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At least 721 records · Page 40

Wind Fields in Category 1–3 Tropical Cyclones Are Not Fully Represented in Wind Turbine Design Standards

Abstract Offshore wind energy deployment in the U.S. is expected to increase in the years to come, with proposed wind farm sites located in regions with high risk for tropical cyclones. Yet, the wind turbine design criteria outlined by the International Electrotechnical Commission for extreme events may not account for wind field characteristics unique to tropical cyclones. To evaluate if current design standards capture the extreme conditions of these storms, we perform idealized large‐eddy simulations of three tropical cyclones (one Category 1, one Category 2, and one Category 3 storms) using the Weather Research and Forecasting model. Wind conditions near the eyewall of Cat‐1, Cat‐2, and Cat‐3 storms can exceed current design standards for offshore wind turbines. Winds at 90 m can be faster than outlined in the design criteria for Class I and Class T turbines for 50‐year recurrence periods. Wind speed shear across the turbine rotor layer is also larger than assumed in design specifications. Moreover, vertical variations in wind direction across the turbine rotor layer are large for tropical cyclones of all intensity levels, suggesting design standards should include veer, which can amplify loads in wind turbines. The existence of hurricane wind field characteristics that are not represented in design standards does not imply that damage or failure will certainly occur. Engineering safety factors incorporated in the design of the turbine's blades and structural components may prevent damage from occurring.

17 WIND ENERGY↗

Dynamics of pectic homogalacturonan in cellular morphogenesis and adhesion, wall integrity sensing and plant development

Homogalacturonan (HG) is the most abundant pectin subtype in plant cell walls. Although it is a linear homopolymer, its modification states allow for complex molecular encoding. HG metabolism affects its structure, chemical properties, mobility and binding capacity, allowing it to interact dynamically with other polymers during wall assembly and remodelling and to facilitate anisotropic cell growth, cell adhesion and separation, and organ morphogenesis. HGs have also recently been found to function as signalling molecules that transmit information about wall integrity to the cell. Here we highlight recent advances in our understanding of the dual functions of HG as a dynamic structural component of the cell wall and an initiator of intrinsic and environmental signalling. Here, we also predict how HG might interconnect the cell wall, plasma membrane and intracellular components with transcriptional networks to regulate plant growth and development.

59 BASIC BIOLOGICAL SCIENCES↗

Atomic resolution tracking of nerve-agent simulant decomposition and host metal–organic framework response in real space

Gas capture and sequestration are valuable properties of metal–organic frameworks (MOFs) driving tremendous interest in their use as filtration materials for chemical warfare agents. Recently, the Zr-based MOF UiO-67 was shown to effectively adsorb and decompose the nerve-agent simulant, dimethyl methylphosphonate (DMMP). Understanding mechanisms of MOF-agent interaction is challenging due to the need to distinguish between the roles of the MOF framework and its particular sites for the activation and sequestration process. Here, we demonstrate the quantitative tracking of both framework and binding component structures using in situ X-ray total scattering measurements of UiO-67 under DMMP exposure, pair distribution function analysis, and theoretical calculations. The sorption and desorption of DMMP within the pores, association with linker-deficient Zr6 cores, and decomposition to irreversibly bound methyl methylphosphonate were directly observed and analyzed with atomic resolution.

36 MATERIALS SCIENCE↗

Heterogeneous techniques for rescaling energy deposits in the CMS Phase-2 endcap calorimeter

We present the porting to heterogeneous architectures of the algorithm used for applying linear transformations of raw energy deposits in the CMS High Granularity Calorimeter (HGCAL). This is the first heterogeneous algorithm to be fully integrated with HGCAL’s reconstruction chain. After introducing the latter and giving a brief description of the structural components of HGCAL relevant for this work, the role of the linear transformations in the calibration is reviewed. The many ways in which parallelization is achieved are described, and the successful validation of the heterogeneous algorithm is covered. Detailed performance measurements are presented, including throughput and execution time for both CPU and GPU algorithms, therefore establishing the corresponding speedup. We finally discuss the interplay between this work and the porting of other algorithms in the existing reconstruction chain, as well as integrating algorithms previously ported but not yet integrated.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Excited nucleon spectrum and structure studies with CLAS and CLAS12

The study of the spectrum and structure of excited nucleon states employing the electroproduction of exclusive reactions is an important avenue for exploring the nature of the non-perturbative strong interaction. The CLAS detector in Hall B has provided the dominant part of the available world data on most relevant meson electroproduction channels off the nucleon in the resonance region for Q2 up to 5 GeV2. Analyses of CLAS data for the exclusive channels πN, ηN, and π+π-p on a proton target have provided the only results available on the Q2 evolution of the electro-excitation amplitudes for the transitions from the initial photon-proton to the final N* states in the mass range up to W =1.8 GeV. These electrocouplings allow for exploration of the internal structure of the produced excited nucleon states. This work has made it clear that consistent results from independent analyses of several exclusive channels with different resonance hadronic decay parameters and non-resonant backgrounds but the same N* electro-excitation amplitudes, is essential to have confidence in the extracted results. Starting in early 2018, a program to study the spectrum and structure of N* states in various exclusive electroproduction channels using the new CLAS12 spectrometer commenced. These studies will probe the structure of N* states in the mass range up to W =3 GeV and for Q2 as low as 0.05 GeV2 and as high as 10-12 GeV2, thus providing a means to access N* structure information spanning a broad range of distance scales. Quasi-real photoproduction studies are also planned to search for additional N* states, the so-called hybrid baryons, for which the glue serves as an active structural component. In this talk the N* programs from both CLAS and CLAS12 will be reviewed.

Carman, D. S.↗

A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1

Lignocellulose, the structural component of plant cells, is a major agricultural byproduct and the most abundant terrestrial source of biopolymers on Earth. The complex and insoluble nature of lignocellulose limits its conversion into value-added commodities, and currently, efficient transformation requires expensive pretreatments and high loadings of enzymes. In this work, we report on a fungus from the Parascedosporium genus, isolated from a wheat-straw composting community, that secretes a large and diverse array of carbohydrate-active enzymes (CAZymes) when grown on lignocellulosic substrates. We describe an oxidase activity that cleaves the major β-ether units in lignin, thereby releasing the flavonoid tricin from monocot lignin and enhancing the digestion of lignocellulose by polysaccharidase mixtures. We show that the enzyme, which holds potential for the biorefining industry, is widely distributed among lignocellulose-degrading fungi from the Sordariomycetes phylum.

59 BASIC BIOLOGICAL SCIENCES↗

Highly stable preferential carbon monoxide oxidation by dinuclear heterogeneous catalysts

Atomically dispersed catalysts have been shown highly active for preferential oxidation of carbon monoxide in the presence of excess hydrogen (PROX). However, their stability has been less than ideal. We show here that the introduction of a structural component to minimize diffusion of the active metal center can greatly improve the stability without compromising the activity. Using an Ir dinuclear heterogeneous catalyst (DHC) as a study platform, we identify two types of oxygen species, interfacial and bridge, that work in concert to enable both activity and stability. In conclusion, the work sheds important light on the synergistic effect between the active metal center and the supporting substrate and may find broad applications for the use of atomically dispersed catalysts.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Impact of Radial Reflector Fidelity on Neutronics and Vessel Fluence Simulations

The Consortium for Advanced Simulation of Light Water Reactors is developing the Virtual Environment for Reactor Applications (VERA), and the MPACT code, which is the primary deterministic neutron transport solver in VERA, provides sub-pin level flux and power distributions as part of full-scale cycle depletion and analysis. In such calculations, an important aspect is the radial reflector treatment. To improve the fidelity of the radial reflector treatment, MPACT was extended to approximate the modeling of the reactor’s structural components such as the core shroud, barrel, neutron pads, and vessel. This work explores several modeling configurations with varying levels of fidelity and computational burden and assesses the importance of modeling fidelity on the eigenvalue and pin power distribution. Two two-dimensional (2-D) problems were analyzed to assess the impact on eigenvalue and pin power distributions with low-fidelity, coarse square cell reflector representations: (1) a Watts Bar Nuclear Plant Unit 1 (WBN1) quarter-core slice with depletion and (2) an AP1000 quarter-core slice. In this work, the analyses showed that the effect on eigenvalue is fairly small, but the effect on pin power is more pronounced, especially locally in the assemblies closest to the periphery, where the maximum pin power difference is nearly 3.5% in the AP1000 case. Two additional 2-D problems were used to assess the comparison between the low-fidelity coarse square cell treatment and a high-fidelity geometric representation that uses subpin material specification: (1) the same WBN1 quarter-core slice and (2) a representative model of the NuScale small modular reactor (SMR), which features a solid reflector design with moderator holes. These results demonstrate that even a coarse, low-fidelity representation adequately captures the necessary simulation characteristics. Last, these capabilities were applied to the 2-D WBN1 quarter-core depletion to assess the impact on vessel fluence using VeraShift. From adjoint calculations, pins along the periphery were observed to be of highest importance for fluence calculation, so the impact of the reflector representation in MPACT could theoretically substantially affect the predicted result. However, it was observed that the change in pin powers along the periphery minimally impacts the maximum vessel fluence with a difference within the statistical uncertainty but provides terrific insight on the sensitivity of the peripheral pins.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Simulation of the Fast Reactor Fuel Assembly Duct-Bowing Reactivity Effect Using Monte Carlo Neutron Transport and Finite Element Analysis

This paper discusses a new method of simulating the fuel assembly duct-bowing reactivity coefficient for EBR-II run 138B. Quantification of the fuel assembly duct-bowing reactivity effect in liquid metal–cooled fast reactors has been a persistent problem since they were first designed and operated. Simulation of the duct-bowing reactivity effect is difficult because the level of detail required to simulate the effect has exceeded most modeling capabilities. The new method outlined in this paper utilizes the finite element analysis code ANSYS to analyze the thermal and structural components. Here, the displacement of the fuel assembly duct due to thermal expansion and mechanical interaction was calculated by ANSYS using recorded EBR-II run 138B temperature and power boundary value data. The displacement values were incorporated into to a Monte Carlo model of EBR-II run 138B and keff was calculated. Multiple Monte Carlo calculations were performed with duct displacement values corresponding to different reactor temperatures. Using the calculated keff values associated with the different duct displacement results allowed calculation of the duct-bowing reactivity coefficient. The duct-bowing reactivity coefficient was calculated to be –14.5 × 10 –4 $/°C/ ± 4.4%.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Assessment of RELAP5-3D Code with Molten Salt Heat Transfer Experiments

The accurate thermal-hydraulic assessment of thermal storage systems is crucial for addressing the integrity and performance of the storage system design. This is particularly important for thermal storage systems using molten salts as a heat transport and storage medium, as the chemical corrosion and erosion by the high-temperature molten salts can cause serious damage to the structural components of the storage system. To understand these effects for TerraPower’s Natrium® Demonstration Reactor design, the system thermal-hydraulic analysis code, RELAP5-3D, is utilized to analyze the thermal storage system. This study evaluates two heat transfer correlations implemented in the RELAP5-3D code—the Dittus-Boelter and Gnielinski correlations—using selected benchmark cases from two molten salt heat transfer experiments conducted at Xi’an Jiao Tong University and the German Aerospace Center. The Nusselt numbers calculated using the Gnielinski correlation agree with the experimental data within ±5% of the relative deviations at 300°C and 400°C. With the increasing Reynolds numbers, the Dittus-Boelter correlation underestimates the Nusselt numbers by up to 22% compared to the Gnielinski correlation. Comparison of the RELAP5-3D calculation results with experimental data at a high temperature of 550°C revealed that the thermo-chemical characteristics of solar salt at high temperatures surpassing the decomposition temperatures of nitrate salts reduce the accuracy of heat transfer model of the RELAP5-3D code. Specifically, it has been observed that the temperatures of the wall surface and liquid near the wall can surpass the decomposition temperatures, even though the bulk temperature of molten salts remains significantly lower than these decomposition temperatures. In summary, this study recommends the use of the Gnielinski correlation for the heat transfer analysis of molten salt, rather than the Dittus-Boelter correlation.

21 - SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLAN↗

AR reactor neutronics multi-code validation*

The affordable, robust, compact (ARC) reactor is a tokamak fusion reactor concept currently under development by Commonwealth Fusion Systems and Massachusetts Institute of Technology. There are three important neutronics considerations for the operation of the ARC reactor: (1) breeding of enough tritium in the blanket to sustain the D–T reaction in the plasma; (2) ensuring low fluence on the superconducting toroidal field coils; and (3) assessing neutron volumetric heating in structural components. Here, this work aims to perform a validation of the neutronics analysis approach by code-to-code comparison. State-of-the-art software stacks are employed for the neutronics analysis of the ARC reactor, and a computer-aided design (CAD) model is used directly for Monte Carlo (MC) neutron transport calculations. Three software stacks, Attila-MCNP, OpenMC-DAGMC, and Shift-DAGMC, are used to perform neutronic analyses of a 90° sector CAD model of the ARC reactor. In conclusion, results show that the flux tallies calculated by the three software stacks are very close. Also, the volumetric heating and tritium breeding values have less than 0.6% relative difference between codes.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Development and construction status of the Mu2e electromagnetic calorimeter mechanical structures

Abstract The Mu2e experiment at Fermilab will search for the CLFV neutrino-less coherent conversion of a muon into an electron in the field of an aluminum nucleus. The observation of this process would be the evidence of physics beyond the Standard Model. Mu2e comprises a straw-tracker, an electromagnetic calorimeter and an external veto for cosmic rays. The calorimeter provides electron identification, a fast trigger and aids track reconstruction. It is a state-of-the-art crystal calorimeter and employs 1340 pure CsI crystals readout by UV-extended SiPM and fast electronics. The design consists of two identical annular disks positioned at the relative distance of 70 cm downstream the target.The hostile Mu2e conditions (total ionizing dose of 12 krad and a neutron fluence of 5 × 10 10 n/cm 2 @ 1 MeVeq (Si)/y, 1 T magnetic field and vacuum level of 10 −4 Torr) posed tight constraints on the mechanical structures and materials choice. The support structure of the two crystal matrices employs two aluminum hollow rings and parts made of open-cell vacuum-compatible carbon fiber. SiPMs and front-end electronics for each crystal are assembled in one mechanical unit inserted in a machined copper holder. The units are supported by a plate made of vacuum-compatible material. The plate integrates the cooling system made of a network of copper lines flowing a low temperature fluid and placed in thermal contact with the copper holders. The DAQ is hosted in aluminum crates positioned on the lateral surface of the disks. The crates also integrate the DAQ electronics cooling system. We review the constraints on the calorimeter structures design, the development of all the structural components, including the simulations that have determined the materials and technological choices and the specifications of the cooling station, components production and quality assurance tests, the procedures for detector assembly, transportation and installation in the experimental area.

Instruments & Instrumentation↗

Chapter 8: Searching for Life Beyond Earth

The search for life beyond Earth necessitates a rigorous and comprehensive examination of biosignatures, the types of observable imprints that life produces. These imprints and our ability to detect them with advanced instrumentation hold the key to our understanding of the presence and abundance of life in the universe. Biosignatures are the chemical or physical features associated with past or present life and may include the distribution of elements and molecules, alone or in combination, as well as changes in structural components or physical processes that would be distinct from an abiotic background. The scientific and technical strategies used to search for life on other planets include those that can be conducted in situ to planetary bodies and those that could be observed remotely. Here this chapter discusses numerous strategies that can be employed to look for biosignatures directly on other planetary bodies using robotic exploration including those that have been deployed to other planetary bodies, are currently being developed for flight, or will become a critical technology on future missions. Search strategies for remote observations using current and planned ground-based and space-based telescopes are also described. Evidence from spectral absorption, emission, or transmission features can be used to search for remote biosignatures and technosignatures. Improving our understanding of biosignatures, their production, transformation, and preservation on Earth can enhance our search efforts to detect life on other planets.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Hubble Space Telescope Captures UGC 12591: bulge/disc properties, star formation and ‘missing baryons’ census in a very massive and fast-spinning hybrid galaxy

We present Hubble Space Telescope (HST) observations of the nearby, massive, highly rotating hybrid galaxy UGC 12591, along with observations in the UV to FIRbands. HST data in V, I, and H bands is used to disentangle the structural components. Surface photometry shows a dominance of the bulge over the disc with an H-band B/D ratio of $69{{\,\rm per\,cent}}$. The spectral energy distribution (SED) fitting reveals an extremely low global star formation rate (SFR) of $\rm \sim 0.1\!-\!0.2\, M_\odot \, yr^{-1}$, exceptionally low for the galaxy’s huge stellar mass of $\rm 1.6\times 10^{11}M_\odot$, implying a strong quenching of its SFR with a star formation efficiency of 3–5 per cent. For at least the past $\rm 10^{8}$ yr, the galaxy has remained in a quiescent state as a sterile, ‘red and dead’ galaxy. UGC 12591 hosts a supermassive black hole (SMBH) of $\rm 6.18\times 10^{8} \, M_\odot$, which is possibly quiescent at present, i.e. we neither see large ($\rm \gt 1 kpc$) radio jets nor the SMBH contributing significantly to the mid-IR SED, ruling out strong radiative feedback of AGN. We obtained a detailed census of all observable baryons with a total mass of $\rm 6.46\times 10^{11} \, M_\odot$ within the virial radius, amounting to a baryonic deficiency of ${\sim }85{{\,\rm per\,cent}}$ relative to the cosmological mean. Only a small fraction of these baryons reside in a warm/hot circumgalactic X-ray halo, while the majority are still unobservable. We discussed various astrophysical scenarios to explain its unusual properties. Our work is a major step forward in understanding the assembly history of such extremely massive, isolated galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗

A compact stem-loop DNA aptamer targets a uracil-binding pocket in the SARS-CoV-2 nucleocapsid RNA-binding domain

Abstract SARS-CoV-2 nucleocapsid (N) protein is a structural component of the virus with essential roles in the replication and packaging of the viral RNA genome. The N protein is also an important target of COVID-19 antigen tests and a promising vaccine candidate along with the spike protein. Here, we report a compact stem-loop DNA aptamer that binds tightly to the N-terminal RNA-binding domain of SARS-CoV-2 N protein. Crystallographic analysis shows that a hexanucleotide DNA motif (5′-TCGGAT-3′) of the aptamer fits into a positively charged concave surface of N-NTD and engages essential RNA-binding residues including Tyr109, which mediates a sequence-specific interaction in a uracil-binding pocket. Avid binding of the DNA aptamer allows isolation and sensitive detection of full-length N protein from crude cell lysates, demonstrating its selectivity and utility in biochemical applications. We further designed a chemically modified DNA aptamer and used it as a probe to examine the interaction of N-NTD with various RNA motifs, which revealed a strong preference for uridine-rich sequences. Our studies provide a high-affinity chemical probe for the SARS-CoV-2 N protein RNA-binding domain, which may be useful for diagnostic applications and investigating novel antiviral agents.

Biochemistry & Molecular Biology↗

Chloroplast lipid biosynthesis is fine-tuned to thylakoid membrane remodeling during light acclimation

Abstract Reprogramming metabolism, in addition to modifying the structure and function of the photosynthetic machinery, is crucial for plant acclimation to changing light conditions. One of the key acclimatory responses involves reorganization of the photosynthetic membrane system including changes in thylakoid stacking. Glycerolipids are the main structural component of thylakoids and their synthesis involves two main pathways localized in the plastid and the endoplasmic reticulum (ER); however, the role of lipid metabolism in light acclimation remains poorly understood. We found that fatty acid synthesis, membrane lipid content, the plastid lipid biosynthetic pathway activity, and the degree of thylakoid stacking were significantly higher in plants grown under low light compared with plants grown under normal light. Plants grown under high light, on the other hand, showed a lower rate of fatty acid synthesis, a higher fatty acid flux through the ER pathway, higher triacylglycerol content, and thylakoid membrane unstacking. We additionally demonstrated that changes in rates of fatty acid synthesis under different growth light conditions are due to post-translational regulation of the plastidic acetyl-CoA carboxylase activity. Furthermore, Arabidopsis mutants defective in one of the two glycerolipid biosynthetic pathways displayed altered growth patterns and a severely reduced ability to remodel thylakoid architecture, particularly under high light. Overall, this study reveals how plants fine-tune fatty acid and glycerolipid biosynthesis to cellular metabolic needs in response to long-term changes in light conditions, highlighting the importance of lipid metabolism in light acclimation.

59 BASIC BIOLOGICAL SCIENCES↗

Extending evolutionary forecasts across bacterial species

Improving evolutionary forecasting requires progressing from studying repeated evolution of a single genotype under identical conditions to formulating broad principles. These principles should enable predictions of how similar species will adapt to similar selective pressures. Evolve-and-resequence experiments with multiple species allow testing forecasts on different biological levels and elucidating the causes for failed predictions. Here, we show that forecasts for adaptation to static culture conditions can be extended to multiple species by testing previous predictions for Pseudomonas syringae and Pseudomonas savastanoi. In addition to sequence divergence, these species differ in their repertoire of biofilm regulatory genes and structural components. Consistent with predictions, both species repeatedly produced biofilm mutants with a wrinkly spreader phenotype. Predominantly, mutations occurred in the wsp operon, with less frequent promoter mutations near uncharacterized diguanylate cyclases. However, mutational patterns differed on the gene level, which was explained by a lack of conservation in relative fitness of mutants between more divergent species. The same mutation was the most frequent for both species suggesting that conserved mutation hotspots can increase parallel evolution. This study shows that evolutionary forecasts can be extended across species, but that differences in the genotype–phenotype–fitness map and mutational biases limit predictability on a detailed molecular level.

59 BASIC BIOLOGICAL SCIENCES↗