Results for “flow cell” — Page 42

NASA NTRSDate not supplied

Assessment of Acoustic Behavior for Perforate-Over-Large-Cell Liners

This study explores the effects of increasing the cell size for large-cell acoustic liners. Tests are conducted in the NASA Langley Grazing Flow Impedance Tube to evaluate liners with increasingly larger cell dimensions (up to 2” x 4” cross-section). Conventional impedance eduction confirms that liners with 2” x 3” cells (or larger) must be evaluated using nonlocally reacting assumptions. In addition, due to the sound propagation within cavities, the liners must be modeled using higher fidelity techniques. Thus, grazing flow duct and acoustic liner are modeled simultaneously using finite element methods. The facesheet is modeled using a transfer impedance, while the rest of the domain is modeled using the convected Helmholtz equation. The acoustic pressures predicted are shown to compare favorably with those measured in the Grazing Flow Impedance Tube.

large diameter↗

NASA NTRS2005

Actively Cooled SLMS(TM) Technology for HEL Applications

Mr. Jacoby is the Chief Scientist for Schafer's Lightweight Optical Systems business area with twenty four years experience in laser and optical systems for space and military applications. He and colleague Dr. Goodman conceived and developed Silicon Lightweight Mirrors (SLMS(TM)) technologies for space applications from the extreme UV to FAR IR wavelengths. Schafer has demonstrated two different methods for actively cooling our Silicon Lightweight Mirrors (SLMS(TM)) technology. Direct internal cooling was accomplished by flowing liquid nitrogen through the continuous open cell core of the SLMS(TM) mirror. Indirect external cooling was accomplished by flowing liquid nitrogen through a CTE matched Cesic square-tube manifold that was bonded to the back of the mirror in the center. Testing was done in the small 4-foot thermal/vacuum chamber located at the NASA/MSFC X-Ray Calibration Facility. Seven thermal diodes were located over the front side of the 5 inch diameter mirror and one was placed on the outlet side of the Cesic manifold. Results indicate that the mirror reaches steady state at 82K in less than four minutes for both cooling methods. The maximum temperature difference of the eight diodes was less than 200 mK when the mirror was internally cooled and covered with MLI to insulate it from the large 300 K aluminum plate that was used to mount it.

Jacoby, Marc T.↗

DOE OSTI2025

Disentangling the gap between pure and mixed-gas performance of thin film composite membranes through improved cell design and testing methods

Testing thin film composite (TFC) membrane coupons at low stage-cuts (≤5%) in a sweep-gas permeation system is a common practice to obtain mixed-gas separation properties for benchmarking performance and making scale-up decisions. However, even under these idealized conditions, mixed-gas permeance and selectivity can be more than 30% lower than their pure-gas values, partially due to concentration polarization, an effect that typically intensifies with increased membrane permeance. This study investigates the effect of cell design on mixed-gas testing using PolyActive TM TFC membranes with pure-gas CO 2 permeance of 1700 – 3100 gas permeance unit (GPU), covering the permeance range of most state-of-the-art CO 2 /N 2 separation membranes. Here, we designed and 3D-printed a counter-current permeation cell with enhanced feed and sweep flow efficiency, resulting in a 33 – 41% increase in mixed-gas CO 2 permeance compared to traditional permeation cells. Furthermore, we compared sweep-gas and vacuum permeation methods using traditional permeation cells, revealing that the latter delivers 41% higher mixed-gas CO 2 permeance, because vacuuming effectively minimizes the downstream concentration polarization. These findings highlight the importance of cell design and permeation apparatus selection in lab-scale mixed-gas testing, with strong implications for module design and process optimization at the industrial scale.

mixed gas performance↗

DOE OSTI2020

Cold-hot coupled waves in a flowing magnetized plasma

Abstract Nonlinear coupling of cold and hot waves in a flowing magnetized plasma is analyzed with the Vlasov equation. An analytical solution is obtained for cold waves of a small amplitude (weak flow) and a long wavelength. The distribution function is obtained by integrating the kinetic equation along a perturbed phase-space trajectory for a time-varying plasma flow. The kinetic description presents a generalized dispersion relation that involves resonances depending on cold and hot wave dispersions. Coherent fluid motion leads to radiation peaks in addition to the cyclotron harmonics, where the wavenumber of the cold wave determines the peak frequencies. The peaks appear narrow when the wave propagates perpendicular to the time-averaged flow while they become broad due to the Doppler effect when the wave propagates parallel to the flow. Fully kinetic particle-in-cell simulations corroborate the theoretical predictions. The dispersion relation and resulting wave spectra provide information about plasma parameters and flow properties.

Physics↗

NASA NTRS1993

A review of design concepts for the Advanced Fluids Module (AFM) project

This paper reviews preliminary fluid module design concepts for the Advanced Fluids Module (AFM) project. The objective of this effort is to provide a facility that can handle a wide variety of fluids experiments. Sample science requirements were written and conceptual designs were subsequently generated during the last year. Experiments from the following fluid physics subject areas were used as conceptual design drivers: static and dynamic interfacial phenomena; bubble/droplet thermocapillary migration; surface tension convection and instabilities; thermal/solutal convection; pool boiling; and multiphase flow. After the conceptual designs were completed, the next phase attempted to combine experiments capabilities into a multipurpose, multiuser apparatus configured for the Space Station Freedom. It was found that all the fluid subject areas considered could be accommodated by three basic types of fluids modules. These modules are the Static Fluid Cell Module, the Dynamic Fluid Cell Module, and the Multiphase Flow Module. Descriptions of these preliminary modules designs and their particular sub-systems (e.g., fluid and thermal systems) are discussed. These designs will be refined as the nature of the flight program becomes clearer over the next six to twelve months.

Hill, Myron E.↗

NASA NTRS2019

Weighted Least-Squares Cell-Average Gradient Construction Methods for the VULCAN-CFD Second-Order Accurate Unstructured-Grid Cell-Centered Finite-Volume Solver

The ability to solve the equations governing the hypersonic turbulent flow of a real gas on unstructured grids using a spatially-elliptic, 2nd-order accurate, cell-centered, finite-volume method has been recently implemented in the VULCAN-CFD code. The construction of cell-average gradients using a weighted linear least-squares method and the use of these gradients in the construction of the inviscid fluxes is the focus of this paper. A comparison of least-squares stencil construction methodologies is presented and approaches to augment the number of cells participating in the stencil while preserving accuracy are explored. Due to our interest in hypersonic flow, a robust multidimensional cell-average gradient limiter procedure that is consistent with the stencil used to construct the cell-average gradients is described and investigated. Canonical problems are computed to illustrate the challenges and investigate the accuracy, robustness and convergence behavior of the cell-average gradient methods on unstructured cell-centered finite-volume grids. Finally, thermally perfect, chemically frozen, Mach 8 turbulent flow of air around a blunt wedge is computed to demonstrate the robustness and convergence behavior of the new method for constructing stencils of use in a weighted linear least-squares gradient method for a hypersonic flow.

White, Jeffery A.↗

DOE OSTI2022

Evaluation of bio-inspired flow fields in a mediated Li-S flow battery for grid energy storage

Lithium-sulfur is a redox flow battery with high energy density for applications in safe, reliable, and lasting scaling of energy. However, lithium-based batteries often encounter platting as a problem thanks to poor Li-ions deposition after cycling. Aiming to reduce this impact, a uniform and continuous flow of ions is needed. On this work, novel bio-inspired flow fields in the electrochemical cell were tested to improve ions flowability and lithium platting control, ultimately enhancing battery performance and life. To secure Li-S efficient, low-cost, and secure energy storage capabilities, we chose a configuration with decamethylferrocene and cobaltocene acting as redox mediators, Li metal as anode and sulfur kept in a separate catholyte reservoir. Flow test and battery results insinuated a beneficial influence of bio-inspired designs in flowing electrolyte uniformly with less pressure and pump power in comparison to other conventional designs used in the industry, with an encouraging ability to approach a cheap, safe, and reliable Li-S grid energy storage.

25 ENERGY STORAGE↗

NASA NTRS1985

Accelerated stress testing of amorphous silicon solar cells

A technique for performing accelerated stress tests of large-area thin a-Si solar cells is presented. A computer-controlled short-interval test system employing low-cost ac-powered ELH illumination and a simulated a-Si reference cell (seven individually bandpass-filtered zero-biased crystalline PIN photodiodes) calibrated to the response of an a-Si control cell is described and illustrated with flow diagrams, drawings, and graphs. Preliminary results indicate that while most tests of a program developed for c-Si cells are applicable to a-Si cells, spurious degradation may appear in a-Si cells tested at temperatures above 130 C.

Stoddard, W. G.↗

NASA NTRS1992

Gallery of fluid motion

Winning photographs from the ninth Annual Fluid Mechanics Photo Contest held by the Division of Fluid Dynamics of the American Physical Society in November 1991 are displayed. Pictures are presented of vortex flows, a periodic axisymmetric vortex breakdown in a cylinder with a rotating end wall, impacting water drops, a vortex structure behind a disk started from rest, dynamic stall, blue waves, isotherms in turbulent Rayleigh-Benard convection, a square jet, 'vapor lines' emanating from water droplets, viscosity effects on the directional solidification of NH4Cl solution in a Hele-Shaw cell, and stationary cross-flow vortices.

Reed, Helen L.↗

DOE OSTI2020

The Effects of Absorber Thickness on Reverse-Bias Damage in Cu(In,Ga)Se2 Solar Cells

We studied a variety of absorber thicknesses in Cu(In,Ga)Se 2 devices to examine the ability of the cell to withstand reverse current flow without damage. Absorbers were varied from 2.5 um to 0.4 um, with the expectation that larger reverse current would be allowed to flow at lower voltages as absorbers were made thinner. Our initial experiments showed promise. However, as the efficiency of thin devices was improved, the reverse current allowed at a given voltage decreased. Here we present our negative results on the ability of thin devices to allow higher reverse currents at lower voltages. We also give details on our procedures for reverse-bias testing small-area solar cells. We hope this information will be useful for those who want to study reverse-bias at the cell level.

14 SOLAR ENERGY↗

NASA NTRS1993

Electrophoretic separation of cells and particles from rat pituitary and rat spleen

There are 3 parts to the IML-2 TX-101 experiment. Part 1 is a pituitary cell culture experiment. Part 2 is a pituitary cell separation experiment using the Japanese free flow electrophoresis unit (FFEU). Part 3 is a pituitary secretory granule separation experiment using the FFEU. The objectives of this three part experiment are: (1) to determine the kinetics of production of biologically active growth hormone (GH) and prolactin (PRL) in rat pituitary GH and PRL cells in microgravity (micro-g); (2) to investigate three mechanisms by which a micro-g-induced lesion in hormone production may occur; and (3) to determine the quality of separations of pituitary cells and organelles by continuous flow electrophoresis (CFE) in micro-g under conditions where buoyancy-induced convection is eliminated.

Hymer, Wesley C.↗

NASA NTRS1999

Microgravity Transport Phenomena Experiment (MTPE) Overview

The Microgravity Transport Phenomena Experiment (MTPE) is a fluids experiment supported by the Fundamentals in Biotechnology program in association with the Human Exploration and Development of Space (BEDS) initiative. The MTP Experiment will investigate fluid transport phenomena both in ground based experiments and in the microgravity environment. Many fluid transport processes are affected by gravity. Osmotic flux kinetics in planar membrane systems have been shown to be influenced by gravimetric orientation, either through convective mixing caused by unstably stratified fluid layers, or through a stable fluid boundary layer structure that forms in association with the membrane. Coupled transport phenomena also show gravity related effects. Coefficients associated with coupled transport processes are defined in terms of a steady state condition. Buoyancy (gravity) driven convection interferes with the attainment of steady state, and the measurement of coupled processes. The MTP Experiment measures the kinetics of molecular migration that occurs in fluids, in response to the application of various driving potentials. Three separate driving potentials may be applied to the MTP Experiment fluids, either singly or in combination. The driving potentials include chemical potential, thermal potential, and electrical potential. Two separate fluid arrangements are used to study membrane mediated and bulk fluid transport phenomena. Transport processes of interest in membrane mediated systems include diffusion, osmosis, and streaming potential. Bulk fluid processes of interest include coupled phenomena such as the Soret Effect, Dufour Effect, Donnan Effect, and thermal diffusion potential. MTP Experiments are performed in the Microgravity Transport Apparatus (MTA), an instrument that has been developed specifically for precision measurement of transport processes. Experiment fluids are contained within the MTA fluid cells, designed to create a one dimensional flow geometry of constant cross sectional area, and to facilitate fluid filling and draining operations in microgravity. The fluid cells may be used singly for bulk solutions, or in a Stokes diaphragm configuration to investigate membrane mediated phenomena. Thermal and electrical driving potentials are applied to the experiment fluids through boundary plates located at the ends of the fluid cells. In the ground based instrument, two constant temperature baths circulate through reservoirs adjacent to the boundary plates, and establish the thermal environment within the fluid cells. The boundary plates also serve as electrodes for measurement and application of electrical potentials. The Fluid Manipulation System associated with the MTA is a computer controlled system that enables storage and transfer of experiment fluids during on orbit operations. The system is used to automatically initiate experiments and manipulate fluids by orchestrating pump and valve operations through scripted sequences. Unique technologies are incorporated in the MTA for measurement of fluid properties. Volumetric Flow Sensors have been developed for precision measurement of total fluid volume contained within the fluid cells over time. This data is most useful for measuring the kinetics of osmosis, where fluid is transported from one fluid cell to another through a semipermeable membrane. The MicroSensor Array has been designed to perform in situ measurement of several important fluid parameters, providing simultaneous measurement of solution composition at multiple locations within the experiment fluids. Micromachined sensors and interface electronics have been developed to measure temperature, electrical conductivity, pH, cation activity, and anion activity. The Profile Refractometer uses a laser optical system to directly image the fluid Index of Refraction profile that exists along the MTA fluid cell axis. A video system acquires images of the RI profile over time, and records the transport kinetics that occur upon application of chemical, thermal, or electrical driving potentials. Image processing algorithms have been developed to analyze the refractometer images on a pixel by pixel basis, calibrating and scaling the measured Index of Refraction profile to correlated solution properties of interest such as density, concentration, and temperature. Additional software has been developed to compile the processed images into a three dimensional matrix that contains fluid composition data as a function of experiment time and position in the fluid cell. These data are combined with data from the other sensor systems, and analyzed in the context of transport coefficients associated with the various transport phenomena. Analysis protocols have been developed to measure the transient kinetics, and steady state distribution of fluid components that occur in response to the applied driving potentials. The results are expressed in terms of effective transport coefficients. Experiments have been performed using a variety of solutes, and results generated are that are in agreement with published transport coefficient values.

Mason, Larry W.↗

NASA NTRS1979

The Production of Solar Cell Grade Silicon from Bromosilanes

A continuous Flow Reactor (CFR) process based on the hydrogen reduction of the bromosilanes SiBr4 and SiHBr3 was proposed. Initial experiments carried and directed at obtaining overall yield data for bromosilane reduction in the CFR, indicated the need for increased reactor residence time and deposition substrate particle packing density to fully characterize the kinetics (rate) and thermodynamics (yield) of observed silicon production. Fluidized bed experiments were therefore initiated to overcome these experimental difficulties, which showed both thermal decomposition and hydrogen reduction of SiHBr3 in a fluid bed reactor to present attractive closed-loop processes for producing solar cell grade polycrystalline silicon. No process selection could be made however due to the fact that preliminary optimization of 2 of 3 process stages in each case during the course of the experimental program showed comparable attainment of cost element objectives.

Schumacher, J. C.↗

DOE OSTI2021

Sensitivity Analysis of MFiX-PIC Parameters Using Nodeworks, PSUADE, and DAKOTA

The study presented in this report was aimed to demonstrate UQ analysis performed not only with Nodeworks, but also two other well-established UQ software tools from the U.S. DOE’s National Laboratories (PSUADE from Lawrence Livermore National Laboratory and DAKOTA from Sandia National Laboratory). It is important to emphasize that the motivation of this study was not to determine the best UQ software, but to verify if the global sensitivity analyses from the end-to-end workflow in Nodeworks are consistent with the results of other two UQ software. The components of Nodeworks from Python’s ecosystem have been tested as standalone libraries. However, an assessment study for the complete workflow targeting a specific UQ analysis has not been performed for Nodeworks. Hence, this study is expected to serve as an equivalent of solution verification for Nodeworks using other established UQ tools as reference solution. For this purpose, three distinct flow configurations (i.e., settling bed, bubbling fluidized, and circulating fluidized bed) have been used as representative multiphase flow problems of interest. The results of the systematic simulation campaigns performed in an earlier study using the particle-in-cell (PIC) approach in the Multiphase Flow with Interphase eXchanges (MFIX) suite of solvers (i.e., MFiX-PIC) was utilized. The same set of tabulated results was provided as input to the different UQ software for global sensitivity analysis. Results for the three cases indicate that based on the Sobol’ Sensitivity Indices method the order of importance ranking determined by Nodeworks for the Sobol’ Total Sensitivity Indices is consistent with PSUADE and DAKOTA in each case for the five model parameters considered. The input files for Nodeworks for the three cases are also shared through NETL’s Gitlab repository for the reader interested in reproducibility and further analysis (See Section 1.2).

97 MATHEMATICS AND COMPUTING↗

NASA NTRS1992

Prolactin variants in ram adenohypophyses vary with season

Secretion of PRL in sheep is affected by photoperiod being highest during the spring and summer, lowest in fall and winter. The objectives of this study were to determine if 1) the production of variant forms of PRL, and 2) immuno- and bioactivities of PRL (iPRL and bPRL) differ during times of the year selected to represent periods of low, transitional and high PRL secretion. Twelve mature rams were maintained on pasture and killed in October, December, and April (n = 4/month). Individual pituitary glands were dispersed, cells obtained, and fixed for immunocytochemical flow cytometry, extracted with 0.01 N NaHCO3 or cultured in serum-free, defined media. The Mr of PRL extracted from cells immediately following dispersion ranged from 14-140K, with significantly more bands greater than 40K being detected from rams sacrificed in December than from those killed in October and April (P less than 0.01). No bands of PRL greater than 25K were observed when samples were reduced with beta-mercaptoethanol prior to electrophoresis, indicating that the high Mr forms were disulfide-linked aggregates. Culture media from October and April contained variants of PRL that ranged from 22-40K but those greater than 25K were generally not observed from cells harvested during December. Extracts of cells after 24 h in culture contained fewer high Mr species during December than had been present in initial extracts from that month. In contrast, during April more high Mr intracellular forms were present after culture than had been detected prior to culture during that month. The percentage of lactotrophs averaged 50.0 +/- 2.5, 47.4 +/- 5.7, and 59.4 +/- 5.5 for October, December, and April, respectively. Initial lactotroph content (pg/lactotroph) of iPRL was higher (P = 0.06) in April (46.0 +/- 17.0) when compared to October and December (8.0 +/- 2.0 and 20.0 +/- 10.0, respectively). In contrast, the bPRL content of initial extracts was higher (P = 0.05) in December (267.0 +/- 68.0) than in October (101.0 +/- 35.0), but not than in April (190.0 +/- 70.0). Although iPRL and bPRL concentrations in culture media were similar for the 3 months, the intracellular iPRL (P less than 0.001) and bPRL (P less than 0.0001) content after culture was greatest during April. In summary, in addition to the well-documented seasonal changes in blood concentrations of PRL, different molecular forms of PRL were found within the pituitary at different times of the year and seasonal variations in iPRL and bPRL did not occur in parallel.

Non-NASA Center↗

NASA NTRSDate not supplied

Role of PIEZO1 in T Cell Activation Under Simulated Microgravity

True and simulated microgravity conditions have been well documented to cause the inhibition of T cell activation by mitogens. Although several studies aimed at exploring the mechanisms for such a phenomenon have been published, how this is occurring remains unresolved. PIEZO1 is a known mechanosensing gene and has been shown to be critically involved in human T cell activation. In our analysis of transcriptomics changes in peripheral mononuclear cells (PBMC) collected from the ISS crewmembers in space, the expression of PIEZO1 was downregulated. To investigate the role of the PIEZO1 gene in T cell activation in microgravity, we used rotating wall vessels (RWV), which simulate microgravity on the ground and are known to inhibit T cell activation. In this pilot study, PBMC cultured in RWV and in the static 1g condition were stimulated with Human T-Activator CD3/CD28 beads. The cells were also treated with and without Yoda1, a chemical agonist that activates PIEZO1 independent of mechanical cues or any other cellular component. After culturing for 24 hours, the cells were stained for activation markers and the PIEZO1 antibody, then were analyzed by flow cytometry. Our results indicate reduced T cell activation with mitogen under simulated microgravity, but no changes of the PIEZO1 signals were detected. In addition, the reduced activation was not restored in cells cultured with Yoda1. Taken together, our results suggest that PIEZO1 may play a minimal role in the inhibition of T cell activation in space.

Honglu Wu↗

NASA NTRSDate not supplied

Role of PIEZO1 in T Cell Activation Under Simulated Microgravity

True and simulated microgravity conditions have been well documented to cause the inhibition of T cell activation by mitogens. Although several studies aimed at exploring the mechanisms for such a phenomenon have been published, how this is occurring remains unresolved. PIEZO1 is a known mechanosensing gene and has been shown to be critically involved in human T cell activation. In our analysis of transcriptomics changes in peripheral mononuclear cells (PBMC) collected from the ISS crewmembers in space, the expression of PIEZO1 was downregulated. To investigate the role of the PIEZO1 gene in T cell activation in microgravity, we used rotating wall vessels (RWV), which simulate microgravity on the ground and are known to inhibit T cell activation. In this pilot study, PBMC cultured in RWV and in the static 1g condition were stimulated with Human T-Activator CD3/CD28 beads. The cells were also treated with and without Yoda1, a chemical agonist that activates PIEZO1 independent of mechanical cues or any other cellular component. After culturing for 24 hours, the cells were stained for activation markers and the PIEZO1 antibody, then were analyzed by flow cytometry. Our results indicate reduced T cell activation with mitogen under simulated microgravity, but no changes of the PIEZO1 signals were detected. In addition, the reduced activation was not restored in cells cultured with Yoda1. Taken together, our results suggest that PIEZO1 may play a minimal role in the inhibition of T cell activation in space.

Honglu Wu↗

NASA NTRSDate not supplied

Role of PIEZO1 in T Cell Activation Under Simulated Microgravity

True and simulated microgravity conditions have been well documented to cause the inhibition of T cell activation by mitogens. Although several studies aimed at exploring the mechanisms for such a phenomenon have been published, how this is occurring remains unresolved. PIEZO1 is a known mechanosensing gene and has been shown to be critically involved in human T cell activation. In our analysis of transcriptomics changes in peripheral mononuclear cells (PBMC) collected from the ISS crewmembers in space, the expression of PIEZO1 was downregulated. To investigate the role of the PIEZO1 gene in T cell activation in microgravity, we used rotating wall vessels (RWV), which simulate microgravity on the ground and are known to inhibit T cell activation. In this pilot study, PBMC cultured in RWV and in the static 1g condition were stimulated with Human T-Activator CD3/CD28 beads. The cells were also treated with and without Yoda1, a chemical agonist that activates PIEZO1 independent of mechanical cues or any other cellular component. After culturing for 24 hours, the cells were stained for activation markers and the PIEZO1 antibody, then were analyzed by flow cytometry. Our results indicate reduced T cell activation with mitogen under simulated microgravity, but no changes of the PIEZO1 signals were detected. In addition, the reduced activation was not restored in cells cultured with Yoda1. Taken together, our results suggest that PIEZO1 may play a minimal role in the inhibition of T cell activation in space.

Honglu Wu↗