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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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61 records · Page 4
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

The SrrAB two-component system regulates Staphylococcus aureus pathogenicity through redox sensitive cysteines

Staphylococcus aureus infections can lead to diseases that range from localized skin abscess to life-threatening toxic shock syndrome. The SrrAB two-component system (TCS) is a global regulator of S. aureus virulence and critical for survival under environmental conditions such as hypoxic, oxidative, and nitrosative stress found at sites of infection. Despite the critical role of SrrAB in S. aureus pathogenicity, the mechanism by which the SrrAB TCS senses and responds to these environmental signals remains unknown. Bioinformatics analysis showed that the SrrB histidine kinase contains several domains, including an extracellular Cache domain and a cytoplasmic HAMP-PAS-DHp-CA region. In this work, we show that the PAS domain regulates both kinase and phosphatase enzyme activity of SrrB and present the structure of the DHp-CA catalytic core. Importantly, this structure shows a unique intramolecular cysteine disulfide bond in the ATP-binding domain that significantly affects autophosphorylation kinetics. In vitro data show that the redox state of the disulfide bond affects S. aureus biofilm formation and toxic shock syndrome toxin-1 production. Moreover, with the use of the rabbit infective endocarditis model, we demonstrate that the disulfide bond is a critical regulatory element of SrrB function during S. aureus infection. Our data support a model whereby the disulfide bond and PAS domain of SrrB sense and respond to the cellular redox environment to regulate S. aureus survival and pathogenesis.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Estimating Explosion Yields Using Moment Tensor Solutions and Seismic Moment

We report seismic moment, a measurable and well-understood quantity of seismic sources, is used to estimate the yield of explosions. Application of such a method in the past, as in the manner of m b -derived yields, has been complicated by the effect of variations in the explosion working point, depth, and secondary source effects (such as spalling and tectonic release) on the observed moment. We start using the full (six-element) moment tensor solution, which can capture the relevant source physics and, at least in theory, better isolate the primary explosion source. The moment-to-yield ratio is then estimated using an explosion source model which, provided with emplacement conditions, can relate the two parameters. We discuss the major sources of uncertainty associated with the method, and calibrate it with chemical and nuclear explosions at the Nevada National Security Site. We then apply the method to published moment tensor solutions for the six declared North Korean nuclear explosions that occurred between 2006 and 2017. The results are mostly consistent with other yield estimates made using a variety of high-frequency methods. This technique is a new approach to estimating explosive yield and simple to implement, as much of the complexity is captured by the source models.

58 GEOSCIENCES↗