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Results for “mechanical activation”
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A structure-function analysis of chlorophyllase reveals a mechanism for activity regulation dependent on disulfide bonds
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Physical basis for distinct basal and mechanically gated activity of the human K[superscript +] chan
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Combining Kinetics and Operando Spectroscopy to Interrogate the Mechanism and Active Site Requiremen
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Mechanisms of activation and desensitization of full-length glycine receptor in lipid nanodiscs
Glycinergic synapses play a central role in motor control and pain processing in the central nervous system. Glycine receptors (GlyRs) are key players in mediating fast inhibitory neurotransmission at these synapses. While previous high-resolution structures have provided insights into the molecular architecture of GlyR, several mechanistic questions pertaining to channel function are still unanswered. Here, we present Cryo-EM structures of the full-length GlyR protein complex reconstituted into lipid nanodiscs that are captured in the unliganded (closed), glycine-bound (open and desensitized), and allosteric modulator-bound conformations. A comparison of these states reveals global conformational changes underlying GlyR channel gating and modulation. The functional state assignments were validated by molecular dynamics simulations, and the observed permeation events are in agreement with the anion selectivity and conductance of GlyR. These studies provide the structural basis for gating, ion selectivity, and single-channel conductance properties of GlyR in a lipid environment.
Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA
Cas7-11 is a Type III-E CRISPR Cas effector that confers programmable RNA cleavage and has potential applications in RNA interference. Cas7-11 encodes a single polypeptide containing four Cas7- and one Cas11-like segments that obscures the distinction between the multi-subunit Class 1 and the single-subunit Class-2 CRISPR Cas systems. We report a cryo-EM (cryo-electron microscopy) structure of the active Cas7-11 from Desulfonema ishimotonii (DiCas7-11) that reveals the molecular basis for RNA processing and interference activities. DiCas7-11 arranges its Cas7- and Cas11-like domains in an extended form that resembles the backbone made up by four Cas7 and one Cas11 subunits in the multi-subunit enzymes. Unlike the multi-subunit enzymes, however, the backbone of DiCas7-11 contains evolutionarily different Cas7 and Cas11 domains, giving rise to their unique functionality. The first Cas7-like domain nearly engulfs the last 15 direct repeat nucleotides in processing and recognition of the CRISPR RNA, and its free-standing fragment retains most of the activity. Both the second and the third Cas7-like domains mediate target RNA cleavage in a metal-dependent manner. The structure and mutational data indicate that the long variable insertion to the fourth Cas7 domain has little impact on RNA processing or targeting, suggesting the possibility for engineering a compact and programmable RNA interference tool.
On the hard X-ray emission mechanism of active galactic nuclei sources
Within the framework of unsaturated Compton disk accretion onto a supermassive black hole as a model for power-law active-galactic-nuclei X-ray sources (as opposed to the synchro-Compton model), the hot-inner-disk model of Shapiro, Lightman, and Eardley (1976) is compared with the disk-corona model with balanced conduction and Compton losses. Both can generate electron temperatures of at least about 1 billion K in the supermassive case but promise other observable distinctions. The sandwich configuration of the disk corona provides a natural explanation of why Comptonization is unsaturated.
The Penrose photoproduction scenario for NGC 4151: A black hole gamma-ray emission mechanism for active galactic nuclei and Seyfert galaxies
A consistent theoretical interpretation is given for the suggestion that a steepening of the spectrum between X-ray and gamma ray energies may be a general, gamma-ray characteristic of Seyfert galaxies, if the diffuse gamma ray spectrum is considered to be a superposition of unresolved contributions, from one or more classes of extragalactic objects. In the case of NGC 4151, the dominant process is shown to be Penrose Compton scattering in the ergosphere of a Kerr black hole, assumed to exist in the Seyfert's active galactic nucleus.
The Penrose Photoproduction Scenario for NGC 4151 /PCS-SSC/ - A black hole gamma-ray emission mechanism for active galactic nuclei and Seyfert galaxies
On the basis of general arguments, it has been suggested (Bignami et al., 1979) that a steepening of the spectrum between X-ray and gamma-ray energies may be a general, gamma-ray characteristic of Seyfert galaxies, if the diffuse gamma-ray spectrum is considered to be a superposition of unresolved contributions, from one or more classes of extragalactic objects. It is shown that the above suggestion can be given a consistent theoretical interpretation in the context of the Penrose Photoproduction Scenario (PCS-SSC). Specifically in the case of NGC 4151, the dominant process will be shown to be Penrose Compton Scattering PCS in the ergosphere of a massive, central, rapidly spinning Kerr black hole, assumed in its nucleus. It is assumed that PCS gamma-ray induced electron-position, Synchrotron-Self-Compton processes 'evolve' as the dominant source of nonthermal X-rays.
Benefits of detailed models of muscle activation and mechanics
Recent biophysical and physiological studies identified some of the detailed mechanisms involved in excitation-contraction coupling, muscle contraction, and deactivation. Mathematical models incorporating these mechanisms allow independent estimates of key parameters, direct interplay between basic muscle research and the study of motor control, and realistic model behaviors, some of which are not accessible to previous, simpler, models. The existence of previously unmodeled behaviors has important implications for strategies of motor control and identification of neural signals. New developments in the analysis of differential equations make the more detailed models feasible for simulation in realistic experimental situations.
Mechanisms of active control in cylindrical fuselage structures
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Winner-take-all networks - Time-based versus activation-based mechanisms for various selection goals
As an alternative to conventional winner-take-all mechanisms, the paper presents a temporal winner-take-all mecahnism with O(n) space complexity and roughly O(log n) time complexity. It exploits systematic and stochastic differences between time delays within different units and connections.
Interplanetary Stock Triggering of Auroral Substorm Activity: A Mechanism
We use 1997-1998 Wind Solar Wind data and POLAR UV imaging data to study magnetospheric responses and substorm triggering mechanisms during and after interplanetary (IP) shock events.
Interplanetary Shock Triggering of Auroral Substorm Activity: A Mechanism
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Active mechanical-environmental-thermal MEMS device for nanoscale characterization
A microelectromechanical system (MEMS) device can be used for quantitative mechanical testing of materials within a controlled (chemical and temperature) environment, with the ability for electrochemical control to the specimen, that is coupled with a complimentary in-situ characterization technique.
Continental rifting - Progress and outlook
It is noted that in spite of the flood of new data on continental rifts in the last 15 years, there is little consensus about the basic mechanisms and causes of rifting. The remarkable similarities in rift cross sections (shown in a figure), are considered to suggest that the anomalous lithospheric structure of rifts is more dependent on lithosphere properties than the mode of rifting. It is thought that there is a spectrum of rifting processes for which two fundamental mechanisms can be postulated: an active mechanism, whereby thermal energy is transmitted into the lithosphere from the underlying asthenosphere, and a passive mechanism by which mechanical energy is transmitted laterally through the lithosphere as a consequence of plate interactions at a distance. In order to permit the concept of the two fundamentally different mechanisms to be tested, a tentative classification is proposed that divides rifts into two basic categories: active rifting and passive rifting. Here, the magnitude of active rifting will depend on the rate at which lithosphere moves over the thermal source, with rifts being restricted to stationary or slow-moving plates.
Chapter 5: Methods for Algal Protein Isolation and Proteome Analysis
Microalgae present promising feedstocks to produce renewable fuel and chemical intermediates, in part due to high storage carbon flux capacity to triacylglycerides or storage carbohydrates upon nutrient deprivation. However, the mechanism(s) governing deprivation-mediated carbon partitioning remain to be fully elucidated, limiting targeted strain engineering strategies in algal biocatalysts. Though genomic and transcriptomic analyses offer key insights into these mechanisms, active post-transcriptional regulatory mechanisms, ubiquitous in many microalgae, necessitate proteomic and post-translational (e.g., phospho- and nitroso-proteomic) analyses to more completely evaluate algal responsiveness to nutrient deprivation. Herein, we describe methods for isolating total algal protein and conducting proteomic, phosphoproteomic, and nitrosoproteomic analyses. We focus on methods deployed for the chlorophyte, Chlorella vulgaris, a model oleaginous alga with high flux to renewable fuel and chemical precursors.