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At least 73 records · Page 4

Arbuscular mycorrhizal fungal community responses to drought and nitrogen fertilization in switchgrass stands

Anthropogenic global change is increasing the severity and frequency of abiotic stresses such as drought that are likely to affect soil communities. Arbuscular mycorrhizal fungi (AMF) play important roles in many soil processes, so it is important to understand how drought affects AMF biodiversity. This is especially relevant in agricultural systems where crops rely on AMF associations for water and nutrient uptake, and where management decisions such as crop selection and fertilizer application may influence how the AMF community responds to drought. In this study, we examined the effects of reduced precipitation and nitrogen fertilization on AMF richness, community composition, and root and soil colonization in monocultures of two cultivars of switchgrass (Panicum virgatum) grown for bioenergy feedstock. We conducted a two-year field experiment using rain-out shelters to manipulate precipitation in mature stands of switchgrass growing in a long-term nitrogen fertilization (0 or 56 kg N ha –1 ) experiment at the W.K. Kellogg Biological Station Long-Term Ecological Research site in Michigan, USA. We expected that AMF richness and colonization would decrease due to drought, as predicted by the stress exclusion hypothesis. Contrary to our expectations, we found that drought stress increased AMF species richness in fertilized plots by 15%; there was no effect of drought on AMF richness in unfertilized plots. Drought also significantly altered AMF community composition, primarily due to increases in Rhizophagus taxa abundance, and reduced AMF root colonization in switchgrass by 6%. We also found variation in AMF richness and colonization across switchgrass cultivars as well as sampling dates. Furthermore, the changes in AMF richness and composition that we observed in this study may have implications for perennial bioenergy feedstock selection and management as changes in AMF communities may feedback to affect host plants.

54 ENVIRONMENTAL SCIENCES↗

Unveiling the mechanism of surface corrugation formation on a quasi free-standing bi-layer graphene via experimental and modeling investigations

Extensive studies have been conducted to accomplish the controls over the formation of surface corrugations on chemical vapor deposited (CVD) graphene. However, the underlying mechanism of the surface corrugation formation on graphene remains elusive, due to the difficulty delineating the growth-induced surface corrugations from wrinkles formed during graphene transfer with the use of a supporting layer casted on top of the sample. Here, in this study, “quasi free-standing” graphene was realized using the weakly-interacting h-BN layer during a wet transfer process to study the surface corrugation formation on graphene. The weak interaction of graphene with the supporting h-BN layer allows for the growth-induced surface corrugations to be released, enabling the investigation of the primary mechanism for the graphene corrugation behavior during the transfer process. The experimental measurements of surface corrugations were conducted using atomic force microscopy (AFM) to reveal that surface corrugations are formed following the graphene’s crystallographic directions. Density functional theory calculations using the projector augmented-wave (PAW) method and Perdew-Burke-Ernzerhof (PBE) exchange correlation functional were also conducted for two models of “atomic-scale ripples” and “nanoscale wrinkles” and showed that all of them are formed along the crystallographic axis of graphene, which is in good agreement with the experimental observations.

36 MATERIALS SCIENCE↗

Reducing Long‐Standing Surface Ozone Overestimation in Earth System Modeling by High‐Resolution Simulation and Dry Deposition Improvement

The overestimation of surface ozone concentration in low‐resolution global atmospheric chemistry and climate models has been a long‐standing issue. We first update the ozone dry deposition scheme in both high‐ (0.25°) and low‐resolution (1°) Community Earth System Model (CESM) version 1.3 runs, by adding the effects of leaf area index and correcting the sunlit and shaded fractions of stomatal resistances. With this update, 5‐year‐long summer simulations (2015–2019) using the low‐resolution CESM still exhibit substantial ozone overestimation (by 6.0–16.2 ppbv) over the U.S., Europe, eastern China, and ozone pollution hotspots. The ozone dry deposition scheme is further improved by adjusting the leaf cuticle conductance, reducing the mean ozone bias by 19%, and increasing the model resolution further reduces the ozone overestimation by 43%. We elucidate the mechanism by which model grid spacing influences simulated ozone, revealing distinctive pathways in urban versus rural areas. In rural areas, grid spacing mainly affects daytime ozone levels, where additional NO x emissions from nearby urban areas result in an ozone boost and overestimation in low‐resolution simulations. In contrast, over urban areas, daytime ozone overestimation follows a similar mechanism due to the influence of volatile organic compounds from surrounding rural areas. However, nighttime ozone overestimation is closely linked to weakened NO titration owing to the redistribution of urban NO x to rural areas. Additionally, stratosphere‐troposphere exchange may also contribute to reducing ozone bias in high‐resolution simulations, warranting further investigation. This optimized high‐resolution CESM may enhance understanding of ozone formation mechanisms, sources, and changes in a warming climate.

54 ENVIRONMENTAL SCIENCES↗

The Nature of Standing Plasma Waves Following Fast Breakdown in Thunderstorms

Narrow bipolar events (NBEs) are produced by large-scale systems of streamer corona discharges that occur inside thunderstorms and radiate intensely at very-high frequencies. Here we examine 14 high-power NBEs recorded in Florida which are followed by a lasting oscillatory signal. We isolate the dominant frequencies of the oscillations using empirical mode decomposition methods, and cross-reference this information with the radiator size inferred from broadband interferometry. We conclude that the oscillations are evidence of a standing electromagnetic wave in the plasma cavity created by the preceding streamer system, that is, the cavity's fundamental mode with wavelength equal to twice the radiator length. Contrasting the dispersive and attenuation properties of these waves across several different events allows us to conclude that the cavity is filled with ions resulting in an average conductivity of 0.01–0.1 mS/m, which are left behind after the streamers decay via electron attachment to oxygen molecules.

58 GEOSCIENCES↗

X-ray fluorescence standing wave study of the interaction of the antimicrobial peptide indolicidin with a supported model membrane

Indolicidin, a cationic antimicrobial peptide, interacts with lipid bilayers through electrostatic and hydrophobic interactions, disrupting microbial membranes. We investigated the depth-dependent localization of gold-nanoparticle-labeled indolicidin in a supported model membrane using X-ray fluorescence standing wave (SWXF) analysis. Liposomes composed of DMPC and DMPG were incubated with indolicidin labeled at its C-terminus with a 1.8 nm gold nanoparticle, then deposited onto a Si/Mo multilayer substrate via vesicle bursting. SWXF measurements revealed that at low peptide incubation concentrations (2–5 μM), gold-nanoparticle-labeled indolicidin remains primarily associated with the bilayer's outer leaflet. At higher concentrations (10 μM), the peptide penetrates deeper into the bilayer, with the labeled C-terminal region either localizing near the membrane's hydrophobic core or inducing membrane breakup. In conclusion, these findings suggest a concentration-dependent insertion mechanism.

Basnet, Gobind [Northern Illinois Univ., DeKalb, I↗

Improved optical standing-wave beam splitters for dilute Bose–Einstein condensates

Bose–Einstein condensate (BEC)-based atom interferometry exploits low temperatures and long coherence lengths to facilitate high-precision measurements. Progress in atom interferometry promises improvements in navigational devices like gyroscopes and accelerometers, as well as applications in fundamental physics such as accurate determination of physical constants. Previous work demonstrates that beam splitters and mirrors for coherent manipulation of dilute BEC momentum in atom interferometers can be implemented with sequences of non-resonant standing-wave light pulses. While previous work focuses on the optimization of the optical pulses’ amplitude and duration to produce high-order momentum states with high fidelity, we explore how varying the shape of the optical pulses affects optimal beam-splitter performance, as well as the effect of pulse shape on the sensitivity of optimized parameters in achieving high fidelity in high-momentum states. Here, in simulations of two-pulse beam splitters utilizing optimized square, triangle, and sinc-squared pulse shapes applied to dilute BECs, we, in some cases, reduce parameter sensitivity by an order of magnitude while maintaining fidelity.

74 ATOMIC AND MOLECULAR PHYSICS↗

Magnetoelastic standing waves induced in UO 2 by microsecond magnetic field pulses

Significance UO 2 is of particular interest due to its use as a nuclear fuel, its unusually poor thermal conductivity, and its uniquely strong magnetoelastic properties characterized by a linear coupling between strain and magnetization. We clearly observe mechanical standing waves and elastic resonances in a single crystal, induced by microsecond magnetic field pulses. The phase and amplitude of the mechanical resonances are modulated by the piezomagnetic switching behavior, offering a sensitive probe for otherwise hard to detect magnetic dynamics in UO 2 . We highlight the importance of magnetic domains in the attenuation of the mechanical resonances and the robustness of the antiferromagnetic state in UO 2 against externally applied magnetic fields to 150 T.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Saccharomycotina yeasts defy long-standing macroecological patterns

The Saccharomycotina yeasts (“yeasts” hereafter) are a fungal clade of scientific, economic, and medical significance. Yeasts are highly ecologically diverse, found across a broad range of environments in every biome and continent on earth; however, little is known about what rules govern the macroecology of yeast species and their range limits in the wild. Here, we trained machine learning models on 12,816 terrestrial occurrence records and 96 environmental variables to infer global distribution maps at ~1 km2 resolution for 186 yeast species (~15% of described species from 75% of orders) and to test environmental drivers of yeast biogeography and macroecology. We found that predicted yeast diversity hotspots occur in mixed montane forests in temperate climates. Diversity in vegetation type and topography were some of the greatest predictors of yeast species richness, suggesting that microhabitats and environmental clines are key to yeast diversity. We further found that range limits in yeasts are significantly influenced by carbon niche breadth and range overlap with other yeast species, with carbon specialists and species in high-diversity environments exhibiting reduced geographic ranges. Finally, yeasts contravene many long-standing macroecological principles, including the latitudinal diversity gradient, temperature-dependent species richness, and a positive relationship between latitude and range size (Rapoport’s rule). These results unveil how the environment governs the global diversity and distribution of species in the yeast subphylum. These high-resolution models of yeast species distributions will facilitate the prediction of economically relevant and emerging pathogenic species under current and future climate scenarios.

59 BASIC BIOLOGICAL SCIENCES↗

Equidistant optimization of elliptical superconducting rf standing wave cavities

A record accelerating rate was achieved earlier in standing wave (SW) SRF cavities when their shape was optimized for a lower peak surface magnetic field sacrificing the peak surface electric field. In view of new materials with higher limiting magnetic fields, expected for SRF cavities, in the first line of the Nb 3 Sn, the approach to optimization of cavity shape should be revised. A method of equidistant optimization, offered earlier for traveling wave (TW) cavities is applied to SW cavities. It is shown here that without limitation by magnetic field, the maximal accelerating rate is defined not only by limitations of the electric field but to a significant degree by the cavity shape. For example, for a cavity with the aperture radius of $R$ $a$ = 35 mm, the minimal ratio of the peak surface electric field to the accelerating rate is about $E$ pk / $E$ acc = 1.54. So, with the maximal surface field experimentally achieved $E$ pk ~ 125 MV / m, the maximal achievable accelerating rate is about 80 MeV / m even if there are no restrictions by the magnetic field. Optimized cavity shapes with and without limitations by a magnetic field are presented. Another opportunity—optimization for a low magnetic field, is opening for the same material, Nb 3 Sn, with the purpose of having a high-quality factor and increased accelerating rate that can be used for industrial linacs with cryocooler-based cooling scheme.

43 PARTICLE ACCELERATORS↗

Characterizing a supernova’s standing accretion shock instability with neutrinos and gravitational waves

Here, we perform a novel multi-messenger analysis for the identification and parameter estimation of the Standing Accretion Shock Instability (SASI) in a core collapse supernova with neutrino and gravitational wave (GW) signals. In the neutrino channel, this method performs a likelihood ratio test for the presence of SASI in the frequency domain. For gravitational wave signals we process an event with a modified constrained likelihood method. Using simulated supernova signals, the properties of the Hyper-Kamiokande neutrino detector, and O3 LIGO Interferometric data, we produce the two-dimensional probability density distribution (PDF) of the SASI activity indicator and calculate the probability of detection P D as well as the false identification probability P FI . We discuss the probability to establish the presence of the SASI as a function of the source distance in each observational channel, as well as jointly. Compared to a single-messenger approach, the joint analysis results in P D (at P FI = 0.1) of SASI activities that is larger by up to ≈ 40% for a distance to the supernova of 5 kpc. We also discuss how accurately the frequency and duration of the SASI activity can be estimated in each channel separately. Our methodology is suitable for implementation in a realistic data analysis and a multi-messenger setting.

79 ASTRONOMY AND ASTROPHYSICS↗

Multilevel analysis between Physcomitrium patens and Mortierellaceae endophytes explores potential long‐standing interaction among land plants and fungi

SUMMARY The model moss species Physcomitrium patens has long been used for studying divergence of land plants spanning from bryophytes to angiosperms. In addition to its phylogenetic relationships, the limited number of differential tissues, and comparable morphology to the earliest embryophytes provide a system to represent basic plant architecture. Based on plant–fungal interactions today, it is hypothesized these kingdoms have a long‐standing relationship, predating plant terrestrialization. Mortierellaceae have origins diverging from other land fungi paralleling bryophyte divergence, are related to arbuscular mycorrhizal fungi but are free‐living, observed to interact with plants, and can be found in moss microbiomes globally. Due to their parallel origins, we assess here how two Mortierellaceae species, Linnemannia elongata and Benniella erionia , interact with P. patens in coculture. We also assess how Mollicute ‐related or Burkholderia ‐related endobacterial symbionts (MRE or BRE) of these fungi impact plant response. Coculture interactions are investigated through high‐throughput phenomics, microscopy, RNA‐sequencing, differential expression profiling, gene ontology enrichment, and comparisons among 99 other P. patens transcriptomic studies. Here we present new high‐throughput approaches for measuring P. patens growth, identify novel expression of over 800 genes that are not expressed on traditional agar media, identify subtle interactions between P. patens and Mortierellaceae, and observe changes to plant–fungal interactions dependent on whether MRE or BRE are present. Our study provides insights into how plants and fungal partners may have interacted based on their communications observed today as well as identifying L. elongata and B. erionia as modern fungal endophytes with P. patens .

59 BASIC BIOLOGICAL SCIENCES↗

Physics-informed neural networks for identification of material properties using standing waves

A metallic structure in its initial stage of failure involves plastic deformation or environmental degradation that changes the elastic modulus and density. This work presents the detection of change in wave velocity (a function of elastic modulus and density) as a system identification problem. A physics-informed neural network (PINN) is proposed to solve the system identification problem. The PINN takes the spatial coordinates of scanning locations and time as inputs and provides the displacement and wave velocity as outputs. The governing partial differential equation of standing waves in a rod is incorporated into the neural network as physics in the form of a loss function. The wave velocity vector is randomly initiated. During the training of the network, physics is used to determine and update the wave velocity target vector from the network’s displacement predictions. The measured data, comprising sparse displacement response on the rod structure, are used to train the PINN. The wave velocity at the sparse locations on the rod is learned from the predicted displacements during the training. Using the predictions of the trained network, the response of free vibration or material property variation can be reconstructed at unscanned locations on the structure to obtain high-resolution maps for full-field imaging to detect and localize the changes caused by plastic deformation. The PINN’s sparse scanning and simultaneous prediction capability during training can lead to high scanning and data-processing speeds. This capability yields a nondestructive evaluation system that can predict the presence of degraded material locations as the structural vibrations are scanned and processed in real time.

Rathod, Vivek↗

Enhanced biochemical sensing with high- Q transmission resonances in free-standing membrane metasurfaces

Optical metasurfaces provide solutions to label-free biochemical sensing by localizing light resonantly beyond the diffraction limit, thereby selectively enhancing light–matter interactions for improved analytical performance. However, high-Q resonances in metasurfaces are usually achieved in the reflection mode, which impedes metasurface integration into compact imaging systems. Here, we demonstrate a metasurface platform for advanced biochemical sensing based on the physics of the bound states in the continuum (BIC) and electromagnetically induced transparency (EIT) modes, which arise when two interfering resonances from a periodic pattern of tilted elliptic holes overlap both spectrally and spatially, creating a narrow transparency window in the mid-infrared spectrum. We experimentally measure these resonant peaks observed in transmission mode (Q ~ 734 at λ ~ 8.8 µm) in free-standing silicon membranes and confirm their tunability through geometric scaling. We also demonstrate the strong coupling of the BIC-EIT modes with a thinly coated PMMA film on the metasurface, characterized by a large Rabi splitting (32 cm -1 ) and biosensing of protein monolayers in transmission mode. Our new photonic platform can facilitate the integration of metasurface biochemical sensors into compact and monolithic optical systems while being compatible with scalable manufacturing, thereby clearing the way for on-site biochemical sensing in everyday applications.

Rosas, Samir [Univ. of Wisconsin, Madison, WI (Uni↗

Daily water stable isotopes, transpiration, and matrix potential data for an aspen and engelmann stand in the East River Watershed (version 2)

We provide daily stable isotope (2H & 18O) ratios in soil water and xylem (plant stem) water, as well as the sap flow (transpiration) and the soil's matric potential at a forested site near Gothic, Colorado, in the East River catchment. We measured the stable isotopic composition of the transpiration and the daily transpiration flux sum of three aspen and three engelmann spruce. In both forest stands, we installed a soil profile and measured the soil matric potential at 15, 30, and 60 cm depth as well as the stable isotopes of soil pore water at 5, 10, 30, 60, and 90 cm depths. All isotope measurements were done in situ via vapor probes connected to a cavity ring down spectrometer (Picarro L1240i).We further report the daily meteorological data observed at billy barr near our study site. We also provide for each tree the relative share of root water uptake derived from the isotope measurements via a Bayesian mixing model (MixSIAR).The daily data is provided as a time series in "Iso_MP_Sap_DataDaily_ESSDiveUpload.csv" and the units are provided in "dd.csv"; the location of the instrumented trees and soil profiles are given as latitude and longitude coordinates saved as CSV and KMZ files; and a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata.The data was gathered to investigate the short-term changes of the water sources (i.e., variation of root water uptake from different soil depths) of the studied subalpine trees.Update 07/16/2025: The relative and absolute plant water uptake depths were grouped to ensure that the MixSIAR model was applied with endmembers that differed in their d2H value by at least 3 permill and at least 1 permill for d18O. Whenever the difference between observed d2H values for two or more probes at neighboring depths was less than 3 permill, we used the average value for the source water endmember. For days at which probes that were not next to each other measurements did not differ at least 3 permill, the average of all probes between these two depths was used as the water source endmember representing the depth range between these two probes.

54 ENVIRONMENTAL SCIENCES↗

Window Test Stand Status and Future Work [Slides]

Challenges to getting the window test stand running that were addressed. Initial design of half-anchor connector was insufficient. Accommodation of non-Rexolite windows required engineering.

42 ENGINEERING↗

Commissioning of Phase 1 of the LANL RFQ Test Stand - Beam Characterization

A series of numerical start-to-end simulations of Phase 1 of the 750 keV RFQ Test Stand which includes the ion source (H+ duoplasmatron), a 1-Solenoid LEBT, an emittance scan station and a Faraday cup were performed to find a mechanism for the characterization of the proton beam extracted from the duoplasmatron.

43 PARTICLE ACCELERATORS↗

Testing of PIP-II pre-production 650 MHz couplers in warm test stand and in cryomodule

650 MHz fundamental power couplers were developed for PIP-II project to deliver RF power for low-beta and high-beta elliptical cavities. Few prototypes were built and tested and after some modification we built 8 pre-production couplers (with few spares for vacuum side) for HB650 cryomodule. All couplers were successfully tested in pulse mode (up to 100kW) and in CW mode (up to 50kW) in test stand at full reflection at 8 phases. In baseline configuration with DC bias we do not see any multipactoring activity after short processing. We also tested power processing without bias for uncoated and TiN coated ceramic window. Results of these studies presented in this paper. One of the coupler was assembled on LB650 cavity and tested at cryogenic environment in STC cryostat at ~20kW power with full reflection at different reflection phase. We also demonstrated good result from power processing without bias for warm and cold cavity. Six couplers were assembled on HB650 cavities in pre-production cryomodule. Test results from cryomodule qualification is discussing in this paper.

43 PARTICLE ACCELERATORS↗

TPC Test-stands: An Overview & Future Prospects

The DUNE near detector design in Phase II will include a magnetized high-pressure gaseous-argon TPC (HPgTPC) surrounded by a calorimeter, called ND-GAr. ND-GAr is designed to provide fine-grained tracking, and due to its low detection threshold, it will be able to constrain one of the least-understood uncertainties for oscillation analysis: nuclear effects in argon at the neutrino interaction vertex. This talk will provide an overview of and an outlook about the ongoing R&D efforts in the context of the existing test stands that are evaluating the various readout system options for DUNE ND-GAr.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗