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At least 739 records · Page 41
All sourcesNASA NTRSDOE OSTI
NASA NTRSDate not supplied

Studying Microbial Adaptation in the Laboratory: Sensor & Control Upgrades for an Experimental Evolution Biofluidics System

Experimental evolution (EE) involves iteratively exposing a microbial community to specific stressors to study its response to changes in environment over time. EE work is commonly done manually in the laboratory, but, when there are many environmental variables to measure and adjust, it is highly labor intensive, prone to human error, and challenging to scale. Single-purpose automated continuous culturing chambers exist, but implement only limited stressor types. A more general-purpose design is desirable. The BeING Lab at Ames Research Center created the prototype Automated Adaptive Directed Evolution Chamber (AADEC) to address these problems, beginning with Escherichia coli tolerance of short-wave ultraviolet (UV-C) radiation and of temperature. In newer versions, AADEC monitors microbial activity and can adjust the UV-C and temperature levels automatically. An optical density measurement is used to determine how many cells are present in the growth medium—over time, this corresponds to how many survive and reproduce. Oxidation-reduction potential provides information on consumed metabolic energy, and pH and electrical conductivity on metabolic products. Dissolved oxygen content is used to determine aerobic vs anaerobic growth. A Raspberry Pi computer processes all this data to set the UV-C stressor level. AADEC’s auxiliary systems include peristaltic pumps to change media and agitation to counteract cell settling. These actuators can also act as additional stressors. With the Raspberry Pi monitoring sensors and adjusting actuators in real time, AADEC takes measurements and controls the environment much more accurately than can be done with a manual EE implementation. The third and latest AADEC iteration is the first to simplify design and usage with circuits on PCBs and the ability to pre-program experimental protocols. Still planned is expansion to a multi-well design for the study of varying cell cultures in parallel, which will enable researchers to retain and re-inoculate cultures exhibiting the desired trait most strongly while flushing out others. AADEC’s special capabilities make it a valuable tool for studying life under multiple stressors, enabling scientists to replicate changes in climate on microbes for study in a lab setting.

Microbial Adaptation↗
NASA NTRS2020

LaRC SmartLab Apps For Instrument Control and Data Processing: Laboratory Environment Monitor

The LaRC SmartLab applications are a series of software tools to greatly enhance researcher efficiency by streamlining and automating workflows. Python scripts and applications are increasingly being used in scientific workflows, including for instrument control and data processing. Interactive Python scripting environments such as JupyterLab provide powerful tools for using Python. In some use cases, the development of standalone applications with dedicated graphical user interfaces can enhance the utility of the code and open it up to more users, including non-programmers. Here, we describe a Python based application for communicating with, and displaying data from, iTHX Temperature, Humidity, and Dew Point probes. We discuss the set up and use of the application as well as its implementation. We also highlight the use of Simulated probes to enable users and developers to familiarize with or debug the application, even when they do not have access to the physical hardware in the laboratory.

LaRC SmartLab↗
NASA NTRS2020

Analysis of 2018 Aerothermal Calibration Test at the NASA Propulsion Systems Laboratory

This report documents some of the key findings from an aerothermal calibration test that was conducted at the NASA Propulsion Systems Laboratory (PSL) as part of a series of fundamental ice-crystal icing tests in 2018. The purpose of the aerothermal calibration test was to characterize the cloud-off temperature and flow of the PSL test facility prior to performing the cloud-on icing experiments. This report presents the temperature measurements and analysis of that test day which examined uniformity and the influence of spray bars and wall temperature effects. Air temperature profiles were measured just upstream of the test section, and at the test section, for several cases across a range of parameters, including, but not limited to, spray-bar air temperature (Tnozz), altitude, air flow velocity, and spray-bar atomizing pressure (Pnozz).

NASA Propulsion Systems Laboratory↗
NASA NTRS2023

NASA Glenn Propulsion Systems Laboratory Customer Guide

This guide describes the Propulsion Systems Laboratory (PSL) at NASA Glenn Research Center (GRC) and was written to help customers utilizing the facility understand all the components of conducting a test program within the facility. The PSL complex supports two large engine test cells that simulate altitude flight conditions for a wide range of research and experimental tests. The test cells operate at altitudes up to 90,000 ft and speeds from subsonic to above supersonic. The way the facility sets test points at the engine or test article inlet, such as pressure, temperature, and Mach number is discussed. Support systems such as the heated and cooled combustion air system, the altitude exhaust system, the hydraulic system, the nitrogen, oxygen, and hydrogen systems, hydrogen burners, the engine exhaust gas-sampling system, and single- and multiple-axis thrust stands are addressed. Facility safety procedures, pre-test requirements, test operation standards are also stated in later sections. Past PSL tests and other capabilities are also discussed.

PSL Customer Guide↗
NASA NTRS2022

Characterization of Infrared Optical Motion Tracking System in NASA's Simulated Lunar Operations (SLOPE) Laboratory

This work characterizes the accuracy of a 16 camera OptiTrack motion tracking system installed in NASA Glenn Research Center's Simulated Lunar Operations (SLOPE) laboratory. The position of a rigid body mounted on a motorized linear stage is compared to its position reported by the motion tracking system as it travels through the facility's 777m$^3$ capture volume of interest. Experiments show that the mean error reported by the motion tracking system for the aggregate capture volume is in-line with independent measurements collected using the motion stage. Error within regions of the capture volume exceed the mean error reported by the motion tracking system, likely due to occlusion, and suggests that additional cameras should be used to increase measurement accuracy in these regions. Overall, results show that error values reported by the motion tracking system are representative of the measurement error in a collected data set and validates the system's use for characterizing the mobility and tractive performance of robots, rovers, and other vehicles for planetary exploration.

Motion capture↗
NASA NTRS2026

Glenn Research Center Propulsion Systems Laboratory 2026 Customer Guide

This guide describes the Propulsion Systems Laboratory (PSL) at the NASA Glenn Research Center. It was written to help customers understand the various components involved in conducting a test program within the PSL. The PSL complex supports two large-engine test cells that simulate altitude flight conditions for a wide range of research and experimental tests. These test cells operate at altitudes up to 90,000 ft and speeds from subsonic to above supersonic. Test points such as pressure, temperature, and Mach number can be set at the engine or test article inlet based on customer requirements. The facility’s support systems include the heated and cooled combustion air systems; altitude exhaust system; hydraulic system; nitrogen, oxygen, and hydrogen systems; thrust measurement system, which includes the facility’s single- and multi-axis thrust stands; inlet system; and electrical systems. In addition to providing a detailed description of PSL systems and capabilities, this guide discusses the facility’s history and past tests and addresses facility safety procedures, pretest requirements, and test operation standards.

Engine Icing Test Facility↗
NASA NTRS1962

Self erecting manned space laboratory

The Langley Research Center of the National Aeronautics and Space Administration is currently involved in several research programs on manned orbital space stations. This research effort is focused on seeking out and solving the problems which lie in the way of the eventual development of such a vehicle for use as a space laboratory. In conducting this research activity, it has been necessary to consider many vehicle configuration designs and operational concepts in some detail. The Langley Research Center space station study program has been in progress for several years, and it was consisted of both in-house activities and contracted efforts with industry. In the early Langley Research Center studies, only zero-gravity configurations were considered; however, the requirement for artificial gravity simulation for experimental purposes was soon added. Human factors considerations indicated that large diameter vehicles with slow rotational velocities generally permit the most comfortable living conditions for the crew. The configurations which were studied were required to be compatible with the planned launch vehicles such as Saturn, and manned spacecraft such as Mercury.

Manned Orbital Space Station (MOSS)↗
NASA NTRS1954

NACA Conference on Helicopters a Compilation of the Papers Presented at Langley Aeronautical Laboratory, Langley Field, Va., May 12 and 13, 1954

This document contains reproductions of technical papers presented at the NACA Conference on Helicopters held at the Langley Aeronautical Laboratory May 12 and 13, 1954. The primary purpose of this conference was to convey to the military services, their contractors, and others concerned with the design of helicopters the results of NACA research on helicopters and to provide those attending an opportunity to discuss the results. The papers in this document are in the same form in which they were presented at the conference in order to facilitate their prompt distribution. The original presentation and this record are considered as complementary to, rather than as substitutes for, the Committee's more complete and formal reports . A list of the conferees is included.

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