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All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Development of an Unmanned Mobile Current Turbine Platform: Preprint

The design and development of a prototype unmanned mobile floating platform, equipped with a custom low-flow marine current turbine for autonomously seeking and harnessing tidal/coastal currents is described. The platform is an unmanned surface vehicle in the form of a catamaran with two electric outboard motors and capabilities for autonomous navigation. An undershot water wheel, aided by a custom flow concentrator, has been selected as the basic design for the marine current turbine, which is mounted on the stern of the unmanned surface vehicle platform. The concept of operation is that the platform would navigate to a designated marine current resource, autonomously anchor at the location, align itself in the current and deploy the current turbine using a custom cable-lift deployment mechanism. As the turbine harnesses the local current, an onboard power-take-off device converts the harnessed mechanical energy to electricity which is stored in onboard batteries. Considerations of deployment in tidal and coastal waters required obtaining the necessary environmental permits for conducting in-water testing; developing required mitigation measures in protecting local wildlife and their habitats; and identifying potential in-water test sites and surveying them for their suitability in terms of the current resource, bottom type, water depth, and local boat traffic. The design and development of the turbine and the results of initial in-water testing are discussed. The potential for scaling up the system for extended capacity is presented.

ENGINEERING,TIDAL AND WAVE POWER↗
NASA NTRS1997

Rocket Engine Numerical Simulator (RENS)

Work is being done at three universities to help today's NASA engineers use the knowledge and experience of their Apolloera predecessors in designing liquid rocket engines. Ground-breaking work is being done in important subject areas to create a prototype of the most important functions for the Rocket Engine Numerical Simulator (RENS). The goal of RENS is to develop an interactive, realtime application that engineers can utilize for comprehensive preliminary propulsion system design functions. RENS will employ computer science and artificial intelligence research in knowledge acquisition, computer code parallelization and objectification, expert system architecture design, and object-oriented programming. In 1995, a 3year grant from the NASA Lewis Research Center was awarded to Dr. Douglas Moreman and Dr. John Dyer of Southern University at Baton Rouge, Louisiana, to begin acquiring knowledge in liquid rocket propulsion systems. Resources of the University of West Florida in Pensacola were enlisted to begin the process of enlisting knowledge from senior NASA engineers who are recognized experts in liquid rocket engine propulsion systems. Dr. John Coffey of the University of West Florida is utilizing his expertise in interviewing and concept mapping techniques to encode, classify, and integrate information obtained through personal interviews. The expertise extracted from the NASA engineers has been put into concept maps with supporting textual, audio, graphic, and video material. A fundamental concept map was delivered by the end of the first year of work and the development of maps containing increasing amounts of information is continuing. Find out more information about this work at the Southern University/University of West Florida. In 1996, the Southern University/University of West Florida team conducted a 4day group interview with a panel of five experts to discuss failures of the RL10 rocket engine in conjunction with the Centaur launch vehicle. The discussion was recorded on video and audio tape. Transcriptions of the entire proceedings and an abbreviated video presentation of the discussion highlights are under development. Also in 1996, two additional 3year grants were awarded to conduct parallel efforts that would complement the work being done by Southern University and the University of West Florida. Dr. Prem Bhalla of Jackson State University in Jackson, Mississippi, is developing the architectural framework for RENS. By employing the Rose Rational language and Booch Object Oriented Programming (OOP) technology, Dr. Bhalla is developing the basic structure of RENS by identifying and encoding propulsion system components, their individual characteristics, and cross-functionality and dependencies. Dr. Ruknet Cezzar of Hampton University, located in Hampton, Virginia, began working on the parallelization and objectification of rocket engine analysis and design codes. Dr. Cezzar will use the Turbo C++ OOP language to translate important liquid rocket engine computer codes from FORTRAN and permit their inclusion into the RENS framework being developed at Jackson State University. The Southern University/University of West Florida grant was extended by 1 year to coordinate the conclusion of all three efforts in 1999.

Davidian, Kenneth O.↗
NASA NTRS2010

Endangered and Threatened Species at Kennedy Space Center Merritt Island National Wildlife Refuge

Throughout my internship, I assisted with the long-term monitoring of the Florida Scrub- Jay (Aphelocoma coerulescens), a threatened species endemic to Florida. The Florida Scrub Jay diet consists of insects and small vertebrates throughout most of the year; however, during the winter their primary diet is acorns because the insect population is low. Furthermore, the Florida Scrub-Jay is a habitat specialist that lives in a disappearing plant community called the scrub, which consists of sand live oak, myrtle oak and chapman oak. The Florida Scrub-Jay is considered threatened because its numbers are decreasing primarily due to the loss of habitat that it needs to survive. Scrub habitat is highly desirable for human development because it is high, dry, and sandy. Periodic controlled burns maintain the scrub in a low, open condition favored by Scrub-Jays. Florida Scrub-Jays build their nests approximately 3-5 feet (approximately 1.5 m) above the ground in shrubby oaks (Breininger 153), mate for life and are cooperative breeders; which means that the young jays remain in their natal territory for at least a year to help their parents defend their territory, feed the young, and mob predators. (Breininger 152). I assisted in conducting monthly censuses at long-term monitoring sites and a juvenile in July survey to determine reproductive success for the year. In addition, to Scrub-Jay monitoring, I also had the opportunity to assist with some long term monitoring of ecosystem recovery. Scrub is a fire maintained system. Fire maintains the structure of scrub necessary for many of the threatened species that reside in the scrub habitat.

Galdolfi, Catherine↗
NASA NTRS2007

Weather Research and Forecasting Model Sensitivity Comparisons for Warm Season Convective Initiation

Mesoscale weather conditions can significantly affect the space launch and landing operations at Kennedy Space Center (KSC) and Cape Canaveral Air Force Station (CCAFS). During the summer months, land-sea interactions that occur across KSC and CCAFS lead to the formation of a sea breeze, which can then spawn deep convection. These convective processes often last 60 minutes or less and pose a significant challenge to the forecasters at the National Weather Service (NWS) Spaceflight Meteorology Group (SMG). The main challenge is that a "GO" forecast for thunderstorms and precipitation is required at the 90 minute deorbit decision for End Of Mission (EOM) and at the 30 minute Return To Launch Site (RTLS) decision at the Shuttle Landing Facility. Convective initiation, timing, and mode also present a forecast challenge for the NWS in Melbourne, FL (MLB). The NWS MLB issues such tactical forecast information as Terminal Aerodrome Forecasts (TAFs), Spot Forecasts for fire weather and hazardous materials incident support, and severe/hazardous weather Watches, Warnings, and Advisories. Lastly, these forecasting challenges can also affect the 45th Weather Squadron (45 WS), which provides comprehensive weather forecasts for shuttle launch, as well as ground operations, at KSC and CCAFS. The need for accurate mesoscale model forecasts to aid in their decision making is crucial. Both the SMG and the MLB are currently implementing the Weather Research and Forecasting Environmental Modeling System (WRF EMS) software into their operations. The WRF EMS software allows users to employ both dynamical cores - the Advanced Research WRF (ARW) and the Non-hydrostatic Mesoscale Model (NMM). There are also data assimilation analysis packages available for the initialization of the WRF model- the Local Analysis and Prediction System (LAPS) and the Advanced Regional Prediction System (ARPS) Data Analysis System (ADAS). Having a series of initialization options and WRF cores, as well as many options within each core, provides SMG and NWS MLB with a lot of flexibility. It also creates challenges, such as determining which configuration options are best to address specific forecast concerns. The goal of this project is to assess the different configurations available and to determine which configuration will best predict warm season convective initiation in East-Central Florida. Four different combinations of WRF initializations will be run (ADAS-ARW, ADAS-NMM, LAPS-ARW, and LAPS-NMM) at a 4-km resolution over the Florida peninsula and adjacent coastal waters. Five candidate convective initiation days using three different flow regimes over East-Central Florida will be examined, as well as two null cases (non-convection days). Each model run will be integrated 12 hours with three runs per day, at 0900, 1200, and 1500 UTe. ADAS analyses will be generated every 30 minutes using Level II Weather Surveillance Radar-1988 Doppler (WSR-88D) data from all Florida radars to verify the convection forecast. These analyses will be run on the same domain as the four model configurations. To quantify model performance, model output will be subjectively compared to the ADAS analyses of convection to determine forecast accuracy. In addition, a subjective comparison of the performance of the ARW using a high-resolution local grid with 2-way nesting, I-way nesting, and no nesting will be made for select convective initiation cases. The inner grid will cover the East-Central Florida region at a resolution of 1.33 km. The authors will summarize the relative skill of the various WRF configurations and how each configuration behaves relative to the others, as well as determine the best model configuration for predicting warm season convective initiation over East-Central Florida.

Watson, Leela R.↗
DOE OSTI2015

Measuring organic matter in Everglades wetlands and the Everglades Agricultural Area

Here, organic matter is a complex material that represents the long-term decay products from plants and other organisms in the soil. When organic matter is allowed to build up in a soil, the soil color at the surface usually turns a darker color, often with a red or brown hue. Typically in Florida mineral soils, organic matter content is quite low, within the range of 1 to 5%. However, in some soils that remain flooded for most of the year, organic matter can build up with time and actually become the soil. Such is the case for the organic soils, or histosols, found in southern Florida. These organic soils comprise much of the Water Conservation Areas, Everglades National Park (ENP), Big Cypress Basin, and the Everglades Agricultural Area (EAA). It is important to document organic matter accumulation in the Everglades to gauge the effectiveness of wetland creation and succession. For the EAA, the drained soils lose organic matter due to oxidation, so measurement of the organic matter content of these soils over the course of time indicates the oxidation potential and mineral incorporation from bedrock. Due to the wide diversity of soil types and methods of measuring soil organic matter, there is a need to devise a more universal method applicable to many types of histosols in south Florida. The intent of this publication is: 1.To describe a simple laboratory method for determining the organic matter content of the organic soils of southern Florida and demonstrate the importance of using this new procedure for improved accuracy and precision; 2.To utilize this updated laboratory procedure for field sites across Everglades wetlands and the EAA; and 3. To recommend this procedure be used by growers, state and federal agencies, and university and agency researchers dealing with the management of organic soils in southern Florida. Growers can use this improvement to organic matter measurement to keep lab testing costs low while getting a better, more quantitative estimate of organic carbon (organic matter) for decisions regarding pesticide applications and estimated contribution of nutrients released from the organic matter in their fields. Restoration efforts in the Everglades wetlands can be better documented with the lower cost, but now equally as useful, LOI test for organic carbon. Improvements to soil organic matter coupled with other measurements of biological health of the system can be documented with less work using the adjusted LOI calculations.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Rationale for reconsidering current regulations restricting use of hybrids in orange juice

Huanglongbing (HLB) is a disease that has devastated the Florida citrus industry, threatens the entire U.S. citrus industry, and globally is rapidly spreading. Florida’s citrus production is 90% sweet orange, which is quite sensitive to HLB. The heavy reliance on sweet orange for Florida citrus production makes the industry especially vulnerable to diseases that are damaging to this type of citrus. Furthermore, 90% of Florida oranges are used in producing orange juice that is defined by a federal regulation known as the “orange juice standard”, specifying that at least 90% of “orange juice” must be derived from Citrus sinensis. Genomic analyses definitively reveal that sweet orange is not a true species, but just one of many introgression hybrids of C. reticulata and C. maxima, with phenotypic diversity resulting from accumulated mutations in this single hybrid, the “sweet orange”. No other fruit industry is limited by law to such a narrow genetic base. Fortunately, there are new citrus hybrids displaying reduced sensitivity to HLB, and in some cases they produce juice, alone or in blends, that consumers would recognize as “orange juice”. Reconsidering current regulations on orange juice standards may permit use of such hybrids in “orange juice”, providing greater latitude for commercialization of these hybrids, leading to higher-quality orange juice and a more sustainable Florida orange juice industry.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2016

Achieving Challenge Home in Affordable Housing in the Hot - Humid Climate

The Building America Partnership for Improved Residential Construction (BA-PIRC), one of the Building America research team leads, has partnered with two builders as they work through the Challenge Home certification process in one test home each. The builder partners participating in this cost-shared research are Southeast Volusia County Habitat for Humanity near Daytona, Florida and Manatee County Habitat for Humanity near Tampa, Florida. Both are affiliates of Habitat for Humanity International, a non-profit affordable housing organization. This research serves to identify viable technical pathways to meeting the CH criteria for other builders in the region. A further objective of this research is to identify gaps and barriers in the marketplace related to product availability, labor force capability, code issues, cost effectiveness, and business case issues that hinder or prevent broader adoption on a production scale. The builder partners participating in this cost-shared research are Southeast Volusia County Habitat for Humanity (SEVHFH) near Daytona, Florida and Manatee County Habitat for Humanity (MCHFH) near Tampa, Florida. Both are affiliates of Habitat for Humanity International (HFHI), a nonprofit affordable housing organization. Manatee Co Habitat for Humanity Challenge home - Ellenton, FL 34222 S. Sarasota Challenge Home - Sarasota, FL Southeast Volusia co Habitat for Humanity's Challenge Home - Edgewater, FL 32141 Several iterations of home, Challenge Home as built, Challenge Home without solar, and Challenge Home configured to just meet Florida Code. All houses redone in BEOpt 2.1.0.2

affordable housing↗
DOE OSTI2019

Blue crab larval dispersal highlights population connectivity and implications for fishery management

In the Western Atlantic and Gulf of Mexico (GOM), the blue crab Callinectes sapidus fishery is managed at regional scale and its assessment does not consider the population structure of the species. To understand connectivity of the blue crab population, we simulated larval dispersal using a biophysical model driven by high-resolution ocean currents and including early life-history traits of the species. Simulations were conducted during 2015 and 2016, and larvae were released from locations along Florida’s GOM and Atlantic coasts. A high degree of local larval retention was observed in Florida’s GOM waters, mainly during summer when weak southeastern winds tend to yield a shoreward net flow. Results demonstrated clear evidence of connectivity between the Gulf coast of Florida population and those of Mississippi, Alabama, and Louisiana, suggesting that the blue crab populations in the GOM are intermixed and the hypothesized boundary (in Florida) of two stocks needs further consideration. Outputs of the model also indicated connectivity between the blue crab populations of Florida’s Gulf coast and the South-Atlantic Bight (SAB). Larval trajectories show inter-annual variability driven by the interaction of winds, Loop Current (LC) intrusions in the northern GOM, LC eddies and their cyclonic counterparts, and the Mississippi River plume. The latter provides a conduit for larval transport from the GOM to the SAB. These findings provide evidence of the physical oceanographic processes that sustain the homogenous genetic population structure for blue crabs between SAB and GOM populations, highlighting the need for collaborative management in US waters.

Criales, Maria M.↗
NASA NTRS1999

Radar Observations of Convective Systems from a High-Altitude Aircraft

Reflectivity data collected by the precipitation radar on board the tropical Rainfall Measuring Mission (TRMM) satellite, orbiting at 350 km altitude, are compared to reflectivity data collected nearly simultaneously by a doppler radar aboard the NASA ER-2 flying at 19-20 km altitude, i.e. above even the deepest convection. The TRMM precipitation radar is a scanning device with a ground swath width of 215 km, and has a resolution of about a4.4 km in the horizontal and 250 m in the vertical (125 m in the core swath 48 km wide). The TRMM radar has a wavelength of 217 cm (13.8 GHz) and the Nadir mirror echo below the surface is used to correct reflectivity for loss by attenuation. The ER-2 Doppler radar (EDOP) has two antennas, one pointing to the nadir, 34 degrees forward. The forward pointing beam receives both the normal and the cross-polarized echos, so the linear polarization ratio field can be monitored. EDOP has a wavelength of 3.12 cm (9.6 GHz), a vertical resolution of 37.5 m and a horizontal along-track resolution of about 100 m. The 2-D along track airflow field can be synthesized from the radial velocities of both beams, if a reflectivity-based hydrometer fall speed relation can be assumed. It is primarily the superb vertical resolution that distinguishes EDOP from other ground-based or airborne radars. Two experiments were conducted during 1998 into validate TRMM reflectivity data over convection and convectively-generated stratiform precipitation regions. The Teflun-A (TEXAS-Florida Underflight) experiment, was conducted in April and May and focused on mesoscale convective systems mainly in southeast Texas. TEFLUN-B was conducted in August-September in central Florida, in coordination with CAMEX-3 (Convection and Moisture Experiment). The latter was focused on hurricanes, especially during landfall, whereas TEFLUN-B concentrated on central; Florida convection, which is largely driven and organized by surface heating and ensuing sea breeze circulations. Both TEFLUN-A and B were amply supported by surface data, in particular a dense raingauge network, a polarization radar, wind profilers, a mobile radiosonde system, a cloud physics aircraft penetrating the overflown storms, and a network of 10 cm Doppler radars(WSR-88D). This presentation will show some preliminary comparisons between TRMM, EDOP, and WSR-88D reflectivity fields in the case of an MCS, a hurricane, and less organized convection in central Florida. A validation of TRMM reflectivity is important, because TRMM's primary objective is to estimate the rainfall climatology with 35 degrees of the equator. Rainfall is estimated from the radar reflectivity, as well from TRMM's Microwave Imager, which measures at 10.7, 19.4, 21.3, 37, and 85.5 GHz over a broader swath (78 km). While the experiments lasted about three months the cumulative period of near simultaneous observations of storms by ground-based, airborne and space borne radars is only about an hour long. Therefore the comparison is case-study-based, not climatological. We will highlight fundamental differences in the typical reflectivity profiles in stratiform regions of MCS's, Florida convection and hurricanes and will explain why Z-R relationships based on ground-based radar data for convective systems over land should be different from those for hurricanes. These catastrophically intense rainfall from hurricane Georges in Hispaniola and from Mitch in Honduras highlights the importance of accurate Z-R relationships, It will be shown that a Z-R relationship that uses the entire reflectivity profile (rather than just a 1 level) works much better in a variety of cases, making an adjustment of the constants for different precipitation system categories redundant.

Heymsfield, G.↗
NASA NTRS2014

Tech Transfer News

On October 28, 2011, the White House released a Presidential Memorandum entitled: Accelerating Technology Transfer and Commercialization of Federal Research in Support of High-Growth Businesses. With this memo, the President challenged all federal agencies conducting R&D to accelerate technology transfer and commercialization of federally developed technology to help stimulate the national economy. The NASA Technology Transfer Program responded by asking the center technology transfer offices to reach out to - and work more closely with - their regional economic development organizations to promote the transfer of NASA technologies to the local private sector for use in the marketplace. Toward that effort, the KSC Technology Transfer Office teamed with the Florida Space Coast Economic Development Commission (EDC) to host a technology transfer forum designed to increase our business community's awareness of available KSC technologies for transfer. In addition, the forum provided opportunities for commercial businesses to collaborate with KSC in technology development. (see article on page 12) The forum, held on September 12, 2013, focused on KSC technology transfer and partnership opportunities within the Robotics, Sustainability, Information Technology and Environmental Remediation technology areas. The event was well attended with over 120 business leaders from the community. KSC Center Director Robert Cabana and the Center Chief Technologist Karen Thompson provided remarks, and several KSC lead researchers presented technical information and answered questions, which were not in short supply. Florida Today and the Orlando Sentinel ran news stories on the forum and both NASA TV and Channel 6 News filmed portions of the event. Given the reaction by the media and local business to the forum, it is evident the community is recognizing the opportunities that NASA-developed technologies can provide to aspiring entrepreneurs and existing companies to bring new technologies to market, as well as the positive impact KSC technology transfer can have on the local economy. We see even more evidence of this in the efforts by several other organizations to develop programs that provide aspiring entrepreneurs with the opportunity and training needed to identify the commercial potential of specific NASA technologies and develop business plans to exploit that potential. Several initiatives include Florida Startup Quest, CareerSource Brevard Energy Launch, Rollins College Entrepreneurial Scholar of Distinction Program, and a new effort led by the University of Central Florida Office of Research and Commercialization to stimulate new business growth in Florida based on NASA technologies. The KSC Technology Transfer Office has stepped up to support each of these programs and is providing them with the NASA technologies they need to help move the economy forward.

News↗
NASA NTRS2021

Large-scale space use of large juvenile and adult smalltooth sawfish Pristis pectinata: implications for management

The smalltooth sawfish Pristis pectinata is an endangered species endemic to the Atlantic Ocean. The only known viable populations occur in the USA along both coasts of Florida and in the western Bahamas. Little is known about habitat use and movement ecology of large juvenile and adult smalltooth sawfish. Although Critical Habitat — a management designation in the USA — has been identified for small juveniles, it has yet to be identified for these life stages. Between May 2016 and April 2019, we used passive acoustic telemetry and 3 large data sharing networks of receivers to track movements of 43 large juvenile and adult smalltooth sawfish. During this study, 24 females and 19 males were implanted with transmitters with estimated 4 or 10 yr battery lives. These tagged individuals were detected off the southeastern USA on 461 receivers ranging from off the coast of Brunswick, Georgia, to the lower Florida Keys, and along the Gulf coast to Apalachee Bay, Florida. Seasonal migrations were undertaken by 58% (43% mature; 57% immature) of the tagged individuals, with the remainder being apparent residents of their tagging locations. Tagged sawfish from both size classes and of both sexes migrated, which indicates that neither sex nor length is a predictor of whether a sawfish will migrate or not. Although both coasts of Florida were used for migration, most individuals consistently used the same coast when they migrated. The areas surrounding Boca Grande, Cape Canaveral, and the lower Florida Keys were heavily visited sites that could be further evaluated as potential Critical Habitat for these life stages. Understanding the movement patterns of this Critically Endangered species is essential for creating policies to protect areas important for promoting growth of the population.

Pristis pectinata Habitat use · Acoustic monitorin↗
NASA NTRS2015

Toward Connecting Subtropical Algal Blooms to Freshwater Nutrient Sources Using a Long-Term, Spatially Distributed, In Situ Chlorophyll-A Record

Harmful algal blooms are increasing in tropical estuaries which can have complex morphologies and hydrologic regimes while being less well studied than temperate estuaries. Spatial and temporal patterns of algal bloom occurrence in Florida Bay were examined to evaluate the potential contribution of the various freshwater inputs to the subtropical bay as nutrient sources. Monthly water quality data, from 1989 to 2009, at 28 sampling stations across the bay were analyzed at the station-month level, aggregated into hydrologic Zones of Similar Influence and based on annual rainfall seasons. The Zones of Similar Influence are linked to the geomorphology of the bay with western areas being more directly connected to the Southwest Florida Shelf waters than eastern areas. Correlation analysis suggested that inputs of phosphorus were the predominant factor in the initiation of elevated chlorophyll a (chl-a) levels but was also consistent with higher nitrogen limitation in western Florida Bay as reported in literature. Differences in mean monthly chl-a indicated a seasonality of algal blooms with elevated chl-a concentrations following heavy precipitation months for stations in the north-central and western areas of the bay where algal blooms have been re-occurring. Differences in stations' chl-a concentrations showed stations to the northwest as having significantly higher concentrations than more interior stations during the dry season but not during the rainy season (when algal blooms are occurring). Mapping the sampling stations atop the bathymetry of Florida Bay highlighted the importance of coastal morphology in evaluation of potential nutrient pathways for estuarine algal bloom sources. The specific factors resulting in the seasonal cycles of blooms remained unresolved but portions of the bay and times of year were identified as important areas for further research. This study indicates that illustrating the interplay of geomorphology and winds and rains at fine temporal and spatial resolution is required to describe nutrient circulation for systems with complex morphologies such as those associated with reefs, island matrices and headlands.

Florida Bay↗
DOE OSTI2013

2013 DOE Bioenergy Technologies Office (BETO) Project Peer Review—Biodiesel Cellulosic Ethanol Research Project (Hendry County Sustainable Biofuels Center)

The presentation provides an overview of the Biodiesel Cellulosic Ethanol Research Project (Hendry County Sustainable Biofuels Center). It summarizes the project history, timeline, budget, partners, objectives, goals, future plans and in closer detail reviews the used approaches and technical accomplishments. The main project goals were (1) developing strategies and tools that assist in the creation of economically and environmentally sustainable bioenergy industries within ecologically-sensitive regions such as South Florida and, in particular, the greater Everglades, (2) using these bioenergy strategies and tools in evolving the existing agricultural, urban, and ecological sectors towards more sustainable structures and practices and (3) using bioenergy as a focal point in the larger effort to mitigate climate change and sea level rise, realities with particularly catastrophic consequences for South Florida. The project started on Oct 1, 2010 and ended on Feb 28, 2013. It yearly average budget was $369,770, with the Dept. of Energy annual cost share of $317,167. The main project partners were Hendry County, University of Florida - Institute of Food and Agricultural Sciences, Intelligentsia International, Inc., Edison State College and University of South Florida. Used approaches, main accomplishments and results in the categories of (1) technical research, (2) education and (3) business development are presented in detail. The project uniqueness is mainly related to the use of system approaches and integrating several systems analyses. Relevance of the project applicable to sustainability of bioenergy, food production, & restoration is explained, critical success factors are challenges are outlined and future work drafted. Finally, the main publications and presentations catalogue list is presented.

09 BIOMASS FUELS↗
DOE OSTI2019

Assessing the Ability of Zonal δ 18 O Contrast in Benthic Foraminifera to Reconstruct Deglacial Evolution of Atlantic Meridional Overturning Circulation

δ 18 O in foraminifera δ 18 O c ) is a useful proxy for density, and the strength of the Atlantic Meridional Overturning Circulation (AMOC) can be reconstructed by the zonal density contrast in the Atlantic. However, whether the deglacial zonal δ 18 O c contrast can represent the AMOC change is still unclear. δ 18 O c contrast across the Florida Straits has been hypothesized as a proxy for the AMOC evolution during the last deglaciation, but the strength of Florida Current could also be influenced by wind forcing. In this work, we examine the ability of the zonal δ 18 O c contrast to reconstruct AMOC in a deglacial model simulation. The model simulation suggests that the deglacial variation of the Florida Current strength is dominated by AMOC, with the wind effect on the variation of the Florida Current being negligible. Furthermore, the δ 18 O c contrast across the western boundary along the entire Atlantic and the basin-wide δ 18 O c contrast in the North Atlantic in the upper ocean can also be used to reconstruct AMOC. However, using basin-wide δ 18 O c contrast to reconstruct AMOC in the South Atlantic is not possible at all water depths. In the subtropical South Atlantic, the basin-wide δ 18 O c contrast is decoupled from the density contrast between 400 to 600 m through the deglaciation because of the deglacial change of the Antarctic Intermediate Water. Thus, δ 18 O c is a useful proxy to reconstruct past density and in turn past AMOC, but caution has to be used when using the basin-wide δ 18 O c contrast to reconstruct the basin-wide density contrast in the South Atlantic.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2014

The Transcriptome of an Amphioxus, Asymmetron lucayanum, from the Bahamas: A Window into Chordate Evolution

Cephalochordates, the sister group of tunicates plus vertebrates, have been called “living fossils” due to their resemblance to fossil chordates from Cambrian strata. The genome of the cephalochordate Branchiostoma floridae shares remarkable synteny with vertebrates and is free from whole-genome duplication. We performed RNA sequencing from larvae and adults of Asymmetron lucayanum, a cephalochordate distantly related to B. floridae. Comparisons of about 430 orthologous gene groups among both cephalochordates and 10 vertebrates using an echinoderm, a hemichordate, and a mollusk as outgroups showed that cephalochordates are evolving more slowly than the slowest evolving vertebrate known (the elephant shark), with A. lucayanum evolving even more slowly than B. floridae. Against this background of slow evolution, some genes, notably several involved in innate immunity, stand out as evolving relatively quickly. This may be due to the lack of an adaptive immune system and the relatively high levels of bacteria in the inshore waters cephalochordates inhabit. Molecular dating analysis including several time constraints revealed a divergence time of ~120 Ma for A. lucayanum and B. floridae. The divisions between cephalochordates and vertebrates, and that between chordates and the hemichordate plus echinoderm clade likely occurred before the Cambrian.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Age-specific case data reveal varying dengue transmission intensity in US states and territories

Dengue viruses (DENV) are endemic in the US territories of Puerto Rico, American Samoa, and the US Virgin Islands, with focal outbreaks also reported in the states of Florida and Hawaii. However, little is known about the intensity of dengue virus transmission over time and how dengue viruses have shaped the level of immunity in these populations, despite the importance of understanding how and why levels of immunity against dengue may change over time. These changes need to be considered when responding to future outbreaks and enacting dengue management strategies, such as guiding vaccine deployment. We used catalytic models fitted to case surveillance data stratified by age from the ArboNET national arboviral surveillance system to reconstruct the history of recent dengue virus transmission in Puerto Rico, American Samoa, US Virgin Islands, Florida, Hawaii, and Guam. We estimated average annual transmission intensity (i.e., force of infection) of DENV between 2010 and 2019 and the level of seroprevalence by age group in each population. We compared models and found that assuming all reported cases are secondary infections generally fit the surveillance data better than assuming all cases are primary infections. Using the secondary case model, we found that force of infection was highly heterogeneous between jurisdictions and over time within jurisdictions, ranging from 0.00008 (95% CrI: 0.00002–0.0004) in Florida to 0.08 (95% CrI: 0.044–0.14) in American Samoa during the 2010–2019 period. For early 2020, we estimated that seropositivity in 10 year-olds ranged from 0.09% (0.02%–0.54%) in Florida to 56.3% (43.7%–69.3%) in American Samoa. In the absence of serological data, age-specific case notification data collected through routine surveillance combined with mathematical modeling are powerful tools to monitor arbovirus circulation, estimate the level of population immunity, and design dengue management strategies.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2017

α-Copaene is an attractant, synergistic with quercivorol, for improved detection of Euwallacea nr. fornicatus (Coleoptera: Curculionidae: Scolytinae)

The tea shot-hole borer, Euwallacea fornicatus Eichhoff, is an ambrosia beetle endemic to Asia and a pest of commercial tea, Camellia sinensis (L.) Kuntze. Recently, a complex of species morphologically similar to E. fornicatus has been recognized, which includes new pests established in Israel and the USA, both in California and Florida. Collectively termed E. nr. fornicatus, these cryptic species carry symbiotic Fusarium spp. fungi, some of which cause dieback disease in susceptible hosts, which include avocado, Persea americana Miller. Due to the threat to this economically important crop, research was initiated to evaluate efficacy of kairomone-based lures for detection of the beetle in Florida (termed the Florida tea shot hole borer, FL-TSHB). A series of field tests were conducted in 2016 in commercial avocado groves known to have FL-TSHB at various population levels. All tests evaluated lures containing quercivorol (p-menth-2-en-1-ol) andα-copaene, presented separately and in combination; and one test evaluated effect of trap type on beetle captures. In addition, electroantennography (EAG) was used to quantify female olfactory responses to lure emissions. This study identified (-)-α-copaene as a new attractant for FL-TSHB, equivalent in efficacy to quercivorol (the standard lure for Euwallacea detection in the USA); however, the combination of lures captured significantly more FL-TSHB than either lure alone. This combination resulted in synergistic attraction at two field sites and additive attraction at a third site. Sticky panel traps captured more FL-TSHB than comparably-baited Lindgren funnel traps. Females engaged in host-seeking flight from 11:00 to 16:00 hr (EST), with peak numbers observed between 12:00 and 13:00 hr. EAG analyses confirmed olfactory chemoreception of both kairomones, with a higher response elicited with the combination of volatiles. Results indicate that detection of pest E. nr. fornicatus in Florida can be improved by using a two-component lure consisting of p-menth-2-en-1-ol and (-)-α-copaene.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2017

Comparative Performance of Two Ventilation Strategies in a Hot-Humid Climate

In fiscal year 2013, Pacific Northwest National Laboratory (PNNL), Florida Solar Energy Center (FSEC), and Florida Home Energy and Resources Organization (Florida HERO) began a collaborative effort to evaluate the impact of ventilation rate on interior comfort conditions, space-conditioning energy use, and indoor air contaminant concentrations. Relevant parameters were measured in 10 homes in Gainesville, Florida, along with corresponding outdoor conditions, to characterize the impact of differing ventilation rates. This report provides information about the data collection method and results from more than 1 year of data collection during a period from summer 2013 through summer 2014. Indoor air quality was sampled in three discrete periods with the first occurring in August/September 2013, the second occurring in March/April 2014, and the third occurring in August 2014.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗