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At least 73 records · Page 4

Application of AVHRR vegetation index to study atmosphere-biosphere exchange of CO2: Results from a 3-D tracer transport model

The 'normalized difference vegetation indices' (NVI) derived from AVHRR radiances are combined with field data of soil respiration and a global map of net primary productivity to prescribe, for the globe, the seasonal exchange of CO2 between the atmosphere and the terrestrial biosphere. The monthly fluxes of CO2 thus obtained are used as inputs to a 3-D tracer transport model which uses winds generated by a 3-D atmospheric general circulation model to advect CO2 as an inert constituent. Analysis of the 3-D model results shows reasonable agreement between the simulated and observed annual cycles of atmospheric CO2 at the locations of the remote monitoring stations. The application is shown of atmospheric CO2 distributions to calibrate the NVI in terms of carbon fluxes. The approach suggests that the NVI may be used to provide quantitative information about long term and global scale variations of photosynthetic activity and of atmospheric CO2 concentrations provided that variations in the atmospheric circulation and in atmospheric composition are known.

Fung, Inez Y.

Atmosphere-biosphere exchange flux of carbon dioxide in a tallgrass prairie modeled with satellite spectral data

The estimation of the rate of net CO2 uptake of vegetated land surfaces is essential for studies of global carbon cycle. The present paper demonstrates the use of spectral reflectance data from satellite remote sensing to model net CO2 flux (NCF) of a tallgrass canopy at the Konza prairie, Kansas. A bidirectional reflectance canopy model was used to estimate seasonal changes in canopy leaf area index (LAI) from surface reflectances remotely sensed by SPOT 1 and Landsat 5 satellites. The radiation model was also coupled with leaf conductance-photosynthesis models to scale up stomatal conductance and NCF from individual leaves to canopy level according to radiation distribution inside the canopy. The satellite-data-driven model was able to closely simulate the seasonal change in LAI as well as the short-term variation of canopy LAI caused by the dry period during late July and early August in the area. Modeled canopy stomatal conductance (g(sub c)) and NCF agree with measurements within 0.16 cm/s and 0.28 mg m(exp -2)/s, respectively, during the growth season from late May to late August. In October both measured and modeled NCF turned to small negative values as canopy photosynthesis diminished and predicted LAI approached zero. In addition to data scatter, some of the differences between modeled and measured g(sub c) and NCF may be attributed to uncertainties in seasonal changes of plant physiological status that were not detected by satellite data; some of the differences were caused by inadequate description of the dependence of nighttime CO2 flux of soil respiration on near-surface turbulent mixing.

Gao, W.

BOREAS TF-1 SSA-OA Soil Characteristics Data

The BOREAS TF-1 team collected several data sets in support of its efforts to characterize and interpret soil information at the SSA-OA tower site in 1994 as part of BOREAS. Data sets collected include soil respiration, temperature, moisture, and gravimetric data. The data are stored in tabular ASCII format.

Black, T. Andrew

Carbon availability, soil pH, and microbial allocation to nitrogen acquisition shape grassland heterotrophic respiration in response to a decade of nitrogen addition

Previous work has found that anthropogenic inputs of nitrogen (N) and phosphorus (P) impact heterotrophic respiration during soil organic matter decomposition in grasslands, a critical pathway through which carbon (C) is lost from soil to the atmosphere. While N addition typically reduces heterotrophic respiration, why the strength and direction of this N effect varies among sites is unclear. Here, to address this, we conducted a 339-day laboratory incubation to measure heterotrophic respiration from nine grasslands across North America that have received 10 years of factorial N and P fertilization. N addition reduced cumulative respiration most at sites with low pH, low microbial allocation towards N acquisition, and high soil C concentration and availability. However, N addition had neutral rather than positive effects on heterotrophic respiration in sites with high pH and decomposer allocation towards N acquisition. Across sites, a decade of N addition reduced heterotrophic respiration by ∼24 %, driven by reductions in microbial biomass. Heterotrophic respiration was less sensitive to P addition, despite its increasing microbial biomass. However, simultaneous N and P addition did ameliorate negative N effects. These results show that previously observed variation in the response of heterotrophic respiration to N addition can be explained by soil C availability and pH status, widely measured factors which can be used to predict how grassland C fluxes may change under continuing nutrient deposition.

N addition

Patch-level CO2 and CH4 fluxes and porewater concentrations in experimental wetlands, 2 PPT saltwater intrusion simulations, Aug-Oct 2022: Louisiana

This dataset contains carbon dioxide (CO2) and methane (CH4) flux measurements from patches of wetland vegetation dominated by Typha domingensis and Panicum hemitomon, which were conducted to assess flux responses to acute saltwater intrusion. The measurements occurred before, during, and after simulated acute saltwater intrusion events of low concentrations of ~ 2 PPT. The measurements comprise gas fluxes from the wetland surface (i.e., soil-water column and vegetation) and fluxes from the soil-water column exclusively. These two sets of fluxes are separated into two files and are complemented with four more files containing porewater concentrations of CO2 and CH4 collected at 0-5 cm, 10-15 cm, and 20-25 cm depth increments, spectral indices measurements, biomass, and sediment elevation table measurements. The files can be opened with regular text editors or spreadsheet programs.

54 ENVIRONMENTAL SCIENCES

Patch-level CO2 and CH4 fluxes and porewater concentrations in experimental wetlands, 5 and 10 PPT saltwater intrusion simulations, Louisiana 2023-2024

This dataset containes carbon dioxide (CO2) and methane (CH4) flux measurements collected from wetland vegetation patches dominated by Typha domingensis and Panicum hemitomon to assess greenhouse gas flux responses to experimental saltwater intrusion (SWI) pulses. Measurements were conducted before, during, and after simulated SWI events at target salinities of approximately 5 parts per thousand (ppt) with durations of 6, 10, and 17 days and 10 ppt with a duration of 48 days, alongside a control wetland (with no salinity added, flood manipulation only). These data were generated to evaluate how the magnitude and duration of SWI alter wetland carbon exchange and related biogeochemical and plant responses. This data package includes flux measurements from the wetland surface (i.e, soil/water surface and enclosed vegetation) and from the soil/water surface only; porewater and surface water concentrations of CO2 and CH4; salinity, pH, electrical conductivity collected in porewater (at 5, 10, and 20 cm soil depths) and in surface water; soil redox potential; leaf spectral indices, leaf vapor pressure deficit, stomatal conductance; water level, salinity, and photosynthetically active radiation; and aboveground biomass.

EARTH SCIENCE > AGRICULTURE > SOILS > SOIL RESPIRA

Sub-10-Micron and Respirable Particles in Lunar Soils

Based on published lunar soil grain size distribution data, we estimate that 1-3% of the mass of typical mature lunar soils is comprised of grains less than 2.5 micrometers in diameter. These particles are in the respirable range (small enough to be inhaled). Estimates are used because the early methods of obtaining grain size distributions did not give reliable results below about 10 micrometers. Grain size analyses of Apollo 11 soil 10084 by a laser diffraction technique shows that this soil contains roughly 2% by volume in the respirable grain size, in agreement with our prior estimate.

Cooper, Bonnie L.

Soil viral production count, respiration, and amplicon data

This study aimed to quantify rates of viral production in aridisol soil under conditions as close to natural field soil as possible given the perturbations necessary to manipulate viral abundances. Viruses were removed from soil, then added back to virus-depleted soil to control the initial viral abundances at either 100% (field_abund) or 10% (reduced_abund) of measured field viral abundance to generate treatments with field-relevant and reduced viral infection pressure, respectively. Replicates of batch incubation jars were harvested every 8 hours for 48 hours to enumerate bacteria and viruses by microscopy (n=5) and profile bacterial community composition by 16S rRNA amplicon sequencing (n=3).

Zimmerman, Amy [Pacific Northwest National Laborat

Carbon Dynamics in Vegetation and Soils

The overall goals of CD-08 team in Phase I were to quantify the contributions of different components of the carbon cycle to overall ecosystem carbon balance in Amazonian tropical forests and to undertake process studies at a number of sites along the eastern LBA transect to understand how and why these fluxes vary with site, season, and year. We divided this work into a number of specific tasks: (1) determining the average rate (and variability) of tree growth over the past 3 decades; (2) determining age demographics of tree populations, using radiocarbon to determine tree age; (3) assessing the rate of production and decomposition of dead wood debris; (4) determining turnover rates for organic matter in soils and the mean age of C respired from soil using radiocarbon measurements; and (5) comparing our results with models and constructing models to predict the potential of tropical forests to function as sources or sinks of C. This report summarizes the considerable progress made towards our original goals, which have led to increased understanding of the potential for central Amazon forests to act as sources or sinks of carbon with altered productivity. The overall picture of tropical forest C dynamics emerging from our Phase I studies suggests that the fraction of gross primary production allocated to growth in these forests is only 25-30%, as opposed to the 50% assumed by many ecosystem models. Consequent slow tree growth rates mean greater mean tree age for a given diameter, as reflected in our measurements and models of tree age. Radiocarbon measurements in leaf and root litter suggest that carbon stays in living tree biomass for several years up to a decade before being added to soils, where decomposition is rapid. The time lags predicted from 14C, when coupled with climate variation on similar time scales, can lead to significant interannual variation in net ecosystem C exchange.

Trumbore, Susan

Validation Assessment for the Soil Moisture Active Passive (SMAP) Level 4 Carbon (L4_C) Data Product Version 5

The post-launch Cal/Val phase of the SMAP mission is guided by two primary objectives for each science product team: 1) to calibrate, verify, and improve the performance of the science algorithms, and 2) validate accuracies of the science data products as specified in the SMAP Level-1 mission science requirements. Algorithm science and product maintenance activities during the SMAP extended mission phase have also involved periodic algorithm calibration and product refinements to maintain or enhance product consistency and performance as well as science utility. This report provides an assessment of the latest (Version 5) SMAP Level 4 Carbon (L4_C) product. The L4_C Version 5 (v5) global record now spans more than six years (March 2015 – present) of SMAP operations and has benefited from five major reprocessing updates to the operational product. These reprocessing events and L4_C product release updates have incorporated various algorithm refinements and calibration adjustments to account for similar refinements to the upstream GEOS land model assimilation system, SMAP brightness temperatures, and MODIS vegetation inputs used for L4_C processing. The SMAP L4_C algorithms utilize a terrestrial carbon flux model informed by daily surface and root zone soil moisture information contributed from the SMAP Level 4 Soil Moisture (L4_SM) product along with optical remote sensing-based (e.g. MODIS-based) land cover and canopy fractional photosynthetic active radiation (fPAR), and other ancillary biophysical data. The carbon flux model estimates global daily net ecosystem CO2 exchange (NEE) and the component carbon fluxes, namely, vegetation gross primary production (GPP) and soil heterotrophic respiration (Rh). Other L4_C product elements include surface (~0-5 cm depth) soil organic carbon (SOC) stocks and associated environmental constraints to these processes, including soil moisture-related controls on GPP and ecosystem respiration (Kimball et al. 2014, Jones et al. 2017). The L4_C product addresses SMAP carbon cycle science objectives by: 1) providing a direct link between terrestrial carbon fluxes and underlying freeze/thaw and soil moisture-related constraints to these processes, 2) documenting primary connections between terrestrial water, energy and carbon cycles, and 3) improving understanding of terrestrial carbon sink activity. The SMAP L4_C algorithms and operational product are mature and at a CEOS Validation Stage 4 level (Jackson et al. 2012) based on extensive validation of the multi-year record against a diverse array of independent benchmarks, well characterized global performance, and systematic refinements gained from five major reprocessing events. There are no Level-1 mission science requirements for the L4_C product; however, self-imposed requirements have been established focusing on NEE as the primary product field for validation, and on demonstrating L4_C accuracy and success in meeting product science requirements (Jackson et al. 2012). The other L4_C product fields also have strong utility for carbon science applications (e.g., Liu et al. 2019, Endsley et al. 2020); however, analysis of these other fields is considered secondary relative to primary validation activities focusing on NEE. The L4_C targeted accuracy requirements are to meet or exceed a mean unbiased root-mean-square error (ubRMSE, or standard deviation of the error) for NEE of 1.6 g C m-2 d-1 and 30 g C m-2 yr-1, emphasizing northern (≥45°N) boreal and arctic ecosystems; this accuracy is similar to that of tower eddy covariance measurement-based observations (Baldocchi 2008). Methods used for the latest v5 L4_C product performance and validation assessment have been established from the SMAP Cal/Val plan and previous studies (Jackson et al. 2012, Jones et al. 2017) and include: 1) consistency evaluations of the product fields against earlier product releases (version 4 or earlier); 2) comparisons of daily carbon flux estimates with independent tower eddy 4covariance measurement-based daily carbon (CO2) flux observations from core tower validation sites (CVS); and 3) consistency checks against other global carbon products, including soil carbon inventory records, global GPP records derived from tower observation upscaling methods, and satellite-based observations of canopy solar induced chlorophyll fluorescence (SIF) as a surrogate for GPP. Metrics used to evaluate relative agreement between L4_C product fields and observational benchmarks include correlation (r-value), RMSE differences, bias and model sensitivity diagnostics. Following these validation criteria, the present report provides a validation assessment of the latest L4_C product release (v5). Detailed descriptions of the L4_C algorithm and additional global product accuracy and performance results are given elsewhere (Jones et al. 2017, Endsley et al. 2020). The v5 L4_C product replaces earlier product versions and continues to show: (i) accuracy and performance levels meeting or exceeding SMAP L4_C science requirements; (ii) improvement over the previous product version (version 4); and (iii) suitability for a diversity of science applications. Example L4_C applications from the recent literature include clarifying environmental trends and controls on the northern terrestrial carbon sink (Liu et al. 2019), diagnosing drought-related impacts on ecosystem productivity (Li et al. 2020), and regional monitoring of cropland conditions for projecting annual yields (Wurster et al. 2020). 2EXPECTED L4_C ALGORITHM AND PRODUCTPERFORMANCE The L4_C algorithm performance, including variance and uncertainty estimates of model outputs, was determined during the mission pre-launch phase through spatially explicit model sensitivity studies using available model inputs similar to those currently being used for operational production and evaluating the resulting model simulations over the observed range of northern (≥45 °N) and global conditions (Kimball et al. 2012, Entekhabi et al. 2014). The L4_C algorithm options were also evaluated during the mission prelaunch phase, including deriving canopy fPAR from lower order NDVI (Normalized Difference Vegetation Index) inputs in lieu of using MODIS (MOD15) fPAR; and including an explicit model representation of boreal fire disturbance recovery impacts. These results indicated that the L4_C accuracy requirements (i.e., NEE ubRMSE ≤ 30 g C m-2 yr-1or ≤ 1.6 g C m-2 d-1) could be met from the baseline algorithms over more than 82% and 89% of global and northern vegetated land areas, respectively (Yi et al. 2013, Kimball et al. 2014). The global L4_C algorithm error budget for NEE derived during the mission prelaunch phase indicated that the estimated NEE ubRMSE uncertainty is proportional to GPP and is therefore larger in higher biomass productivity areas, including forests and croplands (Kimball et al. 2014). Likewise, NEE ubRMSE uncertainty is expected to be lower in less-productive areas, including grasslands and shrublands. Expected model NEE ubRMSE levels were also generally within targeted accuracy levels for characteristically less-productive boreal and Arctic biomes, even though relative model error as a proportion of total productivity (NEE RMSE / GPP) may be large in these areas. The estimated NEE uncertainty was lower than expected in some warmer tropical high biomass productivity areas (e.g. Amazon rainforest) because of reduced low temperature and moisture constraints to the L4_C respiration calculations so that the bulk of model uncertainty is contributed by GPP in these areas. Model NEE uncertainty in the African Congo was estimated to

SMAP

Data for Regier et al. (2025), "Short-term experimental flooding impacts soil biogeochemistry but not aboveground vegetation in a coastal forest"

Rising sea levels and intensifying storms increase flooding pressure on coastal forests, but the mechanisms that drive coastal forest mortality remain unclear. This study used an ecosystem-scale manipulation (TEMPEST, Terrestrial Ecosystem Manipulation to Probe the Effects of Storm Treatments) to simulate hurricane-level flooding of a coastal forest and explore the individual and interactive impacts of inundation and salinity. This dataset comprises the results reported by Regier et al. (2025) in their paper "Short-term experimental flooding impacts soil biogeochemistry but not aboveground vegetation in a coastal forest." It consists of measurements of:- Belowground conductance- Soil dissolved oxygen and redox- Soil and tree greenhouse gas fluxes- Leaf photosynthesis, stomatal conductance, and intercellular carbon dioxide- Sap flux- Soil volumetric water content and electrical conductivityAll files are plain-text CSV (comma separated value) and no special software is required to read them.

54 ENVIRONMENTAL SCIENCES

Data from: Lowland Tropical Forests Remain a Methane Sink Under Warming and Long-Term Hurricane Disturbance Recovery

The repository folder contains spreadsheets and script for soil greenhouse gas (GHG) fluxes, soil moisture, soil temperature, air temperature, and precipitation measurements collected from the Tropical Responses to Altered Climate Experiment (TRACE) at the Sabana Research Field Station, El Yunque National Forest (USDA Forest Service; 18°19′28.74″ N, 65°43′50.09″ W) — an open-air field warming experiment located in a lowland tropical forest in Puerto Rico within the Luquillo Experimental Forest (LEF) — six to seven years after Hurricanes Irma and Maria (2017). All spreadsheets for soil and air microclimate data, as well as soil greenhouse gas data, are included as csv files. Air temperature data are also included as Excel spreadsheets (.xlsx). The script is built in R Studio, which is the only software required to run data analysis. This dataset is associated with the manuscript “Larocca Conte G ; Zuvela L ; Cruz-Pérez R ; Barreto-Vélez T ; Becerra-Santillan N ; Campbell S ; Chu H ; Dam T ; Grullón-Penkova I ; Kleit M ; Ortiz-Iglesias D ; Rubio-Lebrón L ; Cavaleri M ; Reed S ; Sihi D ; Wood T ; O'Connell C., 2026. Lowland Tropical Forests Remain a Methane Sink Under Warming and Long-Term Hurricane Disturbance Recovery. Agricultural and Forest Meteorology. In review". The dataset was used to test the effect of warming on soil CH4 dynamics following long-term legacy effects of hurricane disturbance. The dataset includes: - An overall README file in word and pdf format describing methodology and spreadsheets’ structure. - Continuous measurements of soil temperature and moisture from January 2023 to July 2024 measured with Campbell CS655 probes (“TRACE_soil_temperature_and_moisture_2023_cleaned(in).csv” and “TRACE_soil_temperature_and_moisture_2024_cleaned. csv”). - Air temperature data measured with a HOBO MX23O1A data logger (“Hobo air temperature 2023 Sep 2024” and “Hobo air temperature 2023 Sep 2024” – “CSV FILES folders”). - Precipitation data from a nearby weather tower downloaded from González et al. (2025; “sabana_2020-2025.csv”). - Soil CH4 and CO2 effluxes measured intermittently in two summer campaigns (June – August 2023 and June – July 2024) with a LI-COR 8200-01S Portable Smart Chamber coupled with a LI-COR LI-7810 CH4/ CO2/H2O Trace Gas Analyzer (“23_24COMBO2.0.csv”). - R markdown script for data analysis (“Trace new_PLOTS.Rmd”).

54 ENVIRONMENTAL SCIENCES

Wildfire‐Induced Losses of Soil Particulate and Mineral‐Associated Organic Carbon Persist for Over 4 Years in a Chaparral Ecosystem

ABSTRACT Wildfires can lower soil carbon (C) stocks directly through combustion, but also indirectly during post‐fire recovery if microbial C demands outpace photosynthetic C inputs. However, how much C is respired by soil microorganisms post‐fire may depend on wildfire effects on particulate organic carbon (POC; mostly plant material accessible to microbes) and/or mineral‐associated organic carbon (MAOC; considered C protected by minerals from decomposers), meaning assessment of wildfire impacts on these pools is necessary to predict microbial decomposition rates and, thus, the fate of soil C. Here, we measured POC, MAOC, pyrogenic organic matter C, plant cover, extracellular enzyme activity (EEA), and microbial community abundance and composition 17 days, and 1, 3, and 4 years after the Holy Fire burned 94 km 2 of fire‐adapted chaparral. The wildfire immediately decreased POC by 50% (from 51 ± 21 to 26 ± 6 g C kg −1 ) and MAOC by 33% (from 9.3 ± 0.9 to 6.3 ± 0.9 g C kg −1 ), consistent with MAOC being less vulnerable to loss than POC. POC decreased by another 38% 1 year post‐fire, consistent with increases in microbial abundance and EEA suggesting increased microbial decomposition. Between 1 and 4 years after the fire, cover of the dominant shrub (Arctostaphylos glandulosa) increased from 3.9% ± 1.6% to 16% ± 5.4% (compared to 58% ± 4.6% in unburned plots), marking the end of net soil C losses. Still, soil C did not increase between 1 and 4 years post‐fire, suggesting plant C inputs did not outpace microbial respiration, a finding consistent with isotopically heavier C from microorganisms raising bulk soil δ 13 C values. As global changes favor increases in wildfire frequency and severity, C losses via combustion and decomposition may outpace plant C inputs during the first 4 years post‐fire in chaparral, slowing the replenishment of soil C stocks.

Biodiversity & Conservation

A tale of two extremes: Temperature sensitivity of carbon loss from cool and hot soils

Soils represent the largest terrestrial carbon (C) pool, and the flux of carbon dioxide (CO 2 ) from soils to the atmosphere is ~ 6-10 times more than anthropogenic emissions. Understanding responses of soil CO 2 emissions to warming is crucial for evaluating feedback to ongoing environmental changes. The relationship between microbial respiration and temperature is typically modeled using a Q 10 function. Generally, observations of the apparent Q 10 of soil respiration are higher for cold vs. warm ecosystems, reflecting expected biophysical controls of Arrhenius kinetics. However, results from two field warming experiments in the tropics contradict this expectation, both observing extraordinarily high soil respiration responses to in situ warming. Our overall objective for the proposed work is to reduce uncertainty in temperature sensitivity of soil C loss by systematically synthesizing underlying mechanisms related to soil C turnover and stabilization. We are evaluating the temperature sensitivity of soil respiration in ecosystems across temperature extremes (e.g., arctic/boreal and tropical systems) by integrating data collected from field warming experiments with machine learning and biogeochemical models.

54 ENVIRONMENTAL SCIENCES

Zero Resistance Ammetry (ZRA) Measurements of Sediment Electrochemical Gradients, Old Woman Creek, Ohio, USA, June–November 2022

This dataset contains raw and processed zero resistance ammetry (ZRA) measurements collected from wetland sediments at Old Woman Creek, a freshwater estuary on Lake Erie, Ohio, USA, between June and November 2022. Measurements were obtained using a vertically deployed electrode array positioned at multiple depths within the sediment profile to capture electrochemical gradients associated with microbial activity and sediment geochemistry. The raw dataset consists of parsed instrument log files containing timestamps, electrode pair identifiers, and measured electrical potential (mV). The processed dataset includes standardized and quality-controlled values with instrument saturation limits removed and timestamps converted to ISO 8601 format. Electrode line identifiers were mapped to physical depths, enabling interpretation of depth-resolved electrochemical gradients. Instrument saturation values (−2048, −2047, 2047, and 2048 mV) were identified as measurement limits and excluded from quantitative analyses. All data parsing, processing, and quality control steps are documented in an accompanying R Markdown script, ensuring full reproducibility from raw instrument logs to final datasets.

EARTH SCIENCE > AGRICULTURE > SOILS > ELECTRICAL C

A Computational Workflow of Elucidating Viral Impact on Mediating Microbial Response to In-situ Experimental Warming: Bridging microbial modeling to carbon and mineral modeling

Viruses are abundant in soils and shape microbial communities in ways that can potentially influence ecosystem processes, yet their contributions to carbon cycling and mineral transformations remain poorly understood. Here we present a multi-phase framework that links virus-host interactions to soil biogeochemistry by combining ecological simulations, genome- and community-scale metabolic modeling, and statistical and machine-learning analyses. We first calibrated microbial abundance profiles under explicit infection scenarios to capture how viral pressure alters community structure, then explored alternative interaction strategies, including kill-the-winner, piggyback-the-winner, and mixed lytic-lysogenic modes, through forward simulations. These ecological shifts were translated into metabolic consequences using exchange fluxes summarized into biologically meaningful categories, while integrated statistical and machine-learning screens elevated subtle but consistent signals. Application of this framework revealed that viral infections shift the balance between organic and inorganic fluxes, redirecting metabolism from diffuse organic transformations toward inorganic pools such as protons and CO 2 , directly linking viral regulation to respiration and soil carbon balance. The roll-up analysis also isolated perturbations in critical mineral ions, including magnesium, manganese, zinc, and copper, which serve as essential enzymatic cofactors. In piggyback-the-winner scenarios, uptake of these ions was strongly suppressed. Contrasting viral strategies produced distinct community structures and metabolic outcomes, from broad suppression under kill-the-winner dynamics to dramatic redistributions under high-lytic and high-gain lysogenic regimes that collapsed vulnerable microbial populations while promoting opportunists. Together, these results provide a tractable path to trace viral perturbations from host abundance shifts to metabolic flux adjustments and ecosystem-scale processes, offering a practical way to include viruses in earth system models.

54 ENVIRONMENTAL SCIENCES

Response of soil nutrient pools and microbiomes to recurrent wildfire disturbance and varying burn severities in a mixed conifer forest

Wildfire is a pervasive disturbance in mixed-conifer forests, yet the relative influence of fire recurrence versus burn severity on soil biogeochemistry and microbial communities remains poorly quantified. We examined a natural gradient of fire history (0–3 prior fires) and burn severity (low–high) spanning 50 yr in a mixed-conifer ecosystem to assess how repeated fire shapes soil carbon (C) and nitrogen (N) pools, their isotopic signatures, mineral and particulate fractions, microbial community composition, carbon-use, CO₂ fluxes, and vegetation cover. Successive fires produced progressively higher bare-ground percentages and lower tree cover, which were tightly linked to declines in microbial diversity and reductions bulk %C, and %N. δ 13 C increased with fire frequency, indicating preferential loss of labile C through combustion or enhanced microbial oxidation, thereby explaining the observed net soil-C decline. Conversely, δ 15 N decreased and pH increased as tree density declined, reflecting altered N cycling and reduced acidification in post-fire soils. Fire recurrence, more than severity, corresponded with a marked shift in the bacterial community: for example, Xanthobacteraceae—key N-fixers and C-cyclers—diminished, while N-fixing Bacillaceae increased, underscoring the tightly coupled nature of soil nutrient dynamics and microbiome composition after repeated burns. Our results demonstrate that fire recurrence appears to be a stronger driver of post-fire soil ecosystem responses in this mixed-conifer forest, influencing both abiotic nutrient pools and the functional potential of the soil microbiome. These findings provide a more enhanced assessment and understanding to date of the biogeochemical consequences of repeated wildfire disturbance that can be used to inform management strategies aimed at preserving soil health in fire-prone landscapes.

54 ENVIRONMENTAL SCIENCES

Continental-scale integration of soil metagenomes and organic matter chemistry reveals ubiquitous microbial capacity for chemically-recalcitrant carbon decomposition

Soil organic matter (SOM) decomposition by microorganisms is a major uncertainty in predicting terrestrial carbon–atmosphere feedbacks, partly because we lack understanding of the microbial diversity involved in depolymerizing different carbon pools across environmental gradients. We address this gap using a continental-scale dataset pairing shotgun metagenomes with high-resolution SOM chemistry, assembling 0.76 Tbp of prokaryotic MAGs (828 genomes) and identifying 66,727 SOM molecules from 47 standardized U.S. soil cores selected using respiration rates from 106 soils. Integrating these datasets reveals widespread microbial potential for depolymerizing chemically-recalcitrant SOM previously considered stable. We uncover complementary metabolic specialization between genera affiliated with two abundant bacterial orders, Rhizobiales and Chthoniobacterales, and an archaeal order, Nitrososphaerales. This metabolic partitioning is consistent across soil depths and activity levels, suggesting coordinated decomposition of complex SOM through distinct but complementary biochemical strategies. The metabolic potential for depolymerization of chemically-recalcitrant compounds is supported by the abundance of these molecules across the soils, as indicated by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS), and by flux balance analysis of metabolic models. Our results show that a substantial portion of ostensibly stable SOM remains vulnerable to microbial decomposition, a mechanism not captured in current Earth System Models.

Song, Young C. [Pacific Northwest National Laborat