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At least 757 records · Page 42

Simulation of shading and algal growth in experimental raceways

Many algae research groups use elevated experimental raceways to characterize algal biomass productivity. Side walls and a relatively large paddlewheel shade the culture and lower productivity, particularly in winter and with low culture depth. This paper presents a methodology to calculate shading for a given raceway shape: (1) develop a mesh of the inner surface geometry of the raceway; (2) offset the mesh horizontally in the x and y directions on the water surface as a function of wall height, solar zenith angle, and solar azimuth angle; (3) calculate the shaded area for each projected mesh with the shoelace algorithm; (4) use regression to develop a response surface for shaded area as a function of horizontal x and y offset. For each time step, the shaded area is calculated with the regression equation based on the x and y offset at that time step. The shading model was added to the Huesemann Algae Biomass Growth (HABG) model, which predicts algae growth rate as a function of water temperature and light intensity in algae culture layers. The shading model was evaluated with biomass concentrations from three Regional Algal Feedstock Testbed (RAFT) experiments with three different species of algae. The shading algorithm reduced the HABG estimate of productivity and improved the agreement with observed productivities. In the model, productivity was sensitive to the assumed light distribution below the water surface. Averaging light in each layer resulted in higher calculated growth rate than calculating growth separately in shaded and unshaded areas. Shaded area was sensitive to raceway orientation, water depth, and time of year. A north-south raceway orientation had less average shading in winter but more average shading in summer than an east-west orientation.

59 BASIC BIOLOGICAL SCIENCES↗

Timing is everything: diel metabolic and physiological changes in the diatom Cyclotella cryptica grown in simulated outdoor conditions

Microalgal cultures grown on a light-dark cycle experience diel patterns in metabolic and physiological processes, including cell cycle synchronization, but the implications for productivity in terms of biomass and commerciallyappealing molecules are not commonly appreciated. Despite a long history of diel response studies, only recently have photobioreactor technology advances enabled the use of sinusoidal light and temperature to more accurately mimic outdoor conditions. The present study investigates cell cycle progression and dynamic changes in biomass, triacylglycerol (TAG), and photosynthetic pigments on a 24-hour scale in the diatom Cyclotella cryptica grown using a sinusoidal light and temperature regime. Cell division synchronized to occur predominantly in the middle of the light period and biomass started to increase several hours earlier, as the cells prepared to divide. TAG levels increased during the day and decreased at night, with a mid-day dip corresponding to the time when lipid needs for cell divisionassociated membrane biosynthesis would be high. For the first time, photosynthetic pigment dynamics data, obtained with higher temporal resolution than previously reported for microalgae, was overlaid with cell cycle progression, indicating that while some photosynthetic pigments respond primarily to light, others are influenced by the cell cycle. Another novel observation was that the abundance of most photosynthetic pigments also fluctuated substantially on a rapid time scale during dawn, dusk, and the beginning of the dark period. Additionally, the results indicate that in a synchronized culture, potential product yields change substantially throughout the day. This may inform harvest timing to significantly increase yield.

microalgae, biofuels, Cell Lipids↗

Beyond the standard two-film theory: Computational fluid dynamics simulations for carbon dioxide capture in a wetted wall column

The standard two-film theory (STFT) is a diffusion-based mechanism that can be used to describe gas mass transfer across liquid film. Fundamental assumptions of the STFT impose serious limitations on its ability to predict mass transfer coefficients. To better understand gas absorption across liquid film in practical situations, a multiphase computational fluid dynamics (CFD) model fully equipped with mass transport and chemistry capabilities has been developed for solvent-based carbon dioxide (CO2) capture to predict the CO2 mass transfer coefficient in a wetted wall column. The hydrodynamics is modeled using a volume of fluid method, and the diffusive and reactive mass transfer between the two phases is modeled by adopting a one-fluid formulation. We demonstrate that the proposed CFD model can naturally account for the influence of many important factors on the overall mass transfer that cannot be quantitatively explained by the STFT, such as the local variation in fluid velocities and properties, flow instabilities, and complex geometries. The CFD model also can predict the local mass transfer coefficient variation along the column height, which the STFT typically does not consider.

Wang, Chao↗