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At least 73 records · Page 4

Benchmarking second and third-generation sequencing platforms for microbial metagenomics

Shotgun metagenomic sequencing is a common approach for studying the taxonomic diversity and metabolic potential of complex microbial communities. Current methods primarily use second generation short read sequencing, yet advances in third generation long read technologies provide opportunities to overcome some of the limitations of short read sequencing. Here, we compared seven platforms, encompassing second generation sequencers (Illumina HiSeq 300, MGI DNBSEQ-G400 and DNBSEQ-T7, ThermoFisher Ion GeneStudio S5 and Ion Proton P1) and third generation sequencers (Oxford Nanopore Technologies MinION R9 and Pacific Biosciences Sequel II). We constructed three uneven synthetic microbial communities composed of up to 87 genomic microbial strains DNAs per mock, spanning 29 bacterial and archaeal phyla, and representing the most complex and diverse synthetic communities used for sequencing technology comparisons. Our results demonstrate that third generation sequencing have advantages over second generation platforms in analyzing complex microbial communities, but require careful sequencing library preparation for optimal quantitative metagenomic analysis. Our sequencing data also provides a valuable resource for testing and benchmarking bioinformatics software for metagenomics.

59 BASIC BIOLOGICAL SCIENCES↗

The Telecommunications and Data Acquisition Report

This quarterly publication provides archival reports on developments in programs managed by JPL's Telecommunications and Mission Operations Directorate (TMOD), which now includes the former Telecommunications and Data Acquisition (TDA) Office. In space communications, radio navigation, radio science, and ground-based radio and radar astronomy, it reports on activities of the Deep Space Network (DS) in planning, supporting research and technology, implementation, and operations. Also included are standards activity at JPL for space data and information systems and reimbursable DSN work performed for other space agencies through NASA. The preceding work is all performed for NASA's Office of Space Communications (OSC).

Yuen, Joseph H.↗

The Telecommunications and Data Acquisition Report

This quarterly publication provides archival reports on developments in programs managed by JPL's Telecommunications and Mission Operations Directorate (TMOD), which now includes the former Telecommunications and Data Acquisition (TDA) Office. In space communications, radio navigation, radio science, and ground-based radio and radar astronomy, it reports on activities of the Deep Space Network (DSN) in planning, supporting research and technology, implementation, and operations. Also included are standards activity at JPL for space data and information systems and reimbursable DSN work performed for other space agencies through NASA. The preceding work is all performed for NASA's Office of Space Communications (OSC).

Yuen, Joseph H.↗

The Telecommunications and Data Acquisition Report

This quarterly publication provides archival reports on developments in programs managed by JPL's Telecommunications and Mission Operations Directorate (TMOD), which now includes the former Telecommunications and Data Acquisition (TDA) Office. In space communications, radio navigation, radio science, and ground-based radio and radar astronomy, it reports on activities of the Deep Space Network (DSN) in planning, supporting research and technology, implementation, and operations. Also included are standards activity at JPL for space data and information systems and reimbursable DSN work performed for other space agencies through NASA. The preceding work is all performed for NASA's Office of Space Communications (OSC).

Yuen, Joseph H.↗

The Telecommunications and Data Acquisition Report

This quarterly publication provides archival reports on developments in programs managed by JPL's Telecommunications and Mission Operations Directorate (TMOD), which now includes the former Telecommunications and Data Acquisition (TDA) Office. In space communications, radio navigation, radio science, and ground-based radio and radar astronomy, it reports on activities of the Deep Space Network (DSN) in planning, supporting research and technology, implementation, and operations. Also included are standards activity at JPL for space data and information systems and reimbursable DSN work performed for other space agencies through NASA. The preceding work is all performed for NASA's Office of Space Communications (OSC). TMOD also performs work funded by other NASA program offices through and with the cooperation of OSC. The first of these is the Orbital Debris Radar Program funded by the Office of Space Systems Development. It exists at Goldstone only and makes use of the planetary radar capability when the antennas are configured as science instruments making direct observations of the planets, their satellites, and asteroids of our solar system. The Office of Space Sciences funds the data reduction and science analyses of data obtained by the Goldstone Solar System Radar. The antennas at all three complexes are also configured for radio astronomy research and, as such, conduct experiments funded by the National Science Foundation in the U.S. and other agencies at the overseas complexes. These experiments are either in microwave spectroscopy or very long baseline interferometry. Finally, tasks funded under the JPL Director's Discretionary Fund and the Caltech President's Fund that involve TMOD are included. This and each succeeding issue of 'The Telecommunications and Data Acquisition Progress Report' will present material in some, but not necessarily all, of the aforementioned programs.

Yuen, Joseph H.↗

New York City Panel on Climate Change 2019 Report Chapter 8: Indicators and Monitoring

The Indicators and Monitoring chapter of the first New York City Panel on Climate Change Report began with the paradigm: What cannot be measured cannot be managed (Rosenzweig et al., 2010). This statement is as valid today as it was then.The NPCC1 (2010) Indicators and Monitoring chapter addressed the need for assembling a suite of indicators to monitor climate change and adaptation in order to inform climate change decision making. It outlined criteria for selection of indicators (policy relevance, analytic soundness, measurability), defined categories of indicators (physical climate change; risk exposure, vulnerability, and impacts; adaptation; new research), and provided examples of specific indicators. Table 8.1 is a summary table of indicator development contribution from the NPCC1 I&M chapter (Jacob et al., 2011). The chapter explored the institutional requirements for indicator data availability, continuity, archiving, and public accessibility.

Blake, Reginald↗

NASA HECC Geometry and Performance Review Part 3: A Numerical and Experimental Investigation of Tip Clearance Effects on the Vaneless Diffuser Configuration

Tip clearance effects in centrifugal compressors have been extensively investigated to understand the losses associated with the flow in the impeller tip clearance gap between the rotating blades and the stationary shroud. In Part 3 of this multipart investigation, experimental data and validated numerical simulations from Parts 1 and 2 were used to analyze the effects of the size of the tip clearance gap on the High Efficiency Centrifugal Compressor performance and aerodynamics. Four tip clearance gaps ranging from 0.012-in to 0.030-in (2.0% to 4.9% of the impeller exit blade height) were considered at both design and off-design operating conditions. The total pressure ratio and efficiency of the stage are found to decay linearly with increasing tip gaps. The sensitivity of the impeller performance to the tip gap was found to vary with the rotational speed of the compressor. Spanwise surveys of flow angle, total pressure, and total temperature collected at the impeller exit at design speed are used to validate numerical simulations at each experimental tip gap condition. Numerical simulations show that increased turbulence kinetic energy near the shroud at larger tip gaps leads to spanwise mixing of high entropy fluid near the impeller trailing edge which decreases the useful work input by the impeller. The data presented have been made available to the public in the HECC Data Archive located at https://storage.googleapis.com/hecc-data/NASA-HECC-Data-Archive.zip.

centrifugal compressor↗

NASA HECC Geometry and Performance Review Part 3: A Numerical and Experimental Investigation of Tip Clearance Effects on the Vaneless Diffuser Configuration

Tip clearance effects in centrifugal compressors have been extensively investigated to understand the losses associated with the flow in the impeller tip clearance gap between the rotating blades and the stationary shroud. In Part 3 of this multipart investigation, experimental data and validated numerical simulations from Parts 1 and 2 were used to analyze the effects of the size of the tip clearance gap on the High Efficiency Centrifugal Compressor performance and aerodynamics. Four tip clearance gaps ranging from 0.012-in to 0.030-in (2.0% to 4.9% of the impeller exit blade height) were considered at both design and off-design operating conditions. The total pressure ratio and efficiency of the stage are found to decay linearly with increasing tip gaps. The sensitivity of the impeller performance to the tip gap was found to vary with the rotational speed of the compressor. Spanwise surveys of flow angle, total pressure, and total temperature collected at the impeller exit at design speed are used to validate numerical simulations at each experimental tip gap condition. Numerical simulations show that increased turbulence kinetic energy near the shroud at larger tip gaps leads to spanwise mixing of high entropy fluid near the impeller trailing edge which decreases the useful work input by the impeller. The data presented have been made available to the public in the HECC Data Archive located at https://storage.googleapis.com/hecc-data/NASA-HECC-Data-Archive.zip.

centrifugal compressor↗

Multi-Scale 3D Imaging for Machine Learning Property Upscaling: Mt. Simon Sandstone Case Study

Petrographic properties of principal target reservoirs for carbon sequestration, such as the Mt. Simon Sandstone, are relevant to broad interest groups. The Mt. Simon Sandstone is a deep, saline, regionally extensive Cambrian sandstone, overlain by low permeability sealing formations, making it one of the viable geologic carbon storage reservoirs in the Midwestern US. Its thickness (exceeding 2400 ft in some localities), depth, and lateral extent, combined with high porosity and permeability make it a high-priority target of multiple ongoing geologic carbon sequestration efforts in the United States of America. The National Energy Technology Laboratory in Morgantown, West Virginia, has been engaged in characterization efforts of the Mt. Simon for over a decade, with a strong focus on Computed Tomographic data acquisition. Data generated during this period has been hitherto not accessible to the public. This archival effort focused on preservation of historical CT data and associated metadata, and facilitating their accessibility, culminating with the publication of the entire dataset on NETL’s Energy Data eXchange (EDX) and the associated Gill et. al (2024) paper.

Gill, Magdalena K.↗

Transportation Secure Data Center: Frequently Asked Questions for Data Owners/Contributors

The Transportation Secure Data Center is a centralized repository for detailed transportation data from travel and transit surveys and studies conducted across the nation. It makes vital transportation data broadly available to users while preserving the privacy of survey participants. Hundreds of datasets from surveys and studies of household travel and transit passenger travel are archived in the TSDC, including surveys and studies conducted by state departments of transportation, metropolitan planning organizations, transit agencies, cities, and other public agencies. Detailed data from travel surveys and studies are extremely valuable for research purposes. However, the fine-grained information they contain could potentially be misused to identify individual travelers, so access to these data should only be granted with safeguards in place to protect participant privacy. The TSDC was created to address this challenge and to relieve public agencies from the burden of archiving their data and responding to data requests.

33 ADVANCED PROPULSION SYSTEMS↗

Bioenergy Feedstock Library Annual Summary Report 2024

The Bioenergy Feedstock Library (BFL), part of the Biomass Feedstock National User Facility (BFNUF) located at Idaho National Laboratory (INL), is a physical sample repository and a web-accessible electronic database. The BFL stores physical and chemical characteristics of biomass and waste carbon sources for energy use, as well as samples generated from U.S. Department of Energy (DOE) Bioenergy Technologies Office (BETO) and U.S. Department of Agriculture-funded projects. The objective of this Bioenergy Feedstock Library Annual Summary Report for 2024, similar to the 2023 Annual Summary Report , is to focus on the updates to: (1) publicly available analytical data and equipment tracked through the BFNUF, (2) significant increases in the physical samples available for request, (3) sample and data archival progress from recent BETO-funded projects, and (4) publicly available data sets created upon request from BETO, INL projects, or outside entities compared to the previous annual summary reports. This report highlights key statistics and available data and information important for INL, BFL users, academics, and industry.

09 BIOMASS FUELS↗

Technical Report Series on Global Modeling and Data Assimilation: Global Surface Ocean Carbon Estimates in a Model Forced by MERRA - Volume 31

MERRA products were used to force an established ocean biogeochemical model to estimate surface carbon inventories and fluxes in the global oceans. The results were compared to public archives of in situ carbon data and estimates. The model exhibited skill for ocean dissolved inorganic carbon (DIC), partial pressure of ocean CO2 (pCO2) and air-sea fluxes (FCO2). The MERRA-forced model produced global mean differences of 0.02% (approximately 0.3 microns) for DIC, -0.3% (about -1.2 (micro) atm; model lower) for pCO2, and -2.3% (-0.003 mol C/sq m/y) for FCO2 compared to in situ estimates. Basin-scale distributions were significantly correlated with observations for all three variables (r=0.97, 0.76, and 0.73, P<0.05, respectively for DIC, pCO2, and FCO2). All major oceanographic basins were represented as sources to the atmosphere or sinks in agreement with in situ estimates. However, there were substantial basin-scale and local departures.

Gregg, Watson W.↗

The Telecommunications and Data Acquisition Report

This quarterly publication provides archival reports on developments in programs in space communications, radio navigation, radio science, and ground-based radio and radar astronomy. It reports on activities of the Deep Space Network (DSN) in planning, supporting research and technology, implementation, and operations. Also included are standardization activities at the Jet Propulsion Laboratory for space data and information systems.

Yuen, Joseph H.↗

Life Sciences Data Archive Scientific Development

The Life Sciences Data Archive will provide scientists, managers and the general public with access to biomedical data collected before, during and after spaceflight. These data are often irreplaceable and represent a major resource from the space program. For these data to be useful, however, they must be presented with enough supporting information, description and detail so that an interested scientist can understand how, when and why the data were collected. The goal of this contract was to provide a scientific consultant to the archival effort at the NASA-Johnson Space Center. This consultant (Jay C. Buckey, Jr., M.D.) is a scientist, who was a co-investigator on both the Spacelab Life Sciences-1 and Spacelab Life Sciences-2 flights. In addition he was an alternate payload specialist for the Spacelab Life Sciences-2 flight. In this role he trained on all the experiments on the flight and so was familiar with the protocols, hardware and goals of all the experiments on the flight. Many of these experiments were flown on both SLS-1 and SLS-2. This background was useful for the archive, since the first mission to be archived was Spacelab Life Sciences-1. Dr. Buckey worked directly with the archive effort to ensure that the parameters, scientific descriptions, protocols and data sets were accurate and useful.

Buckey, Jay C., Jr.↗

A-Train Data Depot: Integrating and Exploring Data Along the A-Train Tracks

The immense potential for new science findings as a result of inter-instrument data analysis has led to the development of a new data portal at GSFC: the A-train Data Depot. The power and utility of this new service to the general public is amplified immensely when the archived data are used in conjunction with online data analysis services like Giovanni. This presentation details some of the challenges of data usage from multiple distinct missions and how the tool sets we have developed can help to overcome these challenges, considerably cut down on analysis overhead and promote science exploration in an otherwise very challenging arena.

Leptoukh, G.↗

Municipality of Anchorage Household Travel Survey

The 2002 Household Travel Survey for the municipality of Anchorage, Alaska, collected demographic, socioeconomic, and travel information about households and persons (age five and older) on an assigned, 24-hour travel period. The main objective of the study, conducted by NuStats, was to improve the transportation system. 2,035 households were recruited to participate in the study. Of these, 1,293 completed travel logs depicting detailed data on driving habits, such as purpose of the trips, time of the data, and mode of transportation—spanning from April 1, 2002, to May 17, 2002. This dataset is part of the Metropolitan Travel Survey Archive, which includes travel surveys from numerous public agencies across the United States and is archived by the Transportation Secure Data Center to ensure their continued public availability.

1Hz data↗

SHADOZ (Southern Hemisphere Additional Ozonesondes): A Tropical Ozonesonde-Radiosonde Network for the Atmospheric Community

A lack of sounding data has limited the accuracy of ozone satellite retrievals in the tropics and our understanding of chemical-dynamical interactions in a region strongly influenced by natural variability and anthropogenic activity. In 1998, NASA s Goddard Space Flight Center, NOAA's Climate Monitoring and Diagnostics Laboratory (CMDL) and a team of international sponsors established the SHADOZ (Southern Hemisphere ADditional OZonesondes) project to address the gap in tropical ozone soundings. SHADOZ augments launches at selected sites and provides a public archive of ozonesonde and radiosonde data from twelve tropical and subtropical stations at http://croc.gsfc.nasa.gov/shadoz. Instrumentation, data and a summary of the first scientific findings from SHADOZ are presented.

Thompson, Anne M.↗