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At least 721 records · Page 40

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NASA NTRS1986

The calibration of photographic and spectroscopic films. 1: Film batch variations of reciprocity failure in IIaO film. 2: Thermal and aging effects in relationship to reciprocity failure. 3: Shifting of reciprocity failure points as a function of thermal and aging effects

Reciprocity failure was examined for IIaO spectroscopic film. Three separate experiments were performed in order to study film batch variations, thermal and aging effects in relationship to reciprocity failure, and shifting of reciprocity failure points as a function of thermal and aging effects. The failure was examined over ranges of time between 5 and 60 seconds. The variation to illuminance was obtained by using thirty neutral density filters. A standard sensitometer device imprinted the wedge pattern on the film as exposure time was subjected to variation. The results indicate that film batch differences, temperature, and aging play an important role in reciprocity failure of IIaO spectroscopic film. A shifting of the failure points was also observed in various batches of film.

Peters, K. A.↗

NASA NTRS1987

The calibration of photographic and spectroscopic films. Part 1: Film batch variations of reciprocity failure in IIaO film. Part 2: Thermal and aging effects in relationship to reciprocity failure. P art 3: Shifting of reciprocity failure points as a function of thermal and aging effects

Reciprocity failure was examined for IIaO spectroscopic film. Three separate experiments were performed in order to study film batch variations, thermal and aging effects in relationship to reciprocity failure, and shifting of reciprocity failure points as a function of thermal and aging effects. The failure was examined over ranges of time between 5 and 60 seconds. The variation to illuminance was obtained by using thirty neutral density filters. A standard sensitometer device imprinted the wedge pattern on the film as exposure time was subjected to variation. Results indicate that film batch differences, temperature, and aging play an important role in reciprocity failure of IIaO spectroscopic film. A shifting of the failure points was also observed in various batches of film.

Peters, Kevin A.↗

NASA NTRS1987

Theoretical study of the high-latitude ionosphere's response to multicell convection patterns

A time-dependent three-dimensional model of the high-latitude ionosphere is used to study the characteristic ionospheric signatures associated with two-, three-, and four-cell plasma convection patterns. It is found that, for two-cell convection, the antisunward flow of plasma from the dayside into the polar cap acts to maintain the densities in this region in winter. For four-cell convection, the two additional convection cells in the polar cap are in darkness most of the time, and the resulting O(+) decay acts to produce twin polar holes that are separated by a sun-aligned ridge of enhanced ionization due to theta-auroral precipitation. For three-cell convection, only one polar hole forms in the total electron density, and an additional O(+) depletion region develops near noon. In this region there are strong electric fields, high ion temperatures, and an enhanced rate of O(+) - NO(+) conversion.

Sojka, J. J.↗

NASA NTRS1987

Proton gradients in intact cyanobacteria

The internal pH values of two unicellular cyanobacterial strains were determined with electron spin resonance probes, over an external pH range of 6 to 9, in the light and in the dark. The slow growing, thylakoid-lacking Gloeobacter violaceus was found to have a low capacity for maintaining a constant internal pH. The distribution pattern of weak acid and amine nitroxide spin probes across the cell membranes of this organism, in the light and in the dark, was consistent with the assumption that it contains a single intracellular compartment. At an external pH of 7.0, intracellular pH was 6.8 in the dark and 7.2 in the light. The cells of Agmenellum quadruplicatum, a marine species, were found to contain two separate compartments; in the dark, the pH of the cytoplasmic and the intrathylakoid spaces were calculated to be 7.2 and 5.5, respectively. Upon illumination, the former increased and the latter decreased by about 0.5 pH units.

Non-NASA Center↗

NASA NTRS2012

Arctic Tundra Vegetation Functional Types Based on Photosynthetic Physiology and Optical Properties

Climate change in tundra regions may alter vegetation species composition and ecosystem carbon balance. Remote sensing provides critical tools for monitoring these changes as optical signals provide a way to scale from plot measurements to regional patterns. Gas exchange measurements of pure patches of key vegetation functional types (lichens, mosses, and vascular plants) in sedge tundra at Barrow AK, show three significantly different values of light use efficiency (LUE) with values of 0.013+/-0.001, 0.0018+/-0.0002, and 0.0012 0.0001 mol C/mol absorbed quanta for vascular plants, mosses and lichens, respectively. Further, discriminant analysis of patch reflectance identifies five spectral bands that can separate each vegetation functional type as well as nongreen material (bare soil, standing water, and dead leaves). These results were tested along a 100 m transect where midsummer spectral reflectance and vegetation coverage were measured at one meter intervals. Area-averaged canopy LUE estimated from coverage fractions of the three functional types varied widely, even over short distances. Patch-level statistical discriminant functions applied to in situ hyperspectral reflectance successfully unmixed cover fractions of the vegetation functional types. These functions, developed from the tram data, were applied to 30 m spatial resolution Earth Observing-1 Hyperion imaging spectrometer data to examine regional variability in distribution of the vegetation functional types and from those distributions, the variability of LUE. Across the landscape, there was a fivefold variation in tundra LUE that was correlated to a spectral vegetation index developed to detect vegetation chlorophyll content.

Huemmrich, Karl F.↗

NASA NTRS1974

Lunar orbit determination in the presence of unmodeled accelerations

A technique for estimating the state of an artificial satellite in the presence of unmodeled accelerations is presented. The unmodeled acceleration is approximated by a first-order Gauss-Markov sequence which can be separated into a timewise-correlated component and a purely random component. Using this approximation, a sequential procedure for estimating the position, velocity, and the unmodeled acceleration is developed. The method is evaluated by reducing range-rate observations obtained by tracking the Apollo 10 and 11 spacecraft during the lunar-orbit phase of the mission. Numerical results are presented which show that the observation residual pattern lies within the observation noise standard deviation. The values of the estimated components of the unmodeled acceleration are repeatable from orbit to orbit within a given mission and from mission to mission when the same ground track is covered. Finally, the variation in the radial component of the unmodeled acceleration shows a high correlation with the reported location of the lunar surface mascons.

Ingram, D. S.↗

NASA NTRS1979

An auroral effect on the fair weather electric field

Evidence is presented for coupling between the upper and lower atmosphere by means of the shorting out of the vertical mesospheric electric field by auroral radiation, which causes a transfer of mesospheric potential to the lower atmosphere. Measurements were made by an electric field antenna which was part of a parachute-borne payload, launched by rocket from near Fairbanks, Alaska. Data obtained from quiet time observations indicate the normal low altitude electric field pattern, with a greater field at high altitudes, while observations at disturbed times show a small field at high altitudes and a greater field at low altitudes. Means for observing this effect at lower latitudes are also suggested. While the data obtained support the proposed mechanism, it is noted that other mechanisms, such as direct modulation by large amounts of solar cosmic rays and aurorally produced charge separation, may also be important.

Hale, L. C.↗

NASA NTRS1985

Magnetic shear. II - Hale region 17244

A B-gamma(delta) sunspot group with growing delta-spots of trailing polarity shows evidence in H-alpha filament structure of a transition from a state of weak magnetic shear to a state of strong shear. The shear develops in the chromosphere and transition region to the corona overlying the photospheric magnetic neutral line separating the delta-spots from the leading polarity at a time when the delta-spots are undergoing rapid growth. Several major flares occur along the sheared portion of the neutral line following the shear development. Other segments of the neutral line far removed from the delta-spots show similar evidence of shear in the H-alpha filament structure and in C IV velocity patterns as well. These 'quiescent' regions of shear are relatively steady or decaying with time and show very little related activity.

Athay, R. G.↗

NASA NTRS1985

Hormone Purification by Isoelectric Focusing

Various ground-based research approaches are being applied to a more definitive evaluation of the natures and degrees of electroosmosis effects on the separation capabilities of the Isoelectric Focusing (IEF) process. A primary instrumental system for this work involves rotationally stabilized, horizontal electrophoretic columns specially adapted for the IEF process. Representative adaptations include segmentation, baffles/screens, and surface coatings. Comparative performance and development testing are pursued against the type of column or cell established as an engineering model. Previously developed computer simulation capabilities are used to predict low-gravity behavior patterns and performance for IEF apparatus geometries of direct project interest. Three existing mathematical models plus potential new routines for particular aspects of simulating instrument fluid patterns with varied wall electroosmosis influences are being exercised.

Bier, M.↗

NASA NTRS2004

Antigenic Protein In Microgravity-Grown Human Mixed Mullerian Tumor (LN1) Cells Preserved In RNA Stabilizing Agent

Cells treated with RNAlater(TradeMark) have previously been shown to contain antigenic proteins that can be visualized using Western blot analysis. These proteins seem to be stable for several months when stored in RNA stabilizer at 4 C. Antigenic protein can be recovered from cells that have been processed using an Ambion RNAqueous(Registered TradeMark) kit to remove RNA. In this set of experiments, human mixed Mullerian tumor (LNI) cells grown on the International Space Station during Expedition 3 were examined for antigenic stability after removal of RNA. The cells were stored for three months in RNAlater(TradeMark) and RNA was extracted. The RNA filtrate containing the protein was precipitated, washed, and suspended in buffer containing sodium dodecyl sulfate (SDS). Samples containing equal concentrations of protein were loaded onto SDS-polyacrylamide gels. Proteins were separated by electrophoresis and transferred by Western blot to polyvinylidene fluoride (PVDF) membrane. The Western blots were stained with an enhanced chemiluminescent ECL(Registered Trademark) Plus detection kit (Amersham) and scanned using a Storm 840 gel image analyzer (Amersham, Molecular Dynamics). ImageQuant(Registered TradeMark) software was used to quantify the densities of the protein bands. The ground control and flight LN1 cell samples showed a similar staining pattern over time with antibodies to vimentin, glyceraldehyde-3-phosphate dehydrogenase, and epithelial membrane antigens.

Hammond, Dianne K.↗

NASA NTRS1986

Intercomparison between Nimbus-7 cirrus cloud data and ERB measurements

Nimbus-7 and Earth Radiation Budget (ERB) data were compared with the quantity of high, thin cirrus clouds over a 6 yr period. Three separate data analysis techniques are described which were used to derive cloud cover and total upwelling radiance values from the satellite data. Zonal average cirrus clouds amounts are compared with surface observations and SAGE satellite data, and comparisons are made between the cloud amount estimates made with ERB data and data from other Nimbus-7 instruments. All Nimbus-7 instrumental data indicated cloud amounts and frequency of occurrence patterns which were commensurate with surface observations, except in high latitude zones.

Yeh, H. Y. M.↗

NASA NTRS1988

Optical Recognition And Tracking Of Objects

Separate objects moving independently tracked simultaneously. System uses coherent optical techniques to obtain correlation between each object and reference image. Moving objects monitored by charge-coupled-device television camera, output fed to liquid-crystal television (LCTV) display. Acting as spatial light modulator, LCTV impresses images of moving objects on collimated laser beam. Beam spatially low-pass filtered to remove high-spatial-frequency television grid pattern.

Chao, Tien-Hsin↗

NASA NTRS2004

Antigenic Protein In Microgravity-Grown Human Mixed Mullerian Tumor (LN1) Cells Preserved In RNA Stabilizing Agent

Cells treated with RNAlater(TradeMark) have previously been shown to contain antigenic proteins that can be visualized using Western blot analysis. These proteins seem to be stable for several months when stored in RNA stabilizer at 4 C. Antigenic protein can be recovered from cells that have been processed using an Ambion RNAqueous(Registered TradeMark) kit to remove RNA. In this set of experiments, human mixed Mullerian tumor (LN1) cells grown on the International Space Station during Expedition 3 were examined for antigenic stability after removal of RNA. The cells were stored for three months in RNAlater(TradeMark) and RNA was extracted. The RNA filtrate Containing the protein was precipitated, washed, and suspended in buffer containing sodium dodecyl sulfate (SDS). Samples containing equal concentrations of protein were loaded onto SDS-polyacrylamide gels. Proteins were separated by electrophoresis and transferred by Western blot to polyvinylidene fluoride (PVDF) membrane. The Western blots were stained with an enhanced chemiluminescent ECL(Registered TradeMark)Plus detection kit (Amersham) and scanned using a Storm 840 gel image analyzer (Amersham, Molecular Dynamics). ImageQuant(Registered TradeMark)a software was used to quantify the densities of the protein bands. The ground control and flight LN1 cell samples showed a similar staining pattern over time with antibodies to vimentin, glyceraldehyde-3-phosphate dehydrogenase, and epithelial membrane antigens.

Hammond, Dianne K.↗

NASA NTRS2001

Apparatus and method using a holographic optical element for converting a spectral distribution to image points

A holographic optical element transforms a spectral distribution of light to image points. The element comprises areas, each of which acts as a separate lens to image the light incident in its area to an image point. Each area contains the recorded hologram of a point source object. The image points can be made to lie in a line in the same focal plane so as to align with a linear array detector. A version of the element has been developed that has concentric equal areas to match the circular fringe pattern of a Fabry-Perot interferometer. The element has high transmission efficiency, and when coupled with high quantum efficiency solid state detectors, provides an efficient photon-collecting detection system. The element may be used as part of the detection system in a direct detection Doppler lidar system or multiple field of view lidar system.

McGill, Matthew J.↗

NASA NTRS2020

Integrated Modeling and Development of Component-Based Embedded Software in Scala

Programming of embedded systems is challenging due to the low-level design patterns normally applied in the implementation of such systems. Furthermore, programming languages normally considered suitable for this level of programming, such as C and C++, are themselves low-level compared to more modern programming languages. We report on an effort exploring modeling and programming of embedded systems in modern high-level programming languages combining object-oriented and functional programming. We present an integration of four separate internal DSLs (libraries), considered useful for embedded program- ming, in the Scala programming language, for programming and testing component-based systems. These include a DSL for defining components and their connections, and a DSL for programming the individual components as hierarchical state machines. Two additional DSLs support testing, and include a DSL for writing temporal logic flavored test oracles for monitoring program executions, and a DSL for rule-based test input generation. The paper discusses the gap between Scala as used here and the needs for embedded systems programming.

Bocchino, Robert↗

NASA NTRS1999

The Vertical Structure of Major Meteorological Features on Jupiter: The Great Red Spot and White Ovals BC and DE

Multi-spectral imagery of Jupiter's Great Red Spot (GRS) and two White ovals acquired by the Galileo/NIMS are used to constrain the spatial variability of the vertical aerosol structure and the distribution of ammonia in and around these most-prominent anti-cyclonic features. All three features exhibit a high-altitude core spanning about 3/4 of their visual size when viewed with moderate absorption wavelengths, indicating a bulk elliptical, "wedding cake" shape in their overall three-dimensional cloud structure. A distinctive spiral pattern within the GRS core is seen in moderate methane and hydrogen absorption bandpasses. This pattern - which has been modelled to show a 2 km variation in cloudtop pressure within the GRS - is inconsistent with a different spiral-shaped pattern observed in ammonia-sensitive wavelengths, thus indicating spatial variability not only in the column abundance of ammonia within the GRS, but in its mixing ratio as well. White Ovals BC and DE were observed in February 1997, just a year before their unusual merger into a single feature. At the time of these observations, the centers of the two anti-cyclones were about 16 degrees apart, separated by a complex cyclonic feature which exhibited unusual spatial variability in its appearance in images acquired at ammonia-sensitive wavelengths. In particular, the northern half of this feature has the largest ammonia column abundance seen within the environs around the white ovals, indicating unusual variability in either cloud structure/altitude and/or ammonia humidity within the cyclone.

Baines, Kevin H.↗

NASA NTRS2006

Initial Results of the Geosynchronous Synthetic Thinned Array Radiometer (GeoSTAR)

An error budget is presented to meet 1 Kelvin radiometric accuracy in a geostationary atmospheric sounder with 50 km spatial resolution on the earth. The gain and phase errors are weighted by the magnitude of visibility versus antenna separation, and requirements range between approx.0.5% and 0.3 degrees of amplitude and phase, respectively, for the closest spacings at the center of the array, and about 5% and 3 degrees for the majority of the array. The latter requirement is met by our design without any special testing or stabilizations by reference signals. The former is met using an internal noise diode reference and by measuring the detailed antenna patterns on the antenna range. Biases and other additive errors in the raw visibility samples must be below about 2 mK on average, and this requirement is met by a phase shifting scheme applied to the local oscillator distribution. An outline of the data processing is presented, along with the first images from this system.

Synthetic Thinned Aperture Radiometer (STAR)↗

NASA NTRS1952

The Cylinder and Semicylinder in Subsonic Flow

In studying the diffraction of shock waves around various two-dimensional obstacles we have observed that flow separation and the formation of vortices contributes in an important way to transient loading of the obstacle. The cases of a cylinder and semicylinder are especially interesting because the breakaway point is not clearly defined as it is for objects having sharp corners. Accordingly a number of experiments have been made in the shock tube to observe the influence of Reynolds number and Mach number on the transient flow patterns about a cylinder and about a semicylinder mounted on a smooth plane. Some differences might be anticipated since the plane would impose a symmetry on the flow and produce a viscous boundary layer for which there is no counterpart with the cylinder. In the course of these experiments it was noted that a condition of steady subsonic flow about both the cylinder and semicylinder was approached. Thus a comparison with von Karrnan's theoretical calculation of the drag on a cylinder, from certain characteristics of its wake or "vortex street", was undertaken.

Bingham, Harry J.↗