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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 721 records · Page 40
All sourcesNASA NTRSDOE OSTI
DOE OSTI2018

Cyclic di-AMP Acts as an Extracellular Signal That Impacts Bacillus subtilis Biofilm Formation and Plant Attachment

ABSTRACT There is a growing appreciation for the impact that bacteria have on higher organisms. Plant roots often harbor beneficial microbes, such as the Gram-positive rhizobacterium Bacillus subtilis , that influence their growth and susceptibility to disease. The ability to form surface-attached microbial communities called biofilms is crucial for the ability of B. subtilis to adhere to and protect plant roots. In this study, strains harboring deletions of the B. subtilis genes known to synthesize and degrade the second messenger cyclic di-adenylate monophosphate (c-di-AMP) were examined for their involvement in biofilm formation and plant attachment. We found that intracellular production of c-di-AMP impacts colony biofilm architecture, biofilm gene expression, and plant attachment in B. subtilis . We also show that B. subtilis secretes c-di-AMP and that putative c-di-AMP transporters impact biofilm formation and plant root colonization. Taken together, our data describe a new role for c-di-AMP as a chemical signal that affects important cellular processes in the environmentally and agriculturally important soil bacterium B. subtilis . These results suggest that the “intracellular” signaling molecule c-di-AMP may also play a previously unappreciated role in interbacterial cell-cell communication within plant microbiomes. IMPORTANCE Plants harbor bacterial communities on their roots that can significantly impact their growth and pathogen resistance. In most cases, however, the signals that mediate host-microbe and microbe-microbe interactions within these communities are unknown. A detailed understanding of these interaction mechanisms could facilitate the manipulation of these communities for agricultural or environmental purposes. Bacillus subtilis is a plant-growth-promoting bacterium that adheres to roots by forming biofilms. We therefore began by exploring signals that might impact its biofilm formation. We found that B. subtilis secretes c-di-AMP and that the ability to produce, degrade, or transport cyclic di-adenylate monophosphate (c-di-AMP; a common bacterial second messenger) affects B. subtilis biofilm gene expression and plant attachment. To our knowledge, this is the first demonstration of c-di-AMP impacting a mutualist host-microbe association and suggests that c-di-AMP may function as a previously unappreciated extracellular signal able to mediate interactions within plant microbiomes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2018

An Experimental Study of the Influence of an External Signal on the Generation Mode of a Megawatt-Power Gyrotron

The frequency capture of radiation by an external signal was experimentally studied for the first time for a megawatt-power gyrotron. An external signal from the magnetron at a frequency of 35 GHz entered the working space of the gyrotron through a synthesized two-mirror quasi-optical converter that allowed transforming the input signal into a working mode. This converter has been recently developed and implemented in the Institute of Applied Physics, Russian Academy of Sciences. The radiation spectra were experimentally obtained in modes of frequency capture and frequency beats. The dependence of the frequency capture bandwidth on the power of the external signal was found. Comparison of experimental results with the data of numerical modelling of gyrotron equations with an external signal showed their good agreement.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2022

Effect of the pump beam profile and wavefront on the amplified signal wavefront in optical parametric amplifiers

We present a theoretical and experimental analysis of the signal phase introduced by the pump-beam wavefront and spatial profile during optical parametric amplification (OPA) process. The theory predicts the appearance of an additional wavefront in the amplified signal beam that is proportional to the spatial derivative of the pump-beam wavefront. The effect of the pump-beam profile on the signal-beam wavefront is also investigated. Our experiments tested these theoretical predictions by comparing the wavefront of the signal beam before and after amplification in a multi-joule broadband OPA. The measured signal wavefront was shown to have the expected dependence on the pump-beam profile and wavefront. These results can be considered when designing petawatt-scale ultrabroadband optical parametric chirped-pulse–amplification systems.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2014

Phosphorylation Site Dynamics of Early T-cell Receptor Signaling

In adaptive immune responses, T-cell receptor (TCR) signaling impacts multiple cellular processes and results in T-cell differentiation, proliferation, and cytokine production. Although individual protein–protein interactions and phosphorylation events have been studied extensively, we lack a systems-level understanding of how these components cooperate to control signaling dynamics, especially during the crucial first seconds of stimulation. Here, we used quantitative proteomics to characterize reshaping of the T-cell phosphoproteome in response to TCR/CD28 co-stimulation, and found that diverse dynamic patterns emerge within seconds. We detected phosphorylation dynamics as early as 5 s and observed widespread regulation of key TCR signaling proteins by 30 s. Development of a computational model pointed to the presence of novel regulatory mechanisms controlling phosphorylation of sites with central roles in TCR signaling. The model was used to generate predictions suggesting unexpected roles for the phosphatase PTPN6 (SHP-1) and shortcut recruitment of the actin regulator WAS. Predictions were validated experimentally. This integration of proteomics and modeling illustrates a novel, generalizable framework for solidifying quantitative understanding of a signaling network and for elucidating missing links.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2016

Modulation of phytochrome signaling networks for improved biomass accumulation using a bioenergy crop model

Plant growth and development, including stem elongation, flowering time, and shade-avoidance habits, are affected by wavelength composition (i.e., light quality) of the light environment. the molecular mechanisms underlying light perception and signaling pathways in plants have been best characterized in Arabidopsis thaliana where dozens of genes have been implicated in converging, complementary, and antagonistic pathways communicating light quality cues perceived by the phytochrome (red/far-red) cryptochrome (blue) and phototropin (blue) photorecptors. Light perception and signaling have been studied in grasses, including rice and sorghum but in much less detail than in Arabidopsis. During the course of the Mocker lab's DOE-funded wrok generating a gene expression atlas in Brachypodium distachyon we observed that Brachypodium plants grown in continuous monochromatic red light or continuous white light enriched in far-red light accumulated significantly more biomass and exhibited significantly greater seed yield than plants grown in monochromatic blue light or white light. This phenomenon was also observed in two other grasses, switchgrass and rice. We will systematically manipulate the expression of genes predicted to function in Brachypodium phytochrome signaling and assess the phenotypic consequences in transgenic Brachypodium plants in terms of morphology, stature, biomass accumulation, and cell wall composition. We will also interrogate direct interactions between candidate phytochrome signaling transcription factors and target promoters using a high-throughput yeast one-hybrid system. Brachypodium distachyon has emerged as a model grass species and is closely related to candidate feedstock crops for bioethanol production. Identification of genes capable of modifying growth characteristics of Brachypodium, when misexpressed, in particular increasing biomass accumulation, by modulating photoreceptor signaling will provide valuable candidates for manipulation in biomass and biofuel feedstock grass crops through targeted breeding or engineering efforts.

09 BIOMASS FUELS↗
DOE OSTI2020

SIGNAL Game Manual

SIGNAL is a first of its kind experimental wargame developed as part of the Project on Nuclear Gaming (PoNG). In this document we describe the rules and game mechanics associated with the online version of SIGNAL created by team members from the University of California, Berkeley, Sandia National Laboratories, and Lawrence Livermore National Laboratory and sponsored by the Carnegie Corporation of New York. The game was developed as part of a larger research project to develop the experimental wargaming methodology and explore its use on a model scenario: the impact of various military capabilities on conflict escalation dynamics. We discuss the results of this research in a forthcoming paper that will include this manual as an appendix. It is our hope that this manual will both contribute to our players' understanding of the game prior to play and that it will allow for replication of the SIGNAL game environment for future research purposes. The manual begins by introducing the terminology used throughout the document. It then outlines the technical requirements required to run SIGNAL. The following section provides a description of the map, resources, infrastructure, tokens, and action cards used in the game environment. The manual then describes the user interface including the chat functions, trade mechanism, currency and population counts necessary for players to plan their actions. It then turns to the sequence of player actions in the game describing the signaling, action, and upkeep phases that comprise each round of play. It then outlines the use of diplomacy including alliances with minor states and trade between players. The manual also describes the process for scoring the game and determining the winner. The manual concludes with tips for players to remember as they embark upon playing the game.

97 MATHEMATICS AND COMPUTING↗
DOE OSTI2023

Examining ICARUS Cosmic Muon Signal Shapes

In the search for new physics, such as sterile neutrinos, we must compare our experimental data to the case where this new physics does not exist, which is provided by simulations. However, our detectors and simulations are not perfect, so we need to be able to differentiate imperfections in our simulations, detector effects, and unknown unknowns from new physics. Thus, we need to quantify ICARUS detector systematic uncertainties; in other words, we need to know how much difference between experiment and simulation we can expect due to only detector systematics, so when we see differences greater than this, we can be confident they are due to new physics. To calculate this uncertainty, we study the signals produced in ICARUS by cosmic muons, since these muons are well understood. Ideally, we want to fit these waveforms as Gaussians and compare the fits from experimental data to fits from simulations to calculate the uncertainties, but first, we need to know how accurately these curve s can be described by Gaussians. We examined the peak and the full width at half maximum (FHWM) of signals from simulations, and we produced plots of the distribution of peaks and FHWMs for these signals. We further studied how the peaks and FHWMs varied depending on where the signal came from in the detector. Ultimately, by comparing the actual distribution of peaks and FHWMs to the distribution predicted by the Gaussian fits, we hope to determine how well these signals can be described as Gaussians.

Patino, Nicolas↗
DOE OSTI2023

Low Energy LArTPC Signal Detection Using Anomaly Detection

Extracting low-energy signals from LArTPC detectors is useful, for example, for detecting supernova events or calibrating the energy scale with argon-39. However, it is difficult to efficiently extract the signals because of noise. We propose using a 1DCNN to select wire traces that have a signal. This efficiently suppresses the background while still being efficient for the signal. This is then followed by a 1D autoencoder to denoise the wire traces. At that point the signal waveform can be cleanly extracted. In order to make this processing efficient, we implement the two networks on an FPGA. In particular we use hls4ml to produce HLS from the Keras models for both the 1DCNN and the autoencoder. We deploy them on an AMD/Xilinx Alveo U55C using the Vitis software platform.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2025

Beam Signal Recovery Using Wiener Deconvolution

This project explores the use of Wiener deconvolution to recover an original signal that has been distorted by a known transfer function and by noise. A simulated Gaussian pulse was used as the test signal, and a transfer function was applied in the frequency domain to model system distortion. Controlled noise was then introduced to approximate real-world signal degradation. A Wiener filter was implemented to reverse the effects of the transfer function while minimizing the influence of noise. The recovered signal was compared to the original pulse to evaluate the effectiveness of the filter. Results demonstrate that Wiener deconvolution offers a stable and effective approach to signal recovery, balancing complete transfer function inversion and noise suppression.

Campbell, Savanna [El Camino Coll.]↗
DOE OSTI2025

Toroid Signal Processing Container with Redis Integration

This report presents the research, design, and implementation of a Toroid Signal Processing Container with Redis Integration, conducted during my internship assignment. The project focused on developing a modular signal processing sys- tem capable of performing baseline correction, droop compensation, and real- time data communication for beam diagnostic signals. Using Redis as a message broker and configuration manager, the system ensures modularity, scalability, and efficient inter-process communication. Signal correction algorithms, such as Asymmetric Least Squares (ALS) baseline smoothing and a numerical droop correction approach, were implemented and tested using Linac beam data. The results confirm that this system improves signal fidelity and supports real-time analysis requirements, offering valuable contributions to the field of beam instrumentation and data acquisition systems.

Bowers, Elliot [Cabrillo Coll.]↗
DOE OSTI2016

VARIATION IN THE PRE-TRANSIT BALMER LINE SIGNAL AROUND THE HOT JUPITER HD 189733B

As followup to our recent detection of a pre-transit signal around HD 189733 b, we obtained full pre-transit phase coverage of a single planetary transit. The pre-transit signal is again detected in the Balmer lines but with variable strength and timing, suggesting that the bow shock geometry reported in our previous work does not describe the signal from the latest transit. We also demonstrate the use of the Ca ii H and K residual core flux as a proxy for the stellar activity level throughout the transit. A moderate trend is found between the pre-transit absorption signal in the 2013 data and the Ca ii H flux. This suggests that some of the 2013 pre-transit hydrogen absorption can be attributed to varying stellar activity levels. A very weak correlation is found between the Ca ii H core flux and the Balmer line absorption in the 2015 transit, hinting at a smaller contribution from stellar activity compared to the 2013 transit. We simulate how varying stellar activity levels can produce changes in the Balmer line transmission spectra. These simulations show that the strength of the 2013 and 2015 pre-transit signals can be reproduced by stellar variability. If the pre-transit signature is attributed to circumplanetary material, its evolution in time can be described by accretion clumps spiraling toward the star, although this interpretation has serious limitations. Further high-cadence monitoring at H α is necessary to distinguish between true absorption by transiting material and short-term variations in the stellar activity level.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2016

The effects of the ionosphere on ground-based detection of the global 21 cm signal from the cosmic dawn and the dark ages

Detection of the global H i 21 cm signal from the Cosmic Dawn and the Epoch of Reionization is the key science driver for several ongoing ground-based and future ground-/space-based experiments. The crucial spectral features in the global 21 cm signal (turning points) occur at low radio frequencies ≲100 MHz. In addition to the human-generated radio frequency interference, Earth’s ionosphere drastically corrupts low-frequency radio observations from the ground. In this paper, we examine the effects of time-varying ionospheric refraction, absorption, and thermal emission at these low radio frequencies and their combined effect on any ground-based global 21 cm experiment. It should be noted that this is the first study of the effect of a dynamic ionosphere on global 21 cm experiments. The fluctuations in the ionosphere are influenced by solar activity with flicker noise characteristics. The same characteristics are reflected in the ionospheric corruption to any radio signal passing through the ionosphere. As a result, any ground-based observations of the faint global 21 cm signal are corrupted by flicker noise (or 1/f noise, where f is the dynamical frequency) which scales as ν{sup −2} (where ν is the frequency of radio observation) in the presence of a bright galactic foreground (∝ν{sup −s}, where s is the radio spectral index). Hence, the calibration of the ionosphere for any such experiment is critical. Any attempt to calibrate the ionospheric effects will be subject to the inaccuracies in the current ionospheric measurements using Global Positioning System (GPS) ionospheric measurements, riometer measurements, ionospheric soundings, etc. Even considering an optimistic improvement in the accuracy of GPS–total electron content measurements, we conclude that Earth’s ionosphere poses a significant challenge in the absolute detection of the global 21 cm signal below 100 MHz.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2018

Equation of State Dependent Dynamics and Multi-messenger Signals from Stellar-mass Black Hole Formation

We investigate axisymmetric black hole (BH) formation and its gravitational wave (GW) and neutrino signals with self-consistent core-collapse supernova simulations of a non-rotating 40 M ⊙ progenitor star using the isotropic diffusion source approximation for the neutrino transport and a modified gravitational potential for general relativistic effects. We consider four different neutron star (NS) equations of state (EoS): LS220, SFHo, BHBΛ$\phi$, and DD2, and study the impact of the EoS on BH formation dynamics and GW emission. We find that the BH formation time is sensitive to the EoS from 460 to >1300 ms and is delayed in multiple dimensions for ~100–250 ms due to the finite entropy effects. Depending on the EoS, our simulations show the possibility that shock revival can occur along with the collapse of the proto-neutron star (PNS) to a BH. The gravitational waveforms contain four major features that are similar to previous studies but show extreme values: (1) a low-frequency signal (~300–500 Hz) from core-bounce and prompt convection, (2) a strong signal from the PNS g-mode oscillation among other features, (3) a high-frequency signal from the PNS inner-core convection, and (4) signals from the standing accretion shock instability and convection. The peak frequency at the onset of BH formation reaches to ~2.3 kHz. Thus, the characteristic amplitude of a 10 kpc object at peak frequency is detectable but close to the noise threshold of the Advanced LIGO and KAGRA, suggesting that the next-generation GW detector will need to improve the sensitivity at the kHz domain to better observe stellar-mass BH formation from core-collapse supernovae or failed supernovae.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1972

On the detection of differentially encoded polyphase signals.

Discussion of the transmission and detection of differentially encoded polyphase signals and of the ambiguity resolution problem which results from suppression of the transmitted carrier. In particular, an analysis is made of the performance of differentially encoded coherent multiple phase-shift keyed (MPSK) systems which reconstruct coherent reference signals by means of generalized Costas or nth-power loops. The performance of such systems is then compared with that of ideal reception of MPSK signals and differentially coherent detection of differentially encoded MPSK signals. Emphasis is placed upon the special cases of quadriphase and octaphase signaling.

Lindsey, W. C.↗
NASA NTRS1972

L-orthogonal signal transmission and detection.

A study of L-orthogonal signal performance represents an approach wherein the performance of polyphase and orthogonal signal sets stand out as the extreme special cases. The L-orthogonal signal model and its associated bandwidth properties are briefly discussed. System performance is investigated by presenting tight upper and lower bounds on the error probability performance of the maximum-likelihood receiver of L-orthogonal signals suggested by Reed and Scholtz (1966). A study of the error probability performance of the suboptimum receiver in the presence of noisy reference signals is also conducted.

Lindsey, W. C.↗
NASA NTRS1972

Data Transmission Signal Design and Analysis

The error performances of several digital signaling methods are determined as a function of a specified signal-to-noise ratio. Results are obtained for Gaussian noise and impulse noise. Performance of a receiver for differentially encoded biphase signaling is obtained by extending the results of differential phase shift keying. The analysis presented obtains a closed-form answer through the use of some simplifying assumptions. The results give an insight into the analysis problem, however, the actual error performance may show a degradation because of the assumptions made in the analysis. Bipolar signaling decision-threshold selection is investigated. The optimum threshold depends on the signal-to-noise ratio and requires the use of an adaptive receiver.

Moore, J. D.↗
NASA NTRS1968

Low level signal limiter

A limiting circuit is described which prevents a signal being supplied to a signal amplifier from exceeding a predetermined value. The circuit is designed to permit a signal voltage to be fed directly to a signal amplifier without passing through the operational amplifier and without being altered undesirably. When the signal level increases to the predetermined value, the summing point shifts from the input of the operational amplifier to the output of the limiting circuit.

Hansen, I. G.↗
NASA NTRS1974

High isolation RF signal selection switches

A selection switch with high isolation between RF signal input terminals is achieved with a gated Schmitt trigger circuit feeding into a control NAND gate in each signal switching channel. The control NAND gates of the separate signal channels are coupled to an output terminal by a single NAND gate. The schmitt trigger circuits and all gates are implemented with Schottky transistor-transistor logic circuits having input clamping diodes. Each Schmitt trigger circuit includes two cascaded NAND gates and a feedback isolation Schottky diode between one input terminal connected to receive an RF input and another input terminal connected to receive a feedback signal from the second of the two cascaded NAND gates. Both NAND gates of the Schmitt trigger circuits are enabled by the same switch control signal which enables the control gates.

Hanna, M. F.↗