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At least 73 records · Page 4

Construction and Modeling of a Coculture Microplate for Real-Time Measurement of Microbial Interactions

The dynamic structures of microbial communities emerge from the complex network of interactions between their constituent microorganisms. Quantitative measurements of these interactions are important for understanding and engineering ecosystem structure. Here, we present the development and application of the BioMe plate, a redesigned microplate device in which pairs of wells are separated by porous membranes. BioMe facilitates the measurement of dynamic microbial interactions and integrates easily with standard laboratory equipment. We first applied BioMe to recapitulate recently characterized, natural symbiotic interactions between bacteria isolated from the Drosophila melanogaster gut microbiome. Specifically, the BioMe plate allowed us to observe the benefit provided by two Lactobacillus strains to an Acetobacter strain. We next explored the use of BioMe to gain quantitative insight into the engineered obligate syntrophic interaction between a pair of Escherichia coli amino acid auxotrophs. We integrated experimental observations with a mechanistic computational model to quantify key parameters associated with this syntrophic interaction, including metabolite secretion and diffusion rates. This model also allowed us to explain the slow growth observed for auxotrophs growing in adjacent wells by demonstrating that, under the relevant range of parameters, local exchange between auxotrophs is essential for efficient growth. The BioMe plate provides a scalable and flexible approach for the study of dynamic microbial interactions.

3D printed device↗

Real-time Measurements of Complex Transition Metal Oxide Nanostructure Growth (Year 3 - Final Technical Report-GaTech-MIT)

The project, a collaboration between the Ross Lab at MIT and the Filler Lab at Georgia Tech, aimed to combine in situ microscopy and spectroscopy measurements to answer fundamental questions about the physics and chemistry governing the bottom-up vapor-solid-liquid (VLS) growth of one-dimensional (1-D) functional oxides. This work supported one PhD student and 1 postdoc. One paper has been published and 5 others are in preparation. To date, this work has been presented at 5 conferences.

36 MATERIALS SCIENCE↗

Autoignition Delay Time Measurements of Dimethyl Ether at 110 Bar Inside a Shock Tube

Fuel mixtures of propane and dimethyl ether have been proposed as potential diesel substitutes for use in compression-ignition engines. There is an interest in phasing out diesel fuel because its combustion byproducts are harmful to the environment. Propane and dimethyl ether burn cleaner and produce significantly less carbon dioxide than diesel. The combustion of these fuels at engine-relevant conditions must be characterized to create an engine capable of running on propane and dimethyl ether. In this work, IDT measurements of stoichiometric neat DME diluted in 75% argon were completed for temperatures spanning 800-1200K at 110 bar. A shock tube will be used for all experiments in this work. Experimental IDTs were then compared to simulated values using several well-established mechanisms in the literature.

Guenther, Aaron C. [University of Central Florida]↗

Delay time measurements of the propagation of radio waves in the atmosphere

The characteristics and operation of the Geodetic Secor System are described. The precision of the ionospheric radiation measurements was determined by a collocation experiment. The EGRS-13 satellite, which was used in the experiment, is discussed. The geodetic network is shown in a diagram form. Conclusions resulting from the experiments are reported.

Rohde, F.↗

On the use of measured time delays in gravitational lenses to determine the Hubble constant

Gravitational lenses are rare in the known samples of quasars, indicating that the conditions involved in their formation are unusual. In particular, the distribution of matter along the light rays from the observer through the deflector to the quasar may be very different from mean conditions. It is shown that reasonable deviations in the density of matter along the beams can significantly alter the relationship between time delays and the Hubble constant, and it is concluded that gravitational lenses are not promising estimators of this constant. However, should an independent, precise determination of the Hubble constant become available, gravitational lenses could be used to probe long-range density fluctuations.

Alcock, C.↗

A Waveform Skewness Index for Measuring Time Series Nonlinearity and Its Applications to the ENSO-Indian Monsoon Relationship

Many geophysical time series possess nonlinear characteristics that reflect the underlying physics of the phenomena the time series describe. The nonlinear character of times series can change with time, so it is important to quantify time series nonlinearity without assuming stationarity. A common way of quantifying the time evolution of time series nonlinearity is to compute sliding skewness time series, but it is shown here that such an approach can be misleading when time series contain periodicities. To remedy this deficiency of skewness, a new waveform skewness index is proposed for quantifying local nonlinearities embedded in time series. A waveform skewness spectrum is proposed for determining the frequency components that are contributing to time series waveform skewness. The new methods are applied to the El Niño–Southern Oscillation (ENSO) and the Indian monsoon to test a recently proposed hypothesis that states that changes in the ENSO–Indian monsoon relationship are related to ENSO nonlinearity. We show that the ENSO–Indian rainfall relationship weakens during time periods of high ENSO waveform skewness. The results from two different analyses suggest that the breakdown of the ENSO–Indian monsoon relationship during time periods of high ENSO waveform skewness is related to the more frequent occurrence of strong central Pacific El Niño events, supporting arguments that changes in the ENSO–Indian rainfall relationship are not solely related to noise.

Justin Schulte↗