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DOE OSTI2024

Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting

Single-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), an integrated platform that enables global profiling of the transcriptome and proteome from same single cells via RNA sequencing and mass spectrometry-based proteomics, respectively. Benchmarking of nanoSPLITS demonstrates high measurement precision with deep proteomic and transcriptomic profiling of single-cells. We apply nanoSPLITS to cyclin-dependent kinase 1 inhibited cells and found phospho-signaling events could be quantified alongside global protein and mRNA measurements, providing insights into cell cycle regulation. We extend nanoSPLITS to primary cells isolated from human pancreatic islets, introducing an efficient approach for facile identification of unknown cell types and their protein markers by mapping transcriptomic data to existing large-scale single-cell RNA sequencing reference databases. Accordingly, we establish nanoSPLITS as a multiomic technology incorporating global proteomics and anticipate the approach will be critical to furthering our understanding of biological systems.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1984

An infrared alignment telescope

For problems involving the determination of the relative positions of optical components in a complex assembly, rather precise measurements can be routinely obtained when the visible portion of the spectrum is involved. Problems arise, however, when the lens elements to be aligned are made of infrared transmitting materials such as germanium, which is opaque to visible radiation. The alignment of a multichannel infrared measurement satellite called Haloe, which is currently being developed by NASA, represents an example of a particularly difficult task. The present paper is concerned with an infrared alignment telescope (IAT) which satisfies the need for a precision alignment tool capable of operating anywhere in the 2.4 to 12 micron wavelength range. When coupled to a commercial infrared scanner and display device, the IAT will provide a real-time picture of the relative positions of components and images over distances in the range from 20 inches to infinity.

Dilworth, D. C.↗

NASA NTRS1987

Middle atmosphere trace constituents; Proceedings of Workshop 9 of the Twenty-sixth COSPAR Plenary Meeting, Toulouse, France, June 30-July 11, 1986

Papers are presented on a reference model for stratospheric NO2, a proposed reference model for nitric acid, proposed reference models for ozone, and a background stratospheric aerosol reference model. Other topics include a comparison between the observed and calculated distributions of trace species in the middle atmosphere, recent measurements of trace constituents from rocket and balloon probes, NO2 field observations in the stratosphere during the MAP/GLOBUS Campaign in 1983 and 1985, and the vertical distribution of ozone in the equatorial zone. Also considered are precision measurements of stratospheric ozone profiles by rocket-borne optical ozonesondes, vertical profiles of source gases at tropical latitudes, and measurements of stratospheric trace constituent distributions from balloon-borne far infrared observations.

Keating, G. M.↗

NASA NTRS2008

The Photogrammetry Cube

We can determine distances between objects and points of interest in 3-D space to a useful degree of accuracy from a set of camera images by using multiple camera views and reference targets in the camera s field of view (FOV). The core of the software processing is based on the previously developed foreign-object debris vision trajectory software (see KSC Research and Technology 2004 Annual Report, pp. 2 5). The current version of this photogrammetry software includes the ability to calculate distances between any specified point pairs, the ability to process any number of reference targets and any number of camera images, user-friendly editing features, including zoom in/out, translate, and load/unload, routines to help mark reference points with a Find function, while comparing them with the reference point database file, and a comprehensive output report in HTML format. In this system, scene reference targets are replaced by a photogrammetry cube whose exterior surface contains multiple predetermined precision 2-D targets. Precise measurement of the cube s 2-D targets during the fabrication phase eliminates the need for measuring 3-D coordinates of reference target positions in the camera's FOV, using for example a survey theodolite or a Faroarm. Placing the 2-D targets on the cube s surface required the development of precise machining methods. In response, 2-D targets were embedded into the surface of the cube and then painted black for high contrast. A 12-inch collapsible cube was developed for room-size scenes. A 3-inch, solid, stainless-steel photogrammetry cube was also fabricated for photogrammetry analysis of small objects.

Source record↗

NASA NTRS2010

High Angular Resolution Observations of Episodic Dust Emission from Long Period Variable Stars Twenty Years of Observations with the Berkeley Infrared Spatial Interferometer

Over the past twenty years the U. C. Berkeley Infrared Spatial Interferometer has observed a number of Long Period Variable stars in the mid-infrared, obtaining information on the spatial distribution of dust around these stars with resolutions of the order of a few tens of milliarcseconds. The ISI is a heterodyne interferometer operating mostly at 11.15 microns, initially with two telescopes. In the last decade, it has been taking data regularly with three telescopes, thus obtaining visibility data on three baselines and also a closure phase. Over the course of the years, the ISI has been able to measure the physical properties of the dust shells surrounding these stars, in particular the inner radii of the dust shells, as well as the temperature and density distribution. For some stars, the ISI has also made precision measurements of their diameters in the mid-infrared. Closure phase measurements have revealed asymmetries in the dust distributions around many stars. Most surprisingly the ISI data has shown evidence for substantial changes in the amount of dust on time scales of 5-10 years, rather than being directly correlated with the stellar pulsation periods, which are of the order of one year. We discuss past results and new results from the ISI that highlight the dynamic environment around these stars.

Danchi, William↗

NASA NTRS2014

The Combustion Experiment on the Sample Analysis at Mars (SAM) Instrument Suite on the Curiosity Rover

Stern, J. C.↗

NASA NTRS2014

The Combustion Experiment on the Sample Analysis at Mars (SAM) Instrument Suite on the Curiosity Rover

The combustion experiment on the Sample Analysis at Mars (SAM) suite on Curiosity will heat a sample of Mars regolith in the presence of oxygen and measure composition of the evolved gases using quadrupole mass spectrometry (QMS) and tunable laser spectrometry (TLS). QMS will enable detection of combustion products such as CO, CO2, NO, and other oxidized species, while TLS will enable precise measurements of the abundance and carbon isotopic composition (delta(sup 13)C) of the evolved CO2 and hydrogen isotopic composition (deltaD) of H2O. SAM will perform a two-step combustion to isolate combustible materials below approx.550 C and above approx.550 C. The combustion experiment on SAM, if properly designed and executed, has the potential to answer multiple questions regarding the origins of volatiles seen thus far in SAM evolved gas analysis (EGA) on Mars. Constraints imposed by SAM and MSL time and power resources, as well as SAM consumables (oxygen gas), will limit the number of SAM combustion experiments, so it is imperative to design an experiment targeting the most pressing science questions. Low temperature combustion experiments will primarily target the quantification of carbon (and nitrogen) contributed by SAM wet chemistry reagants MTBSTFA (N-Methyl-N-tert-butyldimethylsilyltrifluoroacetamide) and DMF (Dimethylformamide), which have been identified in the background of blank and sample runs and may adsorb to the sample while the cup is in the Sample Manipulation System (SMS). In addition, differences between the sample and "blank" may yield information regarding abundance and delta(sup 13)C of bulk (both organic and inorganic) martian carbon. High temperature combustion experiments primarily aim to detect refractory organic matter, if present in Cumberland fines, as well as address the question of quantification and deltaD value of water evolution associated with hydroxyl hydrogen in clay minerals.

Mars↗

DOE OSTI2025

First Measurement of Charged-Current Muon-Neutrino-Induced 𝐾 + Production on Argon Using the MicroBooNE Detector

The MicroBooNE experiment is an 85 tonne active mass liquid argon time projection chamber neutrino detector exposed to the on-axis Booster Neutrino Beam at Fermilab. One of MicroBooNE’s physics goals is the precise measurement of neutrino interactions on argon in the 1 GeV energy regime. Building on the capabilities of the MicroBooNE detector, this analysis identifies 𝐾 + mesons, a key signature for the study of strange particle production in neutrino interactions. This measurement is furthermore valuable for background estimation for future nucleon decay searches and for improved reconstruction and particle identification capabilities in experiments such as the Deep Underground Neutrino Experiment. In this Letter, we present the first-ever measurement of a flux-integrated cross section for charged-current muon neutrino induced 𝐾 + production on argon nuclei, determined to be 7.93 ± 3.22⁢(stat) ± 2.83⁢(syst)×10 −42 cm 2 /nucleon based on an analysis of 6.88 × 10 20 protons on target. This result was found to be consistent with model predictions from different neutrino event generators within the reported uncertainties.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

DOE OSTI2023

Studies of the decay $$ {\textrm{D}}_{\textrm{s}}^{+}\to {\textrm{K}}^{+}{\textrm{K}}^{-}{\mu}^{+}{\nu}_{\mu } $$

Abstract The$$ {D}_s^{+}\to {K}^{+}{K}^{-}{\mu}^{+}{\nu}_{\mu } $$ D s + → K + K − μ + ν μ decay is studied based on 7.33 fb −1 ofe + e − collision data collected with the BESIII detector at center-of-mass energies in the range from 4.128 to 4.226 GeV. The absolute branching fraction is measured as$$ \mathcal{B}\left({D}_s^{+}\to \phi {\mu}^{+}{\nu}_{\mu}\right)=\left(2.25\pm 0.09\pm 0.07\right)\times {10}^{-2} $$ B D s + → ϕ μ + ν μ = 2.25 ± 0.09 ± 0.07 × 10 − 2 , the most precise measurement to date. Combining with the world average of$$ \mathcal{B}\left({D}_s^{+}\to \phi {e}^{+}{\nu}_e\right) $$ B D s + → ϕ e + ν e , the ratio of the branching fractions obtained is$$ \frac{\mathcal{B}\left({D}_s^{+}\to \phi {\mu}^{+}{\nu}_{\mu}\right)}{\mathcal{B}\left({D}_s^{+}\to \phi {e}^{+}{\nu}_e\right)}=0.94\pm 0.08 $$ B D s + → ϕ μ + ν μ B D s + → ϕ e + ν e = 0.94 ± 0.08 , in agreement with lepton universality. By performing a partial wave analysis, the hadronic form factor ratios atq 2 = 0 are extracted, finding$$ {r}_V=\frac{V(0)}{A_1(0)}=1.58\pm 0.17\pm 0.02 $$ r V = V 0 A 1 0 = 1.58 ± 0.17 ± 0.02 and$$ {r}_2=\frac{A_2(0)}{A_1(0)}=0.71\pm 0.14\pm 0.02 $$ r 2 = A 2 0 A 1 0 = 0.71 ± 0.14 ± 0.02 , where the first uncertainties are statistical and the second are systematic. No significantS-wave contribution fromf 0 (980)→ K + K − is found. The upper limit$$ \mathcal{B}\left({D}_s^{+}\to {f}_0(980){\mu}^{+}{\nu}_{\mu}\right)\cdot \mathcal{B}\left({f}_0(980)\to {K}^{+}{K}^{-}\right)<5.45\times {10}^{-4} $$ B D s + → f 0 980 μ + ν μ ⋅ B f 0 980 → K + K − < 5.45 × 10 − 4 is set at 90% credibility level.

Physics↗

DOE OSTI2023

Anharmonic phonon behavior via irreducible derivatives: Self-consistent perturbation theory and molecular dynamics

Cubic phonon interactions are now regularly computed from first principles, and the quartic interactions have begun to receive more attention. Given this realistic anharmonic vibrational Hamiltonian, the classical phonon Green's function can be precisely measured using molecular dynamics, which can then be used to rigorously assess the range of validity for self-consistent diagrammatic approaches in the classical limit. Here we use the bundled irreducible derivative approach to efficiently and precisely compute the cubic and quartic phonon interactions of CaF 2 , systematically obtaining the vibrational Hamiltonian purely in terms of irreducible derivatives. We demonstrate that the 4-phonon sunset diagram has an important contribution to the optical phonon linewidths beyond T = 500 K. Reasonable results are obtained even at T = 900 K when performing self-consistency using the 4-phonon loop diagram and evaluating the 3-phonon bubble and 4-phonon sunset diagrams post-self-consistency. Further improvements are obtained by performing quasiparticle perturbation theory, where both the 4-phonon loop and the real part of the 3-phonon bubble are employed during self-consistency. Finally, our irreducible derivative approach to self-consistent perturbation theory is a robust tool for studying anharmonic phonons in both the quantum and classical regimes.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

DOE OSTI2026

Measurement of 𝜋 0 production in $\bar{v}_μ$ charged-current interactions in the NOvA near detector

We present a high-statistics measurement of muon antineutrino-induced charged-current neutral pion production on a hydrocarbon target using the NOvA near detector. The differential cross sections as functions of the momenta and angles of the outgoing pion and muon, the squared four-momentum transfer, and the invariant mass of the hadronic system at an average neutrino energy of 2 GeV are measured and compared with predictions from various neutrino interaction models. The results agree with the GENIE prediction but suggest that other models underestimate the cross section in the Δ⁡(1232) resonance region. These results represent the most precise measurement of antineutrino-induced neutral pion production to date.

neutrino detection↗

NASA NTRS1976

Survey of quantitative data on the solar energy and its spectra distribution

This paper presents a survey of available quantitative data on the total and spectral solar irradiance at ground level and outside the atmosphere. Measurements from research aircraft have resulted in the currently accepted NASA/ASTM standards of the solar constant and zero air mass solar spectral irradiance. The intrinsic variability of solar energy output and programs currently under way for more precise measurements from spacecraft are discussed. Instrumentation for solar measurements and their reference radiation scales are examined. Insolation data available from the records of weather stations are reviewed for their applicability to solar energy conversion. Two alternate methods of solarimetry are briefly discussed.

Thekaekara, M. P.↗

NASA NTRS1993

The GPS Topex/Poseidon precise orbit determination experiment - Implications for design of GPS global networks

Topex/Poseidon, a cooperative satellite mission between United States and France, aims to determine global ocean circulation patterns and to study their influence on world climate through precise measurements of sea surface height above the geoid with an on-board altimeter. To achieve the mission science aims, a goal of 13-cm orbit altitude accuracy was set. Topex/Poseidon includes a Global Positioning System (GPS) precise orbit determination (POD) system that has now demonstrated altitude accuracy better than 5 cm. The GPS POD system includes an on-board GPS receiver and a 6-station GPS global tracking network. This paper reviews early GPS results and discusses multi-mission capabilities available from a future enhanced global GPS network, which would provide ground-based geodetic and atmospheric calibrations needed for NASA deep space missions while also supplying tracking data for future low Earth orbiters. Benefits of the enhanced global GPS network include lower operations costs for deep space tracking and many scientific and societal benefits from the low Earth orbiter missions, including improved understanding of ocean circulation, ocean-weather interactions, the El Nino effect, the Earth thermal balance, and weather forecasting.

Lindqwister, Ulf J.↗

NASA NTRSDate not supplied

Development of Optical Step Height Measurement Capability for the BOLT Flight Vehicle

This paper presents details on the development of testing procedures to evaluate two different optical step height sensors that were intended for use on the first and second Boundary Layer Transition (BOLT and BOLT II) flight vehicle as well as other flight vehicles. The accurate and precise measurement of step height at the interface of different sections of a flight vehicle is important, as small changes in the step height (in some instances < 10 microns) at such locations can have a significant influence on the development of a boundary layer over the outer surface of the vehicle, which in turn can significantly impact aeroheating (among other things). In this paper, testing procedures have been developed, and test hardware assembled, to determine the capabilities of two optical step height sensor candidates. The first candidate is a miniaturized camera sensor that would be embedded into the body of a flight vehicle and would image the interface between two sections of that vehicle. The second candidate is a small fiber-based optical sensor that would also be embedded into the body of the flight vehicle, but projects light onto the interface between two sections of the flight vehicle, and then monitors any reflected/scattered light from that interface. Preliminary data from these two sensor candidates are presented, and a discussion of improvements that will be made in the testing procedures and to the test hardware is provided.

Brett F Bathel↗

NASA NTRS1993

A stepped-frequency Delta-K microwave radar for oceanographic studies

A stepped-frequency Delta-K (SFDK) radar was developed to remotely sense ocean surface characteristics. This C-band radar uses frequency diversity and real-time signal processing for improved Delta-K measurements. In this paper, we show how frequency diversity can be implemented to enhance the energy of the resonant peak of the cross-product spectrum relative to the background energy. This feature is essential for making real-time measurements of resonant peak frequency over extended time periods. The SFDK was used in a month-long field experiment at N. Truro (Massachusetts). The results presented show that the phase velocity of ocean surface waves could be precisely measured 87 percent of the time.

Popstefanija, Ivan↗

NASA NTRS2016

Development of Hydroxyl Tagging Velocimetry for Low Velocity Flows

Hydroxyl tagging velocimetry (HTV) is a molecular tagging technique that relies on the photo-dissociation of water vapor into OH radicals and their subsequent tracking using laser induced fluorescence. Velocities are then obtained from time-of-flight calculations. At ambient temperature in air, the OH species lifetime is relatively short (<50 μs), making it suited for high speed flows. Lifetime and radicals formation increases with temperature, which allows HTV to also probe low-velocity, high-temperature flows or reacting flows such as flames. The present work aims at extending the domain of applicability of HTV, particularly towards low-speed (<10 m/s) and moderate (<500 K) temperature flows. Results are compared to particle image velocimetry (PIV) measurements recorded in identical conditions. Single shot and averaged velocity profiles are obtained in an air jet at room temperature. By modestly raising the temperature (100-200 degC) the OH production increases, resulting in an improvement of the signal-to-noise ratio (SNR). Use of nitrogen - a non-reactive gas with minimal collisional quenching - extends the OH species lifetime (to over 500 μs), which allows probing of slower flows or, alternately, increases the measurement precision at the expense of spatial resolution. Instantaneous velocity profiles are resolved in a 100degC nitrogen jet (maximum jet-center velocity of 6.5 m/s) with an uncertainty down to 0.10 m/s (1.5%) at 68% confidence level. MTV measurements are compared with particle image velocimetry and show agreement within 2%.

Andre, Matthieu A.↗

DOE OSTI2025

A New Measurement of the Neutron Electric Form Factor with the Super Bigbite Spectrometer Apparatus

Protons and neutrons, collectively known as nucleons, make up the nuclei at the core of atoms which form our world. The nucleon has been under intensive study for over 100 years, and yet we still do not fully understand the internal dynamics which govern properties like its spin or its mass-which contributes to almost all of the visible mass in the universe. These dynamics are governed by quantum chromodynamics(QCD), the predictions of which are experimentally tested at high energy accelerator facilities such as Jefferson Lab. TheGEN-II experiment (E12-09-016) is one such experiment. GEN-II is part of the Super Bigbite Spectrometer (SBS) experimental form factor pro gramme taking place in Hall A at Jefferson Lab, which aims to make precision measurements of the nucleon electromagnetic form factors(EMFFs)at record high values of squared four momentum transfer ¿2. EMFFs describe the electric and magnetic moment distributions within the nucleon. They can be measured through elastic electron scattering off the nucleon, and describe the recoil response of the target nucleon at a given energy scale. GEN-II is a double polarized semi-exclusive beam target asymmetry (BTA) experiment, seeking to measure the electric form factor of the neutron,¿¿ ¿,at three new values of squared four-momentum transfer¿2 =2.92,6.74and9.82GeV². The latter two points being at record high ¿2. The form factor is determined through measuring the BTA of quasielliptical scattering of a neutron from a polarized nuclear target. The experiment utilized the CEBAF accelerator to produce longitudinally polarized electrons up to ~85% polarization, which were scattered off neutrons within a novel polarized helium-3 (³He) target. This new polarized ³He target was employed by building on the technology of its precursors which existed in similar preceding experiments. This target was designed to operate at the high luminosities typical of Hall A, and reached a record breaking combination of polarization and beam intensity known as figure of merit, three times larger than those predecessors. The SBS collaboration designed and constructed two brand new high acceptance spectrometers for these experiments, an electron arm named Bigbite (BB) and a hadron arm named Super Bigbite. Both spectrometers featured a large acceptance EM dipole magnet, and complementary detector systems. The electron arm contained gaseous electronmultipliers(GEMs) which were used for high precision tracking of the scattered electrons, a heavy gas cherenkov (GRINCH) which was used for PID between electrons and pions, a plastic i ii scintillator timing hodoscope to provide high resolution timing of the start of events, and a pair of EM calorimeters (BBCal) which provided energy measurements of detected particles, and provided the experimental trigger. The hadron arm also contained a system of GEMs which will be utilized for future SBS experiments, and a hadron calorimeter designed to provide position, timing and energy measurements of the recoiling nucleon. The calibration of all detector subsystems, beam and target data is discussed, with a focus on novel timing calibrations to the hodoscope and hadron calorimeter. An analysis of selecting quasielliptical events and suppressing background contributions from a number of sources which contaminate the final event sample is given. The largest irreducible backgrounds are found to be from misidentified protons, timing accidentals and inelastic events. The physical asymmetry is measured and used to extract a value for the form factor ratio ¿¿ ¿/¿¿ ¿. High precision ¿2 data for ¿¿ ¿ is used to then extract ¿¿ ¿. This work finds at ¿2 = 2.92 GeV2 that ¿¿ ¿ = 0.0129+0.0019 -0.0020. This result is in statistical agreement with existing fits to world data, and predictions from the constituent quark model and Dyson–Schwinger equations, in this region of ¿2.

Penman, Gary [Univ. of Glasgow, Scotland (United K↗

DOE OSTI2023

The Covariance of PFNS Results from the Chi-Nu Experiment

The prompt fission neutron spectrum (PFNS) from neutron-induced fission is a fundamental quantity for the behavior of nuclear reactors, and has been measured many times on a wide variety of nuclei and covering different ranges of incident and emitted neutron energies. However, results from past measurements are frequently called into question in modern nuclear data evaluations because of a lack of thorough experimental documentation and incomplete uncertainty analyses. The Chi-Nu experiment at Los Alamos National Laboratory was designed to produce high-precision measurements of the PFNS of major actinides over a wide range of incident and emitted neutron energies, and with the documentation and covariance analysis required to ensure that the results of this experiment maintain their impact long into the future, thereby avoiding this pitfall of past measurements. In this work we describe the Chi-Nu experiment along with summaries of the treatment of and methods developed to address two important components of the analysis of Chi-Nu data: random-coincidence backgrounds and MCNP simulations. Furthermore, we describe the first results for correlations not just between all data points collected on a single target nucleus, but also between all data points from separate Chi-Nu measurements on 235 U and 239 Pu. These correlations are important for accurately calculating ratios of the PFNS from one actinide to another, which are rare and can be informative for nuclear data evaluation efforts.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗