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At least 739 records · Page 41

Studying Microbial Adaptation in the Laboratory: Sensor & Control Upgrades for an Experimental Evolution Biofluidics System

Experimental evolution (EE) involves iteratively exposing a microbial community to specific stressors to study its response to changes in environment over time. EE work is commonly done manually in the laboratory, but, when there are many environmental variables to measure and adjust, it is highly labor intensive, prone to human error, and challenging to scale. Single-purpose automated continuous culturing chambers exist, but implement only limited stressor types. A more general-purpose design is desirable. The BeING Lab at Ames Research Center created the prototype Automated Adaptive Directed Evolution Chamber (AADEC) to address these problems, beginning with Escherichia coli tolerance of short-wave ultraviolet (UV-C) radiation and of temperature. In newer versions, AADEC monitors microbial activity and can adjust the UV-C and temperature levels automatically. An optical density measurement is used to determine how many cells are present in the growth medium—over time, this corresponds to how many survive and reproduce. Oxidation-reduction potential provides information on consumed metabolic energy, and pH and electrical conductivity on metabolic products. Dissolved oxygen content is used to determine aerobic vs anaerobic growth. A Raspberry Pi computer processes all this data to set the UV-C stressor level. AADEC’s auxiliary systems include peristaltic pumps to change media and agitation to counteract cell settling. These actuators can also act as additional stressors. With the Raspberry Pi monitoring sensors and adjusting actuators in real time, AADEC takes measurements and controls the environment much more accurately than can be done with a manual EE implementation. The third and latest AADEC iteration is the first to simplify design and usage with circuits on PCBs and the ability to pre-program experimental protocols. Still planned is expansion to a multi-well design for the study of varying cell cultures in parallel, which will enable researchers to retain and re-inoculate cultures exhibiting the desired trait most strongly while flushing out others. AADEC’s special capabilities make it a valuable tool for studying life under multiple stressors, enabling scientists to replicate changes in climate on microbes for study in a lab setting.

Microbial Adaptation↗

Controlling Gas-Flow Mass Ratios

Proposed system automatically controls proportions of gases flowing in supply lines. Conceived for control of oxidizer-to-fuel ratio in new gaseous-propellant rocket engines. Gas-flow control system measures temperatures and pressures at various points. From data, calculates control voltages for electronic pressure regulators for oxygen and hydrogen. System includes commercially available components. Applicable to control of mass ratios in such gaseous industrial processes as chemical-vapor depostion of semiconductor materials and in automotive engines operating on compressed natural gas.

Morris, Brian G.↗

TLSLEAF: Automatic Leaf Angle Estimates From Single-Scan Terrestrial Laser Scanning

Leaf angle distribution (LAD) in forest canopies affects estimates of leaf area, light interception, and global-scale photosynthesis, but is often simplified to a single theoretical value. Here, we present TLSLeAF (Terrestrial Laser Scanning Leaf Angle Function), an automated open-source method of deriving LADs from terrestrial laser scanning. TLSLeAF produces canopy-scale leaf angle and LADs by relying on gridded laser scanning data. The approach increases processing speed, improves angle estimates, and requires minimal user input. Key features are automation, leaf–wood classification, beta parameter output, and implementation in R to increase accessibility for the ecology community. TLSLeAF precisely estimates leaf angle with minimal distance effects on angular estimates while rapidly producing LADs on a consumer-grade machine. We challenge the popular spherical LAD assumption, showing sensitivity to ecosystem type in plant area index and foliage profile estimates that translate to c. 25% and c. 11% increases in canopy net photosynthesis (c. 25%) and solar-induced chlorophyll fluorescence (c. 11%). TLSLeAF can now be applied to the vast catalog of laser scanning data already available from ecosystems around the globe. The ease of use will enable widespread adoption of the method outside of remote-sensing experts, allowing greater accessibility for addressing ecological hypotheses and large-scale ecosystem modeling efforts.

3D↗

Shape optimization of three-dimensional stamped and solid automotive components

The shape optimization of realistic, 3-D automotive components is discussed. The integration of the major parts of the total process: modeling, mesh generation, finite element and sensitivity analysis, and optimization are stressed. Stamped components and solid components are treated separately. For stamped parts a highly automated capability was developed. The problem description is based upon a parameterized boundary design element concept for the definition of the geometry. Automatic triangulation and adaptive mesh refinement are used to provide an automated analysis capability which requires only boundary data and takes into account sensitivity of the solution accuracy to boundary shape. For solid components a general extension of the 2-D boundary design element concept has not been achieved. In this case, the parameterized surface shape is provided using a generic modeling concept based upon isoparametric mapping patches which also serves as the mesh generator. Emphasis is placed upon the coupling of optimization with a commercially available finite element program. To do this it is necessary to modularize the program architecture and obtain shape design sensitivities using the material derivative approach so that only boundary solution data is needed.

Botkin, M. E.↗

A systems engineering approach to automated failure cause diagnosis in space power systems

Automatic failure-cause diagnosis is a key element in autonomous operation of space power systems such as Space Station's. A rule-based diagnostic system has been developed for determining the cause of degraded performance. The knowledge required for such diagnosis is elicited from the system engineering process by using traditional failure analysis techniques. Symptoms, failures, causes, and detector information are represented with structured data; and diagnostic procedural knowledge is represented with rules. Detected symptoms instantiate failure modes and possible causes consistent with currently held beliefs about the likelihood of the cause. A diagnosis concludes with an explanation of the observed symptoms in terms of a chain of possible causes and subcauses.

Dolce, James L.↗

LEADER - An integrated engine behavior and design analyses based real-time fault diagnostic expert system for Space Shuttle Main Engine (SSME)

The LEADER expert system has been developed for automatic learning tasks encompassing real-time detection, identification, verification, and correction of anomalous propulsion system operations, using a set of sensors to monitor engine component performance to ascertain anomalies in engine dynamics and behavior. Two diagnostic approaches are embodied in LEADER's architecture: (1) learning and identifying engine behavior patterns to generate novel hypotheses about possible abnormalities, and (2) the direction of engine sensor data processing to perform resoning based on engine design and functional knowledge, as well as the principles of the relevant mechanics and physics.

Gupta, U. K.↗

Automating hypertext for decision support

A decision support system (DSS) shell is being constructed that can support applications in a variety of fields, e.g., engineering, manufacturing, finance. The shell provides a hypertext-style interface for 'navigating' among DSS application models, data, and reports. The traditional notion of hypertext had to be enhanced. Hypertext normally requires manually, pre-defined links. A DSS shell, however, requires that hypertext connections to be built 'on the fly'. The role of hypertext is discussed in augmenting DSS applications and the decision making process. Also discussed is how hypertext nodes, links, and link markers tailored to an arbitrary DSS application were automatically generated.

Bieber, Michael↗

Fail-over file transfer process

The present invention provides a fail-over file transfer process to handle data file transfer when the transfer is unsuccessful in order to avoid unnecessary network congestion and enhance reliability in an automated data file transfer system. If a file cannot be delivered after attempting to send the file to a receiver up to a preset number of times, and the receiver has indicated the availability of other backup receiving locations, then the file delivery is automatically attempted to one of the backup receiving locations up to the preset number of times. Failure of the file transfer to one of the backup receiving locations results in a failure notification being sent to the receiver, and the receiver may retrieve the file from the location indicated in the failure notification when ready.

Semancik, Susan K.↗

Pattern Recognition for a Flight Dynamics Monte Carlo Simulation

The design, analysis, and verification and validation of a spacecraft relies heavily on Monte Carlo simulations. Modern computational techniques are able to generate large amounts of Monte Carlo data but flight dynamics engineers lack the time and resources to analyze it all. The growing amounts of data combined with the diminished available time of engineers motivates the need to automate the analysis process. Pattern recognition algorithms are an innovative way of analyzing flight dynamics data efficiently. They can search large data sets for specific patterns and highlight critical variables so analysts can focus their analysis efforts. This work combines a few tractable pattern recognition algorithms with basic flight dynamics concepts to build a practical analysis tool for Monte Carlo simulations. Current results show that this tool can quickly and automatically identify individual design parameters, and most importantly, specific combinations of parameters that should be avoided in order to prevent specific system failures. The current version uses a kernel density estimation algorithm and a sequential feature selection algorithm combined with a k-nearest neighbor classifier to find and rank important design parameters. This provides an increased level of confidence in the analysis and saves a significant amount of time.

Restrepo, Carolina↗

Software Aids Visualization of Computed Unsteady Flow

Unsteady Flow Analysis Toolkit (UFAT) is a computer program that synthesizes motions of time-dependent flows represented by very large sets of data generated in computational fluid dynamics simulations. Prior to the development of UFAT, it was necessary to rely on static, single-snapshot depictions of time-dependent flows generated by flow-visualization software designed for steady flows. Whereas it typically takes weeks to analyze the results of a largescale unsteady-flow simulation by use of steady-flow visualization software, the analysis time is reduced to hours when UFAT is used. UFAT can be used to generate graphical objects of flow visualization results using multi-block curvilinear grids in the format of a previously developed NASA data-visualization program, PLOT3D. These graphical objects can be rendered using FAST, another popular flow visualization software developed at NASA. Flow-visualization techniques that can be exploited by use of UFAT include time-dependent tracking of particles, detection of vortex cores, extractions of stream ribbons and surfaces, and tetrahedral decomposition for optimal particle tracking. Unique computational features of UFAT include capabilities for automatic (batch) processing, restart, memory mapping, and parallel processing. These capabilities significantly reduce analysis time and storage requirements, relative to those of prior flow-visualization software. UFAT can be executed on a variety of supercomputers.

Kao, David↗

Model Generation to Support Model-Based Testing Applied on NASA DAT - An Experience Report

Model-based Testing (MBT), where a model of the system under tests (SUT) behavior is used to automatically generate executable test cases, is a promising and versatile testing technology. Nevertheless, adoption of MBT technologies in industry is slow and many testing tasks are performed via manually created executable test cases (i.e. test programs such as JUnit). In order to adopt MBT, testers must learn how to construct models and use these models to generate test cases, which might be a hurdle. An interesting observation in our previous work is that the existing manually created test cases often provided invaluable insights for the manual creation of the testing models of the system. In this paper we present an approach that allows the tester to first create and debug a set of test cases. When the tester is happy with the test cases, the next step is to automatically generate a model from the test cases. The generated model is derived from the test cases, which are actions that the system can perform (e.g. a button clicks) and their expected outputs in form of assert statements (e.g. assert data entered). The model is a Finite State Machine (FSM) model that can be employed with little or no manual changes to generate additional test cases for the SUT. We successfully applied the approach in a feasibility study to the NASA Data Access Toolkit (DAT), which is a web-based GUI. One compelling finding is that the test cases that were generated from the automatically generated models were able to detect issues that were not detected by the original set of manually created test cases. We present the findings from the case study and discuss best practices for incorporating model generation techniques into an existing testing process.

State Machines↗

Calibration Results for J-ERS-1 SAR Data Produced by the Alaska SAR Facility

The Alaska SAR Facility has been receiving and processing SAR data from the J-ERS-1 satellite since Spring 1992. Corner reflectors have been set up for J-ERS-1 SAR calibration at a site near Delta Junction, in central Alaska. Image quality and calibration analysis results from the Delta Junction site and others will be presented in this paper. The impact of the 3-bit Analog-to-Digital Converter and the automatic stepping of the gain as a function of range in the J-ERS-1 radar receiver on calibration performance has been assessed. Preliminary observations on J-ERS-1 SAR data are that the average Signal-to-Noise ratio is generally fairly low, in the range 5-6dB. Azimuth ambiguity levels are higher than preflight analysis indicated. Over land, the dynamic range in the backscatter at L-band for approximately 36 degree incidence angle is often fairly high.

Freeman, A.↗

Calibration Results for J-ERS-1 SAR Data Produced by the Alaska SAR Facility

The Alaska SAR Facility has been receiving and processing SAR data from the J-ERS-1 satellite since Spring 1992. Corner reflectors have been set up for J-ERS-1 SAR calibration at a site near Delta Junction, in central Alaska. Image quality and calibration analysis results from the Delta Junction site and others will be presented in this paper. The impact of the 3-bit Analog-to-Digital Converter and the automatic stepping of the gain as a function of range in the J-ERS-1 radar receiver on calibration performance has been assessed. Preliminary observations on J-ERS-1 SAR data are that the average Signal-to-Noise ratio is generally fairly low, in the range 5-6 dB. Azimuth ambiguity levels are higher than preflight analysis indicated. Over land, the dynamic range in the backscatter at L-band for approximately 36 degree incidence angle is often fairly high...

Freeman, A.↗

Sequence History Update Tool

The Sequence History Update Tool performs Web-based sequence statistics archiving for Mars Reconnaissance Orbiter (MRO). Using a single UNIX command, the software takes advantage of sequencing conventions to automatically extract the needed statistics from multiple files. This information is then used to populate a PHP database, which is then seamlessly formatted into a dynamic Web page. This tool replaces a previous tedious and error-prone process of manually editing HTML code to construct a Web-based table. Because the tool manages all of the statistics gathering and file delivery to and from multiple data sources spread across multiple servers, there is also a considerable time and effort savings. With the use of The Sequence History Update Tool what previously took minutes is now done in less than 30 seconds, and now provides a more accurate archival record of the sequence commanding for MRO.

Khanampompan, Teerapat↗

Master/Programmable-Slave Computer

Unique modular computer features compactness, low power, mass storage of data, multiprocessing, and choice of various input/output modes. Master processor communicates with user via usual keyboard and video display terminal. Coordinates operations of as many as 24 slave processors, each dedicated to different experiment. Each slave circuit card includes slave microprocessor and assortment of input/output circuits for communication with external equipment, with master processor, and with other slave processors. Adaptable to industrial process control with selectable degrees of automatic control, automatic and/or manual monitoring, and manual intervention.

Smaistrla, David↗

Analysis of the GPS Observations of the Site Survey at Sheshan 25-m Radio Telescope in August 2008

The processing of the GPS observations of the site survey at Sheshan 25-m radio telescope in August 2008 is reported. Because each session in this survey is only about six hours, not allowing the subdaily high frequency variations in the station coordinates to be reasonably smoothed, and because there are serious cycle slips in the observations and a large volume of data would be rejected during the software automatic adjustment of slips, the ordinary solution settings of GAMIT needed to be adjusted by loosening the constraints in the a priori coordinates to 10 m, adopting the "quick" mode in the solution iteration, and combining Cview manual operation with GAMIT automatic fixing of cycle slips. The resulting coordinates of the local control polygon in ITRF2005 are then compared with conventional geodetic results. Due to large rotations and translations in the two sets of coordinates (geocentric versus quasi-topocentric), the seven transformation parameters cannot be solved for directly. With various trial solutions it is shown that with a partial pre-removal of the large parameters, high precision transformation parameters can be obtained with post-fit residuals at the millimeter level. This analysis is necessary to prepare the follow-on site and transformation survey of the VLBI and SLR telescopes at Sheshan

Liu, L.↗

SUPIN: A Tool for the Aerodynamic Design and Analysis of Supersonic Inlets

The Supersonic Inlet Design and Analysis Tool (SUPIN) is a computational program that performs geometric modeling and aerodynamic design and analysis of a set of supersonic inlets whose types include the axisymmetric pitot, three-dimensional pitot, axisymmetric, two-dimensional, two-dimensional bifurcated-duct, and streamline-traced inlets. The inlets are modeled by dividing the inlet flowpath into components that start at the freestream and ends at the engine face. The primary components of the inlet include the external supersonic diffuser, throat section, and subsonic diffuser. Each component is characterized by a set of inputs that include geometric and aerodynamic factors. Examples of the geometric factors are angles, lengths, area ratios, and coordinates that describe the geometry of the components. Examples of the aerodynamic factors are Mach numbers, flow ratios, and pressure ratios that set constraints or desired aerodynamic properties of the component. These input factors are specified within a text-based input data file that is read into SUPIN. SUPIN is a Fortran 95 program that performs the inlet design and analysis as a batch or non-interactive process. SUPIN writes output data files describing the inlet geometry and aerodynamic performance. SUPIN uses analytic, empirical, and numerical methods to perform the inlet design and analysis. The inlet geometry is constructed using planar and three-dimensional geometry constructs and is represented by coordinates, angles, areas, profiles, and surfaces. Surfaces are output in the form of a Plot3D surface grid file. SUPIN does have some capability to automatically generate planar and three-dimensional, multi-block, structured grids for computational fluid dynamics (CFD) analysis. The aerodynamic analysis performed by SUPIN computes quasi-one-dimensional properties, such as Mach numbers, pressures, and temperatures, through the inlet flowpath. Planar aerodynamic properties, such as those for shock and Mach waves, are computed for the supersonic compression of the external supersonic diffuser and throat section. The inlet performance is characterized by the inlet flow rates, total pressure recovery, and inlet drag. The primary intent of this document is to describe the usage of SUPIN. The methods used within SUPIN are documented and may serve as a useful reference for understanding the aerodynamic design and analysis of supersonic inlets.

supersonic inlets↗

Path planning for planetary rover using extended elevation map

This paper describes a path planning method for planetary rovers to search for paths on planetary surfaces. The planetary rover is required to travel safely over a long distance for many days over unfamiliar terrain. Hence it is very important how planetary rovers process sensory information in order to understand the planetary environment and to make decisions based on that information. As a new data structure for informational mapping, an extended elevation map (EEM) has been introduced, which includes the effect of the size of the rover. The proposed path planning can be conducted in such a way as if the rover were a point while the size of the rover is automatically taken into account. The validity of the proposed methods is verified by computer simulations.

Nakatani, Ichiro↗