high activation efficiency. Despite the low open probability, NMDARs containing either GluN2C or GluN2D subunits displayed high activation efficiency, nearly comparable with that for GluN2A-containing receptors. These results highlight that activation efficiency in NMDARs can be regulated by environmental surroundings and varies across different subunits.

He, Miaomiao (ORCID:0000000203179136)↗

Influence of dual nitrogen and phosphorus additions on nutrient uptake and saturation kinetics in a forested headwater stream

Nitrogen (N) and phosphorus (P) can limit autotrophic and heterotrophic metabolism in lotic ecosystems, yet most studies that evaluate biotic responses to colimitation focus on patch-scale (e.g., nutrient diffusing substrata) rather than stream-scale responses. In this study, we evaluated the effects of single and dual N and P additions on ambient nutrient uptake rates and saturation kinetics during two biologically contrasting seasons (spring, autumn) in Walker Branch, a temperate forested headwater stream in Tennessee, USA. In each season, we used separate instantaneous pulse additions to quantify nutrient uptake rates and saturation kinetics of N (nitrate) and P (phosphate). We then used steady-state injections to elevate background stream water concentrations (to low and then high background concentrations) of one nutrient (e.g., N) and released instantaneous pulses of the other nutrient (e.g., P). We predicted that elevating the background concentration of one nutrient would result in a lower ambient uptake length and a higher maximum areal uptake rate of the other nutrient in this colimited stream. Our prediction held true in spring, as maximum areal uptake rate of N increased with elevated P concentrations from 185 µg m –2 min –1 (no added P) to 354 µg m –2 min –1 (high P). This pattern was not observed in autumn, as uptake rates of N were not measurable when P was elevated. Further, elevating background N concentration in either season did not significantly increase P uptake rates, likely because adsorption rather than biotic uptake dominated P dynamics. Laboratory P sorption assays demonstrated that Walker Branch sediments had a high adsorption capacity and were likely a sink for P during most pulse nutrient additions. Furthermore, it may be difficult to use coupled pulse nutrient additions to evaluate biotic uptake of N and P in streams with strong P adsorption potential. Future efforts should use dual nutrient addition techniques to investigate reach-scale coupled biogeochemical cycles (C–N–P, and other elemental cycles [e.g., Fe, Mo]) across seasons, biomes, and land-use types and over longer time periods.

54 ENVIRONMENTAL SCIENCES↗

A SURVEY OF RADIO RECOMBINATION LINES USING THE OOTY RADIO TELESCOPE AT 328 MHz IN THE INNER GALAXY

A survey of radio recombination lines in the Galactic plane with longitude -32 Degree-Sign < l < +80 Degree-Sign and latitude b < {+-}3 Degree-Sign using Ooty Radio Telescope (ORT) at 328 MHz is reported. ORT observations were made using a New Digital Backend (NDB) recently added to the telescope. With the NDB ORT had a beam of 2.{sup 0}3 Multiplication-Sign 2.{sup 0}2 sec({delta}) and a passband of {approx}1 MHz in the spectral line mode. The above-mentioned Galactic region was divided into {approx}2 Degree-Sign Multiplication-Sign 2 Degree-Sign patches with the ORT beam pointed to the center. The ORT observations form a study of the distribution of extended low-density warm-ionized medium (ELDWIM) in the inner Galaxy using H271{alpha} RLs. By obtaining kinematical distances using V{sub LSR} of the H271{alpha} RLs, the distribution of ELDWIM clouds within the inner Galaxy has been deduced for the region given above.

79 ASTRONOMY AND ASTROPHYSICS↗

THE FLARE-ONA OF EK DRACONIS

EK Draconis (HD 129333: G1.5 V) is a well-known young (50 Myr) solar analog. In 2012, Hubble Space Telescope returned to EK Dra to follow up a far-ultraviolet (FUV) SNAPshot visit by Cosmic Origins Spectrograph (COS) two years earlier. The brief SNAP pointing had found surprisingly redshifted, impulsively variable subcoronal “hot-line” emission of Si iv 1400 Å (T ∼ 8 × 10{sup 4} K). Serendipitously, the 2012 follow-on program witnessed one of the largest FUV flares ever recorded on a sunlike star, which again displayed strong redshifts (downflows) of 30–40 km s{sup −1}, even after compensating for small systematics in the COS velocity scales, uncovered through a cross-calibration by Space Telescope Imaging Spectrograph (STIS). The (now reduced, but still substantial) ∼10 km s{sup −1} hot-line redshifts outside the flaring interval did not vary with rotational phase, so cannot be caused by “Doppler imaging” (bright surface patches near a receding limb). Density diagnostic O iv] 1400 Å multiplet line ratios of EK Dra suggest n{sub e} ∼ 10{sup 11} cm{sup −3}, an order of magnitude larger than in low-activity solar twin α Centauri A, but typical of densities inferred in large stellar soft X-ray events. The self-similar FUV hot-line profiles between the flare decay and the subsequent more quiet periods, and the unchanging but high densities, reinforce a long-standing idea that the coronae of hyperactive dwarfs are flaring all the time, in a scale-free way; a flare-ona if you will. In this picture, the subsonic hot-line downflows probably are a byproduct of the post-flare cooling process, something like “coronal rain” on the Sun. All in all, the new STIS/COS program documents a complex, energetic, dynamic outer atmosphere of the young sunlike star.

79 ASTRONOMY AND ASTROPHYSICS↗

TWO TYPES OF MAGNETIC RECONNECTION IN CORONAL BRIGHT POINTS AND THE CORRESPONDING MAGNETIC CONFIGURATION

Coronal bright points (CBPs) are long-lived small-scale brightenings in the solar corona. They are generally explained by magnetic reconnection. However, the corresponding magnetic configurations are not well understood. We carry out a detailed multi-wavelength analysis of two neighboring CBPs on 2007 March 16, observed in soft X-ray (SXR) and EUV channels. It is seen that the SXR light curves present quasi-periodic flashes with an interval of {approx}1 hr superposed over the long-lived mild brightenings, suggesting that the SXR brightenings of this type of CBPs might consist of two components: one is the gentle brightenings and the other is the CBP flashes. It is found that the strong flashes of the bigger CBP are always accompanied by SXR jets. The potential field extrapolation indicates that both CBPs are covered by a dome-like separatrix surface, with a magnetic null point above. We propose that the repetitive CBP flashes, as well as the recurrent SXR jets, result from the impulsive null-point reconnection, while the long-lived brightenings are due to the interchange reconnection along the separatrix surface. Although the EUV images at high-temperature lines resemble the SXR appearance, the 171 Angstrom-Sign and 195 Angstrom-Sign channels reveal that the blurry CBP in SXR consists of a cusp-shaped loop and several separate bright patches, which are explained to be due to the null-point reconnection and the separatrix reconnection, respectively.

79 ASTRONOMY AND ASTROPHYSICS↗

THE EFFECT OF ABSORPTION SYSTEMS ON COSMIC REIONIZATION

We use large-scale simulations to investigate the morphology of reionization during the final, overlap phase. Our method uses an efficient three-dimensional smoothing technique that takes into account the finite mean free path due to absorption systems, {lambda}{sub abs}, by only smoothing over scales R{sub s} < {lambda}{sub abs}. The large dynamic range of our calculations is necessary to resolve the neutral patches left at the end of reionization within a representative volume; we find that simulation volumes exceeding several hundred Mpc on a side are necessary in order to properly model reionization when the neutral fraction is {approx_equal} 0.01-0.3. Our results indicate a strong dependence of percolation morphology on a large and uncertain region of model parameter space. The single most important parameter is the mean free path to absorption systems, which serve as opaque barriers to ionizing radiation. If these absorption systems were as abundant as some realistic estimates indicate, the spatial structure of the overlap phase is considerably more complex than previously predicted. In view of the lack of constraints on the mean free path at the highest redshifts, current theories that do not include absorption by Lyman-limit systems, and in particular three-dimensional simulations, may underestimate the abundance of neutral clouds at the end of reionization. This affects predictions for the 21 cm signal associated with reionization, interpretation of absorption features in quasar spectra at z {approx} 5-6, the connection between reionization and the local universe, and constraints on the patchiness and duration of reionization from temperature fluctuations measured in the cosmic microwave background arising from the kinetic Sunyaev-Zel'dovich effect.

79 ASTRONOMY AND ASTROPHYSICS↗

STUDY OF SINGLE-LOBED CIRCULAR POLARIZATION PROFILES IN THE QUIET SUN

The existence of asymmetries in the circular polarization (Stokes V) profiles emerging from the solar photosphere has been known since the 1970s. These profiles require the presence of a velocity gradient along the line of sight (LOS), possibly associated with gradients of magnetic field strength, inclination, and/or azimuth. We have focused our study on the Stokes V profiles showing extreme asymmetry in the form of only one lobe. Using Hinode spectropolarimetric measurements, we have performed a statistical study of the properties of these profiles in the quiet Sun. We show their spatial distribution, their main physical properties, how they are related with several physical observables, and their behavior with respect to their position on the solar disk. The single-lobed Stokes V profiles occupy roughly 2% of the solar surface. For the first time, we have observed their temporal evolution and have retrieved the physical conditions of the atmospheres from which they emerged using an inversion code implementing discontinuities of the atmospheric parameters along the LOS. In addition, we use synthetic Stokes profiles from three-dimensional magnetoconvection simulations to complement the results of the inversion. The main features of the synthetic single-lobed profiles are in general agreement with the observed ones, lending support to the magnetic and dynamic topologies inferred from the inversion. The combination of all these different analyses suggests that most of the single-lobed Stokes V profiles are signals coming from the magnetic flux emergence and/or submergence processes taking place in small patches in the photosphere of the quiet Sun.

79 ASTRONOMY AND ASTROPHYSICS↗

FLOCCULENT FLOWS IN THE CHROMOSPHERIC CANOPY OF A SUNSPOT

High-quality imaging spectroscopy in the H{alpha} line, obtained with the CRisp Imaging SpectroPolarimeter (CRISP) at the Swedish 1-m solar Telescope (SST) at La Palma and covering a small sunspot and its surroundings, is studied. They exhibit ubiquitous flows both along fibrils making up the chromospheric canopy away from the spot and in the superpenumbra. We term these flows 'flocculent' to describe their intermittent character, that is, morphologically reminiscent of coronal rain. The flocculent flows are investigated further in order to determine their dynamic and morphological properties. For the measurement of their characteristic velocities, accelerations, and sizes, we employ a new versatile analysis tool, the CRisp SPectral EXplorer (CRISPEX), which we describe in detail. Absolute velocities on the order of 7.2-82.4 km s{sup -1} are found, with an average value of 36.5 {+-} 5.9 km s{sup -1} and slightly higher typical velocities for features moving toward the sunspot than away. These velocities are much higher than those determined from the shift of the line core, which shows patches around the sunspot with velocity enhancements of up to 10-15 km s{sup -1} (both red- and blueshifted). Accelerations are determined for a subsample of features that show clear accelerating or decelerating behavior, yielding an average of 270 {+-} 63 m s{sup -2} and 149 {+-} 63 m s{sup -2} for the accelerating and decelerating features, respectively. Typical flocculent features measure 627 {+-} 44 km in length and 304 {+-} 30 km in width. On average, 68 features are detected per minute, with an average lifetime of 67.7 {+-} 8.8 s. The dynamics and phenomenology of the flocculent flows suggest they may be driven by a siphon flow, where the flocculence could arise from a density perturbation close to one of the footpoints or along the loop structure.

79 ASTRONOMY AND ASTROPHYSICS↗