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At least 757 records · Page 42

Quality Assurance and Quality Control (QA/QC) of Meteorological Time Series Data for Billy Barr, East River, Colorado USA

A comprehensive Quality Assurance (QA) and Quality Control (QC) statistical framework consists of three major phases: Phase 1—Preliminary raw data sets exploration, including time formatting and combining datasets of different lengths and different time intervals; Phase 2—QA of the datasets, including detecting and flagging of duplicates, outliers, and extreme values; and Phase 3—the development of time series of a desired frequency, imputation of missing values, visualization and a final statistical summary. The time series data collected at the Billy Barr meteorological station (East River Watershed, Colorado) were analyzed. The developed statistical framework is suitable for both real-time and post-data-collection QA/QC analysis of meteorological datasets.The files that are in this data package include one excel file, converted to CSV format (Billy_Barr_raw_qaqc.csv) that contains the raw meteorological data, i.e., input data used for the QA/QC analysis. The second CSV file (Billy_Barr_1hr.csv) is the QA/QC and flagged meteorological data, i.e., output data from the QA/QC analysis. The last file (QAQC_Billy_Barr_2021-03-22.R) is a script written in R that implements the QA/QC and flagging process. The purpose of the CSV data files included in this package is to provide input and output files implemented in the R script.

54 ENVIRONMENTAL SCIENCES↗

Kinetic and temperature sensitivity properties of soil exoenzymes through the soil profile down to one-meter depth at a temperate coniferous forest (Blodgett, CA)

This dataset contains data on kinetic and temperature sensitivity parameters of the exoenzymes β-glucosidase (BG), leucine/leucyl aminopeptidase (LAP) and acid phosphatase (AP) across a soil profile down to 90 cm depth at Blodgett forest, CA, USA. These data were generated to determine if kinetic and thermal properties of microbial exoenzymes involved in organic matter decomposition varied with soil depth, following variation in soil properties and microbial communities, as part of the study: Alves et al. (2021). Kinetic Properties of Microbial Exoenzymes Vary with Soil Depth but Have Similar Temperature Sensitivities Through the Soil Profile. Frontiers in Microbiology 12:3618. https://doi.org/10.3389/fmicb.2021.735282. This research was performed within the framework of the TES Belowground Biogeochemistry SFA project, in particular association with a long-term field warming experiment of the whole soil profile at Blodgett forest. Samples for this work were collected from locations representative of the field experimental plots. Potential enzyme activity rates were measured using laboratory fluorometric assays with soils collected at 0-10, 10-20, 30-40, 50-60, 60-70 and 80-90 cm deep in biological triplicates (i.e., three soil cores collected at different representative locations). Assays with each soil were conducted over a gradient of eight substrate concentrations per enzyme, and incubated at 4, 10, 16, 25, 35 or 50°C. Enzyme Michaelis-Menten kinetics were modeled over the eight substrate concentrations at each temperature, and the temperature sensitivity of the kinetic parameters was modeled over the six temperatures using linear Arrhenius/Q10 and non-linear Macromolecular Rate Theory (MMRT) models. The dataset includes the fully processed enzyme activity rate data used to model Michaelis-Menten kinetics, calculated kinetic and temperature sensitivity parameters, and basic soil and microbial biomass chemistry for each sample. All data is provided for each individual biological replicate, and kinetic and temperature sensitivity parameters are also provided as means of the biological replicates. The dataset also includes all raw measurement data and code used to parse, combine and perform the analyses described by Alves et al. (2021). For file descriptions, see the file-level metadata files: “enzymes_dataProcessed_flmd.xlsx” (processed data); “enzymes_dataRaw_flmd.xlsx” (compressed raw data and metadata); and “enzymes_code_flmd.xlsx” (compressed code). The experimental design, list of parameters measured, soil and microbial biomass chemistry data, and means of biological replicates for kinetic and temperature sensitivity parameters are also provided as human-readable tables in file “enzymeTraits_design_results_tables.xlsx”.

54 ENVIRONMENTAL SCIENCES↗

FTICR-MS and Biochemical Transformation Data from Global Inland River Water and Sediment Associated with: "Organic Matter Transformations are Disconnected Between Surface Water and the Hyporheic Zone"

This data package is associated with the publication "Organic Matter Transformations are Disconnected Between Surface Water and the Hyporheic Zone" submitted to Biogeosciences (Stegen et al., 2022). The study aims to understand how the diversity of OM transformations varies across surface and subsurface components of river corridors using inland surface water and sediments collected along river corridors across the contiguous United States. Sediment extracts and water samples were analyzed using ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). This dataset is comprised of one folder (WHONDR_S19S) which contains (1) a subfolder with R scripts used to process the data and to calculate biochemical transformations, (2) processed FTICR-MS data as csv files, sample collection metadata and climate data as csv files, (3) biochemical transformations profile, classifications and database as csv files, and (4) a readme file with more information regarding WHONDRS raw FTICR-MS data and processing scripts. Outside of the main folders there is a csv containing file-level metadata and a csv data dictionary defining column headers for all csv files contained in the data package. The samples were part of a WHONDRS (https://whondrs.pnnl.gov) study. The raw, unprocessed FTICR-MS data with additional data can be found at doi:10.15485/1729719 for sediments and doi:10.15485/1603775 for water. This data package contains the processed data used in the associated manuscript.

54 ENVIRONMENTAL SCIENCES↗

16S and ITS Amplicon Sequencing Fastq files and metadata from PARCHED Panama Tropical Forest soils, 2019-2020,

Model projections predict tropical forests will experience longer periods of drought and more intense precipitation cycles under a changing climate. Such transitions have implications for structure-function relationships within microbial communities. We examine how chronic drying might reshape prokaryotic and fungal communities across four lowland forests in Panama with a wide variation in mean annual precipitation and soil fertility. Four sites were established across a 1000 mm span in mean annual precipitation (2335 to 3300 mm). We expected microbial communities at sites with lower MAP to be less sensitive to chronic drying than sites with higher MAP; while fungal communities to be more resistant to disturbance than prokaryotes. At each location, partial throughfall exclusion structures were established over 10 x 10 m plots to reduce direct precipitation input. Raw demultiplexed sequences (bacteria, archaea, fungal) from soil samples taken from PARCHED Panama Tropical Forest throughfall exclusion experiments. Files that contain 16S are sequences from prokaryotes, ITS indicates sequences from fungi. Compressed fastq files are contained in Field_PARCHED_ITS_fastq_2020.zip, Field_PARCHED_ITS_fastq_2019.zip, Field_PARCHED_16S_fastq_2020.zip, Field_PARCHED_16S_fastq_2019.zip.There are four sites across the isthmus of Panama, each with 4 control and 4 exclusion plots. Throughfall exclusion shelters were built to intercept 50% of throughfall precipitation that hits the soil. Samples were taken on May 2019 and Jan 2020 from 0-10 cm and 10-20 cm depth approximately 9 and 18 months after shelter installation. Metadata and sample IDs for fastq files for 2019 sampling are within Field_PARCHED_Metadata_2019_ITS.csv and Field_PARCHED_Metadata_2019_16S.csv. The metadata for both 16S and ITS fastq files for January 2020 is included in Field_PARCHED_Metadata_2020.csv. No data processing or QA/QC was done on the raw data. Data processing example provided in R notebook file.

54 ENVIRONMENTAL SCIENCES↗

Dataset for 'Ombadi et al. (2023). A warming-induced reduction in snow fraction amplifies rainfall extremes, Nature'

This package contains the main codes, sample input data and main result files to reproduce the analysis and results presented in the article: “Ombadi et al. (2023), A warming-induced reduction in snow fraction amplifies rainfall extremes, Nature”. The folder consists of the following: (1) “Raw data”: a folder that contains sample input data which is used in some of the codes for demonstration purposes. It also contains data that was not pre-processed such as Elevation data; (2) “Results”: this folder contains files of the main results presented in the paper including: “Annual-Max-Series”, “Change-rainfall-extremes”, “Change-snow-fraction”, “Warming levels_By scenario_model_year” and “Masks”. Description of these folders is detailed in the "Readme.rtf" file; (3) Python jupyter notebooks (Extract_Annual Max Series (AMS).ipynb, Elevation-dependent amplification of rainfall extremes.ipynb, Sensitivity_to_global_warming.ipynb) demonstrate the main steps of analysis. Further description of those notebooks is provided in the "Readme.rtf" file; (4) R code for extreme value analysis (Extreme_Value_Analysis.R). The sample and pre-processed dataset in "Raw data" is obtained from publicly available repositories of CMIP6 and ERA5 datasets; see Methods for more detail. This research was supported by Office of Science, Office of Biological and Environmental Research of the US Department of Energy under contract no. DE-AC02-05CH11231 for the CASCADE Scientific Focus (funded by the Regional and Global Model Analysis Program area within the Earth and Environmental Systems Modeling Program) and the iNAIADS Early Career Research Project (funded by the Environmental Systems Science program).

54 ENVIRONMENTAL SCIENCES↗

UAS remote sensing (Autel EVO II platform): Red-green-blue (RGB) imagery and derived products (Level 0-2 data), Seward Peninsula, Alaska, 2022

Airborne remote sensing data collected using a optical red-green-blue (RGB) sensor installed on an Autel EVO II unoccupied aerial system (UAS). This package includes data from 15 flights flown over the NGEE-Arctic Council Mile Marker (MM) 71, Kougarok MM64, and Teller MM27 sites on the Seward Peninsula, Alaska USA, in July 2022. This package provides the Level 0 (raw, unprocessed) data collected by the platform and sensors, and Level 1-2 processed products including digital surface models (DSM), photo orthomosaics, canopy height models (CHM) and digital terrain models (DTM). Ancillary aircraft data, flight mission parameters, and general flight conditions provided by the onboard flight and data collection computers are also included. Data and metadata are provided as image (*.jpg, *.tif), point cloud (*.laz), tabular (*.csv) and *.pdf formats. Raw images are compressed as tar.gz. The metadata documents contain flight campaign, platform, sensor, flight and file metadata, along with a description of the data and file naming scheme.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a 15-year research effort (2012-2027) to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

UAS remote sensing (Autel EVO II platform): Red-green-blue (RGB) imagery and derived products (Level 0-2 data), Seward Peninsula, Alaska, 2023

Airborne remote sensing data collected using a optical red-green-blue (RGB) sensor installed on an Autel EVO II unoccupied aerial system (UAS). This package includes data from 4 flights flown over the NGEE-Arctic Council Mile Marker (MM) 71 and Teller MM27 sites on the Seward Peninsula, Alaska USA, in July 2023. This package provides the Level 0 (raw, unprocessed) data collected by the platform and sensors, and Level 1-2 processed products including digital surface models (DSM), photo orthomosaics, canopy height models (CHM) and digital terrain models (DTM). Ancillary aircraft data, flight mission parameters, and general flight conditions provided by the onboard flight and data collection computers are also included. Data and metadata are provided as image (*.jpg, *.tif), point cloud (*.laz), tabular (*.csv) and *.pdf formats. Raw images are compressed as tar.gz. The metadata documents contain flight campaign, platform, sensor, flight and file metadata, along with a description of the data and file naming scheme.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a 15-year research effort (2012-2027) to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Seedling nitrogen uptake and rhizodeposition between mycorrhizal types

Tree mycorrhizal associations are associated with patterns in N cycling and soil organic matter (SOM) storage, however, we still lack a mechanistic understanding of what tree and fungal traits drive these patterns and how they will respond to global changes in soil N availability. To address this knowledge gap, we investigated how arbuscular mycorrhizal (AM)- and ectomycorrhizal (EcM)-associated seedlings alter rhizodeposition in response to increased inorganic N acquisition. Specifically, we conducted this greenhouse experiment in a sealed labeling chamber with an enriched 13Carbon atmosphere and 15Nitrogen enriched fertilizer over the course of five months from April 2021 - August 2021. To include the variability across tree species, we grew eight species of seedlings belonging to eight families that were either arbuscular (Acer rubrum, Nyssa sylvatica, Thuja occidentalis, and Prunus seritina) or ectomycorrhizal-associated (Quercus rubra, Tilia americana, Pinus strobus, and Betula lenta). We measured rhizodeposition (mg 13C), plant N uptake from fertilizer (mg N), net soil carbon, and the abundance of mycorrhizal fungi (ITS sequencing and qPCR). We also characterized fungal (ITS2) and bacterial (16S) soil communities.The data from this project are ".csv" files that can up downloaded into a folder, and then run in the associated R markdown scripts after changing the source folder location at the top of the script. These data include raw outputs and processed files (using the R markdown files) for seedling growth and biomass, 15N content, soil 13C content, raw reads and processed file versions for fungal and bacterial ASVS, and a final summary file used for modeling. R software is needed to run these data, and the packages needed are listed at the top of the R markdown file.

54 ENVIRONMENTAL SCIENCES↗

Dendrometer data at The Morton Arboretum Forestry Plots 2019-2023

We are collecting long-term dendrometer data at The Morton Arboretum to determine seasonal growth patterns in trees. This data on stem growth patterns will eventually be integrated with other ongoing data streams to paint a broader picture of plant phenology. This data package contains the outputs from three types of dendrometer devices: ICT band dendrometer data is in the "ICT_data2020_2023.csv" file, TreeHugger band dendrometer data is in the "TreeHugger_data2019_2020.csv" file, and TOMST point dendrometer data is in the "TOMST_data2021_2023.csv" file. The TreeHugger and TOMST files contain corrected and raw uncorrected values, whereas the ICT data file contains only raw data. Initial tree size data at device installation is located in the "Initial_Tree_Size.csv" file. Additional information on units are contained within each data file's respective data dictionary, and the location metadata file contains the geographic locations of the 23 forestry plots at The Morton Arboretum as well as the measured tree species at each location.

54 ENVIRONMENTAL SCIENCES↗

Data and scripts associated with the manuscript "Organic Molecules are Deterministically Assembled in River Sediments"

This data package is associated with the publication "Organic Molecules are Deterministically Assembled in River Sediments" submitted to Scientific Reports (Stegen et al., 2024). The study applies community ecology methods to dissolved organic matter (DOM) chemistry from variably inundated riverbed sediments to uncover principles governing DOM composition at a reach-scale. This data package documents the workflow used to process and generate the main findings in the manuscript. The R scripts reference the raw, unprocessed Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) data from another data package, available on ESS-DIVE at https://data.ess-dive.lbl.gov/view/doi:10.15485/1834208. The scripts then process the raw FTICR-MS data and generate the findings and figures presented in the associated manuscript. In brief, this study demonstrates that DOM assemblages in variably inundated sediments are primarily governed by deterministic variable selection, including sediment moisture effecting the degree of deterministic assembly. See the manuscript for more details pertaining to interpretation and implications of the findings. This data package is associated with the GitHub repository found at https://github.com/WHONDRS-Hub/ECA_2020_Sed.This data package is comprised of 6 scripts and 7 folders. The file-level metadata file (file ending in "flmd.csv") lists all files contained in this data package and descriptions for each. The data dictionary (file ending in "dd.csv) describes all tabular data columns and their respective definitions and units. The FTICR_Processing_Scripts produce the outputs found in the "Processed_Data" folder. The remaining scripts (located in the parent directory) produce the outputs found in the following four folders: (1) "MCD_Dendrograms", "MCD_Randomizations", "MCD_bNTI_Outcomes", and "OM_Null_Modeling". The fifth script additionally takes the three comma-separated values (CSV) files found in the parent directory as input ("VGC_texture.csv", "merged_weights.csv", and "ECA2_FTICR_BetaDisp.csv"). The outputs of each of the five scripts serve as the input to the following script, with the final outputs stored in the folder "OM_Null_Modeling".

54 ENVIRONMENTAL SCIENCES↗

UAS remote sensing (AltaX platform): Red-green-blue (RGB) imagery, thermal infrared (TIR) imagery, and canopy reflectance (Level 0 data), Seward Peninsula, Alaska, 2023

Airborne remote sensing data collected using Brookhaven National Laboratory’s (BNL) heavy-lift unoccupied aerial system (UAS) AltaX platform on the Seward Peninsula, Alaska, USA. This package includes raw (L0) data from 11 flights flown over the NGEE-Arctic Kougarok mile marker 64 (MM64) and Teller MM 27 sites in July, 2023. The AltaX is a multi-sensor UAS platform that simultaneously measures very high spatial resolution optical red/green/blue (RGB) and thermal infrared (TIR) surface “skin” temperature imagery, as well as near-surface surface reflectance at 1 nm intervals in the visible to near-infrared spectral range from ~350 – 1000 nm. This package provides the Level 0 (raw, unprocessed) data collected by the platform and sensors. Ancillary aircraft data, flight mission parameters, and general flight conditions provided by the onboard flight and data collection computers are also included. Data and metadata are provided as text (*.txt, *.json), binary (*.dat, *.ulg), tabular (*.csv), geospatial (*.kml. *kmz), image (*.jpg, *.png, *.tif) and pdf formats. The metadata documents contain flight campaign, platform, sensor, flight and file metadata, along with a description of the L0 data and the file naming scheme.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a 15-year research effort (2012-2027) to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

UAS remote sensing (AltaX platform): Red-green-blue (RGB) imagery, thermal infrared (TIR) imagery, and canopy reflectance (Level 0 data), Seward Peninsula, Alaska, 2022

Airborne remote sensing data collected using Brookhaven National Laboratory’s (BNL) heavy-lift unoccupied aerial system (UAS) AltaX platform on the Seward Peninsula, Alaska, USA. This package includes raw (L0) data from 10 flights flown over the NGEE-Arctic Kougarok mile marker 64 (MM64) and Teller MM 27 sites in July, 2022. The AltaX is a multi-sensor UAS platform that simultaneously measures very high spatial resolution optical red/green/blue (RGB) and thermal infrared (TIR) surface “skin” temperature imagery, as well as near-surface surface reflectance at 1 nm intervals in the visible to near-infrared spectral range from ~350 – 1000 nm. This package provides the Level 0 (raw, unprocessed) data collected by the platform and sensors. Ancillary aircraft data, flight mission parameters, and general flight conditions provided by the onboard flight and data collection computers are also included. Data and metadata are provided as text (*.txt, *.json), binary (*.dat, *.ulg), tabular (*.csv), geospatial (*.kml. *kmz), image (*.jpg, *.png, *.tif) and pdf formats. The metadata documents contain flight campaign, platform, sensor, flight and file metadata, along with a description of the L0 data and the file naming scheme.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a 15-year research effort (2012-2027) to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Laboratory time series moisture manipulative experiment from sediment across the contiguous US: time series aerobic respiration and geochemistry (v2)

This dataset supports a broader study examining the effects of wetting and drying on hyporheic zone respiration across the contiguous United States (CONUS). The dataset provides data generated from a laboratory moisture manipulation experiment. The contents include time series aerobic respiration and moisture; dissolved oxygen; sediment geochemistry data; and field metadata (including qualitative information on instream and river corridor characteristics). Samples were collected as part of the WHONDRS CONUS-Scale Model-Sample Study (CM). This study was designed following ICON (integrated, coordinated, open, and networked) principles to facilitate a model-experiment (ModEx) iteration approach, leveraging crowdsourced sampling across the CONUS. The data package associated with the CM study is available at https://data.ess-dive.lbl.gov/view/doi:10.15485/1923689. CM sampling began in April 2022 and ended in October 2023. This study uses subsamples from a subset of CM samples collected between June 2022 and June 2023. The original field samples were labeled as CM_###. Subsequent subsamples for this study were labeled as EC_###. The labels from the field samples and the EC subsamples can be mapped directly based on the digits following the prefix and underscore (i.e., EC_001 is a subsample from CM_001). See the critical details section below for more details on sample naming. This data package was originally published in August 2024. It was updated in February 2026 (v2; new and modified files). See the change history section in the readme for more details. For details on how to navigate this data package, see this infographic from the River Corridor SFA https://data.ess-dive.lbl.gov/portals/PNNLRiverCorridorSFA/About. This dataset is comprised of one folder of raw Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) data and one main data folder containing (1) file-level metadata; (2) data dictionary; (3) field metadata; (4) readme; (5) field protocol; and a (6) a subfolder with sediment sample data from the incubation experiment. The sample data subfolder contains (1) dissolved organic carbon (DOC, measured as non-purgeable organic carbon, NPOC); (2) total nitrogen (TN); (3) adenosine triphosphate (ATP); (4) percent carbon and nitrogen; (5) effect size; (6) iron (II); (7) gravimetric moisture; (8) respiration rates and raw dissolved oxygen values; (9) specific conductance; (10) pH; (11) temperature; (12) a summary containing median values of each data type for each treatment (wet and dry); (13) methods codes; (14) FTICR-MS methods; and (15) a subfolder of 9.4 Tesla FTICR-MS data. This folder contains three subfolders, one containing the sediment .xml data files, one containing the sediment CoreMS output files, the other containing instructions and scripts for processing the files in CoreMS (https://github.com/EMSL-Computing/CoreMS). All files are .csv, .pdf, .R, .ref, or .xml.

54 ENVIRONMENTAL SCIENCES↗

Leaf phenology data at The Morton Arboretum Forestry Plots 2019-2023

We are collecting long-term leaf phenology data at The Morton Arboretum to determine seasonal patterns of leaf production in trees. This data on leaf phenology will be integrated with other ongoing data streams to create a connection between above- and below-ground tree processes. This data package contains raw and smooth outputs from phenology data, as well as extracted phenophase dates (i.e., start, peak, and end of season): the raw and smooth outputs from the PhenoCam GUI can be found in the "leafRaw.csv" and "leafSmooth.csv" files, respectively, and the extracted phenophase dates can be found in the "leafPhenophaseDates2019-2023.csv" file. Extracted phenophase dates for evergreen species in 2023 are currently unavailable, and the files will be updated once they are extracted. Additional information on units and other file-level metadata can be found within each data file's respective data dictionary, and metadata for each of the 23 surveyed plots can be found within the "Location_metadata.csv" file. While the "leafRaw.csv" and the "leafSmooth.csv" files contain all data for all species, the "leafPhenophaseDates2019-2023.csv" file currently excludes the dates for evergreen species in 2023. Another version of the file will be added as those dates are extracted.

54 ENVIRONMENTAL SCIENCES↗

Data for Photosynthetic responses to temperature across the tropics: a meta-analytic approach

This data package contains raw data, data output, and code of photosynthetic temperature response curves from tropical forests. Data are used in "Photosynthetic responses to temperature across the tropics: a meta-analytic approach". The research investigates how photosynthetic optimum temperatures and shapes of photosynthetic temperature response curves varies across tropical forest climates and growth conditions. Growth climate variables include mean annual temperature, max and max temperature, diurnal temperature range, and aridity index. Meta-analysis combines 18 datasets with representation from Africa, Oceana, North American, and South America. Growth conditions analyzed considers deciduousness, successional status, light conditions, and whether plants are grown in situ or ex situ. All files, except for the raw datafile (Tropical_MetaAnalysis_Master_3.8.22_Edit.csv), have been processed using R code, which is provided.

54 ENVIRONMENTAL SCIENCES↗

High resolution characterization of soil dissolved organic matter with FTICR-MS (Fourier-transform ion cyclotron resonance mass spectrometry) from soil samples in control and warming plots in Blodgett Forest, CA (2014 and 2018)

The pathways of carbon transport and loss through and from soils—soil organic matter (SOM) depolymerization to dissolved organic carbon and mineralization to carbon dioxide (CO2)—are fundamentally driven by microbial activity, which is strongly regulated by environmental conditions. As part of Lawrence Berkeley National Laboratory (LBNL) Terrestrial Ecosystem Science (TES) Belowground Biogeochemistry Science Focus Area (SFA), we have established a novel whole-soil long-term warming experiment at the University of California (UC) Blodgett Forest Research Station (Sierra Nevada) in 2014, where we study the role of biogeochemical, microbial and geochemical process interactions in SOM decomposition and stabilization.This package contains Fourier transform ion cyclotron resonance mass spectrometry (21 Tesla FTICR-MS) data measured in negative and positive ionization mode from water and methanol soil extracts. Soil samples were collected in 2014/06/03 and 2018/06/04 from 3 replicated paired plots that had been subjected to experimental warming since June 2014 to simulate a predicted climate change scenario for northern California. The following files are included: (1) fticr_neg_h2oMeoh_data_raw.csv: raw data from combined water (H2O) and methanol (MeOH) extracts in negative ion mode, (2) fticr_neg_h2oMeoh_data_processed.csv: processed data from combined water (H2O) and methanol (MeOH) extracts in negative ion mode, (3) fticr_neg_metadata.csv: metadata for samples/measurements in negative ion mode, (4) fticr_pos_h2oMeoh_data_raw.csv: raw data from combined water (H2O) and methanol (MeOH) extracts in positive ion mode, (5) fticr_pos_h2oMeoh_data_processed.csv: processed data from combined water (H2O) and methanol (MeOH) extracts in positive ion mode, (6) fticr_pos_metadata.csv: metadata for samples/measurements in positive ion mode.

54 ENVIRONMENTAL SCIENCES↗

GNSS-based Vegetation Optical Depth, Tree Sway, and Evapotranspiration data from the Niwot Ridge Subalpine Forest (US-NR1) AmeriFlux site

This data package contains data and information about Global Navigation Satellite System (GNSS)-based Vegetation Optical Depth (VOD), tree sway motion, and eddy-covariance evapotranspiration (ET) data collected at the Niwot Ridge Subalpine Forest AmeriFlux site (US-NR1). The raw GNSS data were collected between May 2022 and August 2023. Other processed datasets such as tree sway motion and ET data are also included. The goal was to study the water content within a subalpine forest and, more specifically, examine the canopy evaporation process. This data archive includes all data that were used within the following Biogeosciences discussion paper that further summarizes the research objectives and conclusions:Burns, S.P., V. Humphrey, E.D. Gutmann, M.S. Raleigh, D.R. Bowling, and P.D. Blanken, 2025: Using GNSS-based vegetation optical depth, tree sway motion, and eddy-covariance to examine evaporation of canopy-intercepted rainfall in a subalpine forest. EGUsphere [preprint],https://doi.org/10.5194/egusphere-2025-1755This data archive also supplements the 30-min Lawrence Berkeley National Laboratory (LBNL) AmeriFlux dataset for US-NR1 (i.e., https://doi.org/10.17190/AMF/1246088) and updates what was in the 2020 ESS-DIVE US-NR1 archive (https://doi.org/10.15485/1671825) to include data from the years 2020-2025. More specifically, the following updates are provided: (i) five-minute statistics (means, variances, covariances) of all data measured by the US-NR1 data system between Sep 2020 and Jun 2025 in netCDF format, (ii) the electronic logbook of US-NR1 site visits, (iii) a web calendar (in HTML format) documenting activity at the site (a replica of https://urquell.colorado.edu/calendar/), (iv) photos taken at the site between years 2020 and present day (Aug 2025), and (v) several auxiliary datasets, primary related to trees near the site, soil properties, soil moisture and soil temperature, and subcanopy radiation data. The data package is setup so that the web calendar, photos, and electronic logbook can be easily accessed on a local computer using a web browser. The provided data files are in either BINEX or SBF format (for the raw GNSS data), netCDF, CSV, ASCII, or MATLAB format. To obtain a better understanding about the archive, please start by reading the following PDF which is included within the data archive:README_ESS_DIVE_USNR1_2025_readme_first.pdf.

54 ENVIRONMENTAL SCIENCES↗

Soil microbial ecology and microbiome-metabolite linkages improve understanding of ecosystem states along terrestrial-aquatic interfaces

These data are from Bandopadhyay et al., "Soil microbial ecology and microbiome-metabolite linkages improve understanding of ecosystem states along terrestrial-aquatic interfaces". This study aims to understand the soil microbial ecology along terrestrial-aquatic interfaces of a freshwater and estuarine region and how it relates to organic matter. We analyzed soil microbial (16S rRNA gene) and organic matter (Fourier-transform ion cyclotron resonance mass spectrometry, FTICR-MS) composition from upland (forested), transition (stressed forest), and wetland positions at three sites in each of the Lake Erie (freshwater) and Chesapeake Bay (estuarine) regions. This dataset includes 16S rRNA gene amplicon data (only processed file types included here) and organic matter composition from FTICR-MS data (raw and processed files included here) from upland (forested), transition (stressed forest), and wetland positions at three sites in each of the Lake Erie and Chesapeake Bay regions. These sites are part of the COMPASS-FME project (https://compass.pnnl.gov/FME/COMPASSFME). File formats and software needed to access files: 16S rRNA gene amplicon data: These files follow the format reported here https://ess-dive.gitbook.io/amplicon-sequencing-reporting-format#updates-in-v1.0.1. As per this format, there are four file types reported: 1. Taxon tables (also called sequence-by-sample or OTU (operational taxonomic unit)/ESV (exact sequence variant) tables) : available in a .txt file format and accessible using TextEdit or MS Excel. 2. Representative sequences (also called consensus sequences) : available in a .fasta format and accessible using TextEdit. 3. Sequencing metadata : available in a MS Excel workbook file format and CSV file format 4. Bioinformatic metadata : available in a MS Excel workbook file format and CSV file format FTICR-MS data: 1. Raw data converted to a processed file with intensities of the peaks in the given samples : available in a MS Excel CSV file format 2. Processed file used in analyses and visualizations (appended as icr_long_) : available in a MS Excel CSV file format 3. Metadata file for ICR features (appended as icr_meta) : available in a MS Excel CSV file format

54 ENVIRONMENTAL SCIENCES↗