Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “water use”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 73 records · Page 4

Development of a two channel linear discriminant function for detecting and identifying surface water using ERTS-1 data

A method for detecting surface water using ERTS-1 multispectral scanner digital data is described. The several computer programs for processing multispectral data are described, along with the manner in which these programs were used. The use of the LARSAA program to test and demonstrate the discriminant function is explained. The discriminant function itself was later implemented in an operational program which performs only those specific operations necessary to test each data point for an indication of surface water in the scene. Several possible sources of difficulty in the use of the function are identified, and suggestions are made for avoiding or correcting those difficulties.

Source record↗

Constitutive down‐regulation of liguleless alleles in sorghum drives increased productivity and water use efficiency

Plant architecture influences the microenvironment throughout the canopy layer. Plants with a more erect leaf architecture allow for an increase in planting densities and allow more light to reach lower canopy leaves. This is predicted to increase crop carbon assimilation. Frictional resistance to wind reduces air movement in the lower canopy, resulting in higher humidity. By increasing the proportion of canopy photosynthesis in the more humid lower canopy, gains in the efficiency of water use might be expected, although this may be slightly offset by the more open erectophile form canopy. An anatomical feature in members of the Poaceae family that impacts leaf angle is the articulated junction of the sheath and blade, which also bares the ligule and auricles. Mutants, which lack ligules and auricles, show no articulation at this junction, resulting in leaves that are near vertical. In maize, these phenotypes termed liguleless result from null mutations of genes: ZmLG1 (Zm00001eb67740) and ZmLG2 (Zm00001eb147220). In sorghum, SbiRTx430.06G264300 (SbLG1) and SbiRTx430.03G392300 (SbLG2) are annotated as the respective maize homologues. A hair-pin element designed to down-regulate both SbLG1 and SbLG2 was introduced into the grain sorghum genotype RTx430. Derived transgenic events harbouring the hair-pin failed to develop ligules and displayed reduced leaf angles to the vertical, but less vertical than in null mutations. Under field settings, plots sown with these sorghum events having an erect architecture phenotype displayed an increase in photosynthesis in lower canopy levels, which led to increases in above-ground biomass and seed yield, without an increase in water use.

59 BASIC BIOLOGICAL SCIENCES↗

Data for "Constitutive Down-Regulation of Liguleless Alleles in Sorghum Drives Increased Productivity and Water Use Efficiency"

Plant architecture influences the microenvironment throughout the canopy layer. Plants with a more erect leaf architecture allow for an increase in planting densities and allow more light to reach lower canopy leaves. This is predicted to increase crop carbon assimilation. Frictional resistance to wind reduces air movement in the lower canopy, resulting in higher humidity. By increasing the proportion of canopy photosynthesis in the more humid lower canopy, gains in the efficiency of water use might be expected, although this may be slightly offset by the more open erectophile form canopy. An anatomical feature in members of the Poaceae family that impacts leaf angle is the articulated junction of the sheath and blade, which also bares the ligule and auricles. Mutants, which lack ligules and auricles, show no articulation at this junction, resulting in leaves that are near vertical. In maize, these phenotypes termed liguleless result from null mutations of genes: ZmLG1 (Zm00001eb67740) and ZmLG2 (Zm00001eb147220). In sorghum, SbiRTx430.06G264300 (SbLG1) and SbiRTx430.03G392300 (SbLG2) are annotated as the respective maize homologues. A hair-pin element designed to down-regulate both SbLG1 and SbLG2 was introduced into the grain sorghum genotype RTx430. Derived transgenic events harbouring the hair-pin failed to develop ligules and displayed reduced leaf angles to the vertical, but less vertical than in null mutations. Under field settings, plots sown with these sorghum events having an erect architecture phenotype displayed an increase in photosynthesis in lower canopy levels, which led to increases in above-ground biomass and seed yield, without an increase in water use.

Genome Engineering↗

Preserving isohydricity: vertical environmental variability explains Amazon forest water-use strategies

Abstract Increases in hydrological extremes, including drought, are expected for Amazon forests. A fundamental challenge for predicting forest responses lies in identifying ecological strategies which underlie such responses. Characterization of species-specific hydraulic strategies for regulating water-use, thought to be arrayed along an ‘isohydric–anisohydric’ spectrum, is a widely used approach. However, recent studies have questioned the usefulness of this classification scheme, because its metrics are strongly influenced by environments, and hence can lead to divergent classifications even within the same species. Here, we propose an alternative approach positing that individual hydraulic regulation strategies emerge from the interaction of environments with traits. Specifically, we hypothesize that the vertical forest profile represents a key gradient in drought-related environments (atmospheric vapor pressure deficit, soil water availability) that drives divergent tree water-use strategies for coordinated regulation of stomatal conductance (gs) and leaf water potentials (ΨL) with tree rooting depth, a proxy for water availability. Testing this hypothesis in a seasonal eastern Amazon forest in Brazil, we found that hydraulic strategies indeed depend on height-associated environments. Upper canopy trees, experiencing high vapor pressure deficit (VPD), but stable soil water access through deep rooting, exhibited isohydric strategies, defined by little seasonal change in the diurnal pattern of gs and steady seasonal minimum ΨL. In contrast, understory trees, exposed to less variable VPD but highly variable soil water availability, exhibited anisohydric strategies, with fluctuations in diurnal gs that increased in the dry season along with increasing variation in ΨL. Our finding that canopy height structures the coordination between drought-related environmental stressors and hydraulic traits provides a basis for preserving the applicability of the isohydric-to-anisohydric spectrum, which we show here may consistently emerge from environmental context. Our work highlights the importance of understanding how environmental heterogeneity structures forest responses to climate change, providing a mechanistic basis for improving models of tropical ecosystems.

Forestry↗

Assimilation of Remotely Sensed Leaf Area Index Enhances the Estimation of Anthropogenic Irrigation Water Use

Representation of irrigation in Earth System Models has advanced over the past decade, yet large uncertainties persist in the effective simulation of irrigation practices, particularly over locations where the on-ground practices and climate impacts are less reliably known. Here we investigate the utility of assimilating remotely sensed vegetation data for improving irrigation water use and associated fluxes within a land surface model. We show that assimilating optical sensor-based leaf area index estimates significantly improves the simulation of irrigation water use when compared to the USGS ground reports. For heavily irrigated areas, assimilation improves the evaporative fluxes and gross primary production (GPP) simulations, with the median correlation increasing by 0.1–1.1 and 0.3–0.6, respectively, as compared to the reference datasets. Further, bias improvements in the range of 14–35 mm mo^(−1) and 10–82 g m^(-2)mo^(−1) are obtained in evaporative fluxes and GPP as a result of incorporating vegetation constraints, respectively. These results demonstrate that the use of remotely sensed vegetation data is an effective, observation-informed, globally applicable approach for simulating irrigation and characterizing its impacts on water and carbon states.

Earth System Models↗

Drivers of Natural Variation in Water-Use Efficiency Under Fluctuating Light Are Promising Targets for Improvement in Sorghum

Improving leaf intrinsic water-use efficiency ( iWUE ), the ratio of photosynthetic CO 2 assimilation to stomatal conductance, could decrease crop freshwater consumption. iWUE has primarily been studied under steady-state light, but light in crop stands rapidly fluctuates. Leaf responses to these fluctuations substantially affect overall plant performance. Notably, photosynthesis responds faster than stomata to decreases in light intensity: this desynchronization results in substantial loss of iWUE . Traits that could improve iWUE under fluctuating light, such as faster stomatal movement to better synchronize stomata with photosynthesis, show significant natural diversity in C 3 species. However, C 4 crops have been less closely investigated. Additionally, while modification of photosynthetic or stomatal traits independent of one another will theoretically have a proportionate effect on iWUE , in reality these traits are inter-dependent. It is unclear how interactions between photosynthesis and stomata affect natural diversity in iWUE , and whether some traits are more tractable drivers to improve iWUE . Here, measurements of photosynthesis, stomatal conductance and iWUE under steady-state and fluctuating light, along with stomatal patterning, were obtained in 18 field-grown accessions of the C 4 crop sorghum. These traits showed significant natural diversity but were highly correlated, with important implications for improvement of iWUE . Some features, such as gradual responses of photosynthesis to decreases in light, appeared promising for improvement of iWUE . Other traits showed tradeoffs that negated benefits to iWUE , e.g., accessions with faster stomatal responses to decreases in light, expected to benefit iWUE , also displayed more abrupt losses in photosynthesis, resulting in overall lower iWUE . Genetic engineering might be needed to break these natural tradeoffs and achieve optimal trait combinations, e.g., leaves with fewer, smaller stomata, more sensitive to changes in photosynthesis. Traits describing iWUE at steady-state, and the change in iWUE following decreases in light, were important contributors to overall iWUE under fluctuating light.

54 ENVIRONMENTAL SCIENCES↗

Wood‐density has no effect on stomatal control of leaf‐level water use efficiency in an Amazonian forest

Forest disturbances increase the proportion of fast-growing tree species compared to slow-growing ones. To understand their relative capacity for carbon uptake and their vulnerability to climate change, and to represent those differences in Earth system models, it is necessary to characterise the physiological differences in their leaf-level control of water use efficiency and carbon assimilation. We used wood density as a proxy for the fast-slow growth spectrum and tested the assumption that trees with a low wood density (LWD) have a lower water-use efficiency than trees with a high wood density (HWD). We selected 5 LWD tree species and 5 HWD tree species growing in the same location in an Amazonian tropical forest and measured in situ steady-state gas exchange on top-of-canopy leaves with parallel sampling and measurement of leaf mass area and leaf nitrogen content. We found that LWD species invested more nitrogen in photosynthetic capacity than HWD species, had higher photosynthetic rates and higher stomatal conductance. Furthermore, contrary to expectations, we showed that the stomatal control of the balance between transpiration and carbon assimilation was similar in LWD and HWD species and that they had the same dark respiration rates.

54 ENVIRONMENTAL SCIENCES↗

A Technique for Remote Sensing of Suspended Sediments and Shallow Coastal Waters Using MODIS Visible and Near-IR Channels

We have developed an algorithm to detect suspended sediments and shallow coastal waters using imaging data acquired with the Moderate Resolution Imaging SpectroRadiometer (MODIS). The MODIS instruments on board the NASA Terra and Aqua Spacecrafts are equipped with one set of narrow channels located in a wide 0.4 - 2.5 micron spectral range. These channels were designed primarily for remote sensing of the land surface and atmosphere. We have found that the set of land and cloud channels are also quite useful for remote sensing of the bright coastal waters. We have developed an empirical algorithm, which uses the narrow MODIS channels in this wide spectral range, for identifying areas with suspended sediments in turbid waters and shallow waters with bottom reflections. In our algorithm, we take advantage of the strong water absorption at wavelengths longer than 1 micron that does not allow illumination of sediments in the water or a shallow ocean floor. MODIS data acquired over the east coast of China, west coast of Africa, Arabian Sea, Mississippi Delta, and west coast of Florida are used in this study.

Li, Rong-Rong↗

Evaluating ecosystem water use efficiency and recovery dynamics during flash droughts: insights from observations and model simulations

Flash droughts (FD), rapidly emerging in a warming future, disrupt ecosystems, agriculture, and water security. Ecosystem water use efficiency (WUE), the ratio of gross primary production (GPP) to actual evapotranspiration (AET), balances carbon assimilation and water loss. FD rapidly disrupts this balance, making WUE critical for assessing plant stress and recovery. Here, this study investigates the dynamics of landscape-scale WUE, and the components of GPP and AET under FD utilizing both observed data from the Missouri Ozark AmeriFlux site (US-MOz) and version 2 of the U.S. Department of Energy’s Earth, Energy, Exascale System Model (E3SM) Land Model (ELMv2). Observations and simulations reveal GPP as dominant for WUE during earlier FD events (2005, 2007, 2012), shifting to AET in recent events (2014, 2018). This agreement indicates that the ELM can capture the shifting dynamics of GPP and AET in regulating WUE under FD conditions. However, the ELM systematically underestimates both GPP and AET and does so in a manner that does not preserve their ratio. As a result, WUE is also underestimated, suggesting that GPP is more strongly underestimated than AET. Furthermore, the ELM also underestimates the speed of GPP recovery, producing an artificially prolonged GPP recovery time following FD events. Observed environmental drivers such as vapor pressure deficit (VPD), soil moisture (SM), and predawn leaf water potential (PLWP) effectively predict WUE, but ELM primarily highlights SM, underestimating VPD’s role. This study demonstrates that relying solely on soil moisture fails to capture the rapid hydraulic recovery observed in PLWP, underscoring the necessity of integrating plant hydraulics into land surface models to improve flash drought predictability.

Evapotranspiration↗

Estimating irrigation water use from remotely sensed evapotranspiration data: Accuracy and uncertainties at field, water right, and regional scales

Irrigated agriculture is the dominant user of water globally, but most water withdrawals are not monitored or reported. As a result, it is largely unknown when, where, and how much water is used for irrigation. Here, we evaluated the ability of remotely sensed evapotranspiration (ET) data, integrated with other datasets, to calculate irrigation water withdrawals and applications in an intensively irrigated portion of the United States. We compared irrigation calculations based on an ensemble of satellite-driven ET models from OpenET with reported groundwater withdrawals from hundreds of farmer irrigation application records and a statewide flowmeter database at three spatial scales (field, water right group, and management area). At the field scale, we found that ET-based calculations of irrigation agreed best with reported irrigation when the OpenET ensemble mean was aggregated to the growing season timescale (bias = 1.6–4.9%, R 2 = 0.53–0.74), and agreement between calculated and reported irrigation was better for multi-year averages than for individual years. At the water right group scale, linking pumping wells to specific irrigated fields was the primary source of uncertainty. At the management area scale, calculated irrigation exhibited similar temporal patterns as flowmeter data but tended to be positively biased with more interannual variability. Disagreement between calculated and reported irrigation was strongly correlated with annual precipitation, and calculated and reported irrigation agreed more closely after statistically adjusting for annual precipitation. The selection of an ET model was also an important consideration, as variability across ET models was larger than the potential impacts of conservation measures employed in the region. From these results, we suggest key practices for working with ET-based irrigation data that include accurately accounting for changes in soil moisture, deep percolation, and runoff; careful verification of irrigated area and well-field linkages; and conducting application-specific evaluations of uncertainty.

59 BASIC BIOLOGICAL SCIENCES↗

Evaluating ecosystem water use efficiency under drought stress: a case study of the Helan Mountain region, northwest China

Context Water use efficiency (WUE) is a fundamental ecological indicator links carbon assimilation and water loss in terrestrial ecosystems. Understanding its responses to drought stress is essential for adaptive ecosystem management, particularly in climate-sensitive mountain landscapes. Objectives This study aimed to investigate drought-driven variations in WUE across major vegetation types in the Helan Mountain region of Northwest China. Specifically, we sought to identify dominant ecological drivers of WUE variability and to disentangle their relative importance and causal pathways. Methods We quantified WUE using the Moderate Resolution Imaging Spectroradiometer (MODIS) products and the Drought Severity Index (DSI) data from 2001 to 2020. To examine WUE – drought relationships across contrasting vegetation types, we employed a spatially explicit analytical framework integrating Random Forest (RF) modeling, partial correlation analysis, and structural equation modeling (SEM). Results Regional WUE exhibited relatively stable interannual dynamics, yet pronounced spatial heterogeneity that was strongly modulated by drought conditions. Vegetation properties, particularly Leaf Area Index (LAI) and Normalized Difference Vegetation Index (NDVI), emerged as the dominant determinants of WUE, with NDVI alone explaining over 20% of its spatial variance in forest and grassland during non-drought periods. SEM analyses revealed that climate forcing influenced WUE mainly through indirect pathways mediated by soil moisture availability and vegetation structural dynamics, rather than through direct climatic controls. Among all regulating factors, LAI acted as the central control node governing ecosystem carbon–water coupling. In contrast, short-term climatic stress, especially atmospheric demand and drought duration, exerted weak or negative direct effects on WUE. Ecosystem-specific responses were observed, with croplands mainly regulated by soil water availability, whereas forests and grasslands showed more sensitive to atmospheric drought stress. Together, these results reveal a hierarchical control framework where soil–vegetation interactions mediate climate impacts on WUE, driving strong spatial heterogeneity in drought responses across mountain landscapes. Conclusions Our findings highlight the pivotal role of indirect drought effects mediated by vegetation and soil processes in shaping ecosystem WUE. The identified soil–vegetation–climate regulatory hierarchy provides mechanistic insight into landscape–scale drought sensitivity and supports integrated modeling approaches for evaluating ecosystem resilience and sustainable management in arid mountain regions.

China↗

Hourly water-carbon interactions modulate decadal water-use efficiency trends inferred from ecosystem-scale measurements

Plant stomatal conductance regulates photosynthesis and transpiration. This physiological link affects ecosystem responses to microclimate and harmonizes carbon, energy, and water exchanges between the biosphere and atmosphere. The relationship between water losses via transpiration and carbon gains via photosynthesis can be quantified by plant water-use efficiency (WUE). While leaf- and ecosystem-scale observations both suggest rising WUE in recent decades, WUE trends inferred from the ecosystem scale are much larger than those inferred from the leaf scale or implied by theory. The unexpectedly large ecosystem-scale WUE trends complicate interpretation of ecophysiological responses to changing environmental conditions. Here, we analyze ecosystem-scale WUE inferred from 40 FLUXNET sites, each with at least 10 years of measurements. Furthermore, our results demonstrate that observed ecosystem-scale WUE trends are more sensitive to hourly weather conditions than longer-term changes in atmospheric carbon dioxide or vapor pressure deficit. Our analysis shows that Earth System Models participating in CMIP6 did not capture the observed WUE sensitivity to inter-site variability and microclimatic conditions. Collectively, our findings suggest that ecosystem-scale WUE trends reflect water-carbon interactions across multiple temporal scales, and disentangling factors contributing to emergent ecosystem responses is needed to infer ecophysiological relationships and model structures from observations.

54 ENVIRONMENTAL SCIENCES↗

Analysis of gas turbine engines using water and oxygen injection to achieve high Mach numbers and high thrust

An analysis of gas turbine engines using water and oxygen injection to enhance performance by increasing Mach number capability and by increasing thrust is described. The liquids are injected, either separately or together, into the subsonic diffuser ahead of the engine compressor. A turbojet engine and a mixed-flow turbofan engine (MFTF) are examined, and in pursuit of maximum thrust, both engines are fitted with afterburners. The results indicate that water injection alone can extend the performance envelope of both engine types by one and one-half Mach numbers at which point water-air ratios reach 17 or 18 percent and liquid specific impulse is reduced to some 390 to 470 seconds, a level about equal to the impulse of a high energy rocket engine. The envelope can be further extended, but only with increasing sacrifices in liquid specific impulse. Oxygen-airflow ratios as high as 15 percent were investigated for increasing thrust. Using 15 percent oxygen in combination with water injection at high supersonic Mach numbers resulted in thrust augmentation as high as 76 percent without any significant decrease in liquid specific impulse. The stoichiometric afterburner exit temperature increased with increasing oxygen flow, reaching 4822 deg R in the turbojet engine at a Mach number of 3.5. At the transonic Mach number of 0.95 where no water injection is needed, an oxygen-air ratio of 15 percent increased thrust by some 55 percent in both engines, along with a decrease in liquid specific impulse of 62 percent. Afterburner temperature was approximately 4700 deg R at this high thrust condition. Water and/or oxygen injection are simple and straightforward strategies to improve engine performance and they will add little to engine weight. However, if large Mach number and thrust increases are required, liquid flows become significant, so that operation at these conditions will necessarily be of short duration.

Henneberry, Hugh M.↗

Deep learning techniques to disentangle water use efficiency, climate change, and carbon sequestration across ecosystem scales

Focal Areas: We plan to use machine learning (ML) to disentangle the impact of climate change on plant water use efficiency (WUE) across the leaf level, structured canopy, and community ecosystem scales. Due to the complex behavior of modeling plant WUE’s connection to carbon storage (and hidden co-varying interactions) we suggest that physics-based deep learning must be utilized. The second topic we propose is, based on a better understanding of WUE response to climate change, there is a need to predict how WUE will limit or enhance the recycling of water from transpiration by vegetation back to the land surface worldwide, and thus impact water storage capacity (i.e., natural and reservoirs), using machine learning optimization techniques such as genetic algorithms.

54 ENVIRONMENTAL SCIENCES↗

High Vapor Pressure Deficit Decreases the Productivity and Water Use Efficiency of Rain‐Induced Pulses in Semiarid Ecosystems

Abstract Intermittent rain events drive dynamic pulses of carbon and water exchange in many arid and semiarid ecosystems. Although soil moisture is known to control these pulses, the effect of atmospheric dryness on pulses is not well documented. Here we hypothesized that vapor pressure deficit ( VPD ) modulates net ecosystem production ( NEP ) and ecosystem‐scale water use efficiency ( WUE ) during pulse events due to its effects on canopy stomatal conductance and evapotranspiration. We quantified relationships between VPD and carbon and water exchange during growing season rain events and tested their generality across four semiarid flux sites with varied vegetation in the southwest United States. Across grassland, shrubland, and savanna sites, we found that high VPD during pulses suppressed ecosystem photosynthesis and surface conductance to a greater degree than respiration or evapotranspiration, particularly when soil moisture was high. Thus, periods of high VPD were associated with a 13–64% reduction in NEP and an 11–25% decrease in WUE , relative to moderate VPD conditions. Sites dominated by shrubs with the C3 photosynthetic pathway were more sensitive to VPD than sites dominated by C4 grasses. We found that a 1 kPa increase in VPD reduced the average NEP of pulse events by 13–56%, which illustrates the potential for projected increases in atmospheric demand to reduce the net productivity of semiarid ecosystems.

Roby, Matthew C.↗

Agricultural impacts of sustainable water use in the United States

Abstract Governance measures such as restrictions on groundwater pumping and adjustments to sectoral water pricing have been suggested as response strategies to curtail recent increases in groundwater pumping and enhance sustainable water use. However, little is known about the impacts of such sustainability strategies. We investigate the implications of such measures, with the United States (U.S.) as an example. Using the Global Change Analysis Model (GCAM) with state-level details in the U.S., we find that the combination of these two governance measures can drastically alter agricultural production in the U.S. The Southwest stands to lose upwards of 25% of their total agricultural production, much of which is compensated for by production increases in river basins on the east coast of the U.S. The implementation of future sustainable water governance measures will require additional investments that allow farmers to maximize production while minimizing water withdrawals to avoid potentially detrimental revenue losses.

54 ENVIRONMENTAL SCIENCES↗

Mine Water Use, Treatment, and Reuse in the United States: A Look at Current Industry Practices and Select Case Studies

Mining provides significant economic value while often impacting local water supplies and environments because of freshwater usage and waste disposal practices. In this paper we identify current practices in mine water, including how water is used in mining, influent and effluent water quality, treatment technologies, and end uses with the goal of informing future research on implementable, reliable, and cost-effective advanced water treatment in the mining sector. This study also reviews the available literature to broadly evaluate mining in the United States and performs a techno-economic assessment on water use and disposal for three detailed case studies applicable to lithium, uranium, and copper mines. These case studies highlight specific industry examples of distinct extraction methods, geographical regions, and mined commodities. Hypothetical scenarios based on case study baselines revealed potential impacts to mine water available for beneficial reuse through the use of novel water treatment technologies and alternate water management strategies. Finally, an assessment of national level impacts resulting from the reuse of treated mine source water is presented.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Phenotyping stomatal closure by thermal imaging for GWAS and TWAS of water use efficiency-related genes

Abstract Stomata allow CO2 uptake by leaves for photosynthetic assimilation at the cost of water vapor loss to the atmosphere. The opening and closing of stomata in response to fluctuations in light intensity regulate CO2 and water fluxes and are essential for maintaining water-use efficiency (WUE). However, a little is known about the genetic basis for natural variation in stomatal movement, especially in C4 crops. This is partly because the stomatal response to a change in light intensity is difficult to measure at the scale required for association studies. Here, we used high-throughput thermal imaging to bypass the phenotyping bottleneck and assess 10 traits describing stomatal conductance (gs) before, during and after a stepwise decrease in light intensity for a diversity panel of 659 sorghum (Sorghum bicolor) accessions. Results from thermal imaging significantly correlated with photosynthetic gas exchange measurements. gs traits varied substantially across the population and were moderately heritable (h2 up to 0.72). An integrated genome-wide and transcriptome-wide association study identified candidate genes putatively driving variation in stomatal conductance traits. Of the 239 unique candidate genes identified with the greatest confidence, 77 were putative orthologs of Arabidopsis (Arabidopsis thaliana) genes related to functions implicated in WUE, including stomatal opening/closing (24 genes), stomatal/epidermal cell development (35 genes), leaf/vasculature development (12 genes), or chlorophyll metabolism/photosynthesis (8 genes). These findings demonstrate an approach to finding genotype-to-phenotype relationships for a challenging trait as well as candidate genes for further investigation of the genetic basis of WUE in a model C4 grass for bioenergy, food, and forage production.

54 ENVIRONMENTAL SCIENCES↗